Rroxscaffold_1G00029940

chromodomain-helicase-DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007957
Physical Location & Seq
Forward (+)
38968874 .. 38986184
17311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00029940.1

Sequence Viewer

Length: 5247 bp
ATGGCCTTTTTCAGGAACTACTCTAATGAAACAGTTTCACATAGTTTCCTGGAAGACCAGAGCCAGGGACCACGTGATGATAGAATTCACAGCTCCTCAGGGAATGCAGATGCACATGGAATGTCTTGTGATAAGGAGTTTGATATGAATATGGATGTGAAGTATCAAAGTGAAGATGAACAAGATGGTCCTAGTAGGTTGCAAAATGAGGCCGCTGCTGATGAGAGTGTTGGTGCCGGAGTGTCCAATTCCCAGTCTTCTGGGAGGAGGACGACTGTGGCTGGAAAGTGGGGTTCAACCTTTTGGAAGGATTGTCAACCTATGTGCCCTCAGGGTGGATCCGATTCTGGACAGGACACTAAAAGTGGGTCTGAGTACAGAAATGCGGTTGGATCTGAAGATAATTCTTCGGATGTCAGGGAAGATAGAGTAGATTCTGAAGATGATGGCAGACGTAAAGTACGAAAGGGACAACGGGGCCATTCTGATATACCTGCAGATGAAATGTTGTCTGATGAATATTATGAGCAGGATGGGGAAGAACAGAGTGACTCAATGCATTACAGAGGTTTTCATCATTCAGTTGGGTCCACTTCCAGGCCACAATCAAAGCCAGCCCCTGTCACCAATCACATGCTTAGGACTTCTAAACTTTCAAGTAATAATGGAAATTATGATGATGGCGAAGATGACAATGAAAATATTGATGCTGCTGATGCTGATTACGAAGAAGAGGATGAAGATGAAGATGATCCTGATGATGCAGATTTTGAACCTGATTATGGTGTTCCAAGTGGTCGCACTGCAAAGAAGGATAAAGATTGGAATGATCAAGATTCTGATGAAGATAATGACAGTGATGATGAACTTGACGTCTCAGATGAAGATAATTCTTTTTATGCGAAGAAACCAAAGGGTAGGCATCGTGGTAAAGGTGCACATACTGTTAAACTTAAAAGAGAACGCAAGTCATACCATGCATCTAGTCGACAAAGAAGAAGAAAGTCATCATTTGATGATGATGAGTCCTCAGCAGAGGACTCTGAGAGTGACAGTGATGAGGGTTTTAAAAGCACGAGAAGGAAAGGTGCACATATCCGCAAAAGTAATGGCCGGACAACAAATGTCTCTGGACGAAATGGTGAAGTACGAACTTCTACTAGGTCTGTTCGAAAGGTGTCATATGTTGAAAGTGAAGGAAGTGATGAAATTGATGAAGGCAAGAAAAAGAAGTCGCAGAAGGAGGAGATTGAGGAGGAGGATGGTGACTATATTGAAAAGGTGCTCTGGCATCAACCAAAGGGCATGGCTGAAGATGCTATTCAGAATAATAGATCCACGGAGCCTCTGTTGTTAAGTCACTTGTTTGACTCTGAGCCAGATTGGAGTAATACAGAATTCTTGATCAAATGGAAAGGCTTGTCACATTTGCACTGCCAGTGGAAATCATTTTCTGAGCTACAAAGTCTTAGCGGTTTTAAGAAGGTTTTAAACTACACTAAAAAGGTGATAGAGGATGCCAGGTACAGGAAGACCATCTCACGTGAAGAGATTGAGGTTCATGACGTAAGCAGGGAAATGGACTTAGATCTCATCAAGCAAAACAGTCAGGTGGAGAGAATAATTGCAGACCGCATCAAGCAAGATAGCTCAGGTGATGTAGTACCTGAGTATCTTGTCAAGTGGCAAGGATTGTCTTATGCTGAAGCGACATGGGAGAAGGATGTCGATATTGCATTTGCACAAGATGCTATTGATGAGTTCAAGGCTCGTGAGGCTGCAATGGCTGTACAAGGGAAAATGGTCGATCTCCAACGCAAGAAAAGCAAAGGAAGTCTAAGGAAGCTTGATGAACAGCCTGAATGGTTGAAAGGAGGAAAGCTTCGTGATTATCAGCTTGAGGGTTTGAATTTTCTTGTTAATAGTTGGAGAAATGATACCAATGTCATTTTAGCTGATGAAATGGGTCTTGGCAAAACTGTTCAATCAGTTTCGATGCTTGGTTTTTTACAGAATGCTCAACAGATTCATGGGCCATTTCTCGTTGTTGTACCATTATCTACTTTGTCGAATTGGGCAAAAGAATTTAGGAAATGGCTTCCTGATCTGAATGTTATAGTGTATGTTGGTACCCGTGCAAGCCGAGAGGTTTGTCAGCAGTATGAATTCAATAATGAGAAAATAGTTGGCCGGCCTATAAAATTTAATGCACTGTTGACAACATATGAAGTGGTTCTAAAGGATAAGGCTGTTCTTTCCAAGATCAAGTGGAATTATCTGATGGTGGATGAAGCTCATAGGCTAAAGAACAGTGAGGCTCAGCTGTACACAACTCTCTTGGAATTTAGCACAAAAAACAAGTTGCTTATAACTGGAACCCCATTGCAGAACAGTGTGGAAGAGTTATGGGCCCTGCTTCACTTCCTAGATCCCCACAAGTTCAATAACAAAGATGATTTTGTTCAGAACTACAAGAATCTCAGTTCATTTAATGAGATTGAGCTTGCTAATCTTCACATGGAACTGAGGCCTCACATTCTCCGAAGAGTTATTAAGGATGTTGAAAAGTCTTTACCTCCAAAAATTGAACGAATTCTTAGGGTTGAAATGTCTCCTCTTCAGAAACAGTACTACAAGTGGATTTTGGAGCGTAACTTCCATAACTTGAACAAAGGAGTTCGTGGGAATCAGGTTTCATTGTTGAATATTGTAGTAGAGTTGAAGAAATGCTGTAATCATCCCTTCCTTTTTGAAAGTGCTGACCATGGTTATGGTGGGGACTCAAGCAGTAAAGATGGCAGTAAATTGGAGAGAATTATTTTAAGCAGTGGGAAGTTGGTTATACTTGATAAGCTTCTTGTTAGATTACATGAGACGAAACACCGTGTTCTCATCTTTTCCCAGATGGTAAGAATGTTGGACATACTAGCCGAGTATATGTCGCATAGAGGGTTTCAATTTCAGCGACTTGATGGAAGTACAAAGGCTGATTTACGTCACCAGGCTATGGAACATTTCAATGCACCTGGTAGTGAAGATTTTTGCTTCCTTCTTTCAACACGGGCAGGTGGCTTGGGAATCAATCTTGCCACGGCGGACACGGTTATAATATTTGATTCGGATTGGAATCCTCAAAACGACTTGCAGGCAATGAGTAGAGCTCACAGAATTGGGCAACAGGAAGTTGTCAACATCTATAGATTTGTAACAAGCAAGAGTGTTGAGGAAGATATCTTGGAGCGAGCTAAGAAAAAGATGGTGCTAGACCACTTGGTGATTCAAAAGTTAAATGCAGAGGGAAGATTGGAGAAGAAAGAAACCAAAAAGGGGACTTTATTTGATAAAAATGAGCTATCTGCTATCTTAAGATTTGGAGCTGAGGAACTGTTTAAGGAAGAGAAAAATGAAGAAGAAAGCAAGAAAGGACTATTAAGTATGGATATTGATGAGATTCTTGAGAGGGCAGAAAAGGTTGAAGAGAAGGAAACTACAGAGGATGGGCATGAATTGCTGAGTGCTTTTAAGGTTGCAAATTTTGGTACTGCTGAAGATGATGGGAGTTTTTGGAGTCGTTGGATAAAGCCAGAAGCAGTGTCCCAAGCTGAAGATGCTCTTGCTCCTCGCGCTACAAGGAATACCAAGAGCTATGCAGAAGCTGCTCAGCCTGATAGGAGCAATAAAAGGAAAAAGAAAGAATCTGAGCCTCAGGAAAGAGTCCAGAAAAGGCGCAAACCAGATCATTCAGTTCCATCAGCACCAATGATCGACGGAGCATCTGCTCAAGTAAGAGGATGGTCATCTGGAAATGTGTCTAAACGAGATGCATTGCGGTTTTCCCGTGCGGTTATGAAGTTTGGGAATGAGAGCCAAATTGGCTTAATAGTTGAGGAGGTTGGTGGCGCAATTGCAGCAGCTTCACCTGAAGCCCAGGTTGAGCTTTTCAATGCATTGATCGATGGATGCAGAGAAGCAGTGGAAGTGGGGAGTTTGGACCAGAAGGGACCCTTGCTTGATTTTTTTGGTGTCCCTGTGAAGGCTAGTGATCTGGTCAACCGCGTTCAAGAACTTCAGCTCCTGGCAAAGCGCATTATTCGATATGAAGACCCCATTGGACAGTTCCGTGTCCTGATGTACCTCAAACCTTCAAACTGGTCAAAAGGTTGTGGCTGGAATCAGATTGATGATGCAAGGTTACTTCTCGGAATATACTACCATGGGTTTGGCAATTGGGAAAAGATAAGATTAGATGAAAGGCTTGGCCTTATGAAGAAGATTGCTCCTGTAGAACTTCAGCACCATGAAACTTTCTTACCCCGTGCTCCAAATTTGAGAGACCGTGCTAATGCTCTTTTGGAAATGGAACTTGCAGCTCTTGGGGGAAAGAGCGCAAATGCTAAAGTTGGTCGTAAGGCTTCCAGAGAGAGGGAAAATCCTGTCCCTGTTTCTGTGTCTCGCACTGGGGTCAAGAAAGGAAAAGCAGGTTCTTCTAGACTAAATGTCCAAATGAACAAGGACAAACCGCTGAAACCCCAGAGAGTTGAACCTTTAGTGAAAGAAGAGGGTGAAATGTCTGACGATGAAGAAGTATATGAGAAGTTTAAGGAAGAGAAATGGATGGAATGGTGTGAAGAGATGATGACTAGTGAAATTAAAACCCTGAATCGTCTTCATCGGTTGCAGACGACTAGTGCCAATCTTCCAAAGGAGAAGGTGCTTTCAAAAATTCGAAATTATTTACAACTTCTTGGACGAAGGATAGATCAAATTGTTTTGGACAATGAGGAGGAACCATATGGACAGGATAGGATGACCACGCGCTTGTGGAACTTTGTTTCTACATTTTCAAACCTGTCTGGAGAGAGACTTCACCAAATATATGCGAAACTTAAACAAGAGCAGGATGAGGAACCTGGTCCTTCCCACATCAATGGCTCTGCATCTGGTCCTTTTGGCAGAGACAGTGACCCTACTTCCTTCTCCCATCTTGCTGAGAGGCAAAGAGGGTACAAAAGTGTTAATAACCAAACGTTTGAACCTCTGAAGGGTTTTGATACAGCTAAATTTGAAGCATGGAAACGAAGGAGAAGAGGGGAGACTGATAGTCTATCTCAAAGACCTTTGAGTAATGGTTCTCGGCCAGCTGATCCAAACTCGATGGGAATTCTTGGTGCAGGACCATCTGAAAATAGACGCTCTGTCAATGAAAAGCCTTACAAGACTCGTCAAACTGGTTTACCACCAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chromo DUF4208 Helicase_C SLIDE SNF2_N

Protein Analysis

1748

Amino Acids

198.87

Weight (kDa)

5.86

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chromo PF00385 537 - 591 1.4e-14 Chromo (CHRromatin Organisation MOdifier) domain
ResIII PF04851 627 - 794 9.5e-11 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 631 - 915 1.8e-86 SNF2-related domain
DEAD PF00270 642 - 799 2.3e-07 DEAD/DEAH box helicase
HDA2-3 PF11496 866 - 1090 2.8e-11 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 945 - 1058 2.8e-26 Helicase conserved C-terminal domain
HTH_CHD1_Hrp3 PF23588 1370 - 1451 8e-23 ATP-dependent helicase CHD1-2/hrp3 HTH domain
CHD1-like_C PF13907 1534 - 1628 9.2e-16 Chromodomain-helicase-DNA-binding protein 1-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2369, 3107
AarI CACCTGC 1 cut(s) 3056
AatII GACGTC 1 cut(s) 876
Acc36I ACCTGC 3 cut(s) 502, 3056, 4469
Acc65I GGTACC 1 cut(s) 2129
AccB1I GGYRCC 2 cut(s) 233, 2129
AccB7I CCANNNNNTGG 1 cut(s) 3007
AccI GTMKAC 1 cut(s) 988
AccII CGCG 3 cut(s) 3624, 4056, 4816
AclI AACGTT 1 cut(s) 5027
AclWI GGATC 7 cut(s) 333, 346, 400, 746, 1329, 2423, 5138
AcoI YGGCCR 3 cut(s) 1111, 2188, 5135
AcvI CACGTG 2 cut(s) 74, 1544
AcyI GRCGYC 1 cut(s) 873
AdeI CACNNNGTG 1 cut(s) 3274
AflII CTTAAG 1 cut(s) 3364
AhdI GACNNNNNGTC 3 cut(s) 4496, 5100, 5195
AhlI ACTAGT 2 cut(s) 4640, 4685
AjnI CCWGG 9 cut(s) 48, 63, 596, 1520, 3000, 3025, 3927, 4074, 4909
AjuI GAANNNNNNNTTGG 4 cut(s) 1953, 1985, 4092, 4124
AloI GAACNNNNNNTCC 2 cut(s) 4056, 4088
Alw21I GWGCWC 5 cut(s) 940, 1093, 1287, 3163, 4321
Alw26I GTCTC 9 cut(s) 880, 1132, 2616, 2867, 4326, 4455, 4855, 4950, 5087
Alw44I GTGCAC 2 cut(s) 936, 1089
AlwI GGATC 7 cut(s) 333, 346, 400, 746, 1329, 2423, 5138
AlwNI CAGNNNCTG 3 cut(s) 620, 3656, 4075
ApaI GGGCCC 1 cut(s) 2413
ApaLI GTGCAC 2 cut(s) 936, 1089
ApeKI GCWGC 7 cut(s) 215, 710, 1778, 3656, 3908, 3911, 4367
Asp700I GAANNNNTTC 2 cut(s) 2232, 2592
Asp718I GGTACC 1 cut(s) 2129
AspLEI GCGC 6 cut(s) 3626, 3729, 3902, 4086, 4388, 4818
AsuII TTCGAA 2 cut(s) 1171, 4726
AvaII GGWCC 8 cut(s) 68, 188, 588, 3991, 4001, 4913, 4943, 5174
AxyI CCTNAGG 3 cut(s) 97, 330, 3705
BaeGI GKGCMC 4 cut(s) 329, 940, 1093, 2413
BaeI ACNNNNGTAYC 6 cut(s) 1655, 1688, 1929, 1962, 4996, 5029
BamHI GGATCC 1 cut(s) 338
BanI GGYRCC 2 cut(s) 233, 2129
BanII GRGCYC 2 cut(s) 2413, 3163
BauI CACGAG 2 cut(s) 1075, 1770
BbrPI CACGTG 2 cut(s) 74, 1544
BbsI GAAGAC 5 cut(s) 60, 249, 1538, 4107, 4658
Bbv12I GWGCWC 5 cut(s) 940, 1093, 1287, 3163, 4321
BbvCI CCTCAGC 2 cut(s) 1030, 3378
BbvI GCAGC 7 cut(s) 202, 697, 1765, 3643, 3920, 3923, 4379
BceAI ACGGC 1 cut(s) 3108
BciT130I CCWGG 9 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076, 4911
BclI TGATCA 2 cut(s) 829, 1404
BcoDI GTCTC 9 cut(s) 880, 1132, 2616, 2867, 4326, 4455, 4855, 4950, 5087
BcuI ACTAGT 2 cut(s) 4640, 4685
BfmI CTRYAG 4 cut(s) 495, 3196, 3489, 4281
BfrI CTTAAG 1 cut(s) 3364
BfuAI ACCTGC 3 cut(s) 502, 3056, 4469
BglI GCCNNNNNGGC 1 cut(s) 3873
BglII AGATCT 1 cut(s) 1588
BisI GCNGC 8 cut(s) 213, 216, 711, 1779, 3657, 3909, 3912, 4368
BlpI GCTNAGC 2 cut(s) 2319, 3660
BlsI GCNGC 8 cut(s) 214, 217, 712, 1780, 3658, 3910, 3913, 4369
BmcAI AGTACT 1 cut(s) 2630
Bme1390I CCNGG 9 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076, 4911
Bme18I GGWCC 8 cut(s) 68, 188, 588, 3991, 4001, 4913, 4943, 5174
BmeRI GACNNNNNGTC 3 cut(s) 4496, 5100, 5195
BmrFI CCNGG 9 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076, 4911
BmrI ACTGGG 2 cut(s) 247, 4467
BmuI ACTGGG 2 cut(s) 247, 4467
BpiI GAAGAC 5 cut(s) 60, 249, 1538, 4107, 4658
BplI GAGNNNNNCTC 2 cut(s) 3145, 3177
BpmI CTGGAG 1 cut(s) 4875
Bpu10I CCTNAGC 4 cut(s) 638, 1030, 1651, 3378
Bpu1102I GCTNAGC 2 cut(s) 2319, 3660
Bpu14I TTCGAA 2 cut(s) 1171, 4726
BpuEI CTTGAG 4 cut(s) 1919, 2767, 3476, 3765
Bsa29I ATCGAT 1 cut(s) 3954
BsaAI YACGTR 2 cut(s) 74, 1544
BsaBI GATNNNNATC 2 cut(s) 3126, 3796
BsaHI GRCGYC 1 cut(s) 873
BsaI GGTCTC 1 cut(s) 4326
BsaJI CCNNGG 6 cut(s) 64, 1338, 2764, 3090, 3927, 4213
BsaXI ACNNNNNCTCC 4 cut(s) 1334, 1364, 4056, 4086
Bse118I RCCGGY 1 cut(s) 2190
Bse1I ACTGG 6 cut(s) 253, 1438, 2377, 4154, 4462, 5233
Bse21I CCTNAGG 3 cut(s) 97, 330, 3705
Bse3DI GCAATG 4 cut(s) 1788, 2381, 3156, 3824
Bse8I GATNNNNATC 2 cut(s) 3126, 3796
BseBI CCWGG 9 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076, 4911
BseCI ATCGAT 1 cut(s) 3954
BseDI CCNNGG 6 cut(s) 64, 1338, 2764, 3090, 3927, 4213
BseJI GATNNNNATC 2 cut(s) 3126, 3796
BseMI GCAATG 4 cut(s) 1788, 2381, 3156, 3824
BseNI ACTGG 6 cut(s) 253, 1438, 2377, 4154, 4462, 5233
BseRI GAGGAG 9 cut(s) 85, 280, 1259, 1268, 1271, 2604, 3609, 3902, 4796
BseSI GKGCMC 4 cut(s) 329, 940, 1093, 2413
BseXI GCAGC 7 cut(s) 202, 697, 1765, 3643, 3920, 3923, 4379
BsgI GTGCAG 1 cut(s) 5190
Bsh1236I CGCG 3 cut(s) 3624, 4056, 4816
BshNI GGYRCC 2 cut(s) 233, 2129
BshVI ATCGAT 1 cut(s) 3954
BsiHKAI GWGCWC 5 cut(s) 940, 1093, 1287, 3163, 4321
BsiSI CCGG 3 cut(s) 237, 1114, 2191
BslFI GGGAC 8 cut(s) 81, 483, 2792, 3343, 3580, 4010, 4014, 4421
BsmAI GTCTC 9 cut(s) 880, 1132, 2616, 2867, 4326, 4455, 4855, 4950, 5087
BsmBI CGTCTC 2 cut(s) 880, 2867
BsmFI GGGAC 8 cut(s) 81, 483, 2792, 3343, 3580, 4010, 4014, 4421
BsmI GAATGC 2 cut(s) 109, 2020
Bso31I GGTCTC 1 cut(s) 4326
Bsp119I TTCGAA 2 cut(s) 1171, 4726
Bsp120I GGGCCC 1 cut(s) 2409
Bsp1286I GDGCHC 7 cut(s) 329, 940, 1093, 1287, 2413, 3163, 4321
Bsp1407I TGTACA 2 cut(s) 1789, 2325
Bsp1720I GCTNAGC 2 cut(s) 2319, 3660
Bsp19I CCATGG 2 cut(s) 2764, 4213
BspDI ATCGAT 1 cut(s) 3954
BspFNI CGCG 3 cut(s) 3624, 4056, 4816
BspHI TCATGA 1 cut(s) 1561
BspMAI CTGCAG 1 cut(s) 499
BspMI ACCTGC 3 cut(s) 502, 3056, 4469
BspPI GGATC 7 cut(s) 333, 346, 400, 746, 1329, 2423, 5138
BspT104I TTCGAA 2 cut(s) 1171, 4726
BspT107I GGYRCC 2 cut(s) 233, 2129
BspTI CTTAAG 1 cut(s) 3364
BspTNI GGTCTC 1 cut(s) 4326
BsrDI GCAATG 4 cut(s) 1788, 2381, 3156, 3824
BsrFI RCCGGY 1 cut(s) 2190
BsrGI TGTACA 2 cut(s) 1789, 2325
BsrI ACTGG 6 cut(s) 253, 1438, 2377, 4154, 4462, 5233
BssAI RCCGGY 1 cut(s) 2190
BssECI CCNNGG 6 cut(s) 64, 1338, 2764, 3090, 3927, 4213
BssNI GRCGYC 1 cut(s) 873
BssSI CACGAG 2 cut(s) 1075, 1770
BssT1I CCWWGG 2 cut(s) 2764, 4213
Bst2BI CACGAG 2 cut(s) 1075, 1770
Bst2UI CCWGG 9 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076, 4911
BstACI GRCGYC 1 cut(s) 873
BstAFI CTTAAG 1 cut(s) 3364
BstAPI GCANNNNNTGC 3 cut(s) 1748, 3508, 3656
BstAUI TGTACA 2 cut(s) 1789, 2325
BstBAI YACGTR 2 cut(s) 74, 1544
BstBI TTCGAA 2 cut(s) 1171, 4726
BstC8I GCNNGC 7 cut(s) 615, 2140, 2192, 2505, 3147, 3243, 5139
BstDSI CCRYGG 4 cut(s) 1338, 2764, 3090, 4213
BstENI CCTNNNNNAGG 1 cut(s) 10
BstFNI CGCG 3 cut(s) 3624, 4056, 4816
BstHHI GCGC 6 cut(s) 3626, 3729, 3902, 4086, 4388, 4818
BstMAI GTCTC 9 cut(s) 880, 1132, 2616, 2867, 4326, 4455, 4855, 4950, 5087
BstNI CCWGG 9 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076, 4911
BstNSI RCATGY 1 cut(s) 637
BstSCI CCNGG 9 cut(s) 48, 63, 596, 1520, 3000, 3025, 3927, 4074, 4909
BstSFI CTRYAG 4 cut(s) 495, 3196, 3489, 4281
BstSLI GKGCMC 4 cut(s) 329, 940, 1093, 2413
BstUI CGCG 3 cut(s) 3624, 4056, 4816
BstV1I GCAGC 7 cut(s) 202, 697, 1765, 3643, 3920, 3923, 4379
BstV2I GAAGAC 5 cut(s) 60, 249, 1538, 4107, 4658
BstX2I RGATCY 5 cut(s) 338, 392, 1334, 1588, 2428
BstXI CCANNNNNNTGG 4 cut(s) 260, 2771, 4220, 4928
BstYI RGATCY 5 cut(s) 338, 392, 1334, 1588, 2428
Bsu15I ATCGAT 1 cut(s) 3954
Bsu36I CCTNAGG 3 cut(s) 97, 330, 3705
BsuTUI ATCGAT 1 cut(s) 3954
BtgI CCRYGG 4 cut(s) 1338, 2764, 3090, 4213
BtsI GCAGTG 5 cut(s) 801, 1432, 2833, 3597, 3978
BveI ACCTGC 3 cut(s) 502, 3056, 4469
Cac8I GCNNGC 7 cut(s) 615, 2140, 2192, 2505, 3147, 3243, 5139
CaiI CAGNNNCTG 3 cut(s) 620, 3656, 4075
CciI TCATGA 1 cut(s) 1561
CfoI GCGC 6 cut(s) 3626, 3729, 3902, 4086, 4388, 4818
Cfr10I RCCGGY 1 cut(s) 2190
ClaI ATCGAT 1 cut(s) 3954
CseI GACGC 1 cut(s) 5199
CsiI ACCWGGT 2 cut(s) 3025, 4909
DraI TTTAAA 2 cut(s) 1069, 1491
DraIII CACNNNGTG 1 cut(s) 3274
DriI GACNNNNNGTC 3 cut(s) 4496, 5100, 5195
EaeI YGGCCR 3 cut(s) 1111, 2188, 5135
Eam1105I GACNNNNNGTC 3 cut(s) 4496, 5100, 5195
EciI GGCGGA 1 cut(s) 3110
Ecl136II GAGCTC 1 cut(s) 3161
Eco130I CCWWGG 2 cut(s) 2764, 4213
Eco147I AGGCCT 1 cut(s) 2530
Eco24I GRGCYC 2 cut(s) 2413, 3163
Eco31I GGTCTC 1 cut(s) 4326
Eco32I GATATC 1 cut(s) 3232
Eco47I GGWCC 8 cut(s) 68, 188, 588, 3991, 4001, 4913, 4943, 5174
Eco53kI GAGCTC 1 cut(s) 3161
Eco72I CACGTG 2 cut(s) 74, 1544
Eco81I CCTNAGG 3 cut(s) 97, 330, 3705
EcoICRI GAGCTC 1 cut(s) 3161
EcoNI CCTNNNNNAGG 1 cut(s) 10
EcoO109I RGGNCCY 2 cut(s) 2410, 4001
EcoRI GAATTC 5 cut(s) 84, 1397, 2165, 2592, 5160
EcoRII CCWGG 9 cut(s) 48, 63, 596, 1520, 3000, 3025, 3927, 4074, 4909
EcoRV GATATC 1 cut(s) 3232
EcoT14I CCWWGG 2 cut(s) 2764, 4213
EcoT22I ATGCAT 4 cut(s) 561, 982, 3826, 3949
EcoT38I GRGCYC 2 cut(s) 2413, 3163
ErhI CCWWGG 2 cut(s) 2764, 4213
Esp3I CGTCTC 2 cut(s) 880, 2867
FalI AAGNNNNNCTT 4 cut(s) 3597, 3629, 3989, 4021
FaqI GGGAC 8 cut(s) 81, 483, 2792, 3343, 3580, 4010, 4014, 4421
FauNDI CATATG 3 cut(s) 1183, 2224, 4792
FbaI TGATCA 2 cut(s) 829, 1404
FblI GTMKAC 1 cut(s) 988
Fnu4HI GCNGC 8 cut(s) 213, 216, 711, 1779, 3657, 3909, 3912, 4368
FriOI GRGCYC 2 cut(s) 2413, 3163
FseI GGCCGGCC 1 cut(s) 2194
Fsp4HI GCNGC 8 cut(s) 213, 216, 711, 1779, 3657, 3909, 3912, 4368
GlaI GCGC 6 cut(s) 3625, 3728, 3901, 4085, 4387, 4817
GluI GCNGC 8 cut(s) 213, 216, 711, 1779, 3657, 3909, 3912, 4368
GsuI CTGGAG 1 cut(s) 4875
HapII CCGG 3 cut(s) 237, 1114, 2191
HgaI GACGC 1 cut(s) 5199
HhaI GCGC 6 cut(s) 3626, 3729, 3902, 4086, 4388, 4818
Hin1I GRCGYC 1 cut(s) 873
Hin6I GCGC 6 cut(s) 3624, 3727, 3900, 4084, 4386, 4816
HinP1I GCGC 6 cut(s) 3624, 3727, 3900, 4084, 4386, 4816
HincII GTYRAC 5 cut(s) 317, 989, 2217, 3190, 4051
HindII GTYRAC 5 cut(s) 317, 989, 2217, 3190, 4051
HindIII AAGCTT 3 cut(s) 1844, 1880, 2852
HpaII CCGG 3 cut(s) 237, 1114, 2191
Hpy99I CGWCG 1 cut(s) 3770
HpyCH4IV ACGT 7 cut(s) 73, 454, 873, 1543, 1566, 2995, 5027
HpySE526I ACGT 7 cut(s) 73, 454, 873, 1543, 1566, 2995, 5027
Hsp92I GRCGYC 1 cut(s) 873
HspAI GCGC 6 cut(s) 3624, 3727, 3900, 4084, 4386, 4816
KflI GGGWCCC 1 cut(s) 4001
KpnI GGTACC 1 cut(s) 2133
KroI GCCGGC 1 cut(s) 2190
KroNI GCCGGC 1 cut(s) 2192
Ksp22I TGATCA 2 cut(s) 829, 1404
Lsp1109I GCAGC 7 cut(s) 202, 697, 1765, 3643, 3920, 3923, 4379
MabI ACCWGGT 2 cut(s) 3025, 4909
MaeII ACGT 7 cut(s) 73, 454, 873, 1543, 1566, 2995, 5027
MfeI CAATTG 2 cut(s) 3903, 4225
MflI RGATCY 5 cut(s) 338, 392, 1334, 1588, 2428
MhlI GDGCHC 7 cut(s) 329, 940, 1093, 1287, 2413, 3163, 4321
MlyI GAGTC 8 cut(s) 545, 1034, 1034, 1364, 2774, 3577, 3723, 5212
MmeI TCCRAC 5 cut(s) 370, 1837, 1907, 2898, 3554
Mph1103I ATGCAT 4 cut(s) 561, 982, 3826, 3949
MroNI GCCGGC 1 cut(s) 2190
MroXI GAANNNNTTC 2 cut(s) 2232, 2592
MslI CAYNNNNRTG 3 cut(s) 2769, 3018, 4926
MspA1I CMGCKG 4 cut(s) 215, 2323, 4522, 5141
MspCI CTTAAG 1 cut(s) 3364
MspI CCGG 3 cut(s) 237, 1114, 2191
MspR9I CCNGG 9 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076, 4911
MunI CAATTG 2 cut(s) 3903, 4225
Mva1269I GAATGC 2 cut(s) 109, 2020
MvaI CCWGG 9 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076, 4911
MvnI CGCG 3 cut(s) 3624, 4056, 4816
NaeI GCCGGC 1 cut(s) 2192
NcoI CCATGG 2 cut(s) 2764, 4213
NdeI CATATG 3 cut(s) 1183, 2224, 4792
NgoMIV GCCGGC 1 cut(s) 2190
NmeAIII GCCGAG 3 cut(s) 2168, 2956, 5113
NmuCI GTSAC 8 cut(s) 548, 622, 1049, 1265, 1358, 1422, 2996, 4961
NsiI ATGCAT 4 cut(s) 561, 982, 3826, 3949
NspI RCATGY 1 cut(s) 637
NspV TTCGAA 2 cut(s) 1171, 4726
PagI TCATGA 1 cut(s) 1561
PaqCI CACCTGC 1 cut(s) 3056
PceI AGGCCT 1 cut(s) 2530
PcsI WCGNNNNNNNCGW 1 cut(s) 460
PctI GAATGC 2 cut(s) 109, 2020
PdiI GCCGGC 1 cut(s) 2192
PdmI GAANNNNTTC 2 cut(s) 2232, 2592
PflMI CCANNNNNTGG 1 cut(s) 3007
PfoI TCCNGGA 1 cut(s) 48
PkrI GCNGC 8 cut(s) 214, 217, 712, 1780, 3658, 3910, 3913, 4369
PleI GAGTC 8 cut(s) 545, 1033, 1034, 1364, 2774, 3576, 3722, 5212
PmaCI CACGTG 2 cut(s) 74, 1544
PmlI CACGTG 2 cut(s) 74, 1544
PpsI GAGTC 8 cut(s) 545, 1033, 1034, 1364, 2774, 3576, 3722, 5212
Ppu21I YACGTR 2 cut(s) 74, 1544
PpuMI RGGWCCY 1 cut(s) 4001
PsiI TTATAA 2 cut(s) 2369, 3107
Psp124BI GAGCTC 1 cut(s) 3163
Psp1406I AACGTT 1 cut(s) 5027
Psp5II RGGWCCY 1 cut(s) 4001
Psp6I CCWGG 9 cut(s) 48, 63, 596, 1520, 3000, 3025, 3927, 4074, 4909
PspCI CACGTG 2 cut(s) 74, 1544
PspGI CCWGG 9 cut(s) 48, 63, 596, 1520, 3000, 3025, 3927, 4074, 4909
PspOMI GGGCCC 1 cut(s) 2409
PspPPI RGGWCCY 1 cut(s) 4001
PstI CTGCAG 1 cut(s) 499
PstNI CAGNNNCTG 3 cut(s) 620, 3656, 4075
PsuI RGATCY 5 cut(s) 338, 392, 1334, 1588, 2428
PvuII CAGCTG 2 cut(s) 2323, 5141
RigI GGCCGGCC 1 cut(s) 2194
RseI CAYNNNNRTG 3 cut(s) 2769, 3018, 4926
SacI GAGCTC 1 cut(s) 3163
SalI GTCGAC 1 cut(s) 987
SatI GCNGC 8 cut(s) 213, 216, 711, 1779, 3657, 3909, 3912, 4368
ScaI AGTACT 1 cut(s) 2630
SchI GAGTC 8 cut(s) 545, 1034, 1034, 1364, 2774, 3577, 3723, 5212
ScrFI CCNGG 9 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076, 4911
SduI GDGCHC 7 cut(s) 329, 940, 1093, 1287, 2413, 3163, 4321
SexAI ACCWGGT 2 cut(s) 3025, 4909
SfcI CTRYAG 4 cut(s) 495, 3196, 3489, 4281
SfuI TTCGAA 2 cut(s) 1171, 4726
SinI GGWCC 8 cut(s) 68, 188, 588, 3991, 4001, 4913, 4943, 5174
SmiMI CAYNNNNRTG 3 cut(s) 2769, 3018, 4926
SmlI CTYRAG 5 cut(s) 1898, 2782, 3364, 3455, 3780
SmoI CTYRAG 5 cut(s) 1898, 2782, 3364, 3455, 3780
SpeI ACTAGT 2 cut(s) 4640, 4685
SseBI AGGCCT 1 cut(s) 2530
SspI AATATT 4 cut(s) 521, 703, 2707, 3111
SstI GAGCTC 1 cut(s) 3163
StuI AGGCCT 1 cut(s) 2530
StyD4I CCNGG 9 cut(s) 48, 63, 596, 1520, 3000, 3025, 3927, 4074, 4909
StyI CCWWGG 2 cut(s) 2764, 4213
TaiI ACGT 7 cut(s) 76, 457, 876, 1546, 1569, 2998, 5030
TatI WGTACW 5 cut(s) 375, 1789, 2325, 2628, 2978
TauI GCSGC 1 cut(s) 215
TseFI GTSAC 8 cut(s) 548, 622, 1049, 1265, 1358, 1422, 2996, 4961
TseI GCWGC 7 cut(s) 215, 710, 1778, 3656, 3908, 3911, 4367
Tsp45I GTSAC 8 cut(s) 548, 622, 1049, 1265, 1358, 1422, 2996, 4961
TspGWI ACGGA 3 cut(s) 1355, 3783, 4109
Van91I CCANNNNNTGG 1 cut(s) 3007
Vha464I CTTAAG 1 cut(s) 3364
VneI GTGCAC 2 cut(s) 936, 1089
VpaK11BI GGWCC 8 cut(s) 68, 188, 588, 3991, 4001, 4913, 4943, 5174
XagI CCTNNNNNAGG 1 cut(s) 10
XbaI TCTAGA 1 cut(s) 4487
XceI RCATGY 1 cut(s) 637
XcmI CCANNNNNNNNNTGG 1 cut(s) 2771
XmiI GTMKAC 1 cut(s) 988
XmnI GAANNNNTTC 2 cut(s) 2232, 2592
ZraI GACGTC 1 cut(s) 874
ZrmI AGTACT 1 cut(s) 2630
Zsp2I ATGCAT 4 cut(s) 561, 982, 3826, 3949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.