Rh5BG330200

Outer envelope protein 80

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0015354
Physical Location & Seq
Forward (+)
45755299 .. 45760572
5274 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG330200.1

Sequence Viewer

Length: 936 bp
ATGGGAGCTCAGAAGAGCATCCATGCTGGCAAAGCCAAGATTGATGTTAACGTTGATTTCACTCACAAGCTTTGTGCCTCTTTGATGCTTCCTCCTTTGAGTAATGGCAGTGGCAGTCCCCTCTCTCTCATCATCGGGAGTCTTTGCATCAAACATCCAAACTTGTTTGGTGGAAGTGAGAAGCTTGATGTGTCATTGGATAAGGGCCTGTATGACTCGAATGTCTTATTGGCTTATAGAAGGCCAAGACCTCAATGGCTAGCTCAACAGTCTTTTGTACTACAGCACTCTATTTCACCTGAGATAGGGGTCCATGGCATTCCCATGGACAATTTCTCCCGGTCAGCTAGTGGAGGTGTAAATTTGTCTCGATTATCAGTAGGCATGGATCTCAATGAGCCTGCAAGTTCGAAGTGGAGTAGCACAACTAGCCTAAAGTTTGAGCATGTCCGGCCAATGGATGATAATGGCCGCTCAATGAGCCGAGATCTTGATGGGTTTCCTGTCACATGCAGTGGAAGTCCTCATGATAGTATGGTAGTTCTAAAGCAAGAATCTCGGTATGCAAGGGCAACTGATCGCAGTTTTTTTCAGTTCAGTCTGCAAATAGAACAAGGGATACCAGTTCTATCAAAGTGGCTAATATTTAACAAGTTTAAATTCATTGCATCAAAGGGAGTCAAACTTGGGCCAGCATTCCTCTTGACAAGAATGACAGGCGGTTCCATTGTGGGGGACATGGCTCCTTACCAAGCATTTGCCATTGGCGGTCTTGGCAGTGTGCGAGGGTATGGTGAGGGTGCAGTGGGATCGGGGAGGACATGCCTGGTTGCAAATAGCGAATTGACATTACCTTTTGTATGTCATACCCCTTTTTGTTTCTGCGTTGCCTCAATTGCTAAGAATTATATTCCATTTATTGCATTATTTTATTAG

Protein Analysis

311

Amino Acids

33.66

Weight (kDa)

9.15

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Omp85 PF01103 54 - 286 1.6e-15 Omp85 superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44160
fragaria_vesca FvH4_3g26270
malus_domestica MD11G1174200.v1.1
prunus_persica Prupe.6G146000_v2.0.a1
pyrus_communis pycom11g14900
rosa_chinensis RchiOBHm_Chr5g0048701
rosa_laevigata RLG00000034550
rosa_multiflora Rmu_sc0006432.1_g000004
rosa_roxburghii Rroxscaffold_1G00033100
rosa_rugosa Rorug05G0240700
rosa_samantha Rh5AG320500 Rh5BG330200 Rh5CG356200 Rh5DG341400
rosa_wichuraiana Rw5G030170 Rw5G030260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 221
AccBSI CCGCTC 1 cut(s) 474
AciI CCGC 3 cut(s) 472, 720, 768
AclI AACGTT 1 cut(s) 51
AclWI GGATC 2 cut(s) 396, 817
AcoI YGGCCR 2 cut(s) 452, 469
AcsI RAATTY 2 cut(s) 361, 659
AfaI GTAC 1 cut(s) 279
AfiI CCNNNNNNNGG 3 cut(s) 305, 457, 732
AjnI CCWGG 1 cut(s) 825
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
AluBI AGCT 5 cut(s) 8, 70, 184, 263, 347
AluI AGCT 5 cut(s) 8, 70, 184, 263, 347
Alw21I GWGCWC 1 cut(s) 10
Alw26I GTCTC 1 cut(s) 372
AlwI GGATC 2 cut(s) 396, 817
AoxI GGCC 5 cut(s) 205, 242, 452, 469, 689
ApoI RAATTY 2 cut(s) 361, 659
AspS9I GGNCC 3 cut(s) 205, 310, 689
AsuC2I CCSGG 1 cut(s) 340
AsuHPI GGTGA 2 cut(s) 288, 806
AsuII TTCGAA 1 cut(s) 410
AsuNHI GCTAGC 1 cut(s) 259
AvaII GGWCC 1 cut(s) 310
BanII GRGCYC 1 cut(s) 10
Bbv12I GWGCWC 1 cut(s) 10
BccI CCATC 1 cut(s) 488
BcgI CGANNNNNNTGC 4 cut(s) 539, 573, 792, 826
BciT130I CCWGG 1 cut(s) 827
BciVI GTATCC 1 cut(s) 612
BcnI CCSGG 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 372
BfaI CTAG 3 cut(s) 260, 348, 429
BfmI CTRYAG 1 cut(s) 281
BfuI GTATCC 1 cut(s) 612
BglII AGATCT 1 cut(s) 487
BisI GCNGC 1 cut(s) 472
BlsI GCNGC 1 cut(s) 473
Bme1390I CCNGG 2 cut(s) 340, 827
Bme18I GGWCC 1 cut(s) 310
BmgT120I GGNCC 3 cut(s) 205, 310, 689
BmiI GGNNCC 3 cut(s) 311, 724, 744
BmrFI CCNGG 2 cut(s) 340, 827
BmsI GCATC 4 cut(s) 27, 75, 156, 677
BmtI GCTAGC 1 cut(s) 263
Bpu14I TTCGAA 1 cut(s) 410
BpuMI CCSGG 1 cut(s) 340
BsaJI CCNNGG 2 cut(s) 313, 324
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 3 cut(s) 305, 457, 732
Bse1I ACTGG 1 cut(s) 623
Bse3DI GCAATG 1 cut(s) 663
BseBI CCWGG 1 cut(s) 827
BseDI CCNNGG 2 cut(s) 313, 324
BseGI GGATG 3 cut(s) 18, 154, 466
BseLI CCNNNNNNNGG 3 cut(s) 305, 457, 732
BseMI GCAATG 1 cut(s) 663
BseMII CTCAG 2 cut(s) 23, 291
BseNI ACTGG 1 cut(s) 623
BsgI GTGCAG 1 cut(s) 822
BshFI GGCC 5 cut(s) 207, 244, 454, 471, 691
BsiHKAI GWGCWC 1 cut(s) 10
BsiSI CCGG 2 cut(s) 340, 451
BslFI GGGAC 2 cut(s) 102, 749
BslI CCNNNNNNNGG 3 cut(s) 305, 457, 732
BsmAI GTCTC 1 cut(s) 372
BsmFI GGGAC 2 cut(s) 102, 749
BsmI GAATGC 2 cut(s) 318, 695
BsnI GGCC 5 cut(s) 207, 244, 454, 471, 691
Bsp119I TTCGAA 1 cut(s) 410
Bsp1286I GDGCHC 1 cut(s) 10
Bsp143I GATC 4 cut(s) 388, 487, 577, 809
Bsp19I CCATGG 2 cut(s) 313, 324
BspACI CCGC 3 cut(s) 472, 720, 768
BspANI GGCC 5 cut(s) 207, 244, 454, 471, 691
BspCNI CTCAG 2 cut(s) 22, 292
BspHI TCATGA 1 cut(s) 526
BspLI GGNNCC 3 cut(s) 311, 724, 744
BspOI GCTAGC 1 cut(s) 263
BspPI GGATC 2 cut(s) 396, 817
BspQI GCTCTTC 1 cut(s) 8
BspT104I TTCGAA 1 cut(s) 410
BsrBI CCGCTC 1 cut(s) 474
BsrDI GCAATG 1 cut(s) 663
BsrI ACTGG 1 cut(s) 623
BssECI CCNNGG 2 cut(s) 313, 324
BssMI GATC 4 cut(s) 388, 487, 577, 809
BssT1I CCWWGG 2 cut(s) 313, 324
Bst2UI CCWGG 1 cut(s) 827
Bst4CI ACNGT 1 cut(s) 270
Bst6I CTCTTC 1 cut(s) 8
BstBI TTCGAA 1 cut(s) 410
BstC8I GCNNGC 4 cut(s) 28, 261, 402, 693
BstDEI CTNAG 3 cut(s) 9, 300, 900
BstDSI CCRYGG 2 cut(s) 313, 324
BstENI CCTNNNNNAGG 1 cut(s) 303
BstF5I GGATG 3 cut(s) 18, 154, 466
BstKTI GATC 4 cut(s) 391, 490, 580, 812
BstMAI GTCTC 1 cut(s) 372
BstMBI GATC 4 cut(s) 388, 487, 577, 809
BstMWI GCNNNNNNNGC 6 cut(s) 32, 429, 451, 480, 774, 896
BstNI CCWGG 1 cut(s) 827
BstNSI RCATGY 3 cut(s) 449, 513, 825
BstSCI CCNGG 2 cut(s) 338, 825
BstSFI CTRYAG 1 cut(s) 281
BstX2I RGATCY 2 cut(s) 388, 487
BstYI RGATCY 2 cut(s) 388, 487
BsuI GTATCC 1 cut(s) 612
BsuRI GGCC 5 cut(s) 207, 244, 454, 471, 691
BtgI CCRYGG 2 cut(s) 313, 324
BtsCI GGATG 3 cut(s) 18, 154, 466
BtsI GCAGTG 4 cut(s) 115, 520, 784, 810
BtsIMutI CAGTG 4 cut(s) 115, 520, 784, 810
Cac8I GCNNGC 4 cut(s) 28, 261, 402, 693
CciI TCATGA 1 cut(s) 526
Cfr13I GGNCC 3 cut(s) 205, 310, 689
Csp6I GTAC 1 cut(s) 278
CviAII CATG 9 cut(s) 23, 314, 325, 385, 446, 510, 527, 739, 822
CviQI GTAC 1 cut(s) 278
DdeI CTNAG 3 cut(s) 9, 300, 900
DpnI GATC 4 cut(s) 390, 489, 579, 811
DpnII GATC 4 cut(s) 388, 487, 577, 809
DraI TTTAAA 1 cut(s) 658
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 2 cut(s) 452, 469
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
Ecl136II GAGCTC 1 cut(s) 8
Eco130I CCWWGG 2 cut(s) 313, 324
Eco24I GRGCYC 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 310
Eco53kI GAGCTC 1 cut(s) 8
EcoICRI GAGCTC 1 cut(s) 8
EcoNI CCTNNNNNAGG 1 cut(s) 303
EcoO109I RGGNCCY 1 cut(s) 205
EcoRII CCWGG 1 cut(s) 825
EcoT14I CCWWGG 2 cut(s) 313, 324
EcoT38I GRGCYC 1 cut(s) 10
ErhI CCWWGG 2 cut(s) 313, 324
FaeI CATG 9 cut(s) 26, 317, 328, 388, 449, 513, 530, 742, 825
FaqI GGGAC 2 cut(s) 102, 749
FatI CATG 9 cut(s) 22, 313, 324, 384, 445, 509, 526, 738, 821
Fnu4HI GCNGC 1 cut(s) 472
FokI GGATG 3 cut(s) 5, 141, 473
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 1 cut(s) 472
FspBI CTAG 3 cut(s) 260, 348, 429
GluI GCNGC 1 cut(s) 472
HaeIII GGCC 5 cut(s) 207, 244, 454, 471, 691
HapII CCGG 2 cut(s) 340, 451
Hin1II CATG 9 cut(s) 26, 317, 328, 388, 449, 513, 530, 742, 825
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HindIII AAGCTT 2 cut(s) 68, 182
HinfI GANTC 4 cut(s) 139, 215, 554, 678
HpaI GTTAAC 1 cut(s) 49
HpaII CCGG 2 cut(s) 340, 451
HphI GGTGA 2 cut(s) 288, 806
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 1 cut(s) 12
Hpy188III TCNNGA 5 cut(s) 136, 369, 491, 527, 703
Hpy8I GTNNAC 1 cut(s) 49
HpyAV CCTTC 1 cut(s) 234
HpyCH4III ACNGT 1 cut(s) 270
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 9 cut(s) 147, 404, 513, 566, 604, 668, 803, 833, 923
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 429, 451, 480, 774, 896
HpyF3I CTNAG 3 cut(s) 9, 300, 900
HpySE526I ACGT 1 cut(s) 51
Hsp92II CATG 9 cut(s) 26, 317, 328, 388, 449, 513, 530, 742, 825
KspAI GTTAAC 1 cut(s) 49
Kzo9I GATC 4 cut(s) 388, 487, 577, 809
LguI GCTCTTC 1 cut(s) 8
LmnI GCTCC 2 cut(s) 5, 748
LweI GCATC 4 cut(s) 27, 75, 156, 677
MaeI CTAG 3 cut(s) 260, 348, 429
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 1 cut(s) 505
MalI GATC 4 cut(s) 390, 489, 579, 811
MbiI CCGCTC 1 cut(s) 474
MboI GATC 4 cut(s) 388, 487, 577, 809
MboII GAAGA 1 cut(s) 25
MfeI CAATTG 1 cut(s) 894
MflI RGATCY 2 cut(s) 388, 487
MhlI GDGCHC 1 cut(s) 10
MluCI AATT 6 cut(s) 331, 361, 659, 842, 894, 904
MlyI GAGTC 3 cut(s) 148, 209, 687
MseI TTAA 3 cut(s) 48, 648, 657
MslI CAYNNNNRTG 1 cut(s) 323
MspI CCGG 2 cut(s) 340, 451
MspR9I CCNGG 2 cut(s) 340, 827
MunI CAATTG 1 cut(s) 894
Mva1269I GAATGC 2 cut(s) 318, 695
MvaI CCWGG 1 cut(s) 827
MwoI GCNNNNNNNGC 6 cut(s) 32, 429, 451, 480, 774, 896
NciI CCSGG 1 cut(s) 340
NcoI CCATGG 2 cut(s) 313, 324
NdeII GATC 4 cut(s) 388, 487, 577, 809
NheI GCTAGC 1 cut(s) 259
NlaIII CATG 9 cut(s) 26, 317, 328, 388, 449, 513, 530, 742, 825
NlaIV GGNNCC 3 cut(s) 311, 724, 744
NmeAIII GCCGAG 1 cut(s) 509
NmuCI GTSAC 1 cut(s) 505
NspI RCATGY 3 cut(s) 449, 513, 825
NspV TTCGAA 1 cut(s) 410
PagI TCATGA 1 cut(s) 526
PciSI GCTCTTC 1 cut(s) 8
PctI GAATGC 2 cut(s) 318, 695
PfeI GAWTC 1 cut(s) 554
PkrI GCNGC 1 cut(s) 473
PleI GAGTC 3 cut(s) 147, 209, 686
PpsI GAGTC 3 cut(s) 147, 209, 686
Psp124BI GAGCTC 1 cut(s) 10
Psp1406I AACGTT 1 cut(s) 51
Psp6I CCWGG 1 cut(s) 825
PspGI CCWGG 1 cut(s) 825
PspN4I GGNNCC 3 cut(s) 311, 724, 744
PspPI GGNCC 3 cut(s) 205, 310, 689
PsrI GAACNNNNNNTAC 2 cut(s) 603, 635
PsuI RGATCY 2 cut(s) 388, 487
RsaI GTAC 1 cut(s) 279
RsaNI GTAC 1 cut(s) 278
RseI CAYNNNNRTG 1 cut(s) 323
SacI GAGCTC 1 cut(s) 10
SapI GCTCTTC 1 cut(s) 8
SaqAI TTAA 3 cut(s) 48, 648, 657
SatI GCNGC 1 cut(s) 472
Sau3AI GATC 4 cut(s) 388, 487, 577, 809
Sau96I GGNCC 3 cut(s) 205, 310, 689
SchI GAGTC 3 cut(s) 148, 209, 687
ScrFI CCNGG 2 cut(s) 340, 827
SduI GDGCHC 1 cut(s) 10
SfaNI GCATC 4 cut(s) 27, 75, 156, 677
SfcI CTRYAG 1 cut(s) 281
SfuI TTCGAA 1 cut(s) 410
SinI GGWCC 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 323
Sse9I AATT 6 cut(s) 331, 361, 659, 842, 894, 904
SsiI CCGC 3 cut(s) 472, 720, 768
SspI AATATT 1 cut(s) 645
SspMI CTAG 3 cut(s) 260, 348, 429
SstI GAGCTC 1 cut(s) 10
StyD4I CCNGG 2 cut(s) 338, 825
StyI CCWWGG 2 cut(s) 313, 324
TaaI ACNGT 1 cut(s) 270
TaiI ACGT 1 cut(s) 54
TaqI TCGA 3 cut(s) 218, 370, 410
TasI AATT 6 cut(s) 331, 361, 659, 842, 894, 904
TatI WGTACW 1 cut(s) 277
TauI GCSGC 1 cut(s) 474
TfiI GAWTC 1 cut(s) 554
Tru1I TTAA 3 cut(s) 48, 648, 657
Tru9I TTAA 3 cut(s) 48, 648, 657
TscAI CASTG 4 cut(s) 115, 520, 784, 810
TseFI GTSAC 1 cut(s) 505
Tsp45I GTSAC 1 cut(s) 505
TspDTI ATGAA 1 cut(s) 652
TspRI CASTG 4 cut(s) 115, 520, 784, 810
VpaK11BI GGWCC 1 cut(s) 310
XagI CCTNNNNNAGG 1 cut(s) 303
XapI RAATTY 2 cut(s) 361, 659
XceI RCATGY 3 cut(s) 449, 513, 825
XcmI CCANNNNNNNNNTGG 1 cut(s) 252
XspI CTAG 3 cut(s) 260, 348, 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.