FvH4_3g28110

chromodomain-helicase-DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007957
Physical Location & Seq
Reverse (-)
21097293 .. 21115206
17914 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g28110.t4

Sequence Viewer

Length: 5241 bp
ATGGCGTTTTTCAGGAACTACTCTAGTGAAACAGTTTCGCAAAGTTTCCTGGATGACCAGAGCCAGCGACCACAGGATGACAGAACTCACAGGTCCTCAGGGAATGTGGATGCACATGTAATGTCTTATGATAAGGAATTTGATATGAATTTGGATGTGAAGTATCAAAGTGAAGACGAACAAGATGGTCCTAGTGGACTGCAAAATGAGGCAGCTGCTGATGAGGGTACTGGTCCAGGAGTGTCCAATTCCAAGTCATCTGGGAGGAGGACGAATGTGGCTGGAAAATGGGGTTCAACCTTTTGGAAGGATTGTCAACCTATGTGCCCACAGGGTGGATCTGATTCAGGACAGGACACTAAAAGTGGGTCTGAGTACAGAAACGCTGTTGGATCTGAAGATAATTCTTCAGATGTCAGGGAAGATAGATTAGATTCTGAAGATGATGCCGGACCTAAAGTACGAAAGGGACAGCGGGGACATTCTGATATACCTGCAGATGAAATGTTGTCTGATGAATATTATGAGCAGGATGGGGAAGAACAGAGTGACTCGATGCATTATAGAGGGTTTCATCATTCAGTTGGATCCACTTCCAGACTACAAGCAAAGCCAGCCCCTATCACCAATCATAGGACTTCTAGACTTTCAAATAATAATGGAAATTATGTTGACGGCGAAGATGACAATGAAAATATTGATGGTGCTGATGCTGATTACGAAGAAGAGGATGAAGATGAAGATGATCCTGATGATGCAGATTTTGAACCTGATTATGGTGTTGCGAGTGGTCAAGCTGCAAAGAAGGATAAAGATTGGAATGATCAAGATTCAGATGAAGATAATGACAGTGATGATGAACTTGACGTCTCAGATGACGATAATTCTTTTTATGCGAAGAATTCAAAGGGTAGGCATCGTAGTAAAGGTGCACATACTGTAAAACTTAAAAGAGAACGCAAATCATACATTGGATCTAGTCGACAAAGGAGAAGAAAATCATCATTTGATGATGATGAGTCCTCAGCAGACGACTCTGAGAGTGACAGTGATGAGGGTTTCAAAAGCACAAGAAGAAAAGGTGCACATATCCGCAAAAGTAATGGGCGGACAACGAATGTCTCTGGGCGAAATGGTGAAGTACGAGCTTCCACTAGGTCTGTTCGGAAGGTATCATATGTTGAAAGTGAAGGAAGTGATGAAGCTGATGAAGGCAAGAAAAAGAAGTCGCAAAAGGAGGATATTGAGGAGGAGGATGGTGACTATATTGAAAAGGTGCTCTGGCATCAACCAAAGGGCATGGCTGAAGATGCTATCAGGAATAATAGATCCGCAGAGCCTCTGCTGTTAAGTCACTTGTTTGACTCTGAGCCAGATTGGTGTAATACAGAATTTTTGATCAAATGGAAGGGCTTATCACATTTGCACTGCCAGTGGAAAATATTTTCTGAGCTACAAAGTCTTAGCGGTTTCAAGAAGGTTGTAAACTATACTAAAAAGGTGACGGAAGATGCCAGGTACAGGAAGACCATCTCACGTGAAGAGATTGAGGTCCATGACGTGAGCAAGGAAATGGACTTAGATCTTATCAAGCAAAACAGTCAGGTGGAGAGAATAATTGCAGACCGCATCAAGCAAGATAGCTCAGGTGATGTAGTACCAGAGTATCTTGTCAAGTGGCAGGGATTGTCTTATGCTGAAGCGACATGGGAGAAGGATGTCGATATTGCATTTGCACAAGATGCTATTGATGAGTTCAAGGCTCGTGAGGCTGCAATGGCTGTACAAGGGAAAATGGTGGATCTCCAACGAAAGAAAAGCAAAGGAAGTCTAAGGAAGCTGGATGAACAGCCTGAATGGTTGAAAGGAGGAAAGCTTCGTGATTATCAGCTTGAGGGTTTGAATTTTCTTGTTAATAGTTGGAGAAATGATACCAACGTCATTTTAGCTGATGAAATGGGTCTTGGAAAAACTGTTCAATCAGTTTCGATGCTTGGTTTTTTACAGAACGCTCAACAGATCCATGGGCCATTTCTTGTTGTTGTACCATTATCTACTCTGTCAAATTGGGCAAAAGAGTTTAGGAAATGGCTCCCTGATATGAATGTTATAGTGTATGTTGGTACCCGTGCAAGCCGAGAGGTTTGTCAGCAGTATGAATTCCATAACGAGAAAATAATTGGCCGGCCTATAAAATTTAATGCACTGTTGACAACGTATGAAGTGGTTTTAAAGGACAAGGCTGTTCTTTCCAAGATCAAGTGGAATTATCTGATGGTTGATGAAGCTCATAGGCTAAAGAACAGTGAGGCTCAGCTGTACACCACTCTCTTGGAATTTAGCACAAAAAACAAGTTGCTTATAACCGGAACTCCATTGCAGAACAGTGTGGAAGAGTTATGGGCCCTGCTTCACTTCCTCGACCCCCACAAGTTCAATAACAAAGATGAATTTGTTCAGAACTACAAGAATCTCAGTTCGTTTAATGAGATTGAGCTTGCTAATCTTCACATGGAATTGAGGCCTCACATTCTCCGAAGAGTTATTAAGGACGTTGAAAAGTCTTTACCTCCAAAAATTGAACGAATTCTTAGGGTTGAAATGTCTCCTCTTCAGAAACAGTATTACAAGTGGATTTTGGAGCGCAACTTCCATAACTTGAACAAAGGAGTTCGTGGGAATCAGGTTTCATTGTTGAATATTGTAGTAGAGTTGAAGAAATGCTGTAATCATCCCTTCCTTTTTGAAAGTGCTGACCATGGCTATGGCGGAGACTCAAGCAGCAAAGATGGCAGTAAATTGGAGAGAATTATTTTAAGCAGTGGGAAGTTGGTTATACTTGATAAGCTTCTTGTTAGATTACATGAGACGAAGCACCGTGTTCTCATCTTTTCCCAGATGGTAAGAATGTTGGACATACTAGCTGAGTATATGTCGCATAGAGGTTTTCAATTTCAGCGACTTGATGGAAGTACGAAGGCTGATTTACGGCACCAGGCTATGGAACATTTCAATGCACCTGGTAGTGAAGATTTTTGCTTCCTTCTTTCAACACGAGCAGGTGGCTTAGGAATCAACCTTGCCACGGCGGACACGGTTATAATTTTTGATTCAGATTGGAATCCTCAAAATGACTTGCAGGCAATGAGTAGGGCTCACAGAATTGGGCAACAGGAAGTGGTCAACATCTATAGATTTGTAACAAGCAAGAGTGTTGAGGAAGATATCCTGGAGAGAGCTAAGAAAAAGATGGTGCTTGACCATTTGGTGATTCAAAAGTTAAATGCAGAGGGAAGATTGGAGAAGAAAGAAACCAAAAAGGGAAGTTTGTTTGATAAAAACGAGCTCTCTGCTATCTTAAGATTTGGGGCTGAGGAACTGTTTAAGGAAGAGAAAAATGAAGAAGAAAGCAAGAAAAGACTATTGAGTATGGATATTGATGAGATTCTTGAGAGGGCAGAGAAGGTTGAAGAGAAGGAAACTACGGAGGATGGACATGAATTGCTGAGTGCTTTTAAGGTTGCAAATTTTGGTTCTGCCGAAGATGATGGAAGTTTTTGGAGTCGTTGGATAAAGCCAGATGCAGTATCCCAAGCCGAGGAAGCTCTTGCTCCTCGCGCTACGAGGAATACCAAGAGCTATGCAGAAGCTGCTCAGCCTGATAGGAGTAATAAAAGGAAAAAAAAAGAATCTGAGCCTCAGGAAAGAGTCCAGAAAAGGCGCAAACCAGATCATTCAGTTCCATCAGCACCAATGATCGACGGAGCGTCTGCTCAAGTAAGAGGATGGTCATTTGGAAATGTGTCTAAACGAGATGCATTGCGGTTTTCCCGTGCGGTTATGAAGTTTGGAAATGAGAGCCAAATTGGCTTGATTGTTGAGGAGGTCGGTGGTGCAATTGCAGCAGCTTCACCTGAAGCCCAGGTTGAGCTTTTCAATGCCTTGATTGATGGTTGCAGGGAAGCAGTGGAAGTGGGGAGTTTGGACCAGAAGGGACCCTTGCTTGATTTCTTTGGTGTCCCTGTGAAGGCTAGTGATCTGGTCAACCGTGTTCAAGAACTTCAGCTCCTAGCGAAGCGCATTATACGATATGAAGATCCCATTGGCCAGTTCCGTGTGCTGATGTACCTCAAACCTTCAAACTGGTCAAAAGGTTGTGGCTGGAATCAGATTGACGATGCTAGGTTACTTCTCGGAATATACTACCATGGGTTTGGCAATTGGGAAAAGATACGATTAGATGAAAGGCTTGGCCTTATGAAGAAGATTGCTCCTGTAGAACTTCAGCACCATGAAACTTTCTTACCCCGTGCGCCAAATTTGAGAGATCGTGCTAATGCTCTTTTGGAAATGGAACTTGCAGCTCTTGGGGGAAAGAATGCAAATGCTAAAGTTGGTCGTAAAGCTTCCAAAGAGAGGGAAAATCCTGTCCCTGTTCCTGTGTCTCGCACTGGGGTCAAGAAAGGAAAAGTAGGTCCTTCTAGAGCTAATGTCCAAATGATCAAGGACAAACCTCTGAAACCCCAGAGAGTTGAACCTTTAGTAAAAGAAGAGGGTGAAATGTCTGATGATGAAGAAGTATATGAGAAGTTTAAGGAAGAGAAATGGATGGAATGGTGTGAAGAGATGATGGCTAGTGAAATTAAAACTCTCAACCGTCTCCATCGGTTGCAGACGACTAGCGCCAATCTTCCAAAGGAGAAGGTGCTTTCAAAAATTCGCAATTATTTACAGCTTCTTGGACGAAGGATAGATCAAATTGTTCTGGACAATGAGGAGGAACCATATGGACAGGATAGGATGACCACGCGTTTGTGGAACTTTGTTTCTACGTTTTCGAACCTATCTGGAGAGCGACTTCACCAAATATATTCGAAACTTAAACAGGAGCAGGATGAGGAAGCTGGTCCTTCCCACATCAATGGCTCTGCATCCGGCCCTTTTGGCAGAGATAGTGACCCTACTTCCTTCTCCCATCTTTCTGAGAGGCAAAGAGGGTATAAAAGTATTAATAACCAAACATTTGAACCTCTGAAGGGTTTCGATACAGCTAAATTTGAAGCATGGAAACGAAGGAGAAGAGGGGAGACCGATAGTCCATCTCAAAGACCTTTAATTAATGGCTCTCGGCCCACTGATCCAAACTCGGTGGGGATACTTGGTGCCGGACCATCTGAAAACAGACGCTCTCTTAATGAAAAGCATTATAAGACTCGTCAAACCGGTGTACCACCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chromo DUF4208 Helicase_C SLIDE SNF2_N

Protein Analysis

1747

Amino Acids

198.63

Weight (kDa)

5.85

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chromo PF00385 535 - 589 1.3e-14 Chromo (CHRromatin Organisation MOdifier) domain
ResIII PF04851 625 - 792 4.7e-11 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 629 - 913 6.3e-86 SNF2-related domain
DEAD PF00270 640 - 797 2.6e-07 DEAD/DEAH box helicase
HDA2-3 PF11496 864 - 1088 2.6e-11 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 943 - 1056 2.6e-26 Helicase conserved C-terminal domain
HTH_CHD1_Hrp3 PF23588 1368 - 1449 7.4e-23 ATP-dependent helicase CHD1-2/hrp3 HTH domain
CHD1-like_C PF13907 1532 - 1608 5.8e-07 Chromodomain-helicase-DNA-binding protein 1-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2363, 3101, 5208
AarI CACCTGC 1 cut(s) 3050
AatII GACGTC 1 cut(s) 870
Acc36I ACCTGC 2 cut(s) 502, 3050
Acc65I GGTACC 1 cut(s) 2123
AccB1I GGYRCC 3 cut(s) 2123, 2991, 5162
AccB7I CCANNNNNTGG 2 cut(s) 335, 3001
AccI GTMKAC 1 cut(s) 982
AccII CGCG 2 cut(s) 3618, 4810
AcoI YGGCCR 2 cut(s) 2182, 4105
AcvI CACGTG 1 cut(s) 1538
AcyI GRCGYC 1 cut(s) 867
AdeI CACNNNGTG 1 cut(s) 335
AflII CTTAAG 1 cut(s) 3358
AflIII ACRYGT 2 cut(s) 115, 4808
AgeI ACCGGT 1 cut(s) 5222
AhdI GACNNNNNGTC 2 cut(s) 3766, 5094
AjiI CACGTC 1 cut(s) 1561
AjnI CCWGG 7 cut(s) 48, 235, 1514, 2994, 3019, 3228, 3921
AjuI GAANNNNNNNTTGG 4 cut(s) 1947, 1979, 4086, 4118
AloI GAACNNNNNNTCC 4 cut(s) 1959, 1991, 4050, 4082
Alw21I GWGCWC 4 cut(s) 934, 1087, 1281, 3348
Alw26I GTCTC 8 cut(s) 874, 1126, 2610, 2766, 2861, 4449, 4664, 5081
Alw44I GTGCAC 2 cut(s) 930, 1083
AlwNI CAGNNNCTG 2 cut(s) 218, 3650
AoxI GGCC 9 cut(s) 2027, 2182, 2186, 2403, 2522, 4105, 4252, 4936, 5129
ApaI GGGCCC 1 cut(s) 2407
ApaLI GTGCAC 2 cut(s) 930, 1083
ApeKI GCWGC 9 cut(s) 212, 215, 797, 1772, 2781, 3650, 3902, 3905, 4361
ArsI GACNNNNNNTTYG 2 cut(s) 4795, 4827
AseI ATTAAT 2 cut(s) 5010, 5118
AsiGI ACCGGT 1 cut(s) 5222
Asp700I GAANNNNTTC 3 cut(s) 2454, 2586, 5039
Asp718I GGTACC 1 cut(s) 2123
AspLEI GCGC 6 cut(s) 2646, 3620, 3723, 4080, 4315, 4685
AsuHPI GGTGA 9 cut(s) 616, 1148, 1271, 1513, 1661, 3280, 3903, 4568, 4853
AsuII TTCGAA 2 cut(s) 4838, 4874
AxyI CCTNAGG 2 cut(s) 97, 3699
BaeGI GKGCMC 4 cut(s) 329, 934, 1087, 2407
BaeI ACNNNNGTAYC 5 cut(s) 1649, 1682, 1923, 1956, 5211
BalI TGGCCA 1 cut(s) 4107
BamHI GGATCC 1 cut(s) 587
BanI GGYRCC 3 cut(s) 2123, 2991, 5162
BanII GRGCYC 3 cut(s) 2407, 3157, 3348
BauI CACGAG 2 cut(s) 1764, 3054
BbrPI CACGTG 1 cut(s) 1538
BbsI GAAGAC 2 cut(s) 180, 1532
Bbv12I GWGCWC 4 cut(s) 934, 1087, 1281, 3348
BbvCI CCTCAGC 2 cut(s) 1024, 3372
BbvI GCAGC 9 cut(s) 202, 224, 784, 1759, 2793, 3637, 3914, 3917, 4373
BceAI ACGGC 3 cut(s) 691, 3005, 3102
BcgI CGANNNNNNTGC 2 cut(s) 3332, 3366
BciT130I CCWGG 7 cut(s) 50, 237, 1516, 2996, 3021, 3230, 3923
BciVI GTATCC 2 cut(s) 3598, 5148
BclI TGATCA 3 cut(s) 823, 1398, 4500
BcoDI GTCTC 8 cut(s) 874, 1126, 2610, 2766, 2861, 4449, 4664, 5081
BfmI CTRYAG 3 cut(s) 495, 3190, 4275
BfoI RGCGCY 1 cut(s) 4686
BfrI CTTAAG 1 cut(s) 3358
BfuAI ACCTGC 2 cut(s) 502, 3050
BfuI GTATCC 2 cut(s) 3598, 5148
BglI GCCNNNNNGGC 2 cut(s) 3867, 4944
BglII AGATCT 1 cut(s) 1582
BisI GCNGC 9 cut(s) 213, 216, 798, 1773, 2782, 3651, 3903, 3906, 4362
BlpI GCTNAGC 2 cut(s) 2313, 3654
BlsI GCNGC 9 cut(s) 214, 217, 799, 1774, 2783, 3652, 3904, 3907, 4363
Bme1390I CCNGG 7 cut(s) 50, 237, 1516, 2996, 3021, 3230, 3923
BmeRI GACNNNNNGTC 2 cut(s) 3766, 5094
BmgBI CACGTC 1 cut(s) 1561
BmiI GGNNCC 9 cut(s) 589, 2093, 2125, 2405, 2993, 3996, 3997, 4782, 5164
BmrFI CCNGG 7 cut(s) 50, 237, 1516, 2996, 3021, 3230, 3923
BmrI ACTGGG 1 cut(s) 4461
BmuI ACTGGG 1 cut(s) 4461
BpiI GAAGAC 2 cut(s) 180, 1532
BplI GAGNNNNNCTC 2 cut(s) 3139, 3171
BpmI CTGGAG 2 cut(s) 3251, 4869
Bpu10I CCTNAGC 4 cut(s) 1024, 1645, 3067, 3372
Bpu1102I GCTNAGC 2 cut(s) 2313, 3654
Bpu14I TTCGAA 2 cut(s) 4838, 4874
BpuEI CTTGAG 4 cut(s) 1913, 2761, 3470, 3759
BsaAI YACGTR 1 cut(s) 1538
BsaBI GATNNNNATC 1 cut(s) 3120
BsaHI GRCGYC 1 cut(s) 867
BsaI GGTCTC 1 cut(s) 5081
BsaJI CCNNGG 6 cut(s) 2023, 2758, 3084, 3597, 3921, 4207
BsaWI WCCGGW 2 cut(s) 2366, 5222
BsaXI ACNNNNNCTCC 4 cut(s) 2356, 2386, 4050, 4080
Bse118I RCCGGY 2 cut(s) 2184, 5222
Bse1I ACTGG 5 cut(s) 235, 1432, 4108, 4148, 4456
Bse21I CCTNAGG 2 cut(s) 97, 3699
Bse3DI GCAATG 4 cut(s) 1782, 2375, 3150, 3818
Bse8I GATNNNNATC 1 cut(s) 3120
BseBI CCWGG 7 cut(s) 50, 237, 1516, 2996, 3021, 3230, 3923
BseDI CCNNGG 6 cut(s) 2023, 2758, 3084, 3597, 3921, 4207
BseJI GATNNNNATC 1 cut(s) 3120
BseMI GCAATG 4 cut(s) 1782, 2375, 3150, 3818
BseNI ACTGG 5 cut(s) 235, 1432, 4108, 4148, 4456
BseRI GAGGAG 7 cut(s) 280, 1262, 1265, 2598, 3603, 3896, 4790
BseSI GKGCMC 4 cut(s) 329, 934, 1087, 2407
BseXI GCAGC 9 cut(s) 202, 224, 784, 1759, 2793, 3637, 3914, 3917, 4373
Bsh1236I CGCG 2 cut(s) 3618, 4810
BshFI GGCC 9 cut(s) 2029, 2184, 2188, 2405, 2524, 4107, 4254, 4938, 5131
BshNI GGYRCC 3 cut(s) 2123, 2991, 5162
BshTI ACCGGT 1 cut(s) 5222
BsiHKAI GWGCWC 4 cut(s) 934, 1087, 1281, 3348
BsiSI CCGG 6 cut(s) 450, 2185, 2367, 4935, 5166, 5223
BslFI GGGAC 5 cut(s) 483, 492, 4004, 4008, 4415
BsmAI GTCTC 8 cut(s) 874, 1126, 2610, 2766, 2861, 4449, 4664, 5081
BsmBI CGTCTC 3 cut(s) 874, 2861, 4664
BsmFI GGGAC 5 cut(s) 483, 492, 4004, 4008, 4415
BsmI GAATGC 1 cut(s) 4384
BsnI GGCC 9 cut(s) 2029, 2184, 2188, 2405, 2524, 4107, 4254, 4938, 5131
Bso31I GGTCTC 1 cut(s) 5081
Bsp119I TTCGAA 2 cut(s) 4838, 4874
Bsp120I GGGCCC 1 cut(s) 2403
Bsp1286I GDGCHC 7 cut(s) 329, 934, 1087, 1281, 2407, 3157, 3348
Bsp1407I TGTACA 2 cut(s) 1783, 2319
Bsp1720I GCTNAGC 2 cut(s) 2313, 3654
Bsp19I CCATGG 3 cut(s) 2023, 2758, 4207
BspANI GGCC 9 cut(s) 2029, 2184, 2188, 2405, 2524, 4107, 4254, 4938, 5131
BspFNI CGCG 2 cut(s) 3618, 4810
BspLI GGNNCC 9 cut(s) 589, 2093, 2125, 2405, 2993, 3996, 3997, 4782, 5164
BspMAI CTGCAG 1 cut(s) 499
BspMI ACCTGC 2 cut(s) 502, 3050
BspT104I TTCGAA 2 cut(s) 4838, 4874
BspT107I GGYRCC 3 cut(s) 2123, 2991, 5162
BspTI CTTAAG 1 cut(s) 3358
BspTNI GGTCTC 1 cut(s) 5081
BsrDI GCAATG 4 cut(s) 1782, 2375, 3150, 3818
BsrFI RCCGGY 2 cut(s) 2184, 5222
BsrGI TGTACA 2 cut(s) 1783, 2319
BsrI ACTGG 5 cut(s) 235, 1432, 4108, 4148, 4456
BssAI RCCGGY 2 cut(s) 2184, 5222
BssECI CCNNGG 6 cut(s) 2023, 2758, 3084, 3597, 3921, 4207
BssNI GRCGYC 1 cut(s) 867
BssSI CACGAG 2 cut(s) 1764, 3054
BssT1I CCWWGG 3 cut(s) 2023, 2758, 4207
Bst2BI CACGAG 2 cut(s) 1764, 3054
Bst2UI CCWGG 7 cut(s) 50, 237, 1516, 2996, 3021, 3230, 3923
BstACI GRCGYC 1 cut(s) 867
BstAFI CTTAAG 1 cut(s) 3358
BstAPI GCANNNNNTGC 2 cut(s) 1742, 3650
BstAUI TGTACA 2 cut(s) 1783, 2319
BstBAI YACGTR 1 cut(s) 1538
BstBI TTCGAA 2 cut(s) 4838, 4874
BstC8I GCNNGC 6 cut(s) 65, 615, 2134, 2186, 2499, 3141
BstDSI CCRYGG 4 cut(s) 2023, 2758, 3084, 4207
BstFNI CGCG 2 cut(s) 3618, 4810
BstH2I RGCGCY 1 cut(s) 4686
BstHHI GCGC 6 cut(s) 2646, 3620, 3723, 4080, 4315, 4685
BstMAI GTCTC 8 cut(s) 874, 1126, 2610, 2766, 2861, 4449, 4664, 5081
BstNI CCWGG 7 cut(s) 50, 237, 1516, 2996, 3021, 3230, 3923
BstNSI RCATGY 1 cut(s) 119
BstSCI CCNGG 7 cut(s) 48, 235, 1514, 2994, 3019, 3228, 3921
BstSFI CTRYAG 3 cut(s) 495, 3190, 4275
BstSLI GKGCMC 4 cut(s) 329, 934, 1087, 2407
BstUI CGCG 2 cut(s) 3618, 4810
BstV1I GCAGC 9 cut(s) 202, 224, 784, 1759, 2793, 3637, 3914, 3917, 4373
BstV2I GAAGAC 2 cut(s) 180, 1532
BstX2I RGATCY 9 cut(s) 338, 392, 587, 974, 1328, 1582, 1801, 2019, 4096
BstXI CCANNNNNNTGG 4 cut(s) 2332, 2765, 4214, 4922
BstYI RGATCY 9 cut(s) 338, 392, 587, 974, 1328, 1582, 1801, 2019, 4096
Bsu36I CCTNAGG 2 cut(s) 97, 3699
BsuI GTATCC 2 cut(s) 3598, 5148
BsuRI GGCC 9 cut(s) 2029, 2184, 2188, 2405, 2524, 4107, 4254, 4938, 5131
BtgI CCRYGG 4 cut(s) 2023, 2758, 3084, 4207
BtrI CACGTC 1 cut(s) 1561
BtsI GCAGTG 3 cut(s) 1426, 2827, 3972
BveI ACCTGC 2 cut(s) 502, 3050
Cac8I GCNNGC 6 cut(s) 65, 615, 2134, 2186, 2499, 3141
CaiI CAGNNNCTG 2 cut(s) 218, 3650
CfoI GCGC 6 cut(s) 2646, 3620, 3723, 4080, 4315, 4685
Cfr10I RCCGGY 2 cut(s) 2184, 5222
CseI GACGC 2 cut(s) 3756, 5193
CsiI ACCWGGT 1 cut(s) 3019
CspAI ACCGGT 1 cut(s) 5222
CspCI CAANNNNNGTGG 2 cut(s) 5130, 5165
DraI TTTAAA 1 cut(s) 2232
DraIII CACNNNGTG 1 cut(s) 335
DriI GACNNNNNGTC 2 cut(s) 3766, 5094
EaeI YGGCCR 2 cut(s) 2182, 4105
Eam1105I GACNNNNNGTC 2 cut(s) 3766, 5094
EciI GGCGGA 3 cut(s) 1123, 2784, 3104
Ecl136II GAGCTC 1 cut(s) 3346
Eco130I CCWWGG 3 cut(s) 2023, 2758, 4207
Eco147I AGGCCT 1 cut(s) 2524
Eco24I GRGCYC 3 cut(s) 2407, 3157, 3348
Eco31I GGTCTC 1 cut(s) 5081
Eco32I GATATC 1 cut(s) 3226
Eco53kI GAGCTC 1 cut(s) 3346
Eco72I CACGTG 1 cut(s) 1538
Eco81I CCTNAGG 2 cut(s) 97, 3699
EcoICRI GAGCTC 1 cut(s) 3346
EcoO109I RGGNCCY 4 cut(s) 93, 2404, 3995, 4475
EcoRI GAATTC 3 cut(s) 901, 2159, 2586
EcoRII CCWGG 7 cut(s) 48, 235, 1514, 2994, 3019, 3228, 3921
EcoRV GATATC 1 cut(s) 3226
EcoT14I CCWWGG 3 cut(s) 2023, 2758, 4207
EcoT22I ATGCAT 2 cut(s) 561, 3820
EcoT38I GRGCYC 3 cut(s) 2407, 3157, 3348
ErhI CCWWGG 3 cut(s) 2023, 2758, 4207
Esp3I CGTCTC 3 cut(s) 874, 2861, 4664
FalI AAGNNNNNCTT 2 cut(s) 3983, 4015
FaqI GGGAC 5 cut(s) 483, 492, 4004, 4008, 4415
FauI CCCGC 1 cut(s) 468
FauNDI CATATG 2 cut(s) 1177, 4786
FbaI TGATCA 3 cut(s) 823, 1398, 4500
FblI GTMKAC 1 cut(s) 982
Fnu4HI GCNGC 9 cut(s) 213, 216, 798, 1773, 2782, 3651, 3903, 3906, 4362
FriOI GRGCYC 3 cut(s) 2407, 3157, 3348
FseI GGCCGGCC 1 cut(s) 2188
Fsp4HI GCNGC 9 cut(s) 213, 216, 798, 1773, 2782, 3651, 3903, 3906, 4362
GlaI GCGC 6 cut(s) 2645, 3619, 3722, 4079, 4314, 4684
GluI GCNGC 9 cut(s) 213, 216, 798, 1773, 2782, 3651, 3903, 3906, 4362
GsuI CTGGAG 2 cut(s) 3251, 4869
HaeII RGCGCY 1 cut(s) 4686
HaeIII GGCC 9 cut(s) 2029, 2184, 2188, 2405, 2524, 4107, 4254, 4938, 5131
HapII CCGG 6 cut(s) 450, 2185, 2367, 4935, 5166, 5223
HgaI GACGC 2 cut(s) 3756, 5193
HhaI GCGC 6 cut(s) 2646, 3620, 3723, 4080, 4315, 4685
Hin1I GRCGYC 1 cut(s) 867
Hin6I GCGC 6 cut(s) 2644, 3618, 3721, 4078, 4313, 4683
HinP1I GCGC 6 cut(s) 2644, 3618, 3721, 4078, 4313, 4683
HincII GTYRAC 6 cut(s) 317, 673, 983, 2211, 3184, 4045
HindII GTYRAC 6 cut(s) 317, 673, 983, 2211, 3184, 4045
HindIII AAGCTT 3 cut(s) 1874, 2846, 4404
HpaII CCGG 6 cut(s) 450, 2185, 2367, 4935, 5166, 5223
HphI GGTGA 9 cut(s) 616, 1148, 1271, 1513, 1661, 3280, 3903, 4568, 4853
Hpy99I CGWCG 1 cut(s) 3764
HpyCH4IV ACGT 7 cut(s) 867, 1537, 1560, 1938, 2216, 2553, 4832
HpySE526I ACGT 7 cut(s) 867, 1537, 1560, 1938, 2216, 2553, 4832
Hsp92I GRCGYC 1 cut(s) 867
HspAI GCGC 6 cut(s) 2644, 3618, 3721, 4078, 4313, 4683
KflI GGGWCCC 1 cut(s) 3995
KpnI GGTACC 1 cut(s) 2127
KroI GCCGGC 1 cut(s) 2184
KroNI GCCGGC 1 cut(s) 2186
Ksp22I TGATCA 3 cut(s) 823, 1398, 4500
LmnI GCTCC 7 cut(s) 2097, 2641, 3616, 3764, 4071, 4276, 4888
Lsp1109I GCAGC 9 cut(s) 202, 224, 784, 1759, 2793, 3637, 3914, 3917, 4373
MabI ACCWGGT 1 cut(s) 3019
MaeII ACGT 7 cut(s) 867, 1537, 1560, 1938, 2216, 2553, 4832
MaeIII GTNAC 8 cut(s) 548, 1043, 1259, 1352, 1501, 3199, 4185, 4955
MfeI CAATTG 2 cut(s) 3897, 4219
MflI RGATCY 9 cut(s) 338, 392, 587, 974, 1328, 1582, 1801, 2019, 4096
MhlI GDGCHC 7 cut(s) 329, 934, 1087, 1281, 2407, 3157, 3348
MlsI TGGCCA 1 cut(s) 4107
MluI ACGCGT 1 cut(s) 4808
MluNI TGGCCA 1 cut(s) 4107
MlyI GAGTC 8 cut(s) 545, 1028, 1028, 1358, 2768, 3571, 3717, 5206
MmeI TCCRAC 6 cut(s) 370, 565, 1831, 1901, 2892, 3548
Mox20I TGGCCA 1 cut(s) 4107
Mph1103I ATGCAT 2 cut(s) 561, 3820
MroNI GCCGGC 1 cut(s) 2184
MroXI GAANNNNTTC 3 cut(s) 2454, 2586, 5039
MscI TGGCCA 1 cut(s) 4107
MslI CAYNNNNRTG 3 cut(s) 2763, 3012, 4920
Msp20I TGGCCA 1 cut(s) 4107
MspA1I CMGCKG 3 cut(s) 215, 475, 2317
MspCI CTTAAG 1 cut(s) 3358
MspI CCGG 6 cut(s) 450, 2185, 2367, 4935, 5166, 5223
MspR9I CCNGG 7 cut(s) 50, 237, 1516, 2996, 3021, 3230, 3923
MunI CAATTG 2 cut(s) 3897, 4219
Mva1269I GAATGC 1 cut(s) 4384
MvaI CCWGG 7 cut(s) 50, 237, 1516, 2996, 3021, 3230, 3923
MvnI CGCG 2 cut(s) 3618, 4810
NaeI GCCGGC 1 cut(s) 2186
NcoI CCATGG 3 cut(s) 2023, 2758, 4207
NdeI CATATG 2 cut(s) 1177, 4786
NgoMIV GCCGGC 1 cut(s) 2184
NlaIV GGNNCC 9 cut(s) 589, 2093, 2125, 2405, 2993, 3996, 3997, 4782, 5164
NmeAIII GCCGAG 3 cut(s) 2162, 3622, 5107
NmuCI GTSAC 6 cut(s) 548, 1043, 1259, 1352, 1501, 4955
NsiI ATGCAT 2 cut(s) 561, 3820
NspI RCATGY 1 cut(s) 119
NspV TTCGAA 2 cut(s) 4838, 4874
PacI TTAATTAA 1 cut(s) 5118
PaqCI CACCTGC 1 cut(s) 3050
PceI AGGCCT 1 cut(s) 2524
PciI ACATGT 1 cut(s) 115
PctI GAATGC 1 cut(s) 4384
PdiI GCCGGC 1 cut(s) 2186
PdmI GAANNNNTTC 3 cut(s) 2454, 2586, 5039
PflMI CCANNNNNTGG 2 cut(s) 335, 3001
PfoI TCCNGGA 3 cut(s) 48, 235, 3228
PinAI ACCGGT 1 cut(s) 5222
PkrI GCNGC 9 cut(s) 214, 217, 799, 1774, 2783, 3652, 3904, 3907, 4363
PleI GAGTC 8 cut(s) 545, 1027, 1028, 1358, 2768, 3570, 3716, 5206
PmaCI CACGTG 1 cut(s) 1538
PmlI CACGTG 1 cut(s) 1538
PpsI GAGTC 8 cut(s) 545, 1027, 1028, 1358, 2768, 3570, 3716, 5206
Ppu21I YACGTR 1 cut(s) 1538
PpuMI RGGWCCY 3 cut(s) 93, 3995, 4475
PscI ACATGT 1 cut(s) 115
PshBI ATTAAT 2 cut(s) 5010, 5118
PsiI TTATAA 3 cut(s) 2363, 3101, 5208
Psp124BI GAGCTC 1 cut(s) 3348
Psp5II RGGWCCY 3 cut(s) 93, 3995, 4475
Psp6I CCWGG 7 cut(s) 48, 235, 1514, 2994, 3019, 3228, 3921
PspCI CACGTG 1 cut(s) 1538
PspGI CCWGG 7 cut(s) 48, 235, 1514, 2994, 3019, 3228, 3921
PspN4I GGNNCC 9 cut(s) 589, 2093, 2125, 2405, 2993, 3996, 3997, 4782, 5164
PspOMI GGGCCC 1 cut(s) 2403
PspPPI RGGWCCY 3 cut(s) 93, 3995, 4475
PstI CTGCAG 1 cut(s) 499
PstNI CAGNNNCTG 2 cut(s) 218, 3650
PsuI RGATCY 9 cut(s) 338, 392, 587, 974, 1328, 1582, 1801, 2019, 4096
PvuII CAGCTG 2 cut(s) 215, 2317
RigI GGCCGGCC 1 cut(s) 2188
RseI CAYNNNNRTG 3 cut(s) 2763, 3012, 4920
SacI GAGCTC 1 cut(s) 3348
SalI GTCGAC 1 cut(s) 981
SatI GCNGC 9 cut(s) 213, 216, 798, 1773, 2782, 3651, 3903, 3906, 4362
SchI GAGTC 8 cut(s) 545, 1028, 1028, 1358, 2768, 3571, 3717, 5206
ScrFI CCNGG 7 cut(s) 50, 237, 1516, 2996, 3021, 3230, 3923
SduI GDGCHC 7 cut(s) 329, 934, 1087, 1281, 2407, 3157, 3348
SexAI ACCWGGT 1 cut(s) 3019
SfcI CTRYAG 3 cut(s) 495, 3190, 4275
SfuI TTCGAA 2 cut(s) 4838, 4874
SmiMI CAYNNNNRTG 3 cut(s) 2763, 3012, 4920
SmlI CTYRAG 5 cut(s) 1892, 2776, 3358, 3449, 3774
SmoI CTYRAG 5 cut(s) 1892, 2776, 3358, 3449, 3774
SseBI AGGCCT 1 cut(s) 2524
SspI AATATT 4 cut(s) 521, 697, 1443, 2701
SstI GAGCTC 1 cut(s) 3348
StuI AGGCCT 1 cut(s) 2524
StyD4I CCNGG 7 cut(s) 48, 235, 1514, 2994, 3019, 3228, 3921
StyI CCWWGG 3 cut(s) 2023, 2758, 4207
TaiI ACGT 7 cut(s) 870, 1540, 1563, 1941, 2219, 2556, 4835
TaqI TCGA 9 cut(s) 554, 982, 1722, 1988, 2421, 3759, 4838, 4874, 5043
TaqII GACCGA 1 cut(s) 5105
TatI WGTACW 3 cut(s) 375, 1783, 2319
TseFI GTSAC 6 cut(s) 548, 1043, 1259, 1352, 1501, 4955
TseI GCWGC 9 cut(s) 212, 215, 797, 1772, 2781, 3650, 3902, 3905, 4361
Tsp45I GTSAC 6 cut(s) 548, 1043, 1259, 1352, 1501, 4955
TspGWI ACGGA 4 cut(s) 1520, 3500, 3777, 4103
Van91I CCANNNNNTGG 2 cut(s) 335, 3001
Vha464I CTTAAG 1 cut(s) 3358
VneI GTGCAC 2 cut(s) 930, 1083
VspI ATTAAT 2 cut(s) 5010, 5118
XbaI TCTAGA 2 cut(s) 641, 4481
XceI RCATGY 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 982
XmnI GAANNNNTTC 3 cut(s) 2454, 2586, 5039
ZraI GACGTC 1 cut(s) 868
Zsp2I ATGCAT 2 cut(s) 561, 3820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.