FvH4_3g25790

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014697
Physical Location & Seq
Forward (+)
18647433 .. 18651130
3698 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25790.t2

Sequence Viewer

Length: 1743 bp
ATGTCTTTGCAGACCCGCCAGGATTTGATCGAGAGGCAATGCAAGATCAGGAAGCGAGGCTGTTCGTCTTCGTCGTCTTCTTCTTTGGTGCGTAGGTACAGACTGAAGCGGGCCATTCTCGTCGGGAAGCGTGCCGGGTCGAGTACGCCGGTGCCTACTTGGAAGACTTCAACGAGCCCCAGATCGCCGGCCACCATGCCGAAGAAGCAAAAGGGTCTTCTTGTTCAGCAGCATTCCAAGGACGACGGGAAAGGGAAGGAAGTCATGTCAGTTTCAGCGAGGAAGCTCGCGGCTACGATGTGGGAGATCAATGAAGTTAATGATTTGAAAAGTAAAGAGTCCAAAATGAGAGAGGAGGAGGGTGTTCCTCAAATGCCGCCAAAATTGCCAGATCCATCATATTCTCCTATTTCAGAGAGGAAAACTGGATCTGGAGGTGGACACCAGAGAAGATTGTCGGCTGTTTCTCAGAGGATTCAACTGAGTGATCACTATTTGGGAGGATTTGAGACGCATAGCAGTCAAAGTATAATTGAGGTCAAAGATCATTCATATGGAAAAACTGATCGAAAATGCATCAAGACCTGTTTGAAGGAGGTCAGCAATGGCCTTTCTACATCCAAGGAGCTCGTAAAAGTTCTAACTCGTGTTTCTGGTCTTGAAGAACAGCAGCATTCTCTAAATATGCCCTTGCTCACAGCCTTACGAGCCGAACTTGATCGAGCGCGTATCCAGGTCCATCAATTGATCCAAGAACAGAGGTCAAACTGCAAAGAAATTGAGTACCTGATGAAGCATTTTGCAGAAGAAAAGGCAGCCTGGAAAAGCAAGGAGCGAGAGAAAATACGTGCTGCTGTAGCATGCATAGCAGAAGAACTTGAAGTAGAGAAGAAGCTGAGAAAACAAACAGAACGGTTGAATAAAAAGCTAGGCAAAGAACTGGAAGATAAAAATGTAGCTTTGTCAAAGGCAATAAAAGAACTTGAGAGGGAGAAGAGAGCAAAAGAGATTTTTGAGCAAGTTTGTGATGAGCTAGCTGTAGGTATGGGGGAAGACAGGGCCCAAGTTGAAGAACTAAAGAGAGAGTCGGAAAAAGTGCGTGAAGAGGTGGAGAAGGAGAGGGAAATGCTTCAGCTAGCAGATGTGTTGCGTGAGGAGAGAGTTCAGATGAAGCTCTCCGAAGCGAAGTACCATTTTGAAGAGAAAAATGCAGCTGTCGAGCAACTGAAGAATGAGCTAGAGGGCTACCTGAGATCCCATAAGGGTGAAGGCAGTGGTGACTCTCCAGACTTCAAAAGAATCAAGGAACTTGAGGCCTACTTAAAGAAAATAAATTTTGGGTCAATTCAGAGCATAAAGGAAGGCGGTGATGGAATGGAAGTTGCGTACAGAGAAGAGTGTGATGAAGAAGACTCAGGTGATGATCTTCATTCCATTGAACTAAACATGGACAATAGTAATAGGATGTACAAGTGGAGTTATGCTTGTGGAGACGATGCTGAAGATGATTCAAAAAGGAGTTCAGTTGATAAACAATTTAAAGGAAGAAGGTCTCTCTCTGAAAAGATACAATGGGAAAACATATGCTTAAACAAAAGTTCCACTGGCATTGATTGGGGATTTGGTTTGAAAACACAAGAACATGTAGACGAGCATCAAACACAAGAACGCGAAACTGAAATCAAGACTAACGGATCTGCTATATGTCTCATAGACCATATCTTATCTGATCCGAAAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

581

Amino Acids

66.21

Weight (kDa)

8.11

Isoelectric Point (pI)

56.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014697)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g25790 FvH4_3g25790
malus_domestica MD03G1150600.v1.1 MD11G1170000.v1.1
prunus_persica Prupe.6G137500_v2.0.a1
pyrus_communis pycom03g10470 pycom11g14240
rosa_chinensis RchiOBHm_Chr5g0047011
rosa_laevigata RLG00000034458
rosa_multiflora Rmu_sc0005544.1_g000006
rosa_roxburghii Rroxscaffold_1G00034420
rosa_rugosa Rorug05G0233200
rosa_samantha Rh5AG312400 Rh5BG321500 Rh5CG347700 Rh5DG332400
rosa_wichuraiana Rw5G029200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 151
AccI GTMKAC 1 cut(s) 1647
AccII CGCG 3 cut(s) 290, 727, 1671
AciI CCGC 5 cut(s) 16, 109, 290, 377, 1365
AclWI GGATC 6 cut(s) 386, 436, 742, 1248, 1702, 1724
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 1 cut(s) 1333
AcuI CTGAAG 4 cut(s) 125, 1115, 1247, 1521
AfaI GTAC 6 cut(s) 98, 145, 785, 1190, 1388, 1469
AflIII ACRYGT 1 cut(s) 1642
AjnI CCWGG 3 cut(s) 18, 732, 818
Alw21I GWGCWC 1 cut(s) 630
Alw26I GTCTC 4 cut(s) 503, 1485, 1557, 1712
AlwI GGATC 6 cut(s) 386, 436, 742, 1248, 1702, 1724
AoxI GGCC 5 cut(s) 111, 189, 607, 1059, 1314
ApaI GGGCCC 1 cut(s) 1063
ApeKI GCWGC 5 cut(s) 229, 670, 815, 851, 1211
ApoI RAATTY 1 cut(s) 1333
Asp700I GAANNNNTTC 1 cut(s) 1128
AspLEI GCGC 1 cut(s) 727
AspS9I GGNCC 4 cut(s) 111, 736, 1059, 1060
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 4 cut(s) 1277, 1289, 1379, 1430
AsuNHI GCTAGC 2 cut(s) 1033, 1135
AvaII GGWCC 1 cut(s) 736
BaeGI GKGCMC 1 cut(s) 1063
BanI GGYRCC 1 cut(s) 151
BanII GRGCYC 3 cut(s) 179, 630, 1063
BauI CACGAG 1 cut(s) 645
BbsI GAAGAC 6 cut(s) 60, 69, 170, 209, 1059, 1416
Bbv12I GWGCWC 1 cut(s) 630
BbvI GCAGC 5 cut(s) 241, 682, 827, 838, 1223
BccI CCATC 3 cut(s) 403, 747, 1364
BciT130I CCWGG 3 cut(s) 20, 734, 820
BciVI GTATCC 1 cut(s) 740
BclI TGATCA 1 cut(s) 487
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 4 cut(s) 503, 1485, 1557, 1712
BfaI CTAG 4 cut(s) 929, 1034, 1136, 1238
BfmI CTRYAG 2 cut(s) 855, 1038
BfuI GTATCC 1 cut(s) 740
BisI GCNGC 7 cut(s) 230, 291, 377, 671, 816, 852, 1212
BlsI GCNGC 7 cut(s) 231, 292, 378, 672, 817, 853, 1213
Bme1390I CCNGG 4 cut(s) 20, 136, 734, 820
Bme18I GGWCC 1 cut(s) 736
BmgT120I GGNCC 4 cut(s) 111, 736, 1059, 1060
BmiI GGNNCC 2 cut(s) 153, 1061
BmrFI CCNGG 4 cut(s) 20, 136, 734, 820
BmsI GCATC 3 cut(s) 585, 1486, 1663
BmtI GCTAGC 2 cut(s) 1037, 1139
BpiI GAAGAC 6 cut(s) 60, 69, 170, 209, 1059, 1416
BpmI CTGGAG 2 cut(s) 453, 1269
BpuEI CTTGAG 2 cut(s) 1004, 1331
BpuMI CCSGG 1 cut(s) 136
BsaAI YACGTR 1 cut(s) 848
BsaI GGTCTC 1 cut(s) 1557
BsaJI CCNNGG 2 cut(s) 237, 621
Bse118I RCCGGY 2 cut(s) 148, 187
Bse1I ACTGG 3 cut(s) 430, 945, 1609
Bse3DI GCAATG 2 cut(s) 44, 610
BseBI CCWGG 3 cut(s) 20, 734, 820
BseDI CCNNGG 2 cut(s) 237, 621
BseGI GGATG 2 cut(s) 617, 1470
BseMI GCAATG 2 cut(s) 44, 610
BseMII CTCAG 5 cut(s) 473, 482, 887, 1241, 1428
BseNI ACTGG 3 cut(s) 430, 945, 1609
BseRI GAGGAG 3 cut(s) 368, 371, 1169
BseSI GKGCMC 1 cut(s) 1063
BseXI GCAGC 5 cut(s) 241, 682, 827, 838, 1223
Bsh1236I CGCG 3 cut(s) 290, 727, 1671
BshFI GGCC 5 cut(s) 113, 191, 609, 1061, 1316
BshNI GGYRCC 1 cut(s) 151
BsiHKAI GWGCWC 1 cut(s) 630
BsiSI CCGG 3 cut(s) 135, 149, 188
BsmAI GTCTC 4 cut(s) 503, 1485, 1557, 1712
BsmBI CGTCTC 2 cut(s) 503, 1485
BsmI GAATGC 2 cut(s) 232, 673
BsnI GGCC 5 cut(s) 113, 191, 609, 1061, 1316
Bso31I GGTCTC 1 cut(s) 1557
Bsp120I GGGCCC 1 cut(s) 1059
Bsp1286I GDGCHC 3 cut(s) 179, 630, 1063
Bsp1407I TGTACA 1 cut(s) 1467
BspACI CCGC 5 cut(s) 16, 109, 290, 377, 1365
BspANI GGCC 5 cut(s) 113, 191, 609, 1061, 1316
BspCNI CTCAG 5 cut(s) 474, 481, 888, 1242, 1427
BspFNI CGCG 3 cut(s) 290, 727, 1671
BspLI GGNNCC 2 cut(s) 153, 1061
BspOI GCTAGC 2 cut(s) 1037, 1139
BspPI GGATC 6 cut(s) 386, 436, 742, 1248, 1702, 1724
BspT107I GGYRCC 1 cut(s) 151
BspTNI GGTCTC 1 cut(s) 1557
BsrDI GCAATG 2 cut(s) 44, 610
BsrFI RCCGGY 2 cut(s) 148, 187
BsrGI TGTACA 1 cut(s) 1467
BsrI ACTGG 3 cut(s) 430, 945, 1609
BssAI RCCGGY 2 cut(s) 148, 187
BssECI CCNNGG 2 cut(s) 237, 621
BssSI CACGAG 1 cut(s) 645
BssT1I CCWWGG 2 cut(s) 237, 621
Bst2BI CACGAG 1 cut(s) 645
Bst2UI CCWGG 3 cut(s) 20, 734, 820
Bst4CI ACNGT 1 cut(s) 915
Bst6I CTCTTC 4 cut(s) 989, 1098, 1194, 1389
BstAUI TGTACA 1 cut(s) 1467
BstBAI YACGTR 1 cut(s) 848
BstC8I GCNNGC 7 cut(s) 111, 132, 189, 288, 862, 1035, 1137
BstDEI CTNAG 5 cut(s) 468, 482, 896, 1250, 1414
BstF5I GGATG 2 cut(s) 617, 1470
BstFNI CGCG 3 cut(s) 290, 727, 1671
BstHHI GCGC 1 cut(s) 727
BstMAI GTCTC 4 cut(s) 503, 1485, 1557, 1712
BstMWI GCNNNNNNNGC 5 cut(s) 205, 385, 707, 857, 866
BstNI CCWGG 3 cut(s) 20, 734, 820
BstNSI RCATGY 2 cut(s) 864, 1646
BstSCI CCNGG 4 cut(s) 18, 134, 732, 818
BstSFI CTRYAG 2 cut(s) 855, 1038
BstSLI GKGCMC 1 cut(s) 1063
BstUI CGCG 3 cut(s) 290, 727, 1671
BstV1I GCAGC 5 cut(s) 241, 682, 827, 838, 1223
BstV2I GAAGAC 6 cut(s) 60, 69, 170, 209, 1059, 1416
BstX2I RGATCY 4 cut(s) 391, 428, 1253, 1694
BstYI RGATCY 4 cut(s) 391, 428, 1253, 1694
BsuI GTATCC 1 cut(s) 740
BsuRI GGCC 5 cut(s) 113, 191, 609, 1061, 1316
BtsCI GGATG 2 cut(s) 617, 1470
BtsI GCAGTG 1 cut(s) 1279
BtsIMutI CAGTG 2 cut(s) 1279, 1602
Cac8I GCNNGC 7 cut(s) 111, 132, 189, 288, 862, 1035, 1137
CfoI GCGC 1 cut(s) 727
Cfr10I RCCGGY 2 cut(s) 148, 187
Cfr13I GGNCC 4 cut(s) 111, 736, 1059, 1060
CseI GACGC 1 cut(s) 520
Csp6I GTAC 6 cut(s) 97, 144, 784, 1189, 1387, 1468
CspCI CAANNNNNGTGG 2 cut(s) 1591, 1626
CviAII CATG 5 cut(s) 196, 265, 861, 1447, 1643
CviQI GTAC 6 cut(s) 97, 144, 784, 1189, 1387, 1468
DdeI CTNAG 5 cut(s) 468, 482, 896, 1250, 1414
DraI TTTAAA 1 cut(s) 1540
EaeI YGGCCR 1 cut(s) 189
Eam1104I CTCTTC 4 cut(s) 989, 1098, 1194, 1389
EarI CTCTTC 4 cut(s) 989, 1098, 1194, 1389
Ecl136II GAGCTC 1 cut(s) 628
Eco130I CCWWGG 2 cut(s) 237, 621
Eco147I AGGCCT 1 cut(s) 1316
Eco24I GRGCYC 3 cut(s) 179, 630, 1063
Eco31I GGTCTC 1 cut(s) 1557
Eco47I GGWCC 1 cut(s) 736
Eco53kI GAGCTC 1 cut(s) 628
Eco57I CTGAAG 4 cut(s) 125, 1115, 1247, 1521
EcoICRI GAGCTC 1 cut(s) 628
EcoO109I RGGNCCY 1 cut(s) 1059
EcoRII CCWGG 3 cut(s) 18, 732, 818
EcoT14I CCWWGG 2 cut(s) 237, 621
EcoT22I ATGCAT 2 cut(s) 578, 866
EcoT38I GRGCYC 3 cut(s) 179, 630, 1063
ErhI CCWWGG 2 cut(s) 237, 621
Esp3I CGTCTC 2 cut(s) 503, 1485
FaeI CATG 5 cut(s) 199, 268, 864, 1450, 1646
FatI CATG 5 cut(s) 195, 264, 860, 1446, 1642
FauI CCCGC 2 cut(s) 23, 102
FauNDI CATATG 2 cut(s) 553, 1583
FbaI TGATCA 1 cut(s) 487
FblI GTMKAC 1 cut(s) 1647
Fnu4HI GCNGC 7 cut(s) 230, 291, 377, 671, 816, 852, 1212
FokI GGATG 2 cut(s) 604, 1477
FriOI GRGCYC 3 cut(s) 179, 630, 1063
Fsp4HI GCNGC 7 cut(s) 230, 291, 377, 671, 816, 852, 1212
FspBI CTAG 4 cut(s) 929, 1034, 1136, 1238
GlaI GCGC 1 cut(s) 726
GluI GCNGC 7 cut(s) 230, 291, 377, 671, 816, 852, 1212
GsuI CTGGAG 2 cut(s) 453, 1269
HaeIII GGCC 5 cut(s) 113, 191, 609, 1061, 1316
HapII CCGG 3 cut(s) 135, 149, 188
HgaI GACGC 1 cut(s) 520
HhaI GCGC 1 cut(s) 727
Hin1II CATG 5 cut(s) 199, 268, 864, 1450, 1646
Hin6I GCGC 1 cut(s) 725
HinP1I GCGC 1 cut(s) 725
HinfI GANTC 7 cut(s) 338, 475, 1085, 1280, 1299, 1412, 1508
HpaII CCGG 3 cut(s) 135, 149, 188
HphI GGTGA 4 cut(s) 1277, 1289, 1379, 1430
Hpy166II GTNNAC 2 cut(s) 440, 1648
Hpy188I TCNGA 9 cut(s) 415, 471, 1090, 1167, 1180, 1350, 1561, 1729, 1734
Hpy188III TCNNGA 8 cut(s) 31, 49, 124, 432, 580, 659, 1286, 1684
Hpy8I GTNNAC 2 cut(s) 440, 1648
Hpy99I CGWCG 3 cut(s) 76, 125, 248
HpyAV CCTTC 6 cut(s) 250, 586, 1108, 1262, 1355, 1542
HpyCH4III ACNGT 1 cut(s) 915
HpyCH4IV ACGT 1 cut(s) 847
HpyCH4V TGCA 7 cut(s) 10, 42, 576, 771, 803, 864, 1211
HpyF10VI GCNNNNNNNGC 5 cut(s) 205, 385, 707, 857, 866
HpyF3I CTNAG 5 cut(s) 468, 482, 896, 1250, 1414
HpySE526I ACGT 1 cut(s) 847
Hsp92II CATG 5 cut(s) 199, 268, 864, 1450, 1646
HspAI GCGC 1 cut(s) 725
KroI GCCGGC 1 cut(s) 187
KroNI GCCGGC 1 cut(s) 189
Ksp22I TGATCA 1 cut(s) 487
LmnI GCTCC 2 cut(s) 625, 832
Lsp1109I GCAGC 5 cut(s) 241, 682, 827, 838, 1223
LweI GCATC 3 cut(s) 585, 1486, 1663
MaeI CTAG 4 cut(s) 929, 1034, 1136, 1238
MaeII ACGT 1 cut(s) 847
MaeIII GTNAC 1 cut(s) 1277
MfeI CAATTG 1 cut(s) 743
MflI RGATCY 4 cut(s) 391, 428, 1253, 1694
MhlI GDGCHC 3 cut(s) 179, 630, 1063
MluCI AATT 7 cut(s) 383, 531, 743, 777, 1333, 1344, 1535
MlyI GAGTC 4 cut(s) 347, 1094, 1274, 1406
MmeI TCCRAC 1 cut(s) 1068
Mph1103I ATGCAT 2 cut(s) 578, 866
MroNI GCCGGC 1 cut(s) 187
MroXI GAANNNNTTC 1 cut(s) 1128
MseI TTAA 4 cut(s) 318, 1322, 1539, 1589
MslI CAYNNNNRTG 2 cut(s) 552, 1263
MspA1I CMGCKG 1 cut(s) 1214
MspI CCGG 3 cut(s) 135, 149, 188
MspR9I CCNGG 4 cut(s) 20, 136, 734, 820
MunI CAATTG 1 cut(s) 743
Mva1269I GAATGC 2 cut(s) 232, 673
MvaI CCWGG 3 cut(s) 20, 734, 820
MvnI CGCG 3 cut(s) 290, 727, 1671
MwoI GCNNNNNNNGC 5 cut(s) 205, 385, 707, 857, 866
NaeI GCCGGC 1 cut(s) 189
NciI CCSGG 1 cut(s) 136
NdeI CATATG 2 cut(s) 553, 1583
NgoMIV GCCGGC 1 cut(s) 187
NheI GCTAGC 2 cut(s) 1033, 1135
NlaIII CATG 5 cut(s) 199, 268, 864, 1450, 1646
NlaIV GGNNCC 2 cut(s) 153, 1061
NmuCI GTSAC 1 cut(s) 1277
NsiI ATGCAT 2 cut(s) 578, 866
NspI RCATGY 2 cut(s) 864, 1646
PaeI GCATGC 1 cut(s) 864
PceI AGGCCT 1 cut(s) 1316
PciI ACATGT 1 cut(s) 1642
PcsI WCGNNNNNNNCGW 1 cut(s) 71
PctI GAATGC 2 cut(s) 232, 673
PdiI GCCGGC 1 cut(s) 189
PdmI GAANNNNTTC 1 cut(s) 1128
PfeI GAWTC 3 cut(s) 475, 1299, 1508
PkrI GCNGC 7 cut(s) 231, 292, 378, 672, 817, 853, 1213
PleI GAGTC 4 cut(s) 346, 1093, 1274, 1406
PpsI GAGTC 4 cut(s) 346, 1093, 1274, 1406
Ppu21I YACGTR 1 cut(s) 848
PscI ACATGT 1 cut(s) 1642
Psp124BI GAGCTC 1 cut(s) 630
Psp6I CCWGG 3 cut(s) 18, 732, 818
PspGI CCWGG 3 cut(s) 18, 732, 818
PspN4I GGNNCC 2 cut(s) 153, 1061
PspOMI GGGCCC 1 cut(s) 1059
PspPI GGNCC 4 cut(s) 111, 736, 1059, 1060
PsuI RGATCY 4 cut(s) 391, 428, 1253, 1694
PvuII CAGCTG 1 cut(s) 1214
RsaI GTAC 6 cut(s) 98, 145, 785, 1190, 1388, 1469
RsaNI GTAC 6 cut(s) 97, 144, 784, 1189, 1387, 1468
RseI CAYNNNNRTG 2 cut(s) 552, 1263
SacI GAGCTC 1 cut(s) 630
SaqAI TTAA 4 cut(s) 318, 1322, 1539, 1589
SatI GCNGC 7 cut(s) 230, 291, 377, 671, 816, 852, 1212
Sau96I GGNCC 4 cut(s) 111, 736, 1059, 1060
SchI GAGTC 4 cut(s) 347, 1094, 1274, 1406
ScrFI CCNGG 4 cut(s) 20, 136, 734, 820
SduI GDGCHC 3 cut(s) 179, 630, 1063
SfaNI GCATC 3 cut(s) 585, 1486, 1663
SfcI CTRYAG 2 cut(s) 855, 1038
SgrAI CRCCGGYG 1 cut(s) 148
SinI GGWCC 1 cut(s) 736
SmiMI CAYNNNNRTG 2 cut(s) 552, 1263
SmlI CTYRAG 2 cut(s) 983, 1310
SmoI CTYRAG 2 cut(s) 983, 1310
SphI GCATGC 1 cut(s) 864
Sse9I AATT 7 cut(s) 383, 531, 743, 777, 1333, 1344, 1535
SseBI AGGCCT 1 cut(s) 1316
SsiI CCGC 5 cut(s) 16, 109, 290, 377, 1365
SspMI CTAG 4 cut(s) 929, 1034, 1136, 1238
SstI GAGCTC 1 cut(s) 630
StuI AGGCCT 1 cut(s) 1316
StyD4I CCNGG 4 cut(s) 18, 134, 732, 818
StyI CCWWGG 2 cut(s) 237, 621
TaaI ACNGT 1 cut(s) 915
TaiI ACGT 1 cut(s) 850
TaqI TCGA 5 cut(s) 30, 140, 568, 721, 1218
TasI AATT 7 cut(s) 383, 531, 743, 777, 1333, 1344, 1535
TatI WGTACW 1 cut(s) 1467
TauI GCSGC 2 cut(s) 293, 379
TfiI GAWTC 3 cut(s) 475, 1299, 1508
Tru1I TTAA 4 cut(s) 318, 1322, 1539, 1589
Tru9I TTAA 4 cut(s) 318, 1322, 1539, 1589
TscAI CASTG 2 cut(s) 1279, 1609
TseFI GTSAC 1 cut(s) 1277
TseI GCWGC 5 cut(s) 229, 670, 815, 851, 1211
Tsp45I GTSAC 1 cut(s) 1277
TspDTI ATGAA 6 cut(s) 327, 540, 806, 1184, 1418, 1419
TspGWI ACGGA 1 cut(s) 1707
TspRI CASTG 2 cut(s) 1279, 1609
VpaK11BI GGWCC 1 cut(s) 736
XapI RAATTY 1 cut(s) 1333
XceI RCATGY 2 cut(s) 864, 1646
XmiI GTMKAC 1 cut(s) 1647
XmnI GAANNNNTTC 1 cut(s) 1128
XspI CTAG 4 cut(s) 929, 1034, 1136, 1238
Zsp2I ATGCAT 2 cut(s) 578, 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.