FvH4_3g27150

F-box WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007918
Physical Location & Seq
Reverse (-)
20232892 .. 20236218
3327 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g27150.t3

Sequence Viewer

Length: 1665 bp
ATGGCATTTGAATGCCAAGTGAATACTGAGGTTTCTAAGGCTTTAATTGACTCTCTAGACAATCGACGATTAGCTGAATCTAATCACTTTCATTCCCAAATTGAAGCTAAAAATTTTGGTTTTTCAGATTCATATTCTGGAAAACAAAAAGGTGTAATTTCAAAATCCAAACCGGTTTTTAATTCCAAGAAAGCAAATCCGGACTCTTGTAGTCCGAAACAGCTTTTGTCAATCACTGACCTTCCTCCAGCTTTGATATCCGAAATTCTGAATTGCCTTGACCCGAAAGAGCTAGGCATTGTTTCATGTGCATCCACATATCTCTGTAGGCTCGCCTCTGAGCACCATGTTTGGAAGGAGTTCTATTGTGAGAGATGGGGGCTTTCAACGGCTCCAACACCCTTGGGTTCAGGACTTTCTGATGAAAAGTCATGGAAGAAGTATTTTGTCGAAAGGGAGTTTAGGAGTAAGACTTTTATGGGACGGTATAGCATGGAAATTCTGTATGGTCATACTGAACCTGTTCGAGCAGTTTTCCTTTTGTCCTCGGCAAAGCTCATATTTACATCAGGGTATGACCAAACCGTAAAAATGTGGAACATGGAAGAAGGGTTGTTGATTGCTTCTTCAAGGGGTCTTGGTTGCACTATCCGTGCAGTTGCTGCAGATACAAAGCTGTTGGTTGCTGGTGGCACTGATGGTTTTATTCACTGTTGGAAGGCAGTGGACGGCAGTCCACACTTGTTTGATATCAGGGGTCCTCAAAACCAGAACACTGAGTTTCGTCTGTGGGAGCACCAGGGTCCCATAACATCTCTTGCGTTGGATGTTTCAAGGATTTATAGTGGTTCATGGGATATGACTGTTCGTGTATGGGATCGACTTTCGCTGAAGTGTGTTAAGGTCTTGAGACATAGTGATTGGGTTGGGGAACTTGTACCTCATGATACTACAGTTGCTAGCGCAGCTGGTGCGGATGTATATGTTTGGGATATTGTTAGTGGGAATCTGATGACTATTATTCATAATGCTCATGTGGGTAAAACTTGCTCCTTGGCCAGAAACCACAAAGGGGATTTTCTTTTTACTGGAGGGGAAGATGGTGCAATTCGCATGTATGAAATTCTGAATCAGGGGCTTGAGACTAATCAACATTTGGTTGCAACATGGATCCCTCACACAGGTCCTGTGCAGTCCCTTGCGTTTGAGTTTCCATGGCTTGTTTCTGTTTCTGGTGATGGGAAGCTATCACTAATTGATGTGAGAAAGCTTTTAAAAACCAGAAGGTGTCCATCAGGAATAAGTGATTCACGTGTTAAGCATGAAGCCCGGAGTAGTGTAGAACCCCCACAGAGAATGCTACATGGATTTGCAAAGAATCTCCTCTCAGTTGACATTGGTGCTGATCGTATTGTATGTGGCGGTGAGGAAGGTGTTGTTAGGATCTGGAACTTCACACAAGCTCTGGAGATTGAGCGAAAGGCTCATGCTTTAAGAGGAATACGATTAGAGAATAGGATGAGGCGGCGTAAGCTCCATGCAGAGAATAGCAGTAAAGTTGGTCGCACTGATCAATGCTCAGTTGCAGCCAAGAAGAATCCAGTCACCGGTGAAAGGAGTGGTTGGCACAGTAAACGTGGGGCGAGTGGCAAGCTGAAAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0050896 GO:0051716 GO:0071496
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Lipase_GDSL WD40

Protein Analysis

555

Amino Acids

61.65

Weight (kDa)

9.08

Isoelectric Point (pI)

35.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 78 - 123 9.9e-07 F-box-like
Beta-prop_WDR5 PF25175 164 - 244 8e-07 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 166 - 240 1.8e-07 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 220 - 428 1.9e-11 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 223 - 419 1.5e-06 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 260 - 487 4.7e-14 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 273 - 377 6.4e-07 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 317 - 424 1.3e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 318 - 422 4.8e-09 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 199
AciI CCGC 3 cut(s) 974, 1422, 1525
AclWI GGATC 4 cut(s) 885, 1165, 1178, 1451
AcoI YGGCCR 1 cut(s) 1056
AcsI RAATTY 4 cut(s) 112, 264, 498, 1122
AcuI CTGAAG 1 cut(s) 911
AcvI CACGTG 1 cut(s) 1313
AfaI GTAC 1 cut(s) 939
AfiI CCNNNNNNNGG 4 cut(s) 1072, 1181, 1607, 1614
AflIII ACRYGT 1 cut(s) 1312
AgeI ACCGGT 2 cut(s) 172, 1607
AgsI TTSAA 6 cut(s) 11, 104, 162, 387, 630, 834
AjnI CCWGG 1 cut(s) 798
Alw21I GWGCWC 2 cut(s) 345, 798
Alw26I GTCTC 2 cut(s) 904, 1136
AlwI GGATC 4 cut(s) 885, 1165, 1178, 1451
AlwNI CAGNNNCTG 2 cut(s) 662, 1187
Aor13HI TCCGGA 1 cut(s) 199
AoxI GGCC 1 cut(s) 1056
ApeKI GCWGC 3 cut(s) 662, 965, 1586
ApoI RAATTY 4 cut(s) 112, 264, 498, 1122
AsiGI ACCGGT 2 cut(s) 172, 1607
Asp700I GAANNNNTTC 2 cut(s) 359, 522
AspLEI GCGC 1 cut(s) 965
AspS9I GGNCC 3 cut(s) 758, 803, 1184
AsuC2I CCSGG 1 cut(s) 1330
AsuHPI GGTGA 4 cut(s) 1247, 1436, 1597, 1622
AsuNHI GCTAGC 1 cut(s) 959
AvaII GGWCC 3 cut(s) 758, 803, 1184
BaeI ACNNNNGTAYC 2 cut(s) 939, 972
BalI TGGCCA 1 cut(s) 1058
BamHI GGATCC 1 cut(s) 1170
BbrPI CACGTG 1 cut(s) 1313
Bbv12I GWGCWC 2 cut(s) 345, 798
BbvI GCAGC 3 cut(s) 649, 977, 1598
BccI CCATC 5 cut(s) 369, 692, 1094, 1232, 1300
BceAI ACGGC 2 cut(s) 405, 745
BciT130I CCWGG 1 cut(s) 800
BclI TGATCA 1 cut(s) 1570
BcnI CCSGG 1 cut(s) 1330
BcoDI GTCTC 2 cut(s) 904, 1136
BfaI CTAG 4 cut(s) 56, 293, 960, 1663
BfmI CTRYAG 3 cut(s) 325, 663, 951
BisI GCNGC 4 cut(s) 663, 966, 1526, 1587
BlsI GCNGC 4 cut(s) 664, 967, 1527, 1588
Bme1390I CCNGG 2 cut(s) 800, 1330
Bme18I GGWCC 3 cut(s) 758, 803, 1184
BmgT120I GGNCC 3 cut(s) 758, 803, 1184
BmiI GGNNCC 5 cut(s) 393, 759, 804, 805, 1172
BmrFI CCNGG 2 cut(s) 800, 1330
BmsI GCATC 1 cut(s) 320
BmtI GCTAGC 1 cut(s) 963
BoxI GACNNNNGTC 1 cut(s) 732
BpmI CTGGAG 3 cut(s) 231, 1110, 1487
BpuEI CTTGAG 2 cut(s) 928, 1160
BpuMI CCSGG 1 cut(s) 1330
BsaAI YACGTR 1 cut(s) 1313
BsaJI CCNNGG 5 cut(s) 402, 546, 799, 1053, 1214
BsaWI WCCGGW 3 cut(s) 172, 199, 1607
Bsc4I CCNNNNNNNGG 4 cut(s) 1072, 1181, 1607, 1614
Bse118I RCCGGY 2 cut(s) 172, 1607
Bse1I ACTGG 2 cut(s) 1093, 1601
BseAI TCCGGA 1 cut(s) 199
BseBI CCWGG 1 cut(s) 800
BseDI CCNNGG 5 cut(s) 402, 546, 799, 1053, 1214
BseGI GGATG 4 cut(s) 311, 832, 982, 1524
BseLI CCNNNNNNNGG 4 cut(s) 1072, 1181, 1607, 1614
BseMII CTCAG 5 cut(s) 18, 330, 768, 1401, 1593
BseNI ACTGG 2 cut(s) 1093, 1601
BseRI GAGGAG 1 cut(s) 1373
BseXI GCAGC 3 cut(s) 649, 977, 1598
BsgI GTGCAG 2 cut(s) 675, 1211
BshFI GGCC 1 cut(s) 1058
BshTI ACCGGT 2 cut(s) 172, 1607
BsiHKAI GWGCWC 2 cut(s) 345, 798
BsiSI CCGG 4 cut(s) 173, 200, 1330, 1608
BslFI GGGAC 3 cut(s) 495, 789, 1180
BslI CCNNNNNNNGG 4 cut(s) 1072, 1181, 1607, 1614
BsmAI GTCTC 2 cut(s) 904, 1136
BsmFI GGGAC 3 cut(s) 495, 789, 1180
BsmI GAATGC 2 cut(s) 17, 1362
BsnI GGCC 1 cut(s) 1058
Bsp1286I GDGCHC 2 cut(s) 345, 798
Bsp13I TCCGGA 1 cut(s) 199
Bsp143I GATC 5 cut(s) 877, 1170, 1405, 1443, 1570
Bsp19I CCATGG 1 cut(s) 1214
BspACI CCGC 3 cut(s) 974, 1422, 1525
BspANI GGCC 1 cut(s) 1058
BspCNI CTCAG 5 cut(s) 19, 331, 769, 1400, 1592
BspEI TCCGGA 1 cut(s) 199
BspHI TCATGA 1 cut(s) 943
BspLI GGNNCC 5 cut(s) 393, 759, 804, 805, 1172
BspMAI CTGCAG 1 cut(s) 667
BspOI GCTAGC 1 cut(s) 963
BspPI GGATC 4 cut(s) 885, 1165, 1178, 1451
BsrFI RCCGGY 2 cut(s) 172, 1607
BsrI ACTGG 2 cut(s) 1093, 1601
BssAI RCCGGY 2 cut(s) 172, 1607
BssECI CCNNGG 5 cut(s) 402, 546, 799, 1053, 1214
BssMI GATC 5 cut(s) 877, 1170, 1405, 1443, 1570
BssT1I CCWWGG 3 cut(s) 402, 1053, 1214
Bst2UI CCWGG 1 cut(s) 800
Bst4CI ACNGT 6 cut(s) 486, 586, 713, 865, 955, 1631
BstAPI GCANNNNNTGC 2 cut(s) 662, 971
BstBAI YACGTR 1 cut(s) 1313
BstC8I GCNNGC 3 cut(s) 333, 961, 1652
BstDEI CTNAG 6 cut(s) 27, 36, 339, 777, 1387, 1579
BstDSI CCRYGG 1 cut(s) 1214
BstENI CCTNNNNNAGG 1 cut(s) 1179
BstF5I GGATG 4 cut(s) 311, 832, 982, 1524
BstHHI GCGC 1 cut(s) 965
BstKTI GATC 5 cut(s) 880, 1173, 1408, 1446, 1573
BstMAI GTCTC 2 cut(s) 904, 1136
BstMBI GATC 5 cut(s) 877, 1170, 1405, 1443, 1570
BstMWI GCNNNNNNNGC 4 cut(s) 662, 965, 971, 1531
BstNI CCWGG 1 cut(s) 800
BstNSI RCATGY 1 cut(s) 1117
BstPAI GACNNNNGTC 1 cut(s) 732
BstSCI CCNGG 2 cut(s) 798, 1328
BstSFI CTRYAG 3 cut(s) 325, 663, 951
BstV1I GCAGC 3 cut(s) 649, 977, 1598
BstX2I RGATCY 2 cut(s) 1170, 1443
BstYI RGATCY 2 cut(s) 1170, 1443
BsuRI GGCC 1 cut(s) 1058
BtgI CCRYGG 1 cut(s) 1214
BtsCI GGATG 4 cut(s) 311, 832, 982, 1524
BtsI GCAGTG 1 cut(s) 729
BtsIMutI CAGTG 6 cut(s) 234, 693, 709, 729, 774, 1566
Cac8I GCNNGC 3 cut(s) 333, 961, 1652
CaiI CAGNNNCTG 2 cut(s) 662, 1187
CciI TCATGA 1 cut(s) 943
CfoI GCGC 1 cut(s) 965
Cfr10I RCCGGY 2 cut(s) 172, 1607
Cfr13I GGNCC 3 cut(s) 758, 803, 1184
Csp6I GTAC 1 cut(s) 938
CspAI ACCGGT 2 cut(s) 172, 1607
CviQI GTAC 1 cut(s) 938
DdeI CTNAG 6 cut(s) 27, 36, 339, 777, 1387, 1579
DpnI GATC 5 cut(s) 879, 1172, 1407, 1445, 1572
DpnII GATC 5 cut(s) 877, 1170, 1405, 1443, 1570
DraI TTTAAA 1 cut(s) 1275
EaeI YGGCCR 1 cut(s) 1056
Eco130I CCWWGG 3 cut(s) 402, 1053, 1214
Eco32I GATATC 2 cut(s) 258, 751
Eco47I GGWCC 3 cut(s) 758, 803, 1184
Eco57I CTGAAG 1 cut(s) 911
Eco72I CACGTG 1 cut(s) 1313
EcoNI CCTNNNNNAGG 1 cut(s) 1179
EcoO109I RGGNCCY 3 cut(s) 758, 803, 1184
EcoRII CCWGG 1 cut(s) 798
EcoRV GATATC 2 cut(s) 258, 751
EcoT14I CCWWGG 3 cut(s) 402, 1053, 1214
ErhI CCWWGG 3 cut(s) 402, 1053, 1214
FaqI GGGAC 3 cut(s) 495, 789, 1180
FbaI TGATCA 1 cut(s) 1570
Fnu4HI GCNGC 4 cut(s) 663, 966, 1526, 1587
FokI GGATG 4 cut(s) 298, 839, 989, 1531
Fsp4HI GCNGC 4 cut(s) 663, 966, 1526, 1587
FspBI CTAG 4 cut(s) 56, 293, 960, 1663
GlaI GCGC 1 cut(s) 964
GluI GCNGC 4 cut(s) 663, 966, 1526, 1587
GsuI CTGGAG 3 cut(s) 231, 1110, 1487
HaeIII GGCC 1 cut(s) 1058
HapII CCGG 4 cut(s) 173, 200, 1330, 1608
HhaI GCGC 1 cut(s) 965
Hin6I GCGC 1 cut(s) 963
HinP1I GCGC 1 cut(s) 963
HincII GTYRAC 1 cut(s) 1393
HindII GTYRAC 1 cut(s) 1393
HindIII AAGCTT 1 cut(s) 1268
HinfI GANTC 9 cut(s) 50, 77, 128, 203, 1006, 1129, 1307, 1378, 1597
HpaII CCGG 4 cut(s) 173, 200, 1330, 1608
HphI GGTGA 4 cut(s) 1247, 1436, 1597, 1622
Hpy166II GTNNAC 4 cut(s) 727, 737, 1393, 1634
Hpy188I TCNGA 8 cut(s) 127, 216, 262, 270, 340, 421, 1011, 1128
Hpy188III TCNNGA 9 cut(s) 56, 138, 200, 411, 907, 944, 1296, 1447, 1466
Hpy8I GTNNAC 4 cut(s) 727, 737, 1393, 1634
Hpy99I CGWCG 1 cut(s) 69
HpyAV CCTTC 6 cut(s) 251, 349, 602, 712, 1278, 1424
HpyCH4III ACNGT 6 cut(s) 486, 586, 713, 865, 955, 1631
HpyCH4IV ACGT 2 cut(s) 1312, 1636
HpyF10VI GCNNNNNNNGC 4 cut(s) 662, 965, 971, 1531
HpyF3I CTNAG 6 cut(s) 27, 36, 339, 777, 1387, 1579
HpySE526I ACGT 2 cut(s) 1312, 1636
HspAI GCGC 1 cut(s) 963
KflI GGGWCCC 1 cut(s) 803
Kpn2I TCCGGA 1 cut(s) 199
Ksp22I TGATCA 1 cut(s) 1570
Kzo9I GATC 5 cut(s) 877, 1170, 1405, 1443, 1570
LmnI GCTCC 4 cut(s) 397, 793, 1055, 1539
Lsp1109I GCAGC 3 cut(s) 649, 977, 1598
LweI GCATC 1 cut(s) 320
MaeI CTAG 4 cut(s) 56, 293, 960, 1663
MaeII ACGT 2 cut(s) 1312, 1636
MaeIII GTNAC 1 cut(s) 1603
MalI GATC 5 cut(s) 879, 1172, 1407, 1445, 1572
MboI GATC 5 cut(s) 877, 1170, 1405, 1443, 1570
MboII GAAGA 5 cut(s) 448, 617, 618, 1109, 1606
MflI RGATCY 2 cut(s) 1170, 1443
MhlI GDGCHC 2 cut(s) 345, 798
MlsI TGGCCA 1 cut(s) 1058
MluNI TGGCCA 1 cut(s) 1058
MlyI GAGTC 2 cut(s) 44, 197
MmeI TCCRAC 3 cut(s) 419, 695, 804
Mox20I TGGCCA 1 cut(s) 1058
MroI TCCGGA 1 cut(s) 199
MroXI GAANNNNTTC 2 cut(s) 359, 522
MscI TGGCCA 1 cut(s) 1058
MseI TTAA 6 cut(s) 44, 180, 900, 1274, 1317, 1493
MslI CAYNNNNRTG 1 cut(s) 10
Msp20I TGGCCA 1 cut(s) 1058
MspA1I CMGCKG 1 cut(s) 968
MspI CCGG 4 cut(s) 173, 200, 1330, 1608
MspR9I CCNGG 2 cut(s) 800, 1330
Mva1269I GAATGC 2 cut(s) 17, 1362
MvaI CCWGG 1 cut(s) 800
MwoI GCNNNNNNNGC 4 cut(s) 662, 965, 971, 1531
NciI CCSGG 1 cut(s) 1330
NcoI CCATGG 1 cut(s) 1214
NdeII GATC 5 cut(s) 877, 1170, 1405, 1443, 1570
NheI GCTAGC 1 cut(s) 959
NlaIV GGNNCC 5 cut(s) 393, 759, 804, 805, 1172
NmeAIII GCCGAG 1 cut(s) 527
NmuCI GTSAC 1 cut(s) 1603
NspI RCATGY 1 cut(s) 1117
PagI TCATGA 1 cut(s) 943
PctI GAATGC 2 cut(s) 17, 1362
PdmI GAANNNNTTC 2 cut(s) 359, 522
PfeI GAWTC 7 cut(s) 77, 128, 1006, 1129, 1307, 1378, 1597
PinAI ACCGGT 2 cut(s) 172, 1607
PkrI GCNGC 4 cut(s) 664, 967, 1527, 1588
PleI GAGTC 2 cut(s) 44, 197
PmaCI CACGTG 1 cut(s) 1313
PmlI CACGTG 1 cut(s) 1313
PpsI GAGTC 2 cut(s) 44, 197
Ppu21I YACGTR 1 cut(s) 1313
PpuMI RGGWCCY 3 cut(s) 758, 803, 1184
PshAI GACNNNNGTC 1 cut(s) 732
Psp5II RGGWCCY 3 cut(s) 758, 803, 1184
Psp6I CCWGG 1 cut(s) 798
PspCI CACGTG 1 cut(s) 1313
PspGI CCWGG 1 cut(s) 798
PspN4I GGNNCC 5 cut(s) 393, 759, 804, 805, 1172
PspPI GGNCC 3 cut(s) 758, 803, 1184
PspPPI RGGWCCY 3 cut(s) 758, 803, 1184
PstI CTGCAG 1 cut(s) 667
PstNI CAGNNNCTG 2 cut(s) 662, 1187
PsuI RGATCY 2 cut(s) 1170, 1443
PvuII CAGCTG 1 cut(s) 968
RsaI GTAC 1 cut(s) 939
RsaNI GTAC 1 cut(s) 938
RseI CAYNNNNRTG 1 cut(s) 10
SaqAI TTAA 6 cut(s) 44, 180, 900, 1274, 1317, 1493
SatI GCNGC 4 cut(s) 663, 966, 1526, 1587
Sau3AI GATC 5 cut(s) 877, 1170, 1405, 1443, 1570
Sau96I GGNCC 3 cut(s) 758, 803, 1184
SchI GAGTC 2 cut(s) 44, 197
ScrFI CCNGG 2 cut(s) 800, 1330
SduI GDGCHC 2 cut(s) 345, 798
SfaNI GCATC 1 cut(s) 320
SfcI CTRYAG 3 cut(s) 325, 663, 951
SgrAI CRCCGGYG 1 cut(s) 1607
SinI GGWCC 3 cut(s) 758, 803, 1184
SmiMI CAYNNNNRTG 1 cut(s) 10
SmlI CTYRAG 2 cut(s) 907, 1139
SmoI CTYRAG 2 cut(s) 907, 1139
SsiI CCGC 3 cut(s) 974, 1422, 1525
SspMI CTAG 4 cut(s) 56, 293, 960, 1663
StyD4I CCNGG 2 cut(s) 798, 1328
StyI CCWWGG 3 cut(s) 402, 1053, 1214
TaaI ACNGT 6 cut(s) 486, 586, 713, 865, 955, 1631
TaiI ACGT 2 cut(s) 1315, 1639
TaqI TCGA 4 cut(s) 64, 450, 526, 880
TauI GCSGC 1 cut(s) 1528
TfiI GAWTC 7 cut(s) 77, 128, 1006, 1129, 1307, 1378, 1597
Tru1I TTAA 6 cut(s) 44, 180, 900, 1274, 1317, 1493
Tru9I TTAA 6 cut(s) 44, 180, 900, 1274, 1317, 1493
TscAI CASTG 6 cut(s) 241, 700, 716, 729, 781, 1573
TseFI GTSAC 1 cut(s) 1603
TseI GCWGC 3 cut(s) 662, 965, 1586
Tsp45I GTSAC 1 cut(s) 1603
TspDTI ATGAA 8 cut(s) 80, 120, 294, 438, 840, 1013, 1134, 1338
TspGWI ACGGA 1 cut(s) 641
TspRI CASTG 6 cut(s) 241, 700, 716, 729, 781, 1573
VpaK11BI GGWCC 3 cut(s) 758, 803, 1184
XagI CCTNNNNNAGG 1 cut(s) 1179
XapI RAATTY 4 cut(s) 112, 264, 498, 1122
XbaI TCTAGA 1 cut(s) 55
XceI RCATGY 1 cut(s) 1117
XmnI GAANNNNTTC 2 cut(s) 359, 522
XspI CTAG 4 cut(s) 56, 293, 960, 1663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.