FvH4_3g28570
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014699
Physical Location & Seq
Forward (+)
21587138 .. 21589879
2742 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g28570.t1

Sequence Viewer

Length: 1581 bp
ATGTCTTCTCCCTCAAAGAGTCCAACTATGGAGCCTCCTGTTCAACAAAGTCATCCGCATCCCAAGGCTTCGAATTCTGTTACCGAAGCTACTGCTGACAGTGGTAGCCCTAGTCCTAAGCCACTGCAAAAAAATCCACATGAAGCAGCAGCTCGTACTCATCATCATGTTGCCCATAATAATGGTGCCTCATCGGTTGATCATCATCGCTTCTCCGATGAGGAAGAGGAGCTGAACAACTTTAACAACAATCAGAAGAAGCAGCAGGATGCTTTTCTTAGCAGCTTTCCAGCTGGATATAGGTTCAACCCTCGTGATGAAGAACTTGTGCGGTATTACCTGCAGAAAAAGATCATGGACGAGCCATTGCCTCCCAACAAGATTATGGAAGTTAATCTCTACCGCTACAATCCTGAGACGCTTGCTGGCAAATATGATACTTATGGAGAAAAAGAATGGTACTTCTTTACTCCAAGAGACCGTAAGTATCGCAATGGTCAACGACCAAATAGGGCAGCTGGCGATGGTTATTGGAAGGCTACAGGAGCAGACAAATCAATAAAGTCTGGTAATATAGAGGTTGGTTTTAGAAAAGCTCTCGTTTTCTACAGAGGCAAACCTCCAAAGGGAAAAAAGACCAATTGGATCATGCATGAGTACCGAGTCCCTGATTCACGTACTGCCAAAAGGACTGGAAATGACATGAGGTTGGATAACTGGGTGCTCTGTAGAATTTATAAGAAAGCTAATAACAAGAACAAAATCCAGACTCGAAATCATGCAGTAGATCCTGGTGAAGAACATATCTCTATGTCAATAACAGATGACACGGAGATGGAGATGAACGGTCAAGTTGATGACACTCAAACAACGGAGTCTGAAGAAGAATACACACACACCATTGCTCATCCACCATTGGAGTTTGACGGGGCATATCCTACTTTTGCATATCATTATACATCTTACCCGGGGGTTGCTGCACTTGACAATAACATGCTTCAACCGGTCGTTATGAGTTATAAGGATGGTTATGCAAACATATTTCCTTCGCTACAGACTAGTGGATCTTCTTCAGCACTGCAATGTCTTGATCAAAACGGTAAAGTGCCAATCTCCCATCATAAGCTGCATCCAGTTGATATCACATCAAAATCCATAGTATGTAAAGAAGAAGCATCAGATCAAATGTATGGCGTGCAATTTCCAAACCAAAAGTTTATGCAATCTATTGAGGGCATCACATCAAATTCAACGGTATGCACCGACGAGGCATGTTCTGATCAACAATATGGTATGCAATTTCCGAGTCAAGAATATATTGTGCATTCACTTGATGGTAGAGCACAAACAAAAACACATTCTTGTGGAAAGAGCGTTCGACCAGGTGATGCAGTTGATTTTGGACCTCCATCAGTGCCTTATCTTGATCAAACGAGCATTCAACAAAAAATTGGTAAGCCTTTGTTATTACCATCATCTGATGGTCTGCAATATATCATAGAAGAATCAAATGATGAACATGATAATCTCTTCTTTGATTGTCCACACTTTACGTATTCAGAACCTTTTCCGTGGCCATGA

Protein Analysis

527

Amino Acids

59.56

Weight (kDa)

6.0

Isoelectric Point (pI)

46.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 97 - 222 5.5e-45 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 738, 1020
Acc36I ACCTGC 1 cut(s) 348
AccB1I GGYRCC 1 cut(s) 185
AciI CCGC 3 cut(s) 56, 331, 403
AclWI GGATC 3 cut(s) 653, 782, 1072
AcoI YGGCCR 1 cut(s) 1574
AcsI RAATTY 3 cut(s) 73, 732, 1246
AcuI CTGAAG 2 cut(s) 900, 1056
AfaI GTAC 4 cut(s) 157, 461, 659, 679
AfiI CCNNNNNNNGG 1 cut(s) 626
AgeI ACCGGT 1 cut(s) 1003
AgsI TTSAA 5 cut(s) 44, 307, 1001, 1251, 1442
AhlI ACTAGT 1 cut(s) 1058
AjnI CCWGG 2 cut(s) 790, 1381
AloI GAACNNNNNNTCC 2 cut(s) 1359, 1391
AluBI AGCT 9 cut(s) 89, 152, 232, 285, 293, 518, 596, 746, 1126
AluI AGCT 9 cut(s) 89, 152, 232, 285, 293, 518, 596, 746, 1126
Alw21I GWGCWC 2 cut(s) 726, 1345
Alw26I GTCTC 2 cut(s) 410, 471
AlwI GGATC 3 cut(s) 653, 782, 1072
Ama87I CYCGRG 1 cut(s) 967
AoxI GGCC 1 cut(s) 1574
ApeKI GCWGC 7 cut(s) 146, 149, 262, 282, 515, 977, 1126
ApoI RAATTY 3 cut(s) 73, 732, 1246
AsiGI ACCGGT 1 cut(s) 1003
Asp700I GAANNNNTTC 1 cut(s) 1566
AspS9I GGNCC 1 cut(s) 1403
AsuC2I CCSGG 2 cut(s) 968, 969
AsuHPI GGTGA 2 cut(s) 806, 1397
AsuII TTCGAA 1 cut(s) 71
AvaI CYCGRG 1 cut(s) 967
AvaII GGWCC 1 cut(s) 1403
BalI TGGCCA 1 cut(s) 1576
BanI GGYRCC 1 cut(s) 185
BauI CACGAG 1 cut(s) 312
Bbv12I GWGCWC 2 cut(s) 726, 1345
BbvI GCAGC 7 cut(s) 158, 161, 274, 294, 527, 964, 1113
BccI CCATC 8 cut(s) 518, 829, 1019, 1125, 1328, 1417, 1475, 1480
BcgI CGANNNNNNTGC 2 cut(s) 74, 108
BciT130I CCWGG 2 cut(s) 792, 1383
BclI TGATCA 4 cut(s) 199, 1090, 1279, 1426
BcnI CCSGG 2 cut(s) 968, 969
BcoDI GTCTC 2 cut(s) 410, 471
BcuI ACTAGT 1 cut(s) 1058
BfaI CTAG 2 cut(s) 111, 1059
BfmI CTRYAG 5 cut(s) 341, 540, 607, 727, 1052
BfuAI ACCTGC 1 cut(s) 348
BisI GCNGC 7 cut(s) 147, 150, 263, 283, 516, 978, 1127
BlsI GCNGC 7 cut(s) 148, 151, 264, 284, 517, 979, 1128
Bme1390I CCNGG 4 cut(s) 792, 968, 969, 1383
Bme18I GGWCC 1 cut(s) 1403
BmeT110I CYCGRG 1 cut(s) 967
BmgT120I GGNCC 1 cut(s) 1403
BmiI GGNNCC 2 cut(s) 33, 187
BmrFI CCNGG 4 cut(s) 792, 968, 969, 1383
BmrI ACTGGG 1 cut(s) 727
BmsI GCATC 6 cut(s) 67, 259, 1138, 1184, 1245, 1378
BmuI ACTGGG 1 cut(s) 727
Bpu10I CCTNAGC 1 cut(s) 117
Bpu14I TTCGAA 1 cut(s) 71
BpuMI CCSGG 2 cut(s) 968, 969
BsaAI YACGTR 2 cut(s) 677, 1554
BsaBI GATNNNNATC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 471
BsaJI CCNNGG 4 cut(s) 63, 967, 968, 1571
BsaWI WCCGGW 1 cut(s) 1003
Bsc4I CCNNNNNNNGG 1 cut(s) 626
Bse118I RCCGGY 1 cut(s) 1003
Bse1I ACTGG 3 cut(s) 697, 722, 1133
Bse3DI GCAATG 4 cut(s) 365, 499, 900, 1088
Bse8I GATNNNNATC 1 cut(s) 204
BseBI CCWGG 2 cut(s) 792, 1383
BseDI CCNNGG 4 cut(s) 63, 967, 968, 1571
BseGI GGATG 6 cut(s) 52, 58, 274, 907, 1030, 1129
BseJI GATNNNNATC 1 cut(s) 204
BseLI CCNNNNNNNGG 1 cut(s) 626
BseMI GCAATG 4 cut(s) 365, 499, 900, 1088
BseMII CTCAG 1 cut(s) 405
BseNI ACTGG 3 cut(s) 697, 722, 1133
BseRI GAGGAG 1 cut(s) 242
BseXI GCAGC 7 cut(s) 158, 161, 274, 294, 527, 964, 1113
BsgI GTGCAG 1 cut(s) 963
Bsh1285I CGRYCG 1 cut(s) 1008
BshFI GGCC 1 cut(s) 1576
BshNI GGYRCC 1 cut(s) 185
BshTI ACCGGT 1 cut(s) 1003
BsiEI CGRYCG 1 cut(s) 1008
BsiHKAI GWGCWC 2 cut(s) 726, 1345
BsiHKCI CYCGRG 1 cut(s) 967
BsiSI CCGG 2 cut(s) 968, 1004
BslFI GGGAC 1 cut(s) 650
BslI CCNNNNNNNGG 1 cut(s) 626
BsmAI GTCTC 2 cut(s) 410, 471
BsmBI CGTCTC 1 cut(s) 410
BsmFI GGGAC 1 cut(s) 650
BsmI GAATGC 2 cut(s) 1324, 1437
BsnI GGCC 1 cut(s) 1576
Bso31I GGTCTC 1 cut(s) 471
BsoBI CYCGRG 1 cut(s) 967
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 2 cut(s) 726, 1345
Bsp143I GATC 9 cut(s) 199, 351, 645, 787, 1064, 1090, 1180, 1279, 1426
BspACI CCGC 3 cut(s) 56, 331, 403
BspANI GGCC 1 cut(s) 1576
BspCNI CTCAG 1 cut(s) 406
BspLI GGNNCC 2 cut(s) 33, 187
BspMAI CTGCAG 1 cut(s) 345
BspMI ACCTGC 1 cut(s) 348
BspPI GGATC 3 cut(s) 653, 782, 1072
BspT104I TTCGAA 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 185
BspTNI GGTCTC 1 cut(s) 471
BsrDI GCAATG 4 cut(s) 365, 499, 900, 1088
BsrFI RCCGGY 1 cut(s) 1003
BsrI ACTGG 3 cut(s) 697, 722, 1133
BssAI RCCGGY 1 cut(s) 1003
BssECI CCNNGG 4 cut(s) 63, 967, 968, 1571
BssMI GATC 9 cut(s) 199, 351, 645, 787, 1064, 1090, 1180, 1279, 1426
BssSI CACGAG 1 cut(s) 312
BssT1I CCWWGG 1 cut(s) 63
Bst2BI CACGAG 1 cut(s) 312
Bst2UI CCWGG 2 cut(s) 792, 1383
Bst4CI ACNGT 5 cut(s) 101, 482, 848, 1100, 1255
Bst6I CTCTTC 2 cut(s) 219, 1535
BstBAI YACGTR 2 cut(s) 677, 1554
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 4 cut(s) 423, 427, 520, 1196
BstDEI CTNAG 3 cut(s) 117, 278, 414
BstDSI CCRYGG 1 cut(s) 1571
BstF5I GGATG 6 cut(s) 52, 58, 274, 907, 1030, 1129
BstKTI GATC 9 cut(s) 202, 354, 648, 790, 1067, 1093, 1183, 1282, 1429
BstMAI GTCTC 2 cut(s) 410, 471
BstMBI GATC 9 cut(s) 199, 351, 645, 787, 1064, 1090, 1180, 1279, 1426
BstMCI CGRYCG 1 cut(s) 1008
BstMWI GCNNNNNNNGC 1 cut(s) 545
BstNI CCWGG 2 cut(s) 792, 1383
BstNSI RCATGY 2 cut(s) 997, 1275
BstSCI CCNGG 4 cut(s) 790, 966, 967, 1381
BstSFI CTRYAG 5 cut(s) 341, 540, 607, 727, 1052
BstSNI TACGTA 1 cut(s) 1554
BstV1I GCAGC 7 cut(s) 158, 161, 274, 294, 527, 964, 1113
BstX2I RGATCY 2 cut(s) 787, 1064
BstXI CCANNNNNNTGG 1 cut(s) 182
BstYI RGATCY 2 cut(s) 787, 1064
BsuRI GGCC 1 cut(s) 1576
BtgI CCRYGG 1 cut(s) 1571
BtgZI GCGATG 2 cut(s) 191, 537
BtsCI GGATG 6 cut(s) 52, 58, 274, 907, 1030, 1129
BtsI GCAGTG 2 cut(s) 122, 1076
BtsIMutI CAGTG 4 cut(s) 106, 122, 1076, 1419
BveI ACCTGC 1 cut(s) 348
Cac8I GCNNGC 4 cut(s) 423, 427, 520, 1196
Cfr10I RCCGGY 1 cut(s) 1003
Cfr13I GGNCC 1 cut(s) 1403
Cfr9I CCCGGG 1 cut(s) 967
CseI GACGC 1 cut(s) 427
CsiI ACCWGGT 1 cut(s) 1381
Csp6I GTAC 4 cut(s) 156, 460, 658, 678
CspAI ACCGGT 1 cut(s) 1003
CviQI GTAC 4 cut(s) 156, 460, 658, 678
DdeI CTNAG 3 cut(s) 117, 278, 414
DpnI GATC 9 cut(s) 201, 353, 647, 789, 1066, 1092, 1182, 1281, 1428
DpnII GATC 9 cut(s) 199, 351, 645, 787, 1064, 1090, 1180, 1279, 1426
EaeI YGGCCR 1 cut(s) 1574
Eam1104I CTCTTC 2 cut(s) 219, 1535
EarI CTCTTC 2 cut(s) 219, 1535
Eco105I TACGTA 1 cut(s) 1554
Eco130I CCWWGG 1 cut(s) 63
Eco31I GGTCTC 1 cut(s) 471
Eco32I GATATC 1 cut(s) 1141
Eco47I GGWCC 1 cut(s) 1403
Eco57I CTGAAG 2 cut(s) 900, 1056
Eco88I CYCGRG 1 cut(s) 967
EcoRI GAATTC 1 cut(s) 73
EcoRII CCWGG 2 cut(s) 790, 1381
EcoRV GATATC 1 cut(s) 1141
EcoT14I CCWWGG 1 cut(s) 63
EcoT22I ATGCAT 1 cut(s) 654
ErhI CCWWGG 1 cut(s) 63
Esp3I CGTCTC 1 cut(s) 410
FaqI GGGAC 1 cut(s) 650
FbaI TGATCA 4 cut(s) 199, 1090, 1279, 1426
Fnu4HI GCNGC 7 cut(s) 147, 150, 263, 283, 516, 978, 1127
FokI GGATG 6 cut(s) 39, 45, 281, 894, 1037, 1116
Fsp4HI GCNGC 7 cut(s) 147, 150, 263, 283, 516, 978, 1127
FspBI CTAG 2 cut(s) 111, 1059
GluI GCNGC 7 cut(s) 147, 150, 263, 283, 516, 978, 1127
HaeIII GGCC 1 cut(s) 1576
HapII CCGG 2 cut(s) 968, 1004
HgaI GACGC 1 cut(s) 427
HincII GTYRAC 1 cut(s) 500
HindII GTYRAC 1 cut(s) 500
HinfI GANTC 7 cut(s) 19, 663, 671, 769, 875, 1306, 1505
HpaII CCGG 2 cut(s) 968, 1004
HphI GGTGA 2 cut(s) 806, 1397
Hpy166II GTNNAC 2 cut(s) 500, 1544
Hpy188I TCNGA 8 cut(s) 217, 255, 880, 1180, 1279, 1305, 1480, 1561
Hpy188III TCNNGA 6 cut(s) 314, 413, 766, 1088, 1310, 1424
Hpy8I GTNNAC 2 cut(s) 500, 1544
Hpy99I CGWCG 1 cut(s) 1268
HpyAV CCTTC 2 cut(s) 529, 1056
HpyCH4III ACNGT 5 cut(s) 101, 482, 848, 1100, 1255
HpyCH4IV ACGT 2 cut(s) 676, 1553
HpyF10VI GCNNNNNNNGC 1 cut(s) 545
HpyF3I CTNAG 3 cut(s) 117, 278, 414
HpySE526I ACGT 2 cut(s) 676, 1553
Ksp22I TGATCA 4 cut(s) 199, 1090, 1279, 1426
Kzo9I GATC 9 cut(s) 199, 351, 645, 787, 1064, 1090, 1180, 1279, 1426
LmnI GCTCC 3 cut(s) 31, 229, 545
Lsp1109I GCAGC 7 cut(s) 158, 161, 274, 294, 527, 964, 1113
LweI GCATC 6 cut(s) 67, 259, 1138, 1184, 1245, 1378
MabI ACCWGGT 1 cut(s) 1381
MaeI CTAG 2 cut(s) 111, 1059
MaeII ACGT 2 cut(s) 676, 1553
MaeIII GTNAC 1 cut(s) 79
MalI GATC 9 cut(s) 201, 353, 647, 789, 1066, 1092, 1182, 1281, 1428
MboI GATC 9 cut(s) 199, 351, 645, 787, 1064, 1090, 1180, 1279, 1426
MfeI CAATTG 1 cut(s) 640
MflI RGATCY 2 cut(s) 787, 1064
MhlI GDGCHC 2 cut(s) 726, 1345
MlsI TGGCCA 1 cut(s) 1576
MluCI AATT 7 cut(s) 73, 640, 732, 1199, 1246, 1298, 1449
MluNI TGGCCA 1 cut(s) 1576
MlyI GAGTC 5 cut(s) 28, 672, 763, 884, 1315
MmeI TCCRAC 2 cut(s) 47, 690
Mox20I TGGCCA 1 cut(s) 1576
Mph1103I ATGCAT 1 cut(s) 654
MroXI GAANNNNTTC 1 cut(s) 1566
MscI TGGCCA 1 cut(s) 1576
MseI TTAA 2 cut(s) 243, 393
MslI CAYNNNNRTG 5 cut(s) 165, 180, 833, 1081, 1362
Msp20I TGGCCA 1 cut(s) 1576
MspA1I CMGCKG 2 cut(s) 293, 518
MspI CCGG 2 cut(s) 968, 1004
MspR9I CCNGG 4 cut(s) 792, 968, 969, 1383
MunI CAATTG 1 cut(s) 640
Mva1269I GAATGC 2 cut(s) 1324, 1437
MvaI CCWGG 2 cut(s) 792, 1383
MwoI GCNNNNNNNGC 1 cut(s) 545
NciI CCSGG 2 cut(s) 968, 969
NdeII GATC 9 cut(s) 199, 351, 645, 787, 1064, 1090, 1180, 1279, 1426
NlaIV GGNNCC 2 cut(s) 33, 187
NsiI ATGCAT 1 cut(s) 654
NspI RCATGY 2 cut(s) 997, 1275
NspV TTCGAA 1 cut(s) 71
PctI GAATGC 2 cut(s) 1324, 1437
PdmI GAANNNNTTC 1 cut(s) 1566
PfeI GAWTC 2 cut(s) 671, 1505
PinAI ACCGGT 1 cut(s) 1003
PkrI GCNGC 7 cut(s) 148, 151, 264, 284, 517, 979, 1128
PleI GAGTC 5 cut(s) 27, 671, 763, 883, 1314
PpsI GAGTC 5 cut(s) 27, 671, 763, 883, 1314
Ppu21I YACGTR 2 cut(s) 677, 1554
PsiI TTATAA 2 cut(s) 738, 1020
Psp6I CCWGG 2 cut(s) 790, 1381
PspGI CCWGG 2 cut(s) 790, 1381
PspN4I GGNNCC 2 cut(s) 33, 187
PspPI GGNCC 1 cut(s) 1403
PstI CTGCAG 1 cut(s) 345
PsuI RGATCY 2 cut(s) 787, 1064
PvuII CAGCTG 2 cut(s) 293, 518
RsaI GTAC 4 cut(s) 157, 461, 659, 679
RsaNI GTAC 4 cut(s) 156, 460, 658, 678
RseI CAYNNNNRTG 5 cut(s) 165, 180, 833, 1081, 1362
SaqAI TTAA 2 cut(s) 243, 393
SatI GCNGC 7 cut(s) 147, 150, 263, 283, 516, 978, 1127
Sau3AI GATC 9 cut(s) 199, 351, 645, 787, 1064, 1090, 1180, 1279, 1426
Sau96I GGNCC 1 cut(s) 1403
SchI GAGTC 5 cut(s) 28, 672, 763, 884, 1315
ScrFI CCNGG 4 cut(s) 792, 968, 969, 1383
SduI GDGCHC 2 cut(s) 726, 1345
SexAI ACCWGGT 1 cut(s) 1381
SfaNI GCATC 6 cut(s) 67, 259, 1138, 1184, 1245, 1378
SfcI CTRYAG 5 cut(s) 341, 540, 607, 727, 1052
SfuI TTCGAA 1 cut(s) 71
SinI GGWCC 1 cut(s) 1403
SmaI CCCGGG 1 cut(s) 969
SmiMI CAYNNNNRTG 5 cut(s) 165, 180, 833, 1081, 1362
SnaBI TACGTA 1 cut(s) 1554
SpeI ACTAGT 1 cut(s) 1058
Sse9I AATT 7 cut(s) 73, 640, 732, 1199, 1246, 1298, 1449
SsiI CCGC 3 cut(s) 56, 331, 403
SspMI CTAG 2 cut(s) 111, 1059
StyD4I CCNGG 4 cut(s) 790, 966, 967, 1381
StyI CCWWGG 1 cut(s) 63
TaaI ACNGT 5 cut(s) 101, 482, 848, 1100, 1255
TaiI ACGT 2 cut(s) 679, 1556
TaqI TCGA 3 cut(s) 71, 772, 1378
TasI AATT 7 cut(s) 73, 640, 732, 1199, 1246, 1298, 1449
TfiI GAWTC 2 cut(s) 671, 1505
Tru1I TTAA 2 cut(s) 243, 393
Tru9I TTAA 2 cut(s) 243, 393
TscAI CASTG 4 cut(s) 106, 129, 1083, 1419
TseI GCWGC 7 cut(s) 146, 149, 262, 282, 515, 977, 1126
TspDTI ATGAA 4 cut(s) 156, 333, 857, 1530
TspGWI ACGGA 3 cut(s) 845, 887, 1560
TspMI CCCGGG 1 cut(s) 967
TspRI CASTG 4 cut(s) 106, 129, 1083, 1419
VpaK11BI GGWCC 1 cut(s) 1403
XapI RAATTY 3 cut(s) 73, 732, 1246
XceI RCATGY 2 cut(s) 997, 1275
XcmI CCANNNNNNNNNTGG 1 cut(s) 382
XmaI CCCGGG 1 cut(s) 967
XmnI GAANNNNTTC 1 cut(s) 1566
XspI CTAG 2 cut(s) 111, 1059
Zsp2I ATGCAT 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.