FvH4_3g26270

Outer envelope protein 80

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0015354
Physical Location & Seq
Reverse (-)
19209843 .. 19215821
5979 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g26270.t1

Sequence Viewer

Length: 1086 bp
ATGGGAGCTCAGAAGAGCATCCATGCTGGCAAAGCCAAGATTGATGTTAACGTTGATTTCACTCACAAGCTCTGTGCCTCTTTGATGCTTCCTCCATTGAGTAATGGCAGTGGCAGTCCTCTCTCTCTTATCATTGGGAGTCTCTGCATTAAACATCCAAACTTGTTCGGTGGAAGTGAGAAACTTGATGTGTCATTGGATAAGGGCCTATATGATTCAAATGTCTTATTGGCCTATAGAAGGCCAAGACCTGAATGGCTAGCTCAACAGTCATTTGTACTACAGCATTCTATTTCACCCGAGATAGGGGTCCATGGCATTCCCATGGACAATTTTTCCCGGTCAGGTAGTGGAGGTGTAAATTTGTCTCGACTATCAGTAGGCATGGATCTCAACGAGCCTGCAAGTTCCAAGTGGAGTAGCACAACTAGCATAAAGTTTGAGCATGTCCGGCCACTGAATGATAATGGCCGCTCAATGAGCCGAGACCTTGATGGGTTTCCTGTCACATGCAGTGGAAGTCCTCATGATAGTATGGTAGTTCTAAAGCAAGAATCTCGGTATGCAAAGGCAAATGATCACAGGTTTTTTCATTTCAGTTTGCAAATAGAACAAGGAATACCAGTTCTATCAAAGTGGCTAATATTTAACAAGTTTAAATTTATTGCATCAAAGGGAGTCAAACTTGGCCCAGCATTCCTCTTGACAAGAATGACAGGTGGTTCAATTGTGGGGGATATGGCTCCTTACCAAGCATTTGCAATTGGCGGTCTTGGCAGTGTGCGAGGGTATGGTGAGGGTGCAGTAGGATCAGGAAGGACATGCCTTGTTGCAAATACCGAATTAACATTACCTATTAACAAGATGCTGGAAGGTGCTGTTTTTTTGGATTGTGGAACTGATTTGGGATCTGCTCATCATGTACCAGGAAACCCAGCCCTGAGGCTAGGGAAACCAGGATCTGGAATAGGGCTTGGATATGGTCTTCGTTTGAAATCACAGTTTGGTCATTTTGTGGTTGATTACGCTATTAATGCCTTGCACCAAAAAACACTCTATTTTGGCATCAGCAACCTTGCTTCATGA

Protein Analysis

362

Amino Acids

38.7

Weight (kDa)

9.3

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Omp85 PF01103 54 - 357 1.6e-31 Omp85 superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44160
fragaria_vesca FvH4_3g26270
malus_domestica MD11G1174200.v1.1
prunus_persica Prupe.6G146000_v2.0.a1
pyrus_communis pycom11g14900
rosa_chinensis RchiOBHm_Chr5g0048701
rosa_laevigata RLG00000034550
rosa_multiflora Rmu_sc0006432.1_g000004
rosa_roxburghii Rroxscaffold_1G00033100
rosa_rugosa Rorug05G0240700
rosa_samantha Rh5AG320500 Rh5BG330200 Rh5CG356200 Rh5DG341400
rosa_wichuraiana Rw5G030170 Rw5G030260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 962
AccBSI CCGCTC 1 cut(s) 474
AciI CCGC 2 cut(s) 472, 768
AclI AACGTT 1 cut(s) 51
AclWI GGATC 4 cut(s) 396, 817, 916, 967
AcoI YGGCCR 2 cut(s) 452, 469
AcsI RAATTY 2 cut(s) 361, 659
AfaI GTAC 2 cut(s) 279, 924
AfiI CCNNNNNNNGG 4 cut(s) 240, 305, 306, 962
AgsI TTSAA 3 cut(s) 219, 726, 994
AjnI CCWGG 2 cut(s) 925, 955
AloI GAACNNNNNNTCC 2 cut(s) 609, 641
AluBI AGCT 3 cut(s) 8, 70, 263
AluI AGCT 3 cut(s) 8, 70, 263
Alw21I GWGCWC 1 cut(s) 10
Alw26I GTCTC 3 cut(s) 146, 372, 480
AlwI GGATC 4 cut(s) 396, 817, 916, 967
AlwNI CAGNNNCTG 1 cut(s) 962
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 6 cut(s) 205, 231, 242, 452, 469, 688
ApoI RAATTY 2 cut(s) 361, 659
AseI ATTAAT 1 cut(s) 1032
AspS9I GGNCC 3 cut(s) 205, 310, 689
AsuC2I CCSGG 1 cut(s) 340
AsuHPI GGTGA 2 cut(s) 288, 806
AsuNHI GCTAGC 1 cut(s) 259
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 1 cut(s) 310
AxyI CCTNAGG 1 cut(s) 941
BanII GRGCYC 1 cut(s) 10
BbsI GAAGAC 1 cut(s) 977
Bbv12I GWGCWC 1 cut(s) 10
BccI CCATC 1 cut(s) 488
BcgI CGANNNNNNTGC 2 cut(s) 539, 573
BciT130I CCWGG 2 cut(s) 927, 957
BclI TGATCA 1 cut(s) 577
BcnI CCSGG 1 cut(s) 340
BcoDI GTCTC 3 cut(s) 146, 372, 480
BfaI CTAG 3 cut(s) 260, 429, 947
BfmI CTRYAG 2 cut(s) 235, 281
BisI GCNGC 1 cut(s) 472
BlsI GCNGC 1 cut(s) 473
Bme1390I CCNGG 3 cut(s) 340, 927, 957
Bme18I GGWCC 1 cut(s) 310
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 3 cut(s) 205, 310, 689
BmiI GGNNCC 2 cut(s) 311, 744
BmrFI CCNGG 3 cut(s) 340, 927, 957
BmsI GCATC 5 cut(s) 27, 75, 677, 855, 1074
BmtI GCTAGC 1 cut(s) 263
BpiI GAAGAC 1 cut(s) 977
BpuMI CCSGG 1 cut(s) 340
BsaI GGTCTC 1 cut(s) 480
BsaJI CCNNGG 2 cut(s) 313, 324
Bsc4I CCNNNNNNNGG 4 cut(s) 240, 305, 306, 962
Bse1I ACTGG 1 cut(s) 623
Bse21I CCTNAGG 1 cut(s) 941
BseBI CCWGG 2 cut(s) 927, 957
BseDI CCNNGG 2 cut(s) 313, 324
BseGI GGATG 2 cut(s) 18, 154
BseLI CCNNNNNNNGG 4 cut(s) 240, 305, 306, 962
BseMII CTCAG 2 cut(s) 23, 932
BseNI ACTGG 1 cut(s) 623
BseYI CCCAGC 2 cut(s) 691, 934
BsgI GTGCAG 1 cut(s) 822
BshFI GGCC 6 cut(s) 207, 233, 244, 454, 471, 690
BsiHKAI GWGCWC 1 cut(s) 10
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 2 cut(s) 340, 451
BslI CCNNNNNNNGG 4 cut(s) 240, 305, 306, 962
BsmAI GTCTC 3 cut(s) 146, 372, 480
BsmI GAATGC 3 cut(s) 286, 318, 695
BsnI GGCC 6 cut(s) 207, 233, 244, 454, 471, 690
Bso31I GGTCTC 1 cut(s) 480
BsoBI CYCGRG 1 cut(s) 299
Bsp1286I GDGCHC 1 cut(s) 10
Bsp143I GATC 5 cut(s) 388, 577, 809, 908, 959
Bsp19I CCATGG 2 cut(s) 313, 324
BspACI CCGC 2 cut(s) 472, 768
BspANI GGCC 6 cut(s) 207, 233, 244, 454, 471, 690
BspCNI CTCAG 2 cut(s) 22, 933
BspHI TCATGA 2 cut(s) 526, 1082
BspLI GGNNCC 2 cut(s) 311, 744
BspOI GCTAGC 1 cut(s) 263
BspPI GGATC 4 cut(s) 396, 817, 916, 967
BspQI GCTCTTC 1 cut(s) 8
BspTNI GGTCTC 1 cut(s) 480
BsrBI CCGCTC 1 cut(s) 474
BsrI ACTGG 1 cut(s) 623
BssECI CCNNGG 2 cut(s) 313, 324
BssMI GATC 5 cut(s) 388, 577, 809, 908, 959
BssT1I CCWWGG 2 cut(s) 313, 324
Bst2UI CCWGG 2 cut(s) 927, 957
Bst4CI ACNGT 2 cut(s) 270, 1002
Bst6I CTCTTC 1 cut(s) 8
BstC8I GCNNGC 3 cut(s) 28, 261, 402
BstDEI CTNAG 2 cut(s) 9, 941
BstDSI CCRYGG 2 cut(s) 313, 324
BstENI CCTNNNNNAGG 1 cut(s) 238
BstF5I GGATG 2 cut(s) 18, 154
BstKTI GATC 5 cut(s) 391, 580, 812, 911, 962
BstMAI GTCTC 3 cut(s) 146, 372, 480
BstMBI GATC 5 cut(s) 388, 577, 809, 908, 959
BstMWI GCNNNNNNNGC 6 cut(s) 32, 429, 451, 480, 774, 1034
BstNI CCWGG 2 cut(s) 927, 957
BstNSI RCATGY 3 cut(s) 449, 513, 825
BstSCI CCNGG 3 cut(s) 338, 925, 955
BstSFI CTRYAG 2 cut(s) 235, 281
BstV2I GAAGAC 1 cut(s) 977
BstX2I RGATCY 3 cut(s) 388, 908, 959
BstYI RGATCY 3 cut(s) 388, 908, 959
Bsu36I CCTNAGG 1 cut(s) 941
BsuRI GGCC 6 cut(s) 207, 233, 244, 454, 471, 690
BtgI CCRYGG 2 cut(s) 313, 324
BtsCI GGATG 2 cut(s) 18, 154
BtsI GCAGTG 3 cut(s) 115, 520, 784
BtsIMutI CAGTG 4 cut(s) 115, 455, 520, 784
Cac8I GCNNGC 3 cut(s) 28, 261, 402
CaiI CAGNNNCTG 1 cut(s) 962
CciI TCATGA 2 cut(s) 526, 1082
Cfr13I GGNCC 3 cut(s) 205, 310, 689
Csp6I GTAC 2 cut(s) 278, 923
CviQI GTAC 2 cut(s) 278, 923
DdeI CTNAG 2 cut(s) 9, 941
DpnI GATC 5 cut(s) 390, 579, 811, 910, 961
DpnII GATC 5 cut(s) 388, 577, 809, 908, 959
DraI TTTAAA 1 cut(s) 658
EaeI YGGCCR 2 cut(s) 452, 469
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
Ecl136II GAGCTC 1 cut(s) 8
Eco130I CCWWGG 2 cut(s) 313, 324
Eco24I GRGCYC 1 cut(s) 10
Eco31I GGTCTC 1 cut(s) 480
Eco47I GGWCC 1 cut(s) 310
Eco53kI GAGCTC 1 cut(s) 8
Eco81I CCTNAGG 1 cut(s) 941
Eco88I CYCGRG 1 cut(s) 299
EcoICRI GAGCTC 1 cut(s) 8
EcoNI CCTNNNNNAGG 1 cut(s) 238
EcoO109I RGGNCCY 1 cut(s) 205
EcoRII CCWGG 2 cut(s) 925, 955
EcoT14I CCWWGG 2 cut(s) 313, 324
EcoT38I GRGCYC 1 cut(s) 10
ErhI CCWWGG 2 cut(s) 313, 324
FbaI TGATCA 1 cut(s) 577
Fnu4HI GCNGC 1 cut(s) 472
FokI GGATG 2 cut(s) 5, 141
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 1 cut(s) 472
FspBI CTAG 3 cut(s) 260, 429, 947
GluI GCNGC 1 cut(s) 472
GsaI CCCAGC 2 cut(s) 695, 938
HaeIII GGCC 6 cut(s) 207, 233, 244, 454, 471, 690
HapII CCGG 2 cut(s) 340, 451
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 4 cut(s) 139, 215, 554, 678
HpaI GTTAAC 1 cut(s) 49
HpaII CCGG 2 cut(s) 340, 451
HphI GGTGA 2 cut(s) 288, 806
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 1 cut(s) 12
Hpy188III TCNNGA 6 cut(s) 369, 527, 703, 813, 963, 1083
Hpy8I GTNNAC 1 cut(s) 49
HpyAV CCTTC 3 cut(s) 234, 810, 866
HpyCH4III ACNGT 2 cut(s) 270, 1002
HpyCH4IV ACGT 1 cut(s) 51
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 429, 451, 480, 774, 1034
HpyF3I CTNAG 2 cut(s) 9, 941
HpySE526I ACGT 1 cut(s) 51
Ksp22I TGATCA 1 cut(s) 577
KspAI GTTAAC 1 cut(s) 49
Kzo9I GATC 5 cut(s) 388, 577, 809, 908, 959
LguI GCTCTTC 1 cut(s) 8
LmnI GCTCC 2 cut(s) 5, 748
LweI GCATC 5 cut(s) 27, 75, 677, 855, 1074
MaeI CTAG 3 cut(s) 260, 429, 947
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 1 cut(s) 505
MalI GATC 5 cut(s) 390, 579, 811, 910, 961
MbiI CCGCTC 1 cut(s) 474
MboI GATC 5 cut(s) 388, 577, 809, 908, 959
MboII GAAGA 2 cut(s) 25, 977
MfeI CAATTG 2 cut(s) 726, 762
MflI RGATCY 3 cut(s) 388, 908, 959
MhlI GDGCHC 1 cut(s) 10
MluCI AATT 6 cut(s) 331, 361, 659, 726, 762, 842
MlyI GAGTC 2 cut(s) 148, 687
MnlI CCTC 9 cut(s) 88, 102, 129, 347, 534, 710, 779, 790, 936
MseI TTAA 7 cut(s) 48, 150, 648, 657, 845, 858, 1032
MslI CAYNNNNRTG 1 cut(s) 323
MspI CCGG 2 cut(s) 340, 451
MspR9I CCNGG 3 cut(s) 340, 927, 957
MunI CAATTG 2 cut(s) 726, 762
Mva1269I GAATGC 3 cut(s) 286, 318, 695
MvaI CCWGG 2 cut(s) 927, 957
MwoI GCNNNNNNNGC 6 cut(s) 32, 429, 451, 480, 774, 1034
NciI CCSGG 1 cut(s) 340
NcoI CCATGG 2 cut(s) 313, 324
NdeII GATC 5 cut(s) 388, 577, 809, 908, 959
NheI GCTAGC 1 cut(s) 259
NlaIV GGNNCC 2 cut(s) 311, 744
NmeAIII GCCGAG 1 cut(s) 509
NmuCI GTSAC 1 cut(s) 505
NspI RCATGY 3 cut(s) 449, 513, 825
PagI TCATGA 2 cut(s) 526, 1082
PciSI GCTCTTC 1 cut(s) 8
PctI GAATGC 3 cut(s) 286, 318, 695
PfeI GAWTC 2 cut(s) 215, 554
PflMI CCANNNNNTGG 1 cut(s) 962
PkrI GCNGC 1 cut(s) 473
PleI GAGTC 2 cut(s) 147, 686
PpsI GAGTC 2 cut(s) 147, 686
PshBI ATTAAT 1 cut(s) 1032
Psp124BI GAGCTC 1 cut(s) 10
Psp1406I AACGTT 1 cut(s) 51
Psp6I CCWGG 2 cut(s) 925, 955
PspFI CCCAGC 2 cut(s) 691, 934
PspGI CCWGG 2 cut(s) 925, 955
PspN4I GGNNCC 2 cut(s) 311, 744
PspPI GGNCC 3 cut(s) 205, 310, 689
PsrI GAACNNNNNNTAC 2 cut(s) 603, 635
PstNI CAGNNNCTG 1 cut(s) 962
PsuI RGATCY 3 cut(s) 388, 908, 959
RsaI GTAC 2 cut(s) 279, 924
RsaNI GTAC 2 cut(s) 278, 923
RseI CAYNNNNRTG 1 cut(s) 323
SacI GAGCTC 1 cut(s) 10
SapI GCTCTTC 1 cut(s) 8
SaqAI TTAA 7 cut(s) 48, 150, 648, 657, 845, 858, 1032
SatI GCNGC 1 cut(s) 472
Sau3AI GATC 5 cut(s) 388, 577, 809, 908, 959
Sau96I GGNCC 3 cut(s) 205, 310, 689
SchI GAGTC 2 cut(s) 148, 687
ScrFI CCNGG 3 cut(s) 340, 927, 957
SduI GDGCHC 1 cut(s) 10
SfaNI GCATC 5 cut(s) 27, 75, 677, 855, 1074
SfcI CTRYAG 2 cut(s) 235, 281
SinI GGWCC 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 323
Sse9I AATT 6 cut(s) 331, 361, 659, 726, 762, 842
SsiI CCGC 2 cut(s) 472, 768
SspI AATATT 1 cut(s) 645
SspMI CTAG 3 cut(s) 260, 429, 947
SstI GAGCTC 1 cut(s) 10
StyD4I CCNGG 3 cut(s) 338, 925, 955
StyI CCWWGG 2 cut(s) 313, 324
TaaI ACNGT 2 cut(s) 270, 1002
TaiI ACGT 1 cut(s) 54
TaqI TCGA 1 cut(s) 370
TasI AATT 6 cut(s) 331, 361, 659, 726, 762, 842
TatI WGTACW 1 cut(s) 277
TauI GCSGC 1 cut(s) 474
TfiI GAWTC 2 cut(s) 215, 554
Tru1I TTAA 7 cut(s) 48, 150, 648, 657, 845, 858, 1032
Tru9I TTAA 7 cut(s) 48, 150, 648, 657, 845, 858, 1032
TscAI CASTG 4 cut(s) 115, 462, 520, 784
TseFI GTSAC 1 cut(s) 505
Tsp45I GTSAC 1 cut(s) 505
TspDTI ATGAA 2 cut(s) 581, 1071
TspRI CASTG 4 cut(s) 115, 462, 520, 784
Van91I CCANNNNNTGG 1 cut(s) 962
VpaK11BI GGWCC 1 cut(s) 310
VspI ATTAAT 1 cut(s) 1032
XagI CCTNNNNNAGG 1 cut(s) 238
XapI RAATTY 2 cut(s) 361, 659
XceI RCATGY 3 cut(s) 449, 513, 825
XcmI CCANNNNNNNNNTGG 1 cut(s) 252
XspI CTAG 3 cut(s) 260, 429, 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.