RLG00000034458

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014697
Physical Location & Seq
Forward (+)
43012746 .. 43015486
2741 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034458

Sequence Viewer

Length: 1746 bp
ATGTCTTTGCAGAACCGCCACGACTTGATCGAGAAGCAATGCAGCAAGATCAGGAAGCGAGGCTGTTCGTCTTCGTCCTCGTCTTCCTTGGTGCGTAGGTACAGGTTGAAGCGGGCCATTCTCGTCGGGAAGCGAGCCGGGTCGAGTACGCCGGTGCCAACTTGGAAGACTTCAACGAGCCCCAGATCGCCGCCCAATTGCGCCACCATGCCGAAGAAGCAGAAGGGTCAGCAGCATTCCAAGGACGACGGGAAAGGGAAGGAAGTCATGTCAGTCTCAGCAAGAAAGCTCGCCGCTACGTTGTGGGAGATCAATGAAGTCCCTGATTTGAAAAGCGAAGATTCTAAAATCAAAGAGGAAGAGGGTATTGCTCAAATGCCGCCAAAATTCCCAGATCCATCTTATTCTCCTATTTCAGAGAGAAAAACTGGATCTGGAGGTGGACACGAGAGAAGATTGTCGGCGGTTTCTCAGAGAATTCAACCGACTGATCACTACTTGGGAGGGTTTGAGACTCATAGCAGTCACAGTGTAATTGAGGTCAAAGATGAATCATATGGAAAAACTGATCGAAAATGCATTAAGACCTGTTTGAAGGAGGTCAGCAATGGCCTTTCTACATCCAAGGAGCTCGTAAAAGTTCTAACTCGTGTTTCTGGTCTTGAAGAGCAGCAGCATTCTCTAAGTATGCCCTTGCTCTCAGCCTTACGAGTTGAACTTGATAGAGCGCGCGTCCAGGTTCATCAATTGATCCGAGAACAGAGGTCAAACTGCAAAGAAATTGAGTACCTCATGAAGCATTTTGCAGAAGAAAAGGCAGCCTGGAAAAAAAAGGAGCGAGAGAAAATACGTGCTGCTGTAGCATGCATAGCAGAAGAACTTGAAGTAGAGAAGAAGCTGAGAAAACAAACGGAACGGTTGAACAAAAAGCTTGGCAAAGAACTGGAAGATAAAAATGTAGCTCTGTCAAAGGCAATAAAAGAACTTGAGAGGGAGAAGAGAGCAAAAGAGATTTTTGAGCAAGTTTGTGATGAGTTAGCCGTAGGTATGGGGGAAGACAGGGCCCAAGTTGAAGAACTAAAGAGAGAGTCGGAAAAAGTTCGTGAAGAGGTGGAAAAGGAGAGGGAAATGCTTCAGCTAGCAGATGTGTTGCGCGAGGAGAGAGTTCAGATGAAGCTCTCGGAAGCGAAGTACCATTTTGAAGAAAAAAATGCGGCTGTTGAGCAACTGAAGAATGAGCTAGAGGGCTACTTGAGATCCCATAAGGGTGAAGGCAGTGGTGAGTCTCCAGACTTCAAAAGAATCAAGGAACTTGAGGCCTACTTAAAGAAAATAAATTTTGGGTCATTTCATAGCATAAAGGAAGGAGACGATGGAATGGAAGTTGCATACAGAGAAGAGTGTGATGAAGAAGATTCAGGTGATGATCTTCATTCCATTGAACTAAACATGGACAATGGTAATAGGAGCTACAAGTGGAGTTATGCTTGTGGAGATGATGCTGAAGATGATTCGAAAAGGAGTTCAGTTGAAAAACAATTTAAAGGAAGAAGGTCTCTCTCTGAAAAGATACAATGGGAAAACATATGCTTAAACAAAAGTTCCACCGGCATTGATTGGGGATTTGGCTCAAAAACTCAAGAACATGCAGATGGGCATCAAACACAAGAACAGGAAACTGAGATCAAGAAAAACGGATCTGCTGTATGTCTCTTAGACCATATCTTATATGATTCAAAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

582

Amino Acids

66.22

Weight (kDa)

7.56

Isoelectric Point (pI)

56.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014697)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g25790 FvH4_3g25790
malus_domestica MD03G1150600.v1.1 MD11G1170000.v1.1
prunus_persica Prupe.6G137500_v2.0.a1
pyrus_communis pycom03g10470 pycom11g14240
rosa_chinensis RchiOBHm_Chr5g0047011
rosa_laevigata RLG00000034458
rosa_multiflora Rmu_sc0005544.1_g000006
rosa_roxburghii Rroxscaffold_1G00034420
rosa_rugosa Rorug05G0233200
rosa_samantha Rh5AG312400 Rh5BG321500 Rh5CG347700 Rh5DG332400
rosa_wichuraiana Rw5G029200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 154
AccII CGCG 3 cut(s) 730, 732, 1155
AciI CCGC 7 cut(s) 16, 112, 191, 294, 380, 464, 1214
AclWI GGATC 5 cut(s) 389, 439, 745, 1251, 1705
AcsI RAATTY 3 cut(s) 386, 477, 1336
AcuI CTGAAG 3 cut(s) 1118, 1250, 1524
AfaI GTAC 4 cut(s) 101, 148, 788, 1193
AjnI CCWGG 2 cut(s) 735, 821
AluBI AGCT 9 cut(s) 289, 631, 898, 931, 962, 1138, 1177, 1240, 1470
AluI AGCT 9 cut(s) 289, 631, 898, 931, 962, 1138, 1177, 1240, 1470
Alw21I GWGCWC 1 cut(s) 633
Alw26I GTCTC 6 cut(s) 280, 506, 1290, 1362, 1560, 1715
AlwI GGATC 5 cut(s) 389, 439, 745, 1251, 1705
AoxI GGCC 4 cut(s) 114, 610, 1062, 1317
ApaI GGGCCC 1 cut(s) 1066
ApeKI GCWGC 6 cut(s) 42, 232, 670, 673, 818, 854
ApoI RAATTY 3 cut(s) 386, 477, 1336
Asp700I GAANNNNTTC 2 cut(s) 1098, 1131
AspLEI GCGC 4 cut(s) 203, 730, 732, 1155
AspS9I GGNCC 3 cut(s) 114, 1062, 1063
AsuC2I CCSGG 1 cut(s) 139
AsuHPI GGTGA 3 cut(s) 1280, 1292, 1433
AsuII TTCGAA 1 cut(s) 1514
AsuNHI GCTAGC 1 cut(s) 1138
BaeGI GKGCMC 1 cut(s) 1066
BanI GGYRCC 1 cut(s) 154
BanII GRGCYC 3 cut(s) 182, 633, 1066
BauI CACGAG 2 cut(s) 446, 648
BbsI GAAGAC 4 cut(s) 63, 75, 173, 1062
Bbv12I GWGCWC 1 cut(s) 633
BbvI GCAGC 6 cut(s) 54, 244, 682, 685, 830, 841
BccI CCATC 3 cut(s) 406, 1367, 1646
BceAI ACGGC 1 cut(s) 1025
BciT130I CCWGG 2 cut(s) 737, 823
BclI TGATCA 1 cut(s) 490
BcnI CCSGG 1 cut(s) 139
BcoDI GTCTC 6 cut(s) 280, 506, 1290, 1362, 1560, 1715
BfaI CTAG 2 cut(s) 1139, 1241
BfmI CTRYAG 1 cut(s) 858
Bme1390I CCNGG 3 cut(s) 139, 737, 823
BmgT120I GGNCC 3 cut(s) 114, 1062, 1063
BmiI GGNNCC 2 cut(s) 156, 1064
BmrFI CCNGG 3 cut(s) 139, 737, 823
BmsI GCATC 2 cut(s) 1489, 1666
BmtI GCTAGC 1 cut(s) 1142
BpiI GAAGAC 4 cut(s) 63, 75, 173, 1062
BpmI CTGGAG 2 cut(s) 456, 1272
Bpu14I TTCGAA 1 cut(s) 1514
BpuEI CTTGAG 4 cut(s) 1007, 1273, 1334, 1623
BpuMI CCSGG 1 cut(s) 139
BsaAI YACGTR 1 cut(s) 851
BsaBI GATNNNNATC 1 cut(s) 1656
BsaI GGTCTC 1 cut(s) 1560
BsaJI CCNNGG 3 cut(s) 87, 240, 624
Bse118I RCCGGY 2 cut(s) 151, 1607
Bse1I ACTGG 2 cut(s) 433, 948
Bse3DI GCAATG 2 cut(s) 44, 613
Bse8I GATNNNNATC 1 cut(s) 1656
BseBI CCWGG 2 cut(s) 737, 823
BseDI CCNNGG 3 cut(s) 87, 240, 624
BseGI GGATG 1 cut(s) 620
BseJI GATNNNNATC 1 cut(s) 1656
BseMI GCAATG 2 cut(s) 44, 613
BseMII CTCAG 5 cut(s) 291, 485, 714, 890, 1671
BseNI ACTGG 2 cut(s) 433, 948
BsePI GCGCGC 1 cut(s) 728
BseRI GAGGAG 1 cut(s) 1172
BseSI GKGCMC 1 cut(s) 1066
BseXI GCAGC 6 cut(s) 54, 244, 682, 685, 830, 841
Bsh1236I CGCG 3 cut(s) 730, 732, 1155
BshFI GGCC 4 cut(s) 116, 612, 1064, 1319
BshNI GGYRCC 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 633
BsiSI CCGG 3 cut(s) 138, 152, 1608
BslFI GGGAC 1 cut(s) 305
BsmAI GTCTC 6 cut(s) 280, 506, 1290, 1362, 1560, 1715
BsmBI CGTCTC 1 cut(s) 1362
BsmFI GGGAC 1 cut(s) 305
BsmI GAATGC 2 cut(s) 235, 676
BsnI GGCC 4 cut(s) 116, 612, 1064, 1319
Bso31I GGTCTC 1 cut(s) 1560
Bsp119I TTCGAA 1 cut(s) 1514
Bsp120I GGGCCC 1 cut(s) 1062
Bsp1286I GDGCHC 3 cut(s) 182, 633, 1066
BspACI CCGC 7 cut(s) 16, 112, 191, 294, 380, 464, 1214
BspANI GGCC 4 cut(s) 116, 612, 1064, 1319
BspCNI CTCAG 5 cut(s) 290, 484, 713, 891, 1672
BspFNI CGCG 3 cut(s) 730, 732, 1155
BspHI TCATGA 1 cut(s) 792
BspLI GGNNCC 2 cut(s) 156, 1064
BspOI GCTAGC 1 cut(s) 1142
BspPI GGATC 5 cut(s) 389, 439, 745, 1251, 1705
BspQI GCTCTTC 1 cut(s) 660
BspT104I TTCGAA 1 cut(s) 1514
BspT107I GGYRCC 1 cut(s) 154
BspTNI GGTCTC 1 cut(s) 1560
BsrDI GCAATG 2 cut(s) 44, 613
BsrFI RCCGGY 2 cut(s) 151, 1607
BsrI ACTGG 2 cut(s) 433, 948
BssAI RCCGGY 2 cut(s) 151, 1607
BssECI CCNNGG 3 cut(s) 87, 240, 624
BssHII GCGCGC 1 cut(s) 728
BssSI CACGAG 2 cut(s) 446, 648
BssT1I CCWWGG 3 cut(s) 87, 240, 624
Bst2BI CACGAG 2 cut(s) 446, 648
Bst2UI CCWGG 2 cut(s) 737, 823
Bst4CI ACNGT 2 cut(s) 530, 918
Bst6I CTCTTC 5 cut(s) 354, 660, 992, 1101, 1392
BstBAI YACGTR 1 cut(s) 851
BstBI TTCGAA 1 cut(s) 1514
BstC8I GCNNGC 6 cut(s) 114, 135, 291, 730, 865, 1140
BstDEI CTNAG 7 cut(s) 277, 471, 683, 700, 899, 1680, 1714
BstF5I GGATG 1 cut(s) 620
BstFNI CGCG 3 cut(s) 730, 732, 1155
BstHHI GCGC 4 cut(s) 203, 730, 732, 1155
BstMAI GTCTC 6 cut(s) 280, 506, 1290, 1362, 1560, 1715
BstMWI GCNNNNNNNGC 3 cut(s) 217, 860, 869
BstNI CCWGG 2 cut(s) 737, 823
BstNSI RCATGY 2 cut(s) 867, 1649
BstSCI CCNGG 3 cut(s) 137, 735, 821
BstSFI CTRYAG 1 cut(s) 858
BstSLI GKGCMC 1 cut(s) 1066
BstUI CGCG 3 cut(s) 730, 732, 1155
BstV1I GCAGC 6 cut(s) 54, 244, 682, 685, 830, 841
BstV2I GAAGAC 4 cut(s) 63, 75, 173, 1062
BstX2I RGATCY 4 cut(s) 394, 431, 1256, 1697
BstYI RGATCY 4 cut(s) 394, 431, 1256, 1697
BsuRI GGCC 4 cut(s) 116, 612, 1064, 1319
BtsCI GGATG 1 cut(s) 620
BtsI GCAGTG 1 cut(s) 1282
BtsIMutI CAGTG 2 cut(s) 535, 1282
Cac8I GCNNGC 6 cut(s) 114, 135, 291, 730, 865, 1140
CciI TCATGA 1 cut(s) 792
CfoI GCGC 4 cut(s) 203, 730, 732, 1155
Cfr10I RCCGGY 2 cut(s) 151, 1607
Cfr13I GGNCC 3 cut(s) 114, 1062, 1063
CseI GACGC 1 cut(s) 721
Csp6I GTAC 4 cut(s) 100, 147, 787, 1192
CspCI CAANNNNNGTGG 2 cut(s) 1594, 1629
CviAII CATG 6 cut(s) 208, 268, 793, 864, 1450, 1646
CviQI GTAC 4 cut(s) 100, 147, 787, 1192
DdeI CTNAG 7 cut(s) 277, 471, 683, 700, 899, 1680, 1714
DraI TTTAAA 1 cut(s) 1543
Eam1104I CTCTTC 5 cut(s) 354, 660, 992, 1101, 1392
EarI CTCTTC 5 cut(s) 354, 660, 992, 1101, 1392
Ecl136II GAGCTC 1 cut(s) 631
Eco130I CCWWGG 3 cut(s) 87, 240, 624
Eco147I AGGCCT 1 cut(s) 1319
Eco24I GRGCYC 3 cut(s) 182, 633, 1066
Eco31I GGTCTC 1 cut(s) 1560
Eco53kI GAGCTC 1 cut(s) 631
Eco57I CTGAAG 3 cut(s) 1118, 1250, 1524
EcoICRI GAGCTC 1 cut(s) 631
EcoO109I RGGNCCY 1 cut(s) 1062
EcoRI GAATTC 1 cut(s) 477
EcoRII CCWGG 2 cut(s) 735, 821
EcoT14I CCWWGG 3 cut(s) 87, 240, 624
EcoT22I ATGCAT 2 cut(s) 581, 869
EcoT38I GRGCYC 3 cut(s) 182, 633, 1066
ErhI CCWWGG 3 cut(s) 87, 240, 624
Esp3I CGTCTC 1 cut(s) 1362
FaeI CATG 6 cut(s) 211, 271, 796, 867, 1453, 1649
FaqI GGGAC 1 cut(s) 305
FatI CATG 6 cut(s) 207, 267, 792, 863, 1449, 1645
FauI CCCGC 1 cut(s) 105
FauNDI CATATG 2 cut(s) 556, 1586
FbaI TGATCA 1 cut(s) 490
FokI GGATG 1 cut(s) 607
FriOI GRGCYC 3 cut(s) 182, 633, 1066
FspBI CTAG 2 cut(s) 1139, 1241
GlaI GCGC 4 cut(s) 202, 729, 731, 1154
GsuI CTGGAG 2 cut(s) 456, 1272
HaeIII GGCC 4 cut(s) 116, 612, 1064, 1319
HapII CCGG 3 cut(s) 138, 152, 1608
HgaI GACGC 1 cut(s) 721
HhaI GCGC 4 cut(s) 203, 730, 732, 1155
Hin1II CATG 6 cut(s) 211, 271, 796, 867, 1453, 1649
Hin6I GCGC 4 cut(s) 201, 728, 730, 1153
HinP1I GCGC 4 cut(s) 201, 728, 730, 1153
HindIII AAGCTT 1 cut(s) 929
HinfI GANTC 9 cut(s) 341, 514, 551, 1088, 1283, 1302, 1415, 1511, 1733
HpaII CCGG 3 cut(s) 138, 152, 1608
HphI GGTGA 3 cut(s) 1280, 1292, 1433
Hpy166II GTNNAC 1 cut(s) 443
Hpy188I TCNGA 7 cut(s) 418, 474, 755, 1093, 1170, 1183, 1564
Hpy8I GTNNAC 1 cut(s) 443
Hpy99I CGWCG 2 cut(s) 128, 251
HpyAV CCTTC 6 cut(s) 217, 253, 589, 1265, 1358, 1545
HpyCH4III ACNGT 2 cut(s) 530, 918
HpyCH4IV ACGT 2 cut(s) 299, 850
HpyCH4V TGCA 8 cut(s) 10, 42, 579, 774, 806, 867, 1388, 1649
HpyF10VI GCNNNNNNNGC 3 cut(s) 217, 860, 869
HpyF3I CTNAG 7 cut(s) 277, 471, 683, 700, 899, 1680, 1714
HpySE526I ACGT 2 cut(s) 299, 850
Hsp92II CATG 6 cut(s) 211, 271, 796, 867, 1453, 1649
HspAI GCGC 4 cut(s) 201, 728, 730, 1153
Ksp22I TGATCA 1 cut(s) 490
LguI GCTCTTC 1 cut(s) 660
LmnI GCTCC 3 cut(s) 628, 835, 1467
Lsp1109I GCAGC 6 cut(s) 54, 244, 682, 685, 830, 841
LweI GCATC 2 cut(s) 1489, 1666
MaeI CTAG 2 cut(s) 1139, 1241
MaeII ACGT 2 cut(s) 299, 850
MaeIII GTNAC 1 cut(s) 524
MfeI CAATTG 2 cut(s) 196, 746
MflI RGATCY 4 cut(s) 394, 431, 1256, 1697
MhlI GDGCHC 3 cut(s) 182, 633, 1066
MluCI AATT 8 cut(s) 196, 386, 477, 534, 746, 780, 1336, 1538
MlyI GAGTC 3 cut(s) 508, 1097, 1292
MmeI TCCRAC 1 cut(s) 1071
Mph1103I ATGCAT 2 cut(s) 581, 869
MroXI GAANNNNTTC 2 cut(s) 1098, 1131
MseI TTAA 4 cut(s) 582, 1325, 1542, 1592
MslI CAYNNNNRTG 2 cut(s) 1266, 1650
MspI CCGG 3 cut(s) 138, 152, 1608
MspR9I CCNGG 3 cut(s) 139, 737, 823
MunI CAATTG 2 cut(s) 196, 746
Mva1269I GAATGC 2 cut(s) 235, 676
MvaI CCWGG 2 cut(s) 737, 823
MvnI CGCG 3 cut(s) 730, 732, 1155
MwoI GCNNNNNNNGC 3 cut(s) 217, 860, 869
NciI CCSGG 1 cut(s) 139
NdeI CATATG 2 cut(s) 556, 1586
NheI GCTAGC 1 cut(s) 1138
NlaIII CATG 6 cut(s) 211, 271, 796, 867, 1453, 1649
NlaIV GGNNCC 2 cut(s) 156, 1064
NmuCI GTSAC 1 cut(s) 524
NsiI ATGCAT 2 cut(s) 581, 869
NspI RCATGY 2 cut(s) 867, 1649
NspV TTCGAA 1 cut(s) 1514
PaeI GCATGC 1 cut(s) 867
PagI TCATGA 1 cut(s) 792
PauI GCGCGC 1 cut(s) 728
PceI AGGCCT 1 cut(s) 1319
PciSI GCTCTTC 1 cut(s) 660
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PctI GAATGC 2 cut(s) 235, 676
PdmI GAANNNNTTC 2 cut(s) 1098, 1131
PfeI GAWTC 6 cut(s) 341, 551, 1302, 1415, 1511, 1733
PleI GAGTC 3 cut(s) 508, 1096, 1291
PpsI GAGTC 3 cut(s) 508, 1096, 1291
Ppu21I YACGTR 1 cut(s) 851
Psp124BI GAGCTC 1 cut(s) 633
Psp6I CCWGG 2 cut(s) 735, 821
PspGI CCWGG 2 cut(s) 735, 821
PspN4I GGNNCC 2 cut(s) 156, 1064
PspOMI GGGCCC 1 cut(s) 1062
PspPI GGNCC 3 cut(s) 114, 1062, 1063
PsuI RGATCY 4 cut(s) 394, 431, 1256, 1697
PteI GCGCGC 1 cut(s) 728
RsaI GTAC 4 cut(s) 101, 148, 788, 1193
RsaNI GTAC 4 cut(s) 100, 147, 787, 1192
RseI CAYNNNNRTG 2 cut(s) 1266, 1650
SacI GAGCTC 1 cut(s) 633
SapI GCTCTTC 1 cut(s) 660
SaqAI TTAA 4 cut(s) 582, 1325, 1542, 1592
Sau96I GGNCC 3 cut(s) 114, 1062, 1063
SchI GAGTC 3 cut(s) 508, 1097, 1292
ScrFI CCNGG 3 cut(s) 139, 737, 823
SduI GDGCHC 3 cut(s) 182, 633, 1066
SfaNI GCATC 2 cut(s) 1489, 1666
SfcI CTRYAG 1 cut(s) 858
SfuI TTCGAA 1 cut(s) 1514
SgrAI CRCCGGYG 1 cut(s) 151
SmiMI CAYNNNNRTG 2 cut(s) 1266, 1650
SmlI CTYRAG 4 cut(s) 986, 1252, 1313, 1638
SmoI CTYRAG 4 cut(s) 986, 1252, 1313, 1638
SphI GCATGC 1 cut(s) 867
Sse9I AATT 8 cut(s) 196, 386, 477, 534, 746, 780, 1336, 1538
SseBI AGGCCT 1 cut(s) 1319
SsiI CCGC 7 cut(s) 16, 112, 191, 294, 380, 464, 1214
SspMI CTAG 2 cut(s) 1139, 1241
SstI GAGCTC 1 cut(s) 633
StuI AGGCCT 1 cut(s) 1319
StyD4I CCNGG 3 cut(s) 137, 735, 821
StyI CCWWGG 3 cut(s) 87, 240, 624
TaaI ACNGT 2 cut(s) 530, 918
TaiI ACGT 2 cut(s) 302, 853
TaqI TCGA 4 cut(s) 30, 143, 571, 1514
TasI AATT 8 cut(s) 196, 386, 477, 534, 746, 780, 1336, 1538
TauI GCSGC 4 cut(s) 193, 296, 382, 1217
TfiI GAWTC 6 cut(s) 341, 551, 1302, 1415, 1511, 1733
Tru1I TTAA 4 cut(s) 582, 1325, 1542, 1592
Tru9I TTAA 4 cut(s) 582, 1325, 1542, 1592
TscAI CASTG 2 cut(s) 535, 1282
TseFI GTSAC 1 cut(s) 524
TseI GCWGC 6 cut(s) 42, 232, 670, 673, 818, 854
Tsp45I GTSAC 1 cut(s) 524
TspDTI ATGAA 8 cut(s) 330, 564, 731, 809, 1187, 1340, 1421, 1422
TspGWI ACGGA 2 cut(s) 926, 1710
TspRI CASTG 2 cut(s) 535, 1282
XapI RAATTY 3 cut(s) 386, 477, 1336
XceI RCATGY 2 cut(s) 867, 1649
XmnI GAANNNNTTC 2 cut(s) 1098, 1131
XspI CTAG 2 cut(s) 1139, 1241
Zsp2I ATGCAT 2 cut(s) 581, 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.