Rw5G029200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014697
Physical Location & Seq
Forward (+)
44579050 .. 44581830
2781 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G029200.1

Sequence Viewer

Length: 1671 bp
ATGTCTTTGCAGAACCGCCACGACTTGATCGAGAGGCAATGCAGCAAGATCAGGAAGCGAGGCTGTTCGTCTTCGTCCTCGTCTTCCTTGGTGCGTAGGTACAGGTTGAAGCGGGCCATTCTTGTCGGGAAGCGAGCCGGGTCGAGTACGCCGGTGCCAACTTGGAAGACTTCAACGAGCCCCAGATCGCCGCCCAATTGCGCCACCATGCCGAAGAAGCAGAAGGGTCAGCAGCATTCCAAGGACGACGGGAAAGGGAAGGAAGTCATGTCAGTCTCAGCAAGAAAGCTCGCCGCCACGTTGTGGGAGATCAATGAAGTCCCTGATGTGAAAAGCAAAGATTCTAAAATCAAAGAGGAAGAGAGAAAAGCTGGATCTGGAGGTGCACACGAGAGAAGATTGTCGGTTTCTCAGAGAATTCAACCGACTGATCACTACTTGGGAGGGTTTGAGACTCATAGCAGTCACAGTGTAATTGAGGTCAAAGATGAATCATATGGAAAAACTGATCGAAAATGCATTAAGACCTGTTTGAAGGAGGTCAGCAATGGCCTTTCTACATCCAAGGAGCTCGTAAAAGTTCTAACTCGTGTTTCTGGTCTTGAAGAGCAGCAGCATTCTCTAAGTATGCCCCTGCTCTCAGCCTTACGAGTTGAACTTGATCGAGCGCGCGTCCAGGTTCATCAATTGATCCGAGAACAGAGGTCAAACTGCAAAGAAATTGAGTACCTCATGAAGCATTTTGCAGAAGAAAAGGCAGCCTGGAAAAGAAAGGAGCGAGAGAAATACAAGAAGCTGAGAAAACAAACAGAACGGTTGAACAAAAAGCTTGGCAAAGAACTGGAAGATAAAAATGTAGCTCTGTCAAAGGCAACAAAAGAACTTGAGAGGGAGAAGAGAGCAAAAGAGATTTTTGAGCAAGTTTGTGATGAGTTAGCCGTAGGTATGGGGGAAGACAGGGCCCAAGTTGAAGAACTAAAGAGAGAGTCAGAAAAAGTTCGTGAAGAGGTGGAAAAGGAGAGGGAAATGCTTCAGCTAGCAGATGTGTTGCGCGAGGAGAGAGTTCAGATGAAGCTCTCGGAAGCAAAGTACCATTTTGAAGAAAAAAATGCGGCTGTTGAGCAACTGAAGAATGAGCTAGAGGGCTACTTGAGATCCCATAAGGGTGAAGGCAGTGGTGAGTCTCCAGACTTCAAAAGAATCAAGGAACTTGAGGCCTACTTAAAGAAAATAAATTTTGGGTCATTTCAGAGCATAAAGGAAGGAGACGATGGAATGGAAGTTGCATACAGAGAAGAGTGTGATGAAGAAGATTCAGGTGATGATCTTCATTCCATTGAACTAAACATGGACAATGGTAATAGGAGCTACAAGTGGAGTTATGCTTGTGGAGATGATGCTGAAGATGATTCAAAAAGGAGTTCAGTTGATAAACAATTTAAAGGAAGAAGGTCTCTCTCTGAAAAGATACAATGGGAAAACATATGCTTAAACAAAAGTTCCACCGGCATTGATTGGGGATTTGGCTCAAAAACTCAAGAACATGCAGATGGGCATCAAACACAAGAACACGAAACTGAAATCAAGAAAAATGGATCTACTGTATTTCAATGCTTTGCCACTACAACTTGCCAGTACCAATGCTTGCCTATGAGGGATCCGCGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

556

Amino Acids

63.82

Weight (kDa)

8.64

Isoelectric Point (pI)

51.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014697)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g25790 FvH4_3g25790
malus_domestica MD03G1150600.v1.1 MD11G1170000.v1.1
prunus_persica Prupe.6G137500_v2.0.a1
pyrus_communis pycom03g10470 pycom11g14240
rosa_chinensis RchiOBHm_Chr5g0047011
rosa_laevigata RLG00000034458
rosa_multiflora Rmu_sc0005544.1_g000006
rosa_roxburghii Rroxscaffold_1G00034420
rosa_rugosa Rorug05G0233200
rosa_samantha Rh5AG312400 Rh5BG321500 Rh5CG347700 Rh5DG332400
rosa_wichuraiana Rw5G029200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 154
AccB7I CCANNNNNTGG 1 cut(s) 303
AccII CGCG 4 cut(s) 670, 672, 1053, 1663
AciI CCGC 6 cut(s) 16, 112, 191, 294, 1112, 1661
AclWI GGATC 6 cut(s) 382, 685, 1149, 1603, 1652, 1665
AcsI RAATTY 2 cut(s) 417, 1234
AcuI CTGAAG 3 cut(s) 1016, 1148, 1422
AdeI CACNNNGTG 1 cut(s) 303
AfaI GTAC 5 cut(s) 101, 148, 728, 1091, 1637
AfiI CCNNNNNNNGG 1 cut(s) 303
AjnI CCWGG 2 cut(s) 675, 761
Alw21I GWGCWC 2 cut(s) 388, 573
Alw26I GTCTC 5 cut(s) 280, 446, 1188, 1260, 1458
Alw44I GTGCAC 1 cut(s) 384
AlwI GGATC 6 cut(s) 382, 685, 1149, 1603, 1652, 1665
AoxI GGCC 4 cut(s) 114, 550, 960, 1215
ApaI GGGCCC 1 cut(s) 964
ApaLI GTGCAC 1 cut(s) 384
ApeKI GCWGC 5 cut(s) 42, 232, 610, 613, 758
ApoI RAATTY 2 cut(s) 417, 1234
Asp700I GAANNNNTTC 2 cut(s) 996, 1029
AspLEI GCGC 4 cut(s) 203, 670, 672, 1053
AspS9I GGNCC 3 cut(s) 114, 960, 961
AsuC2I CCSGG 1 cut(s) 139
AsuHPI GGTGA 3 cut(s) 1178, 1190, 1331
AsuNHI GCTAGC 1 cut(s) 1036
BaeGI GKGCMC 2 cut(s) 388, 964
BamHI GGATCC 1 cut(s) 1657
BanI GGYRCC 1 cut(s) 154
BanII GRGCYC 3 cut(s) 182, 573, 964
BauI CACGAG 2 cut(s) 389, 588
BbsI GAAGAC 4 cut(s) 63, 75, 173, 960
Bbv12I GWGCWC 2 cut(s) 388, 573
BbvI GCAGC 5 cut(s) 54, 244, 622, 625, 770
BccI CCATC 2 cut(s) 1265, 1544
BceAI ACGGC 1 cut(s) 923
BciT130I CCWGG 2 cut(s) 677, 763
BclI TGATCA 1 cut(s) 430
BcnI CCSGG 1 cut(s) 139
BcoDI GTCTC 5 cut(s) 280, 446, 1188, 1260, 1458
BfaI CTAG 2 cut(s) 1037, 1139
BisI GCNGC 8 cut(s) 43, 191, 233, 294, 611, 614, 759, 1113
BlsI GCNGC 8 cut(s) 44, 192, 234, 295, 612, 615, 760, 1114
Bme1390I CCNGG 3 cut(s) 139, 677, 763
BmgT120I GGNCC 3 cut(s) 114, 960, 961
BmiI GGNNCC 3 cut(s) 156, 962, 1659
BmrFI CCNGG 3 cut(s) 139, 677, 763
BmsI GCATC 2 cut(s) 1387, 1564
BmtI GCTAGC 1 cut(s) 1040
BpiI GAAGAC 4 cut(s) 63, 75, 173, 960
BpmI CTGGAG 2 cut(s) 399, 1170
BpuEI CTTGAG 4 cut(s) 905, 1171, 1232, 1521
BpuMI CCSGG 1 cut(s) 139
BsaBI GATNNNNATC 1 cut(s) 1554
BsaI GGTCTC 1 cut(s) 1458
BsaJI CCNNGG 3 cut(s) 87, 240, 564
Bsc4I CCNNNNNNNGG 1 cut(s) 303
Bse118I RCCGGY 2 cut(s) 151, 1505
Bse1I ACTGG 2 cut(s) 846, 1633
Bse3DI GCAATG 2 cut(s) 44, 553
Bse8I GATNNNNATC 1 cut(s) 1554
BseBI CCWGG 2 cut(s) 677, 763
BseDI CCNNGG 3 cut(s) 87, 240, 564
BseGI GGATG 1 cut(s) 560
BseJI GATNNNNATC 1 cut(s) 1554
BseLI CCNNNNNNNGG 1 cut(s) 303
BseMI GCAATG 2 cut(s) 44, 553
BseMII CTCAG 4 cut(s) 291, 425, 654, 788
BseNI ACTGG 2 cut(s) 846, 1633
BsePI GCGCGC 1 cut(s) 668
BseRI GAGGAG 1 cut(s) 1070
BseSI GKGCMC 2 cut(s) 388, 964
BseXI GCAGC 5 cut(s) 54, 244, 622, 625, 770
Bsh1236I CGCG 4 cut(s) 670, 672, 1053, 1663
BshFI GGCC 4 cut(s) 116, 552, 962, 1217
BshNI GGYRCC 1 cut(s) 154
BsiHKAI GWGCWC 2 cut(s) 388, 573
BsiSI CCGG 3 cut(s) 138, 152, 1506
BslFI GGGAC 1 cut(s) 305
BslI CCNNNNNNNGG 1 cut(s) 303
BsmAI GTCTC 5 cut(s) 280, 446, 1188, 1260, 1458
BsmBI CGTCTC 1 cut(s) 1260
BsmFI GGGAC 1 cut(s) 305
BsmI GAATGC 2 cut(s) 235, 616
BsnI GGCC 4 cut(s) 116, 552, 962, 1217
Bso31I GGTCTC 1 cut(s) 1458
Bsp120I GGGCCC 1 cut(s) 960
Bsp1286I GDGCHC 4 cut(s) 182, 388, 573, 964
BspACI CCGC 6 cut(s) 16, 112, 191, 294, 1112, 1661
BspANI GGCC 4 cut(s) 116, 552, 962, 1217
BspCNI CTCAG 4 cut(s) 290, 424, 653, 789
BspFNI CGCG 4 cut(s) 670, 672, 1053, 1663
BspHI TCATGA 1 cut(s) 732
BspLI GGNNCC 3 cut(s) 156, 962, 1659
BspOI GCTAGC 1 cut(s) 1040
BspPI GGATC 6 cut(s) 382, 685, 1149, 1603, 1652, 1665
BspQI GCTCTTC 1 cut(s) 600
BspT107I GGYRCC 1 cut(s) 154
BspTNI GGTCTC 1 cut(s) 1458
BsrDI GCAATG 2 cut(s) 44, 553
BsrFI RCCGGY 2 cut(s) 151, 1505
BsrI ACTGG 2 cut(s) 846, 1633
BssAI RCCGGY 2 cut(s) 151, 1505
BssECI CCNNGG 3 cut(s) 87, 240, 564
BssHII GCGCGC 1 cut(s) 668
BssSI CACGAG 2 cut(s) 389, 588
BssT1I CCWWGG 3 cut(s) 87, 240, 564
Bst2BI CACGAG 2 cut(s) 389, 588
Bst2UI CCWGG 2 cut(s) 677, 763
Bst4CI ACNGT 3 cut(s) 470, 816, 1603
Bst6I CTCTTC 5 cut(s) 354, 600, 890, 999, 1290
BstC8I GCNNGC 6 cut(s) 114, 135, 291, 670, 1038, 1646
BstDEI CTNAG 5 cut(s) 277, 411, 623, 640, 797
BstF5I GGATG 1 cut(s) 560
BstFNI CGCG 4 cut(s) 670, 672, 1053, 1663
BstHHI GCGC 4 cut(s) 203, 670, 672, 1053
BstMAI GTCTC 5 cut(s) 280, 446, 1188, 1260, 1458
BstMWI GCNNNNNNNGC 1 cut(s) 217
BstNI CCWGG 2 cut(s) 677, 763
BstNSI RCATGY 1 cut(s) 1547
BstSCI CCNGG 3 cut(s) 137, 675, 761
BstSLI GKGCMC 2 cut(s) 388, 964
BstUI CGCG 4 cut(s) 670, 672, 1053, 1663
BstV1I GCAGC 5 cut(s) 54, 244, 622, 625, 770
BstV2I GAAGAC 4 cut(s) 63, 75, 173, 960
BstX2I RGATCY 4 cut(s) 374, 1154, 1595, 1657
BstYI RGATCY 4 cut(s) 374, 1154, 1595, 1657
BsuRI GGCC 4 cut(s) 116, 552, 962, 1217
BtsCI GGATG 1 cut(s) 560
BtsI GCAGTG 1 cut(s) 1180
BtsIMutI CAGTG 2 cut(s) 475, 1180
Cac8I GCNNGC 6 cut(s) 114, 135, 291, 670, 1038, 1646
CciI TCATGA 1 cut(s) 732
CfoI GCGC 4 cut(s) 203, 670, 672, 1053
Cfr10I RCCGGY 2 cut(s) 151, 1505
Cfr13I GGNCC 3 cut(s) 114, 960, 961
CseI GACGC 1 cut(s) 661
Csp6I GTAC 5 cut(s) 100, 147, 727, 1090, 1636
CspCI CAANNNNNGTGG 2 cut(s) 1492, 1527
CviAII CATG 5 cut(s) 208, 268, 733, 1348, 1544
CviQI GTAC 5 cut(s) 100, 147, 727, 1090, 1636
DdeI CTNAG 5 cut(s) 277, 411, 623, 640, 797
DraI TTTAAA 1 cut(s) 1441
DraIII CACNNNGTG 1 cut(s) 303
Eam1104I CTCTTC 5 cut(s) 354, 600, 890, 999, 1290
EarI CTCTTC 5 cut(s) 354, 600, 890, 999, 1290
Ecl136II GAGCTC 1 cut(s) 571
Eco130I CCWWGG 3 cut(s) 87, 240, 564
Eco147I AGGCCT 1 cut(s) 1217
Eco24I GRGCYC 3 cut(s) 182, 573, 964
Eco31I GGTCTC 1 cut(s) 1458
Eco53kI GAGCTC 1 cut(s) 571
Eco57I CTGAAG 3 cut(s) 1016, 1148, 1422
EcoICRI GAGCTC 1 cut(s) 571
EcoO109I RGGNCCY 1 cut(s) 960
EcoRI GAATTC 1 cut(s) 417
EcoRII CCWGG 2 cut(s) 675, 761
EcoT14I CCWWGG 3 cut(s) 87, 240, 564
EcoT22I ATGCAT 1 cut(s) 521
EcoT38I GRGCYC 3 cut(s) 182, 573, 964
ErhI CCWWGG 3 cut(s) 87, 240, 564
Esp3I CGTCTC 1 cut(s) 1260
FaeI CATG 5 cut(s) 211, 271, 736, 1351, 1547
FaqI GGGAC 1 cut(s) 305
FatI CATG 5 cut(s) 207, 267, 732, 1347, 1543
FauI CCCGC 1 cut(s) 105
FauNDI CATATG 2 cut(s) 496, 1484
FbaI TGATCA 1 cut(s) 430
Fnu4HI GCNGC 8 cut(s) 43, 191, 233, 294, 611, 614, 759, 1113
FokI GGATG 1 cut(s) 547
FriOI GRGCYC 3 cut(s) 182, 573, 964
Fsp4HI GCNGC 8 cut(s) 43, 191, 233, 294, 611, 614, 759, 1113
FspBI CTAG 2 cut(s) 1037, 1139
GlaI GCGC 4 cut(s) 202, 669, 671, 1052
GluI GCNGC 8 cut(s) 43, 191, 233, 294, 611, 614, 759, 1113
GsuI CTGGAG 2 cut(s) 399, 1170
HaeIII GGCC 4 cut(s) 116, 552, 962, 1217
HapII CCGG 3 cut(s) 138, 152, 1506
HgaI GACGC 1 cut(s) 661
HhaI GCGC 4 cut(s) 203, 670, 672, 1053
Hin1II CATG 5 cut(s) 211, 271, 736, 1351, 1547
Hin6I GCGC 4 cut(s) 201, 668, 670, 1051
HinP1I GCGC 4 cut(s) 201, 668, 670, 1051
HindIII AAGCTT 1 cut(s) 827
HinfI GANTC 8 cut(s) 341, 454, 491, 986, 1181, 1200, 1313, 1409
HpaII CCGG 3 cut(s) 138, 152, 1506
HphI GGTGA 3 cut(s) 1178, 1190, 1331
Hpy166II GTNNAC 1 cut(s) 386
Hpy188I TCNGA 7 cut(s) 414, 695, 991, 1068, 1081, 1251, 1462
Hpy8I GTNNAC 1 cut(s) 386
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 6 cut(s) 217, 253, 529, 1163, 1256, 1443
HpyCH4III ACNGT 3 cut(s) 470, 816, 1603
HpyCH4IV ACGT 1 cut(s) 299
HpyCH4V TGCA 8 cut(s) 10, 42, 386, 519, 714, 746, 1286, 1547
HpyF10VI GCNNNNNNNGC 1 cut(s) 217
HpyF3I CTNAG 5 cut(s) 277, 411, 623, 640, 797
HpySE526I ACGT 1 cut(s) 299
Hsp92II CATG 5 cut(s) 211, 271, 736, 1351, 1547
HspAI GCGC 4 cut(s) 201, 668, 670, 1051
Ksp22I TGATCA 1 cut(s) 430
LguI GCTCTTC 1 cut(s) 600
LmnI GCTCC 3 cut(s) 568, 775, 1365
Lsp1109I GCAGC 5 cut(s) 54, 244, 622, 625, 770
LweI GCATC 2 cut(s) 1387, 1564
MaeI CTAG 2 cut(s) 1037, 1139
MaeII ACGT 1 cut(s) 299
MaeIII GTNAC 1 cut(s) 464
MfeI CAATTG 2 cut(s) 196, 686
MflI RGATCY 4 cut(s) 374, 1154, 1595, 1657
MhlI GDGCHC 4 cut(s) 182, 388, 573, 964
MluCI AATT 7 cut(s) 196, 417, 474, 686, 720, 1234, 1436
MlyI GAGTC 3 cut(s) 448, 995, 1190
Mph1103I ATGCAT 1 cut(s) 521
MroXI GAANNNNTTC 2 cut(s) 996, 1029
MseI TTAA 4 cut(s) 522, 1223, 1440, 1490
MslI CAYNNNNRTG 2 cut(s) 1164, 1548
MspI CCGG 3 cut(s) 138, 152, 1506
MspR9I CCNGG 3 cut(s) 139, 677, 763
MunI CAATTG 2 cut(s) 196, 686
Mva1269I GAATGC 2 cut(s) 235, 616
MvaI CCWGG 2 cut(s) 677, 763
MvnI CGCG 4 cut(s) 670, 672, 1053, 1663
MwoI GCNNNNNNNGC 1 cut(s) 217
NciI CCSGG 1 cut(s) 139
NdeI CATATG 2 cut(s) 496, 1484
NheI GCTAGC 1 cut(s) 1036
NlaIII CATG 5 cut(s) 211, 271, 736, 1351, 1547
NlaIV GGNNCC 3 cut(s) 156, 962, 1659
NmuCI GTSAC 1 cut(s) 464
NsiI ATGCAT 1 cut(s) 521
NspI RCATGY 1 cut(s) 1547
PagI TCATGA 1 cut(s) 732
PauI GCGCGC 1 cut(s) 668
PceI AGGCCT 1 cut(s) 1217
PciSI GCTCTTC 1 cut(s) 600
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PctI GAATGC 2 cut(s) 235, 616
PdmI GAANNNNTTC 2 cut(s) 996, 1029
PfeI GAWTC 5 cut(s) 341, 491, 1200, 1313, 1409
PflMI CCANNNNNTGG 1 cut(s) 303
PkrI GCNGC 8 cut(s) 44, 192, 234, 295, 612, 615, 760, 1114
PleI GAGTC 3 cut(s) 448, 994, 1189
PpsI GAGTC 3 cut(s) 448, 994, 1189
Psp124BI GAGCTC 1 cut(s) 573
Psp6I CCWGG 2 cut(s) 675, 761
PspGI CCWGG 2 cut(s) 675, 761
PspN4I GGNNCC 3 cut(s) 156, 962, 1659
PspOMI GGGCCC 1 cut(s) 960
PspPI GGNCC 3 cut(s) 114, 960, 961
PsuI RGATCY 4 cut(s) 374, 1154, 1595, 1657
PteI GCGCGC 1 cut(s) 668
RsaI GTAC 5 cut(s) 101, 148, 728, 1091, 1637
RsaNI GTAC 5 cut(s) 100, 147, 727, 1090, 1636
RseI CAYNNNNRTG 2 cut(s) 1164, 1548
SacI GAGCTC 1 cut(s) 573
SapI GCTCTTC 1 cut(s) 600
SaqAI TTAA 4 cut(s) 522, 1223, 1440, 1490
SatI GCNGC 8 cut(s) 43, 191, 233, 294, 611, 614, 759, 1113
Sau96I GGNCC 3 cut(s) 114, 960, 961
SchI GAGTC 3 cut(s) 448, 995, 1190
ScrFI CCNGG 3 cut(s) 139, 677, 763
SduI GDGCHC 4 cut(s) 182, 388, 573, 964
SfaNI GCATC 2 cut(s) 1387, 1564
SgrAI CRCCGGYG 1 cut(s) 151
SmiMI CAYNNNNRTG 2 cut(s) 1164, 1548
SmlI CTYRAG 4 cut(s) 884, 1150, 1211, 1536
SmoI CTYRAG 4 cut(s) 884, 1150, 1211, 1536
Sse9I AATT 7 cut(s) 196, 417, 474, 686, 720, 1234, 1436
SseBI AGGCCT 1 cut(s) 1217
SsiI CCGC 6 cut(s) 16, 112, 191, 294, 1112, 1661
SspMI CTAG 2 cut(s) 1037, 1139
SstI GAGCTC 1 cut(s) 573
StuI AGGCCT 1 cut(s) 1217
StyD4I CCNGG 3 cut(s) 137, 675, 761
StyI CCWWGG 3 cut(s) 87, 240, 564
TaaI ACNGT 3 cut(s) 470, 816, 1603
TaiI ACGT 1 cut(s) 302
TaqI TCGA 4 cut(s) 30, 143, 511, 664
TasI AATT 7 cut(s) 196, 417, 474, 686, 720, 1234, 1436
TauI GCSGC 3 cut(s) 193, 296, 1115
TfiI GAWTC 5 cut(s) 341, 491, 1200, 1313, 1409
Tru1I TTAA 4 cut(s) 522, 1223, 1440, 1490
Tru9I TTAA 4 cut(s) 522, 1223, 1440, 1490
TscAI CASTG 2 cut(s) 475, 1180
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 5 cut(s) 42, 232, 610, 613, 758
Tsp45I GTSAC 1 cut(s) 464
TspDTI ATGAA 7 cut(s) 330, 504, 671, 749, 1085, 1319, 1320
TspRI CASTG 2 cut(s) 475, 1180
Van91I CCANNNNNTGG 1 cut(s) 303
VneI GTGCAC 1 cut(s) 384
XapI RAATTY 2 cut(s) 417, 1234
XceI RCATGY 1 cut(s) 1547
XmnI GAANNNNTTC 2 cut(s) 996, 1029
XspI CTAG 2 cut(s) 1037, 1139
Zsp2I ATGCAT 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.