Rmu_sc0005544.1_g000006

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005544.1
OG0014697
Physical Location & Seq
Forward (+)
33121 .. 35837
2717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005544.1_g000006.1.cds

Sequence Viewer

Length: 1743 bp
atgtctttgcagaaccgccacgacttgatcgagaggcaatgcagcaagatcaggaagcgaggctgttcgtcttcgtcctcgtcttccttggtgcgtaggtacaggttgaagcgggccattcttgtcgggaagcgagccgggtcgagtacgccggtgccaacttggaagacttcaacgagccccagatcgccgcccaattgcgccaccatgccgaagaagcagaagggtcagcagcattccaaggacgacgggaaagggaaggaagtcatgtcagtctcagcaagaaagctcgccgctacgttgtgggagatcaatgaagtccctgatgtgaaaagcaaagattctaaaatcaaagaggaagagggtattgctcaaatgccgccaaaaatcccagatccatcttattctcctatttcagagagaaaagctggatctggaggtgcacacgagagaagattgtcggtttctcagagaattcaaccgactgatcactacttgggagggtttgagactcatagcagtcacagtgtaattgaggtcaaagatgaatcatatggaaaaactgatcgaaaatgcattaagacctgtttgaaggaggtcagcaatggcctttctacatccaaggagctcgtaaaagttctaactcgtgtttctggtcttgaagagcagcagcattctctaagtatgcccctgctctcagccttacgagttgaacttgatcgagcgcgcgtccaggttcatcaattgatccgagaacagaggtcaaactgcaaagaaattgagtacctcatgaagcattttgcagaagaaaaggcagcctggaaaagaaaggagcgagagaaaatacgtgctgctgtagcatgcatagcagaagaacttgaagtagagaagaagctgagaaaacaaacagaacggttgaacaaaaagcttggcaaagaactggaagataaaaatgtagctctgtcaaaggcaacaaaagaacttgagagggagaagagagcaaaagagatttttgagcaagtttgtgatgagttagccgtaggtatgggggaagacagggcccaagttgaagaactaaagagagagtcggaaaaagttcgtgaagaggtggaaaaggagagggaaatgcttcagctagcagatgtgttgcgcgaggagagagttcagatgaagctctcggaagcaaagtaccattttgaagaaaaaaatgcggctgttgagcaactgaagaatgagctagagggctacttgagatcccataagggtgaaggcagtggtgagtctccagacttcaaaagaatcaaggaacttgaggcctacttaaagaaaataaattttgggtcatttcagagcataaaggaaggagacgatggaatggaagttgcatacagagaagagtgtgatgaagaagattcaggtgatgatcttcattccattgaactaaacatggacaatggtaataggagctacaagtggagttatgcttgtggagatgatgctgaagatgattcaaaaaggagttcagttgataaacaatttaaaggaagaaggtctctctctgaaaagatacaatgggaaaacatatgcttaaacaaaagttccaccggcattgattggggatttggctcaaaaactcaagaacatgcagatgggcatcaaacacaagaacacgaaactgaaatcaagaaaaatggatctgctgtatgtctcttagaccatatcttatatgattcaaaagtatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

580

Amino Acids

66.11

Weight (kDa)

8.08

Isoelectric Point (pI)

55.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014697)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g25790 FvH4_3g25790
malus_domestica MD03G1150600.v1.1 MD11G1170000.v1.1
prunus_persica Prupe.6G137500_v2.0.a1
pyrus_communis pycom03g10470 pycom11g14240
rosa_chinensis RchiOBHm_Chr5g0047011
rosa_laevigata RLG00000034458
rosa_multiflora Rmu_sc0005544.1_g000006
rosa_roxburghii Rroxscaffold_1G00034420
rosa_rugosa Rorug05G0233200
rosa_samantha Rh5AG312400 Rh5BG321500 Rh5CG347700 Rh5DG332400
rosa_wichuraiana Rw5G029200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 154
AccII CGCG 3 cut(s) 727, 729, 1152
AciI CCGC 6 cut(s) 16, 112, 191, 294, 380, 1211
AclWI GGATC 5 cut(s) 389, 439, 742, 1248, 1702
AcsI RAATTY 2 cut(s) 474, 1333
AcuI CTGAAG 3 cut(s) 1115, 1247, 1521
AfaI GTAC 4 cut(s) 101, 148, 785, 1190
AjnI CCWGG 2 cut(s) 732, 818
Alw21I GWGCWC 2 cut(s) 445, 630
Alw26I GTCTC 6 cut(s) 280, 503, 1287, 1359, 1557, 1712
Alw44I GTGCAC 1 cut(s) 441
AlwI GGATC 5 cut(s) 389, 439, 742, 1248, 1702
AoxI GGCC 4 cut(s) 114, 607, 1059, 1314
ApaI GGGCCC 1 cut(s) 1063
ApaLI GTGCAC 1 cut(s) 441
ApeKI GCWGC 6 cut(s) 42, 232, 667, 670, 815, 851
ApoI RAATTY 2 cut(s) 474, 1333
Asp700I GAANNNNTTC 2 cut(s) 1095, 1128
AspLEI GCGC 4 cut(s) 203, 727, 729, 1152
AspS9I GGNCC 3 cut(s) 114, 1059, 1060
AsuC2I CCSGG 1 cut(s) 139
AsuHPI GGTGA 3 cut(s) 1277, 1289, 1430
AsuNHI GCTAGC 1 cut(s) 1135
BaeGI GKGCMC 2 cut(s) 445, 1063
BanI GGYRCC 1 cut(s) 154
BanII GRGCYC 3 cut(s) 182, 630, 1063
BauI CACGAG 2 cut(s) 446, 645
BbsI GAAGAC 4 cut(s) 63, 75, 173, 1059
Bbv12I GWGCWC 2 cut(s) 445, 630
BbvI GCAGC 6 cut(s) 54, 244, 679, 682, 827, 838
BccI CCATC 3 cut(s) 406, 1364, 1643
BceAI ACGGC 1 cut(s) 1022
BciT130I CCWGG 2 cut(s) 734, 820
BclI TGATCA 1 cut(s) 487
BcnI CCSGG 1 cut(s) 139
BcoDI GTCTC 6 cut(s) 280, 503, 1287, 1359, 1557, 1712
BfaI CTAG 2 cut(s) 1136, 1238
BfmI CTRYAG 1 cut(s) 855
Bme1390I CCNGG 3 cut(s) 139, 734, 820
BmgT120I GGNCC 3 cut(s) 114, 1059, 1060
BmiI GGNNCC 2 cut(s) 156, 1061
BmrFI CCNGG 3 cut(s) 139, 734, 820
BmsI GCATC 2 cut(s) 1486, 1663
BmtI GCTAGC 1 cut(s) 1139
BpiI GAAGAC 4 cut(s) 63, 75, 173, 1059
BpmI CTGGAG 2 cut(s) 456, 1269
BpuEI CTTGAG 4 cut(s) 1004, 1270, 1331, 1620
BpuMI CCSGG 1 cut(s) 139
BsaAI YACGTR 1 cut(s) 848
BsaBI GATNNNNATC 1 cut(s) 1653
BsaI GGTCTC 1 cut(s) 1557
BsaJI CCNNGG 3 cut(s) 87, 240, 621
Bse118I RCCGGY 2 cut(s) 151, 1604
Bse1I ACTGG 1 cut(s) 945
Bse3DI GCAATG 2 cut(s) 44, 610
Bse8I GATNNNNATC 1 cut(s) 1653
BseBI CCWGG 2 cut(s) 734, 820
BseDI CCNNGG 3 cut(s) 87, 240, 621
BseGI GGATG 1 cut(s) 617
BseJI GATNNNNATC 1 cut(s) 1653
BseMI GCAATG 2 cut(s) 44, 610
BseMII CTCAG 4 cut(s) 291, 482, 711, 887
BseNI ACTGG 1 cut(s) 945
BsePI GCGCGC 1 cut(s) 725
BseRI GAGGAG 1 cut(s) 1169
BseSI GKGCMC 2 cut(s) 445, 1063
BseXI GCAGC 6 cut(s) 54, 244, 679, 682, 827, 838
Bsh1236I CGCG 3 cut(s) 727, 729, 1152
BshFI GGCC 4 cut(s) 116, 609, 1061, 1316
BshNI GGYRCC 1 cut(s) 154
BsiHKAI GWGCWC 2 cut(s) 445, 630
BsiSI CCGG 3 cut(s) 138, 152, 1605
BslFI GGGAC 1 cut(s) 305
BsmAI GTCTC 6 cut(s) 280, 503, 1287, 1359, 1557, 1712
BsmBI CGTCTC 1 cut(s) 1359
BsmFI GGGAC 1 cut(s) 305
BsmI GAATGC 2 cut(s) 235, 673
BsnI GGCC 4 cut(s) 116, 609, 1061, 1316
Bso31I GGTCTC 1 cut(s) 1557
Bsp120I GGGCCC 1 cut(s) 1059
Bsp1286I GDGCHC 4 cut(s) 182, 445, 630, 1063
BspACI CCGC 6 cut(s) 16, 112, 191, 294, 380, 1211
BspANI GGCC 4 cut(s) 116, 609, 1061, 1316
BspCNI CTCAG 4 cut(s) 290, 481, 710, 888
BspFNI CGCG 3 cut(s) 727, 729, 1152
BspHI TCATGA 1 cut(s) 789
BspLI GGNNCC 2 cut(s) 156, 1061
BspOI GCTAGC 1 cut(s) 1139
BspPI GGATC 5 cut(s) 389, 439, 742, 1248, 1702
BspQI GCTCTTC 1 cut(s) 657
BspT107I GGYRCC 1 cut(s) 154
BspTNI GGTCTC 1 cut(s) 1557
BsrDI GCAATG 2 cut(s) 44, 610
BsrFI RCCGGY 2 cut(s) 151, 1604
BsrI ACTGG 1 cut(s) 945
BssAI RCCGGY 2 cut(s) 151, 1604
BssECI CCNNGG 3 cut(s) 87, 240, 621
BssHII GCGCGC 1 cut(s) 725
BssSI CACGAG 2 cut(s) 446, 645
BssT1I CCWWGG 3 cut(s) 87, 240, 621
Bst2BI CACGAG 2 cut(s) 446, 645
Bst2UI CCWGG 2 cut(s) 734, 820
Bst4CI ACNGT 2 cut(s) 527, 915
Bst6I CTCTTC 5 cut(s) 354, 657, 989, 1098, 1389
BstBAI YACGTR 1 cut(s) 848
BstC8I GCNNGC 6 cut(s) 114, 135, 291, 727, 862, 1137
BstDEI CTNAG 6 cut(s) 277, 468, 680, 697, 896, 1711
BstF5I GGATG 1 cut(s) 617
BstFNI CGCG 3 cut(s) 727, 729, 1152
BstHHI GCGC 4 cut(s) 203, 727, 729, 1152
BstMAI GTCTC 6 cut(s) 280, 503, 1287, 1359, 1557, 1712
BstMWI GCNNNNNNNGC 3 cut(s) 217, 857, 866
BstNI CCWGG 2 cut(s) 734, 820
BstNSI RCATGY 2 cut(s) 864, 1646
BstSCI CCNGG 3 cut(s) 137, 732, 818
BstSFI CTRYAG 1 cut(s) 855
BstSLI GKGCMC 2 cut(s) 445, 1063
BstUI CGCG 3 cut(s) 727, 729, 1152
BstV1I GCAGC 6 cut(s) 54, 244, 679, 682, 827, 838
BstV2I GAAGAC 4 cut(s) 63, 75, 173, 1059
BstX2I RGATCY 4 cut(s) 394, 431, 1253, 1694
BstYI RGATCY 4 cut(s) 394, 431, 1253, 1694
BsuRI GGCC 4 cut(s) 116, 609, 1061, 1316
BtsCI GGATG 1 cut(s) 617
BtsI GCAGTG 1 cut(s) 1279
BtsIMutI CAGTG 2 cut(s) 532, 1279
Cac8I GCNNGC 6 cut(s) 114, 135, 291, 727, 862, 1137
CciI TCATGA 1 cut(s) 789
CfoI GCGC 4 cut(s) 203, 727, 729, 1152
Cfr10I RCCGGY 2 cut(s) 151, 1604
Cfr13I GGNCC 3 cut(s) 114, 1059, 1060
CseI GACGC 1 cut(s) 718
Csp6I GTAC 4 cut(s) 100, 147, 784, 1189
CspCI CAANNNNNGTGG 2 cut(s) 1591, 1626
CviAII CATG 6 cut(s) 208, 268, 790, 861, 1447, 1643
CviQI GTAC 4 cut(s) 100, 147, 784, 1189
DdeI CTNAG 6 cut(s) 277, 468, 680, 697, 896, 1711
DraI TTTAAA 1 cut(s) 1540
Eam1104I CTCTTC 5 cut(s) 354, 657, 989, 1098, 1389
EarI CTCTTC 5 cut(s) 354, 657, 989, 1098, 1389
Ecl136II GAGCTC 1 cut(s) 628
Eco130I CCWWGG 3 cut(s) 87, 240, 621
Eco147I AGGCCT 1 cut(s) 1316
Eco24I GRGCYC 3 cut(s) 182, 630, 1063
Eco31I GGTCTC 1 cut(s) 1557
Eco53kI GAGCTC 1 cut(s) 628
Eco57I CTGAAG 3 cut(s) 1115, 1247, 1521
EcoICRI GAGCTC 1 cut(s) 628
EcoO109I RGGNCCY 1 cut(s) 1059
EcoRI GAATTC 1 cut(s) 474
EcoRII CCWGG 2 cut(s) 732, 818
EcoT14I CCWWGG 3 cut(s) 87, 240, 621
EcoT22I ATGCAT 2 cut(s) 578, 866
EcoT38I GRGCYC 3 cut(s) 182, 630, 1063
ErhI CCWWGG 3 cut(s) 87, 240, 621
Esp3I CGTCTC 1 cut(s) 1359
FaeI CATG 6 cut(s) 211, 271, 793, 864, 1450, 1646
FaqI GGGAC 1 cut(s) 305
FatI CATG 6 cut(s) 207, 267, 789, 860, 1446, 1642
FauI CCCGC 1 cut(s) 105
FauNDI CATATG 2 cut(s) 553, 1583
FbaI TGATCA 1 cut(s) 487
FokI GGATG 1 cut(s) 604
FriOI GRGCYC 3 cut(s) 182, 630, 1063
FspBI CTAG 2 cut(s) 1136, 1238
GlaI GCGC 4 cut(s) 202, 726, 728, 1151
GsuI CTGGAG 2 cut(s) 456, 1269
HaeIII GGCC 4 cut(s) 116, 609, 1061, 1316
HapII CCGG 3 cut(s) 138, 152, 1605
HgaI GACGC 1 cut(s) 718
HhaI GCGC 4 cut(s) 203, 727, 729, 1152
Hin1II CATG 6 cut(s) 211, 271, 793, 864, 1450, 1646
Hin6I GCGC 4 cut(s) 201, 725, 727, 1150
HinP1I GCGC 4 cut(s) 201, 725, 727, 1150
HindIII AAGCTT 1 cut(s) 926
HinfI GANTC 9 cut(s) 341, 511, 548, 1085, 1280, 1299, 1412, 1508, 1730
HpaII CCGG 3 cut(s) 138, 152, 1605
HphI GGTGA 3 cut(s) 1277, 1289, 1430
Hpy166II GTNNAC 1 cut(s) 443
Hpy188I TCNGA 8 cut(s) 418, 471, 752, 1090, 1167, 1180, 1350, 1561
Hpy8I GTNNAC 1 cut(s) 443
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 6 cut(s) 217, 253, 586, 1262, 1355, 1542
HpyCH4III ACNGT 2 cut(s) 527, 915
HpyCH4IV ACGT 2 cut(s) 299, 847
HpyCH4V TGCA 9 cut(s) 10, 42, 443, 576, 771, 803, 864, 1385, 1646
HpyF10VI GCNNNNNNNGC 3 cut(s) 217, 857, 866
HpyF3I CTNAG 6 cut(s) 277, 468, 680, 697, 896, 1711
HpySE526I ACGT 2 cut(s) 299, 847
Hsp92II CATG 6 cut(s) 211, 271, 793, 864, 1450, 1646
HspAI GCGC 4 cut(s) 201, 725, 727, 1150
Ksp22I TGATCA 1 cut(s) 487
LguI GCTCTTC 1 cut(s) 657
LmnI GCTCC 3 cut(s) 625, 832, 1464
Lsp1109I GCAGC 6 cut(s) 54, 244, 679, 682, 827, 838
LweI GCATC 2 cut(s) 1486, 1663
MaeI CTAG 2 cut(s) 1136, 1238
MaeII ACGT 2 cut(s) 299, 847
MaeIII GTNAC 1 cut(s) 521
MfeI CAATTG 2 cut(s) 196, 743
MflI RGATCY 4 cut(s) 394, 431, 1253, 1694
MhlI GDGCHC 4 cut(s) 182, 445, 630, 1063
MluCI AATT 7 cut(s) 196, 474, 531, 743, 777, 1333, 1535
MlyI GAGTC 3 cut(s) 505, 1094, 1289
MmeI TCCRAC 1 cut(s) 1068
Mph1103I ATGCAT 2 cut(s) 578, 866
MroXI GAANNNNTTC 2 cut(s) 1095, 1128
MseI TTAA 4 cut(s) 579, 1322, 1539, 1589
MslI CAYNNNNRTG 2 cut(s) 1263, 1647
MspI CCGG 3 cut(s) 138, 152, 1605
MspR9I CCNGG 3 cut(s) 139, 734, 820
MunI CAATTG 2 cut(s) 196, 743
Mva1269I GAATGC 2 cut(s) 235, 673
MvaI CCWGG 2 cut(s) 734, 820
MvnI CGCG 3 cut(s) 727, 729, 1152
MwoI GCNNNNNNNGC 3 cut(s) 217, 857, 866
NciI CCSGG 1 cut(s) 139
NdeI CATATG 2 cut(s) 553, 1583
NheI GCTAGC 1 cut(s) 1135
NlaIII CATG 6 cut(s) 211, 271, 793, 864, 1450, 1646
NlaIV GGNNCC 2 cut(s) 156, 1061
NmuCI GTSAC 1 cut(s) 521
NsiI ATGCAT 2 cut(s) 578, 866
NspI RCATGY 2 cut(s) 864, 1646
PaeI GCATGC 1 cut(s) 864
PagI TCATGA 1 cut(s) 789
PauI GCGCGC 1 cut(s) 725
PceI AGGCCT 1 cut(s) 1316
PciSI GCTCTTC 1 cut(s) 657
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PctI GAATGC 2 cut(s) 235, 673
PdmI GAANNNNTTC 2 cut(s) 1095, 1128
PfeI GAWTC 6 cut(s) 341, 548, 1299, 1412, 1508, 1730
PleI GAGTC 3 cut(s) 505, 1093, 1288
PpsI GAGTC 3 cut(s) 505, 1093, 1288
Ppu21I YACGTR 1 cut(s) 848
Psp124BI GAGCTC 1 cut(s) 630
Psp6I CCWGG 2 cut(s) 732, 818
PspGI CCWGG 2 cut(s) 732, 818
PspN4I GGNNCC 2 cut(s) 156, 1061
PspOMI GGGCCC 1 cut(s) 1059
PspPI GGNCC 3 cut(s) 114, 1059, 1060
PsuI RGATCY 4 cut(s) 394, 431, 1253, 1694
PteI GCGCGC 1 cut(s) 725
RsaI GTAC 4 cut(s) 101, 148, 785, 1190
RsaNI GTAC 4 cut(s) 100, 147, 784, 1189
RseI CAYNNNNRTG 2 cut(s) 1263, 1647
SacI GAGCTC 1 cut(s) 630
SapI GCTCTTC 1 cut(s) 657
SaqAI TTAA 4 cut(s) 579, 1322, 1539, 1589
Sau96I GGNCC 3 cut(s) 114, 1059, 1060
SchI GAGTC 3 cut(s) 505, 1094, 1289
ScrFI CCNGG 3 cut(s) 139, 734, 820
SduI GDGCHC 4 cut(s) 182, 445, 630, 1063
SfaNI GCATC 2 cut(s) 1486, 1663
SfcI CTRYAG 1 cut(s) 855
SgrAI CRCCGGYG 1 cut(s) 151
SmiMI CAYNNNNRTG 2 cut(s) 1263, 1647
SmlI CTYRAG 4 cut(s) 983, 1249, 1310, 1635
SmoI CTYRAG 4 cut(s) 983, 1249, 1310, 1635
SphI GCATGC 1 cut(s) 864
Sse9I AATT 7 cut(s) 196, 474, 531, 743, 777, 1333, 1535
SseBI AGGCCT 1 cut(s) 1316
SsiI CCGC 6 cut(s) 16, 112, 191, 294, 380, 1211
SspMI CTAG 2 cut(s) 1136, 1238
SstI GAGCTC 1 cut(s) 630
StuI AGGCCT 1 cut(s) 1316
StyD4I CCNGG 3 cut(s) 137, 732, 818
StyI CCWWGG 3 cut(s) 87, 240, 621
TaaI ACNGT 2 cut(s) 527, 915
TaiI ACGT 2 cut(s) 302, 850
TaqI TCGA 4 cut(s) 30, 143, 568, 721
TasI AATT 7 cut(s) 196, 474, 531, 743, 777, 1333, 1535
TauI GCSGC 4 cut(s) 193, 296, 382, 1214
TfiI GAWTC 6 cut(s) 341, 548, 1299, 1412, 1508, 1730
Tru1I TTAA 4 cut(s) 579, 1322, 1539, 1589
Tru9I TTAA 4 cut(s) 579, 1322, 1539, 1589
TscAI CASTG 2 cut(s) 532, 1279
TseFI GTSAC 1 cut(s) 521
TseI GCWGC 6 cut(s) 42, 232, 667, 670, 815, 851
Tsp45I GTSAC 1 cut(s) 521
TspDTI ATGAA 7 cut(s) 330, 561, 728, 806, 1184, 1418, 1419
TspRI CASTG 2 cut(s) 532, 1279
VneI GTGCAC 1 cut(s) 441
XapI RAATTY 2 cut(s) 474, 1333
XceI RCATGY 2 cut(s) 864, 1646
XmnI GAANNNNTTC 2 cut(s) 1095, 1128
XspI CTAG 2 cut(s) 1136, 1238
Zsp2I ATGCAT 2 cut(s) 578, 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.