MD00G1031900.v1.1

F-box WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0007918
Physical Location & Seq
Reverse (-)
5571268 .. 5574554
3287 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1031900.v1.1.491

Sequence Viewer

Length: 1704 bp
ATGGAATTCGAATGCCAAGTGAGTACTCAGGTTTCTAAGAATTTGGCAGACTCTGTAGAAAATTCGTTTCGACAGCAAACTGAATCGAGTCGATTCAATTCTAACCCTGAAGCCGAAAACTTTAGCATTTCAGGACAAACATTAGATTCGTATCCTGAAAAGGAAGAAACTTCAATTCCAAAATCCACACCAATCAAGAAGCCAAACTCGGACTCTTGTGGTTTGAAACAGTTGTTGCGTAGCGATGTCCTTGTTAATTCTCGTAGAACAATCACCGATCTTCCTCCAGCTTTGTTATCTGAAATTCTGAATTGCCTTGACCCAAAAGAGCTAGGCATAGCTTCATGTGTATCAGCTTATCTCTGTAAGGTTGCATCTGAGCACCATGTTTGGAAGGATTTTTATTGTGAGAGGTGGGGAACTCCCACACTTCCAACACCTTTGGGAAAGGGAGTCTCTGACGAGACATCGTGGAAGGAGTTTTTTGTTGAAAGGGAGTTTAGGAGTAAGACCTTTATGGGACGTTATAGCATTGACGTTATGTATGGTCATACTGAAGCTGTTCAAACAGTTTTCCTTTTGGCTTCCGCAAAGCTCATATTTACTGCTGGGTATGACCAAACTGTAAAAATGTGGAAGATGGAAGAAGGATTATTGATTGCTTCTTCAAGATCTCTTGGTTGCACTATTCGTGCAGTTGCTGGAGATACAAAACTCCTGGTCGCCGGTGGTACTGATGGGTTTATCCAGTGTTGGAGGGCAGTGGAGGGCTTTCCACACTTGTTTGATATTAAGGGTCCTCAAAACCAGAATACCGAGTTTCGGCTATGGGAGCATGAGGGGCCCATAACTTCTCTTGCTTTGGATTTGACTAGGATTTATAGTGGTTCCTGGGACATGACTGTTCGTGTATGGGATCGGTTTTCACTCAAGTGTGAGAAGATTTTGAGGCACAGTGATTGGGTATGGGAACTTGTGCCTTGGGATCTTACAGTTGCTAGCGCATCGGGTTCTGATGTTTATATTTGGGATACTAGTAGCGGGAGACTGATGACCATTATTCATAATGCTCATGTTGGTAATACCTACTCTTTGGCACGAAACCTCACAGGGGATTTTCTATTTTCTGGAGGGGATGACGGTGCAATACACATGTATGAAATCACTAGTCATGGTCTCGAGACTGATGCATTACTCGTTGCCACATGGGTTCCTCACTCAGGCCGTGTCTATTCCCTTGCATTTGAGTTTCCATGGCTTGTTTCTGCATCTGCTGATGGGAAACTCTCACTAATTGATGTGAGAAAGCTTCTAAGGACTAGAAAGCGTGCTTCAGAAAAGGGTTTTTCATGTATTAAGCATGTGGATCCAAGTACTGTGGAACCTCCACAGAGGATGTTACATGGTTTTGCGAAAAATCTTGTGTCAGTGGACATTGGTGCTGATCGTATTGTATGTGGAGGTGAGGAAGGTGTTGTTAGGATATGGAACTTCACAGAAGCTTTGGAGATTGAGCGGAGGGTTTGTGCTTCAAGAGCAATGCGGTTGGAGAATAGGATGAGGCGGCGTATGCTCCATTTAGAGAATAGCAGCAGAGCCAGTCGGAATGATCAATGTTCGGTTGCAGCCAAGAAGAACCCAGTCACTGGTGACAGGAGTGGTTGGCTCAGTAAACGTGCGTTGAGCGGGAAGGTGAAGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0050896 GO:0051716 GO:0071496
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Lipase_GDSL WD40

Protein Analysis

568

Amino Acids

63.63

Weight (kDa)

7.2

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 91 - 136 1.4e-07 F-box-like
F-box PF00646 91 - 132 8.5e-06 F-box domain
Beta-prop_WDR5 PF25175 178 - 256 3.1e-06 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 181 - 253 9.6e-06 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 226 - 447 7.4e-10 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 236 - 441 1.3e-12 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 331 - 437 6.1e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 332 - 435 1.3e-07 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 332 - 436 1.8e-06 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1646
AccBSI CCGCTC 2 cut(s) 1516, 1686
AciI CCGC 6 cut(s) 588, 1041, 1516, 1543, 1564, 1686
AclWI GGATC 4 cut(s) 924, 993, 1361, 1374
AcsI RAATTY 4 cut(s) 5, 40, 61, 303
AcuI CTGAAG 3 cut(s) 129, 576, 1317
AfaI GTAC 3 cut(s) 25, 733, 1375
AfiI CCNNNNNNNGG 4 cut(s) 822, 1111, 1220, 1646
AflIII ACRYGT 1 cut(s) 1152
AgsI TTSAA 7 cut(s) 97, 174, 226, 491, 566, 669, 1533
AhlI ACTAGT 2 cut(s) 1034, 1166
AjnI CCWGG 2 cut(s) 717, 890
AleI CACNNNNGTG 1 cut(s) 931
AluBI AGCT 8 cut(s) 290, 331, 341, 356, 560, 595, 1309, 1502
AluI AGCT 8 cut(s) 290, 331, 341, 356, 560, 595, 1309, 1502
Alw21I GWGCWC 1 cut(s) 384
Alw26I GTCTC 5 cut(s) 458, 460, 1039, 1175, 1181
AlwI GGATC 4 cut(s) 924, 993, 1361, 1374
AlwNI CAGNNNCTG 3 cut(s) 53, 701, 1646
Ama87I CYCGRG 1 cut(s) 1178
AoxI GGCC 2 cut(s) 842, 1222
ApaI GGGCCC 1 cut(s) 846
ApeKI GCWGC 2 cut(s) 1590, 1625
ApoI RAATTY 4 cut(s) 5, 40, 61, 303
Asp700I GAANNNNTTC 1 cut(s) 561
AspLEI GCGC 1 cut(s) 1004
AspS9I GGNCC 3 cut(s) 797, 842, 843
AsuHPI GGTGA 3 cut(s) 265, 1475, 1661
AsuII TTCGAA 1 cut(s) 9
AsuNHI GCTAGC 1 cut(s) 998
AvaI CYCGRG 1 cut(s) 1178
AvaII GGWCC 1 cut(s) 797
BaeGI GKGCMC 1 cut(s) 846
BamHI GGATCC 1 cut(s) 1366
BanII GRGCYC 1 cut(s) 846
Bbv12I GWGCWC 1 cut(s) 384
BbvI GCAGC 2 cut(s) 1602, 1637
BccI CCATC 3 cut(s) 634, 731, 1271
BceAI ACGGC 1 cut(s) 1209
BcgI CGANNNNNNTGC 2 cut(s) 1169, 1203
BciT130I CCWGG 2 cut(s) 719, 892
BciVI GTATCC 2 cut(s) 162, 1024
BclI TGATCA 1 cut(s) 1609
BcoDI GTCTC 5 cut(s) 458, 460, 1039, 1175, 1181
BcuI ACTAGT 2 cut(s) 1034, 1166
BfaI CTAG 6 cut(s) 332, 873, 999, 1035, 1167, 1320
BfmI CTRYAG 1 cut(s) 54
BfuI GTATCC 2 cut(s) 162, 1024
BglII AGATCT 1 cut(s) 671
BisI GCNGC 3 cut(s) 1565, 1591, 1626
BlsI GCNGC 3 cut(s) 1566, 1592, 1627
BmcAI AGTACT 2 cut(s) 25, 1375
Bme1390I CCNGG 2 cut(s) 719, 892
Bme18I GGWCC 1 cut(s) 797
BmeT110I CYCGRG 1 cut(s) 1178
BmgT120I GGNCC 3 cut(s) 797, 842, 843
BmiI GGNNCC 7 cut(s) 798, 843, 844, 889, 1212, 1368, 1383
BmrFI CCNGG 2 cut(s) 719, 892
BmrI ACTGGG 1 cut(s) 1634
BmsI GCATC 4 cut(s) 383, 1013, 1177, 1277
BmtI GCTAGC 1 cut(s) 1002
BmuI ACTGGG 1 cut(s) 1634
BpmI CTGGAG 3 cut(s) 270, 723, 1149
Bpu14I TTCGAA 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 914
BsaBI GATNNNNATC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 1181
BsaJI CCNNGG 3 cut(s) 891, 980, 1253
BsaXI ACNNNNNCTCC 2 cut(s) 1510, 1540
Bsc4I CCNNNNNNNGG 4 cut(s) 822, 1111, 1220, 1646
Bse118I RCCGGY 1 cut(s) 725
Bse1I ACTGG 4 cut(s) 748, 1599, 1640, 1651
Bse3DI GCAATG 1 cut(s) 1545
Bse8I GATNNNNATC 1 cut(s) 150
BseBI CCWGG 2 cut(s) 719, 892
BseDI CCNNGG 3 cut(s) 891, 980, 1253
BseGI GGATG 3 cut(s) 1141, 1401, 1563
BseJI GATNNNNATC 1 cut(s) 150
BseLI CCNNNNNNNGG 4 cut(s) 822, 1111, 1220, 1646
BseMI GCAATG 1 cut(s) 1545
BseMII CTCAG 4 cut(s) 41, 369, 1233, 1681
BseNI ACTGG 4 cut(s) 748, 1599, 1640, 1651
BseSI GKGCMC 1 cut(s) 846
BseXI GCAGC 2 cut(s) 1602, 1637
BseYI CCCAGC 1 cut(s) 608
BsgI GTGCAG 1 cut(s) 714
BshFI GGCC 2 cut(s) 844, 1224
BsiHKAI GWGCWC 1 cut(s) 384
BsiHKCI CYCGRG 1 cut(s) 1178
BsiSI CCGG 1 cut(s) 726
BslFI GGGAC 2 cut(s) 534, 908
BslI CCNNNNNNNGG 4 cut(s) 822, 1111, 1220, 1646
BsmAI GTCTC 5 cut(s) 458, 460, 1039, 1175, 1181
BsmFI GGGAC 2 cut(s) 534, 908
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 2 cut(s) 844, 1224
Bso31I GGTCTC 1 cut(s) 1181
BsoBI CYCGRG 1 cut(s) 1178
Bsp119I TTCGAA 1 cut(s) 9
Bsp120I GGGCCC 1 cut(s) 842
Bsp1286I GDGCHC 2 cut(s) 384, 846
Bsp143I GATC 7 cut(s) 277, 671, 916, 985, 1366, 1444, 1609
Bsp19I CCATGG 1 cut(s) 1253
BspACI CCGC 6 cut(s) 588, 1041, 1516, 1543, 1564, 1686
BspANI GGCC 2 cut(s) 844, 1224
BspCNI CTCAG 4 cut(s) 40, 370, 1232, 1680
BspLI GGNNCC 7 cut(s) 798, 843, 844, 889, 1212, 1368, 1383
BspOI GCTAGC 1 cut(s) 1002
BspPI GGATC 4 cut(s) 924, 993, 1361, 1374
BspT104I TTCGAA 1 cut(s) 9
BspTNI GGTCTC 1 cut(s) 1181
BsrBI CCGCTC 2 cut(s) 1516, 1686
BsrDI GCAATG 1 cut(s) 1545
BsrFI RCCGGY 1 cut(s) 725
BsrI ACTGG 4 cut(s) 748, 1599, 1640, 1651
BssAI RCCGGY 1 cut(s) 725
BssECI CCNNGG 3 cut(s) 891, 980, 1253
BssMI GATC 7 cut(s) 277, 671, 916, 985, 1366, 1444, 1609
BssT1I CCWWGG 2 cut(s) 980, 1253
Bst2UI CCWGG 2 cut(s) 719, 892
Bst4CI ACNGT 8 cut(s) 231, 571, 625, 904, 956, 994, 1142, 1378
BstBI TTCGAA 1 cut(s) 9
BstC8I GCNNGC 2 cut(s) 1000, 1329
BstDEI CTNAG 6 cut(s) 27, 36, 378, 1219, 1313, 1667
BstDSI CCRYGG 1 cut(s) 1253
BstENI CCTNNNNNAGG 1 cut(s) 1218
BstF5I GGATG 3 cut(s) 1141, 1401, 1563
BstHHI GCGC 1 cut(s) 1004
BstKTI GATC 7 cut(s) 280, 674, 919, 988, 1369, 1447, 1612
BstMAI GTCTC 5 cut(s) 458, 460, 1039, 1175, 1181
BstMBI GATC 7 cut(s) 277, 671, 916, 985, 1366, 1444, 1609
BstMWI GCNNNNNNNGC 4 cut(s) 832, 841, 1535, 1570
BstNI CCWGG 2 cut(s) 719, 892
BstNSI RCATGY 2 cut(s) 1156, 1364
BstSCI CCNGG 2 cut(s) 717, 890
BstSFI CTRYAG 1 cut(s) 54
BstSLI GKGCMC 1 cut(s) 846
BstV1I GCAGC 2 cut(s) 1602, 1637
BstX2I RGATCY 3 cut(s) 671, 985, 1366
BstYI RGATCY 3 cut(s) 671, 985, 1366
BsuI GTATCC 2 cut(s) 162, 1024
BsuRI GGCC 2 cut(s) 844, 1224
BtgI CCRYGG 1 cut(s) 1253
BtgZI GCGATG 1 cut(s) 258
BtsCI GGATG 3 cut(s) 1141, 1401, 1563
BtsI GCAGTG 1 cut(s) 768
BtsIMutI CAGTG 5 cut(s) 755, 768, 961, 1434, 1644
Cac8I GCNNGC 2 cut(s) 1000, 1329
CaiI CAGNNNCTG 3 cut(s) 53, 701, 1646
CfoI GCGC 1 cut(s) 1004
Cfr10I RCCGGY 1 cut(s) 725
Cfr13I GGNCC 3 cut(s) 797, 842, 843
Csp6I GTAC 3 cut(s) 24, 732, 1374
CspCI CAANNNNNGTGG 2 cut(s) 1359, 1394
CviQI GTAC 3 cut(s) 24, 732, 1374
DdeI CTNAG 6 cut(s) 27, 36, 378, 1219, 1313, 1667
DpnI GATC 7 cut(s) 279, 673, 918, 987, 1368, 1446, 1611
DpnII GATC 7 cut(s) 277, 671, 916, 985, 1366, 1444, 1609
Eco130I CCWWGG 2 cut(s) 980, 1253
Eco24I GRGCYC 1 cut(s) 846
Eco31I GGTCTC 1 cut(s) 1181
Eco47I GGWCC 1 cut(s) 797
Eco57I CTGAAG 3 cut(s) 129, 576, 1317
Eco88I CYCGRG 1 cut(s) 1178
EcoNI CCTNNNNNAGG 1 cut(s) 1218
EcoO109I RGGNCCY 2 cut(s) 797, 842
EcoRI GAATTC 1 cut(s) 5
EcoRII CCWGG 2 cut(s) 717, 890
EcoT14I CCWWGG 2 cut(s) 980, 1253
EcoT22I ATGCAT 1 cut(s) 1192
EcoT38I GRGCYC 1 cut(s) 846
ErhI CCWWGG 2 cut(s) 980, 1253
FaqI GGGAC 2 cut(s) 534, 908
FauI CCCGC 2 cut(s) 1034, 1679
FbaI TGATCA 1 cut(s) 1609
Fnu4HI GCNGC 3 cut(s) 1565, 1591, 1626
FokI GGATG 3 cut(s) 1148, 1408, 1570
FriOI GRGCYC 1 cut(s) 846
Fsp4HI GCNGC 3 cut(s) 1565, 1591, 1626
FspBI CTAG 6 cut(s) 332, 873, 999, 1035, 1167, 1320
GlaI GCGC 1 cut(s) 1003
GluI GCNGC 3 cut(s) 1565, 1591, 1626
GsaI CCCAGC 1 cut(s) 612
GsuI CTGGAG 3 cut(s) 270, 723, 1149
HaeIII GGCC 2 cut(s) 844, 1224
HapII CCGG 1 cut(s) 726
HhaI GCGC 1 cut(s) 1004
Hin6I GCGC 1 cut(s) 1002
HinP1I GCGC 1 cut(s) 1002
HindIII AAGCTT 2 cut(s) 1307, 1500
HinfI GANTC 7 cut(s) 50, 83, 88, 93, 146, 212, 453
HpaII CCGG 1 cut(s) 726
HphI GGTGA 3 cut(s) 265, 1475, 1661
Hpy166II GTNNAC 2 cut(s) 1432, 1673
Hpy188I TCNGA 8 cut(s) 211, 301, 309, 379, 460, 1015, 1336, 1605
Hpy188III TCNNGA 8 cut(s) 132, 155, 196, 669, 1128, 1178, 1180, 1533
Hpy8I GTNNAC 2 cut(s) 1432, 1673
HpyAV CCTTC 5 cut(s) 388, 469, 641, 1463, 1684
HpyCH4III ACNGT 8 cut(s) 231, 571, 625, 904, 956, 994, 1142, 1378
HpyCH4IV ACGT 3 cut(s) 523, 537, 1675
HpyCH4V TGCA 8 cut(s) 374, 684, 695, 1145, 1190, 1241, 1268, 1625
HpyF10VI GCNNNNNNNGC 4 cut(s) 832, 841, 1535, 1570
HpyF3I CTNAG 6 cut(s) 27, 36, 378, 1219, 1313, 1667
HpySE526I ACGT 3 cut(s) 523, 537, 1675
HspAI GCGC 1 cut(s) 1002
Ksp22I TGATCA 1 cut(s) 1609
Kzo9I GATC 7 cut(s) 277, 671, 916, 985, 1366, 1444, 1609
LmnI GCTCC 2 cut(s) 832, 1578
Lsp1109I GCAGC 2 cut(s) 1602, 1637
LweI GCATC 4 cut(s) 383, 1013, 1177, 1277
MaeI CTAG 6 cut(s) 332, 873, 999, 1035, 1167, 1320
MaeII ACGT 3 cut(s) 523, 537, 1675
MaeIII GTNAC 3 cut(s) 1398, 1642, 1649
MalI GATC 7 cut(s) 279, 673, 918, 987, 1368, 1446, 1611
MbiI CCGCTC 2 cut(s) 1516, 1686
MboI GATC 7 cut(s) 277, 671, 916, 985, 1366, 1444, 1609
MboII GAAGA 7 cut(s) 176, 272, 649, 656, 657, 952, 1645
MflI RGATCY 3 cut(s) 671, 985, 1366
MhlI GDGCHC 2 cut(s) 384, 846
MluCI AATT 9 cut(s) 5, 40, 61, 97, 174, 256, 303, 310, 1293
MlyI GAGTC 4 cut(s) 44, 97, 206, 462
MmeI TCCRAC 4 cut(s) 458, 734, 1527, 1583
Mph1103I ATGCAT 1 cut(s) 1192
MroXI GAANNNNTTC 1 cut(s) 561
MseI TTAA 3 cut(s) 255, 792, 1356
MslI CAYNNNNRTG 2 cut(s) 931, 1155
MspI CCGG 1 cut(s) 726
MspR9I CCNGG 2 cut(s) 719, 892
Mva1269I GAATGC 1 cut(s) 17
MvaI CCWGG 2 cut(s) 719, 892
MwoI GCNNNNNNNGC 4 cut(s) 832, 841, 1535, 1570
NcoI CCATGG 1 cut(s) 1253
NdeII GATC 7 cut(s) 277, 671, 916, 985, 1366, 1444, 1609
NheI GCTAGC 1 cut(s) 998
NlaIV GGNNCC 7 cut(s) 798, 843, 844, 889, 1212, 1368, 1383
NmuCI GTSAC 2 cut(s) 1642, 1649
NsiI ATGCAT 1 cut(s) 1192
NspI RCATGY 2 cut(s) 1156, 1364
NspV TTCGAA 1 cut(s) 9
OliI CACNNNNGTG 1 cut(s) 931
PaeR7I CTCGAG 1 cut(s) 1178
PciI ACATGT 1 cut(s) 1152
PctI GAATGC 1 cut(s) 17
PdmI GAANNNNTTC 1 cut(s) 561
PfeI GAWTC 3 cut(s) 83, 93, 146
PflMI CCANNNNNTGG 1 cut(s) 1646
PkrI GCNGC 3 cut(s) 1566, 1592, 1627
PleI GAGTC 4 cut(s) 44, 96, 206, 461
PpsI GAGTC 4 cut(s) 44, 96, 206, 461
PpuMI RGGWCCY 1 cut(s) 797
PscI ACATGT 1 cut(s) 1152
Psp5II RGGWCCY 1 cut(s) 797
Psp6I CCWGG 2 cut(s) 717, 890
PspFI CCCAGC 1 cut(s) 608
PspGI CCWGG 2 cut(s) 717, 890
PspN4I GGNNCC 7 cut(s) 798, 843, 844, 889, 1212, 1368, 1383
PspOMI GGGCCC 1 cut(s) 842
PspPI GGNCC 3 cut(s) 797, 842, 843
PspPPI RGGWCCY 1 cut(s) 797
PstNI CAGNNNCTG 3 cut(s) 53, 701, 1646
PsuI RGATCY 3 cut(s) 671, 985, 1366
RsaI GTAC 3 cut(s) 25, 733, 1375
RsaNI GTAC 3 cut(s) 24, 732, 1374
RseI CAYNNNNRTG 2 cut(s) 931, 1155
SaqAI TTAA 3 cut(s) 255, 792, 1356
SatI GCNGC 3 cut(s) 1565, 1591, 1626
Sau3AI GATC 7 cut(s) 277, 671, 916, 985, 1366, 1444, 1609
Sau96I GGNCC 3 cut(s) 797, 842, 843
ScaI AGTACT 2 cut(s) 25, 1375
SchI GAGTC 4 cut(s) 44, 97, 206, 462
ScrFI CCNGG 2 cut(s) 719, 892
SduI GDGCHC 2 cut(s) 384, 846
SfaNI GCATC 4 cut(s) 383, 1013, 1177, 1277
SfcI CTRYAG 1 cut(s) 54
Sfr274I CTCGAG 1 cut(s) 1178
SfuI TTCGAA 1 cut(s) 9
SgrAI CRCCGGYG 1 cut(s) 725
SinI GGWCC 1 cut(s) 797
SlaI CTCGAG 1 cut(s) 1178
SmiMI CAYNNNNRTG 2 cut(s) 931, 1155
SmlI CTYRAG 2 cut(s) 929, 1178
SmoI CTYRAG 2 cut(s) 929, 1178
SpeI ACTAGT 2 cut(s) 1034, 1166
Sse9I AATT 9 cut(s) 5, 40, 61, 97, 174, 256, 303, 310, 1293
SsiI CCGC 6 cut(s) 588, 1041, 1516, 1543, 1564, 1686
SspMI CTAG 6 cut(s) 332, 873, 999, 1035, 1167, 1320
StyD4I CCNGG 2 cut(s) 717, 890
StyI CCWWGG 2 cut(s) 980, 1253
TaaI ACNGT 8 cut(s) 231, 571, 625, 904, 956, 994, 1142, 1378
TaiI ACGT 3 cut(s) 526, 540, 1678
TaqI TCGA 5 cut(s) 9, 70, 86, 91, 1179
TasI AATT 9 cut(s) 5, 40, 61, 97, 174, 256, 303, 310, 1293
TatI WGTACW 2 cut(s) 23, 1373
TauI GCSGC 1 cut(s) 1567
TfiI GAWTC 3 cut(s) 83, 93, 146
Tru1I TTAA 3 cut(s) 255, 792, 1356
Tru9I TTAA 3 cut(s) 255, 792, 1356
TscAI CASTG 5 cut(s) 755, 768, 961, 1434, 1651
TseFI GTSAC 2 cut(s) 1642, 1649
TseI GCWGC 2 cut(s) 1590, 1625
Tsp45I GTSAC 2 cut(s) 1642, 1649
TspDTI ATGAA 4 cut(s) 333, 1052, 1173, 1338
TspRI CASTG 5 cut(s) 755, 768, 961, 1434, 1651
Van91I CCANNNNNTGG 1 cut(s) 1646
VpaK11BI GGWCC 1 cut(s) 797
XagI CCTNNNNNAGG 1 cut(s) 1218
XapI RAATTY 4 cut(s) 5, 40, 61, 303
XceI RCATGY 2 cut(s) 1156, 1364
XhoI CTCGAG 1 cut(s) 1178
XmnI GAANNNNTTC 1 cut(s) 561
XspI CTAG 6 cut(s) 332, 873, 999, 1035, 1167, 1320
ZrmI AGTACT 2 cut(s) 25, 1375
Zsp2I ATGCAT 1 cut(s) 1192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.