Rw5G032470
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014699
Physical Location & Seq
Forward (+)
54984416 .. 54986993
2578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G032470.1

Sequence Viewer

Length: 1659 bp
ATGTCTTCTCCCTCAAAGAGTCCAACTATGGAGCTTGTTCAGCAAAGTCATCCGCATCCCAAGGCTTTTGTTACCCAAGTTACTGCTTACGGCGGTAGCCCTAGTCCTATAAAGCAACTGCAACCAATTCCAAATGAAGCTGATCGCAAGGCTTCTTTGACCGGGACGCATGATGCCATTGTTGTGTCCTCTGTTGCAGCAGAGGTCGATCAGTTTTCGGACGTGGAAGAGGAGCTGAACAATTTCAATAACAATCAGAAAAGGCAGCAGGATGCTTTTCTGAACAGTTTTCCACCTGGGTATAGGTTCAATCCTCGTGATGACGAACTTGTTCGGTATTACCTGCAGAAGAAGATCATGGACGAGCCATTGCCCCCCAACAAGATCATGGAAGTTAACCTCTACCGCCACAATCCCGAGACGCTTGCTGAAAGATATGAAACTTACGGAGAAAAAGAATGGTACTTCTTTACTCCAAGGGACCGGAAGTATCGCAATGGATTTCGACCAAATAGGGCAGCTGGTGATGGATATTGGAAGGCCACAGGAGCTGACAAAACAATAAGGTCTGGCAATGTAGAGGTTGGGTGTAGAAAGGCTCTCGTTTTTTACAGGGGTAAACCTCCAAAGGGTAAAAAGACGAATTGGATCATGCATGAGTATCGAGTCCCTGATTCCCCTGATTCTCGTCCTCCCAAAAGGACTGAAAATGACATGAGGTTGGATAACTGGGTGCTGTGTAGAATTTATAAGAAAGCTGATAACAAGAACAACAAAAGCCAGAGCCGAAATCATGTAGAGGATCCTGTTGAAGAAAGTATCTCTACATCACTACCAGATAACATGGACATGGAGATGGAGACGAACGGTCAAGTTGATAACCCTCAAGCATTGGAGTCTGAAAAGGCATACCCTTACCCTATTGTTCATCCACCATTGGAGTTTGACAGGGCGTATCCTAATTATGCATTTCCTTACCCGGGTGGGCGGCCTCTATGTGCTCTAGTTAACAACAATATGAACAACATGCTTCATCACCACCGATTCCAACCAGTCTCTTTGAATTCTGGGGATAGTTATGCAAATATGGGGCAAATGCAACAGGCTTGTGGCACTTATTCACCAATGCAATTTCCTGATCAAAACTGTGGTGTGCAATTTTCTGATCAAAAGCGTGGTGTGCAATTTTCGGACCAAAGGTTTATTATGCAACCAGTTGATGTCACATCAGGATCAATGGTATGTACAGATGATCAAAAGTGTGGTGTCCAATTTCCAGACGAAAAGTTTATTATGCAATCACTTGATGTCACATCAGAATCAATGGTATGTACAGATGAGGCATGTTCTGATCAGAAATGTGGTAGGCAATTTCCGGATCAAAAATTTATTGTGCAATCACCTGATGGTAGCCCACAACCAATGACATTTTCTCATGAAAAAAGTGGAAAGAGTGGCCAACCAGATGATGCAGTTGATAGTAGACCATCATCAGTGCCTCCTTTACCTGATCAAAAGAGTACTCAACAAGGAATTGGTAAGCCTCTATTACCACCATCTGATCAAGGTCTACGATACGTAGAAGAATCGAATGAAGATCATAATCTCATGTTTGGTTATCCACCATTTGTGTCTTTGGAAACTTTTTCATGGCCCTGA

Protein Analysis

552

Amino Acids

62.71

Weight (kDa)

5.58

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 98 - 223 1.9e-44 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 750
Acc36I ACCTGC 1 cut(s) 351
AccI GTMKAC 2 cut(s) 1483, 1570
AccIII TCCGGA 1 cut(s) 1375
AciI CCGC 4 cut(s) 53, 93, 406, 988
AclWI GGATC 5 cut(s) 656, 797, 810, 1240, 1386
AcoI YGGCCR 1 cut(s) 1456
AcsI RAATTY 3 cut(s) 744, 1063, 1385
AfaI GTAC 4 cut(s) 464, 1246, 1333, 1522
AfiI CCNNNNNNNGG 2 cut(s) 629, 980
AgsI TTSAA 4 cut(s) 247, 310, 812, 1063
AhdI GACNNNNNGTC 1 cut(s) 867
AjiI CACGTC 1 cut(s) 223
AjnI CCWGG 1 cut(s) 295
AjuI GAANNNNNNNTTGG 2 cut(s) 1414, 1446
AluBI AGCT 6 cut(s) 34, 140, 235, 521, 551, 758
AluI AGCT 6 cut(s) 34, 140, 235, 521, 551, 758
Alw21I GWGCWC 1 cut(s) 1003
Alw26I GTCTC 3 cut(s) 413, 854, 1060
AlwI GGATC 5 cut(s) 656, 797, 810, 1240, 1386
AlwNI CAGNNNCTG 1 cut(s) 551
Ama87I CYCGRG 2 cut(s) 416, 979
Aor13HI TCCGGA 1 cut(s) 1375
AoxI GGCC 4 cut(s) 540, 989, 1456, 1652
ApeKI GCWGC 3 cut(s) 197, 265, 518
ApoI RAATTY 3 cut(s) 744, 1063, 1385
Asp700I GAANNNNTTC 2 cut(s) 242, 330
AspS9I GGNCC 3 cut(s) 481, 1192, 1653
AsuC2I CCSGG 3 cut(s) 163, 980, 981
AsuHPI GGTGA 4 cut(s) 536, 1028, 1113, 1392
AvaI CYCGRG 2 cut(s) 416, 979
AvaII GGWCC 2 cut(s) 481, 1192
BalI TGGCCA 1 cut(s) 1458
BamHI GGATCC 1 cut(s) 802
BauI CACGAG 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 1003
BbvI GCAGC 3 cut(s) 209, 277, 530
BccI CCATC 5 cut(s) 521, 850, 1400, 1495, 1564
BceAI ACGGC 1 cut(s) 106
BcgI CGANNNNNNTGC 4 cut(s) 407, 441, 644, 678
BciT130I CCWGG 1 cut(s) 297
BciVI GTATCC 1 cut(s) 966
BclI TGATCA 6 cut(s) 1138, 1165, 1252, 1351, 1510, 1561
BcnI CCSGG 3 cut(s) 163, 980, 981
BcoDI GTCTC 3 cut(s) 413, 854, 1060
BfaI CTAG 2 cut(s) 102, 1004
BfmI CTRYAG 1 cut(s) 344
BfuAI ACCTGC 1 cut(s) 351
BfuI GTATCC 1 cut(s) 966
BisI GCNGC 4 cut(s) 198, 266, 519, 989
BlsI GCNGC 4 cut(s) 199, 267, 520, 990
BmcAI AGTACT 1 cut(s) 1522
Bme1390I CCNGG 4 cut(s) 163, 297, 980, 981
Bme18I GGWCC 2 cut(s) 481, 1192
BmeRI GACNNNNNGTC 1 cut(s) 867
BmeT110I CYCGRG 2 cut(s) 416, 979
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 3 cut(s) 481, 1192, 1653
BmiI GGNNCC 2 cut(s) 482, 804
BmrFI CCNGG 4 cut(s) 163, 297, 980, 981
BmrI ACTGGG 1 cut(s) 739
BmsI GCATC 4 cut(s) 64, 163, 262, 1459
BmuI ACTGGG 1 cut(s) 739
BpuEI CTTGAG 1 cut(s) 870
BpuMI CCSGG 3 cut(s) 163, 980, 981
BsaAI YACGTR 1 cut(s) 1579
BsaBI GATNNNNATC 1 cut(s) 1602
BsaJI CCNNGG 4 cut(s) 60, 296, 476, 979
BsaWI WCCGGW 2 cut(s) 483, 1375
Bsc4I CCNNNNNNNGG 2 cut(s) 629, 980
Bse1I ACTGG 3 cut(s) 734, 1052, 1214
Bse3DI GCAATG 3 cut(s) 368, 502, 580
Bse8I GATNNNNATC 1 cut(s) 1602
BseAI TCCGGA 1 cut(s) 1375
BseBI CCWGG 1 cut(s) 297
BseDI CCNNGG 4 cut(s) 60, 296, 476, 979
BseGI GGATG 4 cut(s) 49, 55, 277, 928
BseJI GATNNNNATC 1 cut(s) 1602
BseLI CCNNNNNNNGG 2 cut(s) 629, 980
BseMI GCAATG 3 cut(s) 368, 502, 580
BseNI ACTGG 3 cut(s) 734, 1052, 1214
BseRI GAGGAG 1 cut(s) 245
BseXI GCAGC 3 cut(s) 209, 277, 530
BshFI GGCC 4 cut(s) 542, 991, 1458, 1654
BsiHKAI GWGCWC 1 cut(s) 1003
BsiHKCI CYCGRG 2 cut(s) 416, 979
BsiSI CCGG 4 cut(s) 162, 484, 980, 1376
BslFI GGGAC 3 cut(s) 178, 494, 653
BslI CCNNNNNNNGG 2 cut(s) 629, 980
BsmAI GTCTC 3 cut(s) 413, 854, 1060
BsmBI CGTCTC 2 cut(s) 413, 854
BsmFI GGGAC 3 cut(s) 178, 494, 653
BsnI GGCC 4 cut(s) 542, 991, 1458, 1654
BsoBI CYCGRG 2 cut(s) 416, 979
Bsp1286I GDGCHC 1 cut(s) 1003
Bsp13I TCCGGA 1 cut(s) 1375
Bsp1407I TGTACA 2 cut(s) 1244, 1331
BspACI CCGC 4 cut(s) 53, 93, 406, 988
BspANI GGCC 4 cut(s) 542, 991, 1458, 1654
BspEI TCCGGA 1 cut(s) 1375
BspHI TCATGA 1 cut(s) 1435
BspLI GGNNCC 2 cut(s) 482, 804
BspMAI CTGCAG 1 cut(s) 348
BspMI ACCTGC 1 cut(s) 351
BspPI GGATC 5 cut(s) 656, 797, 810, 1240, 1386
BsrDI GCAATG 3 cut(s) 368, 502, 580
BsrGI TGTACA 2 cut(s) 1244, 1331
BsrI ACTGG 3 cut(s) 734, 1052, 1214
BssECI CCNNGG 4 cut(s) 60, 296, 476, 979
BssSI CACGAG 1 cut(s) 315
BssT1I CCWWGG 2 cut(s) 60, 476
Bst2BI CACGAG 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 297
Bst4CI ACNGT 3 cut(s) 287, 869, 1148
Bst6I CTCTTC 1 cut(s) 222
BstAUI TGTACA 2 cut(s) 1244, 1331
BstBAI YACGTR 1 cut(s) 1579
BstC8I GCNNGC 1 cut(s) 426
BstF5I GGATG 4 cut(s) 49, 55, 277, 928
BstMAI GTCTC 3 cut(s) 413, 854, 1060
BstMWI GCNNNNNNNGC 3 cut(s) 40, 548, 1180
BstNI CCWGG 1 cut(s) 297
BstNSI RCATGY 2 cut(s) 1030, 1347
BstSCI CCNGG 4 cut(s) 161, 295, 978, 979
BstSFI CTRYAG 1 cut(s) 344
BstSNI TACGTA 1 cut(s) 1579
BstV1I GCAGC 3 cut(s) 209, 277, 530
BstX2I RGATCY 1 cut(s) 802
BstYI RGATCY 1 cut(s) 802
BsuI GTATCC 1 cut(s) 966
BsuRI GGCC 4 cut(s) 542, 991, 1458, 1654
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 4 cut(s) 49, 55, 277, 928
BtsIMutI CAGTG 1 cut(s) 1500
BveI ACCTGC 1 cut(s) 351
Cac8I GCNNGC 1 cut(s) 426
CaiI CAGNNNCTG 1 cut(s) 551
CciI TCATGA 1 cut(s) 1435
Cfr13I GGNCC 3 cut(s) 481, 1192, 1653
Cfr9I CCCGGG 1 cut(s) 979
CseI GACGC 2 cut(s) 175, 430
Csp6I GTAC 4 cut(s) 463, 1245, 1332, 1521
CviQI GTAC 4 cut(s) 463, 1245, 1332, 1521
DriI GACNNNNNGTC 1 cut(s) 867
EaeI YGGCCR 1 cut(s) 1456
Eam1104I CTCTTC 1 cut(s) 222
Eam1105I GACNNNNNGTC 1 cut(s) 867
EarI CTCTTC 1 cut(s) 222
Eco105I TACGTA 1 cut(s) 1579
Eco130I CCWWGG 2 cut(s) 60, 476
Eco47I GGWCC 2 cut(s) 481, 1192
Eco88I CYCGRG 2 cut(s) 416, 979
EcoRI GAATTC 1 cut(s) 1063
EcoRII CCWGG 1 cut(s) 295
EcoT14I CCWWGG 2 cut(s) 60, 476
EcoT22I ATGCAT 2 cut(s) 657, 970
ErhI CCWWGG 2 cut(s) 60, 476
Esp3I CGTCTC 2 cut(s) 413, 854
FaqI GGGAC 3 cut(s) 178, 494, 653
FbaI TGATCA 6 cut(s) 1138, 1165, 1252, 1351, 1510, 1561
FblI GTMKAC 2 cut(s) 1483, 1570
Fnu4HI GCNGC 4 cut(s) 198, 266, 519, 989
FokI GGATG 4 cut(s) 36, 42, 284, 915
Fsp4HI GCNGC 4 cut(s) 198, 266, 519, 989
FspBI CTAG 2 cut(s) 102, 1004
GluI GCNGC 4 cut(s) 198, 266, 519, 989
HaeIII GGCC 4 cut(s) 542, 991, 1458, 1654
HapII CCGG 4 cut(s) 162, 484, 980, 1376
HgaI GACGC 2 cut(s) 175, 430
HincII GTYRAC 2 cut(s) 397, 1009
HindII GTYRAC 2 cut(s) 397, 1009
HinfI GANTC 8 cut(s) 19, 666, 674, 683, 896, 1044, 1319, 1586
HpaI GTTAAC 2 cut(s) 397, 1009
HpaII CCGG 4 cut(s) 162, 484, 980, 1376
HphI GGTGA 4 cut(s) 536, 1028, 1113, 1392
Hpy166II GTNNAC 5 cut(s) 397, 620, 1009, 1484, 1571
Hpy188III TCNNGA 7 cut(s) 317, 416, 1136, 1230, 1277, 1376, 1436
Hpy8I GTNNAC 5 cut(s) 397, 620, 1009, 1484, 1571
HpyAV CCTTC 1 cut(s) 532
HpyCH4III ACNGT 3 cut(s) 287, 869, 1148
HpyCH4IV ACGT 2 cut(s) 222, 1578
HpyF10VI GCNNNNNNNGC 3 cut(s) 40, 548, 1180
HpySE526I ACGT 2 cut(s) 222, 1578
Kpn2I TCCGGA 1 cut(s) 1375
Ksp22I TGATCA 6 cut(s) 1138, 1165, 1252, 1351, 1510, 1561
KspAI GTTAAC 2 cut(s) 397, 1009
LmnI GCTCC 3 cut(s) 31, 232, 548
Lsp1109I GCAGC 3 cut(s) 209, 277, 530
LweI GCATC 4 cut(s) 64, 163, 262, 1459
MaeI CTAG 2 cut(s) 102, 1004
MaeII ACGT 2 cut(s) 222, 1578
MaeIII GTNAC 4 cut(s) 70, 79, 1222, 1309
MboII GAAGA 6 cut(s) 239, 361, 364, 824, 1595, 1607
MflI RGATCY 1 cut(s) 802
MhlI GDGCHC 1 cut(s) 1003
MlsI TGGCCA 1 cut(s) 1458
MluNI TGGCCA 1 cut(s) 1458
MlyI GAGTC 3 cut(s) 28, 675, 905
MmeI TCCRAC 3 cut(s) 47, 702, 1072
Mox20I TGGCCA 1 cut(s) 1458
Mph1103I ATGCAT 2 cut(s) 657, 970
MroI TCCGGA 1 cut(s) 1375
MroXI GAANNNNTTC 2 cut(s) 242, 330
MscI TGGCCA 1 cut(s) 1458
MseI TTAA 2 cut(s) 396, 1008
MslI CAYNNNNRTG 3 cut(s) 182, 848, 854
Msp20I TGGCCA 1 cut(s) 1458
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 4 cut(s) 162, 484, 980, 1376
MspR9I CCNGG 4 cut(s) 163, 297, 980, 981
MvaI CCWGG 1 cut(s) 297
MwoI GCNNNNNNNGC 3 cut(s) 40, 548, 1180
NciI CCSGG 3 cut(s) 163, 980, 981
NlaIV GGNNCC 2 cut(s) 482, 804
NmuCI GTSAC 2 cut(s) 1222, 1309
NsiI ATGCAT 2 cut(s) 657, 970
NspI RCATGY 2 cut(s) 1030, 1347
PagI TCATGA 1 cut(s) 1435
PdmI GAANNNNTTC 2 cut(s) 242, 330
PfeI GAWTC 5 cut(s) 674, 683, 1044, 1319, 1586
PkrI GCNGC 4 cut(s) 199, 267, 520, 990
PleI GAGTC 3 cut(s) 27, 674, 904
PpsI GAGTC 3 cut(s) 27, 674, 904
Ppu21I YACGTR 1 cut(s) 1579
PsiI TTATAA 1 cut(s) 750
Psp6I CCWGG 1 cut(s) 295
PspGI CCWGG 1 cut(s) 295
PspN4I GGNNCC 2 cut(s) 482, 804
PspPI GGNCC 3 cut(s) 481, 1192, 1653
PstI CTGCAG 1 cut(s) 348
PstNI CAGNNNCTG 1 cut(s) 551
PsuI RGATCY 1 cut(s) 802
PvuII CAGCTG 1 cut(s) 521
RsaI GTAC 4 cut(s) 464, 1246, 1333, 1522
RsaNI GTAC 4 cut(s) 463, 1245, 1332, 1521
RseI CAYNNNNRTG 3 cut(s) 182, 848, 854
SaqAI TTAA 2 cut(s) 396, 1008
SatI GCNGC 4 cut(s) 198, 266, 519, 989
Sau96I GGNCC 3 cut(s) 481, 1192, 1653
ScaI AGTACT 1 cut(s) 1522
SchI GAGTC 3 cut(s) 28, 675, 905
ScrFI CCNGG 4 cut(s) 163, 297, 980, 981
SduI GDGCHC 1 cut(s) 1003
SfaNI GCATC 4 cut(s) 64, 163, 262, 1459
SfcI CTRYAG 1 cut(s) 344
SinI GGWCC 2 cut(s) 481, 1192
SmaI CCCGGG 1 cut(s) 981
SmiMI CAYNNNNRTG 3 cut(s) 182, 848, 854
SmlI CTYRAG 1 cut(s) 885
SmoI CTYRAG 1 cut(s) 885
SnaBI TACGTA 1 cut(s) 1579
SsiI CCGC 4 cut(s) 53, 93, 406, 988
SspMI CTAG 2 cut(s) 102, 1004
StyD4I CCNGG 4 cut(s) 161, 295, 978, 979
StyI CCWWGG 2 cut(s) 60, 476
TaaI ACNGT 3 cut(s) 287, 869, 1148
TaiI ACGT 2 cut(s) 225, 1581
TaqI TCGA 4 cut(s) 207, 505, 664, 1589
TatI WGTACW 3 cut(s) 1244, 1331, 1520
TauI GCSGC 1 cut(s) 991
TfiI GAWTC 5 cut(s) 674, 683, 1044, 1319, 1586
Tru1I TTAA 2 cut(s) 396, 1008
Tru9I TTAA 2 cut(s) 396, 1008
TscAI CASTG 1 cut(s) 1500
TseFI GTSAC 2 cut(s) 1222, 1309
TseI GCWGC 3 cut(s) 197, 265, 518
Tsp45I GTSAC 2 cut(s) 1222, 1309
TspDTI ATGAA 8 cut(s) 150, 453, 917, 1022, 1034, 1452, 1608, 1638
TspGWI ACGGA 1 cut(s) 462
TspMI CCCGGG 1 cut(s) 979
TspRI CASTG 1 cut(s) 1500
VpaK11BI GGWCC 2 cut(s) 481, 1192
XapI RAATTY 3 cut(s) 744, 1063, 1385
XceI RCATGY 2 cut(s) 1030, 1347
XcmI CCANNNNNNNNNTGG 1 cut(s) 385
XmaI CCCGGG 1 cut(s) 979
XmiI GTMKAC 2 cut(s) 1483, 1570
XmnI GAANNNNTTC 2 cut(s) 242, 330
XspI CTAG 2 cut(s) 102, 1004
ZrmI AGTACT 1 cut(s) 1522
Zsp2I ATGCAT 2 cut(s) 657, 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.