Rh5DG332400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0014697
Physical Location & Seq
Forward (+)
43843914 .. 43847721
3808 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG332400.1

Sequence Viewer

Length: 1266 bp
ATGTTAAAGCTACAGTTGATTGCAGAAGCGCACATGTTGATTTCATATCACAATATGCAGGTCAAAGATGAATCATATGGAAAAACTGATCGAAAATGCATTAAGACCTGTTTGAAGGAGGTCAGCAATGGCCTTTCTACATCCAAGGAGCTCGTAAAAGTTCTAACTCGTGTTTCTGGTCTTGAAGAGCAGCAGCATTCTCTAAGTATGCCCCTGCTCTCAGCCTTACGAGTTGAACTTGATCGAGCGCGCGTCCAGGTTCATCAATTGATCCGAGAACAGAGGTCAAACTGCAAAGAAATTGAGTACCTCATGAAGCATTTTGCAGAAGAAAAGGCAGCCTGGAAAAGAAAGGAGCGAGAGAAAATACGTGCTGCTGTAGCATGCATAGCAGAAGAACTTGAAGTAGAGAAGAAGCTGAGAAAACAAACAGAACGGTTGAACAAAAAGCTTGGCAAAGAACTGGAAGATAAAAATGTAGCTCTGTCAAAGGCAACAAAAGAACTTGAGAGGGAGAAGAGAGCAAAAGAGATTTTTGAGCAAGTTTGTGATGAGTTAGCCGTAGGTATGGGGGAAGACAGGGCCCAAGTTGAAGAACTAAAGAGAGAGTCGGAAAAAGTTCGTGAAGAGGTGGAAAAGGAGAGGGAAATGCTTCAGCTAGCAGATGTGTTGCGCGAGGAGAGAGTTCAGATGAAGCTCTCGGAAGCGAAGTACCATTTTGAAGAAAAAAATGCGGCTGTTGAGCAACTGAAGAATGAGCTAGAGGGCTACTTGAGATCCCATAAGGGTGAAGGCAGTGGCGAGTCTCCAGACTTCAAAAGAATCAAGGAACTTGAGGCCTACTTAAAGAAAATAAATTTTGGGTCATTTCAGAGCATAAAGGAAGGAGACGATGGAATGGAAGTTGCATACAGAGAAGAGTGTGATGAAGAAGATTCAGGTGATGATCTTCATTCCATTGAACTAAACATGGACAATGGTAATAGGAGCTACAAGTGGAGTTACGCTTGTGGAGATGATGCTGAAGATGATTCAAAAAGGAGTTCAGTTGATAAACAATTTAAAGGAAGAAGGTCTCTCTCTGAGAAGATACAATGGGAAAACATATGCTTAAACAAAAGTTCCACCGGCATTGATTGGGGATTTGGCTCAAAAACTCAAGAACATGCAGATGGGCATCAAACACAAGAACACGAAACTGAAATCAAGAAAAATGGATCTGCTGTATGTCTCTTAGACCATATCTTATATGATTCAAAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

421

Amino Acids

48.69

Weight (kDa)

5.68

Isoelectric Point (pI)

47.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014697)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g25790 FvH4_3g25790
malus_domestica MD03G1150600.v1.1 MD11G1170000.v1.1
prunus_persica Prupe.6G137500_v2.0.a1
pyrus_communis pycom03g10470 pycom11g14240
rosa_chinensis RchiOBHm_Chr5g0047011
rosa_laevigata RLG00000034458
rosa_multiflora Rmu_sc0005544.1_g000006
rosa_roxburghii Rroxscaffold_1G00034420
rosa_rugosa Rorug05G0233200
rosa_samantha Rh5AG312400 Rh5BG321500 Rh5CG347700 Rh5DG332400
rosa_wichuraiana Rw5G029200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 49
AccII CGCG 3 cut(s) 250, 252, 675
AciI CCGC 1 cut(s) 734
AclWI GGATC 3 cut(s) 265, 771, 1225
AcsI RAATTY 1 cut(s) 856
AcuI CTGAAG 3 cut(s) 638, 770, 1044
AfaI GTAC 2 cut(s) 308, 713
AflIII ACRYGT 1 cut(s) 33
AjnI CCWGG 2 cut(s) 255, 341
AluBI AGCT 9 cut(s) 10, 151, 418, 451, 482, 658, 697, 760, 990
AluI AGCT 9 cut(s) 10, 151, 418, 451, 482, 658, 697, 760, 990
Alw21I GWGCWC 1 cut(s) 153
Alw26I GTCTC 4 cut(s) 810, 882, 1080, 1235
AlwI GGATC 3 cut(s) 265, 771, 1225
AoxI GGCC 3 cut(s) 130, 582, 837
ApaI GGGCCC 1 cut(s) 586
ApeKI GCWGC 4 cut(s) 190, 193, 338, 374
ApoI RAATTY 1 cut(s) 856
Asp700I GAANNNNTTC 2 cut(s) 618, 651
AspLEI GCGC 4 cut(s) 31, 250, 252, 675
AspS9I GGNCC 2 cut(s) 582, 583
AsuHPI GGTGA 2 cut(s) 800, 953
AsuNHI GCTAGC 1 cut(s) 658
BaeGI GKGCMC 1 cut(s) 586
BanII GRGCYC 2 cut(s) 153, 586
BauI CACGAG 1 cut(s) 168
BbsI GAAGAC 1 cut(s) 582
Bbv12I GWGCWC 1 cut(s) 153
BbvI GCAGC 4 cut(s) 202, 205, 350, 361
BccI CCATC 2 cut(s) 887, 1166
BceAI ACGGC 1 cut(s) 545
BciT130I CCWGG 2 cut(s) 257, 343
BcoDI GTCTC 4 cut(s) 810, 882, 1080, 1235
BfaI CTAG 2 cut(s) 659, 761
BfmI CTRYAG 2 cut(s) 11, 378
BfuAI ACCTGC 1 cut(s) 49
BisI GCNGC 5 cut(s) 191, 194, 339, 375, 735
BlsI GCNGC 5 cut(s) 192, 195, 340, 376, 736
Bme1390I CCNGG 2 cut(s) 257, 343
BmgT120I GGNCC 2 cut(s) 582, 583
BmiI GGNNCC 1 cut(s) 584
BmrFI CCNGG 2 cut(s) 257, 343
BmsI GCATC 2 cut(s) 1009, 1186
BmtI GCTAGC 1 cut(s) 662
BpiI GAAGAC 1 cut(s) 582
BpmI CTGGAG 1 cut(s) 792
BpuEI CTTGAG 4 cut(s) 527, 793, 854, 1143
BsaAI YACGTR 1 cut(s) 371
BsaBI GATNNNNATC 1 cut(s) 1176
BsaI GGTCTC 1 cut(s) 1080
BsaJI CCNNGG 1 cut(s) 144
Bse118I RCCGGY 1 cut(s) 1127
Bse1I ACTGG 1 cut(s) 468
Bse3DI GCAATG 1 cut(s) 133
Bse8I GATNNNNATC 1 cut(s) 1176
BseBI CCWGG 2 cut(s) 257, 343
BseDI CCNNGG 1 cut(s) 144
BseGI GGATG 1 cut(s) 140
BseJI GATNNNNATC 1 cut(s) 1176
BseMI GCAATG 1 cut(s) 133
BseMII CTCAG 3 cut(s) 234, 410, 1074
BseNI ACTGG 1 cut(s) 468
BsePI GCGCGC 1 cut(s) 248
BseRI GAGGAG 1 cut(s) 692
BseSI GKGCMC 1 cut(s) 586
BseXI GCAGC 4 cut(s) 202, 205, 350, 361
Bsh1236I CGCG 3 cut(s) 250, 252, 675
BshFI GGCC 3 cut(s) 132, 584, 839
BsiHKAI GWGCWC 1 cut(s) 153
BsiSI CCGG 1 cut(s) 1128
BsmAI GTCTC 4 cut(s) 810, 882, 1080, 1235
BsmBI CGTCTC 1 cut(s) 882
BsmI GAATGC 1 cut(s) 196
BsnI GGCC 3 cut(s) 132, 584, 839
Bso31I GGTCTC 1 cut(s) 1080
Bsp120I GGGCCC 1 cut(s) 582
Bsp1286I GDGCHC 2 cut(s) 153, 586
Bsp143I GATC 6 cut(s) 88, 241, 270, 776, 946, 1217
BspACI CCGC 1 cut(s) 734
BspANI GGCC 3 cut(s) 132, 584, 839
BspCNI CTCAG 3 cut(s) 233, 411, 1075
BspFNI CGCG 3 cut(s) 250, 252, 675
BspHI TCATGA 1 cut(s) 312
BspLI GGNNCC 1 cut(s) 584
BspMI ACCTGC 1 cut(s) 49
BspOI GCTAGC 1 cut(s) 662
BspPI GGATC 3 cut(s) 265, 771, 1225
BspQI GCTCTTC 1 cut(s) 180
BspTNI GGTCTC 1 cut(s) 1080
BsrDI GCAATG 1 cut(s) 133
BsrFI RCCGGY 1 cut(s) 1127
BsrI ACTGG 1 cut(s) 468
BssAI RCCGGY 1 cut(s) 1127
BssECI CCNNGG 1 cut(s) 144
BssHII GCGCGC 1 cut(s) 248
BssMI GATC 6 cut(s) 88, 241, 270, 776, 946, 1217
BssSI CACGAG 1 cut(s) 168
BssT1I CCWWGG 1 cut(s) 144
Bst2BI CACGAG 1 cut(s) 168
Bst2UI CCWGG 2 cut(s) 257, 343
Bst4CI ACNGT 2 cut(s) 15, 438
Bst6I CTCTTC 4 cut(s) 180, 512, 621, 912
BstBAI YACGTR 1 cut(s) 371
BstC8I GCNNGC 3 cut(s) 250, 385, 660
BstDEI CTNAG 5 cut(s) 203, 220, 419, 1083, 1234
BstF5I GGATG 1 cut(s) 140
BstFNI CGCG 3 cut(s) 250, 252, 675
BstHHI GCGC 4 cut(s) 31, 250, 252, 675
BstKTI GATC 6 cut(s) 91, 244, 273, 779, 949, 1220
BstMAI GTCTC 4 cut(s) 810, 882, 1080, 1235
BstMBI GATC 6 cut(s) 88, 241, 270, 776, 946, 1217
BstMWI GCNNNNNNNGC 2 cut(s) 380, 389
BstNI CCWGG 2 cut(s) 257, 343
BstNSI RCATGY 3 cut(s) 37, 387, 1169
BstSCI CCNGG 2 cut(s) 255, 341
BstSFI CTRYAG 2 cut(s) 11, 378
BstSLI GKGCMC 1 cut(s) 586
BstUI CGCG 3 cut(s) 250, 252, 675
BstV1I GCAGC 4 cut(s) 202, 205, 350, 361
BstV2I GAAGAC 1 cut(s) 582
BstX2I RGATCY 2 cut(s) 776, 1217
BstYI RGATCY 2 cut(s) 776, 1217
BsuRI GGCC 3 cut(s) 132, 584, 839
BtsCI GGATG 1 cut(s) 140
BtsI GCAGTG 1 cut(s) 802
BtsIMutI CAGTG 1 cut(s) 802
BveI ACCTGC 1 cut(s) 49
Cac8I GCNNGC 3 cut(s) 250, 385, 660
CciI TCATGA 1 cut(s) 312
CfoI GCGC 4 cut(s) 31, 250, 252, 675
Cfr10I RCCGGY 1 cut(s) 1127
Cfr13I GGNCC 2 cut(s) 582, 583
CseI GACGC 1 cut(s) 241
Csp6I GTAC 2 cut(s) 307, 712
CspCI CAANNNNNGTGG 2 cut(s) 1114, 1149
CviAII CATG 5 cut(s) 34, 313, 384, 970, 1166
CviQI GTAC 2 cut(s) 307, 712
DdeI CTNAG 5 cut(s) 203, 220, 419, 1083, 1234
DpnI GATC 6 cut(s) 90, 243, 272, 778, 948, 1219
DpnII GATC 6 cut(s) 88, 241, 270, 776, 946, 1217
DraI TTTAAA 1 cut(s) 1063
Eam1104I CTCTTC 4 cut(s) 180, 512, 621, 912
EarI CTCTTC 4 cut(s) 180, 512, 621, 912
Ecl136II GAGCTC 1 cut(s) 151
Eco130I CCWWGG 1 cut(s) 144
Eco147I AGGCCT 1 cut(s) 839
Eco24I GRGCYC 2 cut(s) 153, 586
Eco31I GGTCTC 1 cut(s) 1080
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 3 cut(s) 638, 770, 1044
EcoICRI GAGCTC 1 cut(s) 151
EcoO109I RGGNCCY 1 cut(s) 582
EcoRII CCWGG 2 cut(s) 255, 341
EcoT14I CCWWGG 1 cut(s) 144
EcoT22I ATGCAT 2 cut(s) 101, 389
EcoT38I GRGCYC 2 cut(s) 153, 586
ErhI CCWWGG 1 cut(s) 144
Esp3I CGTCTC 1 cut(s) 882
FaeI CATG 5 cut(s) 37, 316, 387, 973, 1169
FatI CATG 5 cut(s) 33, 312, 383, 969, 1165
FauNDI CATATG 2 cut(s) 76, 1106
Fnu4HI GCNGC 5 cut(s) 191, 194, 339, 375, 735
FokI GGATG 1 cut(s) 127
FriOI GRGCYC 2 cut(s) 153, 586
Fsp4HI GCNGC 5 cut(s) 191, 194, 339, 375, 735
FspBI CTAG 2 cut(s) 659, 761
GlaI GCGC 4 cut(s) 30, 249, 251, 674
GluI GCNGC 5 cut(s) 191, 194, 339, 375, 735
GsuI CTGGAG 1 cut(s) 792
HaeIII GGCC 3 cut(s) 132, 584, 839
HapII CCGG 1 cut(s) 1128
HgaI GACGC 1 cut(s) 241
HhaI GCGC 4 cut(s) 31, 250, 252, 675
Hin1II CATG 5 cut(s) 37, 316, 387, 973, 1169
Hin6I GCGC 4 cut(s) 29, 248, 250, 673
HinP1I GCGC 4 cut(s) 29, 248, 250, 673
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 7 cut(s) 71, 608, 803, 822, 935, 1031, 1253
HpaII CCGG 1 cut(s) 1128
HphI GGTGA 2 cut(s) 800, 953
Hpy188I TCNGA 6 cut(s) 275, 613, 690, 703, 873, 1084
Hpy188III TCNNGA 6 cut(s) 182, 313, 623, 809, 1160, 1207
HpyAV CCTTC 4 cut(s) 109, 785, 878, 1065
HpyCH4III ACNGT 2 cut(s) 15, 438
HpyCH4IV ACGT 1 cut(s) 370
HpyCH4V TGCA 8 cut(s) 23, 58, 99, 294, 326, 387, 908, 1169
HpyF10VI GCNNNNNNNGC 2 cut(s) 380, 389
HpyF3I CTNAG 5 cut(s) 203, 220, 419, 1083, 1234
HpySE526I ACGT 1 cut(s) 370
Hsp92II CATG 5 cut(s) 37, 316, 387, 973, 1169
HspAI GCGC 4 cut(s) 29, 248, 250, 673
Kzo9I GATC 6 cut(s) 88, 241, 270, 776, 946, 1217
LguI GCTCTTC 1 cut(s) 180
LmnI GCTCC 3 cut(s) 148, 355, 987
Lsp1109I GCAGC 4 cut(s) 202, 205, 350, 361
LweI GCATC 2 cut(s) 1009, 1186
MaeI CTAG 2 cut(s) 659, 761
MaeII ACGT 1 cut(s) 370
MaeIII GTNAC 1 cut(s) 1001
MalI GATC 6 cut(s) 90, 243, 272, 778, 948, 1219
MboI GATC 6 cut(s) 88, 241, 270, 776, 946, 1217
MfeI CAATTG 1 cut(s) 266
MflI RGATCY 2 cut(s) 776, 1217
MhlI GDGCHC 2 cut(s) 153, 586
MluCI AATT 4 cut(s) 266, 300, 856, 1058
MlyI GAGTC 2 cut(s) 617, 812
MmeI TCCRAC 1 cut(s) 591
MnlI CCTC 9 cut(s) 112, 276, 320, 504, 622, 636, 670, 757, 829
Mph1103I ATGCAT 2 cut(s) 101, 389
MroXI GAANNNNTTC 2 cut(s) 618, 651
MseI TTAA 5 cut(s) 5, 102, 845, 1062, 1112
MslI CAYNNNNRTG 2 cut(s) 786, 1170
MspI CCGG 1 cut(s) 1128
MspR9I CCNGG 2 cut(s) 257, 343
MunI CAATTG 1 cut(s) 266
Mva1269I GAATGC 1 cut(s) 196
MvaI CCWGG 2 cut(s) 257, 343
MvnI CGCG 3 cut(s) 250, 252, 675
MwoI GCNNNNNNNGC 2 cut(s) 380, 389
NdeI CATATG 2 cut(s) 76, 1106
NdeII GATC 6 cut(s) 88, 241, 270, 776, 946, 1217
NheI GCTAGC 1 cut(s) 658
NlaIII CATG 5 cut(s) 37, 316, 387, 973, 1169
NlaIV GGNNCC 1 cut(s) 584
NsiI ATGCAT 2 cut(s) 101, 389
NspI RCATGY 3 cut(s) 37, 387, 1169
PaeI GCATGC 1 cut(s) 387
PagI TCATGA 1 cut(s) 312
PauI GCGCGC 1 cut(s) 248
PceI AGGCCT 1 cut(s) 839
PciI ACATGT 1 cut(s) 33
PciSI GCTCTTC 1 cut(s) 180
PctI GAATGC 1 cut(s) 196
PdmI GAANNNNTTC 2 cut(s) 618, 651
PfeI GAWTC 5 cut(s) 71, 822, 935, 1031, 1253
PkrI GCNGC 5 cut(s) 192, 195, 340, 376, 736
PleI GAGTC 2 cut(s) 616, 811
PpsI GAGTC 2 cut(s) 616, 811
Ppu21I YACGTR 1 cut(s) 371
PscI ACATGT 1 cut(s) 33
Psp124BI GAGCTC 1 cut(s) 153
Psp6I CCWGG 2 cut(s) 255, 341
PspGI CCWGG 2 cut(s) 255, 341
PspN4I GGNNCC 1 cut(s) 584
PspOMI GGGCCC 1 cut(s) 582
PspPI GGNCC 2 cut(s) 582, 583
PsuI RGATCY 2 cut(s) 776, 1217
PteI GCGCGC 1 cut(s) 248
RsaI GTAC 2 cut(s) 308, 713
RsaNI GTAC 2 cut(s) 307, 712
RseI CAYNNNNRTG 2 cut(s) 786, 1170
SacI GAGCTC 1 cut(s) 153
SapI GCTCTTC 1 cut(s) 180
SaqAI TTAA 5 cut(s) 5, 102, 845, 1062, 1112
SatI GCNGC 5 cut(s) 191, 194, 339, 375, 735
Sau3AI GATC 6 cut(s) 88, 241, 270, 776, 946, 1217
Sau96I GGNCC 2 cut(s) 582, 583
SchI GAGTC 2 cut(s) 617, 812
ScrFI CCNGG 2 cut(s) 257, 343
SduI GDGCHC 2 cut(s) 153, 586
SfaNI GCATC 2 cut(s) 1009, 1186
SfcI CTRYAG 2 cut(s) 11, 378
SmiMI CAYNNNNRTG 2 cut(s) 786, 1170
SmlI CTYRAG 4 cut(s) 506, 772, 833, 1158
SmoI CTYRAG 4 cut(s) 506, 772, 833, 1158
SphI GCATGC 1 cut(s) 387
Sse9I AATT 4 cut(s) 266, 300, 856, 1058
SseBI AGGCCT 1 cut(s) 839
SsiI CCGC 1 cut(s) 734
SspMI CTAG 2 cut(s) 659, 761
SstI GAGCTC 1 cut(s) 153
StuI AGGCCT 1 cut(s) 839
StyD4I CCNGG 2 cut(s) 255, 341
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 2 cut(s) 15, 438
TaiI ACGT 1 cut(s) 373
TaqI TCGA 2 cut(s) 91, 244
TasI AATT 4 cut(s) 266, 300, 856, 1058
TauI GCSGC 1 cut(s) 737
TfiI GAWTC 5 cut(s) 71, 822, 935, 1031, 1253
Tru1I TTAA 5 cut(s) 5, 102, 845, 1062, 1112
Tru9I TTAA 5 cut(s) 5, 102, 845, 1062, 1112
TscAI CASTG 1 cut(s) 802
TseI GCWGC 4 cut(s) 190, 193, 338, 374
TspDTI ATGAA 7 cut(s) 33, 84, 251, 329, 707, 941, 942
TspRI CASTG 1 cut(s) 802
XapI RAATTY 1 cut(s) 856
XceI RCATGY 3 cut(s) 37, 387, 1169
XmnI GAANNNNTTC 2 cut(s) 618, 651
XspI CTAG 2 cut(s) 659, 761
Zsp2I ATGCAT 2 cut(s) 101, 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.