Rh5DG358700

chromodomain-helicase-DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007957
Physical Location & Seq
Reverse (-)
53423731 .. 53441950
18220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG358700.1

Sequence Viewer

Length: 4998 bp
ATGGCCTTTTTCAGGAACTACTCTAATGAAACAGTTTCACATAGTTTCCTGGAAGACCAGAGCCAGGGACCACGTGATGATAGAATTCACAGCTCCTCAGGGAATGCGGATGCACATGGAATGTCTTGTGATAAGGAGTTTGATATGAATATGGATGTGAAGTATCAAAGTGAAGATGAACAAGATGGTCCTAGTAGGTTGCAAAATGAGGCAGCTGCTGATGAGGGTGCCGGTGCAGGAGTGTCCAATTCCCAGTCTTCTGGGAGGAGGACGACTGTGGCTGGAAAATGGGGTTCAACCTTTTGGAAGGATTGTCAACCTATGTGCCCTCAGGGTGGATCCGATTCTGGACAGGACACTAAAAGTGGGTCTGAGTACAGAAACGCTGTTGGATCTGAAGATAATTCTTCGGATGTCAGGGAAGATAGATTAGATTCTGAAGATGATGGCAGACCTAAAGTACGAAAGGGACAACGGGGCCATTCTGATATACCTGCAGATGAAATGTTGTCTGATGAATATTATGAGCAGGATGGGGAAGAACAGAGTGACTCAATGCATTATAGAGGGTTTCATCATTCAGTTGGGTCCACTTCCAGGCCACAATCAAAGCCAGCCCCTGTCACCAATCACATGCTTAGGACTTCTAAACTTTCAAATAATAATGGAAATTATGATGATGGCGAAGATGACAATGAAAATATTGATGCTGCTGATGCTGATTACGAAGAAGAGGATGAAGATGAAGATGATCCTGATGATGCAGATTTTGAACCTGATTATGGTGTTCCAAGTGGTCGCACTGCAAAGAAGGATAAAGATTGGAATGATCAAGATTCTGATGAAGATAATGACAGTGATGATGAACTTGACGTCTCAGATGAAGATAATTCTTTTTATGCGAAGAAACCAAAGGGTAGGCATCGTGGTAAAGGTGCACATACTGTTAAACTTAAAAGAGAACGCAAATCATACCATGGATCTAGTCGACAAAGAAGAAGAAAGTCATCATTTGATGATGATGAGTCCTCAGCAGAGGACTCTGAGAGTGACAGTGATGAGGGTTTTAAAAGCACGAGAAGGAAAGGTGCACATATCCGCAAAAGTAATGGCCGGACAACAAATGTCTCTGGACGAAATGGTGAAGTACGAACTTCTACTAGGTCTGTTCGAAAGGTGTCATATGTTGAAAGTGAAGGAAGTGATGAAATTGATGAAGGCAAGAAAAAGAAGTCGCAAAAGGAGGAGATTGAGGAGGAGGATGGTGACTATATTGAAAGGGTGCTCTGGCATCAACCAAAGGGCATGGCTGAAGATGCTATTCGGAATAATAGATCCACGGAGCCTCTGTTGTTAAGTCACTTGTTTGACTCTGAGCCAGATTGGAGTAATACAGAATTCTTGATCAAATGGAAAGGCTTGTCACATTTGCACTGCCAGTGGAAATCATTTTCTGAGCTACAAAGTCTTAGCGGTTTTAAGAAGGTTTTAAACTACACTAAAAAGGTGATGGAGGATGCCAGGTACAGGAAGACCATCTCACGTGAAGAGATTGAGGTTCATGACGTGAGCAGGGAAATGGACTTGGATCTCATCAAGCAAAACAGTCAGGTCGAGAGAATAATTGCAGACCGCATCAAGCAAGATGGCTCAGGTGATGTAGTACCTGAGTATCTTGTCAAGTGGCAAGGATTGTCTTATGCTGAAGCGACATGGGAGAAGGACGTTGATATTGCATTTGCACAAGATGCTATTGATGAGTTCAAGGCTCGTGAGGCTGCAATGGCTGTACAAGGGAAAATGGTCGATCTCCAACGCAAGAAAAGCAAAGGAAGTCTAAGGAAGCTTGATGAACAGCCTGAATGGTTGAAAGGAGGAAAGCTTCGTGATTATCAGCTTGAGGGTTTGAATTTTCTTGTTAATAGTTGGAGAAATGATACCAATGTCATTTTAGCTGATGAAATGGGTCTTGGCAAAACTGTTCAATCAGTTTCGATGCTTGGTTTTTTACAGAATGCTCAACAGATTCATGGGCCATTTCTCGTTGTTGTACCATTATCTACTTTGTCAAATTGGGCAAAAGAATTTAGGAAATGGCTTCCTGAGCTGAATGTTATTGTGTATGTTGGTACCCGTGCAAGCCGAGAGGTTTGTCAGCAGTATGAATTCAATAATGAGAAAATAGTTGGCCGGCCTATAAAATTTAATGCACTGTTGACAACATATGAAGTGGTTCTAAAGGATAAGGCTGTTCTTTCCAAGATCAAGTGGAATTATCTGATGGTGGATGAAGCTCATAGGCTAAAGAACAGTGAGGCTCAGCTGTACACAACTCTCTTGGAATTTAGCACAAAAAACAAGTTGCTTATAACCGGAACCCCATTGCAGAACAGTGTGGAAGAGTTATGGGCCCTGCTTCACTTCCTAGATCCCCACAAGTTCAATAACAAAGATGATTTTGTTCAGAACTACAAGAATCTCAGTTCATTTAATGAGATTGAGCTTGCTAATCTTCACATGGAACTGAGGCCTCACATTCTCCGAAGAGTTATTAAGGATGTTGAAAAGTCTTTACCTCCAAAAATTGAACGAATTCTTAGGGTTGAAATGTCTCCTCTTCAGAAACAGTACTACAAGTGGATTTTGGAGCGCAACTTCCATAACTTGAACAAAGGAGTTCGTGGGAATCAGGTTTCATTGTTGAATATTGTAGTAGAGTTGAAGAAATGCTGTAATCATCCCTTCCTTTTTGAAAGTGCTGACCATGGTTATGGTGGGGACTCAAGCAGTAAAGATGGCAGTAAATTGGAGAGAGTTATTTTAAGCAGTGGGAAGTTGGTTATACTTGATAAGCTTCTTGTTAGATTACATGAGACGAAACACCGTGTTCTCATCTTTTCCCAGATGGTAAGAATGTTGGACATACTAGCCGAGTATATGTCGCATAGAGGGTTTCAATTTCAGCGACTTGATGGAAGTACAAAGGCTGATTTACGTCACCAGGCTATGGAACATTTCAATGCACCTGGTAGTGAAGATTTTTGCTTCCTTCTTTCAACACGGGCAGGTGGCTTGGGAATCAACCTTGCCACGGCGGACACGGTTATAATATTTGATTCGGATTGGAATCCTCAAAATGACTTGCAGGCAATGAGTAGAGCTCACAGAATTGGGCAACAGGAAGTTGTCAACATCTATAGATTTGTAACAAGCAAGAGTGTTGAGGAAGATATCTTGGAGCGAGCTAAGAAAAAGATGGTGCTAGACCATTTGGTGATTCAAAAGTTAAACGCAGAGGGAAGATTGGAGAAGAAAGAAACCAAAAAGGGGACATTATTTGATAAAAATGAGCTATCTGCTATCTTAAGATTTGGAGCTGAGGAACTGTTTAAAGAAGAGAAAAATGAAGAAGAAAGCAAGAAAGGACTATTAAGTATGGATATTGATGAGATTCTTGAGAGGGCAGAAAAGGTTGAAGAGAAGGAAACTACAGAGGATGGCCATGAATTGCTGAGTGCTTTTAAGGTTGCAAATTTTGGTACTGCTGAAGATGATGGGAGTTTTTGGAGTCGTTGGATAAAGCCAGAAGCAGTGTCCCAAGCTGAAGATGCTCTTGCTCCTCGCGCTACAAGGAATACCAAGAGCTATGCAGAAGCTGCTCAGCCTGATAGGAGCAATAAAAGGAAAAAGAAAGAATCTGAGCCTCAGGAAAGAGTCCAGAAAAGGCGCAAACCAGATCATTCAGTTCCATCAGCACCAATGATCGACGGAGCATCTGCTCAAGTAAGAGGATGGTCATCTGGAAATGTGTCTAAACGAGATGCATTGCGGTTTTCCCGTGCGGTTATGAAGTTTGGGAATGAGAGCCAAATTGGCTTAATAGTTGAGGAGGTTGGTGGCGCAATTGCAGCAGCTTCACCTGAAGCCCAGGTTGAGCTTTTCAATGCATTGATCGATGGATGCAGAGAAGCAGTGGAAGTGGGGAGTTTGGACCAGAAGGGACCCTTGCTTGATTTTTTTGGTGTCCCTGTGAAGGCTAGTGATCTGGTCAACCGCGTTCAAGAACTTCAGCTCCTGGCAAAGCGCATTGTTCGATATGAAGACCCCATTGGACAGTTCCGTGTCCTGATGTACCTCAAACCTTCAAACTGGTCAAAAGGTTGTGGCTGGAATCAGATTGATGATGCAAGGTTACTTCTTGGAATATACTACCATGGGTTTGGTAATTGGGAAAAGATAAGATTAGATGAAAGGCTTGGCCTTATGAAGAAGATTGCTCCTGTAGAACTTCAGCACCATGAAACATTCTTACCCCGTGCTCCAAATTTGAGAGACCGTGCTAATGCTCTTTTGGAAATGGAACTTGCAGCTCTTGGGGGAAAGAGCGCAAATGCTAAAGTTGGTCGTAAGGCTTCCAAAGAGAGGGAAAATCCTGTCCCTGTTTCTGTGTCTCGCACTGGGGTCAAGAAAGGAAAAGCAGGTTCTTCTAGACTTAATGTCCAAATGAACAAGGACAAACCGCTGAAACCCCAGAGAGTTGAACCTTTAGTGAAAGAAGAGGGTGAAATGTCTGACGATGAAGAAGTATATGAGAAGTTTAAGGAAGAGAAATGGATGGAATGGTGTGAAGAGATGATGACTAGTGAAATTAAAACCCTGAATCGGCTTCATCGTTTGCAGACGACTAGTGCCAATCTTCCAAAGGAGAAGGTATGTCTTAGTATGCGTTGTCTTCTTTGTGCCATTTATTTTCCTTATCATCATTTTCATGCTGGTATTGTTATTCCCATATTTTATTTTCAGGTGCTTTCAAAAATTCGAAATTATTTACAGCTTCTTGGACGAAGGATAGACCAAATTGTTTTGGACAATGAGGAGGAACCATATGGACAGGATAGTATGTTCTTCTTCCTGAATATGATCTATTTGTGTCAGCTCTCTCTTAGTATTGATTTTCTGTCTGTCTTTCCCTCCTGCTTGCATGCATGTAATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chromo DUF4208 Helicase_C SLIDE SNF2_N

Protein Analysis

1665

Amino Acids

189.74

Weight (kDa)

5.68

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chromo PF00385 537 - 591 4.2e-15 Chromo (CHRromatin Organisation MOdifier) domain
ResIII PF04851 627 - 794 8.4e-11 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 631 - 915 1.6e-86 SNF2-related domain
DEAD PF00270 642 - 799 1.6e-07 DEAD/DEAH box helicase
HDA2-3 PF11496 866 - 1090 3.1e-11 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 945 - 1058 2.6e-26 Helicase conserved C-terminal domain
HTH_CHD1_Hrp3 PF23588 1370 - 1451 7.6e-23 ATP-dependent helicase CHD1-2/hrp3 HTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2369, 3107
AarI CACCTGC 1 cut(s) 3056
AatII GACGTC 1 cut(s) 876
Acc36I ACCTGC 3 cut(s) 502, 3056, 4469
Acc65I GGTACC 1 cut(s) 2129
AccB1I GGYRCC 2 cut(s) 227, 2129
AccB7I CCANNNNNTGG 1 cut(s) 3007
AccI GTMKAC 1 cut(s) 988
AccII CGCG 2 cut(s) 3624, 4056
AciI CCGC 9 cut(s) 107, 1099, 1473, 1633, 3095, 3829, 3842, 4054, 4520
AclWI GGATC 8 cut(s) 333, 346, 400, 746, 988, 1329, 1596, 2423
AcoI YGGCCR 3 cut(s) 1111, 2188, 3499
AcvI CACGTG 2 cut(s) 74, 1544
AcyI GRCGYC 1 cut(s) 873
AflII CTTAAG 1 cut(s) 3364
AhdI GACNNNNNGTC 1 cut(s) 4496
AhlI ACTAGT 2 cut(s) 4640, 4685
AjiI CACGTC 1 cut(s) 1567
AjnI CCWGG 8 cut(s) 48, 63, 596, 1520, 3000, 3025, 3927, 4074
AjuI GAANNNNNNNTTGG 4 cut(s) 1953, 1985, 4092, 4124
AloI GAACNNNNNNTCC 4 cut(s) 4056, 4088, 4886, 4918
Alw21I GWGCWC 5 cut(s) 940, 1093, 1287, 3163, 4321
Alw26I GTCTC 6 cut(s) 880, 1132, 2616, 2867, 4326, 4455
Alw44I GTGCAC 2 cut(s) 936, 1089
AlwI GGATC 8 cut(s) 333, 346, 400, 746, 988, 1329, 1596, 2423
AlwNI CAGNNNCTG 4 cut(s) 218, 620, 3656, 4075
ApaI GGGCCC 1 cut(s) 2413
ApaLI GTGCAC 2 cut(s) 936, 1089
ApeKI GCWGC 8 cut(s) 212, 215, 710, 1778, 3656, 3908, 3911, 4367
Asp700I GAANNNNTTC 2 cut(s) 2232, 2592
Asp718I GGTACC 1 cut(s) 2129
AspLEI GCGC 6 cut(s) 2652, 3626, 3729, 3902, 4086, 4388
AspS9I GGNCC 9 cut(s) 68, 188, 478, 588, 2033, 2409, 2410, 3991, 4001
AsuHPI GGTGA 9 cut(s) 616, 1154, 1277, 1519, 1667, 2990, 3286, 3909, 4574
AsuII TTCGAA 2 cut(s) 1171, 4819
AvaII GGWCC 5 cut(s) 68, 188, 588, 3991, 4001
AxyI CCTNAGG 3 cut(s) 97, 330, 3705
BaeGI GKGCMC 4 cut(s) 329, 940, 1093, 2413
BaeI ACNNNNGTAYC 4 cut(s) 1655, 1688, 1929, 1962
BalI TGGCCA 1 cut(s) 3501
BamHI GGATCC 1 cut(s) 338
BanI GGYRCC 2 cut(s) 227, 2129
BanII GRGCYC 2 cut(s) 2413, 3163
BauI CACGAG 2 cut(s) 1075, 1770
BbrPI CACGTG 2 cut(s) 74, 1544
BbsI GAAGAC 5 cut(s) 60, 249, 1538, 4107, 4724
Bbv12I GWGCWC 5 cut(s) 940, 1093, 1287, 3163, 4321
BbvCI CCTCAGC 2 cut(s) 1030, 3378
BbvI GCAGC 8 cut(s) 202, 224, 697, 1765, 3643, 3920, 3923, 4379
BceAI ACGGC 1 cut(s) 3108
BciT130I CCWGG 8 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076
BclI TGATCA 2 cut(s) 829, 1404
BcoDI GTCTC 6 cut(s) 880, 1132, 2616, 2867, 4326, 4455
BcuI ACTAGT 2 cut(s) 4640, 4685
BfmI CTRYAG 4 cut(s) 495, 3196, 3489, 4281
BfrI CTTAAG 1 cut(s) 3364
BfuAI ACCTGC 3 cut(s) 502, 3056, 4469
BglI GCCNNNNNGGC 1 cut(s) 3873
BisI GCNGC 8 cut(s) 213, 216, 711, 1779, 3657, 3909, 3912, 4368
BlpI GCTNAGC 2 cut(s) 2319, 3660
BlsI GCNGC 8 cut(s) 214, 217, 712, 1780, 3658, 3910, 3913, 4369
BmcAI AGTACT 1 cut(s) 2630
Bme1390I CCNGG 8 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076
Bme18I GGWCC 5 cut(s) 68, 188, 588, 3991, 4001
BmeRI GACNNNNNGTC 1 cut(s) 4496
BmgBI CACGTC 1 cut(s) 1567
BmgT120I GGNCC 9 cut(s) 68, 188, 478, 588, 2033, 2409, 2410, 3991, 4001
BmrFI CCNGG 8 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076
BmrI ACTGGG 2 cut(s) 247, 4467
BmuI ACTGGG 2 cut(s) 247, 4467
BpiI GAAGAC 5 cut(s) 60, 249, 1538, 4107, 4724
BplI GAGNNNNNCTC 2 cut(s) 3145, 3177
Bpu10I CCTNAGC 5 cut(s) 638, 1030, 1651, 2103, 3378
Bpu1102I GCTNAGC 2 cut(s) 2319, 3660
Bpu14I TTCGAA 2 cut(s) 1171, 4819
BpuEI CTTGAG 4 cut(s) 1919, 2767, 3476, 3765
Bsa29I ATCGAT 1 cut(s) 3954
BsaAI YACGTR 2 cut(s) 74, 1544
BsaBI GATNNNNATC 2 cut(s) 3126, 3796
BsaHI GRCGYC 1 cut(s) 873
BsaI GGTCTC 1 cut(s) 4326
BsaJI CCNNGG 7 cut(s) 64, 976, 1338, 2764, 3090, 3927, 4213
BsaWI WCCGGW 1 cut(s) 2372
BsaXI ACNNNNNCTCC 4 cut(s) 1334, 1364, 4056, 4086
Bse118I RCCGGY 2 cut(s) 230, 2190
Bse1I ACTGG 4 cut(s) 253, 1438, 4154, 4462
Bse21I CCTNAGG 3 cut(s) 97, 330, 3705
Bse3DI GCAATG 4 cut(s) 1788, 2381, 3156, 3824
Bse8I GATNNNNATC 2 cut(s) 3126, 3796
BseBI CCWGG 8 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076
BseCI ATCGAT 1 cut(s) 3954
BseDI CCNNGG 7 cut(s) 64, 976, 1338, 2764, 3090, 3927, 4213
BseJI GATNNNNATC 2 cut(s) 3126, 3796
BseMI GCAATG 4 cut(s) 1788, 2381, 3156, 3824
BseNI ACTGG 4 cut(s) 253, 1438, 4154, 4462
BseRI GAGGAG 9 cut(s) 85, 280, 1259, 1268, 1271, 2604, 3609, 3902, 4889
BseSI GKGCMC 4 cut(s) 329, 940, 1093, 2413
BseXI GCAGC 8 cut(s) 202, 224, 697, 1765, 3643, 3920, 3923, 4379
BsgI GTGCAG 1 cut(s) 255
Bsh1236I CGCG 2 cut(s) 3624, 4056
BshNI GGYRCC 2 cut(s) 227, 2129
BshVI ATCGAT 1 cut(s) 3954
BsiHKAI GWGCWC 5 cut(s) 940, 1093, 1287, 3163, 4321
BsiSI CCGG 4 cut(s) 231, 1114, 2191, 2373
BslFI GGGAC 8 cut(s) 81, 483, 2792, 3343, 3580, 4010, 4014, 4421
BsmAI GTCTC 6 cut(s) 880, 1132, 2616, 2867, 4326, 4455
BsmBI CGTCTC 2 cut(s) 880, 2867
BsmFI GGGAC 8 cut(s) 81, 483, 2792, 3343, 3580, 4010, 4014, 4421
BsmI GAATGC 2 cut(s) 109, 2020
Bso31I GGTCTC 1 cut(s) 4326
Bsp119I TTCGAA 2 cut(s) 1171, 4819
Bsp120I GGGCCC 1 cut(s) 2409
Bsp1286I GDGCHC 7 cut(s) 329, 940, 1093, 1287, 2413, 3163, 4321
Bsp1407I TGTACA 2 cut(s) 1789, 2325
Bsp1720I GCTNAGC 2 cut(s) 2319, 3660
Bsp19I CCATGG 3 cut(s) 976, 2764, 4213
BspACI CCGC 9 cut(s) 107, 1099, 1473, 1633, 3095, 3829, 3842, 4054, 4520
BspDI ATCGAT 1 cut(s) 3954
BspFNI CGCG 2 cut(s) 3624, 4056
BspHI TCATGA 1 cut(s) 1561
BspMAI CTGCAG 1 cut(s) 499
BspMI ACCTGC 3 cut(s) 502, 3056, 4469
BspPI GGATC 8 cut(s) 333, 346, 400, 746, 988, 1329, 1596, 2423
BspT104I TTCGAA 2 cut(s) 1171, 4819
BspT107I GGYRCC 2 cut(s) 227, 2129
BspTI CTTAAG 1 cut(s) 3364
BspTNI GGTCTC 1 cut(s) 4326
BsrDI GCAATG 4 cut(s) 1788, 2381, 3156, 3824
BsrFI RCCGGY 2 cut(s) 230, 2190
BsrGI TGTACA 2 cut(s) 1789, 2325
BsrI ACTGG 4 cut(s) 253, 1438, 4154, 4462
BssAI RCCGGY 2 cut(s) 230, 2190
BssECI CCNNGG 7 cut(s) 64, 976, 1338, 2764, 3090, 3927, 4213
BssNI GRCGYC 1 cut(s) 873
BssSI CACGAG 2 cut(s) 1075, 1770
BssT1I CCWWGG 3 cut(s) 976, 2764, 4213
Bst2BI CACGAG 2 cut(s) 1075, 1770
Bst2UI CCWGG 8 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076
BstACI GRCGYC 1 cut(s) 873
BstAFI CTTAAG 1 cut(s) 3364
BstAPI GCANNNNNTGC 2 cut(s) 1748, 3656
BstAUI TGTACA 2 cut(s) 1789, 2325
BstBAI YACGTR 2 cut(s) 74, 1544
BstBI TTCGAA 2 cut(s) 1171, 4819
BstC8I GCNNGC 8 cut(s) 615, 2140, 2192, 2505, 3147, 3243, 4979, 4983
BstDSI CCRYGG 5 cut(s) 976, 1338, 2764, 3090, 4213
BstENI CCTNNNNNAGG 1 cut(s) 10
BstFNI CGCG 2 cut(s) 3624, 4056
BstHHI GCGC 6 cut(s) 2652, 3626, 3729, 3902, 4086, 4388
BstMAI GTCTC 6 cut(s) 880, 1132, 2616, 2867, 4326, 4455
BstNI CCWGG 8 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076
BstNSI RCATGY 3 cut(s) 637, 4985, 4989
BstSCI CCNGG 8 cut(s) 48, 63, 596, 1520, 3000, 3025, 3927, 4074
BstSFI CTRYAG 4 cut(s) 495, 3196, 3489, 4281
BstSLI GKGCMC 4 cut(s) 329, 940, 1093, 2413
BstUI CGCG 2 cut(s) 3624, 4056
BstV1I GCAGC 8 cut(s) 202, 224, 697, 1765, 3643, 3920, 3923, 4379
BstV2I GAAGAC 5 cut(s) 60, 249, 1538, 4107, 4724
BstX2I RGATCY 6 cut(s) 338, 392, 980, 1334, 1588, 2428
BstXI CCANNNNNNTGG 3 cut(s) 260, 2771, 4220
BstYI RGATCY 6 cut(s) 338, 392, 980, 1334, 1588, 2428
Bsu15I ATCGAT 1 cut(s) 3954
Bsu36I CCTNAGG 3 cut(s) 97, 330, 3705
BsuTUI ATCGAT 1 cut(s) 3954
BtgI CCRYGG 5 cut(s) 976, 1338, 2764, 3090, 4213
BtrI CACGTC 1 cut(s) 1567
BtsI GCAGTG 5 cut(s) 801, 1432, 2833, 3597, 3978
BveI ACCTGC 3 cut(s) 502, 3056, 4469
Cac8I GCNNGC 8 cut(s) 615, 2140, 2192, 2505, 3147, 3243, 4979, 4983
CaiI CAGNNNCTG 4 cut(s) 218, 620, 3656, 4075
CciI TCATGA 1 cut(s) 1561
CfoI GCGC 6 cut(s) 2652, 3626, 3729, 3902, 4086, 4388
Cfr10I RCCGGY 2 cut(s) 230, 2190
Cfr13I GGNCC 9 cut(s) 68, 188, 478, 588, 2033, 2409, 2410, 3991, 4001
ClaI ATCGAT 1 cut(s) 3954
CsiI ACCWGGT 1 cut(s) 3025
DraI TTTAAA 3 cut(s) 1069, 1491, 3391
DriI GACNNNNNGTC 1 cut(s) 4496
EaeI YGGCCR 3 cut(s) 1111, 2188, 3499
Eam1105I GACNNNNNGTC 1 cut(s) 4496
EciI GGCGGA 1 cut(s) 3110
Ecl136II GAGCTC 1 cut(s) 3161
Eco130I CCWWGG 3 cut(s) 976, 2764, 4213
Eco147I AGGCCT 1 cut(s) 2530
Eco24I GRGCYC 2 cut(s) 2413, 3163
Eco31I GGTCTC 1 cut(s) 4326
Eco32I GATATC 1 cut(s) 3232
Eco47I GGWCC 5 cut(s) 68, 188, 588, 3991, 4001
Eco53kI GAGCTC 1 cut(s) 3161
Eco72I CACGTG 2 cut(s) 74, 1544
Eco81I CCTNAGG 3 cut(s) 97, 330, 3705
EcoICRI GAGCTC 1 cut(s) 3161
EcoNI CCTNNNNNAGG 1 cut(s) 10
EcoO109I RGGNCCY 2 cut(s) 2410, 4001
EcoRI GAATTC 4 cut(s) 84, 1397, 2165, 2592
EcoRII CCWGG 8 cut(s) 48, 63, 596, 1520, 3000, 3025, 3927, 4074
EcoRV GATATC 1 cut(s) 3232
EcoT14I CCWWGG 3 cut(s) 976, 2764, 4213
EcoT22I ATGCAT 4 cut(s) 561, 3826, 3949, 4987
EcoT38I GRGCYC 2 cut(s) 2413, 3163
ErhI CCWWGG 3 cut(s) 976, 2764, 4213
Esp3I CGTCTC 2 cut(s) 880, 2867
FalI AAGNNNNNCTT 4 cut(s) 3597, 3629, 3989, 4021
FaqI GGGAC 8 cut(s) 81, 483, 2792, 3343, 3580, 4010, 4014, 4421
FauNDI CATATG 3 cut(s) 1183, 2224, 4885
FbaI TGATCA 2 cut(s) 829, 1404
FblI GTMKAC 1 cut(s) 988
Fnu4HI GCNGC 8 cut(s) 213, 216, 711, 1779, 3657, 3909, 3912, 4368
FriOI GRGCYC 2 cut(s) 2413, 3163
FseI GGCCGGCC 1 cut(s) 2194
Fsp4HI GCNGC 8 cut(s) 213, 216, 711, 1779, 3657, 3909, 3912, 4368
GlaI GCGC 6 cut(s) 2651, 3625, 3728, 3901, 4085, 4387
GluI GCNGC 8 cut(s) 213, 216, 711, 1779, 3657, 3909, 3912, 4368
HapII CCGG 4 cut(s) 231, 1114, 2191, 2373
HhaI GCGC 6 cut(s) 2652, 3626, 3729, 3902, 4086, 4388
Hin1I GRCGYC 1 cut(s) 873
Hin6I GCGC 6 cut(s) 2650, 3624, 3727, 3900, 4084, 4386
HinP1I GCGC 6 cut(s) 2650, 3624, 3727, 3900, 4084, 4386
HincII GTYRAC 5 cut(s) 317, 989, 2217, 3190, 4051
HindII GTYRAC 5 cut(s) 317, 989, 2217, 3190, 4051
HindIII AAGCTT 3 cut(s) 1844, 1880, 2852
HpaII CCGG 4 cut(s) 231, 1114, 2191, 2373
HphI GGTGA 9 cut(s) 616, 1154, 1277, 1519, 1667, 2990, 3286, 3909, 4574
Hpy166II GTNNAC 9 cut(s) 317, 591, 938, 989, 1091, 2217, 2328, 3190, 4051
Hpy8I GTNNAC 9 cut(s) 317, 591, 938, 989, 1091, 2217, 2328, 3190, 4051
Hpy99I CGWCG 1 cut(s) 3770
HpyCH4IV ACGT 6 cut(s) 73, 873, 1543, 1566, 1725, 2995
HpySE526I ACGT 6 cut(s) 73, 873, 1543, 1566, 1725, 2995
Hsp92I GRCGYC 1 cut(s) 873
HspAI GCGC 6 cut(s) 2650, 3624, 3727, 3900, 4084, 4386
KflI GGGWCCC 1 cut(s) 4001
KpnI GGTACC 1 cut(s) 2133
KroI GCCGGC 1 cut(s) 2190
KroNI GCCGGC 1 cut(s) 2192
Ksp22I TGATCA 2 cut(s) 829, 1404
Lsp1109I GCAGC 8 cut(s) 202, 224, 697, 1765, 3643, 3920, 3923, 4379
MabI ACCWGGT 1 cut(s) 3025
MaeII ACGT 6 cut(s) 73, 873, 1543, 1566, 1725, 2995
MaeIII GTNAC 9 cut(s) 548, 622, 1049, 1265, 1358, 1422, 2996, 3205, 4191
MfeI CAATTG 1 cut(s) 3903
MflI RGATCY 6 cut(s) 338, 392, 980, 1334, 1588, 2428
MhlI GDGCHC 7 cut(s) 329, 940, 1093, 1287, 2413, 3163, 4321
MlsI TGGCCA 1 cut(s) 3501
MluNI TGGCCA 1 cut(s) 3501
MlyI GAGTC 7 cut(s) 545, 1034, 1034, 1364, 2774, 3577, 3723
MmeI TCCRAC 5 cut(s) 370, 1837, 1907, 2898, 3554
Mox20I TGGCCA 1 cut(s) 3501
Mph1103I ATGCAT 4 cut(s) 561, 3826, 3949, 4987
MroNI GCCGGC 1 cut(s) 2190
MroXI GAANNNNTTC 2 cut(s) 2232, 2592
MscI TGGCCA 1 cut(s) 3501
MslI CAYNNNNRTG 3 cut(s) 2769, 3018, 4767
Msp20I TGGCCA 1 cut(s) 3501
MspA1I CMGCKG 3 cut(s) 215, 2323, 4522
MspCI CTTAAG 1 cut(s) 3364
MspI CCGG 4 cut(s) 231, 1114, 2191, 2373
MspR9I CCNGG 8 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076
MunI CAATTG 1 cut(s) 3903
Mva1269I GAATGC 2 cut(s) 109, 2020
MvaI CCWGG 8 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076
MvnI CGCG 2 cut(s) 3624, 4056
NaeI GCCGGC 1 cut(s) 2192
NcoI CCATGG 3 cut(s) 976, 2764, 4213
NdeI CATATG 3 cut(s) 1183, 2224, 4885
NgoMIV GCCGGC 1 cut(s) 2190
NmeAIII GCCGAG 2 cut(s) 2168, 2956
NmuCI GTSAC 7 cut(s) 548, 622, 1049, 1265, 1358, 1422, 2996
NsiI ATGCAT 4 cut(s) 561, 3826, 3949, 4987
NspI RCATGY 3 cut(s) 637, 4985, 4989
NspV TTCGAA 2 cut(s) 1171, 4819
PaeI GCATGC 1 cut(s) 4985
PagI TCATGA 1 cut(s) 1561
PaqCI CACCTGC 1 cut(s) 3056
PceI AGGCCT 1 cut(s) 2530
PcsI WCGNNNNNNNCGW 1 cut(s) 4669
PctI GAATGC 2 cut(s) 109, 2020
PdiI GCCGGC 1 cut(s) 2192
PdmI GAANNNNTTC 2 cut(s) 2232, 2592
PflMI CCANNNNNTGG 1 cut(s) 3007
PfoI TCCNGGA 1 cut(s) 48
PkrI GCNGC 8 cut(s) 214, 217, 712, 1780, 3658, 3910, 3913, 4369
PleI GAGTC 7 cut(s) 545, 1033, 1034, 1364, 2774, 3576, 3722
PmaCI CACGTG 2 cut(s) 74, 1544
PmlI CACGTG 2 cut(s) 74, 1544
PpsI GAGTC 7 cut(s) 545, 1033, 1034, 1364, 2774, 3576, 3722
Ppu21I YACGTR 2 cut(s) 74, 1544
PpuMI RGGWCCY 1 cut(s) 4001
PsiI TTATAA 2 cut(s) 2369, 3107
Psp124BI GAGCTC 1 cut(s) 3163
Psp5II RGGWCCY 1 cut(s) 4001
Psp6I CCWGG 8 cut(s) 48, 63, 596, 1520, 3000, 3025, 3927, 4074
PspCI CACGTG 2 cut(s) 74, 1544
PspGI CCWGG 8 cut(s) 48, 63, 596, 1520, 3000, 3025, 3927, 4074
PspOMI GGGCCC 1 cut(s) 2409
PspPI GGNCC 9 cut(s) 68, 188, 478, 588, 2033, 2409, 2410, 3991, 4001
PspPPI RGGWCCY 1 cut(s) 4001
PstI CTGCAG 1 cut(s) 499
PstNI CAGNNNCTG 4 cut(s) 218, 620, 3656, 4075
PsuI RGATCY 6 cut(s) 338, 392, 980, 1334, 1588, 2428
PvuII CAGCTG 2 cut(s) 215, 2323
RigI GGCCGGCC 1 cut(s) 2194
RseI CAYNNNNRTG 3 cut(s) 2769, 3018, 4767
SacI GAGCTC 1 cut(s) 3163
SalI GTCGAC 1 cut(s) 987
SatI GCNGC 8 cut(s) 213, 216, 711, 1779, 3657, 3909, 3912, 4368
Sau96I GGNCC 9 cut(s) 68, 188, 478, 588, 2033, 2409, 2410, 3991, 4001
ScaI AGTACT 1 cut(s) 2630
SchI GAGTC 7 cut(s) 545, 1034, 1034, 1364, 2774, 3577, 3723
ScrFI CCNGG 8 cut(s) 50, 65, 598, 1522, 3002, 3027, 3929, 4076
SduI GDGCHC 7 cut(s) 329, 940, 1093, 1287, 2413, 3163, 4321
SexAI ACCWGGT 1 cut(s) 3025
SfcI CTRYAG 4 cut(s) 495, 3196, 3489, 4281
SfuI TTCGAA 2 cut(s) 1171, 4819
SinI GGWCC 5 cut(s) 68, 188, 588, 3991, 4001
SmiMI CAYNNNNRTG 3 cut(s) 2769, 3018, 4767
SmlI CTYRAG 5 cut(s) 1898, 2782, 3364, 3455, 3780
SmoI CTYRAG 5 cut(s) 1898, 2782, 3364, 3455, 3780
SpeI ACTAGT 2 cut(s) 4640, 4685
SphI GCATGC 1 cut(s) 4985
SseBI AGGCCT 1 cut(s) 2530
SsiI CCGC 9 cut(s) 107, 1099, 1473, 1633, 3095, 3829, 3842, 4054, 4520
SspI AATATT 4 cut(s) 521, 703, 2707, 3111
SstI GAGCTC 1 cut(s) 3163
StuI AGGCCT 1 cut(s) 2530
StyD4I CCNGG 8 cut(s) 48, 63, 596, 1520, 3000, 3025, 3927, 4074
StyI CCWWGG 3 cut(s) 976, 2764, 4213
TaiI ACGT 6 cut(s) 76, 876, 1546, 1569, 1728, 2998
TaqI TCGA 9 cut(s) 988, 1171, 1614, 1806, 1994, 3765, 3954, 4093, 4819
TatI WGTACW 5 cut(s) 375, 1789, 2325, 2628, 2978
TseFI GTSAC 7 cut(s) 548, 622, 1049, 1265, 1358, 1422, 2996
TseI GCWGC 8 cut(s) 212, 215, 710, 1778, 3656, 3908, 3911, 4367
Tsp45I GTSAC 7 cut(s) 548, 622, 1049, 1265, 1358, 1422, 2996
TspGWI ACGGA 3 cut(s) 1355, 3783, 4109
Van91I CCANNNNNTGG 1 cut(s) 3007
Vha464I CTTAAG 1 cut(s) 3364
VneI GTGCAC 2 cut(s) 936, 1089
VpaK11BI GGWCC 5 cut(s) 68, 188, 588, 3991, 4001
XagI CCTNNNNNAGG 1 cut(s) 10
XbaI TCTAGA 1 cut(s) 4487
XceI RCATGY 3 cut(s) 637, 4985, 4989
XcmI CCANNNNNNNNNTGG 1 cut(s) 2771
XmiI GTMKAC 1 cut(s) 988
XmnI GAANNNNTTC 2 cut(s) 2232, 2592
ZraI GACGTC 1 cut(s) 874
ZrmI AGTACT 1 cut(s) 2630
Zsp2I ATGCAT 4 cut(s) 561, 3826, 3949, 4987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.