Rroxscaffold_1G00031720

F-box WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007918
Physical Location & Seq
Reverse (-)
44968142 .. 44970554
2413 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00031720.1

Sequence Viewer

Length: 1686 bp
ATGGCATTTGAATGCCGAGCGAATACTGAGGTTTCTAAGAATTTGATCGACTCTCTAGACAATCCGCTAGCTGAATCTAATCAATTTCATTCTCAAATTGAAGCTGAAAATTTTGGTGTTTCAAAAGTAGCTTCAGATTCATATACTAAAAAAGAAAAATGTGTAATTTCAAAACCCAAACCAGTTTCTAGTTCCAAGAAAGCAAATCCGGACTCTTGTGGTTCGAAACAGCTTTTGTCAACTGATCGGTCAATCACCGACCTTCCTCCAGCTTTGATATCCGAAATTCTGAATTGCCTTGACCCGAAAGAGCTAGGCATAGTTTCATGTGCATCGACATATCTCTGTAGGCTCGCATCTGAGCACCATGTTTGGAAGGAGTTTTATTGTGAGAGATGGGGACTTTCAGCGGCTCCAGCATCCTTGGGTTCAGGACTTTCTGATGACAAGTCATGGAAGGAGTTTTTTGTCGAAAGGGAGTTTAGGAGTAAAACTTTTATGGGACGGTATAGCATGGAAATTTTGTATGGTCATACTGAACCTGTTCGAACAGTTTTCCTTTTGGCCTCGGCAAAGCTCATATTTACGTCAGGTTATGACCAAACCGTAAAAATGTGGAACATGGAAGAAGGGCTGTTGATTGCTTCTTCAAGGTCTCTTGGTTGCACTATCCGTGCAGTTGCTGCAGATACAAAACTCTTGGTTGCTGGTGGCACTGATGGTTTTATCCACTGTTGGAAGGCAATAGAAGGGAGTCCACACTTGTTTAATATCAGGGGTCCTCAAAATGAGAATACTGAGTTTCGTCTGTGGGAGCATCAGGGTCCTATAACATCTCTTGCGTTGGATTTTACAAGGATTTATGGTGGTTCATGGGATATGACTGTTCGTGTATGGGATCGACTTTCACTGAAGTGTGTTAAGGTCCTGCGCCATAGTGATTGGGTTGGGGGAATTGCACCTCATGATACTACAGTTGCTAGCACAGCTGGTTCGGATGTCTATGTTTGGGACACTAGCAGTGGGAAGCTGATGACCATTATTCATAATGCTCATGTTGGCAAAACTTGCTCCTTGGCCAGAAACCACAAAGGGGATTTTCTATTTACCGGAGGGGAAGATGGTGCAATTCACATGTATGAAATTCTGAGTCATGGGCTTGAGACTAATGAATATTTGGTTGCAACATGGATCCCTCACTCAGGCCCTGTGCATTCCCTTGCGTTTGAGTTTCCATGGCTTGTTTCTGCTTCTGGTGATGGGAAGCTATCACTAATTGATGTGAGAAAGCTTTTAAAAACTAGAAAGCGTGCTTCAGGGACAAGTGCTTCATGTGTTAAGCATGGAGACCAGAGTAGTGTAGAACCCCCACAGAGAATGCTACATGGATTTGCAAAGAATCTCCTCTCAGTTGACATTGGTGCTGATCGTATTGTATGTGGGGGTGAGGAAGGTGTTGTTAGGATCTGGAACTTCACACAAGCTCTGGAGATTGAGCGAAGGGCTAATGCTTTAAGAGGAATACGATTAGAGAATAGGATGAGGCGGCGTAAGCTCCATGTAGAGAATAGCAGCAAAGGTGGTCGCACTGATCAATGCTCAGTTGCAGCCAAGAAGAATCCAGTCACTGGTGAAAGGAATGGTTGGCACAGTAAACGTGGGGCGAGTGGCAAGCTGAGAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0050896 GO:0051716 GO:0071496
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Lipase_GDSL WD40

Protein Analysis

561

Amino Acids

62.06

Weight (kDa)

8.79

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 85 - 130 1e-06 F-box-like
Beta-prop_WDR5 PF25175 147 - 251 2.2e-07 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 173 - 247 3.9e-07 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 218 - 439 2.6e-11 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 229 - 435 3.8e-12 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 283 - 494 1.2e-12 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 287 - 430 5.9e-07 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 325 - 429 1.3e-08 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 325 - 431 6.6e-07 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1628
AccIII TCCGGA 1 cut(s) 208
AciI CCGC 3 cut(s) 65, 410, 1546
AclWI GGATC 4 cut(s) 906, 1186, 1199, 1472
AcoI YGGCCR 1 cut(s) 1077
AcsI RAATTY 5 cut(s) 40, 109, 285, 519, 1143
AcuI CTGAAG 3 cut(s) 117, 932, 1299
AfiI CCNNNNNNNGG 3 cut(s) 1093, 1202, 1628
AflIII ACRYGT 1 cut(s) 1134
AgsI TTSAA 5 cut(s) 11, 101, 123, 171, 651
AleI CACNNNNGTG 1 cut(s) 913
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 3 cut(s) 660, 1157, 1341
AlwI GGATC 4 cut(s) 906, 1186, 1199, 1472
AlwNI CAGNNNCTG 3 cut(s) 683, 1208, 1628
Aor13HI TCCGGA 1 cut(s) 208
AoxI GGCC 3 cut(s) 564, 1077, 1204
ApeKI GCWGC 3 cut(s) 683, 1572, 1607
ApoI RAATTY 5 cut(s) 40, 109, 285, 519, 1143
Asp700I GAANNNNTTC 1 cut(s) 543
AspLEI GCGC 1 cut(s) 933
AspS9I GGNCC 4 cut(s) 779, 824, 925, 1205
AsuHPI GGTGA 4 cut(s) 247, 1268, 1457, 1643
AsuII TTCGAA 2 cut(s) 224, 547
AsuNHI GCTAGC 2 cut(s) 67, 980
AvaII GGWCC 3 cut(s) 779, 824, 925
BaeI ACNNNNGTAYC 2 cut(s) 960, 993
BalI TGGCCA 1 cut(s) 1079
BamHI GGATCC 1 cut(s) 1191
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 3 cut(s) 670, 1584, 1619
BccI CCATC 4 cut(s) 390, 713, 1115, 1253
BclI TGATCA 1 cut(s) 1591
BcoDI GTCTC 3 cut(s) 660, 1157, 1341
BfaI CTAG 7 cut(s) 56, 68, 189, 314, 981, 1017, 1302
BfmI CTRYAG 3 cut(s) 346, 684, 972
BisI GCNGC 5 cut(s) 411, 684, 1547, 1573, 1608
BlsI GCNGC 5 cut(s) 412, 685, 1548, 1574, 1609
Bme18I GGWCC 3 cut(s) 779, 824, 925
BmgT120I GGNCC 4 cut(s) 779, 824, 925, 1205
BmiI GGNNCC 4 cut(s) 414, 780, 825, 1193
BmsI GCATC 4 cut(s) 341, 365, 428, 826
BmtI GCTAGC 2 cut(s) 71, 984
BpmI CTGGAG 3 cut(s) 252, 399, 1508
Bpu14I TTCGAA 2 cut(s) 224, 547
BpuEI CTTGAG 1 cut(s) 1181
BsaI GGTCTC 2 cut(s) 660, 1341
BsaJI CCNNGG 4 cut(s) 423, 567, 1074, 1235
BsaWI WCCGGW 2 cut(s) 208, 1109
Bsc4I CCNNNNNNNGG 3 cut(s) 1093, 1202, 1628
Bse1I ACTGG 3 cut(s) 182, 1622, 1633
BseAI TCCGGA 1 cut(s) 208
BseDI CCNNGG 4 cut(s) 423, 567, 1074, 1235
BseGI GGATG 3 cut(s) 419, 1003, 1545
BseLI CCNNNNNNNGG 3 cut(s) 1093, 1202, 1628
BseMII CTCAG 8 cut(s) 18, 351, 789, 1139, 1215, 1422, 1614, 1667
BseNI ACTGG 3 cut(s) 182, 1622, 1633
BseRI GAGGAG 1 cut(s) 1394
BseXI GCAGC 3 cut(s) 670, 1584, 1619
BsgI GTGCAG 1 cut(s) 696
BshFI GGCC 3 cut(s) 566, 1079, 1206
BsiHKAI GWGCWC 1 cut(s) 366
BsiSI CCGG 2 cut(s) 209, 1110
BslFI GGGAC 4 cut(s) 414, 516, 1025, 1333
BslI CCNNNNNNNGG 3 cut(s) 1093, 1202, 1628
BsmAI GTCTC 3 cut(s) 660, 1157, 1341
BsmFI GGGAC 4 cut(s) 414, 516, 1025, 1333
BsmI GAATGC 3 cut(s) 17, 1213, 1383
BsnI GGCC 3 cut(s) 566, 1079, 1206
Bso31I GGTCTC 2 cut(s) 660, 1341
Bsp119I TTCGAA 2 cut(s) 224, 547
Bsp1286I GDGCHC 1 cut(s) 366
Bsp13I TCCGGA 1 cut(s) 208
Bsp143I GATC 7 cut(s) 45, 244, 898, 1191, 1426, 1464, 1591
Bsp19I CCATGG 1 cut(s) 1235
BspACI CCGC 3 cut(s) 65, 410, 1546
BspANI GGCC 3 cut(s) 566, 1079, 1206
BspCNI CTCAG 8 cut(s) 19, 352, 790, 1140, 1214, 1421, 1613, 1668
BspEI TCCGGA 1 cut(s) 208
BspHI TCATGA 1 cut(s) 964
BspLI GGNNCC 4 cut(s) 414, 780, 825, 1193
BspMAI CTGCAG 1 cut(s) 688
BspOI GCTAGC 2 cut(s) 71, 984
BspPI GGATC 4 cut(s) 906, 1186, 1199, 1472
BspT104I TTCGAA 2 cut(s) 224, 547
BspTNI GGTCTC 2 cut(s) 660, 1341
BsrI ACTGG 3 cut(s) 182, 1622, 1633
BssECI CCNNGG 4 cut(s) 423, 567, 1074, 1235
BssMI GATC 7 cut(s) 45, 244, 898, 1191, 1426, 1464, 1591
BssT1I CCWWGG 3 cut(s) 423, 1074, 1235
Bst4CI ACNGT 7 cut(s) 507, 553, 607, 734, 886, 976, 1652
BstAPI GCANNNNNTGC 2 cut(s) 683, 1068
BstBI TTCGAA 2 cut(s) 224, 547
BstC8I GCNNGC 5 cut(s) 69, 354, 982, 1311, 1673
BstDEI CTNAG 9 cut(s) 27, 36, 360, 798, 1148, 1201, 1408, 1600, 1676
BstDSI CCRYGG 1 cut(s) 1235
BstENI CCTNNNNNAGG 1 cut(s) 1200
BstF5I GGATG 3 cut(s) 419, 1003, 1545
BstHHI GCGC 1 cut(s) 933
BstKTI GATC 7 cut(s) 48, 247, 901, 1194, 1429, 1467, 1594
BstMAI GTCTC 3 cut(s) 660, 1157, 1341
BstMBI GATC 7 cut(s) 45, 244, 898, 1191, 1426, 1464, 1591
BstMWI GCNNNNNNNGC 5 cut(s) 416, 683, 986, 1068, 1552
BstNSI RCATGY 1 cut(s) 1138
BstSFI CTRYAG 3 cut(s) 346, 684, 972
BstV1I GCAGC 3 cut(s) 670, 1584, 1619
BstX2I RGATCY 2 cut(s) 1191, 1464
BstYI RGATCY 2 cut(s) 1191, 1464
BsuRI GGCC 3 cut(s) 566, 1079, 1206
BtgI CCRYGG 1 cut(s) 1235
BtsCI GGATG 3 cut(s) 419, 1003, 1545
BtsI GCAGTG 1 cut(s) 1027
BtsIMutI CAGTG 6 cut(s) 714, 730, 908, 1027, 1587, 1626
Cac8I GCNNGC 5 cut(s) 69, 354, 982, 1311, 1673
CaiI CAGNNNCTG 3 cut(s) 683, 1208, 1628
CciI TCATGA 1 cut(s) 964
CfoI GCGC 1 cut(s) 933
Cfr13I GGNCC 4 cut(s) 779, 824, 925, 1205
DdeI CTNAG 9 cut(s) 27, 36, 360, 798, 1148, 1201, 1408, 1600, 1676
DpnI GATC 7 cut(s) 47, 246, 900, 1193, 1428, 1466, 1593
DpnII GATC 7 cut(s) 45, 244, 898, 1191, 1426, 1464, 1591
DraI TTTAAA 1 cut(s) 1296
EaeI YGGCCR 1 cut(s) 1077
Eco130I CCWWGG 3 cut(s) 423, 1074, 1235
Eco31I GGTCTC 2 cut(s) 660, 1341
Eco32I GATATC 1 cut(s) 279
Eco47I GGWCC 3 cut(s) 779, 824, 925
Eco57I CTGAAG 3 cut(s) 117, 932, 1299
EcoNI CCTNNNNNAGG 1 cut(s) 1200
EcoO109I RGGNCCY 4 cut(s) 779, 824, 925, 1205
EcoRV GATATC 1 cut(s) 279
EcoT14I CCWWGG 3 cut(s) 423, 1074, 1235
ErhI CCWWGG 3 cut(s) 423, 1074, 1235
FaqI GGGAC 4 cut(s) 414, 516, 1025, 1333
FbaI TGATCA 1 cut(s) 1591
Fnu4HI GCNGC 5 cut(s) 411, 684, 1547, 1573, 1608
FokI GGATG 3 cut(s) 406, 1010, 1552
Fsp4HI GCNGC 5 cut(s) 411, 684, 1547, 1573, 1608
FspBI CTAG 7 cut(s) 56, 68, 189, 314, 981, 1017, 1302
GlaI GCGC 1 cut(s) 932
GluI GCNGC 5 cut(s) 411, 684, 1547, 1573, 1608
GsuI CTGGAG 3 cut(s) 252, 399, 1508
HaeIII GGCC 3 cut(s) 566, 1079, 1206
HapII CCGG 2 cut(s) 209, 1110
HhaI GCGC 1 cut(s) 933
Hin6I GCGC 1 cut(s) 931
HinP1I GCGC 1 cut(s) 931
HincII GTYRAC 2 cut(s) 240, 1414
HindII GTYRAC 2 cut(s) 240, 1414
HindIII AAGCTT 1 cut(s) 1289
HinfI GANTC 8 cut(s) 50, 74, 137, 212, 754, 1150, 1399, 1618
HpaII CCGG 2 cut(s) 209, 1110
HphI GGTGA 4 cut(s) 247, 1268, 1457, 1643
Hpy166II GTNNAC 4 cut(s) 240, 758, 1414, 1655
Hpy188I TCNGA 7 cut(s) 136, 283, 291, 361, 442, 997, 1149
Hpy188III TCNNGA 6 cut(s) 56, 209, 432, 965, 1468, 1487
Hpy8I GTNNAC 4 cut(s) 240, 758, 1414, 1655
HpyAV CCTTC 8 cut(s) 272, 370, 451, 623, 733, 743, 1445, 1494
HpyCH4III ACNGT 7 cut(s) 507, 553, 607, 734, 886, 976, 1652
HpyCH4IV ACGT 2 cut(s) 587, 1657
HpyF10VI GCNNNNNNNGC 5 cut(s) 416, 683, 986, 1068, 1552
HpyF3I CTNAG 9 cut(s) 27, 36, 360, 798, 1148, 1201, 1408, 1600, 1676
HpySE526I ACGT 2 cut(s) 587, 1657
HspAI GCGC 1 cut(s) 931
Kpn2I TCCGGA 1 cut(s) 208
Ksp22I TGATCA 1 cut(s) 1591
Kzo9I GATC 7 cut(s) 45, 244, 898, 1191, 1426, 1464, 1591
LmnI GCTCC 4 cut(s) 418, 814, 1076, 1560
Lsp1109I GCAGC 3 cut(s) 670, 1584, 1619
LweI GCATC 4 cut(s) 341, 365, 428, 826
MaeI CTAG 7 cut(s) 56, 68, 189, 314, 981, 1017, 1302
MaeII ACGT 2 cut(s) 587, 1657
MaeIII GTNAC 1 cut(s) 1624
MalI GATC 7 cut(s) 47, 246, 900, 1193, 1428, 1466, 1593
MboI GATC 7 cut(s) 45, 244, 898, 1191, 1426, 1464, 1591
MboII GAAGA 4 cut(s) 638, 639, 1130, 1627
MflI RGATCY 2 cut(s) 1191, 1464
MhlI GDGCHC 1 cut(s) 366
MlsI TGGCCA 1 cut(s) 1079
MluNI TGGCCA 1 cut(s) 1079
MlyI GAGTC 4 cut(s) 44, 206, 763, 1159
MmeI TCCRAC 2 cut(s) 716, 825
Mox20I TGGCCA 1 cut(s) 1079
MroI TCCGGA 1 cut(s) 208
MroXI GAANNNNTTC 1 cut(s) 543
MscI TGGCCA 1 cut(s) 1079
MseI TTAA 5 cut(s) 768, 921, 1295, 1338, 1514
MslI CAYNNNNRTG 3 cut(s) 10, 913, 1137
Msp20I TGGCCA 1 cut(s) 1079
MspA1I CMGCKG 2 cut(s) 410, 989
MspI CCGG 2 cut(s) 209, 1110
Mva1269I GAATGC 3 cut(s) 17, 1213, 1383
MwoI GCNNNNNNNGC 5 cut(s) 416, 683, 986, 1068, 1552
NcoI CCATGG 1 cut(s) 1235
NdeII GATC 7 cut(s) 45, 244, 898, 1191, 1426, 1464, 1591
NheI GCTAGC 2 cut(s) 67, 980
NlaIV GGNNCC 4 cut(s) 414, 780, 825, 1193
NmeAIII GCCGAG 2 cut(s) 41, 548
NmuCI GTSAC 1 cut(s) 1624
NspI RCATGY 1 cut(s) 1138
NspV TTCGAA 2 cut(s) 224, 547
OliI CACNNNNGTG 1 cut(s) 913
PagI TCATGA 1 cut(s) 964
PciI ACATGT 1 cut(s) 1134
PctI GAATGC 3 cut(s) 17, 1213, 1383
PdmI GAANNNNTTC 1 cut(s) 543
PfeI GAWTC 4 cut(s) 74, 137, 1399, 1618
PflMI CCANNNNNTGG 1 cut(s) 1628
PkrI GCNGC 5 cut(s) 412, 685, 1548, 1574, 1609
PleI GAGTC 4 cut(s) 44, 206, 762, 1158
PpsI GAGTC 4 cut(s) 44, 206, 762, 1158
PpuMI RGGWCCY 3 cut(s) 779, 824, 925
PscI ACATGT 1 cut(s) 1134
Psp5II RGGWCCY 3 cut(s) 779, 824, 925
PspN4I GGNNCC 4 cut(s) 414, 780, 825, 1193
PspPI GGNCC 4 cut(s) 779, 824, 925, 1205
PspPPI RGGWCCY 3 cut(s) 779, 824, 925
PstI CTGCAG 1 cut(s) 688
PstNI CAGNNNCTG 3 cut(s) 683, 1208, 1628
PsuI RGATCY 2 cut(s) 1191, 1464
PvuII CAGCTG 1 cut(s) 989
RseI CAYNNNNRTG 3 cut(s) 10, 913, 1137
SaqAI TTAA 5 cut(s) 768, 921, 1295, 1338, 1514
SatI GCNGC 5 cut(s) 411, 684, 1547, 1573, 1608
Sau3AI GATC 7 cut(s) 45, 244, 898, 1191, 1426, 1464, 1591
Sau96I GGNCC 4 cut(s) 779, 824, 925, 1205
SchI GAGTC 4 cut(s) 44, 206, 763, 1159
SduI GDGCHC 1 cut(s) 366
SfaNI GCATC 4 cut(s) 341, 365, 428, 826
SfcI CTRYAG 3 cut(s) 346, 684, 972
SfuI TTCGAA 2 cut(s) 224, 547
SinI GGWCC 3 cut(s) 779, 824, 925
SmiMI CAYNNNNRTG 3 cut(s) 10, 913, 1137
SmlI CTYRAG 1 cut(s) 1160
SmoI CTYRAG 1 cut(s) 1160
SsiI CCGC 3 cut(s) 65, 410, 1546
SspI AATATT 1 cut(s) 1175
SspMI CTAG 7 cut(s) 56, 68, 189, 314, 981, 1017, 1302
StyI CCWWGG 3 cut(s) 423, 1074, 1235
TaaI ACNGT 7 cut(s) 507, 553, 607, 734, 886, 976, 1652
TaiI ACGT 2 cut(s) 590, 1660
TaqI TCGA 6 cut(s) 48, 224, 335, 471, 547, 901
TaqII GACCGA 1 cut(s) 237
TauI GCSGC 2 cut(s) 413, 1549
TfiI GAWTC 4 cut(s) 74, 137, 1399, 1618
Tru1I TTAA 5 cut(s) 768, 921, 1295, 1338, 1514
Tru9I TTAA 5 cut(s) 768, 921, 1295, 1338, 1514
TscAI CASTG 6 cut(s) 721, 737, 915, 1027, 1594, 1633
TseFI GTSAC 1 cut(s) 1624
TseI GCWGC 3 cut(s) 683, 1572, 1607
Tsp45I GTSAC 1 cut(s) 1624
TspDTI ATGAA 8 cut(s) 77, 129, 315, 861, 1034, 1155, 1185, 1320
TspGWI ACGGA 1 cut(s) 662
TspRI CASTG 6 cut(s) 721, 737, 915, 1027, 1594, 1633
Van91I CCANNNNNTGG 1 cut(s) 1628
VpaK11BI GGWCC 3 cut(s) 779, 824, 925
XagI CCTNNNNNAGG 1 cut(s) 1200
XapI RAATTY 5 cut(s) 40, 109, 285, 519, 1143
XbaI TCTAGA 1 cut(s) 55
XceI RCATGY 1 cut(s) 1138
XmnI GAANNNNTTC 1 cut(s) 543
XspI CTAG 7 cut(s) 56, 68, 189, 314, 981, 1017, 1302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.