Rh5AG320500

Outer envelope protein 80

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0015354
Physical Location & Seq
Forward (+)
47534388 .. 47539841
5454 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG320500.1

Sequence Viewer

Length: 1014 bp
ATGGGAGCTCAGAAGAGCATCCATGCTGGCAAAGCCAAGATTGATGTTAACGTTGATTTCACTCACAAGCTCTGTGCCTCTTTGATGCTTCCTCCTTTGAGTAATGGCAGTGGCAGTCCTCTCTCTCTCATCATCGGGAGTCTTTGCATCAAACATCCAAACTTGTTTGGTGGAAGTGAGAAGCTTGATGTGTCATTGGATAAGGGCCTGTATGATTCGAATGTCTTATTGGCTTATAGAAGGCCAAGACCTCAGTGGCTAGCTCAACAGTCTTTTGTACTACAGCATTCTATTTCACCCGAGATAGGGGTCCATGGCATTCCCATGGACAATTTCTCTCGGTCAGCTAGTGGAGGTGTAAATTTGTCTCGATTGTCAGTAGGCATGGATCTCAATGAGCCTGCAAGTTCCAAGTGGAGTAGCACAACTAGCCTAAAGTTTGAGCATGTCCGGCCAATGGATGATAATGGCCGCTCAATGAGCCGAGATCTTGATGGGTTTCCCGTCACATGCAGTGGAAGTCATCATGATAGTATGGTAGTTCTAAAGCAAGAATCTCGGTATGCAAAGGCAACTGATCGCAGTTTTTTTCAGTTCAGTCTGCAAATAGAACAAGGGATACCAGTTCTATCAAAGTGGCTAATATTTAACAAGTTTAAATTCATTGCATCAAAGGGAGTCAAACTTGGGCCAGCATTCCTCTTGACAAGAATGACAGGCGGTTCCATTGTGGGGGACATGGCTCCTTACCAAGCATTTGCAATTGGCGGTCTTGGCAGTGTGCGAGGGTATGGTGAGGGTGCAGTGGGATCGGGGAGGACATGCCTGGTTGCAAATAGTGAATTGACATTACCTTTTACCAAGATGCTGGAAGGTGCTGTTTTCTTGGATTGTGGAACTGATTTGGGTTCTGCTCATCATGTGCCAGGTGAGTCTCACTCTTTCATTTGGGTACATGAGTCTCACTCTTTCATTTGGGTACATGATATGCCTAGCTGCATCTTAGGTTTGTGA

Protein Analysis

337

Amino Acids

36.44

Weight (kDa)

8.19

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Omp85 PF01103 54 - 313 1.9e-21 Omp85 superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44160
fragaria_vesca FvH4_3g26270
malus_domestica MD11G1174200.v1.1
prunus_persica Prupe.6G146000_v2.0.a1
pyrus_communis pycom11g14900
rosa_chinensis RchiOBHm_Chr5g0048701
rosa_laevigata RLG00000034550
rosa_multiflora Rmu_sc0006432.1_g000004
rosa_roxburghii Rroxscaffold_1G00033100
rosa_rugosa Rorug05G0240700
rosa_samantha Rh5AG320500 Rh5BG330200 Rh5CG356200 Rh5DG341400
rosa_wichuraiana Rw5G030170 Rw5G030260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 474
AciI CCGC 3 cut(s) 472, 720, 768
AclI AACGTT 1 cut(s) 51
AclWI GGATC 2 cut(s) 396, 817
AcoI YGGCCR 2 cut(s) 452, 469
AcsI RAATTY 2 cut(s) 361, 659
AfaI GTAC 3 cut(s) 279, 954, 981
AfiI CCNNNNNNNGG 4 cut(s) 305, 306, 457, 732
AjnI CCWGG 2 cut(s) 825, 925
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
AluBI AGCT 6 cut(s) 8, 70, 184, 263, 347, 996
AluI AGCT 6 cut(s) 8, 70, 184, 263, 347, 996
Alw21I GWGCWC 1 cut(s) 10
Alw26I GTCTC 3 cut(s) 372, 939, 966
AlwI GGATC 2 cut(s) 396, 817
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 5 cut(s) 205, 242, 452, 469, 689
ApeKI GCWGC 1 cut(s) 996
ApoI RAATTY 2 cut(s) 361, 659
AspS9I GGNCC 3 cut(s) 205, 310, 689
AsuHPI GGTGA 3 cut(s) 288, 806, 941
AsuII TTCGAA 1 cut(s) 218
AsuNHI GCTAGC 1 cut(s) 259
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 1 cut(s) 310
BaeI ACNNNNGTAYC 2 cut(s) 944, 977
BanII GRGCYC 1 cut(s) 10
Bbv12I GWGCWC 1 cut(s) 10
BbvI GCAGC 1 cut(s) 983
BccI CCATC 1 cut(s) 488
BcgI CGANNNNNNTGC 4 cut(s) 539, 573, 792, 826
BciT130I CCWGG 2 cut(s) 827, 927
BciVI GTATCC 1 cut(s) 612
BcoDI GTCTC 3 cut(s) 372, 939, 966
BfaI CTAG 4 cut(s) 260, 348, 429, 993
BfmI CTRYAG 1 cut(s) 281
BfuI GTATCC 1 cut(s) 612
BglII AGATCT 1 cut(s) 487
BisI GCNGC 2 cut(s) 472, 997
BlsI GCNGC 2 cut(s) 473, 998
Bme1390I CCNGG 2 cut(s) 827, 927
Bme18I GGWCC 1 cut(s) 310
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 3 cut(s) 205, 310, 689
BmiI GGNNCC 3 cut(s) 311, 724, 744
BmrFI CCNGG 2 cut(s) 827, 927
BmsI GCATC 6 cut(s) 27, 75, 156, 677, 855, 1008
BmtI GCTAGC 1 cut(s) 263
BplI GAGNNNNNCTC 4 cut(s) 923, 955, 950, 982
Bpu14I TTCGAA 1 cut(s) 218
BsaJI CCNNGG 2 cut(s) 313, 324
Bsc4I CCNNNNNNNGG 4 cut(s) 305, 306, 457, 732
Bse1I ACTGG 1 cut(s) 623
Bse3DI GCAATG 1 cut(s) 663
BseBI CCWGG 2 cut(s) 827, 927
BseDI CCNNGG 2 cut(s) 313, 324
BseGI GGATG 3 cut(s) 18, 154, 466
BseLI CCNNNNNNNGG 4 cut(s) 305, 306, 457, 732
BseMI GCAATG 1 cut(s) 663
BseMII CTCAG 2 cut(s) 23, 266
BseNI ACTGG 1 cut(s) 623
BseXI GCAGC 1 cut(s) 983
BsgI GTGCAG 1 cut(s) 822
BshFI GGCC 5 cut(s) 207, 244, 454, 471, 691
BsiHKAI GWGCWC 1 cut(s) 10
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 1 cut(s) 451
BslFI GGGAC 1 cut(s) 749
BslI CCNNNNNNNGG 4 cut(s) 305, 306, 457, 732
BsmAI GTCTC 3 cut(s) 372, 939, 966
BsmFI GGGAC 1 cut(s) 749
BsmI GAATGC 3 cut(s) 286, 318, 695
BsnI GGCC 5 cut(s) 207, 244, 454, 471, 691
BsoBI CYCGRG 1 cut(s) 299
Bsp119I TTCGAA 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 10
Bsp143I GATC 4 cut(s) 388, 487, 577, 809
Bsp19I CCATGG 2 cut(s) 313, 324
BspACI CCGC 3 cut(s) 472, 720, 768
BspANI GGCC 5 cut(s) 207, 244, 454, 471, 691
BspCNI CTCAG 2 cut(s) 22, 265
BspHI TCATGA 1 cut(s) 526
BspLI GGNNCC 3 cut(s) 311, 724, 744
BspOI GCTAGC 1 cut(s) 263
BspPI GGATC 2 cut(s) 396, 817
BspQI GCTCTTC 1 cut(s) 8
BspT104I TTCGAA 1 cut(s) 218
BsrBI CCGCTC 1 cut(s) 474
BsrDI GCAATG 1 cut(s) 663
BsrI ACTGG 1 cut(s) 623
BssECI CCNNGG 2 cut(s) 313, 324
BssMI GATC 4 cut(s) 388, 487, 577, 809
BssT1I CCWWGG 2 cut(s) 313, 324
Bst2UI CCWGG 2 cut(s) 827, 927
Bst4CI ACNGT 1 cut(s) 270
Bst6I CTCTTC 1 cut(s) 8
BstBI TTCGAA 1 cut(s) 218
BstC8I GCNNGC 4 cut(s) 28, 261, 402, 693
BstDEI CTNAG 3 cut(s) 9, 252, 1003
BstDSI CCRYGG 2 cut(s) 313, 324
BstF5I GGATG 3 cut(s) 18, 154, 466
BstKTI GATC 4 cut(s) 391, 490, 580, 812
BstMAI GTCTC 3 cut(s) 372, 939, 966
BstMBI GATC 4 cut(s) 388, 487, 577, 809
BstMWI GCNNNNNNNGC 5 cut(s) 32, 429, 451, 480, 774
BstNI CCWGG 2 cut(s) 827, 927
BstNSI RCATGY 3 cut(s) 449, 513, 825
BstSCI CCNGG 2 cut(s) 825, 925
BstSFI CTRYAG 1 cut(s) 281
BstV1I GCAGC 1 cut(s) 983
BstX2I RGATCY 2 cut(s) 388, 487
BstXI CCANNNNNNTGG 1 cut(s) 868
BstYI RGATCY 2 cut(s) 388, 487
BsuI GTATCC 1 cut(s) 612
BsuRI GGCC 5 cut(s) 207, 244, 454, 471, 691
BtgI CCRYGG 2 cut(s) 313, 324
BtsCI GGATG 3 cut(s) 18, 154, 466
BtsI GCAGTG 4 cut(s) 115, 520, 784, 810
BtsIMutI CAGTG 5 cut(s) 115, 260, 520, 784, 810
Cac8I GCNNGC 4 cut(s) 28, 261, 402, 693
CciI TCATGA 1 cut(s) 526
Cfr13I GGNCC 3 cut(s) 205, 310, 689
Csp6I GTAC 3 cut(s) 278, 953, 980
CviQI GTAC 3 cut(s) 278, 953, 980
DdeI CTNAG 3 cut(s) 9, 252, 1003
DpnI GATC 4 cut(s) 390, 489, 579, 811
DpnII GATC 4 cut(s) 388, 487, 577, 809
DraI TTTAAA 1 cut(s) 658
EaeI YGGCCR 2 cut(s) 452, 469
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
Ecl136II GAGCTC 1 cut(s) 8
Eco130I CCWWGG 2 cut(s) 313, 324
Eco24I GRGCYC 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 310
Eco53kI GAGCTC 1 cut(s) 8
Eco88I CYCGRG 1 cut(s) 299
EcoICRI GAGCTC 1 cut(s) 8
EcoO109I RGGNCCY 1 cut(s) 205
EcoRII CCWGG 2 cut(s) 825, 925
EcoT14I CCWWGG 2 cut(s) 313, 324
EcoT38I GRGCYC 1 cut(s) 10
ErhI CCWWGG 2 cut(s) 313, 324
FaqI GGGAC 1 cut(s) 749
Fnu4HI GCNGC 2 cut(s) 472, 997
FokI GGATG 3 cut(s) 5, 141, 473
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 2 cut(s) 472, 997
FspBI CTAG 4 cut(s) 260, 348, 429, 993
GluI GCNGC 2 cut(s) 472, 997
HaeIII GGCC 5 cut(s) 207, 244, 454, 471, 691
HapII CCGG 1 cut(s) 451
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 6 cut(s) 139, 215, 554, 678, 932, 959
HpaI GTTAAC 1 cut(s) 49
HpaII CCGG 1 cut(s) 451
HphI GGTGA 3 cut(s) 288, 806, 941
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 1 cut(s) 12
Hpy188III TCNNGA 5 cut(s) 136, 369, 491, 527, 703
Hpy8I GTNNAC 1 cut(s) 49
HpyAV CCTTC 2 cut(s) 234, 866
HpyCH4III ACNGT 1 cut(s) 270
HpyCH4IV ACGT 1 cut(s) 51
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 429, 451, 480, 774
HpyF3I CTNAG 3 cut(s) 9, 252, 1003
HpySE526I ACGT 1 cut(s) 51
KspAI GTTAAC 1 cut(s) 49
Kzo9I GATC 4 cut(s) 388, 487, 577, 809
LguI GCTCTTC 1 cut(s) 8
LmnI GCTCC 2 cut(s) 5, 748
Lsp1109I GCAGC 1 cut(s) 983
LweI GCATC 6 cut(s) 27, 75, 156, 677, 855, 1008
MaeI CTAG 4 cut(s) 260, 348, 429, 993
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 1 cut(s) 505
MalI GATC 4 cut(s) 390, 489, 579, 811
MbiI CCGCTC 1 cut(s) 474
MboI GATC 4 cut(s) 388, 487, 577, 809
MboII GAAGA 1 cut(s) 25
MfeI CAATTG 1 cut(s) 762
MflI RGATCY 2 cut(s) 388, 487
MhlI GDGCHC 1 cut(s) 10
MluCI AATT 5 cut(s) 331, 361, 659, 762, 842
MlyI GAGTC 4 cut(s) 148, 687, 941, 968
MnlI CCTC 9 cut(s) 88, 102, 129, 261, 347, 710, 779, 790, 810
MseI TTAA 3 cut(s) 48, 648, 657
MslI CAYNNNNRTG 1 cut(s) 323
MspI CCGG 1 cut(s) 451
MspR9I CCNGG 2 cut(s) 827, 927
MunI CAATTG 1 cut(s) 762
Mva1269I GAATGC 3 cut(s) 286, 318, 695
MvaI CCWGG 2 cut(s) 827, 927
MwoI GCNNNNNNNGC 5 cut(s) 32, 429, 451, 480, 774
NcoI CCATGG 2 cut(s) 313, 324
NdeII GATC 4 cut(s) 388, 487, 577, 809
NheI GCTAGC 1 cut(s) 259
NlaIV GGNNCC 3 cut(s) 311, 724, 744
NmeAIII GCCGAG 1 cut(s) 509
NmuCI GTSAC 1 cut(s) 505
NspI RCATGY 3 cut(s) 449, 513, 825
NspV TTCGAA 1 cut(s) 218
PagI TCATGA 1 cut(s) 526
PciSI GCTCTTC 1 cut(s) 8
PctI GAATGC 3 cut(s) 286, 318, 695
PfeI GAWTC 2 cut(s) 215, 554
PkrI GCNGC 2 cut(s) 473, 998
PleI GAGTC 4 cut(s) 147, 686, 940, 967
PpsI GAGTC 4 cut(s) 147, 686, 940, 967
Psp124BI GAGCTC 1 cut(s) 10
Psp1406I AACGTT 1 cut(s) 51
Psp6I CCWGG 2 cut(s) 825, 925
PspGI CCWGG 2 cut(s) 825, 925
PspN4I GGNNCC 3 cut(s) 311, 724, 744
PspPI GGNCC 3 cut(s) 205, 310, 689
PsrI GAACNNNNNNTAC 2 cut(s) 603, 635
PsuI RGATCY 2 cut(s) 388, 487
RsaI GTAC 3 cut(s) 279, 954, 981
RsaNI GTAC 3 cut(s) 278, 953, 980
RseI CAYNNNNRTG 1 cut(s) 323
SacI GAGCTC 1 cut(s) 10
SapI GCTCTTC 1 cut(s) 8
SaqAI TTAA 3 cut(s) 48, 648, 657
SatI GCNGC 2 cut(s) 472, 997
Sau3AI GATC 4 cut(s) 388, 487, 577, 809
Sau96I GGNCC 3 cut(s) 205, 310, 689
SchI GAGTC 4 cut(s) 148, 687, 941, 968
ScrFI CCNGG 2 cut(s) 827, 927
SduI GDGCHC 1 cut(s) 10
SfaNI GCATC 6 cut(s) 27, 75, 156, 677, 855, 1008
SfcI CTRYAG 1 cut(s) 281
SfuI TTCGAA 1 cut(s) 218
SinI GGWCC 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 323
Sse9I AATT 5 cut(s) 331, 361, 659, 762, 842
SsiI CCGC 3 cut(s) 472, 720, 768
SspI AATATT 1 cut(s) 645
SspMI CTAG 4 cut(s) 260, 348, 429, 993
SstI GAGCTC 1 cut(s) 10
StyD4I CCNGG 2 cut(s) 825, 925
StyI CCWWGG 2 cut(s) 313, 324
TaaI ACNGT 1 cut(s) 270
TaiI ACGT 1 cut(s) 54
TaqI TCGA 2 cut(s) 218, 370
TaqII GACCGA 1 cut(s) 330
TasI AATT 5 cut(s) 331, 361, 659, 762, 842
TatI WGTACW 1 cut(s) 277
TauI GCSGC 1 cut(s) 474
TfiI GAWTC 2 cut(s) 215, 554
Tru1I TTAA 3 cut(s) 48, 648, 657
Tru9I TTAA 3 cut(s) 48, 648, 657
TscAI CASTG 5 cut(s) 115, 260, 520, 784, 810
TseFI GTSAC 1 cut(s) 505
TseI GCWGC 1 cut(s) 996
Tsp45I GTSAC 1 cut(s) 505
TspDTI ATGAA 3 cut(s) 652, 934, 961
TspRI CASTG 5 cut(s) 115, 260, 520, 784, 810
VpaK11BI GGWCC 1 cut(s) 310
XapI RAATTY 2 cut(s) 361, 659
XceI RCATGY 3 cut(s) 449, 513, 825
XcmI CCANNNNNNNNNTGG 1 cut(s) 252
XspI CTAG 4 cut(s) 260, 348, 429, 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.