MD03G1150600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014697
Physical Location & Seq
Forward (+)
16841736 .. 16844248
2513 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1150600.v1.1.491

Sequence Viewer

Length: 1386 bp
ATGGGAGGCTTCGAGAGACCTACAAGTGCTAGTTTAACTGAGGTCAAATATCAATCCTGTGGGAAACCTGATCGAAAATGCATACACGGATTTAAGACCGGTTTGAAGGATGTCAGTAGTGGCCTTTCTACATCTAAAGAGCTTGTGAAGGTTCTTACTCGCATTTCGGGTCTTGAAGAGCAGCATTCGTTAACTACAACCCTACTCTCAGCCTTACGAGTTGAGCTTGATCGAGCGCGTGTCCAGGTTCATCAACTGATCCGAGAACAGAGGTCTAACTGCAACGAAATTGAGTTTCTCATGAAGAAGTTCGCAGAAGAAAAGGCGGCCTGGAAAAGTAAGGAGCGAGAGAGGATACGTGCTGCAATAGCGTGCATGGCAGAAGAACTTGAGGTGGAGAAGAAGCTAAGAAGACAAACAGAAAGGTTGAACAAGAAGCTAGGGAAAGAACTGGCAGATAAAGAGGCAGCTCTGTCGAAGGCCACGAAAGAGCTCGAGAGGGAGAAGAGGGCAAAAGAGATTTTTGAGCAAGTTTGTGATGAGTTAGCCACAGGTCTTGGGGAGGACAGAGCACAAGTCGAAGAGCTAAAGAAAGAATCGGAGAAAGTCCGTGAAGAAGTGGAGAAGGAGAGGGAAATGCTTCAGCTAGCCGATGTGTTGCGCGAGGAGAGAGTCCAGATGAAGCTTTCTGAAGCCAAATATCATTTTGAGGAGAAAAATGCCGTTGTGGAACAGCTAAAGAATGAACTAGAGGCTCATTTGAGATCCAAAACGCGCGAAGGTAGTCGCGGTTCTCCAGATTTCAGTAAAATCAAGGAACTCGAGGCTTATTTAAAGAAGATAAATTTTGGATCATTTCAGAACGCGAAGATAGAAGGGAATGAAATGGAAGTTGCTGCATACAGAGTGGAATGTGAAGACGATTCAAAACGGACTTCAGTTGATAGCGATCTTCATTCCATCGAGCTAAGCATGGACAATAACAACAAGAGCTACAAATGGAGTTACGGTTGTGGAGATGATACTGAAGATGATTCAAAGCGGACTTCGGTTGATAAACAATTCAAAGGGAGAAGATCGCTCTCCGAAAAAATACAATGGGAAAGCATATGCTTAAACAAAAATTCCAGCGGTATCGATTGGGAGTTTGGCGTAAAATCTCAATGGCAGTCAGACGGGCGGCTTATAGAAATATCAGAGCTTGTTCCGCAAACTCAAACCCCAGAACATGAAACCGAAACAAAGAAAAATGGTTCTGTTAAGGGTCTTGCAGACCATAGGTTATCGAATTCAAAAGTGGTTCCGATCCATAGCCTAGCCGGTACAACTTGTCAGTTCCAATCCTTTCCGACGAAGGATCCAGGAAACGAAGTATGCGGAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

462

Amino Acids

52.56

Weight (kDa)

6.26

Isoelectric Point (pI)

42.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014697)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g25790 FvH4_3g25790
malus_domestica MD03G1150600.v1.1 MD11G1170000.v1.1
prunus_persica Prupe.6G137500_v2.0.a1
pyrus_communis pycom03g10470 pycom11g14240
rosa_chinensis RchiOBHm_Chr5g0047011
rosa_laevigata RLG00000034458
rosa_multiflora Rmu_sc0005544.1_g000006
rosa_roxburghii Rroxscaffold_1G00034420
rosa_rugosa Rorug05G0233200
rosa_samantha Rh5AG312400 Rh5BG321500 Rh5CG347700 Rh5DG332400
rosa_wichuraiana Rw5G029200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 6 cut(s) 238, 663, 775, 777, 789, 866
AciI CCGC 7 cut(s) 326, 789, 1042, 1131, 1180, 1208, 1377
AclWI GGATC 6 cut(s) 253, 759, 859, 1300, 1352, 1365
AcsI RAATTY 3 cut(s) 844, 1123, 1288
AcuI CTGAAG 4 cut(s) 626, 711, 921, 1047
AfaI GTAC 1 cut(s) 1324
AgeI ACCGGT 1 cut(s) 98
AgsI TTSAA 7 cut(s) 106, 176, 430, 927, 1038, 1066, 1293
AjnI CCWGG 3 cut(s) 243, 329, 1360
Alw21I GWGCWC 2 cut(s) 495, 574
Alw26I GTCTC 1 cut(s) 10
AlwI GGATC 6 cut(s) 253, 759, 859, 1300, 1352, 1365
Ama87I CYCGRG 2 cut(s) 494, 821
AoxI GGCC 3 cut(s) 121, 327, 480
ApeKI GCWGC 4 cut(s) 181, 362, 467, 896
ApoI RAATTY 3 cut(s) 844, 1123, 1288
AsiGI ACCGGT 1 cut(s) 98
Asp700I GAANNNNTTC 2 cut(s) 308, 639
AspLEI GCGC 3 cut(s) 238, 663, 777
AsuNHI GCTAGC 1 cut(s) 646
AvaI CYCGRG 2 cut(s) 494, 821
BamHI GGATCC 1 cut(s) 1357
BanII GRGCYC 1 cut(s) 495
BbsI GAAGAC 2 cut(s) 418, 924
Bbv12I GWGCWC 2 cut(s) 495, 574
BbvI GCAGC 4 cut(s) 193, 349, 479, 883
BccI CCATC 1 cut(s) 968
BceAI ACGGC 1 cut(s) 707
BcgI CGANNNNNNTGC 2 cut(s) 456, 490
BciT130I CCWGG 3 cut(s) 245, 331, 1362
BciVI GTATCC 1 cut(s) 348
BcoDI GTCTC 1 cut(s) 10
BfaI CTAG 5 cut(s) 30, 440, 647, 749, 1316
BfuI GTATCC 1 cut(s) 348
BisI GCNGC 6 cut(s) 182, 327, 363, 468, 897, 1181
BlpI GCTNAGC 1 cut(s) 968
BlsI GCNGC 6 cut(s) 183, 328, 364, 469, 898, 1182
Bme1390I CCNGG 3 cut(s) 245, 331, 1362
BmeT110I CYCGRG 2 cut(s) 494, 821
BmiI GGNNCC 2 cut(s) 1302, 1359
BmrFI CCNGG 3 cut(s) 245, 331, 1362
BmtI GCTAGC 1 cut(s) 650
BpiI GAAGAC 2 cut(s) 418, 924
BpmI CTGGAG 1 cut(s) 780
Bpu1102I GCTNAGC 1 cut(s) 968
BpuEI CTTGAG 1 cut(s) 410
Bsa29I ATCGAT 1 cut(s) 1137
BsaAI YACGTR 1 cut(s) 359
BsaBI GATNNNNATC 1 cut(s) 948
BsaI GGTCTC 1 cut(s) 10
BsaWI WCCGGW 1 cut(s) 98
BsaXI ACNNNNNCTCC 2 cut(s) 994, 1024
Bse118I RCCGGY 2 cut(s) 98, 1319
Bse1I ACTGG 1 cut(s) 456
Bse8I GATNNNNATC 1 cut(s) 948
BseBI CCWGG 3 cut(s) 245, 331, 1362
BseCI ATCGAT 1 cut(s) 1137
BseGI GGATG 1 cut(s) 115
BseJI GATNNNNATC 1 cut(s) 948
BseMII CTCAG 2 cut(s) 30, 222
BseNI ACTGG 1 cut(s) 456
BseRI GAGGAG 2 cut(s) 680, 725
BseXI GCAGC 4 cut(s) 193, 349, 479, 883
Bsh1236I CGCG 6 cut(s) 238, 663, 775, 777, 789, 866
BshFI GGCC 3 cut(s) 123, 329, 482
BshTI ACCGGT 1 cut(s) 98
BshVI ATCGAT 1 cut(s) 1137
BsiHKAI GWGCWC 2 cut(s) 495, 574
BsiHKCI CYCGRG 2 cut(s) 494, 821
BsiSI CCGG 2 cut(s) 99, 1320
BsmAI GTCTC 1 cut(s) 10
BsmI GAATGC 1 cut(s) 184
BsnI GGCC 3 cut(s) 123, 329, 482
Bso31I GGTCTC 1 cut(s) 10
BsoBI CYCGRG 2 cut(s) 494, 821
Bsp1286I GDGCHC 2 cut(s) 495, 574
Bsp143I GATC 9 cut(s) 70, 229, 258, 764, 851, 949, 1076, 1305, 1357
Bsp1720I GCTNAGC 1 cut(s) 968
BspACI CCGC 7 cut(s) 326, 789, 1042, 1131, 1180, 1208, 1377
BspANI GGCC 3 cut(s) 123, 329, 482
BspCNI CTCAG 2 cut(s) 31, 221
BspDI ATCGAT 1 cut(s) 1137
BspFNI CGCG 6 cut(s) 238, 663, 775, 777, 789, 866
BspHI TCATGA 1 cut(s) 300
BspLI GGNNCC 2 cut(s) 1302, 1359
BspOI GCTAGC 1 cut(s) 650
BspPI GGATC 6 cut(s) 253, 759, 859, 1300, 1352, 1365
BspQI GCTCTTC 2 cut(s) 171, 576
BspTNI GGTCTC 1 cut(s) 10
BsrFI RCCGGY 2 cut(s) 98, 1319
BsrI ACTGG 1 cut(s) 456
BssAI RCCGGY 2 cut(s) 98, 1319
BssMI GATC 9 cut(s) 70, 229, 258, 764, 851, 949, 1076, 1305, 1357
Bst2UI CCWGG 3 cut(s) 245, 331, 1362
Bst4CI ACNGT 1 cut(s) 1010
Bst6I CTCTTC 3 cut(s) 171, 500, 576
BstBAI YACGTR 1 cut(s) 359
BstC8I GCNNGC 2 cut(s) 373, 648
BstDEI CTNAG 4 cut(s) 39, 208, 407, 968
BstF5I GGATG 1 cut(s) 115
BstFNI CGCG 6 cut(s) 238, 663, 775, 777, 789, 866
BstHHI GCGC 3 cut(s) 238, 663, 777
BstKTI GATC 9 cut(s) 73, 232, 261, 767, 854, 952, 1079, 1308, 1360
BstMAI GTCTC 1 cut(s) 10
BstMBI GATC 9 cut(s) 70, 229, 258, 764, 851, 949, 1076, 1305, 1357
BstMWI GCNNNNNNNGC 3 cut(s) 368, 377, 1207
BstNI CCWGG 3 cut(s) 245, 331, 1362
BstSCI CCNGG 3 cut(s) 243, 329, 1360
BstUI CGCG 6 cut(s) 238, 663, 775, 777, 789, 866
BstV1I GCAGC 4 cut(s) 193, 349, 479, 883
BstV2I GAAGAC 2 cut(s) 418, 924
BstX2I RGATCY 2 cut(s) 764, 1357
BstYI RGATCY 2 cut(s) 764, 1357
Bsu15I ATCGAT 1 cut(s) 1137
BsuI GTATCC 1 cut(s) 348
BsuRI GGCC 3 cut(s) 123, 329, 482
BsuTUI ATCGAT 1 cut(s) 1137
BtsCI GGATG 1 cut(s) 115
Cac8I GCNNGC 2 cut(s) 373, 648
CciI TCATGA 1 cut(s) 300
CfoI GCGC 3 cut(s) 238, 663, 777
Cfr10I RCCGGY 2 cut(s) 98, 1319
ClaI ATCGAT 1 cut(s) 1137
Csp6I GTAC 1 cut(s) 1323
CspAI ACCGGT 1 cut(s) 98
CspCI CAANNNNNGTGG 2 cut(s) 538, 573
CviAII CATG 4 cut(s) 301, 376, 973, 1229
CviQI GTAC 1 cut(s) 1323
DdeI CTNAG 4 cut(s) 39, 208, 407, 968
DpnI GATC 9 cut(s) 72, 231, 260, 766, 853, 951, 1078, 1307, 1359
DpnII GATC 9 cut(s) 70, 229, 258, 764, 851, 949, 1076, 1305, 1357
DraI TTTAAA 1 cut(s) 834
Eam1104I CTCTTC 3 cut(s) 171, 500, 576
EarI CTCTTC 3 cut(s) 171, 500, 576
Ecl136II GAGCTC 1 cut(s) 493
Eco24I GRGCYC 1 cut(s) 495
Eco31I GGTCTC 1 cut(s) 10
Eco53kI GAGCTC 1 cut(s) 493
Eco57I CTGAAG 4 cut(s) 626, 711, 921, 1047
Eco88I CYCGRG 2 cut(s) 494, 821
EcoICRI GAGCTC 1 cut(s) 493
EcoRI GAATTC 1 cut(s) 1288
EcoRII CCWGG 3 cut(s) 243, 329, 1360
EcoT22I ATGCAT 1 cut(s) 83
EcoT38I GRGCYC 1 cut(s) 495
FaeI CATG 4 cut(s) 304, 379, 976, 1232
FatI CATG 4 cut(s) 300, 375, 972, 1228
FauNDI CATATG 1 cut(s) 1109
Fnu4HI GCNGC 6 cut(s) 182, 327, 363, 468, 897, 1181
FokI GGATG 1 cut(s) 122
FriOI GRGCYC 1 cut(s) 495
Fsp4HI GCNGC 6 cut(s) 182, 327, 363, 468, 897, 1181
FspBI CTAG 5 cut(s) 30, 440, 647, 749, 1316
GlaI GCGC 3 cut(s) 237, 662, 776
GluI GCNGC 6 cut(s) 182, 327, 363, 468, 897, 1181
GsuI CTGGAG 1 cut(s) 780
HaeIII GGCC 3 cut(s) 123, 329, 482
HapII CCGG 2 cut(s) 99, 1320
HhaI GCGC 3 cut(s) 238, 663, 777
Hin1II CATG 4 cut(s) 304, 379, 976, 1232
Hin6I GCGC 3 cut(s) 236, 661, 775
HinP1I GCGC 3 cut(s) 236, 661, 775
HincII GTYRAC 1 cut(s) 192
HindII GTYRAC 1 cut(s) 192
HindIII AAGCTT 1 cut(s) 683
HinfI GANTC 4 cut(s) 596, 672, 923, 1034
HpaI GTTAAC 1 cut(s) 192
HpaII CCGG 2 cut(s) 99, 1320
Hpy166II GTNNAC 1 cut(s) 192
Hpy188I TCNGA 9 cut(s) 263, 601, 691, 861, 1087, 1174, 1198, 1305, 1350
Hpy188III TCNNGA 6 cut(s) 13, 173, 301, 496, 676, 797
Hpy8I GTNNAC 1 cut(s) 192
Hpy99I CGWCG 1 cut(s) 1354
HpyAV CCTTC 7 cut(s) 100, 142, 472, 619, 773, 869, 1348
HpyCH4III ACNGT 1 cut(s) 1010
HpyCH4IV ACGT 1 cut(s) 358
HpyCH4V TGCA 6 cut(s) 81, 282, 365, 375, 899, 1271
HpyF10VI GCNNNNNNNGC 3 cut(s) 368, 377, 1207
HpyF3I CTNAG 4 cut(s) 39, 208, 407, 968
HpySE526I ACGT 1 cut(s) 358
Hsp92II CATG 4 cut(s) 304, 379, 976, 1232
HspAI GCGC 3 cut(s) 236, 661, 775
KspAI GTTAAC 1 cut(s) 192
Kzo9I GATC 9 cut(s) 70, 229, 258, 764, 851, 949, 1076, 1305, 1357
LguI GCTCTTC 2 cut(s) 171, 576
LmnI GCTCC 1 cut(s) 343
Lsp1109I GCAGC 4 cut(s) 193, 349, 479, 883
MaeI CTAG 5 cut(s) 30, 440, 647, 749, 1316
MaeII ACGT 1 cut(s) 358
MaeIII GTNAC 1 cut(s) 1004
MalI GATC 9 cut(s) 72, 231, 260, 766, 853, 951, 1078, 1307, 1359
MboI GATC 9 cut(s) 70, 229, 258, 764, 851, 949, 1076, 1305, 1357
MflI RGATCY 2 cut(s) 764, 1357
MhlI GDGCHC 2 cut(s) 495, 574
MluCI AATT 5 cut(s) 288, 844, 1061, 1123, 1288
MlyI GAGTC 1 cut(s) 681
MmeI TCCRAC 1 cut(s) 1373
Mph1103I ATGCAT 1 cut(s) 83
MroXI GAANNNNTTC 2 cut(s) 308, 639
MseI TTAA 6 cut(s) 35, 93, 191, 833, 1115, 1260
MspA1I CMGCKG 1 cut(s) 1131
MspI CCGG 2 cut(s) 99, 1320
MspR9I CCNGG 3 cut(s) 245, 331, 1362
Mva1269I GAATGC 1 cut(s) 184
MvaI CCWGG 3 cut(s) 245, 331, 1362
MvnI CGCG 6 cut(s) 238, 663, 775, 777, 789, 866
MwoI GCNNNNNNNGC 3 cut(s) 368, 377, 1207
NdeI CATATG 1 cut(s) 1109
NdeII GATC 9 cut(s) 70, 229, 258, 764, 851, 949, 1076, 1305, 1357
NheI GCTAGC 1 cut(s) 646
NlaIII CATG 4 cut(s) 304, 379, 976, 1232
NlaIV GGNNCC 2 cut(s) 1302, 1359
NsiI ATGCAT 1 cut(s) 83
PaeR7I CTCGAG 2 cut(s) 494, 821
PagI TCATGA 1 cut(s) 300
PciSI GCTCTTC 2 cut(s) 171, 576
PcsI WCGNNNNNNNCGW 1 cut(s) 482
PctI GAATGC 1 cut(s) 184
PdmI GAANNNNTTC 2 cut(s) 308, 639
PfeI GAWTC 3 cut(s) 596, 923, 1034
PfoI TCCNGGA 1 cut(s) 1360
PinAI ACCGGT 1 cut(s) 98
PkrI GCNGC 6 cut(s) 183, 328, 364, 469, 898, 1182
PleI GAGTC 1 cut(s) 680
PpsI GAGTC 1 cut(s) 680
Ppu21I YACGTR 1 cut(s) 359
Psp124BI GAGCTC 1 cut(s) 495
Psp6I CCWGG 3 cut(s) 243, 329, 1360
PspGI CCWGG 3 cut(s) 243, 329, 1360
PspN4I GGNNCC 2 cut(s) 1302, 1359
PspXI VCTCGAGB 1 cut(s) 821
PsrI GAACNNNNNNTAC 2 cut(s) 775, 807
PsuI RGATCY 2 cut(s) 764, 1357
RsaI GTAC 1 cut(s) 1324
RsaNI GTAC 1 cut(s) 1323
SacI GAGCTC 1 cut(s) 495
SapI GCTCTTC 2 cut(s) 171, 576
SaqAI TTAA 6 cut(s) 35, 93, 191, 833, 1115, 1260
SatI GCNGC 6 cut(s) 182, 327, 363, 468, 897, 1181
Sau3AI GATC 9 cut(s) 70, 229, 258, 764, 851, 949, 1076, 1305, 1357
SchI GAGTC 1 cut(s) 681
ScrFI CCNGG 3 cut(s) 245, 331, 1362
SduI GDGCHC 2 cut(s) 495, 574
Sfr274I CTCGAG 2 cut(s) 494, 821
SlaI CTCGAG 2 cut(s) 494, 821
SmlI CTYRAG 3 cut(s) 389, 494, 821
SmoI CTYRAG 3 cut(s) 389, 494, 821
Sse9I AATT 5 cut(s) 288, 844, 1061, 1123, 1288
SsiI CCGC 7 cut(s) 326, 789, 1042, 1131, 1180, 1208, 1377
SspMI CTAG 5 cut(s) 30, 440, 647, 749, 1316
SstI GAGCTC 1 cut(s) 495
StyD4I CCNGG 3 cut(s) 243, 329, 1360
TaaI ACNGT 1 cut(s) 1010
TaiI ACGT 1 cut(s) 361
TasI AATT 5 cut(s) 288, 844, 1061, 1123, 1288
TauI GCSGC 2 cut(s) 329, 1183
TfiI GAWTC 3 cut(s) 596, 923, 1034
Tru1I TTAA 6 cut(s) 35, 93, 191, 833, 1115, 1260
Tru9I TTAA 6 cut(s) 35, 93, 191, 833, 1115, 1260
TseI GCWGC 4 cut(s) 181, 362, 467, 896
TspDTI ATGAA 7 cut(s) 239, 317, 695, 759, 897, 944, 1245
TspGWI ACGGA 3 cut(s) 102, 599, 946
XapI RAATTY 3 cut(s) 844, 1123, 1288
XhoI CTCGAG 2 cut(s) 494, 821
XmnI GAANNNNTTC 2 cut(s) 308, 639
XspI CTAG 5 cut(s) 30, 440, 647, 749, 1316
Zsp2I ATGCAT 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.