Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0009922

Physical Location & Seq

Genomic Coordinates Reverse (-)

33713482 .. 33720639

Gene Structure 7158 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00026760.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1518 bp

ATGCATAGTTTGCTCGAGTTACAACCTGGTAAACACCCCTCTACTGTTCTTGACGTTTGGTTTTGGGATATGGTTAATGCACTTAATGGTGAGAAGCTGGAGTACTTCTTGATGGCCCTTTGGGTGGTTTGGATGGAGCGAAATAATATAGTTTGGAAAGCATCAGTTTCCAACCCCACTAACATGCATGCATGGGCTAAGTCATTTCTTAATGAGTATAAGCAATTACATAAACGTCAAGGAGGGAAGGACAAGAGATCACTTATCAAGTGGACTTGTCCACCAAGAGGAAGGTTGAAAATTAATATAGATGGAAGTTTTTGCACAGAGACTGGTGGTGGAGGTGTGGGGGTTGTGGTCAGAAACTTTGAAGGAACGTGCATGGCTACTTTTGCGCGACCCTTCCACTTTGCACAGTCAGCCATCCATATGGAGGCCGAAGCCCTTAGGGCGGGGTTGTTAATTGCAATTCAACAGGATTGGAAGGAAATTATTGTTGAAAGTGACTGCGTGAACTTGGTGAGTGCATTACGTGCAACTGATGATGATTTTTCAAGCATTGGAAGGATCTTGGATGATTGTAGAAGCTTTTGTAGCTCTGATTTGCCGAAAGGGAAGGAGACGCTCACACTTCGGAAAGAAACCCATGTTCATAGATTAGATGTTCTGCTTAGGGTTCTTGTAATTGTTCAGCAACTTCTGCAAGAAAACAGACATGGGTCTAAGAGAGATATCTATTACATGCACCCTTCAATATTTTCAGATCAGTCAGTTGTAGACCGTGCAATCAATGACATATGCATCCTTCTGCAGTGTAGTCGCCACAATTTGAACGTGGTGTCTGTAGGAAAGGGGTTGGTAATGGGCTGGTTAAGGTTCGCAGAGGCTGGAAACATGTGTGATTGCATAAATAGACCAAACACTGCTTACCCTGTTCCAGTTCATGTTGAAGAGGTCAAAGATATTGTTAGTGTTGCCCAGTACATACTTGTTGTGGAGAAAGAATCAGTTTTTCAACGATTGGCAAATGACCAGTTTTGTAACACCAACCACTGCATTGTCATCACTGGACGTGGATATCCTGATATTTCCACAAGAAGGTTTTTGGGGCTCCTTGTTGATACTTTACATATCCCTGTCTATTGCTTGGTAGATTGTGATCCCTACGGTTTTGACATCTTGTCCACATATCGGTTTGGTTCTCTGCAAATGGCATATGATGCAAAATTTCTACGGGTCCCCGAGATAATCTGGCTTGGAGCCTTCCCTTTGGACTCAGAGAAGTACTATCTTCCACACCAGTGTCTCCTTCCTTTGACTGCAGAAGACAAGAGGAAAACTGAAGCCATGTTACAGAGATGCTACTTAGAAAGAGAAGTGCCACAGTGGAGATTGGAGCTACAGTTGATGCTGGAAAGAGGCGTGAAATTTGAGCTAGAAGCATTGTCTGTGCATGAACTTTCCTTTTTGTCGACCGAGTACATACCATCAAAAATTCAAGGTGGATTGCTCATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000706 GO:0000724 GO:0000725 GO:0000729 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003916 GO:0003918 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007131 GO:0008094 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0016889 GO:0016894 GO:0017111 GO:0022402 GO:0022414 GO:0022607 GO:0031974 GO:0031981 GO:0033554 GO:0034641 GO:0035825 GO:0042138 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045132 GO:0045143 GO:0046483 GO:0048285 GO:0050896 GO:0051026 GO:0051276 GO:0051321 GO:0051716 GO:0061505 GO:0061982 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko04113 map04113

Pfam Domains

Protein Families

TP6A_N

Protein Analysis

505

Amino Acids

57.73

Weight (kDa)

6.47

Isoelectric Point (pI)

47.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RVT_3	PF13456	102 - 199	3e-14	Reverse transcriptase-like
TP6A_N	PF04406	219 - 279	4.7e-21	Type IIB DNA topoisomerase
TOP6A-Spo11_Toprim	PF21180	328 - 499	6.3e-64	Topoisomerase 6 subunit A/Spo11, Toprim domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009922)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13170 AT3G13170 AT3G13170
fragaria_vesca	FvH4_3g29420
malus_domestica	MD03G1184400.v1.1 MD03G1184700.v1.1
prunus_persica	Prupe.4G241800_v2.0.a1 Prupe.4G241800_v2.0.a1
pyrus_communis	pycom03g13780
rosa_chinensis	RchiOBHm_Chr5g0053841
rosa_laevigata	RLG00000034906
rosa_multiflora	Rmu_sc0007356.1_g000010
rosa_roxburghii	Rroxscaffold_1G00026760
rosa_rugosa	Rorug05G0283600 Rorug05G0283700
rosa_samantha	Rh5AG353400 Rh5BG365000 Rh5CG390100 Rh5DG380000
rosa_wichuraiana	Rw0G002140 Rw0G009550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	777, 1472
AccII	CGCG	1 cut(s)	397
AciI	CCGC	1 cut(s)	452
AclWI	GGATC	2 cut(s)	575, 1154
AcsI	RAATTY	3 cut(s)	1226, 1427, 1494
AcuI	CTGAAG	1 cut(s)	1362
AfaI	GTAC	4 cut(s)	104, 983, 1286, 1481
AfiI	CCNNNNNNNGG	6 cut(s)	124, 287, 433, 451, 1098, 1191
AflIII	ACRYGT	1 cut(s)	894
AhdI	GACNNNNNGTC	1 cut(s)	1180
AjiI	CACGTC	1 cut(s)	1073
AjnI	CCWGG	1 cut(s)	25
AleI	CACNNNNGTG	1 cut(s)	1300
AluBI	AGCT	5 cut(s)	97, 588, 597, 1399, 1435
AluI	AGCT	5 cut(s)	97, 588, 597, 1399, 1435
Alw26I	GTCTC	3 cut(s)	323, 614, 1310
AlwI	GGATC	2 cut(s)	575, 1154
AlwNI	CAGNNNCTG	2 cut(s)	332, 887
Ama87I	CYCGRG	2 cut(s)	14, 1241
AoxI	GGCC	2 cut(s)	114, 435
ApoI	RAATTY	3 cut(s)	1226, 1427, 1494
AseI	ATTAAT	1 cut(s)	303
AspLEI	GCGC	1 cut(s)	397
AspS9I	GGNCC	2 cut(s)	115, 1237
AsuHPI	GGTGA	2 cut(s)	101, 532
AvaI	CYCGRG	2 cut(s)	14, 1241
AvaII	GGWCC	1 cut(s)	1237
AxyI	CCTNAGG	1 cut(s)	446
BanII	GRGCYC	1 cut(s)	1113
BarI	GAAGNNNNNNTAC	4 cut(s)	86, 118, 1335, 1367
BbsI	GAAGAC	1 cut(s)	1332
BccI	CCATC	5 cut(s)	106, 127, 305, 431, 1495
BcgI	CGANNNNNNTGC	2 cut(s)	800, 834
BciT130I	CCWGG	1 cut(s)	27
BcoDI	GTCTC	3 cut(s)	323, 614, 1310
BfaI	CTAG	1 cut(s)	1436
BfmI	CTRYAG	4 cut(s)	809, 843, 1320, 1400
BglI	GCCNNNNNGGC	1 cut(s)	449
BmcAI	AGTACT	2 cut(s)	104, 1286
Bme1390I	CCNGG	1 cut(s)	27
Bme18I	GGWCC	1 cut(s)	1237
BmeRI	GACNNNNNGTC	1 cut(s)	1180
BmeT110I	CYCGRG	2 cut(s)	14, 1241
BmgBI	CACGTC	1 cut(s)	1073
BmgT120I	GGNCC	2 cut(s)	115, 1237
BmiI	GGNNCC	4 cut(s)	1112, 1238, 1239, 1261
BmrFI	CCNGG	1 cut(s)	27
BmrI	ACTGGG	1 cut(s)	973
BmsI	GCATC	5 cut(s)	170, 810, 1210, 1349, 1398
BmuI	ACTGGG	1 cut(s)	973
BoxI	GACNNNNGTC	1 cut(s)	718
BpiI	GAAGAC	1 cut(s)	1332
BpmI	CTGGAG	1 cut(s)	119
Bpu10I	CCTNAGC	1 cut(s)	671
BsaAI	YACGTR	1 cut(s)	533
BsaBI	GATNNNNATC	1 cut(s)	1158
BsaXI	ACNNNNNCTCC	2 cut(s)	1388, 1418
Bsc4I	CCNNNNNNNGG	6 cut(s)	124, 287, 433, 451, 1098, 1191
Bse1I	ACTGG	6 cut(s)	337, 938, 979, 1033, 1072, 1300
Bse21I	CCTNAGG	1 cut(s)	446
Bse8I	GATNNNNATC	1 cut(s)	1158
BseBI	CCWGG	1 cut(s)	27
BseGI	GGATG	4 cut(s)	138, 423, 580, 801
BseJI	GATNNNNATC	1 cut(s)	1158
BseLI	CCNNNNNNNGG	6 cut(s)	124, 287, 433, 451, 1098, 1191
BseMII	CTCAG	1 cut(s)	1290
BseNI	ACTGG	6 cut(s)	337, 938, 979, 1033, 1072, 1300
Bsh1236I	CGCG	1 cut(s)	397
Bsh1285I	CGRYCG	1 cut(s)	1476
BshFI	GGCC	2 cut(s)	116, 437
BsiEI	CGRYCG	1 cut(s)	1476
BsiHKCI	CYCGRG	2 cut(s)	14, 1241
BslFI	GGGAC	1 cut(s)	1223
BslI	CCNNNNNNNGG	6 cut(s)	124, 287, 433, 451, 1098, 1191
BsmAI	GTCTC	3 cut(s)	323, 614, 1310
BsmBI	CGTCTC	1 cut(s)	614
BsmFI	GGGAC	1 cut(s)	1223
BsnI	GGCC	2 cut(s)	116, 437
BsoBI	CYCGRG	2 cut(s)	14, 1241
Bsp1286I	GDGCHC	1 cut(s)	1113
Bsp143I	GATC	4 cut(s)	257, 567, 763, 1159
BspACI	CCGC	1 cut(s)	452
BspANI	GGCC	2 cut(s)	116, 437
BspCNI	CTCAG	1 cut(s)	1289
BspFNI	CGCG	1 cut(s)	397
BspLI	GGNNCC	4 cut(s)	1112, 1238, 1239, 1261
BspMAI	CTGCAG	2 cut(s)	813, 1324
BspPI	GGATC	2 cut(s)	575, 1154
BsrI	ACTGG	6 cut(s)	337, 938, 979, 1033, 1072, 1300
BssMI	GATC	4 cut(s)	257, 567, 763, 1159
Bst2UI	CCWGG	1 cut(s)	27
Bst4CI	ACNGT	6 cut(s)	46, 417, 782, 1169, 1386, 1404
Bst6I	CTCTTC	1 cut(s)	945
BstAPI	GCANNNNNTGC	4 cut(s)	10, 533, 700, 1220
BstBAI	YACGTR	1 cut(s)	533
BstC8I	GCNNGC	1 cut(s)	189
BstDEI	CTNAG	6 cut(s)	198, 446, 671, 723, 1276, 1366
BstF5I	GGATG	4 cut(s)	138, 423, 580, 801
BstFNI	CGCG	1 cut(s)	397
BstHHI	GCGC	1 cut(s)	397
BstKTI	GATC	4 cut(s)	260, 570, 766, 1162
BstMAI	GTCTC	3 cut(s)	323, 614, 1310
BstMBI	GATC	4 cut(s)	257, 567, 763, 1159
BstMCI	CGRYCG	1 cut(s)	1476
BstMWI	GCNNNNNNNGC	8 cut(s)	10, 392, 419, 449, 533, 594, 700, 1220
BstNI	CCWGG	1 cut(s)	27
BstNSI	RCATGY	4 cut(s)	187, 191, 745, 898
BstPAI	GACNNNNGTC	1 cut(s)	718
BstSCI	CCNGG	1 cut(s)	25
BstSFI	CTRYAG	4 cut(s)	809, 843, 1320, 1400
BstUI	CGCG	1 cut(s)	397
BstV2I	GAAGAC	1 cut(s)	1332
BstX2I	RGATCY	1 cut(s)	567
BstXI	CCANNNNNNTGG	1 cut(s)	430
BstYI	RGATCY	1 cut(s)	567
Bsu36I	CCTNAGG	1 cut(s)	446
BsuRI	GGCC	2 cut(s)	116, 437
BtrI	CACGTC	1 cut(s)	1073
BtsCI	GGATG	4 cut(s)	138, 423, 580, 801
BtsI	GCAGTG	3 cut(s)	818, 921, 1051
BtsIMutI	CAGTG	6 cut(s)	818, 921, 1051, 1065, 1307, 1391
Cac8I	GCNNGC	1 cut(s)	189
CaiI	CAGNNNCTG	2 cut(s)	332, 887
CfoI	GCGC	1 cut(s)	397
Cfr13I	GGNCC	2 cut(s)	115, 1237
CseI	GACGC	1 cut(s)	631
CsiI	ACCWGGT	1 cut(s)	25
Csp6I	GTAC	4 cut(s)	103, 982, 1285, 1480
CviQI	GTAC	4 cut(s)	103, 982, 1285, 1480
DdeI	CTNAG	6 cut(s)	198, 446, 671, 723, 1276, 1366
DpnI	GATC	4 cut(s)	259, 569, 765, 1161
DpnII	GATC	4 cut(s)	257, 567, 763, 1159
DriI	GACNNNNNGTC	1 cut(s)	1180
Eam1104I	CTCTTC	1 cut(s)	945
Eam1105I	GACNNNNNGTC	1 cut(s)	1180
EarI	CTCTTC	1 cut(s)	945
Eco24I	GRGCYC	1 cut(s)	1113
Eco32I	GATATC	2 cut(s)	733, 1079
Eco47I	GGWCC	1 cut(s)	1237
Eco57I	CTGAAG	1 cut(s)	1362
Eco81I	CCTNAGG	1 cut(s)	446
Eco88I	CYCGRG	2 cut(s)	14, 1241
EcoO109I	RGGNCCY	1 cut(s)	1237
EcoRII	CCWGG	1 cut(s)	25
EcoRV	GATATC	2 cut(s)	733, 1079
EcoT22I	ATGCAT	4 cut(s)	6, 189, 193, 803
EcoT38I	GRGCYC	1 cut(s)	1113
Esp3I	CGTCTC	1 cut(s)	614
FaqI	GGGAC	1 cut(s)	1223
FauI	CCCGC	1 cut(s)	445
FauNDI	CATATG	3 cut(s)	429, 797, 1216
FblI	GTMKAC	2 cut(s)	777, 1472
FokI	GGATG	4 cut(s)	145, 410, 587, 788
FriOI	GRGCYC	1 cut(s)	1113
FspBI	CTAG	1 cut(s)	1436
GlaI	GCGC	1 cut(s)	396
GsuI	CTGGAG	1 cut(s)	119
HaeIII	GGCC	2 cut(s)	116, 437
HgaI	GACGC	1 cut(s)	631
HhaI	GCGC	1 cut(s)	397
Hin6I	GCGC	1 cut(s)	395
HinP1I	GCGC	1 cut(s)	395
HincII	GTYRAC	1 cut(s)	1473
HindII	GTYRAC	1 cut(s)	1473
HindIII	AAGCTT	1 cut(s)	586
HinfI	GANTC	2 cut(s)	1004, 1274
HphI	GGTGA	2 cut(s)	101, 532
Hpy166II	GTNNAC	7 cut(s)	32, 273, 281, 514, 778, 1185, 1473
Hpy188I	TCNGA	5 cut(s)	362, 601, 636, 763, 1279
Hpy188III	TCNNGA	3 cut(s)	50, 109, 1082
Hpy8I	GTNNAC	7 cut(s)	32, 273, 281, 514, 778, 1185, 1473
HpyCH4III	ACNGT	6 cut(s)	46, 417, 782, 1169, 1386, 1404
HpyCH4IV	ACGT	6 cut(s)	54, 235, 377, 532, 834, 1072
HpyF10VI	GCNNNNNNNGC	8 cut(s)	10, 392, 419, 449, 533, 594, 700, 1220
HpyF3I	CTNAG	6 cut(s)	198, 446, 671, 723, 1276, 1366
HpySE526I	ACGT	6 cut(s)	54, 235, 377, 532, 834, 1072
HspAI	GCGC	1 cut(s)	395
KflI	GGGWCCC	1 cut(s)	1237
Kzo9I	GATC	4 cut(s)	257, 567, 763, 1159
LmnI	GCTCC	4 cut(s)	136, 1116, 1259, 1396
LweI	GCATC	5 cut(s)	170, 810, 1210, 1349, 1398
MabI	ACCWGGT	1 cut(s)	25
MaeI	CTAG	1 cut(s)	1436
MaeII	ACGT	6 cut(s)	54, 235, 377, 532, 834, 1072
MaeIII	GTNAC	4 cut(s)	18, 503, 1040, 1350
MalI	GATC	4 cut(s)	259, 569, 765, 1161
MboI	GATC	4 cut(s)	257, 567, 763, 1159
MboII	GAAGA	3 cut(s)	962, 1283, 1337
MflI	RGATCY	1 cut(s)	567
MhlI	GDGCHC	1 cut(s)	1113
MlyI	GAGTC	1 cut(s)	1268
MmeI	TCCRAC	1 cut(s)	195
MnlI	CCTC	9 cut(s)	49, 236, 281, 335, 427, 877, 946, 1326, 1412
Mph1103I	ATGCAT	4 cut(s)	6, 189, 193, 803
MseI	TTAA	6 cut(s)	75, 84, 210, 303, 461, 872
MslI	CAYNNNNRTG	3 cut(s)	182, 428, 1300
MspR9I	CCNGG	1 cut(s)	27
MvaI	CCWGG	1 cut(s)	27
MvnI	CGCG	1 cut(s)	397
MwoI	GCNNNNNNNGC	8 cut(s)	10, 392, 419, 449, 533, 594, 700, 1220
NdeI	CATATG	3 cut(s)	429, 797, 1216
NdeII	GATC	4 cut(s)	257, 567, 763, 1159
NlaIV	GGNNCC	4 cut(s)	1112, 1238, 1239, 1261
NmuCI	GTSAC	1 cut(s)	503
NsiI	ATGCAT	4 cut(s)	6, 189, 193, 803
NspI	RCATGY	4 cut(s)	187, 191, 745, 898
OliI	CACNNNNGTG	1 cut(s)	1300
PaeI	GCATGC	1 cut(s)	191
PaeR7I	CTCGAG	1 cut(s)	14
PciI	ACATGT	1 cut(s)	894
PfeI	GAWTC	1 cut(s)	1004
PleI	GAGTC	1 cut(s)	1268
PpsI	GAGTC	1 cut(s)	1268
Ppu21I	YACGTR	1 cut(s)	533
PpuMI	RGGWCCY	1 cut(s)	1237
PscI	ACATGT	1 cut(s)	894
PshAI	GACNNNNGTC	1 cut(s)	718
PshBI	ATTAAT	1 cut(s)	303
Psp5II	RGGWCCY	1 cut(s)	1237
Psp6I	CCWGG	1 cut(s)	25
PspGI	CCWGG	1 cut(s)	25
PspN4I	GGNNCC	4 cut(s)	1112, 1238, 1239, 1261
PspPI	GGNCC	2 cut(s)	115, 1237
PspPPI	RGGWCCY	1 cut(s)	1237
PspXI	VCTCGAGB	1 cut(s)	14
PstI	CTGCAG	2 cut(s)	813, 1324
PstNI	CAGNNNCTG	2 cut(s)	332, 887
PsuI	RGATCY	1 cut(s)	567
RsaI	GTAC	4 cut(s)	104, 983, 1286, 1481
RsaNI	GTAC	4 cut(s)	103, 982, 1285, 1480
RseI	CAYNNNNRTG	3 cut(s)	182, 428, 1300
SalI	GTCGAC	1 cut(s)	1471
SaqAI	TTAA	6 cut(s)	75, 84, 210, 303, 461, 872
Sau3AI	GATC	4 cut(s)	257, 567, 763, 1159
Sau96I	GGNCC	2 cut(s)	115, 1237
ScaI	AGTACT	2 cut(s)	104, 1286
SchI	GAGTC	1 cut(s)	1268
ScrFI	CCNGG	1 cut(s)	27
SduI	GDGCHC	1 cut(s)	1113
SexAI	ACCWGGT	1 cut(s)	25
SfaNI	GCATC	5 cut(s)	170, 810, 1210, 1349, 1398
SfcI	CTRYAG	4 cut(s)	809, 843, 1320, 1400
Sfr274I	CTCGAG	1 cut(s)	14
SinI	GGWCC	1 cut(s)	1237
SlaI	CTCGAG	1 cut(s)	14
SmiMI	CAYNNNNRTG	3 cut(s)	182, 428, 1300
SmlI	CTYRAG	1 cut(s)	14
SmoI	CTYRAG	1 cut(s)	14
SphI	GCATGC	1 cut(s)	191
SsiI	CCGC	1 cut(s)	452
SspI	AATATT	1 cut(s)	756
SspMI	CTAG	1 cut(s)	1436
StyD4I	CCNGG	1 cut(s)	25
TaaI	ACNGT	6 cut(s)	46, 417, 782, 1169, 1386, 1404
TaiI	ACGT	6 cut(s)	57, 238, 380, 535, 837, 1075
TaqI	TCGA	2 cut(s)	15, 1472
TaqII	GACCGA	1 cut(s)	1490
TatI	WGTACW	4 cut(s)	102, 981, 1284, 1479
TfiI	GAWTC	1 cut(s)	1004
Tru1I	TTAA	6 cut(s)	75, 84, 210, 303, 461, 872
Tru9I	TTAA	6 cut(s)	75, 84, 210, 303, 461, 872
TscAI	CASTG	6 cut(s)	818, 928, 1058, 1072, 1307, 1391
TseFI	GTSAC	1 cut(s)	503
Tsp45I	GTSAC	1 cut(s)	503
TspDTI	ATGAA	3 cut(s)	641, 932, 1470
TspRI	CASTG	6 cut(s)	818, 928, 1058, 1072, 1307, 1391
VpaK11BI	GGWCC	1 cut(s)	1237
VspI	ATTAAT	1 cut(s)	303
XapI	RAATTY	3 cut(s)	1226, 1427, 1494
XceI	RCATGY	4 cut(s)	187, 191, 745, 898
XhoI	CTCGAG	1 cut(s)	14
XmiI	GTMKAC	2 cut(s)	777, 1472
XspI	CTAG	1 cut(s)	1436
ZrmI	AGTACT	2 cut(s)	104, 1286
Zsp2I	ATGCAT	4 cut(s)	6, 189, 193, 803

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.