Rw5G030910

F-box WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007918
Physical Location & Seq
Reverse (-)
49513560 .. 49515253
1694 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G030910.1

Sequence Viewer

Length: 1620 bp
ATGGCATTTGAATGCCAAGTGAATACTGAGGTTTCTAAGAATTTCATCGACTCTCTAGACAATCCGCTCGGACAATTAGCTGAATCTAACCAATTTCATTCTCAAATTGAAGCTGAAAGTTTTGGTGTTTCAAAAGTAGCTTCAGATTCATATACTAAAAAAGAAAAAGGTGTAATTTCAAAACCCAAACCAGTTTCTAGTTCCAAGAAAGCAAATCCGGACTCTTGTGGTTCGAAACAGCTTTTGTCAACTGATCGGTCAATCACTGACCTTCCTCCAGCTTTGATATCCGAAATTCTGAATTGCCTTGACCCGAAAGAGCTAGGCATAGTTTCATGTGCATCGACATATCTCTGTAGGCTCGCATCTGAGCACCATGTTTGGAAGGAGTTTTATTGTGAGAGATGGGGACTTTCAGCGGCTCCAGCATCCTTGGGTTCAGGACTTTCTGATGACAAGTCATGGAAGGAGTTTTTTGTCGAAAGGGAGTTTAGGAGCAAGACTTTTATGGGACGGTATAGCATGGAAATTTTGTATGGTCATACTGAACCTGTTCGAACAGTTTTCCTTTTGGCCTCGGCAAAGATCATATTTACGTCAGGTTATGACCAAACCGTAAAAATGTGGAACATGGAAGAAGGGCTGTTGATTGCTTCTTCAAGGTCTCTTGGTTGCACTATCCGTGCAGTTGCTGCAGATACAAAACTCTTGGGGTCCTCAAAATGGAATACTGAGTTTCGTCTGTGGGAGCATCAGGGTCCTATAACATCTCTTGCGTTGGATTTTACAAGGATTTACAGTGGTTCGTGGGATATGACTGTTCGTGTATGGGATCGACTTTCACTGAAGTGTGTTAAGGTCCTGCGCCATAGTGATTGGGTCGGGGGAATTGCACCTCATGATACTACAGTTGCTAGCACAGCTGGTTCGGATGTCTATGTTTGGGACACTAGCAGTGGGAATCTGATGACCATTATTCATAATGCTCATGTTGGCAAAACTTGCTCCTTGGCCAGAAACCACAAAGGGGATTTTCTATTTACCGGAGGGGAAGATGGTGCAATTCACATGTATGAAATTCTGAGTCATGGGCTTGAGACTAATGAATATTTGGTTGCAACATGGATCCCTCACTCAGGCCCTGTGCATTCCCTTGCGTTTGAGTTTCCATGGCTTGTTTCTGCTTCTGGTGATGGGAAGCTATCACTAATTGATGTGAGAAAGCTTTTAAAAACTAGAAAGCGTGCTTCAGGGACAAGTGCTTCATGTGTTAAGCTTGGAGACCAGAGTAGTGTAGAACCCCCACAGAGAATGCTACATGGATTTGCAAAGAATCTCCTCTCAGTCGACATTGGTGCTGATCGTATTGTATGTGGGGGTGAGGAAGGTGTTGTTAGGATCTGGAACTTCACACAAGCTCTGGAGATTGAGCGAAGGGCTAATGCTTTAAGAGGAATACGATTAGAGAATAGGATGAGGCGGCGTAAGCTCCATGTAGAGAATAGCAGCAAAGGTGGTCGCACTGATCAATGCTCAGTTGCAGCCAAGAAGAATCCAGTCACTGGTGAAAGGAGTGGTTGGCACAGTAAACGTGGGGCGAGTGGCAAGCTGAGAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0050896 GO:0051716 GO:0071496
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Lipase_GDSL WD40

Protein Analysis

539

Amino Acids

59.6

Weight (kDa)

8.85

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 88 - 133 9.6e-07 F-box-like
WD40_Prp19 PF24814 248 - 417 4e-10 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 253 - 388 3.2e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 258 - 361 2.5e-06 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 261 - 424 7.2e-10 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 263 - 472 2.7e-13 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 303 - 407 1.6e-08 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 303 - 409 7.4e-07 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1562
AccBSI CCGCTC 1 cut(s) 67
AccI GTMKAC 1 cut(s) 1347
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 3 cut(s) 65, 419, 1480
AclWI GGATC 4 cut(s) 840, 1120, 1133, 1406
AcoI YGGCCR 1 cut(s) 1011
AcsI RAATTY 4 cut(s) 40, 294, 528, 1077
AcuI CTGAAG 3 cut(s) 126, 866, 1233
AfiI CCNNNNNNNGG 4 cut(s) 723, 1027, 1136, 1562
AflIII ACRYGT 1 cut(s) 1068
AgsI TTSAA 5 cut(s) 11, 110, 132, 180, 660
AleI CACNNNNGTG 1 cut(s) 847
Alw21I GWGCWC 1 cut(s) 375
Alw26I GTCTC 3 cut(s) 669, 1091, 1275
AlwI GGATC 4 cut(s) 840, 1120, 1133, 1406
AlwNI CAGNNNCTG 3 cut(s) 692, 1142, 1562
Aor13HI TCCGGA 1 cut(s) 217
AoxI GGCC 3 cut(s) 573, 1011, 1138
ApeKI GCWGC 3 cut(s) 692, 1506, 1541
ApoI RAATTY 4 cut(s) 40, 294, 528, 1077
Asp700I GAANNNNTTC 1 cut(s) 552
AspLEI GCGC 1 cut(s) 867
AspS9I GGNCC 4 cut(s) 714, 758, 859, 1139
AsuHPI GGTGA 3 cut(s) 1202, 1391, 1577
AsuII TTCGAA 2 cut(s) 233, 556
AsuNHI GCTAGC 1 cut(s) 914
AvaII GGWCC 3 cut(s) 714, 758, 859
BaeI ACNNNNGTAYC 2 cut(s) 894, 927
BalI TGGCCA 1 cut(s) 1013
BamHI GGATCC 1 cut(s) 1125
Bbv12I GWGCWC 1 cut(s) 375
BbvI GCAGC 3 cut(s) 679, 1518, 1553
BccI CCATC 3 cut(s) 399, 1049, 1187
BcgI CGANNNNNNTGC 2 cut(s) 1337, 1371
BclI TGATCA 1 cut(s) 1525
BcoDI GTCTC 3 cut(s) 669, 1091, 1275
BfaI CTAG 6 cut(s) 56, 198, 323, 915, 951, 1236
BfmI CTRYAG 3 cut(s) 355, 693, 906
BisI GCNGC 5 cut(s) 420, 693, 1481, 1507, 1542
BlsI GCNGC 5 cut(s) 421, 694, 1482, 1508, 1543
Bme18I GGWCC 3 cut(s) 714, 758, 859
BmgT120I GGNCC 4 cut(s) 714, 758, 859, 1139
BmiI GGNNCC 4 cut(s) 423, 715, 759, 1127
BmsI GCATC 4 cut(s) 350, 374, 437, 760
BmtI GCTAGC 1 cut(s) 918
BpmI CTGGAG 3 cut(s) 261, 408, 1442
Bpu14I TTCGAA 2 cut(s) 233, 556
BpuEI CTTGAG 1 cut(s) 1115
BsaI GGTCTC 2 cut(s) 669, 1275
BsaJI CCNNGG 4 cut(s) 432, 576, 1008, 1169
BsaWI WCCGGW 2 cut(s) 217, 1043
Bsc4I CCNNNNNNNGG 4 cut(s) 723, 1027, 1136, 1562
Bse1I ACTGG 3 cut(s) 191, 1556, 1567
BseAI TCCGGA 1 cut(s) 217
BseDI CCNNGG 4 cut(s) 432, 576, 1008, 1169
BseGI GGATG 3 cut(s) 428, 937, 1479
BseLI CCNNNNNNNGG 4 cut(s) 723, 1027, 1136, 1562
BseMII CTCAG 8 cut(s) 18, 360, 723, 1073, 1149, 1356, 1548, 1601
BseNI ACTGG 3 cut(s) 191, 1556, 1567
BseRI GAGGAG 1 cut(s) 1328
BseXI GCAGC 3 cut(s) 679, 1518, 1553
BsgI GTGCAG 1 cut(s) 705
BshFI GGCC 3 cut(s) 575, 1013, 1140
BsiHKAI GWGCWC 1 cut(s) 375
BsiSI CCGG 2 cut(s) 218, 1044
BslFI GGGAC 4 cut(s) 423, 525, 959, 1267
BslI CCNNNNNNNGG 4 cut(s) 723, 1027, 1136, 1562
BsmAI GTCTC 3 cut(s) 669, 1091, 1275
BsmFI GGGAC 4 cut(s) 423, 525, 959, 1267
BsmI GAATGC 3 cut(s) 17, 1147, 1317
BsnI GGCC 3 cut(s) 575, 1013, 1140
Bso31I GGTCTC 2 cut(s) 669, 1275
Bsp119I TTCGAA 2 cut(s) 233, 556
Bsp1286I GDGCHC 1 cut(s) 375
Bsp13I TCCGGA 1 cut(s) 217
Bsp143I GATC 7 cut(s) 253, 585, 832, 1125, 1360, 1398, 1525
Bsp19I CCATGG 1 cut(s) 1169
BspACI CCGC 3 cut(s) 65, 419, 1480
BspANI GGCC 3 cut(s) 575, 1013, 1140
BspCNI CTCAG 8 cut(s) 19, 361, 724, 1074, 1148, 1355, 1547, 1602
BspEI TCCGGA 1 cut(s) 217
BspHI TCATGA 1 cut(s) 898
BspLI GGNNCC 4 cut(s) 423, 715, 759, 1127
BspMAI CTGCAG 1 cut(s) 697
BspOI GCTAGC 1 cut(s) 918
BspPI GGATC 4 cut(s) 840, 1120, 1133, 1406
BspT104I TTCGAA 2 cut(s) 233, 556
BspTNI GGTCTC 2 cut(s) 669, 1275
BsrBI CCGCTC 1 cut(s) 67
BsrI ACTGG 3 cut(s) 191, 1556, 1567
BssECI CCNNGG 4 cut(s) 432, 576, 1008, 1169
BssMI GATC 7 cut(s) 253, 585, 832, 1125, 1360, 1398, 1525
BssT1I CCWWGG 3 cut(s) 432, 1008, 1169
Bst4CI ACNGT 7 cut(s) 516, 562, 616, 800, 820, 910, 1586
BstAPI GCANNNNNTGC 2 cut(s) 692, 1002
BstBI TTCGAA 2 cut(s) 233, 556
BstC8I GCNNGC 4 cut(s) 363, 916, 1245, 1607
BstDEI CTNAG 9 cut(s) 27, 36, 369, 732, 1082, 1135, 1342, 1534, 1610
BstDSI CCRYGG 1 cut(s) 1169
BstENI CCTNNNNNAGG 1 cut(s) 1134
BstF5I GGATG 3 cut(s) 428, 937, 1479
BstHHI GCGC 1 cut(s) 867
BstKTI GATC 7 cut(s) 256, 588, 835, 1128, 1363, 1401, 1528
BstMAI GTCTC 3 cut(s) 669, 1091, 1275
BstMBI GATC 7 cut(s) 253, 585, 832, 1125, 1360, 1398, 1525
BstMWI GCNNNNNNNGC 5 cut(s) 425, 692, 920, 1002, 1486
BstNSI RCATGY 1 cut(s) 1072
BstSFI CTRYAG 3 cut(s) 355, 693, 906
BstV1I GCAGC 3 cut(s) 679, 1518, 1553
BstX2I RGATCY 2 cut(s) 1125, 1398
BstYI RGATCY 2 cut(s) 1125, 1398
BsuRI GGCC 3 cut(s) 575, 1013, 1140
BtgI CCRYGG 1 cut(s) 1169
BtsCI GGATG 3 cut(s) 428, 937, 1479
BtsI GCAGTG 1 cut(s) 961
BtsIMutI CAGTG 6 cut(s) 264, 805, 842, 961, 1521, 1560
Cac8I GCNNGC 4 cut(s) 363, 916, 1245, 1607
CaiI CAGNNNCTG 3 cut(s) 692, 1142, 1562
CciI TCATGA 1 cut(s) 898
CfoI GCGC 1 cut(s) 867
Cfr13I GGNCC 4 cut(s) 714, 758, 859, 1139
DdeI CTNAG 9 cut(s) 27, 36, 369, 732, 1082, 1135, 1342, 1534, 1610
DpnI GATC 7 cut(s) 255, 587, 834, 1127, 1362, 1400, 1527
DpnII GATC 7 cut(s) 253, 585, 832, 1125, 1360, 1398, 1525
DraI TTTAAA 1 cut(s) 1230
EaeI YGGCCR 1 cut(s) 1011
Eco130I CCWWGG 3 cut(s) 432, 1008, 1169
Eco31I GGTCTC 2 cut(s) 669, 1275
Eco32I GATATC 1 cut(s) 288
Eco47I GGWCC 3 cut(s) 714, 758, 859
Eco57I CTGAAG 3 cut(s) 126, 866, 1233
EcoNI CCTNNNNNAGG 1 cut(s) 1134
EcoO109I RGGNCCY 4 cut(s) 714, 758, 859, 1139
EcoRV GATATC 1 cut(s) 288
EcoT14I CCWWGG 3 cut(s) 432, 1008, 1169
ErhI CCWWGG 3 cut(s) 432, 1008, 1169
FaqI GGGAC 4 cut(s) 423, 525, 959, 1267
FbaI TGATCA 1 cut(s) 1525
FblI GTMKAC 1 cut(s) 1347
Fnu4HI GCNGC 5 cut(s) 420, 693, 1481, 1507, 1542
FokI GGATG 3 cut(s) 415, 944, 1486
Fsp4HI GCNGC 5 cut(s) 420, 693, 1481, 1507, 1542
FspBI CTAG 6 cut(s) 56, 198, 323, 915, 951, 1236
GlaI GCGC 1 cut(s) 866
GluI GCNGC 5 cut(s) 420, 693, 1481, 1507, 1542
GsuI CTGGAG 3 cut(s) 261, 408, 1442
HaeIII GGCC 3 cut(s) 575, 1013, 1140
HapII CCGG 2 cut(s) 218, 1044
HhaI GCGC 1 cut(s) 867
Hin6I GCGC 1 cut(s) 865
HinP1I GCGC 1 cut(s) 865
HincII GTYRAC 2 cut(s) 249, 1348
HindII GTYRAC 2 cut(s) 249, 1348
HindIII AAGCTT 2 cut(s) 1223, 1274
HinfI GANTC 8 cut(s) 50, 83, 146, 221, 961, 1084, 1333, 1552
HpaII CCGG 2 cut(s) 218, 1044
HphI GGTGA 3 cut(s) 1202, 1391, 1577
Hpy166II GTNNAC 3 cut(s) 249, 1348, 1589
Hpy188I TCNGA 9 cut(s) 71, 145, 292, 300, 370, 451, 931, 966, 1083
Hpy188III TCNNGA 6 cut(s) 56, 218, 441, 899, 1402, 1421
Hpy8I GTNNAC 3 cut(s) 249, 1348, 1589
HpyAV CCTTC 6 cut(s) 281, 379, 460, 632, 1379, 1428
HpyCH4III ACNGT 7 cut(s) 516, 562, 616, 800, 820, 910, 1586
HpyCH4IV ACGT 2 cut(s) 596, 1591
HpyF10VI GCNNNNNNNGC 5 cut(s) 425, 692, 920, 1002, 1486
HpyF3I CTNAG 9 cut(s) 27, 36, 369, 732, 1082, 1135, 1342, 1534, 1610
HpySE526I ACGT 2 cut(s) 596, 1591
HspAI GCGC 1 cut(s) 865
Kpn2I TCCGGA 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 1525
Kzo9I GATC 7 cut(s) 253, 585, 832, 1125, 1360, 1398, 1525
LmnI GCTCC 5 cut(s) 427, 495, 748, 1010, 1494
Lsp1109I GCAGC 3 cut(s) 679, 1518, 1553
LweI GCATC 4 cut(s) 350, 374, 437, 760
MaeI CTAG 6 cut(s) 56, 198, 323, 915, 951, 1236
MaeII ACGT 2 cut(s) 596, 1591
MaeIII GTNAC 1 cut(s) 1558
MalI GATC 7 cut(s) 255, 587, 834, 1127, 1362, 1400, 1527
MbiI CCGCTC 1 cut(s) 67
MboI GATC 7 cut(s) 253, 585, 832, 1125, 1360, 1398, 1525
MboII GAAGA 4 cut(s) 647, 648, 1064, 1561
MflI RGATCY 2 cut(s) 1125, 1398
MhlI GDGCHC 1 cut(s) 375
MlsI TGGCCA 1 cut(s) 1013
MluNI TGGCCA 1 cut(s) 1013
MlyI GAGTC 3 cut(s) 44, 215, 1093
MmeI TCCRAC 1 cut(s) 759
Mox20I TGGCCA 1 cut(s) 1013
MroI TCCGGA 1 cut(s) 217
MroXI GAANNNNTTC 1 cut(s) 552
MscI TGGCCA 1 cut(s) 1013
MseI TTAA 4 cut(s) 855, 1229, 1272, 1448
MslI CAYNNNNRTG 3 cut(s) 10, 847, 1071
Msp20I TGGCCA 1 cut(s) 1013
MspA1I CMGCKG 2 cut(s) 419, 923
MspI CCGG 2 cut(s) 218, 1044
Mva1269I GAATGC 3 cut(s) 17, 1147, 1317
MwoI GCNNNNNNNGC 5 cut(s) 425, 692, 920, 1002, 1486
NcoI CCATGG 1 cut(s) 1169
NdeII GATC 7 cut(s) 253, 585, 832, 1125, 1360, 1398, 1525
NheI GCTAGC 1 cut(s) 914
NlaIV GGNNCC 4 cut(s) 423, 715, 759, 1127
NmeAIII GCCGAG 1 cut(s) 557
NmuCI GTSAC 1 cut(s) 1558
NspI RCATGY 1 cut(s) 1072
NspV TTCGAA 2 cut(s) 233, 556
OliI CACNNNNGTG 1 cut(s) 847
PagI TCATGA 1 cut(s) 898
PciI ACATGT 1 cut(s) 1068
PctI GAATGC 3 cut(s) 17, 1147, 1317
PdmI GAANNNNTTC 1 cut(s) 552
PfeI GAWTC 5 cut(s) 83, 146, 961, 1333, 1552
PflMI CCANNNNNTGG 1 cut(s) 1562
PkrI GCNGC 5 cut(s) 421, 694, 1482, 1508, 1543
PleI GAGTC 3 cut(s) 44, 215, 1092
PpsI GAGTC 3 cut(s) 44, 215, 1092
PpuMI RGGWCCY 3 cut(s) 714, 758, 859
PscI ACATGT 1 cut(s) 1068
Psp5II RGGWCCY 3 cut(s) 714, 758, 859
PspN4I GGNNCC 4 cut(s) 423, 715, 759, 1127
PspPI GGNCC 4 cut(s) 714, 758, 859, 1139
PspPPI RGGWCCY 3 cut(s) 714, 758, 859
PstI CTGCAG 1 cut(s) 697
PstNI CAGNNNCTG 3 cut(s) 692, 1142, 1562
PsuI RGATCY 2 cut(s) 1125, 1398
PvuII CAGCTG 1 cut(s) 923
RseI CAYNNNNRTG 3 cut(s) 10, 847, 1071
SalI GTCGAC 1 cut(s) 1346
SaqAI TTAA 4 cut(s) 855, 1229, 1272, 1448
SatI GCNGC 5 cut(s) 420, 693, 1481, 1507, 1542
Sau3AI GATC 7 cut(s) 253, 585, 832, 1125, 1360, 1398, 1525
Sau96I GGNCC 4 cut(s) 714, 758, 859, 1139
SchI GAGTC 3 cut(s) 44, 215, 1093
SduI GDGCHC 1 cut(s) 375
SfaNI GCATC 4 cut(s) 350, 374, 437, 760
SfcI CTRYAG 3 cut(s) 355, 693, 906
SfuI TTCGAA 2 cut(s) 233, 556
SinI GGWCC 3 cut(s) 714, 758, 859
SmiMI CAYNNNNRTG 3 cut(s) 10, 847, 1071
SmlI CTYRAG 1 cut(s) 1094
SmoI CTYRAG 1 cut(s) 1094
SsiI CCGC 3 cut(s) 65, 419, 1480
SspI AATATT 1 cut(s) 1109
SspMI CTAG 6 cut(s) 56, 198, 323, 915, 951, 1236
StyI CCWWGG 3 cut(s) 432, 1008, 1169
TaaI ACNGT 7 cut(s) 516, 562, 616, 800, 820, 910, 1586
TaiI ACGT 2 cut(s) 599, 1594
TaqI TCGA 7 cut(s) 48, 233, 344, 480, 556, 835, 1347
TaqII GACCGA 1 cut(s) 246
TauI GCSGC 2 cut(s) 422, 1483
TfiI GAWTC 5 cut(s) 83, 146, 961, 1333, 1552
Tru1I TTAA 4 cut(s) 855, 1229, 1272, 1448
Tru9I TTAA 4 cut(s) 855, 1229, 1272, 1448
TscAI CASTG 6 cut(s) 271, 805, 849, 961, 1528, 1567
TseFI GTSAC 1 cut(s) 1558
TseI GCWGC 3 cut(s) 692, 1506, 1541
Tsp45I GTSAC 1 cut(s) 1558
TspDTI ATGAA 8 cut(s) 34, 86, 138, 324, 968, 1089, 1119, 1254
TspGWI ACGGA 1 cut(s) 671
TspRI CASTG 6 cut(s) 271, 805, 849, 961, 1528, 1567
Van91I CCANNNNNTGG 1 cut(s) 1562
VpaK11BI GGWCC 3 cut(s) 714, 758, 859
XagI CCTNNNNNAGG 1 cut(s) 1134
XapI RAATTY 4 cut(s) 40, 294, 528, 1077
XbaI TCTAGA 1 cut(s) 55
XceI RCATGY 1 cut(s) 1072
XmiI GTMKAC 1 cut(s) 1347
XmnI GAANNNNTTC 1 cut(s) 552
XspI CTAG 6 cut(s) 56, 198, 323, 915, 951, 1236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.