MD11G1194800.v1.1

chromodomain-helicase-DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007957
Physical Location & Seq
Reverse (-)
27912034 .. 27932533
20500 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1194800.v1.1.491

Sequence Viewer

Length: 5244 bp
ATGGCTTTTTTTAGGAACTATTCTAAAGACACTGTTTCACGAAGTGTCCTCGAAGAAAAGACCCAGGGAAAAAGTGTTGAAAGAATTCACAGTTCTACAGGGAATGATGATGTGGATGTAAAATCTTGTGAAAAGGAATTTGATATCAACATGGATGTGCAGTATCAAAGTGAAGGTGAACATGATGATGCTAGTAGGTTGCACAATGAGGTAGCAACAGATGAGGGTATGGGTACAAGAGTTTCCAATTTGCCCTCTTCTGGGAGGAGGACAACTGTGGCTGGAAAGTGGGGTTCGACCTTTTGGAAGGACTGTCAGCCTATGCACTCACAGGGCGGGTCTGATTCTGGTCAGGAAACTAAAAGTGGGTCGGACCACAGAAATGCTGTTGGATCTGAAGATAATTCTTCAGATGTCGGGGAAGATAGAATAGATTTTGAAGATGATGGCAGGCCAAAGGTAAGCAAGGGACAACGGGGGCATTCTGACATCCCTGCAGATGAAATGTTATCTGATGAATATTATGAGCAGGATGGGGAAGAACAGAGTGAATCGATGCACTATAGAGGGTTTCATCATTCCGTTGGGTCAAATTCTAGGCCACAATTAAAGCCTAATTCAGTCAGTAACCATGCACATCATAGAACTTCTAGAAATGATATTGAAGATTTTGAAGATGGTGATGATGCCAACGAAGATCTTGATGCTGCTGATGCTGATTATGAAGAAGACGAAGATGAAGATGATCCTGATGATGCAGATTTTGAACCTGACTATGGTGTTGGGAGTGGTCGCACTGCACAGAAGGATAAAAATTGGAATGGTGAAGATTCTGATGAAGACAATGATAGTGATGATGAGCTTGATGTTTCAGATGAAGATGATTCTTATTATGGAAAGAAACCTAAGAGTAGGCATCAAGGTGATGCTAAATCGACAAGGGAACGCAAATCATATCATTCGTCTAGTCGCCAAAGAAGAGGGAAGTCATCTTTTGATGATGATGAGTCCTCAGCGGAGGACTCTGAGAGTGATGGTGACGAGGATTTTAAAGGTATCAAAAGGAAAGGTGTACATACCCGCAAAAGTAACGGCCGAAGAAATGTTCTTGGACAAAATGGTGAGGTGCGAACTTCTACGAGGTCTGTTAGGAAGGTCTCATATGTTGAAAGTGATGGAAGCGATGAAGTGGAAGAAGGAAAGAAAAAGAAGTCCCAGAAGGAGGAGATTGAAGAAGAGGATGGTGACTATATTGAAAAGGTGCTCTGGCATCAGCCTAAAGGCATGGCTGAAGATGCTCTCAGGAACAATAGATCAACGGATCCTATCCTATTAAGTCACTTGTTTGATTCTGAGCCAGATTGGAATCATATGGAGTTCTTGATTAAATGGAAAGGGCAGTCCCATTTGCACTGTCAGTGGAAATCAATTTCCGAGCTACAAAATCTTAGCGGTTTTAAGAAGGTTTTAAATTACACTAAAAAGGTGATGGAAGATGCCAGGTACAGGAACACAATATCACGTGAGGAGATTGAGGTTCATGATGTGAGCAAGGAAATGGACTTGGATCTTATCAAGCAGAATAGTCAGGTGGAGAGAATAATTGCAGACCGCATCAGGCAAGATAGCTCTGGTGATGTGGGTCCGGAGTATCTAGTCAAGTGGCAAGGGTTGTCTTATGCTGAAGCAACATGGGAGAAGGATGTAGATATTGGGTTTGCACAAGATGCTATCGATGAGTTCAAGGCTCGTGAAGCTGCAATGGCTGTACAAGGGAAAATGGTGGATCTCCAGCGCAAGAGGAGCAAAGGAAGTCTACGGAAGCTCGAGGAACAGCCGGAATGGTTGAAGGGAGGAAAGCTTCGTGATTATCAGCTTGAGGCTCTTGCATGTTGCAGTTGGAGGAATGATACCAATGTCATTTTAGCTGATGAAATGGGTCTTGGTAAAACTGTTCAATCAGTTTCAATGCTTGGTTTTTTACAGAATGCTCAACAGATCAATGGGCCGTTTCTTGTTGTTGTACCATTATCCACTTTGTCTAACTGGGCCAAAGAATTTAGGAAGTGGCTCCCTGATATGAATATTATTGTGTATGTTGGTACTCGTGCAAGCCGAGAGGTTTGTCAACAGTATGAATTTAATAATGATAAAATAGTAGGCCGGCCTATAAAATTTAACGCACTATTGACGACATATGAGGTGGTTTTAAAGGATAAGGCTGTTCTTTCCAAGATCAAGTGGAATTATCTAATGGTTGATGAAGCTCATAGATTAAAGAACAGTGAGGCACAGTTGTACACAACCCTCTTGGAATTTAGCACGAAGAACAAGTTGCTTATAACTGGTACTCCCTTGCAGAACAGCGTAGAGGAGTTATGTTTTGTTTCTGGCTGCCCTTATCTGGCTCTGCTTCACTTCCTTGACCCTGATAAGTTCAAGAATAAAGATGATTTTGTTCAGAATTACAAGAATCTCAGTTCATTCAATGAGATTGAGCTTGCTAATCTGCATATGGAATTGAGGCCTCACATTCTTCGAAGAGTTATCAAGGATGTGGAAAAGTCTTTACCTCCTAAAATTGAACGAATTCTTAGGGTTGAAATGTCTCCTCTTCAGAAACAGTACTATAAATGGATTTTGGAACGCAACTTTCATAACTTGAACAAAGGAGTTCGTGGGAATCAGGTTTCGTTGTTGAATATTGTGGTAGAGTTGAAGAAATGTTGTAATCATCCCTTTCTGTTTGAAAGTGCTGACCATGGTTATGGTGGGGACTCAAGTATTAAAGATGGCAGTAAATTGGAGAGGATTATTTTAAGCAGTGGAAAATTGGTTATACTTGATAAGCTTCTTATGAAATTGCATGAAACAAAGCACCGTGTTCTCATATTTTCCCAGATGGTAAGAATGTTGGACATACTGGCGGAGTATATGTCAATTAGAGGTTTTCAATTTCAGCGGCTTGATGGGAGTACAAAGGCCGATTTACGCCACCAAGCAATGGAACATTTTAATGCGCCTGGTAGTGAAGATTTTTGCTTCCTCCTTTCAACTCGAGCCGGTGGCCTTGGTATCAACCTTGCCACAGCGGATACAGTGATAATATTCGACTCAGATTGGAATCCCCAAAATGACCTTCAGGCAATGAGCAGAGCGCACAGAATAGGGCAACAAGAAGTTGTTAACATCTATAGATTTGTCACAAGCAAAAGTGTCGAGGAAGATATTCTGGAGAGAGCTAAGAAAAAGATGGTGCTTGACCATTTGGTGATTCAAAAATTAAATGCAGAGGGTAGGTTGGAGAAGAAAGAATCTAAGAAAGGGACTTTATTTGACAAAAATGAGCTGTCTGCTATTCTACGATTTGGGGCTGAGGAACTTTTCAAGGAAGACAAAAATGATGAAGAGAGCAAGAAAGGACTCTTGAGTATGGATATTGATGAGATTCTTGAAAGAGCAGAAAAGGTTGAAGAGAAGGAAACTGCAGAGGAAGGGAATGAATTGTTGAGTGCTTTTAAGGTTGCAAATTTTGGTACTGGTGAAGATGATGGTAGTTTTTGGAGTCGTTGGATAAAGCCAGAAGCAGTATCCCAAGCTGAAGAAGCGCTTGCTCCTCGTGCTACAAGGAATACTAAGAGCTATGCAGAAGCCGATCAGCCTGATAGAAGTAATAAAAGAAAAAAGAAAGAATCTGAGCCTCAGGAAAGAGTCCAGAAAAGGCGGAAATCAGATTATACAGTTACCTCTGCTCCAATGATTGGCGGAGCATCTGCTCAAGTTAGGAGATGGTCTTCTGGGAACTTATCTAAAAGAGACGCATTGCGATTTTCTCGTGCTGTTATGAAATTTGGGAATGAGAGCCAAATTGCCTCGATAGTTGGGGAGGTTGGTGGTGCCGTTGCAGCAGCTTCAACTGAAGCACAGGTTGAGCTTTTTAATGCATTGATTGATGGCTGCACAGAAGCAGTGGAAGTGGGGAGTCTGGACTCGAAGGGGCCCATGCTGGATTTTTTTGGTGTCCCTGTCAAAGCTGGTGATCTGCTCAATCGTGTTCGTGAACTTCAGCACCTGGCAAAACGCATTAGTCGATATGACGATCCTATTGCTCAGTTTCGTGTCTTGATGTACCTCAAACCTTCAAATTGGTCGAAAGGTTGTGGTTGGAATCAAATTGATGACGCAAGATTGCTTATTGGAATATACTACCATGGCTTTGGAAATTGGGAAAAGATACGTTTAGATGAAAGGCTTGGCCTTACAAAGAAAATTGCTCCAGTGGAACTTCAACACCATGAAACATTTTTACCCCGTGCTCCAAATTTAAGAGATCGTGCTAACGCTCTTTTGGAAATGGAAATTGCAGCCCTTGGTGGAAAGAATGCAAATGCTAAAGTGGGTCGTAAGTCTTCAAAGGATAGGGAAACTACTGTCAATGTTTCTTTGGCTCGCAGTGGGCTAAAGAAAGGAAAAACTGGTTCTTCTAGAGTTAATGTTCAAATGAACAAGGATAGACCACTGAAACCCCAGAAAGTTGAAGCTTTAGTGAAAGAGGAGGGTGAAATGTCGGATGATGATGAAGTGTATGAGAAGTTCAAGGAGGAGAAATGGATGGAATGGTGTGAAGAGATGATGAAGGATGAAACTAAAACCTTGAAACGTCTTCAACGGTTGCAGACGACTAGTGCCAATCTTCCAAAGGAAAAGGTGCTTTCCAAGATTCGAAGTTATTTACAACTTCTTGGACGAACGATAGATCAAATTGTTTTAGAAAATGAGGAGGAACCGTATGGACAAGATAGGATGACCATGCGCTTGTGGAAATATGTTTCTACCTTTTCAAATCTGTCCGGGGAGAGACTTCATCAAATATATTCAAAACTTAAACAGCACCAGGATGTGGAGGCTGGCCCTTCCCACATGAACGACTCCGCTTCTGGTCCCTTCGGCAGAGACAGCGACCCTTCTCCCTTCTCCTTCCCTTCGGACAGGCAAAGAGGTCATGATACAGCCAAGTTTGAAGCATGGAAACGGAGAAGGAGAGGGGAAGCTGATAGTAATTTCCAAGTTCAGGCCCCATCTCAAAGAAATATGAATAATGGTACCCGGTTAACTGATCCAAGCTCATTAGGGATTCTTGGTGCAAGACCATCGGAAAATAAACGCACTGTGAATGAAAGGCCATACAGGACGCGACAGGCAGGTTTACCAACAAAACAAGGTTTTACATCACGTATTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chromo DUF4208 Helicase_C SNF2_N

Protein Analysis

1748

Amino Acids

198.76

Weight (kDa)

5.79

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chromo PF00385 530 - 584 2.6e-14 Chromo (CHRromatin Organisation MOdifier) domain
ResIII PF04851 620 - 785 3.3e-10 Type III restriction enzyme, res subunit
DEAD PF00270 623 - 790 3.2e-08 DEAD/DEAH box helicase
SNF2-rel_dom PF00176 624 - 914 2.2e-77 SNF2-related domain
HDA2-3 PF11496 865 - 1089 2.5e-11 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 944 - 1057 6.4e-26 Helicase conserved C-terminal domain
HTH_CHD1_Hrp3 PF23588 1368 - 1450 2e-23 ATP-dependent helicase CHD1-2/hrp3 HTH domain
CHD1-like_C PF13907 1533 - 1627 3.2e-16 Chromodomain-helicase-DNA-binding protein 1-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2342
Acc36I ACCTGC 1 cut(s) 5192
Acc65I GGTACC 1 cut(s) 5102
AccB1I GGYRCC 2 cut(s) 3896, 5102
AccB7I CCANNNNNTGG 3 cut(s) 3004, 3761, 4900
AccI GTMKAC 1 cut(s) 1816
AccII CGCG 1 cut(s) 5194
AccIII TCCGGA 1 cut(s) 1645
AclWI GGATC 8 cut(s) 400, 740, 1316, 1329, 1575, 1794, 4094, 5111
AcoI YGGCCR 1 cut(s) 1093
AcuI CTGAAG 9 cut(s) 393, 417, 1311, 1704, 2600, 3125, 3621, 3939, 4049
AcvI CACGTG 1 cut(s) 1523
AdeI CACNNNGTG 1 cut(s) 44
AfeI AGCGCT 1 cut(s) 3609
AhlI ACTAGT 1 cut(s) 4682
AjnI CCWGG 5 cut(s) 63, 1499, 3022, 4071, 4893
AjuI GAANNNNNNNTTGG 4 cut(s) 1926, 1958, 3123, 3155
Alw21I GWGCWC 2 cut(s) 1266, 4318
Alw26I GTCTC 5 cut(s) 1162, 2613, 3810, 4852, 4947
AlwI GGATC 8 cut(s) 400, 740, 1316, 1329, 1575, 1794, 4094, 5111
AlwNI CAGNNNCTG 1 cut(s) 4072
Ama87I CYCGRG 2 cut(s) 1826, 3057
Aor13HI TCCGGA 1 cut(s) 1645
Aor51HI AGCGCT 1 cut(s) 3609
ApaI GGGCCC 1 cut(s) 4002
ApeKI GCWGC 7 cut(s) 707, 1757, 2394, 3905, 3908, 3957, 4364
Asp700I GAANNNNTTC 3 cut(s) 84, 2589, 3228
Asp718I GGTACC 1 cut(s) 5102
AspLEI GCGC 5 cut(s) 1797, 3022, 3160, 3610, 4815
AspS9I GGNCC 9 cut(s) 373, 1643, 2006, 2049, 3998, 3999, 4910, 4940, 5074
AsuC2I CCSGG 2 cut(s) 4852, 5107
AsuII TTCGAA 2 cut(s) 2539, 4723
AvaI CYCGRG 2 cut(s) 1826, 3057
AvaII GGWCC 3 cut(s) 373, 1643, 4940
AxyI CCTNAGG 1 cut(s) 3702
BaeGI GKGCMC 1 cut(s) 4002
BaeI ACNNNNGTAYC 4 cut(s) 225, 258, 1902, 1935
BamHI GGATCC 1 cut(s) 1321
BanI GGYRCC 2 cut(s) 3896, 5102
BanII GRGCYC 1 cut(s) 4002
BauI CACGAG 4 cut(s) 1749, 2106, 3618, 3834
BbrPI CACGTG 1 cut(s) 1523
BbsI GAAGAC 6 cut(s) 735, 846, 3399, 3786, 4401, 4655
Bbv12I GWGCWC 2 cut(s) 1266, 4318
BbvCI CCTCAGC 2 cut(s) 1012, 3375
BbvI GCAGC 7 cut(s) 694, 1744, 2381, 3917, 3920, 3944, 4376
BceAI ACGGC 3 cut(s) 1108, 1993, 3884
BcgI CGANNNNNNTGC 6 cut(s) 3199, 3233, 4088, 4122, 5133, 5167
BciT130I CCWGG 5 cut(s) 65, 1501, 3024, 4073, 4895
BciVI GTATCC 2 cut(s) 3088, 3601
BcnI CCSGG 2 cut(s) 4852, 5107
BcoDI GTCTC 5 cut(s) 1162, 2613, 3810, 4852, 4947
BcuI ACTAGT 1 cut(s) 4682
BfaI CTAG 7 cut(s) 192, 597, 651, 966, 1655, 4485, 4683
BfmI CTRYAG 5 cut(s) 96, 495, 562, 3193, 3486
BfoI RGCGCY 1 cut(s) 3611
BfuAI ACCTGC 1 cut(s) 5192
BfuI GTATCC 2 cut(s) 3088, 3601
BglII AGATCT 1 cut(s) 697
BisI GCNGC 8 cut(s) 708, 1758, 2395, 2963, 3906, 3909, 3958, 4365
BlsI GCNGC 8 cut(s) 709, 1759, 2396, 2964, 3907, 3910, 3959, 4366
BmcAI AGTACT 1 cut(s) 2627
Bme1390I CCNGG 7 cut(s) 65, 1501, 3024, 4073, 4852, 4895, 5107
Bme18I GGWCC 3 cut(s) 373, 1643, 4940
BmeT110I CYCGRG 2 cut(s) 1826, 3057
BmgT120I GGNCC 9 cut(s) 373, 1643, 2006, 2049, 3998, 3999, 4910, 4940, 5074
BmrFI CCNGG 7 cut(s) 65, 1501, 3024, 4073, 4852, 4895, 5107
BmrI ACTGGG 1 cut(s) 2056
BmuI ACTGGG 1 cut(s) 2056
BpiI GAAGAC 6 cut(s) 735, 846, 3399, 3786, 4401, 4655
BpmI CTGGAG 3 cut(s) 1775, 3254, 4260
Bpu10I CCTNAGC 2 cut(s) 1012, 3375
Bpu14I TTCGAA 2 cut(s) 2539, 4723
BpuEI CTTGAG 4 cut(s) 1898, 2764, 3448, 3762
BpuMI CCSGG 2 cut(s) 4852, 5107
Bsa29I ATCGAT 2 cut(s) 554, 1734
BsaAI YACGTR 2 cut(s) 1523, 5234
BsaBI GATNNNNATC 2 cut(s) 1365, 3123
BsaI GGTCTC 1 cut(s) 1162
BsaJI CCNNGG 7 cut(s) 63, 64, 2761, 3070, 4210, 4369, 4851
BsaWI WCCGGW 1 cut(s) 1645
BsaXI ACNNNNNCTCC 8 cut(s) 2335, 2365, 2921, 2951, 3736, 3766, 4772, 4802
Bse118I RCCGGY 2 cut(s) 2163, 3062
Bse1I ACTGG 6 cut(s) 2051, 2350, 2928, 3544, 4277, 4480
Bse21I CCTNAGG 1 cut(s) 3702
Bse3DI GCAATG 4 cut(s) 1767, 3009, 3153, 3821
Bse8I GATNNNNATC 2 cut(s) 1365, 3123
BseAI TCCGGA 1 cut(s) 1645
BseBI CCWGG 5 cut(s) 65, 1501, 3024, 4073, 4895
BseCI ATCGAT 2 cut(s) 554, 1734
BseDI CCNNGG 7 cut(s) 63, 64, 2761, 3070, 4210, 4369, 4851
BseJI GATNNNNATC 2 cut(s) 1365, 3123
BseMI GCAATG 4 cut(s) 1767, 3009, 3153, 3821
BseNI ACTGG 6 cut(s) 2051, 2350, 2928, 3544, 4277, 4480
BseSI GKGCMC 1 cut(s) 4002
BseX3I CGGCCG 1 cut(s) 1093
BseXI GCAGC 7 cut(s) 694, 1744, 2381, 3917, 3920, 3944, 4376
BsgI GTGCAG 3 cut(s) 179, 783, 3943
Bsh1236I CGCG 1 cut(s) 5194
Bsh1285I CGRYCG 1 cut(s) 1096
BshNI GGYRCC 2 cut(s) 3896, 5102
BshVI ATCGAT 2 cut(s) 554, 1734
BsiEI CGRYCG 1 cut(s) 1096
BsiHKAI GWGCWC 2 cut(s) 1266, 4318
BsiHKCI CYCGRG 2 cut(s) 1826, 3057
BsiSI CCGG 6 cut(s) 1646, 1838, 2164, 3063, 4851, 5107
BslFI GGGAC 7 cut(s) 483, 1198, 1387, 2789, 3340, 4007, 4926
BsmAI GTCTC 5 cut(s) 1162, 2613, 3810, 4852, 4947
BsmBI CGTCTC 1 cut(s) 3810
BsmFI GGGAC 7 cut(s) 483, 1198, 1387, 2789, 3340, 4007, 4926
BsmI GAATGC 3 cut(s) 481, 1993, 4387
Bso31I GGTCTC 1 cut(s) 1162
BsoBI CYCGRG 2 cut(s) 1826, 3057
Bsp119I TTCGAA 2 cut(s) 2539, 4723
Bsp120I GGGCCC 1 cut(s) 3998
Bsp1286I GDGCHC 3 cut(s) 1266, 4002, 4318
Bsp13I TCCGGA 1 cut(s) 1645
Bsp1407I TGTACA 3 cut(s) 1072, 1768, 2298
Bsp19I CCATGG 2 cut(s) 2761, 4210
BspDI ATCGAT 2 cut(s) 554, 1734
BspEI TCCGGA 1 cut(s) 1645
BspFNI CGCG 1 cut(s) 5194
BspHI TCATGA 2 cut(s) 1540, 5002
BspMAI CTGCAG 2 cut(s) 499, 3490
BspMI ACCTGC 1 cut(s) 5192
BspPI GGATC 8 cut(s) 400, 740, 1316, 1329, 1575, 1794, 4094, 5111
BspT104I TTCGAA 2 cut(s) 2539, 4723
BspT107I GGYRCC 2 cut(s) 3896, 5102
BspTNI GGTCTC 1 cut(s) 1162
BsrDI GCAATG 4 cut(s) 1767, 3009, 3153, 3821
BsrFI RCCGGY 2 cut(s) 2163, 3062
BsrGI TGTACA 3 cut(s) 1072, 1768, 2298
BsrI ACTGG 6 cut(s) 2051, 2350, 2928, 3544, 4277, 4480
BssAI RCCGGY 2 cut(s) 2163, 3062
BssECI CCNNGG 7 cut(s) 63, 64, 2761, 3070, 4210, 4369, 4851
BssSI CACGAG 4 cut(s) 1749, 2106, 3618, 3834
BssT1I CCWWGG 4 cut(s) 2761, 3070, 4210, 4369
Bst2BI CACGAG 4 cut(s) 1749, 2106, 3618, 3834
Bst2UI CCWGG 5 cut(s) 65, 1501, 3024, 4073, 4895
Bst6I CTCTTC 8 cut(s) 262, 973, 1230, 2536, 2619, 3402, 3468, 4620
BstAPI GCANNNNNTGC 1 cut(s) 1727
BstAUI TGTACA 3 cut(s) 1072, 1768, 2298
BstBAI YACGTR 2 cut(s) 1523, 5234
BstBI TTCGAA 2 cut(s) 2539, 4723
BstC8I GCNNGC 7 cut(s) 452, 2113, 2165, 2502, 3612, 4450, 4909
BstDSI CCRYGG 2 cut(s) 2761, 4210
BstFNI CGCG 1 cut(s) 5194
BstH2I RGCGCY 1 cut(s) 3611
BstHHI GCGC 5 cut(s) 1797, 3022, 3160, 3610, 4815
BstMAI GTCTC 5 cut(s) 1162, 2613, 3810, 4852, 4947
BstMCI CGRYCG 1 cut(s) 1096
BstNI CCWGG 5 cut(s) 65, 1501, 3024, 4073, 4895
BstNSI RCATGY 1 cut(s) 1893
BstSCI CCNGG 7 cut(s) 63, 1499, 3022, 4071, 4850, 4893, 5105
BstSFI CTRYAG 5 cut(s) 96, 495, 562, 3193, 3486
BstSLI GKGCMC 1 cut(s) 4002
BstUI CGCG 1 cut(s) 5194
BstV1I GCAGC 7 cut(s) 694, 1744, 2381, 3917, 3920, 3944, 4376
BstV2I GAAGAC 6 cut(s) 735, 846, 3399, 3786, 4401, 4655
BstX2I RGATCY 5 cut(s) 392, 697, 1321, 1567, 1786
BstXI CCANNNNNNTGG 2 cut(s) 2768, 4217
BstYI RGATCY 5 cut(s) 392, 697, 1321, 1567, 1786
BstZI CGGCCG 1 cut(s) 1093
Bsu15I ATCGAT 2 cut(s) 554, 1734
Bsu36I CCTNAGG 1 cut(s) 3702
BsuI GTATCC 2 cut(s) 3088, 3601
BsuTUI ATCGAT 2 cut(s) 554, 1734
BtgI CCRYGG 2 cut(s) 2761, 4210
BtgZI GCGATG 1 cut(s) 1197
BtsI GCAGTG 4 cut(s) 795, 2830, 3975, 4459
BveI ACCTGC 1 cut(s) 5192
Cac8I GCNNGC 7 cut(s) 452, 2113, 2165, 2502, 3612, 4450, 4909
CaiI CAGNNNCTG 1 cut(s) 4072
CciI TCATGA 2 cut(s) 1540, 5002
CfoI GCGC 5 cut(s) 1797, 3022, 3160, 3610, 4815
Cfr10I RCCGGY 2 cut(s) 2163, 3062
Cfr13I GGNCC 9 cut(s) 373, 1643, 2006, 2049, 3998, 3999, 4910, 4940, 5074
ClaI ATCGAT 2 cut(s) 554, 1734
CseI GACGC 3 cut(s) 3827, 4190, 5200
DraI TTTAAA 3 cut(s) 1051, 1470, 2211
DraIII CACNNNGTG 1 cut(s) 44
EaeI YGGCCR 1 cut(s) 1093
EagI CGGCCG 1 cut(s) 1093
Eam1104I CTCTTC 8 cut(s) 262, 973, 1230, 2536, 2619, 3402, 3468, 4620
EarI CTCTTC 8 cut(s) 262, 973, 1230, 2536, 2619, 3402, 3468, 4620
EciI GGCGGA 3 cut(s) 2942, 3739, 3780
EclXI CGGCCG 1 cut(s) 1093
Eco130I CCWWGG 4 cut(s) 2761, 3070, 4210, 4369
Eco147I AGGCCT 1 cut(s) 2527
Eco24I GRGCYC 1 cut(s) 4002
Eco31I GGTCTC 1 cut(s) 1162
Eco32I GATATC 1 cut(s) 145
Eco47I GGWCC 3 cut(s) 373, 1643, 4940
Eco47III AGCGCT 1 cut(s) 3609
Eco52I CGGCCG 1 cut(s) 1093
Eco57I CTGAAG 9 cut(s) 393, 417, 1311, 1704, 2600, 3125, 3621, 3939, 4049
Eco72I CACGTG 1 cut(s) 1523
Eco81I CCTNAGG 1 cut(s) 3702
Eco88I CYCGRG 2 cut(s) 1826, 3057
EcoO109I RGGNCCY 2 cut(s) 3998, 5074
EcoRI GAATTC 2 cut(s) 84, 2589
EcoRII CCWGG 5 cut(s) 63, 1499, 3022, 4071, 4893
EcoRV GATATC 1 cut(s) 145
EcoT14I CCWWGG 4 cut(s) 2761, 3070, 4210, 4369
EcoT22I ATGCAT 1 cut(s) 3946
EcoT38I GRGCYC 1 cut(s) 4002
ErhI CCWWGG 4 cut(s) 2761, 3070, 4210, 4369
Esp3I CGTCTC 1 cut(s) 3810
FalI AAGNNNNNCTT 2 cut(s) 3594, 3626
FaqI GGGAC 7 cut(s) 483, 1198, 1387, 2789, 3340, 4007, 4926
FauI CCCGC 2 cut(s) 329, 1088
FauNDI CATATG 4 cut(s) 1162, 1371, 2197, 2514
FblI GTMKAC 1 cut(s) 1816
Fnu4HI GCNGC 8 cut(s) 708, 1758, 2395, 2963, 3906, 3909, 3958, 4365
FriOI GRGCYC 1 cut(s) 4002
FseI GGCCGGCC 1 cut(s) 2167
Fsp4HI GCNGC 8 cut(s) 708, 1758, 2395, 2963, 3906, 3909, 3958, 4365
FspBI CTAG 7 cut(s) 192, 597, 651, 966, 1655, 4485, 4683
GlaI GCGC 5 cut(s) 1796, 3021, 3159, 3609, 4814
GluI GCNGC 8 cut(s) 708, 1758, 2395, 2963, 3906, 3909, 3958, 4365
GsuI CTGGAG 3 cut(s) 1775, 3254, 4260
HaeII RGCGCY 1 cut(s) 3611
HapII CCGG 6 cut(s) 1646, 1838, 2164, 3063, 4851, 5107
HgaI GACGC 3 cut(s) 3827, 4190, 5200
HhaI GCGC 5 cut(s) 1797, 3022, 3160, 3610, 4815
Hin6I GCGC 5 cut(s) 1795, 3020, 3158, 3608, 4813
HinP1I GCGC 5 cut(s) 1795, 3020, 3158, 3608, 4813
HincII GTYRAC 3 cut(s) 2129, 3187, 5112
HindII GTYRAC 3 cut(s) 2129, 3187, 5112
HindIII AAGCTT 3 cut(s) 1859, 2849, 4539
HpaI GTTAAC 2 cut(s) 3187, 5112
HpaII CCGG 6 cut(s) 1646, 1838, 2164, 3063, 4851, 5107
Hpy166II GTNNAC 9 cut(s) 179, 1073, 1817, 2129, 2301, 3187, 4061, 5112, 5207
Hpy8I GTNNAC 9 cut(s) 179, 1073, 1817, 2129, 2301, 3187, 4061, 5112, 5207
HpyCH4IV ACGT 4 cut(s) 1522, 4237, 4660, 5233
HpySE526I ACGT 4 cut(s) 1522, 4237, 4660, 5233
HspAI GCGC 5 cut(s) 1795, 3020, 3158, 3608, 4813
Kpn2I TCCGGA 1 cut(s) 1645
KpnI GGTACC 1 cut(s) 5106
KroI GCCGGC 1 cut(s) 2163
KroNI GCCGGC 1 cut(s) 2165
KspAI GTTAAC 2 cut(s) 3187, 5112
LmnI GCTCC 7 cut(s) 1803, 2076, 3619, 3757, 3767, 4279, 4321
Lsp1109I GCAGC 7 cut(s) 694, 1744, 2381, 3917, 3920, 3944, 4376
MaeI CTAG 7 cut(s) 192, 597, 651, 966, 1655, 4485, 4683
MaeII ACGT 4 cut(s) 1522, 4237, 4660, 5233
MaeIII GTNAC 7 cut(s) 626, 1037, 1088, 1244, 1337, 3202, 3742
MflI RGATCY 5 cut(s) 392, 697, 1321, 1567, 1786
MhlI GDGCHC 3 cut(s) 1266, 4002, 4318
MmeI TCCRAC 8 cut(s) 351, 370, 1880, 2895, 3282, 3551, 4145, 4548
Mph1103I ATGCAT 1 cut(s) 3946
MroI TCCGGA 1 cut(s) 1645
MroNI GCCGGC 1 cut(s) 2163
MroXI GAANNNNTTC 3 cut(s) 84, 2589, 3228
MslI CAYNNNNRTG 7 cut(s) 155, 186, 381, 921, 2766, 3015, 4896
MspA1I CMGCKG 3 cut(s) 1016, 2962, 3092
MspI CCGG 6 cut(s) 1646, 1838, 2164, 3063, 4851, 5107
MspR9I CCNGG 7 cut(s) 65, 1501, 3024, 4073, 4852, 4895, 5107
Mva1269I GAATGC 3 cut(s) 481, 1993, 4387
MvaI CCWGG 5 cut(s) 65, 1501, 3024, 4073, 4895
MvnI CGCG 1 cut(s) 5194
NaeI GCCGGC 1 cut(s) 2165
NciI CCSGG 2 cut(s) 4852, 5107
NcoI CCATGG 2 cut(s) 2761, 4210
NdeI CATATG 4 cut(s) 1162, 1371, 2197, 2514
NgoMIV GCCGGC 1 cut(s) 2163
NmeAIII GCCGAG 1 cut(s) 2141
NmuCI GTSAC 4 cut(s) 1037, 1244, 1337, 3202
NsiI ATGCAT 1 cut(s) 3946
NspI RCATGY 1 cut(s) 1893
NspV TTCGAA 2 cut(s) 2539, 4723
PaeR7I CTCGAG 2 cut(s) 1826, 3057
PagI TCATGA 2 cut(s) 1540, 5002
PasI CCCWGGG 1 cut(s) 64
PceI AGGCCT 1 cut(s) 2527
PcsI WCGNNNNNNNCGW 2 cut(s) 2113, 4087
PctI GAATGC 3 cut(s) 481, 1993, 4387
PdiI GCCGGC 1 cut(s) 2165
PdmI GAANNNNTTC 3 cut(s) 84, 2589, 3228
PflMI CCANNNNNTGG 3 cut(s) 3004, 3761, 4900
PkrI GCNGC 8 cut(s) 709, 1759, 2396, 2964, 3907, 3910, 3959, 4366
PmaCI CACGTG 1 cut(s) 1523
PmlI CACGTG 1 cut(s) 1523
Ppu21I YACGTR 2 cut(s) 1523, 5234
PsiI TTATAA 1 cut(s) 2342
Psp6I CCWGG 5 cut(s) 63, 1499, 3022, 4071, 4893
PspCI CACGTG 1 cut(s) 1523
PspGI CCWGG 5 cut(s) 63, 1499, 3022, 4071, 4893
PspOMI GGGCCC 1 cut(s) 3998
PspPI GGNCC 9 cut(s) 373, 1643, 2006, 2049, 3998, 3999, 4910, 4940, 5074
PspXI VCTCGAGB 2 cut(s) 1826, 3057
PsrI GAACNNNNNNTAC 2 cut(s) 4577, 4609
PstI CTGCAG 2 cut(s) 499, 3490
PstNI CAGNNNCTG 1 cut(s) 4072
PsuI RGATCY 5 cut(s) 392, 697, 1321, 1567, 1786
RigI GGCCGGCC 1 cut(s) 2167
RseI CAYNNNNRTG 7 cut(s) 155, 186, 381, 921, 2766, 3015, 4896
SatI GCNGC 8 cut(s) 708, 1758, 2395, 2963, 3906, 3909, 3958, 4365
Sau96I GGNCC 9 cut(s) 373, 1643, 2006, 2049, 3998, 3999, 4910, 4940, 5074
ScaI AGTACT 1 cut(s) 2627
ScrFI CCNGG 7 cut(s) 65, 1501, 3024, 4073, 4852, 4895, 5107
SduI GDGCHC 3 cut(s) 1266, 4002, 4318
SfcI CTRYAG 5 cut(s) 96, 495, 562, 3193, 3486
Sfr274I CTCGAG 2 cut(s) 1826, 3057
SfuI TTCGAA 2 cut(s) 2539, 4723
SinI GGWCC 3 cut(s) 373, 1643, 4940
SlaI CTCGAG 2 cut(s) 1826, 3057
SmiMI CAYNNNNRTG 7 cut(s) 155, 186, 381, 921, 2766, 3015, 4896
SmlI CTYRAG 6 cut(s) 1826, 1877, 2779, 3057, 3427, 3777
SmoI CTYRAG 6 cut(s) 1826, 1877, 2779, 3057, 3427, 3777
SpeI ACTAGT 1 cut(s) 4682
SseBI AGGCCT 1 cut(s) 2527
SspI AATATT 4 cut(s) 521, 2086, 2704, 3108
SspMI CTAG 7 cut(s) 192, 597, 651, 966, 1655, 4485, 4683
StuI AGGCCT 1 cut(s) 2527
StyD4I CCNGG 7 cut(s) 63, 1499, 3022, 4071, 4850, 4893, 5105
StyI CCWWGG 4 cut(s) 2761, 3070, 4210, 4369
TaiI ACGT 4 cut(s) 1525, 4240, 4663, 5236
TatI WGTACW 5 cut(s) 1072, 1768, 2298, 2625, 2975
TauI GCSGC 1 cut(s) 2965
TseFI GTSAC 4 cut(s) 1037, 1244, 1337, 3202
TseI GCWGC 7 cut(s) 707, 1757, 2394, 3905, 3908, 3957, 4364
Tsp45I GTSAC 4 cut(s) 1037, 1244, 1337, 3202
TspGWI ACGGA 4 cut(s) 571, 1334, 1834, 5047
Van91I CCANNNNNTGG 3 cut(s) 3004, 3761, 4900
VpaK11BI GGWCC 3 cut(s) 373, 1643, 4940
XbaI TCTAGA 2 cut(s) 650, 4484
XceI RCATGY 1 cut(s) 1893
XcmI CCANNNNNNNNNTGG 1 cut(s) 2768
XhoI CTCGAG 2 cut(s) 1826, 3057
XmiI GTMKAC 1 cut(s) 1816
XmnI GAANNNNTTC 3 cut(s) 84, 2589, 3228
XspI CTAG 7 cut(s) 192, 597, 651, 966, 1655, 4485, 4683
ZrmI AGTACT 1 cut(s) 2627
Zsp2I ATGCAT 1 cut(s) 3946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.