Rroxscaffold_1G00028470
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014699
Physical Location & Seq
Reverse (-)
36263998 .. 36266317
2320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00028470.1

Sequence Viewer

Length: 1632 bp
ATGGAGCTTGTTCAGCAAAGTCATCCGCATCCCAAGGTTTCTGTTACCCAAGTTACTGCTTACGGCGGTAGCCCTAGTCCTATAAAGCAACTGCAACCAATTCCAAATGAAGCTGATCGCAAGGCTTCTTTAACCGGGACGCATGATGCCATTGTTGTGTCCTCTGTTGCAGCAGAGGTCGATCAGTTTTCGGACGTGGAAGAGGAGCTGAACAATTTCAATAACAATCAGAAAAGGCAGCAGGATGCTTTTCTGAACAGTTTTCCACCTGGGTATAGGTTCAATCCTCGTGATGACGAACTTGTTCGGTATTACCTGCAGAAGAAGATCATGGATGAGCCATTGCCTCCCAACAAGATCATGGAAGTTAACCTCTACCGCCACAATCCCGAGACGCTTGCTGAAAGATATGAAACTTATGGAGAAAAAGAATGGTACTTCTTTACTCCAAGGGACCGCAAGTATCGCAATGGATTTCGACCAAATAGGGCAGCTGGTGATGGATATTGGAAGGCCACAGGAGCTGACAAAACAATAAGGTCTGGCAATGTAGAGGTTGGGTGTAGAAAGGCTCTCGTTTTTTACAGAGGTAAACCTCCAAAGGGTAAAAAGACGAATTGGATCATGCATGAGTATCGAGTCCCTGATTCCCCTGATTCTCGTCCTCCGAAAAGGACTGAAAATGACATGAGGTTGGATAACTGGGTGCTGTGTAGAATTTATAAGAAAGCTGATAATAAGAACAACAAAAGCCAGAGCCGAAATCATGTAGAGGATCCTGTTGAAGAAAGTATCTCTACATCACTACCAGATAACATGGACATGGAGATGGAGATGAACGGTCAAGTTGATAACCCTCAAGCATTGGAGTCCGAAAAGGCATACCATTACCCTATTGTTCATCCACCATTGGAGTTTGACAGGGCGTATCCTAATTATGCATTTCCTTACCCGGGTGGGCGGCCTCTATGTGCTCTAGTTAACAACAATATGAACAACATGCTTCCTCACCACCGATTCCAACCAGTCTCTTTGAATTCTGGGGATAGTTATGCAAATATGGGGCAAATGCCACAGGCTTGTGGCACTTATTCACCTATGCAATTTCCTGATCAAAACTGTGGTGTGCAATTTTCTGATCAAAAGCGTGGTGTGCAATTTTCGGACCAAAGGTTTAGCATGCAACCAGTTGATGTCACATCAGAATCAATGGTATGTACAGATGATCAAAAGTGTGGTGTCCAATTTCCAGATGAAAAGTTTATTATGCAATCACTTGATGTCACATCAGAATCAATGGTATGTACAAATGAGGCATGTTCTGATCAGAAATGTGGTAGGCAATTTCCGGATCAAAAATTTATTGTGCAATCACCTGATGTTAGCCCACAACCAATGACATTTTCTATTGGAAAAAGTGGAAAGAGTGTCCAACCAGATGATGCAGTTGATAGCAGATCTTCATCAGTGCCTTCTTTACCTGATCAAAAGAGTACTCAACACGGAATTGGTAAGCCTCTATTACCACCATCTGATCAAGGTCTACGATACGTAGAAGAATCAAATGAAGATCATAATCTCATGTTTGGTTATCCACCATTTGCGTCTTTGGAAACTTTTTCATGGCCCTGA

Protein Analysis

543

Amino Acids

61.77

Weight (kDa)

5.58

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 89 - 214 1.8e-44 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 723
Acc36I ACCTGC 1 cut(s) 324
AccI GTMKAC 1 cut(s) 1543
AccIII TCCGGA 1 cut(s) 1348
AciI CCGC 5 cut(s) 26, 66, 379, 457, 961
AclWI GGATC 4 cut(s) 629, 770, 783, 1359
AcsI RAATTY 3 cut(s) 717, 1036, 1358
AfaI GTAC 4 cut(s) 437, 1219, 1306, 1495
AfiI CCNNNNNNNGG 2 cut(s) 602, 953
AgsI TTSAA 4 cut(s) 220, 283, 785, 1036
AjiI CACGTC 1 cut(s) 196
AjnI CCWGG 1 cut(s) 268
AjuI GAANNNNNNNTTGG 2 cut(s) 1387, 1419
AluBI AGCT 6 cut(s) 7, 113, 208, 494, 524, 731
AluI AGCT 6 cut(s) 7, 113, 208, 494, 524, 731
Alw21I GWGCWC 1 cut(s) 976
Alw26I GTCTC 2 cut(s) 386, 1033
AlwI GGATC 4 cut(s) 629, 770, 783, 1359
AlwNI CAGNNNCTG 1 cut(s) 524
Ama87I CYCGRG 2 cut(s) 389, 952
Aor13HI TCCGGA 1 cut(s) 1348
AoxI GGCC 3 cut(s) 513, 962, 1625
ApeKI GCWGC 3 cut(s) 170, 238, 491
ApoI RAATTY 3 cut(s) 717, 1036, 1358
Asp700I GAANNNNTTC 2 cut(s) 215, 303
AspS9I GGNCC 3 cut(s) 454, 1165, 1626
AsuC2I CCSGG 3 cut(s) 136, 953, 954
AsuHPI GGTGA 4 cut(s) 509, 1001, 1086, 1365
AvaI CYCGRG 2 cut(s) 389, 952
AvaII GGWCC 2 cut(s) 454, 1165
BamHI GGATCC 1 cut(s) 775
BauI CACGAG 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 976
BbvI GCAGC 3 cut(s) 182, 250, 503
BccI CCATC 3 cut(s) 494, 823, 1537
BceAI ACGGC 1 cut(s) 79
BcgI CGANNNNNNTGC 4 cut(s) 380, 414, 617, 651
BciT130I CCWGG 1 cut(s) 270
BciVI GTATCC 1 cut(s) 939
BclI TGATCA 6 cut(s) 1111, 1138, 1225, 1324, 1483, 1534
BcnI CCSGG 3 cut(s) 136, 953, 954
BcoDI GTCTC 2 cut(s) 386, 1033
BfaI CTAG 2 cut(s) 75, 977
BfmI CTRYAG 1 cut(s) 317
BfuAI ACCTGC 1 cut(s) 324
BfuI GTATCC 1 cut(s) 939
BglII AGATCT 1 cut(s) 1457
BisI GCNGC 4 cut(s) 171, 239, 492, 962
BlsI GCNGC 4 cut(s) 172, 240, 493, 963
BmcAI AGTACT 1 cut(s) 1495
Bme1390I CCNGG 4 cut(s) 136, 270, 953, 954
Bme18I GGWCC 2 cut(s) 454, 1165
BmeT110I CYCGRG 2 cut(s) 389, 952
BmgBI CACGTC 1 cut(s) 196
BmgT120I GGNCC 3 cut(s) 454, 1165, 1626
BmiI GGNNCC 2 cut(s) 455, 777
BmrFI CCNGG 4 cut(s) 136, 270, 953, 954
BmrI ACTGGG 1 cut(s) 712
BmsI GCATC 4 cut(s) 37, 136, 235, 1432
BmuI ACTGGG 1 cut(s) 712
BpuEI CTTGAG 1 cut(s) 843
BpuMI CCSGG 3 cut(s) 136, 953, 954
BsaAI YACGTR 1 cut(s) 1552
BsaBI GATNNNNATC 2 cut(s) 1462, 1575
BsaJI CCNNGG 4 cut(s) 33, 269, 449, 952
BsaWI WCCGGW 1 cut(s) 1348
Bsc4I CCNNNNNNNGG 2 cut(s) 602, 953
Bse1I ACTGG 3 cut(s) 707, 1025, 1187
Bse3DI GCAATG 3 cut(s) 341, 475, 553
Bse8I GATNNNNATC 2 cut(s) 1462, 1575
BseAI TCCGGA 1 cut(s) 1348
BseBI CCWGG 1 cut(s) 270
BseDI CCNNGG 4 cut(s) 33, 269, 449, 952
BseGI GGATG 5 cut(s) 22, 28, 250, 340, 901
BseJI GATNNNNATC 2 cut(s) 1462, 1575
BseLI CCNNNNNNNGG 2 cut(s) 602, 953
BseMI GCAATG 3 cut(s) 341, 475, 553
BseNI ACTGG 3 cut(s) 707, 1025, 1187
BseRI GAGGAG 1 cut(s) 218
BseXI GCAGC 3 cut(s) 182, 250, 503
BshFI GGCC 3 cut(s) 515, 964, 1627
BsiHKAI GWGCWC 1 cut(s) 976
BsiHKCI CYCGRG 2 cut(s) 389, 952
BsiSI CCGG 3 cut(s) 135, 953, 1349
BslFI GGGAC 3 cut(s) 151, 467, 626
BslI CCNNNNNNNGG 2 cut(s) 602, 953
BsmAI GTCTC 2 cut(s) 386, 1033
BsmBI CGTCTC 1 cut(s) 386
BsmFI GGGAC 3 cut(s) 151, 467, 626
BsnI GGCC 3 cut(s) 515, 964, 1627
BsoBI CYCGRG 2 cut(s) 389, 952
Bsp1286I GDGCHC 1 cut(s) 976
Bsp13I TCCGGA 1 cut(s) 1348
Bsp1407I TGTACA 2 cut(s) 1217, 1304
BspACI CCGC 5 cut(s) 26, 66, 379, 457, 961
BspANI GGCC 3 cut(s) 515, 964, 1627
BspEI TCCGGA 1 cut(s) 1348
BspLI GGNNCC 2 cut(s) 455, 777
BspMAI CTGCAG 1 cut(s) 321
BspMI ACCTGC 1 cut(s) 324
BspPI GGATC 4 cut(s) 629, 770, 783, 1359
BsrDI GCAATG 3 cut(s) 341, 475, 553
BsrGI TGTACA 2 cut(s) 1217, 1304
BsrI ACTGG 3 cut(s) 707, 1025, 1187
BssECI CCNNGG 4 cut(s) 33, 269, 449, 952
BssSI CACGAG 1 cut(s) 288
BssT1I CCWWGG 2 cut(s) 33, 449
Bst2BI CACGAG 1 cut(s) 288
Bst2UI CCWGG 1 cut(s) 270
Bst4CI ACNGT 3 cut(s) 260, 842, 1121
Bst6I CTCTTC 1 cut(s) 195
BstAUI TGTACA 2 cut(s) 1217, 1304
BstBAI YACGTR 1 cut(s) 1552
BstC8I GCNNGC 2 cut(s) 399, 1181
BstF5I GGATG 5 cut(s) 22, 28, 250, 340, 901
BstMAI GTCTC 2 cut(s) 386, 1033
BstMWI GCNNNNNNNGC 4 cut(s) 13, 465, 521, 1153
BstNI CCWGG 1 cut(s) 270
BstNSI RCATGY 3 cut(s) 1003, 1183, 1320
BstSCI CCNGG 4 cut(s) 134, 268, 951, 952
BstSFI CTRYAG 1 cut(s) 317
BstSNI TACGTA 1 cut(s) 1552
BstV1I GCAGC 3 cut(s) 182, 250, 503
BstX2I RGATCY 2 cut(s) 775, 1457
BstYI RGATCY 2 cut(s) 775, 1457
BsuI GTATCC 1 cut(s) 939
BsuRI GGCC 3 cut(s) 515, 964, 1627
BtrI CACGTC 1 cut(s) 196
BtsCI GGATG 5 cut(s) 22, 28, 250, 340, 901
BtsIMutI CAGTG 1 cut(s) 1473
BveI ACCTGC 1 cut(s) 324
Cac8I GCNNGC 2 cut(s) 399, 1181
CaiI CAGNNNCTG 1 cut(s) 524
Cfr13I GGNCC 3 cut(s) 454, 1165, 1626
Cfr9I CCCGGG 1 cut(s) 952
CseI GACGC 3 cut(s) 148, 403, 1593
Csp6I GTAC 4 cut(s) 436, 1218, 1305, 1494
CviQI GTAC 4 cut(s) 436, 1218, 1305, 1494
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
Eco105I TACGTA 1 cut(s) 1552
Eco130I CCWWGG 2 cut(s) 33, 449
Eco47I GGWCC 2 cut(s) 454, 1165
Eco88I CYCGRG 2 cut(s) 389, 952
EcoRI GAATTC 1 cut(s) 1036
EcoRII CCWGG 1 cut(s) 268
EcoT14I CCWWGG 2 cut(s) 33, 449
EcoT22I ATGCAT 2 cut(s) 630, 943
ErhI CCWWGG 2 cut(s) 33, 449
Esp3I CGTCTC 1 cut(s) 386
FaqI GGGAC 3 cut(s) 151, 467, 626
FbaI TGATCA 6 cut(s) 1111, 1138, 1225, 1324, 1483, 1534
FblI GTMKAC 1 cut(s) 1543
Fnu4HI GCNGC 4 cut(s) 171, 239, 492, 962
FokI GGATG 5 cut(s) 9, 15, 257, 347, 888
Fsp4HI GCNGC 4 cut(s) 171, 239, 492, 962
FspBI CTAG 2 cut(s) 75, 977
GluI GCNGC 4 cut(s) 171, 239, 492, 962
HaeIII GGCC 3 cut(s) 515, 964, 1627
HapII CCGG 3 cut(s) 135, 953, 1349
HgaI GACGC 3 cut(s) 148, 403, 1593
HincII GTYRAC 2 cut(s) 370, 982
HindII GTYRAC 2 cut(s) 370, 982
HinfI GANTC 8 cut(s) 639, 647, 656, 869, 1017, 1205, 1292, 1559
HpaI GTTAAC 2 cut(s) 370, 982
HpaII CCGG 3 cut(s) 135, 953, 1349
HphI GGTGA 4 cut(s) 509, 1001, 1086, 1365
Hpy166II GTNNAC 4 cut(s) 370, 593, 982, 1544
Hpy188III TCNNGA 5 cut(s) 290, 389, 1109, 1250, 1349
Hpy8I GTNNAC 4 cut(s) 370, 593, 982, 1544
HpyAV CCTTC 2 cut(s) 505, 1482
HpyCH4III ACNGT 3 cut(s) 260, 842, 1121
HpyCH4IV ACGT 2 cut(s) 195, 1551
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 465, 521, 1153
HpySE526I ACGT 2 cut(s) 195, 1551
Kpn2I TCCGGA 1 cut(s) 1348
Ksp22I TGATCA 6 cut(s) 1111, 1138, 1225, 1324, 1483, 1534
KspAI GTTAAC 2 cut(s) 370, 982
LmnI GCTCC 3 cut(s) 4, 205, 521
Lsp1109I GCAGC 3 cut(s) 182, 250, 503
LweI GCATC 4 cut(s) 37, 136, 235, 1432
MaeI CTAG 2 cut(s) 75, 977
MaeII ACGT 2 cut(s) 195, 1551
MaeIII GTNAC 4 cut(s) 43, 52, 1195, 1282
MboII GAAGA 7 cut(s) 212, 334, 337, 797, 1452, 1568, 1580
MflI RGATCY 2 cut(s) 775, 1457
MhlI GDGCHC 1 cut(s) 976
MlyI GAGTC 2 cut(s) 648, 878
MmeI TCCRAC 3 cut(s) 675, 1045, 1456
Mph1103I ATGCAT 2 cut(s) 630, 943
MroI TCCGGA 1 cut(s) 1348
MroXI GAANNNNTTC 2 cut(s) 215, 303
MseI TTAA 3 cut(s) 131, 369, 981
MslI CAYNNNNRTG 3 cut(s) 155, 821, 827
MspA1I CMGCKG 1 cut(s) 494
MspI CCGG 3 cut(s) 135, 953, 1349
MspR9I CCNGG 4 cut(s) 136, 270, 953, 954
MvaI CCWGG 1 cut(s) 270
MwoI GCNNNNNNNGC 4 cut(s) 13, 465, 521, 1153
NciI CCSGG 3 cut(s) 136, 953, 954
NlaIV GGNNCC 2 cut(s) 455, 777
NmuCI GTSAC 2 cut(s) 1195, 1282
NsiI ATGCAT 2 cut(s) 630, 943
NspI RCATGY 3 cut(s) 1003, 1183, 1320
PaeI GCATGC 1 cut(s) 1183
PdmI GAANNNNTTC 2 cut(s) 215, 303
PfeI GAWTC 6 cut(s) 647, 656, 1017, 1205, 1292, 1559
PkrI GCNGC 4 cut(s) 172, 240, 493, 963
PleI GAGTC 2 cut(s) 647, 877
PpsI GAGTC 2 cut(s) 647, 877
Ppu21I YACGTR 1 cut(s) 1552
PsiI TTATAA 1 cut(s) 723
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspN4I GGNNCC 2 cut(s) 455, 777
PspPI GGNCC 3 cut(s) 454, 1165, 1626
PstI CTGCAG 1 cut(s) 321
PstNI CAGNNNCTG 1 cut(s) 524
PsuI RGATCY 2 cut(s) 775, 1457
PvuII CAGCTG 1 cut(s) 494
RsaI GTAC 4 cut(s) 437, 1219, 1306, 1495
RsaNI GTAC 4 cut(s) 436, 1218, 1305, 1494
RseI CAYNNNNRTG 3 cut(s) 155, 821, 827
SaqAI TTAA 3 cut(s) 131, 369, 981
SatI GCNGC 4 cut(s) 171, 239, 492, 962
Sau96I GGNCC 3 cut(s) 454, 1165, 1626
ScaI AGTACT 1 cut(s) 1495
SchI GAGTC 2 cut(s) 648, 878
ScrFI CCNGG 4 cut(s) 136, 270, 953, 954
SduI GDGCHC 1 cut(s) 976
SfaNI GCATC 4 cut(s) 37, 136, 235, 1432
SfcI CTRYAG 1 cut(s) 317
SinI GGWCC 2 cut(s) 454, 1165
SmaI CCCGGG 1 cut(s) 954
SmiMI CAYNNNNRTG 3 cut(s) 155, 821, 827
SmlI CTYRAG 1 cut(s) 858
SmoI CTYRAG 1 cut(s) 858
SnaBI TACGTA 1 cut(s) 1552
SphI GCATGC 1 cut(s) 1183
SsiI CCGC 5 cut(s) 26, 66, 379, 457, 961
SspMI CTAG 2 cut(s) 75, 977
StyD4I CCNGG 4 cut(s) 134, 268, 951, 952
StyI CCWWGG 2 cut(s) 33, 449
TaaI ACNGT 3 cut(s) 260, 842, 1121
TaiI ACGT 2 cut(s) 198, 1554
TaqI TCGA 3 cut(s) 180, 478, 637
TatI WGTACW 3 cut(s) 1217, 1304, 1493
TauI GCSGC 1 cut(s) 964
TfiI GAWTC 6 cut(s) 647, 656, 1017, 1205, 1292, 1559
Tru1I TTAA 3 cut(s) 131, 369, 981
Tru9I TTAA 3 cut(s) 131, 369, 981
TscAI CASTG 1 cut(s) 1473
TseFI GTSAC 2 cut(s) 1195, 1282
TseI GCWGC 3 cut(s) 170, 238, 491
Tsp45I GTSAC 2 cut(s) 1195, 1282
TspDTI ATGAA 9 cut(s) 123, 426, 851, 890, 1007, 1269, 1452, 1581, 1611
TspGWI ACGGA 1 cut(s) 1518
TspMI CCCGGG 1 cut(s) 952
TspRI CASTG 1 cut(s) 1473
VpaK11BI GGWCC 2 cut(s) 454, 1165
XapI RAATTY 3 cut(s) 717, 1036, 1358
XceI RCATGY 3 cut(s) 1003, 1183, 1320
XcmI CCANNNNNNNNNTGG 1 cut(s) 358
XmaI CCCGGG 1 cut(s) 952
XmiI GTMKAC 1 cut(s) 1543
XmnI GAANNNNTTC 2 cut(s) 215, 303
XspI CTAG 2 cut(s) 75, 977
ZrmI AGTACT 1 cut(s) 1495
Zsp2I ATGCAT 2 cut(s) 630, 943
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.