Rh5AG312400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0014697
Physical Location & Seq
Forward (+)
45159251 .. 45162832
3582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG312400.1

Sequence Viewer

Length: 1233 bp
ATGTTGATTTCATATCACAATATGCAGGTCAAAGATGAATCATATGGAAAAACTGATCGAAAATGCATTAAGACCTGTTTGAAGGAGGTCAGCAATGGCCTTTCTACATCCAAGGAGCTCGTAAAAGTTCTAACTCGTGTTTCTGGTCTTGAAGAGCAGCAGCATTCTCTAAGTATGCCCCTGCTCTCAGCCTTACGAGTTGAACTTGATCGAGCACGCATCCAGGTTCATCAATTGATCCGAGAACAGAGGTCAAACTGCAAAGAAATTGAGTACCTCATGAAGCATTTTGCAGAAGAAAAGGCAGCCTGGAAAAGAAAGGAGCGAGAGAAAATACGTGCTGCTGTAGCATGCATAGCAGAAGAACTTGAAGTAGAGAAGAAGCTGAGAAAACAAACAGAACGGTTGAACAAAAAGCTTGGCAAAGAACTGGAAGATAAAAATGTAGCTCTGTCAAAGGCAACAAAAGAACTTGAGAGGGAGAAGAGAGCAAAAGAGATTTTTGAGCAAGTTTGTGATGAGTTAGCCGTAGGTATGGGGGAAGACAGGGCCCAAGTTGAAGAACTAAAGAGAGAGTCAGAAAAAGTTCGTGAAGAGGTGGAAAAGGAGAGGGAAATGCTTCAGCTAGCAGATGTGTTGCGCGAGGAGAGAGTTCAGATGAAGCTCTCGGAAGCAAAGTACCATTTTGAAGAAAAAAATGCGGCTGTTGAGCAACTGAAGAATGAGCTAGAGGGCTACTTGAGATCCCATAAGGGTGAAGGCAGTGGTGAGTCTCCAGACTTCAAAAGAATCAAGGAACTTGAGGCCTACTTAAAGAAAATAAATTTTGGGTCATTTCAGAGCATAAAGGAAGGAGACGATGGAATGGAAGTTGCATACAGAGAAGAGTGTGATGAAGAAGATTCAGGTGATGATCTTCATTCCATTGAACTAAACATGGACAATGGTAATAGAAGCTACAAGTGGAGTTATGCTTGTGGAGATGATGCTGAAGATGATTCAAAAAGGAGTTCAGTTGATAAACAATTTAAAGGAAGAAGGTCTCTCTCTGAAAAGATACAATGGGAAAACATATGCTTAAACAAAAGTTCCACCGGCATTGATTGGGGATTTGGCTCAAAAACTCAAGAACATGCAGATGGGCATCAAACACAAGAACACGAAACTGAAATCAAGAAAAATGGATCTGCTGTATGTCTCTTAGACCATATCTTATATGATTCAAAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

410

Amino Acids

47.46

Weight (kDa)

5.63

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014697)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g25790 FvH4_3g25790
malus_domestica MD03G1150600.v1.1 MD11G1170000.v1.1
prunus_persica Prupe.6G137500_v2.0.a1
pyrus_communis pycom03g10470 pycom11g14240
rosa_chinensis RchiOBHm_Chr5g0047011
rosa_laevigata RLG00000034458
rosa_multiflora Rmu_sc0005544.1_g000006
rosa_roxburghii Rroxscaffold_1G00034420
rosa_rugosa Rorug05G0233200
rosa_samantha Rh5AG312400 Rh5BG321500 Rh5CG347700 Rh5DG332400
rosa_wichuraiana Rw5G029200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 16
AccII CGCG 1 cut(s) 642
AciI CCGC 1 cut(s) 701
AclWI GGATC 3 cut(s) 232, 738, 1192
AcsI RAATTY 1 cut(s) 823
AcuI CTGAAG 3 cut(s) 605, 737, 1011
AfaI GTAC 2 cut(s) 275, 680
AjnI CCWGG 2 cut(s) 222, 308
AluBI AGCT 8 cut(s) 118, 385, 418, 449, 625, 664, 727, 957
AluI AGCT 8 cut(s) 118, 385, 418, 449, 625, 664, 727, 957
Alw21I GWGCWC 2 cut(s) 120, 217
Alw26I GTCTC 4 cut(s) 777, 849, 1047, 1202
AlwI GGATC 3 cut(s) 232, 738, 1192
AoxI GGCC 3 cut(s) 97, 549, 804
ApaI GGGCCC 1 cut(s) 553
ApeKI GCWGC 4 cut(s) 157, 160, 305, 341
ApoI RAATTY 1 cut(s) 823
Asp700I GAANNNNTTC 2 cut(s) 585, 618
AspLEI GCGC 1 cut(s) 642
AspS9I GGNCC 2 cut(s) 549, 550
AsuHPI GGTGA 3 cut(s) 767, 779, 920
AsuNHI GCTAGC 1 cut(s) 625
BaeGI GKGCMC 1 cut(s) 553
BanII GRGCYC 2 cut(s) 120, 553
BauI CACGAG 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 549
Bbv12I GWGCWC 2 cut(s) 120, 217
BbvI GCAGC 4 cut(s) 169, 172, 317, 328
BccI CCATC 2 cut(s) 854, 1133
BceAI ACGGC 1 cut(s) 512
BciT130I CCWGG 2 cut(s) 224, 310
BcoDI GTCTC 4 cut(s) 777, 849, 1047, 1202
BfaI CTAG 2 cut(s) 626, 728
BfmI CTRYAG 1 cut(s) 345
BfuAI ACCTGC 1 cut(s) 16
BisI GCNGC 5 cut(s) 158, 161, 306, 342, 702
BlsI GCNGC 5 cut(s) 159, 162, 307, 343, 703
Bme1390I CCNGG 2 cut(s) 224, 310
BmgT120I GGNCC 2 cut(s) 549, 550
BmiI GGNNCC 1 cut(s) 551
BmrFI CCNGG 2 cut(s) 224, 310
BmsI GCATC 3 cut(s) 228, 976, 1153
BmtI GCTAGC 1 cut(s) 629
BpiI GAAGAC 1 cut(s) 549
BpmI CTGGAG 1 cut(s) 759
BpuEI CTTGAG 4 cut(s) 494, 760, 821, 1110
BsaAI YACGTR 1 cut(s) 338
BsaBI GATNNNNATC 1 cut(s) 1143
BsaI GGTCTC 1 cut(s) 1047
BsaJI CCNNGG 1 cut(s) 111
Bse118I RCCGGY 1 cut(s) 1094
Bse1I ACTGG 1 cut(s) 435
Bse3DI GCAATG 1 cut(s) 100
Bse8I GATNNNNATC 1 cut(s) 1143
BseBI CCWGG 2 cut(s) 224, 310
BseDI CCNNGG 1 cut(s) 111
BseGI GGATG 2 cut(s) 107, 219
BseJI GATNNNNATC 1 cut(s) 1143
BseMI GCAATG 1 cut(s) 100
BseMII CTCAG 2 cut(s) 201, 377
BseNI ACTGG 1 cut(s) 435
BseRI GAGGAG 1 cut(s) 659
BseSI GKGCMC 1 cut(s) 553
BseXI GCAGC 4 cut(s) 169, 172, 317, 328
Bsh1236I CGCG 1 cut(s) 642
BshFI GGCC 3 cut(s) 99, 551, 806
BsiHKAI GWGCWC 2 cut(s) 120, 217
BsiSI CCGG 1 cut(s) 1095
BsmAI GTCTC 4 cut(s) 777, 849, 1047, 1202
BsmBI CGTCTC 1 cut(s) 849
BsmI GAATGC 1 cut(s) 163
BsnI GGCC 3 cut(s) 99, 551, 806
Bso31I GGTCTC 1 cut(s) 1047
Bsp120I GGGCCC 1 cut(s) 549
Bsp1286I GDGCHC 3 cut(s) 120, 217, 553
Bsp143I GATC 6 cut(s) 55, 208, 237, 743, 913, 1184
BspACI CCGC 1 cut(s) 701
BspANI GGCC 3 cut(s) 99, 551, 806
BspCNI CTCAG 2 cut(s) 200, 378
BspFNI CGCG 1 cut(s) 642
BspHI TCATGA 1 cut(s) 279
BspLI GGNNCC 1 cut(s) 551
BspMI ACCTGC 1 cut(s) 16
BspOI GCTAGC 1 cut(s) 629
BspPI GGATC 3 cut(s) 232, 738, 1192
BspQI GCTCTTC 1 cut(s) 147
BspTNI GGTCTC 1 cut(s) 1047
BsrDI GCAATG 1 cut(s) 100
BsrFI RCCGGY 1 cut(s) 1094
BsrI ACTGG 1 cut(s) 435
BssAI RCCGGY 1 cut(s) 1094
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 6 cut(s) 55, 208, 237, 743, 913, 1184
BssSI CACGAG 1 cut(s) 135
BssT1I CCWWGG 1 cut(s) 111
Bst2BI CACGAG 1 cut(s) 135
Bst2UI CCWGG 2 cut(s) 224, 310
Bst4CI ACNGT 1 cut(s) 405
Bst6I CTCTTC 4 cut(s) 147, 479, 588, 879
BstBAI YACGTR 1 cut(s) 338
BstC8I GCNNGC 3 cut(s) 217, 352, 627
BstDEI CTNAG 4 cut(s) 170, 187, 386, 1201
BstF5I GGATG 2 cut(s) 107, 219
BstFNI CGCG 1 cut(s) 642
BstHHI GCGC 1 cut(s) 642
BstKTI GATC 6 cut(s) 58, 211, 240, 746, 916, 1187
BstMAI GTCTC 4 cut(s) 777, 849, 1047, 1202
BstMBI GATC 6 cut(s) 55, 208, 237, 743, 913, 1184
BstMWI GCNNNNNNNGC 2 cut(s) 347, 356
BstNI CCWGG 2 cut(s) 224, 310
BstNSI RCATGY 2 cut(s) 354, 1136
BstSCI CCNGG 2 cut(s) 222, 308
BstSFI CTRYAG 1 cut(s) 345
BstSLI GKGCMC 1 cut(s) 553
BstUI CGCG 1 cut(s) 642
BstV1I GCAGC 4 cut(s) 169, 172, 317, 328
BstV2I GAAGAC 1 cut(s) 549
BstX2I RGATCY 2 cut(s) 743, 1184
BstYI RGATCY 2 cut(s) 743, 1184
BsuRI GGCC 3 cut(s) 99, 551, 806
BtsCI GGATG 2 cut(s) 107, 219
BtsI GCAGTG 1 cut(s) 769
BtsIMutI CAGTG 1 cut(s) 769
BveI ACCTGC 1 cut(s) 16
Cac8I GCNNGC 3 cut(s) 217, 352, 627
CciI TCATGA 1 cut(s) 279
CfoI GCGC 1 cut(s) 642
Cfr10I RCCGGY 1 cut(s) 1094
Cfr13I GGNCC 2 cut(s) 549, 550
Csp6I GTAC 2 cut(s) 274, 679
CspCI CAANNNNNGTGG 2 cut(s) 1081, 1116
CviAII CATG 4 cut(s) 280, 351, 937, 1133
CviQI GTAC 2 cut(s) 274, 679
DdeI CTNAG 4 cut(s) 170, 187, 386, 1201
DpnI GATC 6 cut(s) 57, 210, 239, 745, 915, 1186
DpnII GATC 6 cut(s) 55, 208, 237, 743, 913, 1184
DraI TTTAAA 1 cut(s) 1030
Eam1104I CTCTTC 4 cut(s) 147, 479, 588, 879
EarI CTCTTC 4 cut(s) 147, 479, 588, 879
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 111
Eco147I AGGCCT 1 cut(s) 806
Eco24I GRGCYC 2 cut(s) 120, 553
Eco31I GGTCTC 1 cut(s) 1047
Eco53kI GAGCTC 1 cut(s) 118
Eco57I CTGAAG 3 cut(s) 605, 737, 1011
EcoICRI GAGCTC 1 cut(s) 118
EcoO109I RGGNCCY 1 cut(s) 549
EcoRII CCWGG 2 cut(s) 222, 308
EcoT14I CCWWGG 1 cut(s) 111
EcoT22I ATGCAT 2 cut(s) 68, 356
EcoT38I GRGCYC 2 cut(s) 120, 553
ErhI CCWWGG 1 cut(s) 111
Esp3I CGTCTC 1 cut(s) 849
FaeI CATG 4 cut(s) 283, 354, 940, 1136
FatI CATG 4 cut(s) 279, 350, 936, 1132
FauNDI CATATG 2 cut(s) 43, 1073
Fnu4HI GCNGC 5 cut(s) 158, 161, 306, 342, 702
FokI GGATG 2 cut(s) 94, 206
FriOI GRGCYC 2 cut(s) 120, 553
Fsp4HI GCNGC 5 cut(s) 158, 161, 306, 342, 702
FspBI CTAG 2 cut(s) 626, 728
GlaI GCGC 1 cut(s) 641
GluI GCNGC 5 cut(s) 158, 161, 306, 342, 702
GsuI CTGGAG 1 cut(s) 759
HaeIII GGCC 3 cut(s) 99, 551, 806
HapII CCGG 1 cut(s) 1095
HhaI GCGC 1 cut(s) 642
Hin1II CATG 4 cut(s) 283, 354, 940, 1136
Hin6I GCGC 1 cut(s) 640
HinP1I GCGC 1 cut(s) 640
HindIII AAGCTT 1 cut(s) 416
HinfI GANTC 7 cut(s) 38, 575, 770, 789, 902, 998, 1220
HpaII CCGG 1 cut(s) 1095
HphI GGTGA 3 cut(s) 767, 779, 920
Hpy188I TCNGA 6 cut(s) 242, 580, 657, 670, 840, 1051
Hpy188III TCNNGA 6 cut(s) 149, 280, 590, 776, 1127, 1174
HpyAV CCTTC 4 cut(s) 76, 752, 845, 1032
HpyCH4III ACNGT 1 cut(s) 405
HpyCH4IV ACGT 1 cut(s) 337
HpyCH4V TGCA 7 cut(s) 25, 66, 261, 293, 354, 875, 1136
HpyF10VI GCNNNNNNNGC 2 cut(s) 347, 356
HpyF3I CTNAG 4 cut(s) 170, 187, 386, 1201
HpySE526I ACGT 1 cut(s) 337
Hsp92II CATG 4 cut(s) 283, 354, 940, 1136
HspAI GCGC 1 cut(s) 640
Kzo9I GATC 6 cut(s) 55, 208, 237, 743, 913, 1184
LguI GCTCTTC 1 cut(s) 147
LmnI GCTCC 2 cut(s) 115, 322
Lsp1109I GCAGC 4 cut(s) 169, 172, 317, 328
LweI GCATC 3 cut(s) 228, 976, 1153
MaeI CTAG 2 cut(s) 626, 728
MaeII ACGT 1 cut(s) 337
MalI GATC 6 cut(s) 57, 210, 239, 745, 915, 1186
MboI GATC 6 cut(s) 55, 208, 237, 743, 913, 1184
MfeI CAATTG 1 cut(s) 233
MflI RGATCY 2 cut(s) 743, 1184
MhlI GDGCHC 3 cut(s) 120, 217, 553
MluCI AATT 4 cut(s) 233, 267, 823, 1025
MlyI GAGTC 2 cut(s) 584, 779
MnlI CCTC 9 cut(s) 79, 243, 287, 471, 589, 603, 637, 724, 796
Mph1103I ATGCAT 2 cut(s) 68, 356
MroXI GAANNNNTTC 2 cut(s) 585, 618
MseI TTAA 4 cut(s) 69, 812, 1029, 1079
MslI CAYNNNNRTG 2 cut(s) 753, 1137
MspI CCGG 1 cut(s) 1095
MspR9I CCNGG 2 cut(s) 224, 310
MunI CAATTG 1 cut(s) 233
Mva1269I GAATGC 1 cut(s) 163
MvaI CCWGG 2 cut(s) 224, 310
MvnI CGCG 1 cut(s) 642
MwoI GCNNNNNNNGC 2 cut(s) 347, 356
NdeI CATATG 2 cut(s) 43, 1073
NdeII GATC 6 cut(s) 55, 208, 237, 743, 913, 1184
NheI GCTAGC 1 cut(s) 625
NlaIII CATG 4 cut(s) 283, 354, 940, 1136
NlaIV GGNNCC 1 cut(s) 551
NsiI ATGCAT 2 cut(s) 68, 356
NspI RCATGY 2 cut(s) 354, 1136
PaeI GCATGC 1 cut(s) 354
PagI TCATGA 1 cut(s) 279
PceI AGGCCT 1 cut(s) 806
PciSI GCTCTTC 1 cut(s) 147
PctI GAATGC 1 cut(s) 163
PdmI GAANNNNTTC 2 cut(s) 585, 618
PfeI GAWTC 5 cut(s) 38, 789, 902, 998, 1220
PkrI GCNGC 5 cut(s) 159, 162, 307, 343, 703
PleI GAGTC 2 cut(s) 583, 778
PpsI GAGTC 2 cut(s) 583, 778
Ppu21I YACGTR 1 cut(s) 338
Psp124BI GAGCTC 1 cut(s) 120
Psp6I CCWGG 2 cut(s) 222, 308
PspGI CCWGG 2 cut(s) 222, 308
PspN4I GGNNCC 1 cut(s) 551
PspOMI GGGCCC 1 cut(s) 549
PspPI GGNCC 2 cut(s) 549, 550
PsuI RGATCY 2 cut(s) 743, 1184
RsaI GTAC 2 cut(s) 275, 680
RsaNI GTAC 2 cut(s) 274, 679
RseI CAYNNNNRTG 2 cut(s) 753, 1137
SacI GAGCTC 1 cut(s) 120
SapI GCTCTTC 1 cut(s) 147
SaqAI TTAA 4 cut(s) 69, 812, 1029, 1079
SatI GCNGC 5 cut(s) 158, 161, 306, 342, 702
Sau3AI GATC 6 cut(s) 55, 208, 237, 743, 913, 1184
Sau96I GGNCC 2 cut(s) 549, 550
SchI GAGTC 2 cut(s) 584, 779
ScrFI CCNGG 2 cut(s) 224, 310
SduI GDGCHC 3 cut(s) 120, 217, 553
SfaNI GCATC 3 cut(s) 228, 976, 1153
SfcI CTRYAG 1 cut(s) 345
SmiMI CAYNNNNRTG 2 cut(s) 753, 1137
SmlI CTYRAG 4 cut(s) 473, 739, 800, 1125
SmoI CTYRAG 4 cut(s) 473, 739, 800, 1125
SphI GCATGC 1 cut(s) 354
Sse9I AATT 4 cut(s) 233, 267, 823, 1025
SseBI AGGCCT 1 cut(s) 806
SsiI CCGC 1 cut(s) 701
SspMI CTAG 2 cut(s) 626, 728
SstI GAGCTC 1 cut(s) 120
StuI AGGCCT 1 cut(s) 806
StyD4I CCNGG 2 cut(s) 222, 308
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 1 cut(s) 405
TaiI ACGT 1 cut(s) 340
TaqI TCGA 2 cut(s) 58, 211
TasI AATT 4 cut(s) 233, 267, 823, 1025
TauI GCSGC 1 cut(s) 704
TfiI GAWTC 5 cut(s) 38, 789, 902, 998, 1220
Tru1I TTAA 4 cut(s) 69, 812, 1029, 1079
Tru9I TTAA 4 cut(s) 69, 812, 1029, 1079
TscAI CASTG 1 cut(s) 769
TseI GCWGC 4 cut(s) 157, 160, 305, 341
TspDTI ATGAA 6 cut(s) 51, 218, 296, 674, 908, 909
TspRI CASTG 1 cut(s) 769
XapI RAATTY 1 cut(s) 823
XceI RCATGY 2 cut(s) 354, 1136
XmnI GAANNNNTTC 2 cut(s) 585, 618
XspI CTAG 2 cut(s) 626, 728
Zsp2I ATGCAT 2 cut(s) 68, 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.