MD03G1168800.v1.1

chromodomain-helicase-DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007957
Physical Location & Seq
Reverse (-)
22814823 .. 22835513
20691 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1168800.v1.1.491

Sequence Viewer

Length: 5334 bp
ATGGCTTTTTTTAGGAACTATTCTAACGACACAGTTTCACGAAGTGTCCTGGAAGAAAAAACCCTAGGACAAAGTGTTGAAAGAATTCATAGCTCCACAGGGAATGAAGATGTGGATGTAAATTCTTATGAAAAGGAATTTGATATCAACATGGATGTTCAGTATCAAAGTGAAGGCGAACATGAAGATGCTAGTAGGTTGCACAATGAGGCAGCAGAAGATGAGGTTATTGGTACAAAATTTTCCAATTTACCCACTTCTGGGAGGAGAACAGCTGTGGCTGGAAAGTGGGGTTCAACCTTTTGGAAGGACTGTCAACCTATGCGCTCACAGGGTGGGTCTGATTCTGGTCAGGAAACTAAAAGTGGGTTGGACTACAGAAATGCTGTTGGATCTGAAGATAATTCTTCGGATGTCAAGGAAGATGGAATAGATTTTGAAGATGAGGGCAAGCCGAAAGTAAGCAAGGGACAACGGGGCCATTCTGACATCCCTGCAGATGAAATGTTATCTGATGAATATTATGAGCAGGATGGGGAAGAACAGAGTGAATCAATGCGCTATAGAGGGTTTCATCACTCCATTGGGTCAAATTCTAGGCCACAATCAAAGCCTAATTCAGTCAGCGACCGTGCACACTATAGAACTTATAGAAATGATATTGAAGATGGTGATGATGCCAACGAAGATCTTGATGCTGCTGATGCTGATTATGAAGAAGACGAAGATGATGATCCTGATGATGCCGATTTTGAACCTGATTCTGGTGTTGGAAGTGGTCGCACTGCACAGAAGGATAAAAATTGGAATGGTGAAGATTCTGATGATGATAATGATAGTGATGATGAGCTTGATGTTTCAGATGAAGATGATTCTTATTATGGAAAGAAACCTAAGAGTAGGCATCGTGGTAATGCTAAATCTACAAGGGAACGCAAATCATATCATTCATCTAGTCGGCAAAGAAGAGGGAGGTCATCGTTTGATGATGATGAGTCCTCAGCAGAGGACTCCGAAAGTGACAGTGACGAGGATTTTAAAAGTATTAAAAGGAAAGGTGTACATACGCGCAAGAAGAATGGCCGAAGAAATGTTACTGGACAAAATGGTGAGGTACGAACTTCTACTAGGTCTGTTAGGAAGGTCTCATATGTTGAAAGTGATGGAAGTGATGAAGTTGAAGAAGGAAAGAAAAAGAAGTCCCAGAAGGAGGAGATTGAAGAAGAGGATGGTGACTATATTGAAAAGGTGCTTTGGCATCAGCCTAAGGGCATGGCTGAAGATGCTCTGAGGAACAATAGATCAACGGATCCTGTGCTATTAAGTCACTTGTTTGATTCTGAGCCAGATTGGAATCATATGGAGTTCTTGATAAAATGGAAAGGGCAGTCCCATTTGCACTGTCAATGGAAATCAATTTCCGAGCTACAAAATCTTAGCGGTTTTAAGAAGGTTTTAAATTACACTAAAAAGGTGATGGAAGATGCCAGGTACAGGAACACAATATCACGTGAGGAGATTGAGGTTCATGATGTGAGCAAGGAAATGGACTTAGATCTCATCAAGCAGAATAGTCAGGTGGAGAGAATAATTGCAGACCGCATCAGGCAAGATACCTCTGGCGATGTGGGTCCGGAATATCTAGTCAAGTGGCAAGGATTGTCTTATGCTGAAGCAACATGGGAGAAGGATGTAGATATTGCGTTTGCACAAGATGCTATTGATGAGTTCAAGGTTCGTGAAGCTGCAATGGCTGTACAAGGGAAAATGGTGGATCTCCAGCGCAAGAGGAGCAAAGGAAGGGGGAAAAAGCTAGAGCTTTGTGGGGCTGTTTGGCGTTGGCAGTTTGAAACATTTGGTTTGTGTAGTCCGACATTCACCTCTCCCAGACCCTGCGTAAAGCGGGAGCCTTGTGCACTGGGAAGTCTAAGGAAGCTCGAGGAACAACCCGAATGGTTGAAGGGAGGAAAGCTTCGTGATTATCAGCTTGAGGCAGCTCTTGCATGTGGCAGTTGGAGGAATGATACCAATGTCATTTTAGCTGATGAAATGGGTCTTGGTAAAACAGTTCAATCAGTTTCAATGCTTGGATTTTTACAGAACGCTCAACAGATCCACGGGCCATTTCTTGTTGTTGTACCATTATCCACGTTGTCTAACTGGGCCAAAGAATTTAGGAAGTGGCTCCCTGATATGAATATTATTGTGTATGTTGGTACTCGTGCAAGCCGAGAGGTTTGTCAGCAGTATGAATTTAATAATGATAAAATAGTTGGCCGGCCTGTTAAATTCAATGCACTATTGACTACGTATGAGGTGGTTTTAAAGGATAAGGCTGTTCTTTCCAAGATCAAGTGGAATTATCTAATGGTTGATGAAGCTCATAGATTAAAGAACAGTGAGGCACAGTTGTACACAACCCTCTTGGAATTTAGCACAAAGAACAAGTTGCTTATAACTGGTACTCCCTTGCAGAACAGCGTAGAGGAGTTATGGGCTCTGCTTCACTTCCTCGACCCTGATAAATTCAAGAATAAAGATGATTTTGTTCAGAATTACAAAAATCTCAGTTCATTCAATGAGATTGAGCTTGCTAATCTGCATATGGAATTGAGGCCTCACATTCTTCGAAGAGTTATCAAGGATGTGGAAAAGTCTTTACCTCCTAAAATTGAGCGGATTCTTAGGGTTGAAATGTCTCCTCTTCAGAAACAGTATTATAAGTGGATTTTGGAACGCAACTTTCATAACTTGAACAAAGGAGTTCGTGGGAATCAGGTTTCGTTGTTGAATATTGTGGTAGAATTGAAGAAATGTTGTAATCATCCCTTTCTGTTTGAAAGTGCTGACCATGGTTATGGTGGGGACTCAAGTGGCAAAGATGGCAGTAAATTGGAGAGGACTATTTTAAGCAGCGGAAAGTTGGTTATACTTGATAAGCTTCTTATGAAATTGCATGAAACAAAGCACCGTGTTCTCATATTTTCCCAGATGGTAAGAATGTTGGACATACTGGCGGAGTACATGTCAATTAGAGGTTTTCAATTTCAGCGGCTTGATGGAAGTACAAAGGCCGATTTACGCCACCAAGCAATGGAACATTTTAATGCGCCTGGTAGTGAAGACTTTTGCTTCCTCCTTTCAACTCGAGCAGGTGGCCTTGGTATCAACCTTGCCACAGCGGATACGGTTATAATATTCGACTCAGATTGGAATCCCCAAAACGACCTGCAGGCAATGAGCAGAGCTCACAGAATAGGGCAACAAGAAGTTGTTAACATCTATAGATTTGTCACAAGCAAAAGTGTCGAGGAAGATATCCTGGAGAGAGCTAAGAAAAAGATGGTGCTTGACCATTTGGTGATTCAAAAATTAAATGCAGAGGGTAGGTTGGAAAAGAAAGAATCTAAGAAAGGGACTTTATTTGACAAAAATGAGCTGTCTTCTATTCTACGATTCGGGGCTGAGGAACTTTTCAAGGAAGAAAAAAATGATGAAGAAAGCAAGAAAAGACTCTTAAGTATGGATATTGATGAGATTCTTGAAAGAGCAGAAAAGGTTGAAGAGAAGGAAACTGCAGAGGAAGGGAATGAATTGTTGAGTGCTTTTAAGGTTGCAAATTTTGGTACTGCTGAAGATGATGGTAGTTTTTGGAGTCGTTGGATAAAGCCTGAAGCAGTATCCCAAGCCGAAGAAGCTCTTGCTCCTCGTGCCACTAGGAATACTAAGAGCTATGCAGAAGCTGATCAGCCTGATAGAAGTAATAAAAGAAAAAAGAAAGAATCTGAGCCTCAGGAAAGAGTCCAGAAAAGACGGAAAGCAGATTATACAGTCACCTCAGTGCCCATGATTGACGGAGCATCTGCTCAAGTTAGGGGATGGTCTTCTGGGAACTTATCCAAGAGAGACGCTTTGCGATTTTCTCGTGCTGTTATGAAATTTGGGAATGAGAGCCAAATTGCCTTGATAGTTGAGGAGGTCGGTGGTGCAGTTGCAGCAGCTTCAACTGATGCACAGGTTGAGCTTTTCAATGCATTGATTGATGGCTGCACAGAAGCAGTGGAAGTGGGGAGTCTGGACCCGAAGGGGCCATTGCTGGATTTTTATGGTGTCCCTGTCAAAGCTGGTGATCTGCTCAACCGTGTTCGTGAACTTCAACACCTGGCAAAACGCATTAGTCGATATGACGATCCCGTTGCTCAGTTTCGTGTCTTGAAGTACCTCAAACCTTCAAATTGGTCGAAAGGTTGTGGCTGGAATCAAATTGATGATGCAAGATTGCTTATTGGAGTATACTACCATGGCTTTGGAAATTGGGAAAAGATACGTTTAGATGCAAGGCTTGGCCTTACAAAGAAAATTGCTCCAGTGGAACTTCAACACCATGAAACATTTTTACCCCGTGCTCCAAATTTGAGAGATCGGGCTAACGCTCTTTTGGAAATGGAAATTGCAGCTCTTAGTGGAAAGAATGCAAATGTTAAAGTGGGTCGTAAGGCTTCAAAGGAGAGGGAAGCTACTCGCAATGTTTCTTTAGCTTGCAGTGGGCCAAAGAAAGGAAAAACCAGTTCTTCTAGAGCTAATTTTCCAACAAACAAGGACAGACCACTGAAACCCCAGAAAGTTGAACCTTTAGTGAAAGAGGAGGGTGAAATGTCAGACGATGATGAAGTGTATGAGAAGTTTAAGGAGGAGAAATGGATGGAATGGTGTGAAGAGATGATGAAGGATGAAACTAAAACCTTAAATCGTCTTAAACGGTTGCAGATGATTAGTGCCAATCTCCCAAAGGAAAAGGTGCTTTCCCAGATTCGAAGTTATTTACAGCTTCTTGGACGAACGATAGATCAAATTGTTTTAGAAAATGAGAAGGAACCTTATGGACAAGATAGGATGAGCATGCGCTTGTGGAACTATGTTTCTACCTTTTCAAATCTGTCCGGGGAGAGACTTCATCAAATCTATTCAAAACTTAAACAGCACCAGGATGTGGAGGCTGGCCCTTCCCACATGAATGGCTCTGCTTCTGGTCCCTTTGGCAGAGACAATGACTCTTCTCCCTTCTCCTTCCATTCGGACAGGCAAAGAGGTCCTGATACAGCGAAGTTTGAAGCATGGAAACGGAGGAGGAGAGGGGAAGCTGATAGCAATATCCAAGTTCAGGCCCAATCTCAAAGAAATATGAATAATGGTACCCGATTAACTGATCCAAGCTCATTAGGGATTCTTGGTGCAGGACCGTCAGAAAATAAACGCGCTGTGAATGAAAGGCCTTATAGGACACGGCAGACAGGTTTACCAACAAAACAAGGTTTCACATCAGGTATTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chromo DUF4208 Helicase_C SNF2_N

Protein Analysis

1778

Amino Acids

202.29

Weight (kDa)

5.9

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chromo PF00385 526 - 578 1.3e-14 Chromo (CHRromatin Organisation MOdifier) domain
ResIII PF04851 657 - 823 5.2e-10 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 672 - 944 1.2e-82 SNF2-related domain
DEAD PF00270 672 - 828 6.1e-08 DEAD/DEAH box helicase
HDA2-3 PF11496 895 - 1119 1.2e-10 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 974 - 1087 6.5e-26 Helicase conserved C-terminal domain
HTH_CHD1_Hrp3 PF23588 1399 - 1480 1.4e-21 ATP-dependent helicase CHD1-2/hrp3 HTH domain
CHD1-like_C PF13907 1563 - 1657 7.4e-15 Chromodomain-helicase-DNA-binding protein 1-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2456, 2721, 3194
AarI CACCTGC 1 cut(s) 3143
Acc36I ACCTGC 2 cut(s) 3143, 3237
Acc65I GGTACC 1 cut(s) 5192
AccB1I GGYRCC 1 cut(s) 5192
AccB7I CCANNNNNTGG 2 cut(s) 3094, 4990
AccBSI CCGCTC 1 cut(s) 2677
AccI GTMKAC 1 cut(s) 4293
AccII CGCG 2 cut(s) 1069, 5256
AccIII TCCGGA 1 cut(s) 1633
AciI CCGC 8 cut(s) 1440, 1600, 1903, 2677, 2916, 3017, 3052, 3182
AclWI GGATC 8 cut(s) 400, 728, 1304, 1317, 1782, 2107, 4184, 5201
AcoI YGGCCR 2 cut(s) 1081, 2275
AcuI CTGAAG 6 cut(s) 417, 1299, 1692, 2690, 3654, 3693
AcvI CACGTG 1 cut(s) 1511
AdeI CACNNNGTG 2 cut(s) 44, 335
AfiI CCNNNNNNNGG 9 cut(s) 260, 261, 764, 1210, 1494, 3094, 4556, 4990, 5161
AflII CTTAAG 1 cut(s) 3517
AflIII ACRYGT 1 cut(s) 3024
AjnI CCWGG 6 cut(s) 48, 1487, 3112, 3321, 4161, 4983
AjuI GAANNNNNNNTTGG 2 cut(s) 2040, 2072
AleI CACNNNNGTG 1 cut(s) 3839
Alw21I GWGCWC 4 cut(s) 637, 1918, 3250, 4408
Alw26I GTCTC 5 cut(s) 1150, 2703, 3900, 4942, 5037
Alw44I GTGCAC 2 cut(s) 633, 1914
AlwI GGATC 8 cut(s) 400, 728, 1304, 1317, 1782, 2107, 4184, 5201
AlwNI CAGNNNCTG 2 cut(s) 1893, 3743
Ama87I CYCGRG 2 cut(s) 1937, 3147
Aor13HI TCCGGA 1 cut(s) 1633
ApaLI GTGCAC 2 cut(s) 633, 1914
ApeKI GCWGC 9 cut(s) 212, 698, 1745, 1994, 2913, 3995, 3998, 4047, 4454
Asp700I GAANNNNTTC 1 cut(s) 84
Asp718I GGTACC 1 cut(s) 5192
AspA2I CCTAGG 1 cut(s) 64
AspLEI GCGC 7 cut(s) 327, 561, 1071, 1785, 3112, 4905, 5258
AsuC2I CCSGG 1 cut(s) 4942
AsuII TTCGAA 2 cut(s) 2629, 4813
AvaI CYCGRG 2 cut(s) 1937, 3147
AvaII GGWCC 5 cut(s) 1631, 4078, 5030, 5090, 5237
AvrII CCTAGG 1 cut(s) 64
AxyI CCTNAGG 2 cut(s) 1266, 3792
BaeGI GKGCMC 3 cut(s) 637, 1918, 3846
BaeI ACNNNNGTAYC 2 cut(s) 2016, 2049
BamHI GGATCC 1 cut(s) 1309
BanI GGYRCC 1 cut(s) 5192
BanII GRGCYC 2 cut(s) 2500, 3250
BarI GAAGNNNNNNTAC 2 cut(s) 1078, 1110
BauI CACGAG 3 cut(s) 2220, 3708, 3924
BbrPI CACGTG 1 cut(s) 1511
BbsI GAAGAC 4 cut(s) 726, 3129, 3435, 3876
Bbv12I GWGCWC 4 cut(s) 637, 1918, 3250, 4408
BbvCI CCTCAGC 2 cut(s) 1000, 3465
BbvI GCAGC 9 cut(s) 224, 685, 1732, 2006, 2925, 4007, 4010, 4034, 4466
BceAI ACGGC 1 cut(s) 5300
BcgI CGANNNNNNTGC 4 cut(s) 3289, 3323, 4178, 4212
BciT130I CCWGG 6 cut(s) 50, 1489, 3114, 3323, 4163, 4985
BciVI GTATCC 2 cut(s) 3178, 3691
BclI TGATCA 1 cut(s) 3745
BcnI CCSGG 1 cut(s) 4942
BcoDI GTCTC 5 cut(s) 1150, 2703, 3900, 4942, 5037
BfaI CTAG 9 cut(s) 65, 192, 597, 954, 1128, 1643, 1814, 3717, 4575
BfmI CTRYAG 7 cut(s) 376, 495, 562, 640, 3230, 3283, 3576
BfrI CTTAAG 1 cut(s) 3517
BfuAI ACCTGC 2 cut(s) 3143, 3237
BfuI GTATCC 2 cut(s) 3178, 3691
BglII AGATCT 2 cut(s) 688, 1555
BlnI CCTAGG 1 cut(s) 64
Bme1390I CCNGG 7 cut(s) 50, 1489, 3114, 3323, 4163, 4942, 4985
Bme18I GGWCC 5 cut(s) 1631, 4078, 5030, 5090, 5237
BmeT110I CYCGRG 2 cut(s) 1937, 3147
BmrFI CCNGG 7 cut(s) 50, 1489, 3114, 3323, 4163, 4942, 4985
BmrI ACTGGG 2 cut(s) 1928, 2170
BmuI ACTGGG 2 cut(s) 1928, 2170
BpiI GAAGAC 4 cut(s) 726, 3129, 3435, 3876
BplI GAGNNNNNCTC 2 cut(s) 3232, 3264
BpmI CTGGAG 3 cut(s) 1763, 3344, 4350
Bpu10I CCTNAGC 2 cut(s) 1000, 3465
Bpu14I TTCGAA 2 cut(s) 2629, 4813
BpuEI CTTGAG 3 cut(s) 2009, 2854, 3852
BpuMI CCSGG 1 cut(s) 4942
BsaAI YACGTR 2 cut(s) 1511, 2310
BsaBI GATNNNNATC 3 cut(s) 732, 1353, 3213
BsaI GGTCTC 1 cut(s) 1150
BsaJI CCNNGG 6 cut(s) 64, 2116, 2851, 3160, 4300, 4941
BsaWI WCCGGW 1 cut(s) 1633
BsaXI ACNNNNNCTCC 4 cut(s) 2449, 2479, 3011, 3041
Bsc4I CCNNNNNNNGG 9 cut(s) 260, 261, 764, 1210, 1494, 3094, 4556, 4990, 5161
Bse118I RCCGGY 1 cut(s) 2277
Bse1I ACTGG 7 cut(s) 1102, 1923, 2165, 2464, 3018, 4367, 4566
Bse21I CCTNAGG 2 cut(s) 1266, 3792
Bse3DI GCAATG 5 cut(s) 1755, 3099, 3243, 4091, 4531
Bse8I GATNNNNATC 3 cut(s) 732, 1353, 3213
BseAI TCCGGA 1 cut(s) 1633
BseBI CCWGG 6 cut(s) 50, 1489, 3114, 3323, 4163, 4985
BseDI CCNNGG 6 cut(s) 64, 2116, 2851, 3160, 4300, 4941
BseJI GATNNNNATC 3 cut(s) 732, 1353, 3213
BseLI CCNNNNNNNGG 9 cut(s) 260, 261, 764, 1210, 1494, 3094, 4556, 4990, 5161
BseMI GCAATG 5 cut(s) 1755, 3099, 3243, 4091, 4531
BseNI ACTGG 7 cut(s) 1102, 1923, 2165, 2464, 3018, 4367, 4566
BseSI GKGCMC 3 cut(s) 637, 1918, 3846
BseXI GCAGC 9 cut(s) 224, 685, 1732, 2006, 2925, 4007, 4010, 4034, 4466
BsgI GTGCAG 4 cut(s) 771, 4008, 4033, 5253
Bsh1236I CGCG 2 cut(s) 1069, 5256
Bsh1285I CGRYCG 1 cut(s) 631
BshNI GGYRCC 1 cut(s) 5192
BsiEI CGRYCG 1 cut(s) 631
BsiHKAI GWGCWC 4 cut(s) 637, 1918, 3250, 4408
BsiHKCI CYCGRG 2 cut(s) 1937, 3147
BsiSI CCGG 3 cut(s) 1634, 2278, 4941
BslFI GGGAC 7 cut(s) 483, 1186, 1375, 2879, 3430, 4097, 5016
BslI CCNNNNNNNGG 9 cut(s) 260, 261, 764, 1210, 1494, 3094, 4556, 4990, 5161
BsmAI GTCTC 5 cut(s) 1150, 2703, 3900, 4942, 5037
BsmBI CGTCTC 1 cut(s) 3900
BsmFI GGGAC 7 cut(s) 483, 1186, 1375, 2879, 3430, 4097, 5016
BsmI GAATGC 1 cut(s) 4477
Bso31I GGTCTC 1 cut(s) 1150
BsoBI CYCGRG 2 cut(s) 1937, 3147
Bsp119I TTCGAA 2 cut(s) 2629, 4813
Bsp1286I GDGCHC 6 cut(s) 637, 1918, 2500, 3250, 3846, 4408
Bsp13I TCCGGA 1 cut(s) 1633
Bsp1407I TGTACA 3 cut(s) 1060, 1756, 2412
Bsp19I CCATGG 2 cut(s) 2851, 4300
BspACI CCGC 8 cut(s) 1440, 1600, 1903, 2677, 2916, 3017, 3052, 3182
BspEI TCCGGA 1 cut(s) 1633
BspFNI CGCG 2 cut(s) 1069, 5256
BspHI TCATGA 1 cut(s) 1528
BspMAI CTGCAG 3 cut(s) 499, 3234, 3580
BspMI ACCTGC 2 cut(s) 3143, 3237
BspPI GGATC 8 cut(s) 400, 728, 1304, 1317, 1782, 2107, 4184, 5201
BspT104I TTCGAA 2 cut(s) 2629, 4813
BspT107I GGYRCC 1 cut(s) 5192
BspTI CTTAAG 1 cut(s) 3517
BspTNI GGTCTC 1 cut(s) 1150
BsrBI CCGCTC 1 cut(s) 2677
BsrDI GCAATG 5 cut(s) 1755, 3099, 3243, 4091, 4531
BsrFI RCCGGY 1 cut(s) 2277
BsrGI TGTACA 3 cut(s) 1060, 1756, 2412
BsrI ACTGG 7 cut(s) 1102, 1923, 2165, 2464, 3018, 4367, 4566
BssAI RCCGGY 1 cut(s) 2277
BssECI CCNNGG 6 cut(s) 64, 2116, 2851, 3160, 4300, 4941
BssNAI GTATAC 1 cut(s) 4294
BssSI CACGAG 3 cut(s) 2220, 3708, 3924
BssT1I CCWWGG 4 cut(s) 64, 2851, 3160, 4300
Bst1107I GTATAC 1 cut(s) 4294
Bst2BI CACGAG 3 cut(s) 2220, 3708, 3924
Bst2UI CCWGG 6 cut(s) 50, 1489, 3114, 3323, 4163, 4985
Bst6I CTCTTC 7 cut(s) 961, 1218, 2626, 2709, 3558, 4710, 5059
BstAFI CTTAAG 1 cut(s) 3517
BstAPI GCANNNNNTGC 2 cut(s) 1715, 2000
BstAUI TGTACA 3 cut(s) 1060, 1756, 2412
BstBAI YACGTR 2 cut(s) 1511, 2310
BstBI TTCGAA 2 cut(s) 2629, 4813
BstC8I GCNNGC 8 cut(s) 452, 2227, 2279, 2592, 3234, 4540, 4901, 4999
BstDSI CCRYGG 3 cut(s) 2116, 2851, 4300
BstFNI CGCG 2 cut(s) 1069, 5256
BstHHI GCGC 7 cut(s) 327, 561, 1071, 1785, 3112, 4905, 5258
BstMAI GTCTC 5 cut(s) 1150, 2703, 3900, 4942, 5037
BstMCI CGRYCG 1 cut(s) 631
BstNI CCWGG 6 cut(s) 50, 1489, 3114, 3323, 4163, 4985
BstNSI RCATGY 3 cut(s) 2007, 3028, 4903
BstSCI CCNGG 7 cut(s) 48, 1487, 3112, 3321, 4161, 4940, 4983
BstSFI CTRYAG 7 cut(s) 376, 495, 562, 640, 3230, 3283, 3576
BstSLI GKGCMC 3 cut(s) 637, 1918, 3846
BstSNI TACGTA 1 cut(s) 2310
BstUI CGCG 2 cut(s) 1069, 5256
BstV1I GCAGC 9 cut(s) 224, 685, 1732, 2006, 2925, 4007, 4010, 4034, 4466
BstV2I GAAGAC 4 cut(s) 726, 3129, 3435, 3876
BstX2I RGATCY 6 cut(s) 392, 688, 1309, 1555, 1774, 2112
BstXI CCANNNNNNTGG 3 cut(s) 2858, 4307, 5015
BstYI RGATCY 6 cut(s) 392, 688, 1309, 1555, 1774, 2112
BstZ17I GTATAC 1 cut(s) 4294
Bsu36I CCTNAGG 2 cut(s) 1266, 3792
BsuI GTATCC 2 cut(s) 3178, 3691
BtgI CCRYGG 3 cut(s) 2116, 2851, 4300
BtgZI GCGATG 1 cut(s) 1638
BtsI GCAGTG 3 cut(s) 783, 4065, 4549
BveI ACCTGC 2 cut(s) 3143, 3237
Cac8I GCNNGC 8 cut(s) 452, 2227, 2279, 2592, 3234, 4540, 4901, 4999
CaiI CAGNNNCTG 2 cut(s) 1893, 3743
CciI TCATGA 1 cut(s) 1528
CfoI GCGC 7 cut(s) 327, 561, 1071, 1785, 3112, 4905, 5258
Cfr10I RCCGGY 1 cut(s) 2277
CseI GACGC 1 cut(s) 3917
DraI TTTAAA 3 cut(s) 1039, 1458, 2325
DraIII CACNNNGTG 2 cut(s) 44, 335
EaeI YGGCCR 2 cut(s) 1081, 2275
Eam1104I CTCTTC 7 cut(s) 961, 1218, 2626, 2709, 3558, 4710, 5059
EarI CTCTTC 7 cut(s) 961, 1218, 2626, 2709, 3558, 4710, 5059
EciI GGCGGA 1 cut(s) 3032
Ecl136II GAGCTC 1 cut(s) 3248
Eco105I TACGTA 1 cut(s) 2310
Eco130I CCWWGG 4 cut(s) 64, 2851, 3160, 4300
Eco147I AGGCCT 2 cut(s) 2617, 5272
Eco24I GRGCYC 2 cut(s) 2500, 3250
Eco31I GGTCTC 1 cut(s) 1150
Eco32I GATATC 2 cut(s) 145, 3319
Eco47I GGWCC 5 cut(s) 1631, 4078, 5030, 5090, 5237
Eco53kI GAGCTC 1 cut(s) 3248
Eco57I CTGAAG 6 cut(s) 417, 1299, 1692, 2690, 3654, 3693
Eco72I CACGTG 1 cut(s) 1511
Eco81I CCTNAGG 2 cut(s) 1266, 3792
Eco88I CYCGRG 2 cut(s) 1937, 3147
EcoICRI GAGCTC 1 cut(s) 3248
EcoO109I RGGNCCY 1 cut(s) 5090
EcoRI GAATTC 1 cut(s) 84
EcoRII CCWGG 6 cut(s) 48, 1487, 3112, 3321, 4161, 4983
EcoRV GATATC 2 cut(s) 145, 3319
EcoT14I CCWWGG 4 cut(s) 64, 2851, 3160, 4300
EcoT22I ATGCAT 1 cut(s) 4036
EcoT38I GRGCYC 2 cut(s) 2500, 3250
ErhI CCWWGG 4 cut(s) 64, 2851, 3160, 4300
Esp3I CGTCTC 1 cut(s) 3900
FalI AAGNNNNNCTT 2 cut(s) 3684, 3716
FaqI GGGAC 7 cut(s) 483, 1186, 1375, 2879, 3430, 4097, 5016
FauI CCCGC 1 cut(s) 1896
FauNDI CATATG 3 cut(s) 1150, 1359, 2604
FbaI TGATCA 1 cut(s) 3745
FblI GTMKAC 1 cut(s) 4293
FriOI GRGCYC 2 cut(s) 2500, 3250
FseI GGCCGGCC 1 cut(s) 2281
FspBI CTAG 9 cut(s) 65, 192, 597, 954, 1128, 1643, 1814, 3717, 4575
GlaI GCGC 7 cut(s) 326, 560, 1070, 1784, 3111, 4904, 5257
GsuI CTGGAG 3 cut(s) 1763, 3344, 4350
HapII CCGG 3 cut(s) 1634, 2278, 4941
HgaI GACGC 1 cut(s) 3917
HhaI GCGC 7 cut(s) 327, 561, 1071, 1785, 3112, 4905, 5258
Hin6I GCGC 7 cut(s) 325, 559, 1069, 1783, 3110, 4903, 5256
HinP1I GCGC 7 cut(s) 325, 559, 1069, 1783, 3110, 4903, 5256
HincII GTYRAC 2 cut(s) 317, 3277
HindII GTYRAC 2 cut(s) 317, 3277
HindIII AAGCTT 2 cut(s) 1970, 2939
HpaI GTTAAC 1 cut(s) 3277
HpaII CCGG 3 cut(s) 1634, 2278, 4941
Hpy166II GTNNAC 9 cut(s) 317, 635, 1061, 1916, 2415, 3277, 4151, 4294, 5297
Hpy8I GTNNAC 9 cut(s) 317, 635, 1061, 1916, 2415, 3277, 4151, 4294, 5297
HpyCH4IV ACGT 4 cut(s) 1510, 2150, 2309, 4327
HpySE526I ACGT 4 cut(s) 1510, 2150, 2309, 4327
HspAI GCGC 7 cut(s) 325, 559, 1069, 1783, 3110, 4903, 5256
Kpn2I TCCGGA 1 cut(s) 1633
KpnI GGTACC 1 cut(s) 5196
KroI GCCGGC 1 cut(s) 2277
KroNI GCCGGC 1 cut(s) 2279
Ksp22I TGATCA 1 cut(s) 3745
KspAI GTTAAC 1 cut(s) 3277
LmnI GCTCC 8 cut(s) 98, 1791, 1906, 2190, 3709, 3857, 4369, 4411
Lsp1109I GCAGC 9 cut(s) 224, 685, 1732, 2006, 2925, 4007, 4010, 4034, 4466
MaeI CTAG 9 cut(s) 65, 192, 597, 954, 1128, 1643, 1814, 3717, 4575
MaeII ACGT 4 cut(s) 1510, 2150, 2309, 4327
MaeIII GTNAC 7 cut(s) 1019, 1025, 1093, 1232, 1325, 3292, 3832
MbiI CCGCTC 1 cut(s) 2677
MflI RGATCY 6 cut(s) 392, 688, 1309, 1555, 1774, 2112
MhlI GDGCHC 6 cut(s) 637, 1918, 2500, 3250, 3846, 4408
MlyI GAGTC 9 cut(s) 1004, 1004, 2861, 3197, 3507, 3664, 3810, 4081, 5045
MmeI TCCRAC 9 cut(s) 351, 370, 751, 1895, 1994, 2985, 3372, 3641, 4613
Mph1103I ATGCAT 1 cut(s) 4036
MroI TCCGGA 1 cut(s) 1633
MroNI GCCGGC 1 cut(s) 2277
MroXI GAANNNNTTC 1 cut(s) 84
MslI CAYNNNNRTG 6 cut(s) 186, 2856, 3105, 3839, 4986, 5013
MspA1I CMGCKG 4 cut(s) 275, 2916, 3052, 3182
MspCI CTTAAG 1 cut(s) 3517
MspI CCGG 3 cut(s) 1634, 2278, 4941
MspR9I CCNGG 7 cut(s) 50, 1489, 3114, 3323, 4163, 4942, 4985
Mva1269I GAATGC 1 cut(s) 4477
MvaI CCWGG 6 cut(s) 50, 1489, 3114, 3323, 4163, 4985
MvnI CGCG 2 cut(s) 1069, 5256
NaeI GCCGGC 1 cut(s) 2279
NciI CCSGG 1 cut(s) 4942
NcoI CCATGG 2 cut(s) 2851, 4300
NdeI CATATG 3 cut(s) 1150, 1359, 2604
NgoMIV GCCGGC 1 cut(s) 2277
NmeAIII GCCGAG 1 cut(s) 2255
NmuCI GTSAC 6 cut(s) 1019, 1025, 1232, 1325, 3292, 3832
NsiI ATGCAT 1 cut(s) 4036
NspI RCATGY 3 cut(s) 2007, 3028, 4903
NspV TTCGAA 2 cut(s) 2629, 4813
OliI CACNNNNGTG 1 cut(s) 3839
PaeI GCATGC 1 cut(s) 4903
PaeR7I CTCGAG 2 cut(s) 1937, 3147
PagI TCATGA 1 cut(s) 1528
PaqCI CACCTGC 1 cut(s) 3143
PceI AGGCCT 2 cut(s) 2617, 5272
PciI ACATGT 1 cut(s) 3024
PcsI WCGNNNNNNNCGW 2 cut(s) 2227, 4177
PctI GAATGC 1 cut(s) 4477
PdiI GCCGGC 1 cut(s) 2279
PdmI GAANNNNTTC 1 cut(s) 84
PflMI CCANNNNNTGG 2 cut(s) 3094, 4990
PfoI TCCNGGA 2 cut(s) 48, 3321
PleI GAGTC 9 cut(s) 1003, 1004, 2861, 3197, 3507, 3663, 3809, 4080, 5045
PmaCI CACGTG 1 cut(s) 1511
PmlI CACGTG 1 cut(s) 1511
PpsI GAGTC 9 cut(s) 1003, 1004, 2861, 3197, 3507, 3663, 3809, 4080, 5045
Ppu21I YACGTR 2 cut(s) 1511, 2310
PpuMI RGGWCCY 1 cut(s) 5090
PscI ACATGT 1 cut(s) 3024
PsiI TTATAA 3 cut(s) 2456, 2721, 3194
Psp124BI GAGCTC 1 cut(s) 3250
Psp5II RGGWCCY 1 cut(s) 5090
Psp6I CCWGG 6 cut(s) 48, 1487, 3112, 3321, 4161, 4983
PspCI CACGTG 1 cut(s) 1511
PspGI CCWGG 6 cut(s) 48, 1487, 3112, 3321, 4161, 4983
PspPPI RGGWCCY 1 cut(s) 5090
PspXI VCTCGAGB 2 cut(s) 1937, 3147
PstI CTGCAG 3 cut(s) 499, 3234, 3580
PstNI CAGNNNCTG 2 cut(s) 1893, 3743
PsuI RGATCY 6 cut(s) 392, 688, 1309, 1555, 1774, 2112
PvuII CAGCTG 1 cut(s) 275
RigI GGCCGGCC 1 cut(s) 2281
RseI CAYNNNNRTG 6 cut(s) 186, 2856, 3105, 3839, 4986, 5013
SacI GAGCTC 1 cut(s) 3250
SbfI CCTGCAGG 1 cut(s) 3234
SchI GAGTC 9 cut(s) 1004, 1004, 2861, 3197, 3507, 3664, 3810, 4081, 5045
ScrFI CCNGG 7 cut(s) 50, 1489, 3114, 3323, 4163, 4942, 4985
SdaI CCTGCAGG 1 cut(s) 3234
SduI GDGCHC 6 cut(s) 637, 1918, 2500, 3250, 3846, 4408
SfcI CTRYAG 7 cut(s) 376, 495, 562, 640, 3230, 3283, 3576
Sfr274I CTCGAG 2 cut(s) 1937, 3147
SfuI TTCGAA 2 cut(s) 2629, 4813
SinI GGWCC 5 cut(s) 1631, 4078, 5030, 5090, 5237
SlaI CTCGAG 2 cut(s) 1937, 3147
SmiMI CAYNNNNRTG 6 cut(s) 186, 2856, 3105, 3839, 4986, 5013
SmlI CTYRAG 6 cut(s) 1937, 1988, 2869, 3147, 3517, 3867
SmoI CTYRAG 6 cut(s) 1937, 1988, 2869, 3147, 3517, 3867
SnaBI TACGTA 1 cut(s) 2310
SphI GCATGC 1 cut(s) 4903
Sse8387I CCTGCAGG 1 cut(s) 3234
SseBI AGGCCT 2 cut(s) 2617, 5272
SsiI CCGC 8 cut(s) 1440, 1600, 1903, 2677, 2916, 3017, 3052, 3182
SspI AATATT 4 cut(s) 521, 2200, 2794, 3198
SspMI CTAG 9 cut(s) 65, 192, 597, 954, 1128, 1643, 1814, 3717, 4575
SstI GAGCTC 1 cut(s) 3250
StuI AGGCCT 2 cut(s) 2617, 5272
StyD4I CCNGG 7 cut(s) 48, 1487, 3112, 3321, 4161, 4940, 4983
StyI CCWWGG 4 cut(s) 64, 2851, 3160, 4300
TaiI ACGT 4 cut(s) 1513, 2153, 2312, 4330
TaqI TCGA 9 cut(s) 1938, 2514, 2629, 3148, 3201, 3309, 4180, 4241, 4813
TatI WGTACW 5 cut(s) 1060, 1756, 2412, 3021, 3065
TauI GCSGC 1 cut(s) 3055
TseFI GTSAC 6 cut(s) 1019, 1025, 1232, 1325, 3292, 3832
TseI GCWGC 9 cut(s) 212, 698, 1745, 1994, 2913, 3995, 3998, 4047, 4454
Tsp45I GTSAC 6 cut(s) 1019, 1025, 1232, 1325, 3292, 3832
TspGWI ACGGA 4 cut(s) 1322, 3829, 3870, 5137
Van91I CCANNNNNTGG 2 cut(s) 3094, 4990
Vha464I CTTAAG 1 cut(s) 3517
VneI GTGCAC 2 cut(s) 633, 1914
VpaK11BI GGWCC 5 cut(s) 1631, 4078, 5030, 5090, 5237
XbaI TCTAGA 1 cut(s) 4574
XceI RCATGY 3 cut(s) 2007, 3028, 4903
XcmI CCANNNNNNNNNTGG 1 cut(s) 2858
XhoI CTCGAG 2 cut(s) 1937, 3147
XmaJI CCTAGG 1 cut(s) 64
XmiI GTMKAC 1 cut(s) 4293
XmnI GAANNNNTTC 1 cut(s) 84
XspI CTAG 9 cut(s) 65, 192, 597, 954, 1128, 1643, 1814, 3717, 4575
Zsp2I ATGCAT 1 cut(s) 4036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.