Rw5G030170

Outer envelope protein 80

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0015354
Physical Location & Seq
Reverse (-)
47540509 .. 47545765
5257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G030170.1

Sequence Viewer

Length: 1086 bp
ATGGGAGCTCAGAAGAGCATCCATGCTGGCAAAGCCAAGATTGATGTTAACGTTGATTTCACTCACAAGCTTTGTGCCTCTTTGATGCTTCCTCCTTTGAGTAATGGCAGTGGCAGTCCTCTCTCTCTCATCATCGGGAGTCTTTGCATCAAACATCCAAACTTGTTTGGTGGAAGTGAGAAGCTTGATGTGTCATTGGATAAGGGCCTGTATGATTCGAATGTCTTATTGGCTTATAGAAGGCCAAGACCTCAGTGGCTAGCTCAACAGTCTTTTGTACTACAGCATTCTATTTCACCCGAGATAGGGGTCCATGGCATTCCCATGGACAATTTCTCCCGGTCAGCTAGTGGAGGTGTAAATTTGTCTCGATTATCAGTAGGCATGGATCTCAATGAGCCTGCAAGTTCCAAGTGGAGTAGCACAACTAGCCTAAAGTTTGAGCATGTCCGGCCAATGGATGATAATGGCCGCTCAATGAGCCGAGATCTTGATGGGTTTCATGTCACATGCAGTGGAGGTCCTCATGATAGTATGGTAGTTCTAAAGCAAGAATCTCGGTATGCAAGGGCAACTGATCGCAGTTTTTTTCAGTTCAGTCTGCAAATAGAACAAGGGATACCAGTTCTATCAAAGTGGCTAATATTTAACAAGTTTAAATTCATTGCATCAAAGGGAGTCAAACTTGGGCCAGCATTCCTCTTGACAAGAATGACAGGCGGTTCCATTGTGGGGGACATGGCTCCTTACCAAGCATTTGCAATTGGCGGTCTTGGCAGTGTGCGAGGGTATGGTGAGGGTGCAGTGGGATCGGGGAGGACATGCCTGGTTGCAAATAGTGAATTGACATTACCTTTTACCAAGATGCTGGAAGGTGCTGTTTTCTTGGATTGTGGAACTGATTTGGGTTCTGCTCATCATGTGCCTGGAAACCCAGCCCTGAGGCAAGGGAAACCAGGATCTGGAGTAGGGCTTGGATATGGTCTTCGTTTGAAATCACAGTTTGGTCATTTTGTGGTTGATTACGCCATTAATGCCTTCCAACAAAAAACAGTCTATTTTGGCATCAGCAACCTTGCTTCATGA

Protein Analysis

361

Amino Acids

38.71

Weight (kDa)

9.31

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Omp85 PF01103 54 - 357 4.1e-30 Omp85 superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44160
fragaria_vesca FvH4_3g26270
malus_domestica MD11G1174200.v1.1
prunus_persica Prupe.6G146000_v2.0.a1
pyrus_communis pycom11g14900
rosa_chinensis RchiOBHm_Chr5g0048701
rosa_laevigata RLG00000034550
rosa_multiflora Rmu_sc0006432.1_g000004
rosa_roxburghii Rroxscaffold_1G00033100
rosa_rugosa Rorug05G0240700
rosa_samantha Rh5AG320500 Rh5BG330200 Rh5CG356200 Rh5DG341400
rosa_wichuraiana Rw5G030170 Rw5G030260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 962
AccBSI CCGCTC 1 cut(s) 474
AciI CCGC 3 cut(s) 472, 720, 768
AclI AACGTT 1 cut(s) 51
AclWI GGATC 3 cut(s) 396, 817, 967
AcoI YGGCCR 2 cut(s) 452, 469
AcsI RAATTY 2 cut(s) 361, 659
AfaI GTAC 1 cut(s) 279
AfiI CCNNNNNNNGG 5 cut(s) 305, 306, 457, 732, 962
AgsI TTSAA 1 cut(s) 994
AjnI CCWGG 3 cut(s) 825, 925, 955
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
AluBI AGCT 5 cut(s) 8, 70, 184, 263, 347
AluI AGCT 5 cut(s) 8, 70, 184, 263, 347
Alw21I GWGCWC 1 cut(s) 10
Alw26I GTCTC 1 cut(s) 372
AlwI GGATC 3 cut(s) 396, 817, 967
AlwNI CAGNNNCTG 1 cut(s) 962
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 5 cut(s) 205, 242, 452, 469, 689
ApoI RAATTY 2 cut(s) 361, 659
AseI ATTAAT 1 cut(s) 1032
AspS9I GGNCC 4 cut(s) 205, 310, 521, 689
AsuC2I CCSGG 1 cut(s) 340
AsuHPI GGTGA 2 cut(s) 288, 806
AsuII TTCGAA 1 cut(s) 218
AsuNHI GCTAGC 1 cut(s) 259
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 2 cut(s) 310, 521
AxyI CCTNAGG 1 cut(s) 941
BanII GRGCYC 1 cut(s) 10
BbsI GAAGAC 1 cut(s) 977
Bbv12I GWGCWC 1 cut(s) 10
BccI CCATC 1 cut(s) 488
BcgI CGANNNNNNTGC 4 cut(s) 539, 573, 792, 826
BciT130I CCWGG 3 cut(s) 827, 927, 957
BciVI GTATCC 1 cut(s) 612
BcnI CCSGG 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 372
BfaI CTAG 3 cut(s) 260, 348, 429
BfmI CTRYAG 1 cut(s) 281
BfuI GTATCC 1 cut(s) 612
BglII AGATCT 1 cut(s) 487
BisI GCNGC 1 cut(s) 472
BlsI GCNGC 1 cut(s) 473
Bme1390I CCNGG 4 cut(s) 340, 827, 927, 957
Bme18I GGWCC 2 cut(s) 310, 521
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 4 cut(s) 205, 310, 521, 689
BmiI GGNNCC 3 cut(s) 311, 724, 744
BmrFI CCNGG 4 cut(s) 340, 827, 927, 957
BmsI GCATC 6 cut(s) 27, 75, 156, 677, 855, 1074
BmtI GCTAGC 1 cut(s) 263
BpiI GAAGAC 1 cut(s) 977
BpmI CTGGAG 1 cut(s) 984
Bpu14I TTCGAA 1 cut(s) 218
BpuMI CCSGG 1 cut(s) 340
BsaJI CCNNGG 2 cut(s) 313, 324
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 5 cut(s) 305, 306, 457, 732, 962
Bse1I ACTGG 1 cut(s) 623
Bse21I CCTNAGG 1 cut(s) 941
Bse3DI GCAATG 1 cut(s) 663
BseBI CCWGG 3 cut(s) 827, 927, 957
BseDI CCNNGG 2 cut(s) 313, 324
BseGI GGATG 3 cut(s) 18, 154, 466
BseLI CCNNNNNNNGG 5 cut(s) 305, 306, 457, 732, 962
BseMI GCAATG 1 cut(s) 663
BseMII CTCAG 3 cut(s) 23, 266, 932
BseNI ACTGG 1 cut(s) 623
BseYI CCCAGC 1 cut(s) 934
BsgI GTGCAG 1 cut(s) 822
BshFI GGCC 5 cut(s) 207, 244, 454, 471, 691
BsiHKAI GWGCWC 1 cut(s) 10
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 2 cut(s) 340, 451
BslFI GGGAC 1 cut(s) 749
BslI CCNNNNNNNGG 5 cut(s) 305, 306, 457, 732, 962
BsmAI GTCTC 1 cut(s) 372
BsmFI GGGAC 1 cut(s) 749
BsmI GAATGC 3 cut(s) 286, 318, 695
BsnI GGCC 5 cut(s) 207, 244, 454, 471, 691
BsoBI CYCGRG 1 cut(s) 299
Bsp119I TTCGAA 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 10
Bsp143I GATC 5 cut(s) 388, 487, 577, 809, 959
Bsp19I CCATGG 2 cut(s) 313, 324
BspACI CCGC 3 cut(s) 472, 720, 768
BspANI GGCC 5 cut(s) 207, 244, 454, 471, 691
BspCNI CTCAG 3 cut(s) 22, 265, 933
BspHI TCATGA 2 cut(s) 526, 1082
BspLI GGNNCC 3 cut(s) 311, 724, 744
BspOI GCTAGC 1 cut(s) 263
BspPI GGATC 3 cut(s) 396, 817, 967
BspQI GCTCTTC 1 cut(s) 8
BspT104I TTCGAA 1 cut(s) 218
BsrBI CCGCTC 1 cut(s) 474
BsrDI GCAATG 1 cut(s) 663
BsrI ACTGG 1 cut(s) 623
BssECI CCNNGG 2 cut(s) 313, 324
BssMI GATC 5 cut(s) 388, 487, 577, 809, 959
BssT1I CCWWGG 2 cut(s) 313, 324
Bst2UI CCWGG 3 cut(s) 827, 927, 957
Bst4CI ACNGT 3 cut(s) 270, 1002, 1054
Bst6I CTCTTC 1 cut(s) 8
BstBI TTCGAA 1 cut(s) 218
BstC8I GCNNGC 4 cut(s) 28, 261, 402, 693
BstDEI CTNAG 3 cut(s) 9, 252, 941
BstDSI CCRYGG 2 cut(s) 313, 324
BstF5I GGATG 3 cut(s) 18, 154, 466
BstKTI GATC 5 cut(s) 391, 490, 580, 812, 962
BstMAI GTCTC 1 cut(s) 372
BstMBI GATC 5 cut(s) 388, 487, 577, 809, 959
BstMWI GCNNNNNNNGC 6 cut(s) 32, 429, 451, 480, 774, 1034
BstNI CCWGG 3 cut(s) 827, 927, 957
BstNSI RCATGY 3 cut(s) 449, 513, 825
BstSCI CCNGG 4 cut(s) 338, 825, 925, 955
BstSFI CTRYAG 1 cut(s) 281
BstV2I GAAGAC 1 cut(s) 977
BstX2I RGATCY 3 cut(s) 388, 487, 959
BstXI CCANNNNNNTGG 1 cut(s) 868
BstYI RGATCY 3 cut(s) 388, 487, 959
Bsu36I CCTNAGG 1 cut(s) 941
BsuI GTATCC 1 cut(s) 612
BsuRI GGCC 5 cut(s) 207, 244, 454, 471, 691
BtgI CCRYGG 2 cut(s) 313, 324
BtsCI GGATG 3 cut(s) 18, 154, 466
BtsI GCAGTG 4 cut(s) 115, 520, 784, 810
BtsIMutI CAGTG 5 cut(s) 115, 260, 520, 784, 810
Cac8I GCNNGC 4 cut(s) 28, 261, 402, 693
CaiI CAGNNNCTG 1 cut(s) 962
CciI TCATGA 2 cut(s) 526, 1082
Cfr13I GGNCC 4 cut(s) 205, 310, 521, 689
Csp6I GTAC 1 cut(s) 278
CviQI GTAC 1 cut(s) 278
DdeI CTNAG 3 cut(s) 9, 252, 941
DpnI GATC 5 cut(s) 390, 489, 579, 811, 961
DpnII GATC 5 cut(s) 388, 487, 577, 809, 959
DraI TTTAAA 1 cut(s) 658
EaeI YGGCCR 2 cut(s) 452, 469
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
Ecl136II GAGCTC 1 cut(s) 8
Eco130I CCWWGG 2 cut(s) 313, 324
Eco24I GRGCYC 1 cut(s) 10
Eco47I GGWCC 2 cut(s) 310, 521
Eco53kI GAGCTC 1 cut(s) 8
Eco81I CCTNAGG 1 cut(s) 941
Eco88I CYCGRG 1 cut(s) 299
EcoICRI GAGCTC 1 cut(s) 8
EcoO109I RGGNCCY 2 cut(s) 205, 521
EcoRII CCWGG 3 cut(s) 825, 925, 955
EcoT14I CCWWGG 2 cut(s) 313, 324
EcoT38I GRGCYC 1 cut(s) 10
ErhI CCWWGG 2 cut(s) 313, 324
FaqI GGGAC 1 cut(s) 749
Fnu4HI GCNGC 1 cut(s) 472
FokI GGATG 3 cut(s) 5, 141, 473
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 1 cut(s) 472
FspBI CTAG 3 cut(s) 260, 348, 429
GluI GCNGC 1 cut(s) 472
GsaI CCCAGC 1 cut(s) 938
GsuI CTGGAG 1 cut(s) 984
HaeIII GGCC 5 cut(s) 207, 244, 454, 471, 691
HapII CCGG 2 cut(s) 340, 451
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HindIII AAGCTT 2 cut(s) 68, 182
HinfI GANTC 4 cut(s) 139, 215, 554, 678
HpaI GTTAAC 1 cut(s) 49
HpaII CCGG 2 cut(s) 340, 451
HphI GGTGA 2 cut(s) 288, 806
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 1 cut(s) 12
Hpy188III TCNNGA 7 cut(s) 136, 369, 491, 527, 703, 963, 1083
Hpy8I GTNNAC 1 cut(s) 49
HpyAV CCTTC 3 cut(s) 234, 866, 1048
HpyCH4III ACNGT 3 cut(s) 270, 1002, 1054
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 9 cut(s) 147, 404, 513, 566, 604, 668, 761, 803, 833
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 429, 451, 480, 774, 1034
HpyF3I CTNAG 3 cut(s) 9, 252, 941
HpySE526I ACGT 1 cut(s) 51
KspAI GTTAAC 1 cut(s) 49
Kzo9I GATC 5 cut(s) 388, 487, 577, 809, 959
LguI GCTCTTC 1 cut(s) 8
LmnI GCTCC 2 cut(s) 5, 748
LweI GCATC 6 cut(s) 27, 75, 156, 677, 855, 1074
MaeI CTAG 3 cut(s) 260, 348, 429
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 1 cut(s) 505
MalI GATC 5 cut(s) 390, 489, 579, 811, 961
MbiI CCGCTC 1 cut(s) 474
MboI GATC 5 cut(s) 388, 487, 577, 809, 959
MboII GAAGA 2 cut(s) 25, 977
MfeI CAATTG 1 cut(s) 762
MflI RGATCY 3 cut(s) 388, 487, 959
MhlI GDGCHC 1 cut(s) 10
MluCI AATT 5 cut(s) 331, 361, 659, 762, 842
MlyI GAGTC 2 cut(s) 148, 687
MmeI TCCRAC 1 cut(s) 1066
MseI TTAA 4 cut(s) 48, 648, 657, 1032
MslI CAYNNNNRTG 1 cut(s) 323
MspI CCGG 2 cut(s) 340, 451
MspR9I CCNGG 4 cut(s) 340, 827, 927, 957
MunI CAATTG 1 cut(s) 762
Mva1269I GAATGC 3 cut(s) 286, 318, 695
MvaI CCWGG 3 cut(s) 827, 927, 957
MwoI GCNNNNNNNGC 6 cut(s) 32, 429, 451, 480, 774, 1034
NciI CCSGG 1 cut(s) 340
NcoI CCATGG 2 cut(s) 313, 324
NdeII GATC 5 cut(s) 388, 487, 577, 809, 959
NheI GCTAGC 1 cut(s) 259
NlaIV GGNNCC 3 cut(s) 311, 724, 744
NmeAIII GCCGAG 1 cut(s) 509
NmuCI GTSAC 1 cut(s) 505
NspI RCATGY 3 cut(s) 449, 513, 825
NspV TTCGAA 1 cut(s) 218
PagI TCATGA 2 cut(s) 526, 1082
PciSI GCTCTTC 1 cut(s) 8
PctI GAATGC 3 cut(s) 286, 318, 695
PfeI GAWTC 2 cut(s) 215, 554
PflMI CCANNNNNTGG 1 cut(s) 962
PkrI GCNGC 1 cut(s) 473
PleI GAGTC 2 cut(s) 147, 686
PpsI GAGTC 2 cut(s) 147, 686
PpuMI RGGWCCY 1 cut(s) 521
PshBI ATTAAT 1 cut(s) 1032
Psp124BI GAGCTC 1 cut(s) 10
Psp1406I AACGTT 1 cut(s) 51
Psp5II RGGWCCY 1 cut(s) 521
Psp6I CCWGG 3 cut(s) 825, 925, 955
PspFI CCCAGC 1 cut(s) 934
PspGI CCWGG 3 cut(s) 825, 925, 955
PspN4I GGNNCC 3 cut(s) 311, 724, 744
PspPI GGNCC 4 cut(s) 205, 310, 521, 689
PspPPI RGGWCCY 1 cut(s) 521
PsrI GAACNNNNNNTAC 2 cut(s) 603, 635
PstNI CAGNNNCTG 1 cut(s) 962
PsuI RGATCY 3 cut(s) 388, 487, 959
RsaI GTAC 1 cut(s) 279
RsaNI GTAC 1 cut(s) 278
RseI CAYNNNNRTG 1 cut(s) 323
SacI GAGCTC 1 cut(s) 10
SapI GCTCTTC 1 cut(s) 8
SaqAI TTAA 4 cut(s) 48, 648, 657, 1032
SatI GCNGC 1 cut(s) 472
Sau3AI GATC 5 cut(s) 388, 487, 577, 809, 959
Sau96I GGNCC 4 cut(s) 205, 310, 521, 689
SchI GAGTC 2 cut(s) 148, 687
ScrFI CCNGG 4 cut(s) 340, 827, 927, 957
SduI GDGCHC 1 cut(s) 10
SfaNI GCATC 6 cut(s) 27, 75, 156, 677, 855, 1074
SfcI CTRYAG 1 cut(s) 281
SfuI TTCGAA 1 cut(s) 218
SinI GGWCC 2 cut(s) 310, 521
SmiMI CAYNNNNRTG 1 cut(s) 323
Sse9I AATT 5 cut(s) 331, 361, 659, 762, 842
SsiI CCGC 3 cut(s) 472, 720, 768
SspI AATATT 1 cut(s) 645
SspMI CTAG 3 cut(s) 260, 348, 429
SstI GAGCTC 1 cut(s) 10
StyD4I CCNGG 4 cut(s) 338, 825, 925, 955
StyI CCWWGG 2 cut(s) 313, 324
TaaI ACNGT 3 cut(s) 270, 1002, 1054
TaiI ACGT 1 cut(s) 54
TaqI TCGA 2 cut(s) 218, 370
TasI AATT 5 cut(s) 331, 361, 659, 762, 842
TatI WGTACW 1 cut(s) 277
TauI GCSGC 1 cut(s) 474
TfiI GAWTC 2 cut(s) 215, 554
Tru1I TTAA 4 cut(s) 48, 648, 657, 1032
Tru9I TTAA 4 cut(s) 48, 648, 657, 1032
TscAI CASTG 5 cut(s) 115, 260, 520, 784, 810
TseFI GTSAC 1 cut(s) 505
Tsp45I GTSAC 1 cut(s) 505
TspDTI ATGAA 3 cut(s) 491, 652, 1071
TspRI CASTG 5 cut(s) 115, 260, 520, 784, 810
Van91I CCANNNNNTGG 1 cut(s) 962
VpaK11BI GGWCC 2 cut(s) 310, 521
VspI ATTAAT 1 cut(s) 1032
XapI RAATTY 2 cut(s) 361, 659
XceI RCATGY 3 cut(s) 449, 513, 825
XcmI CCANNNNNNNNNTGG 1 cut(s) 252
XspI CTAG 3 cut(s) 260, 348, 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.