RLG00000034647

F-box WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0007918
Physical Location & Seq
Reverse (-)
47031086 .. 47033054
1969 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034647

Sequence Viewer

Length: 1281 bp
ATGGCATTTGAATGCCAAGTGAATACTGAGGTTTCTAAGAATTTGATCGACTCTCTAGACAATCCGCTGGGACAATTAGCTGAATCTAATCAATTTCATTCTCAAATTGAAGCTGAAAATTTTGGTGTTTCAAAAGTAGCTTCAGATTCATATACTGAAAAAGAAAAACGTGTAATTTCAAAACCCAAACCAGTTTCTAGTTCCAAGAAAGCAAATCCGGACTCTTGTGGTTCGAAACAGCTTTTGTCAACTGATCGGTCAATCACTGACCTTCCTCCAGCTTTGATATCTGAAATTCTGAATTGCCTTGACCCGAAAGAGCTAGGCATAGTTTCATGTGCATCGACATATCTCTGTAGGCTCGCATCTGAGCACCATGTTTGGAAGGAGTTTTATTGTGAGAGATGGGGACTTTCAGCGGCTCCAGCATCCTTGGGTTCAGGACTTTCTGATGACAAGTCATGGAAGGAGTTTTTTGTCGAAAGGGAGTTTAGGAGCAAGACTTTTATGGGACGGGGTCCTCAAAATGAGAATACTGAGTTTCGTCTGTGGGAGCATCAGGGTCCTATAACATCTCTTGCTTTGGATTTTACAAGGATTTATAGTGGTTCGTGGGATATGACTGTTCGTGTATGGGATCGACTTTCACTGGAGTGTGTTAAGGTCCTGCGCCATAGTGATTGGGTTGGGGGACTTGCACCTCATGATACTACAGTTGCTAGCACAGCTGGTTCGGATGTCTATGTTTGGGACACTAGCAGTGGGAATCTGATGACCATTATTCATAATGCTCATGTTGGCAAAACTTGCTCCTTGGCCAGAAACCACAAAGGGGATTTTCTATTTACCGGAGGGGAAGATGGTGCAATTCACATGTATGAAATTCTGAGTCATGGGCTTGAGACTAATGAATATTTGGTTGCAACATGGATCCCTCACTCAGGCCCTTTGCATTCCCTTGCGTTTGAGTTTCCATGGCTTGTTTCTGCTTCTGGTGATGGGAAGCTATCACTAATTGATGTGAGAAAGCTTTTAAAAACTAGAAAGCGTGCTTCAGGGACAAGTGCTTCATGTGTTAAGCATGGAGACTGGAGTAGTGTAGAACCCCCACAGAGAATGCTACATGGATTTGCAAAGAATCTCCTCTCAGTTGACATTGGTGCTGATCGTATTGATGATTATGTGATTTTGTACAACTTATGGCTTAAGCAGACGAAAGAATTGAAAGAATTTTTGTTTCTTGCTTTTCAAAGATCTCTTATTGTTAGAGAATTATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0050896 GO:0051716 GO:0071496
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Lipase_GDSL WD40

Protein Analysis

427

Amino Acids

47.7

Weight (kDa)

6.12

Isoelectric Point (pI)

31.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 88 - 133 7e-07 F-box-like
Beta-prop_THOC3 PF25174 176 - 348 7.4e-10 THOC3 beta-propeller domain
WD40_Prp19 PF24814 179 - 351 6.8e-10 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 193 - 296 2e-06 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 196 - 297 1.2e-10 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 238 - 342 8.9e-08 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 238 - 344 3.2e-06 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 2 cut(s) 65, 419
AclWI GGATC 3 cut(s) 645, 925, 938
AcoI YGGCCR 1 cut(s) 816
AcsI RAATTY 5 cut(s) 40, 118, 294, 882, 1229
AcuI CTGAAG 2 cut(s) 126, 1038
AfaI GTAC 1 cut(s) 1193
AfiI CCNNNNNNNGG 2 cut(s) 832, 941
AflII CTTAAG 1 cut(s) 1205
AflIII ACRYGT 2 cut(s) 169, 873
AgsI TTSAA 6 cut(s) 11, 110, 132, 180, 1225, 1250
AleI CACNNNNGTG 1 cut(s) 652
AluBI AGCT 9 cut(s) 80, 113, 140, 241, 281, 322, 728, 1006, 1030
AluI AGCT 9 cut(s) 80, 113, 140, 241, 281, 322, 728, 1006, 1030
Alw21I GWGCWC 1 cut(s) 375
Alw26I GTCTC 2 cut(s) 896, 1080
AlwI GGATC 3 cut(s) 645, 925, 938
Aor13HI TCCGGA 1 cut(s) 217
AoxI GGCC 2 cut(s) 816, 943
ApoI RAATTY 5 cut(s) 40, 118, 294, 882, 1229
AspLEI GCGC 1 cut(s) 672
AspS9I GGNCC 4 cut(s) 518, 563, 664, 944
AsuHPI GGTGA 1 cut(s) 1007
AsuII TTCGAA 1 cut(s) 233
AsuNHI GCTAGC 1 cut(s) 719
AvaII GGWCC 3 cut(s) 518, 563, 664
BaeI ACNNNNGTAYC 2 cut(s) 699, 732
BalI TGGCCA 1 cut(s) 818
BamHI GGATCC 1 cut(s) 930
Bbv12I GWGCWC 1 cut(s) 375
BccI CCATC 3 cut(s) 399, 854, 992
BcoDI GTCTC 2 cut(s) 896, 1080
BfaI CTAG 6 cut(s) 56, 198, 323, 720, 756, 1041
BfmI CTRYAG 2 cut(s) 355, 711
BfrI CTTAAG 1 cut(s) 1205
BglII AGATCT 1 cut(s) 1253
BisI GCNGC 1 cut(s) 420
BlsI GCNGC 1 cut(s) 421
Bme18I GGWCC 3 cut(s) 518, 563, 664
BmgT120I GGNCC 4 cut(s) 518, 563, 664, 944
BmiI GGNNCC 4 cut(s) 423, 519, 564, 932
BmsI GCATC 4 cut(s) 350, 374, 437, 565
BmtI GCTAGC 1 cut(s) 723
BpmI CTGGAG 4 cut(s) 261, 408, 671, 1111
Bpu14I TTCGAA 1 cut(s) 233
BpuEI CTTGAG 1 cut(s) 920
BsaJI CCNNGG 3 cut(s) 432, 813, 974
BsaWI WCCGGW 2 cut(s) 217, 848
Bsc4I CCNNNNNNNGG 2 cut(s) 832, 941
Bse1I ACTGG 3 cut(s) 191, 654, 1094
BseAI TCCGGA 1 cut(s) 217
BseDI CCNNGG 3 cut(s) 432, 813, 974
BseGI GGATG 2 cut(s) 428, 742
BseLI CCNNNNNNNGG 2 cut(s) 832, 941
BseMII CTCAG 6 cut(s) 18, 360, 528, 878, 954, 1161
BseNI ACTGG 3 cut(s) 191, 654, 1094
BseRI GAGGAG 1 cut(s) 1133
BseYI CCCAGC 1 cut(s) 67
BshFI GGCC 2 cut(s) 818, 945
BsiHKAI GWGCWC 1 cut(s) 375
BsiSI CCGG 2 cut(s) 218, 849
BslFI GGGAC 6 cut(s) 84, 423, 525, 705, 764, 1072
BslI CCNNNNNNNGG 2 cut(s) 832, 941
BsmAI GTCTC 2 cut(s) 896, 1080
BsmFI GGGAC 6 cut(s) 84, 423, 525, 705, 764, 1072
BsmI GAATGC 3 cut(s) 17, 952, 1122
BsnI GGCC 2 cut(s) 818, 945
Bsp119I TTCGAA 1 cut(s) 233
Bsp1286I GDGCHC 1 cut(s) 375
Bsp13I TCCGGA 1 cut(s) 217
Bsp1407I TGTACA 1 cut(s) 1191
Bsp143I GATC 6 cut(s) 45, 253, 637, 930, 1165, 1253
Bsp19I CCATGG 1 cut(s) 974
BspACI CCGC 2 cut(s) 65, 419
BspANI GGCC 2 cut(s) 818, 945
BspCNI CTCAG 6 cut(s) 19, 361, 529, 879, 953, 1160
BspEI TCCGGA 1 cut(s) 217
BspHI TCATGA 1 cut(s) 703
BspLI GGNNCC 4 cut(s) 423, 519, 564, 932
BspOI GCTAGC 1 cut(s) 723
BspPI GGATC 3 cut(s) 645, 925, 938
BspT104I TTCGAA 1 cut(s) 233
BspTI CTTAAG 1 cut(s) 1205
BsrGI TGTACA 1 cut(s) 1191
BsrI ACTGG 3 cut(s) 191, 654, 1094
BssECI CCNNGG 3 cut(s) 432, 813, 974
BssMI GATC 6 cut(s) 45, 253, 637, 930, 1165, 1253
BssT1I CCWWGG 3 cut(s) 432, 813, 974
Bst4CI ACNGT 2 cut(s) 625, 715
BstAFI CTTAAG 1 cut(s) 1205
BstAPI GCANNNNNTGC 1 cut(s) 807
BstAUI TGTACA 1 cut(s) 1191
BstBI TTCGAA 1 cut(s) 233
BstC8I GCNNGC 3 cut(s) 363, 721, 1050
BstDEI CTNAG 7 cut(s) 27, 36, 369, 537, 887, 940, 1147
BstDSI CCRYGG 1 cut(s) 974
BstENI CCTNNNNNAGG 1 cut(s) 939
BstF5I GGATG 2 cut(s) 428, 742
BstHHI GCGC 1 cut(s) 672
BstKTI GATC 6 cut(s) 48, 256, 640, 933, 1168, 1256
BstMAI GTCTC 2 cut(s) 896, 1080
BstMBI GATC 6 cut(s) 45, 253, 637, 930, 1165, 1253
BstMWI GCNNNNNNNGC 3 cut(s) 425, 725, 807
BstNSI RCATGY 1 cut(s) 877
BstSFI CTRYAG 2 cut(s) 355, 711
BstX2I RGATCY 2 cut(s) 930, 1253
BstYI RGATCY 2 cut(s) 930, 1253
BsuRI GGCC 2 cut(s) 818, 945
BtgI CCRYGG 1 cut(s) 974
BtsCI GGATG 2 cut(s) 428, 742
BtsI GCAGTG 1 cut(s) 766
BtsIMutI CAGTG 3 cut(s) 264, 647, 766
Cac8I GCNNGC 3 cut(s) 363, 721, 1050
CciI TCATGA 1 cut(s) 703
CfoI GCGC 1 cut(s) 672
Cfr13I GGNCC 4 cut(s) 518, 563, 664, 944
Csp6I GTAC 1 cut(s) 1192
CviQI GTAC 1 cut(s) 1192
DdeI CTNAG 7 cut(s) 27, 36, 369, 537, 887, 940, 1147
DpnI GATC 6 cut(s) 47, 255, 639, 932, 1167, 1255
DpnII GATC 6 cut(s) 45, 253, 637, 930, 1165, 1253
DraI TTTAAA 1 cut(s) 1035
EaeI YGGCCR 1 cut(s) 816
Eco130I CCWWGG 3 cut(s) 432, 813, 974
Eco32I GATATC 1 cut(s) 288
Eco47I GGWCC 3 cut(s) 518, 563, 664
Eco57I CTGAAG 2 cut(s) 126, 1038
EcoNI CCTNNNNNAGG 1 cut(s) 939
EcoO109I RGGNCCY 4 cut(s) 518, 563, 664, 944
EcoRV GATATC 1 cut(s) 288
EcoT14I CCWWGG 3 cut(s) 432, 813, 974
ErhI CCWWGG 3 cut(s) 432, 813, 974
FaqI GGGAC 6 cut(s) 84, 423, 525, 705, 764, 1072
Fnu4HI GCNGC 1 cut(s) 420
FokI GGATG 2 cut(s) 415, 749
Fsp4HI GCNGC 1 cut(s) 420
FspBI CTAG 6 cut(s) 56, 198, 323, 720, 756, 1041
GlaI GCGC 1 cut(s) 671
GluI GCNGC 1 cut(s) 420
GsaI CCCAGC 1 cut(s) 71
GsuI CTGGAG 4 cut(s) 261, 408, 671, 1111
HaeIII GGCC 2 cut(s) 818, 945
HapII CCGG 2 cut(s) 218, 849
HhaI GCGC 1 cut(s) 672
Hin6I GCGC 1 cut(s) 670
HinP1I GCGC 1 cut(s) 670
HincII GTYRAC 2 cut(s) 249, 1153
HindII GTYRAC 2 cut(s) 249, 1153
HindIII AAGCTT 1 cut(s) 1028
HinfI GANTC 7 cut(s) 50, 83, 146, 221, 766, 889, 1138
HpaII CCGG 2 cut(s) 218, 849
HphI GGTGA 1 cut(s) 1007
Hpy166II GTNNAC 2 cut(s) 249, 1153
Hpy188I TCNGA 8 cut(s) 145, 292, 300, 370, 451, 736, 771, 888
Hpy188III TCNNGA 4 cut(s) 56, 218, 441, 704
Hpy8I GTNNAC 2 cut(s) 249, 1153
HpyAV CCTTC 3 cut(s) 281, 379, 460
HpyCH4III ACNGT 2 cut(s) 625, 715
HpyCH4IV ACGT 1 cut(s) 169
HpyCH4V TGCA 6 cut(s) 341, 698, 866, 923, 952, 1133
HpyF10VI GCNNNNNNNGC 3 cut(s) 425, 725, 807
HpyF3I CTNAG 7 cut(s) 27, 36, 369, 537, 887, 940, 1147
HpySE526I ACGT 1 cut(s) 169
HspAI GCGC 1 cut(s) 670
Kpn2I TCCGGA 1 cut(s) 217
Kzo9I GATC 6 cut(s) 45, 253, 637, 930, 1165, 1253
LmnI GCTCC 4 cut(s) 427, 495, 553, 815
LweI GCATC 4 cut(s) 350, 374, 437, 565
MaeI CTAG 6 cut(s) 56, 198, 323, 720, 756, 1041
MaeII ACGT 1 cut(s) 169
MalI GATC 6 cut(s) 47, 255, 639, 932, 1167, 1255
MboI GATC 6 cut(s) 45, 253, 637, 930, 1165, 1253
MboII GAAGA 1 cut(s) 869
MflI RGATCY 2 cut(s) 930, 1253
MhlI GDGCHC 1 cut(s) 375
MlsI TGGCCA 1 cut(s) 818
MluNI TGGCCA 1 cut(s) 818
MlyI GAGTC 3 cut(s) 44, 215, 898
MnlI CCTC 7 cut(s) 22, 285, 531, 711, 845, 945, 1154
Mox20I TGGCCA 1 cut(s) 818
MroI TCCGGA 1 cut(s) 217
MscI TGGCCA 1 cut(s) 818
MseI TTAA 4 cut(s) 660, 1034, 1077, 1206
MslI CAYNNNNRTG 3 cut(s) 10, 652, 876
Msp20I TGGCCA 1 cut(s) 818
MspA1I CMGCKG 3 cut(s) 67, 419, 728
MspCI CTTAAG 1 cut(s) 1205
MspI CCGG 2 cut(s) 218, 849
Mva1269I GAATGC 3 cut(s) 17, 952, 1122
MwoI GCNNNNNNNGC 3 cut(s) 425, 725, 807
NcoI CCATGG 1 cut(s) 974
NdeII GATC 6 cut(s) 45, 253, 637, 930, 1165, 1253
NheI GCTAGC 1 cut(s) 719
NlaIV GGNNCC 4 cut(s) 423, 519, 564, 932
NspI RCATGY 1 cut(s) 877
NspV TTCGAA 1 cut(s) 233
OliI CACNNNNGTG 1 cut(s) 652
PagI TCATGA 1 cut(s) 703
PciI ACATGT 1 cut(s) 873
PctI GAATGC 3 cut(s) 17, 952, 1122
PfeI GAWTC 4 cut(s) 83, 146, 766, 1138
PflFI GACNNNGTC 1 cut(s) 516
PkrI GCNGC 1 cut(s) 421
PleI GAGTC 3 cut(s) 44, 215, 897
PpsI GAGTC 3 cut(s) 44, 215, 897
PpuMI RGGWCCY 3 cut(s) 518, 563, 664
PscI ACATGT 1 cut(s) 873
Psp5II RGGWCCY 3 cut(s) 518, 563, 664
PspFI CCCAGC 1 cut(s) 67
PspN4I GGNNCC 4 cut(s) 423, 519, 564, 932
PspPI GGNCC 4 cut(s) 518, 563, 664, 944
PspPPI RGGWCCY 3 cut(s) 518, 563, 664
PsuI RGATCY 2 cut(s) 930, 1253
PsyI GACNNNGTC 1 cut(s) 516
PvuII CAGCTG 1 cut(s) 728
RsaI GTAC 1 cut(s) 1193
RsaNI GTAC 1 cut(s) 1192
RseI CAYNNNNRTG 3 cut(s) 10, 652, 876
SaqAI TTAA 4 cut(s) 660, 1034, 1077, 1206
SatI GCNGC 1 cut(s) 420
Sau3AI GATC 6 cut(s) 45, 253, 637, 930, 1165, 1253
Sau96I GGNCC 4 cut(s) 518, 563, 664, 944
SchI GAGTC 3 cut(s) 44, 215, 898
SduI GDGCHC 1 cut(s) 375
SfaNI GCATC 4 cut(s) 350, 374, 437, 565
SfcI CTRYAG 2 cut(s) 355, 711
SfuI TTCGAA 1 cut(s) 233
SinI GGWCC 3 cut(s) 518, 563, 664
SmiMI CAYNNNNRTG 3 cut(s) 10, 652, 876
SmlI CTYRAG 2 cut(s) 899, 1205
SmoI CTYRAG 2 cut(s) 899, 1205
SsiI CCGC 2 cut(s) 65, 419
SspI AATATT 1 cut(s) 914
SspMI CTAG 6 cut(s) 56, 198, 323, 720, 756, 1041
StyI CCWWGG 3 cut(s) 432, 813, 974
TaaI ACNGT 2 cut(s) 625, 715
TaiI ACGT 1 cut(s) 172
TaqI TCGA 5 cut(s) 48, 233, 344, 480, 640
TaqII GACCGA 1 cut(s) 246
TatI WGTACW 1 cut(s) 1191
TauI GCSGC 1 cut(s) 422
TfiI GAWTC 4 cut(s) 83, 146, 766, 1138
Tru1I TTAA 4 cut(s) 660, 1034, 1077, 1206
Tru9I TTAA 4 cut(s) 660, 1034, 1077, 1206
TscAI CASTG 3 cut(s) 271, 654, 766
TspDTI ATGAA 7 cut(s) 86, 138, 324, 773, 894, 924, 1059
TspRI CASTG 3 cut(s) 271, 654, 766
Tth111I GACNNNGTC 1 cut(s) 516
Vha464I CTTAAG 1 cut(s) 1205
VpaK11BI GGWCC 3 cut(s) 518, 563, 664
XagI CCTNNNNNAGG 1 cut(s) 939
XapI RAATTY 5 cut(s) 40, 118, 294, 882, 1229
XbaI TCTAGA 1 cut(s) 55
XceI RCATGY 1 cut(s) 877
XspI CTAG 6 cut(s) 56, 198, 323, 720, 756, 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.