MD03G1172900.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014699
Physical Location & Seq
Forward (+)
23583430 .. 23585631
2202 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1172900.v1.1.491

Sequence Viewer

Length: 1257 bp
ATGTCTTCTCCCTCAGAGAGTCCAACTATGGAGCCTGTTGAGCAAAGTCATCCGGATCACAAGGCTTCTGTTACTATTATTACTCAACAGGTGGCTTCTTACAGCCTTAGCCCTAACAAGCAATCACCTGCACAAGGTGGTCAAGGTCAATTCACTCCGACGAGCGAGACTGGTGTTTCTTTGACAACCAACGTGTCAGCCGCCGCTGGCGGCACAGATCATGAGCTTTCGGAAGATGAGGCAAACAGTTTGCATAACAACCTGATAAAGGATCAAGATGAATTTTTCAATTCCTTCCCACCTGGCTATAGGTTCAACCCATTTGATGGCGAACTTATTGTGCATTATCTCATGAAAAAGGTCTTGGATCAGCCCTTGCCGCCAAACCGGATCTTTGAAGTTAACCTATACCGCTACAATCCCGAAGTGCTTGCTGAAAAATATGAGAAATATGGGGACAAATGGTATTTTTTTACTCCAAGGGACCGGAAGTATCGCAATGGAAGTCGCCCAAAGAGGGCAGCTGGGGATGGATATTGGAAGGCGACCGGAGCTGACAAGGAAGTGAAGTCATCTGATGGAGCTGTTGTTGGATCGAGAAAGGCTCTGGTTTTCTATAGGGGCAAACCCCCAAAGGGTGACAAAACCAACTGGATCATGCATGAGTTTAAAGTCAAGGATTCTCCTGTTCGAAGCAGAAGGGGTGAGAATGACATGAGGTTGGATAATTGGGTACTCTGTAGAATTTATAAGAAGGATGGTCAAAATGGCAAAAGATGTGAAAGTCGAAATCATGCTGAAATACATTCTCCATTTCCGCATAGAATAAATGACGAAAGCATGGAGATTGAGATGAGTGGAGATATTGTTGATCCTTTACATGAGTATGATCCTGCACTGGAGTATGACCATAAATACTCATATAAAGCATATCCTCATATGACACCTCTGCCAAATATTTCCGGTGGGCTGCCAGTTAACATGATTAGCAATAATCGAATTCAGTCTGTTGAAGCAGCCTCTATGAATGCTGGGAGCTACTATGCAAATATGGGGTACTCGCAACCAATATGCGTTAGACCTCCATCGATGTCTTCTGAGAGTGTTCTTGGGCCTTACAGATATATAGATGAATCAACTATTCTTCAAGATGACATGATGTTCGGCATGAAGGGGTTCGAACATAGCCTTTACAGTACAAATAATTATGCAGGGCAATTCTCAGCTTTGGATCATGTTTTTCCTTATACGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

419

Amino Acids

47.31

Weight (kDa)

5.99

Isoelectric Point (pI)

49.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 100 - 224 1.5e-41 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 750
AarI CACCTGC 1 cut(s) 136
Acc36I ACCTGC 1 cut(s) 136
AccB7I CCANNNNNTGG 1 cut(s) 326
AccIII TCCGGA 1 cut(s) 52
AciI CCGC 6 cut(s) 201, 204, 210, 380, 412, 818
AclWI GGATC 9 cut(s) 63, 279, 375, 398, 601, 662, 866, 884, 1239
AcsI RAATTY 3 cut(s) 281, 744, 999
AdeI CACNNNGTG 1 cut(s) 137
AfaI GTAC 3 cut(s) 735, 1058, 1198
AfiI CCNNNNNNNGG 5 cut(s) 134, 268, 326, 517, 635
AflIII ACRYGT 1 cut(s) 192
AgsI TTSAA 5 cut(s) 289, 316, 398, 1013, 1148
AjnI CCWGG 1 cut(s) 301
AjuI GAANNNNNNNTTGG 2 cut(s) 347, 379
AluBI AGCT 6 cut(s) 226, 524, 554, 584, 1038, 1226
AluI AGCT 6 cut(s) 226, 524, 554, 584, 1038, 1226
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 9 cut(s) 63, 279, 375, 398, 601, 662, 866, 884, 1239
Aor13HI TCCGGA 1 cut(s) 52
AoxI GGCC 1 cut(s) 1112
ApeKI GCWGC 3 cut(s) 521, 970, 1016
ApoI RAATTY 3 cut(s) 281, 744, 999
ArsI GACNNNNNNTTYG 2 cut(s) 1145, 1177
Asp700I GAANNNNTTC 1 cut(s) 1175
AspS9I GGNCC 2 cut(s) 484, 1112
AsuHPI GGTGA 3 cut(s) 117, 650, 716
AsuII TTCGAA 2 cut(s) 691, 1179
AvaII GGWCC 1 cut(s) 484
BbsI GAAGAC 1 cut(s) 1086
BbvI GCAGC 3 cut(s) 533, 957, 1028
BccI CCATC 5 cut(s) 320, 524, 572, 752, 1093
BcgI CGANNNNNNTGC 2 cut(s) 413, 447
BciT130I CCWGG 1 cut(s) 303
BcoDI GTCTC 1 cut(s) 161
BfmI CTRYAG 3 cut(s) 307, 616, 739
BfuAI ACCTGC 1 cut(s) 136
BisI GCNGC 7 cut(s) 201, 204, 211, 380, 522, 971, 1017
BlsI GCNGC 7 cut(s) 202, 205, 212, 381, 523, 972, 1018
Bme1390I CCNGG 1 cut(s) 303
Bme18I GGWCC 1 cut(s) 484
BmgT120I GGNCC 2 cut(s) 484, 1112
BmiI GGNNCC 2 cut(s) 33, 485
BmrFI CCNGG 1 cut(s) 303
BpiI GAAGAC 1 cut(s) 1086
BplI GAGNNNNNCTC 2 cut(s) 589, 621
BpmI CTGGAG 1 cut(s) 920
Bpu10I CCTNAGC 1 cut(s) 107
Bpu14I TTCGAA 2 cut(s) 691, 1179
Bsa29I ATCGAT 1 cut(s) 1088
BsaJI CCNNGG 1 cut(s) 479
BsaWI WCCGGW 5 cut(s) 52, 387, 486, 548, 962
BsaXI ACNNNNNCTCC 4 cut(s) 573, 603, 852, 882
Bsc4I CCNNNNNNNGG 5 cut(s) 134, 268, 326, 517, 635
Bse1I ACTGG 4 cut(s) 175, 656, 903, 974
Bse3DI GCAATG 1 cut(s) 505
BseAI TCCGGA 1 cut(s) 52
BseBI CCWGG 1 cut(s) 303
BseCI ATCGAT 1 cut(s) 1088
BseDI CCNNGG 1 cut(s) 479
BseGI GGATG 3 cut(s) 49, 535, 763
BseLI CCNNNNNNNGG 5 cut(s) 134, 268, 326, 517, 635
BseMI GCAATG 1 cut(s) 505
BseMII CTCAG 3 cut(s) 27, 1089, 1236
BseNI ACTGG 4 cut(s) 175, 656, 903, 974
BseXI GCAGC 3 cut(s) 533, 957, 1028
BseYI CCCAGC 2 cut(s) 524, 1031
BsgI GTGCAG 2 cut(s) 114, 879
Bsh1285I CGRYCG 1 cut(s) 549
BshFI GGCC 1 cut(s) 1114
BshVI ATCGAT 1 cut(s) 1088
BsiEI CGRYCG 1 cut(s) 549
BsiSI CCGG 5 cut(s) 53, 388, 487, 549, 963
BslFI GGGAC 2 cut(s) 470, 497
BslI CCNNNNNNNGG 5 cut(s) 134, 268, 326, 517, 635
BsmAI GTCTC 1 cut(s) 161
BsmFI GGGAC 2 cut(s) 470, 497
BsmI GAATGC 1 cut(s) 1033
BsnI GGCC 1 cut(s) 1114
Bsp119I TTCGAA 2 cut(s) 691, 1179
Bsp13I TCCGGA 1 cut(s) 52
BspACI CCGC 6 cut(s) 201, 204, 210, 380, 412, 818
BspANI GGCC 1 cut(s) 1114
BspCNI CTCAG 3 cut(s) 26, 1090, 1235
BspDI ATCGAT 1 cut(s) 1088
BspEI TCCGGA 1 cut(s) 52
BspHI TCATGA 2 cut(s) 220, 351
BspLI GGNNCC 2 cut(s) 33, 485
BspMI ACCTGC 1 cut(s) 136
BspPI GGATC 9 cut(s) 63, 279, 375, 398, 601, 662, 866, 884, 1239
BspT104I TTCGAA 2 cut(s) 691, 1179
BsrDI GCAATG 1 cut(s) 505
BsrI ACTGG 4 cut(s) 175, 656, 903, 974
BssECI CCNNGG 1 cut(s) 479
BssT1I CCWWGG 1 cut(s) 479
Bst2UI CCWGG 1 cut(s) 303
Bst4CI ACNGT 2 cut(s) 248, 1196
BstBI TTCGAA 2 cut(s) 691, 1179
BstC8I GCNNGC 2 cut(s) 208, 432
BstDEI CTNAG 4 cut(s) 13, 107, 1098, 1222
BstENI CCTNNNNNAGG 2 cut(s) 132, 266
BstF5I GGATG 3 cut(s) 49, 535, 763
BstMAI GTCTC 1 cut(s) 161
BstMCI CGRYCG 1 cut(s) 549
BstMWI GCNNNNNNNGC 3 cut(s) 40, 379, 551
BstNI CCWGG 1 cut(s) 303
BstSCI CCNGG 1 cut(s) 301
BstSFI CTRYAG 3 cut(s) 307, 616, 739
BstV1I GCAGC 3 cut(s) 533, 957, 1028
BstV2I GAAGAC 1 cut(s) 1086
BstX2I RGATCY 1 cut(s) 390
BstYI RGATCY 1 cut(s) 390
Bsu15I ATCGAT 1 cut(s) 1088
BsuRI GGCC 1 cut(s) 1114
BsuTUI ATCGAT 1 cut(s) 1088
BtsCI GGATG 3 cut(s) 49, 535, 763
BtsIMutI CAGTG 1 cut(s) 896
BveI ACCTGC 1 cut(s) 136
Cac8I GCNNGC 2 cut(s) 208, 432
CciI TCATGA 2 cut(s) 220, 351
Cfr13I GGNCC 2 cut(s) 484, 1112
ClaI ATCGAT 1 cut(s) 1088
Csp6I GTAC 3 cut(s) 734, 1057, 1197
CviQI GTAC 3 cut(s) 734, 1057, 1197
DdeI CTNAG 4 cut(s) 13, 107, 1098, 1222
DraI TTTAAA 1 cut(s) 670
DraIII CACNNNGTG 1 cut(s) 137
Eco130I CCWWGG 1 cut(s) 479
Eco47I GGWCC 1 cut(s) 484
EcoNI CCTNNNNNAGG 2 cut(s) 132, 266
EcoRI GAATTC 1 cut(s) 999
EcoRII CCWGG 1 cut(s) 301
EcoT14I CCWWGG 1 cut(s) 479
EcoT22I ATGCAT 1 cut(s) 663
ErhI CCWWGG 1 cut(s) 479
FaqI GGGAC 2 cut(s) 470, 497
FauNDI CATATG 1 cut(s) 939
Fnu4HI GCNGC 7 cut(s) 201, 204, 211, 380, 522, 971, 1017
FokI GGATG 3 cut(s) 36, 542, 770
Fsp4HI GCNGC 7 cut(s) 201, 204, 211, 380, 522, 971, 1017
GluI GCNGC 7 cut(s) 201, 204, 211, 380, 522, 971, 1017
GsaI CCCAGC 2 cut(s) 528, 1035
GsuI CTGGAG 1 cut(s) 920
HaeIII GGCC 1 cut(s) 1114
HapII CCGG 5 cut(s) 53, 388, 487, 549, 963
HincII GTYRAC 2 cut(s) 403, 979
HindII GTYRAC 2 cut(s) 403, 979
HinfI GANTC 3 cut(s) 19, 680, 1133
HpaI GTTAAC 2 cut(s) 403, 979
HpaII CCGG 5 cut(s) 53, 388, 487, 549, 963
HphI GGTGA 3 cut(s) 117, 650, 716
Hpy166II GTNNAC 2 cut(s) 403, 979
Hpy188I TCNGA 5 cut(s) 16, 159, 232, 577, 1099
Hpy188III TCNNGA 7 cut(s) 53, 221, 275, 352, 422, 597, 1148
Hpy8I GTNNAC 2 cut(s) 403, 979
Hpy99I CGWCG 1 cut(s) 163
HpyAV CCTTC 5 cut(s) 304, 535, 693, 748, 1165
HpyCH4III ACNGT 2 cut(s) 248, 1196
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 7 cut(s) 131, 253, 343, 661, 896, 1046, 1211
HpyF10VI GCNNNNNNNGC 3 cut(s) 40, 379, 551
HpyF3I CTNAG 4 cut(s) 13, 107, 1098, 1222
HpySE526I ACGT 1 cut(s) 192
Kpn2I TCCGGA 1 cut(s) 52
KspAI GTTAAC 2 cut(s) 403, 979
LmnI GCTCC 4 cut(s) 31, 551, 581, 1035
Lsp1109I GCAGC 3 cut(s) 533, 957, 1028
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 2 cut(s) 70, 638
MboII GAAGA 3 cut(s) 245, 1086, 1136
MflI RGATCY 1 cut(s) 390
MluCI AATT 8 cut(s) 149, 281, 289, 727, 744, 999, 1204, 1217
MlyI GAGTC 1 cut(s) 28
MmeI TCCRAC 4 cut(s) 47, 182, 571, 702
MnlI CCTC 8 cut(s) 22, 232, 510, 711, 945, 957, 1030, 1092
Mph1103I ATGCAT 1 cut(s) 663
MroI TCCGGA 1 cut(s) 52
MroXI GAANNNNTTC 1 cut(s) 1175
MseI TTAA 3 cut(s) 402, 669, 978
MslI CAYNNNNRTG 1 cut(s) 885
MspA1I CMGCKG 2 cut(s) 206, 524
MspI CCGG 5 cut(s) 53, 388, 487, 549, 963
MspR9I CCNGG 1 cut(s) 303
Mva1269I GAATGC 1 cut(s) 1033
MvaI CCWGG 1 cut(s) 303
MwoI GCNNNNNNNGC 3 cut(s) 40, 379, 551
NdeI CATATG 1 cut(s) 939
NlaIV GGNNCC 2 cut(s) 33, 485
NmuCI GTSAC 1 cut(s) 638
NsiI ATGCAT 1 cut(s) 663
NspV TTCGAA 2 cut(s) 691, 1179
PagI TCATGA 2 cut(s) 220, 351
PaqCI CACCTGC 1 cut(s) 136
PctI GAATGC 1 cut(s) 1033
PdmI GAANNNNTTC 1 cut(s) 1175
PfeI GAWTC 2 cut(s) 680, 1133
PflMI CCANNNNNTGG 1 cut(s) 326
PkrI GCNGC 7 cut(s) 202, 205, 212, 381, 523, 972, 1018
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
PsiI TTATAA 1 cut(s) 750
Psp6I CCWGG 1 cut(s) 301
PspFI CCCAGC 2 cut(s) 524, 1031
PspGI CCWGG 1 cut(s) 301
PspN4I GGNNCC 2 cut(s) 33, 485
PspPI GGNCC 2 cut(s) 484, 1112
PsuI RGATCY 1 cut(s) 390
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 3 cut(s) 735, 1058, 1198
RsaNI GTAC 3 cut(s) 734, 1057, 1197
RseI CAYNNNNRTG 1 cut(s) 885
SaqAI TTAA 3 cut(s) 402, 669, 978
SatI GCNGC 7 cut(s) 201, 204, 211, 380, 522, 971, 1017
Sau96I GGNCC 2 cut(s) 484, 1112
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 1 cut(s) 303
SfcI CTRYAG 3 cut(s) 307, 616, 739
SfuI TTCGAA 2 cut(s) 691, 1179
SinI GGWCC 1 cut(s) 484
SmiMI CAYNNNNRTG 1 cut(s) 885
Sse9I AATT 8 cut(s) 149, 281, 289, 727, 744, 999, 1204, 1217
SsiI CCGC 6 cut(s) 201, 204, 210, 380, 412, 818
SspI AATATT 1 cut(s) 958
StyD4I CCNGG 1 cut(s) 301
StyI CCWWGG 1 cut(s) 479
TaaI ACNGT 2 cut(s) 248, 1196
TaiI ACGT 1 cut(s) 195
TaqI TCGA 6 cut(s) 596, 691, 787, 997, 1088, 1179
TasI AATT 8 cut(s) 149, 281, 289, 727, 744, 999, 1204, 1217
TatI WGTACW 1 cut(s) 1196
TauI GCSGC 4 cut(s) 203, 206, 213, 382
TfiI GAWTC 2 cut(s) 680, 1133
Tru1I TTAA 3 cut(s) 402, 669, 978
Tru9I TTAA 3 cut(s) 402, 669, 978
TscAI CASTG 1 cut(s) 903
TseFI GTSAC 1 cut(s) 638
TseI GCWGC 3 cut(s) 521, 970, 1016
Tsp45I GTSAC 1 cut(s) 638
TspDTI ATGAA 5 cut(s) 294, 368, 1040, 1146, 1184
TspRI CASTG 1 cut(s) 903
Van91I CCANNNNNTGG 1 cut(s) 326
VpaK11BI GGWCC 1 cut(s) 484
XagI CCTNNNNNAGG 2 cut(s) 132, 266
XapI RAATTY 3 cut(s) 281, 744, 999
XmnI GAANNNNTTC 1 cut(s) 1175
Zsp2I ATGCAT 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.