Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0009922

Physical Location & Seq

Genomic Coordinates Forward (+)

22503702 .. 22507062

Gene Structure 3361 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g29420.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1128 bp

ATGGAGGGAAAGCGGTCAAGAGGGAGCTCACAGCCCCGTCCCCGCGAGTTGCTTCGCAAAATCAAAGAATTCACTCGAGCTCTTGTGGAAGATCTCACCAACGGCCGCCCACCGCAGATTCTCATCGATCGCTTCCGAAATTACTGCTCCGATTCCTCCGCAAATTGCTATTGCAGCTCTGATTTGCCAAAAGGGAAGGAGGCGCTGACTCTTCGAAAAGAAAGCCATGTGCATAGAATAGATGTTCTGCTTAGGGTTCTTGTAATTGTTCAGCAACTTCTGCAAGAAAACAGACATGGGTCTAAAAGAGATATCTATTACATGCACCCTTCGGTATTTTCAGATCAATCAGTTGTAGACCGTGCAATCAATGACATATGCATCCTTCTGCAGTGTAGTCGCCACAATTTGAATGTGGTGTCTGTAGGAAAGGGGTTGGTAATGGGTTGGTTAAGGTTTGCAGAGGCTGGAAACATATCTGATTGCATAAATAGACCAAACACTGCCTACCCTGTTCCAGTTCATGTTGAAGAGGTCAAAGATATTGTTAGTGTTGCCCAGTACATAATTATTGTGGAGAAAGAATCAGTTTTTCAACGATTGGCAAATGACCAGTTTTGTAACACAAACCACTGCATTGTCATCACTGGACGTGGATATCCTGATATTTCCACAAGAAGGTTGAGTCCTTTTACAATTTTCAAATGGGAGTTTCTGGGGCTCCTTGTTGATACTTTAAATCTCCCTGTCTATTGCTTGGTAGATTGTGATCCCTACGGTTTTGACATCTTGTCCACATATCGGTTTGGTTCTCTGCAAATGGCCTATGATGCAAAATTTCTACGGGTCCCGGAGATAATTTGGCTCGGAGCCTTCCCTTTGGATGCAGAGAAGTACCATCTTCCACAACAGTGTCTCCTTCCTTTGACTGCAGAAGACAAGAGGAAAACTGAAGCCATGTTACAGAGATGCTACTTAGAAAGAGAAGTGCCACACTGGAGATTGGAGCTACAGTTGATGCTGAAAAGCGGAGTGAAATTTGAGCTAGAAGCATTATCTGCGCATGCACTTTCCTTTCTGTCGATGGAGTACATACCATCTAAAATTCAAGGTGGAATGTTCATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000706 GO:0000724 GO:0000725 GO:0000729 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003916 GO:0003918 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007131 GO:0008094 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0016889 GO:0016894 GO:0017111 GO:0022402 GO:0022414 GO:0022607 GO:0031974 GO:0031981 GO:0033554 GO:0034641 GO:0035825 GO:0042138 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045132 GO:0045143 GO:0046483 GO:0048285 GO:0050896 GO:0051026 GO:0051276 GO:0051321 GO:0051716 GO:0061505 GO:0061982 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko04113 map04113

Pfam Domains

Protein Families

TP6A_N

Protein Analysis

376

Amino Acids

43.07

Weight (kDa)

8.2

Isoelectric Point (pI)

45.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TP6A_N	PF04406	79 - 139	3e-21	Type IIB DNA topoisomerase
TOP6A-Spo11_Toprim	PF21180	188 - 369	1.8e-59	Topoisomerase 6 subunit A/Spo11, Toprim domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009922)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13170 AT3G13170 AT3G13170
fragaria_vesca	FvH4_3g29420
malus_domestica	MD03G1184400.v1.1 MD03G1184700.v1.1
prunus_persica	Prupe.4G241800_v2.0.a1 Prupe.4G241800_v2.0.a1
pyrus_communis	pycom03g13780
rosa_chinensis	RchiOBHm_Chr5g0053841
rosa_laevigata	RLG00000034906
rosa_multiflora	Rmu_sc0007356.1_g000010
rosa_roxburghii	Rroxscaffold_1G00026760
rosa_rugosa	Rorug05G0283600 Rorug05G0283700
rosa_samantha	Rh5AG353400 Rh5BG365000 Rh5CG390100 Rh5DG380000
rosa_wichuraiana	Rw0G002140 Rw0G009550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1062
AccI	GTMKAC	1 cut(s)	357
AccII	CGCG	1 cut(s)	45
AciI	CCGC	6 cut(s)	13, 43, 106, 113, 159, 1029
AclWI	GGATC	1 cut(s)	764
AcoI	YGGCCR	1 cut(s)	103
AcsI	RAATTY	4 cut(s)	68, 836, 1037, 1104
AcuI	CTGAAG	1 cut(s)	972
AfaI	GTAC	3 cut(s)	563, 896, 1091
AfiI	CCNNNNNNNGG	2 cut(s)	678, 801
AgsI	TTSAA	5 cut(s)	412, 530, 596, 703, 1109
AhdI	GACNNNNNGTC	1 cut(s)	790
AjiI	CACGTC	1 cut(s)	653
AleI	CACNNNNGTG	1 cut(s)	910
AluBI	AGCT	5 cut(s)	27, 80, 177, 1009, 1045
AluI	AGCT	5 cut(s)	27, 80, 177, 1009, 1045
Alw21I	GWGCWC	2 cut(s)	29, 82
Alw26I	GTCTC	1 cut(s)	920
AlwI	GGATC	1 cut(s)	764
AlwNI	CAGNNNCTG	1 cut(s)	467
Ama87I	CYCGRG	1 cut(s)	75
AoxI	GGCC	2 cut(s)	103, 822
ApeKI	GCWGC	1 cut(s)	174
ApoI	RAATTY	4 cut(s)	68, 836, 1037, 1104
AspLEI	GCGC	2 cut(s)	205, 1063
AspS9I	GGNCC	1 cut(s)	847
AsuC2I	CCSGG	1 cut(s)	851
AsuHPI	GGTGA	1 cut(s)	88
AsuII	TTCGAA	1 cut(s)	214
AvaI	CYCGRG	1 cut(s)	75
AvaII	GGWCC	1 cut(s)	847
BanII	GRGCYC	3 cut(s)	29, 82, 723
BarI	GAAGNNNNNNTAC	2 cut(s)	945, 977
BbsI	GAAGAC	1 cut(s)	942
Bbv12I	GWGCWC	2 cut(s)	29, 82
BbvI	GCAGC	1 cut(s)	186
BccI	CCATC	3 cut(s)	906, 1078, 1105
BceAI	ACGGC	1 cut(s)	118
BcgI	CGANNNNNNTGC	4 cut(s)	126, 160, 380, 414
BcnI	CCSGG	1 cut(s)	851
BcoDI	GTCTC	1 cut(s)	920
BfaI	CTAG	1 cut(s)	1046
BfmI	CTRYAG	4 cut(s)	389, 423, 930, 1010
BfoI	RGCGCY	1 cut(s)	206
BglII	AGATCT	1 cut(s)	91
BisI	GCNGC	2 cut(s)	106, 175
BlsI	GCNGC	2 cut(s)	107, 176
Bme1390I	CCNGG	1 cut(s)	851
Bme18I	GGWCC	1 cut(s)	847
BmeRI	GACNNNNNGTC	1 cut(s)	790
BmeT110I	CYCGRG	1 cut(s)	75
BmgBI	CACGTC	1 cut(s)	653
BmgT120I	GGNCC	1 cut(s)	847
BmiI	GGNNCC	4 cut(s)	722, 848, 849, 871
BmrFI	CCNGG	1 cut(s)	851
BmrI	ACTGGG	1 cut(s)	553
BmsI	GCATC	5 cut(s)	390, 820, 874, 959, 1008
BmuI	ACTGGG	1 cut(s)	553
BoxI	GACNNNNGTC	1 cut(s)	298
BpiI	GAAGAC	1 cut(s)	942
BpmI	CTGGAG	1 cut(s)	1018
Bpu10I	CCTNAGC	1 cut(s)	251
Bpu14I	TTCGAA	1 cut(s)	214
BpuMI	CCSGG	1 cut(s)	851
Bsa29I	ATCGAT	1 cut(s)	126
BsaBI	GATNNNNATC	2 cut(s)	122, 768
BsaXI	ACNNNNNCTCC	4 cut(s)	900, 930, 998, 1028
Bsc4I	CCNNNNNNNGG	2 cut(s)	678, 801
Bse1I	ACTGG	5 cut(s)	518, 559, 613, 652, 1001
Bse8I	GATNNNNATC	2 cut(s)	122, 768
BseCI	ATCGAT	1 cut(s)	126
BseGI	GGATG	2 cut(s)	381, 889
BseJI	GATNNNNATC	2 cut(s)	122, 768
BseLI	CCNNNNNNNGG	2 cut(s)	678, 801
BseNI	ACTGG	5 cut(s)	518, 559, 613, 652, 1001
BseX3I	CGGCCG	1 cut(s)	103
BseXI	GCAGC	1 cut(s)	186
Bsh1236I	CGCG	1 cut(s)	45
Bsh1285I	CGRYCG	2 cut(s)	106, 130
BshFI	GGCC	2 cut(s)	105, 824
BshVI	ATCGAT	1 cut(s)	126
BsiEI	CGRYCG	2 cut(s)	106, 130
BsiHKAI	GWGCWC	2 cut(s)	29, 82
BsiHKCI	CYCGRG	1 cut(s)	75
BsiSI	CCGG	1 cut(s)	851
BslFI	GGGAC	2 cut(s)	24, 833
BslI	CCNNNNNNNGG	2 cut(s)	678, 801
BsmAI	GTCTC	1 cut(s)	920
BsmFI	GGGAC	2 cut(s)	24, 833
BsnI	GGCC	2 cut(s)	105, 824
BsoBI	CYCGRG	1 cut(s)	75
Bsp119I	TTCGAA	1 cut(s)	214
Bsp1286I	GDGCHC	3 cut(s)	29, 82, 723
Bsp143I	GATC	4 cut(s)	91, 127, 343, 769
BspACI	CCGC	6 cut(s)	13, 43, 106, 113, 159, 1029
BspANI	GGCC	2 cut(s)	105, 824
BspDI	ATCGAT	1 cut(s)	126
BspFNI	CGCG	1 cut(s)	45
BspLI	GGNNCC	4 cut(s)	722, 848, 849, 871
BspMAI	CTGCAG	2 cut(s)	393, 934
BspPI	GGATC	1 cut(s)	764
BspT104I	TTCGAA	1 cut(s)	214
BsrI	ACTGG	5 cut(s)	518, 559, 613, 652, 1001
BssMI	GATC	4 cut(s)	91, 127, 343, 769
Bst4CI	ACNGT	4 cut(s)	362, 779, 912, 1014
Bst6I	CTCTTC	2 cut(s)	216, 525
BstAPI	GCANNNNNTGC	2 cut(s)	280, 1058
BstBI	TTCGAA	1 cut(s)	214
BstC8I	GCNNGC	1 cut(s)	1065
BstDEI	CTNAG	2 cut(s)	251, 976
BstF5I	GGATG	2 cut(s)	381, 889
BstFNI	CGCG	1 cut(s)	45
BstH2I	RGCGCY	1 cut(s)	206
BstHHI	GCGC	2 cut(s)	205, 1063
BstKTI	GATC	4 cut(s)	94, 130, 346, 772
BstMAI	GTCTC	1 cut(s)	920
BstMBI	GATC	4 cut(s)	91, 127, 343, 769
BstMCI	CGRYCG	2 cut(s)	106, 130
BstMWI	GCNNNNNNNGC	4 cut(s)	174, 280, 830, 1058
BstNSI	RCATGY	2 cut(s)	325, 1067
BstPAI	GACNNNNGTC	1 cut(s)	298
BstSCI	CCNGG	1 cut(s)	849
BstSFI	CTRYAG	4 cut(s)	389, 423, 930, 1010
BstUI	CGCG	1 cut(s)	45
BstV1I	GCAGC	1 cut(s)	186
BstV2I	GAAGAC	1 cut(s)	942
BstX2I	RGATCY	1 cut(s)	91
BstYI	RGATCY	1 cut(s)	91
BstZI	CGGCCG	1 cut(s)	103
Bsu15I	ATCGAT	1 cut(s)	126
BsuRI	GGCC	2 cut(s)	105, 824
BsuTUI	ATCGAT	1 cut(s)	126
BtrI	CACGTC	1 cut(s)	653
BtsCI	GGATG	2 cut(s)	381, 889
BtsI	GCAGTG	3 cut(s)	398, 501, 631
BtsIMutI	CAGTG	6 cut(s)	398, 501, 631, 645, 917, 994
Cac8I	GCNNGC	1 cut(s)	1065
CaiI	CAGNNNCTG	1 cut(s)	467
CfoI	GCGC	2 cut(s)	205, 1063
Cfr13I	GGNCC	1 cut(s)	847
ClaI	ATCGAT	1 cut(s)	126
Csp6I	GTAC	3 cut(s)	562, 895, 1090
CviAII	CATG	6 cut(s)	227, 296, 322, 524, 958, 1064
CviQI	GTAC	3 cut(s)	562, 895, 1090
DdeI	CTNAG	2 cut(s)	251, 976
DpnI	GATC	4 cut(s)	93, 129, 345, 771
DpnII	GATC	4 cut(s)	91, 127, 343, 769
DraI	TTTAAA	1 cut(s)	738
DriI	GACNNNNNGTC	1 cut(s)	790
EaeI	YGGCCR	1 cut(s)	103
EagI	CGGCCG	1 cut(s)	103
Eam1104I	CTCTTC	2 cut(s)	216, 525
Eam1105I	GACNNNNNGTC	1 cut(s)	790
EarI	CTCTTC	2 cut(s)	216, 525
Ecl136II	GAGCTC	2 cut(s)	27, 80
EclXI	CGGCCG	1 cut(s)	103
Eco24I	GRGCYC	3 cut(s)	29, 82, 723
Eco32I	GATATC	2 cut(s)	313, 659
Eco47I	GGWCC	1 cut(s)	847
Eco52I	CGGCCG	1 cut(s)	103
Eco53kI	GAGCTC	2 cut(s)	27, 80
Eco57I	CTGAAG	1 cut(s)	972
Eco88I	CYCGRG	1 cut(s)	75
EcoICRI	GAGCTC	2 cut(s)	27, 80
EcoO109I	RGGNCCY	1 cut(s)	847
EcoRI	GAATTC	1 cut(s)	68
EcoRV	GATATC	2 cut(s)	313, 659
EcoT22I	ATGCAT	1 cut(s)	383
EcoT38I	GRGCYC	3 cut(s)	29, 82, 723
FaeI	CATG	6 cut(s)	230, 299, 325, 527, 961, 1067
FaqI	GGGAC	2 cut(s)	24, 833
FatI	CATG	6 cut(s)	226, 295, 321, 523, 957, 1063
FauI	CCCGC	1 cut(s)	50
FauNDI	CATATG	1 cut(s)	377
FblI	GTMKAC	1 cut(s)	357
Fnu4HI	GCNGC	2 cut(s)	106, 175
FokI	GGATG	2 cut(s)	368, 896
FriOI	GRGCYC	3 cut(s)	29, 82, 723
Fsp4HI	GCNGC	2 cut(s)	106, 175
FspBI	CTAG	1 cut(s)	1046
FspI	TGCGCA	1 cut(s)	1062
GlaI	GCGC	2 cut(s)	204, 1062
GluI	GCNGC	2 cut(s)	106, 175
GsuI	CTGGAG	1 cut(s)	1018
HaeII	RGCGCY	1 cut(s)	206
HaeIII	GGCC	2 cut(s)	105, 824
HapII	CCGG	1 cut(s)	851
HhaI	GCGC	2 cut(s)	205, 1063
Hin1II	CATG	6 cut(s)	230, 299, 325, 527, 961, 1067
Hin6I	GCGC	2 cut(s)	203, 1061
HinP1I	GCGC	2 cut(s)	203, 1061
HinfI	GANTC	5 cut(s)	118, 152, 208, 584, 685
HpaII	CCGG	1 cut(s)	851
HphI	GGTGA	1 cut(s)	88
Hpy166II	GTNNAC	2 cut(s)	358, 795
Hpy188I	TCNGA	6 cut(s)	137, 151, 181, 343, 481, 869
Hpy188III	TCNNGA	2 cut(s)	18, 662
Hpy8I	GTNNAC	2 cut(s)	358, 795
HpyAV	CCTTC	6 cut(s)	190, 339, 395, 672, 883, 929
HpyCH4III	ACNGT	4 cut(s)	362, 779, 912, 1014
HpyCH4IV	ACGT	1 cut(s)	652
HpyF10VI	GCNNNNNNNGC	4 cut(s)	174, 280, 830, 1058
HpyF3I	CTNAG	2 cut(s)	251, 976
HpySE526I	ACGT	1 cut(s)	652
Hsp92II	CATG	6 cut(s)	230, 299, 325, 527, 961, 1067
HspAI	GCGC	2 cut(s)	203, 1061
KflI	GGGWCCC	1 cut(s)	847
Kzo9I	GATC	4 cut(s)	91, 127, 343, 769
LmnI	GCTCC	5 cut(s)	24, 152, 726, 869, 1006
Lsp1109I	GCAGC	1 cut(s)	186
LweI	GCATC	5 cut(s)	390, 820, 874, 959, 1008
MaeI	CTAG	1 cut(s)	1046
MaeII	ACGT	1 cut(s)	652
MaeIII	GTNAC	2 cut(s)	620, 960
MalI	GATC	4 cut(s)	93, 129, 345, 771
MboI	GATC	4 cut(s)	91, 127, 343, 769
MboII	GAAGA	5 cut(s)	101, 203, 542, 893, 947
MflI	RGATCY	1 cut(s)	91
MhlI	GDGCHC	3 cut(s)	29, 82, 723
MlyI	GAGTC	2 cut(s)	202, 694
MnlI	CCTC	6 cut(s)	14, 166, 193, 457, 526, 936
Mph1103I	ATGCAT	1 cut(s)	383
MseI	TTAA	2 cut(s)	452, 737
MslI	CAYNNNNRTG	1 cut(s)	910
MspI	CCGG	1 cut(s)	851
MspR9I	CCNGG	1 cut(s)	851
MvnI	CGCG	1 cut(s)	45
MwoI	GCNNNNNNNGC	4 cut(s)	174, 280, 830, 1058
NciI	CCSGG	1 cut(s)	851
NdeI	CATATG	1 cut(s)	377
NdeII	GATC	4 cut(s)	91, 127, 343, 769
NlaIII	CATG	6 cut(s)	230, 299, 325, 527, 961, 1067
NlaIV	GGNNCC	4 cut(s)	722, 848, 849, 871
NsbI	TGCGCA	1 cut(s)	1062
NsiI	ATGCAT	1 cut(s)	383
NspI	RCATGY	2 cut(s)	325, 1067
NspV	TTCGAA	1 cut(s)	214
OliI	CACNNNNGTG	1 cut(s)	910
PaeI	GCATGC	1 cut(s)	1067
PaeR7I	CTCGAG	1 cut(s)	75
PfeI	GAWTC	3 cut(s)	118, 152, 584
PfoI	TCCNGGA	1 cut(s)	849
PkrI	GCNGC	2 cut(s)	107, 176
Ple19I	CGATCG	1 cut(s)	130
PleI	GAGTC	2 cut(s)	202, 693
PpsI	GAGTC	2 cut(s)	202, 693
PpuMI	RGGWCCY	1 cut(s)	847
PshAI	GACNNNNGTC	1 cut(s)	298
Psp124BI	GAGCTC	2 cut(s)	29, 82
Psp5II	RGGWCCY	1 cut(s)	847
PspN4I	GGNNCC	4 cut(s)	722, 848, 849, 871
PspPI	GGNCC	1 cut(s)	847
PspPPI	RGGWCCY	1 cut(s)	847
PspXI	VCTCGAGB	1 cut(s)	75
PstI	CTGCAG	2 cut(s)	393, 934
PstNI	CAGNNNCTG	1 cut(s)	467
PsuI	RGATCY	1 cut(s)	91
PvuI	CGATCG	1 cut(s)	130
RsaI	GTAC	3 cut(s)	563, 896, 1091
RsaNI	GTAC	3 cut(s)	562, 895, 1090
RseI	CAYNNNNRTG	1 cut(s)	910
SacI	GAGCTC	2 cut(s)	29, 82
SaqAI	TTAA	2 cut(s)	452, 737
SatI	GCNGC	2 cut(s)	106, 175
Sau3AI	GATC	4 cut(s)	91, 127, 343, 769
Sau96I	GGNCC	1 cut(s)	847
SchI	GAGTC	2 cut(s)	202, 694
ScrFI	CCNGG	1 cut(s)	851
SduI	GDGCHC	3 cut(s)	29, 82, 723
SfaNI	GCATC	5 cut(s)	390, 820, 874, 959, 1008
SfcI	CTRYAG	4 cut(s)	389, 423, 930, 1010
Sfr274I	CTCGAG	1 cut(s)	75
SfuI	TTCGAA	1 cut(s)	214
SinI	GGWCC	1 cut(s)	847
SlaI	CTCGAG	1 cut(s)	75
SmiMI	CAYNNNNRTG	1 cut(s)	910
SmlI	CTYRAG	1 cut(s)	75
SmoI	CTYRAG	1 cut(s)	75
SphI	GCATGC	1 cut(s)	1067
SsiI	CCGC	6 cut(s)	13, 43, 106, 113, 159, 1029
SspMI	CTAG	1 cut(s)	1046
SstI	GAGCTC	2 cut(s)	29, 82
StyD4I	CCNGG	1 cut(s)	849
TaaI	ACNGT	4 cut(s)	362, 779, 912, 1014
TaiI	ACGT	1 cut(s)	655
TaqI	TCGA	4 cut(s)	76, 126, 214, 1082
TatI	WGTACW	2 cut(s)	561, 1089
TauI	GCSGC	1 cut(s)	108
TfiI	GAWTC	3 cut(s)	118, 152, 584
Tru1I	TTAA	2 cut(s)	452, 737
Tru9I	TTAA	2 cut(s)	452, 737
TscAI	CASTG	6 cut(s)	398, 508, 638, 652, 917, 1001
TseI	GCWGC	1 cut(s)	174
TspDTI	ATGAA	2 cut(s)	512, 1111
TspRI	CASTG	6 cut(s)	398, 508, 638, 652, 917, 1001
VpaK11BI	GGWCC	1 cut(s)	847
XapI	RAATTY	4 cut(s)	68, 836, 1037, 1104
XceI	RCATGY	2 cut(s)	325, 1067
XhoI	CTCGAG	1 cut(s)	75
XmiI	GTMKAC	1 cut(s)	357
XspI	CTAG	1 cut(s)	1046
Zsp2I	ATGCAT	1 cut(s)	383

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.