FvH4_3g36780

nucleotidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007641
Physical Location & Seq
Reverse (-)
31504076 .. 31506763
2688 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g36780.t5

Sequence Viewer

Length: 828 bp
ATGGAAAACAAGGATCAGTACCAACAATCCCCGGAACCAAAATATGAGTGCCTTCTATTTGATCTGGATGACACCCTCTATCCCCTGAGTTCCGGCATTTCAGTACAAATCACTAAGAATATTCAAGAATATATGCGTCATAAGCTTGGCATGGAGGAAAGTACAGTTCCTGAATTGTGTCTTTCGTTATACAAGGATTATGGGACGACAATGGCTGGTCTTAAGGCTCAAGGCTATAACTTTGAGTATGATGACTTCCACAGTTTTGTTCATGGGAGGTTGCCCTACAATTTGCTCAAGCCTGACCATGTTCTGAGGGGTCTTTTACTTAGTCTGCCTGTTCGGAAAGTAATCTTTACAAATTCGGATAAGAATCATACCATTACGGTTCTCAAAAGGCTTGGAATAGAGGACTGTTTCGAGTCAATTATATGCTTTGAGACTCTGAATCCCACCAACAGTGCTGATGGTTCTGCTGACGCAGAAGAAACTGACTTTGTGCAACAACCAAATACCGATGCTGTAACTCCAAGGAGCCCAGTTATCTGCAAACCATTTGAAAATGCCTATGTAGAAGCTTTTAAGATAGCAAACATTAACCCTCAAAGAACGTTATTCTTTGATGATAGCATTCGTAATTTGGAAACTGCAAAACAAGTGGGTCTGCAAACAGTATGGGTTGGCACCTCTCACCGAACCAAAGATGTGGATCATGCACTGGAGAGCATCCACAATATGAGGGAGGCATTGCCAGAACTCTGGGCTGAGCAGTCTGAGAATGTTATATATTCAAGAGAGGTTGCAATTGAGACACCAGTAGAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_like

Protein Analysis

276

Amino Acids

31.33

Weight (kDa)

4.84

Isoelectric Point (pI)

46.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 16 - 221 2.8e-08 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 683
AclI AACGTT 1 cut(s) 611
AclWI GGATC 2 cut(s) 21, 717
AcsI RAATTY 1 cut(s) 361
AfaI GTAC 3 cut(s) 20, 105, 163
AflII CTTAAG 1 cut(s) 221
AgsI TTSAA 3 cut(s) 125, 560, 792
AluBI AGCT 3 cut(s) 145, 578, 824
AluI AGCT 3 cut(s) 145, 578, 824
Alw26I GTCTC 2 cut(s) 434, 803
AlwI GGATC 2 cut(s) 21, 717
AlwNI CAGNNNCTG 1 cut(s) 170
ApoI RAATTY 1 cut(s) 361
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 1 cut(s) 683
BanI GGYRCC 1 cut(s) 683
BanII GRGCYC 1 cut(s) 539
BarI GAAGNNNNNNTAC 2 cut(s) 239, 271
BccI CCATC 1 cut(s) 461
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 2 cut(s) 434, 803
BfrI CTTAAG 1 cut(s) 221
BlpI GCTNAGC 1 cut(s) 765
Bme1390I CCNGG 1 cut(s) 32
BmiI GGNNCC 3 cut(s) 36, 536, 685
BmrFI CCNGG 1 cut(s) 32
BmrI ACTGGG 1 cut(s) 533
BmsI GCATC 2 cut(s) 508, 735
BmuI ACTGGG 1 cut(s) 533
BpmI CTGGAG 1 cut(s) 740
Bpu1102I GCTNAGC 1 cut(s) 765
BpuEI CTTGAG 2 cut(s) 213, 281
BpuMI CCSGG 1 cut(s) 32
BsaBI GATNNNNATC 2 cut(s) 372, 708
BsaJI CCNNGG 2 cut(s) 30, 530
Bse1I ACTGG 3 cut(s) 539, 723, 815
Bse3DI GCAATG 1 cut(s) 746
Bse8I GATNNNNATC 2 cut(s) 372, 708
BseDI CCNNGG 2 cut(s) 30, 530
BseGI GGATG 2 cut(s) 73, 726
BseJI GATNNNNATC 2 cut(s) 372, 708
BseMI GCAATG 1 cut(s) 746
BseMII CTCAG 4 cut(s) 77, 305, 756, 765
BseNI ACTGG 3 cut(s) 539, 723, 815
BshNI GGYRCC 1 cut(s) 683
BsiSI CCGG 2 cut(s) 32, 93
BslFI GGGAC 1 cut(s) 217
BsmAI GTCTC 2 cut(s) 434, 803
BsmFI GGGAC 1 cut(s) 217
BsmI GAATGC 1 cut(s) 630
Bsp1286I GDGCHC 1 cut(s) 539
Bsp143I GATC 3 cut(s) 13, 61, 709
Bsp1720I GCTNAGC 1 cut(s) 765
BspCNI CTCAG 4 cut(s) 78, 306, 757, 766
BspLI GGNNCC 3 cut(s) 36, 536, 685
BspPI GGATC 2 cut(s) 21, 717
BspT107I GGYRCC 1 cut(s) 683
BspTI CTTAAG 1 cut(s) 221
BsrDI GCAATG 1 cut(s) 746
BsrI ACTGG 3 cut(s) 539, 723, 815
BssECI CCNNGG 2 cut(s) 30, 530
BssMI GATC 3 cut(s) 13, 61, 709
BssT1I CCWWGG 1 cut(s) 530
Bst4CI ACNGT 6 cut(s) 166, 263, 388, 416, 461, 673
BstAFI CTTAAG 1 cut(s) 221
BstDEI CTNAG 6 cut(s) 86, 114, 314, 329, 765, 774
BstF5I GGATG 2 cut(s) 73, 726
BstKTI GATC 3 cut(s) 16, 64, 712
BstMAI GTCTC 2 cut(s) 434, 803
BstMBI GATC 3 cut(s) 13, 61, 709
BstMWI GCNNNNNNNGC 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 30
BstXI CCANNNNNNTGG 2 cut(s) 706, 759
BtsCI GGATG 2 cut(s) 73, 726
BtsIMutI CAGTG 2 cut(s) 466, 716
CaiI CAGNNNCTG 1 cut(s) 170
CseI GACGC 2 cut(s) 125, 488
Csp6I GTAC 3 cut(s) 19, 104, 162
CspCI CAANNNNNGTGG 2 cut(s) 639, 674
CviAII CATG 4 cut(s) 151, 272, 308, 713
CviQI GTAC 3 cut(s) 19, 104, 162
DdeI CTNAG 6 cut(s) 86, 114, 314, 329, 765, 774
DpnI GATC 3 cut(s) 15, 63, 711
DpnII GATC 3 cut(s) 13, 61, 709
Eco130I CCWWGG 1 cut(s) 530
Eco24I GRGCYC 1 cut(s) 539
EcoT14I CCWWGG 1 cut(s) 530
EcoT38I GRGCYC 1 cut(s) 539
ErhI CCWWGG 1 cut(s) 530
FaeI CATG 4 cut(s) 154, 275, 311, 716
FaqI GGGAC 1 cut(s) 217
FatI CATG 4 cut(s) 150, 271, 307, 712
FokI GGATG 2 cut(s) 80, 713
FriOI GRGCYC 1 cut(s) 539
GsuI CTGGAG 1 cut(s) 740
HapII CCGG 2 cut(s) 32, 93
HgaI GACGC 2 cut(s) 125, 488
Hin1II CATG 4 cut(s) 154, 275, 311, 716
HindIII AAGCTT 3 cut(s) 143, 576, 822
HinfI GANTC 4 cut(s) 373, 422, 442, 448
HpaII CCGG 2 cut(s) 32, 93
HphI GGTGA 1 cut(s) 683
Hpy188I TCNGA 5 cut(s) 315, 345, 367, 447, 775
Hpy188III TCNNGA 4 cut(s) 65, 125, 170, 792
HpyAV CCTTC 1 cut(s) 62
HpyCH4III ACNGT 6 cut(s) 166, 263, 388, 416, 461, 673
HpyCH4IV ACGT 1 cut(s) 611
HpyCH4V TGCA 6 cut(s) 502, 549, 650, 667, 716, 803
HpyF10VI GCNNNNNNNGC 1 cut(s) 142
HpyF3I CTNAG 6 cut(s) 86, 114, 314, 329, 765, 774
HpySE526I ACGT 1 cut(s) 611
Hsp92II CATG 4 cut(s) 154, 275, 311, 716
Kzo9I GATC 3 cut(s) 13, 61, 709
LmnI GCTCC 1 cut(s) 534
LweI GCATC 2 cut(s) 508, 735
MaeII ACGT 1 cut(s) 611
MaeIII GTNAC 1 cut(s) 523
MalI GATC 3 cut(s) 15, 63, 711
MboI GATC 3 cut(s) 13, 61, 709
MboII GAAGA 1 cut(s) 497
MfeI CAATTG 1 cut(s) 804
MhlI GDGCHC 1 cut(s) 539
MluCI AATT 6 cut(s) 173, 289, 361, 426, 637, 804
MlyI GAGTC 2 cut(s) 431, 436
MseI TTAA 4 cut(s) 222, 582, 597, 826
MspCI CTTAAG 1 cut(s) 221
MspI CCGG 2 cut(s) 32, 93
MspR9I CCNGG 1 cut(s) 32
MunI CAATTG 1 cut(s) 804
Mva1269I GAATGC 1 cut(s) 630
MwoI GCNNNNNNNGC 1 cut(s) 142
NciI CCSGG 1 cut(s) 32
NdeII GATC 3 cut(s) 13, 61, 709
NlaIII CATG 4 cut(s) 154, 275, 311, 716
NlaIV GGNNCC 3 cut(s) 36, 536, 685
PctI GAATGC 1 cut(s) 630
PfeI GAWTC 2 cut(s) 373, 448
PleI GAGTC 2 cut(s) 430, 436
PpsI GAGTC 2 cut(s) 430, 436
Psp1406I AACGTT 1 cut(s) 611
PspN4I GGNNCC 3 cut(s) 36, 536, 685
PstNI CAGNNNCTG 1 cut(s) 170
RsaI GTAC 3 cut(s) 20, 105, 163
RsaNI GTAC 3 cut(s) 19, 104, 162
SaqAI TTAA 4 cut(s) 222, 582, 597, 826
Sau3AI GATC 3 cut(s) 13, 61, 709
SchI GAGTC 2 cut(s) 431, 436
ScrFI CCNGG 1 cut(s) 32
SduI GDGCHC 1 cut(s) 539
SetI ASST 7 cut(s) 147, 281, 580, 614, 689, 801, 826
SfaNI GCATC 2 cut(s) 508, 735
SmlI CTYRAG 3 cut(s) 221, 228, 296
SmoI CTYRAG 3 cut(s) 221, 228, 296
Sse9I AATT 6 cut(s) 173, 289, 361, 426, 637, 804
SspI AATATT 1 cut(s) 121
StyD4I CCNGG 1 cut(s) 30
StyI CCWWGG 1 cut(s) 530
TaaI ACNGT 6 cut(s) 166, 263, 388, 416, 461, 673
TaiI ACGT 1 cut(s) 614
TaqI TCGA 1 cut(s) 420
TasI AATT 6 cut(s) 173, 289, 361, 426, 637, 804
TatI WGTACW 2 cut(s) 103, 161
TfiI GAWTC 2 cut(s) 373, 448
Tru1I TTAA 4 cut(s) 222, 582, 597, 826
Tru9I TTAA 4 cut(s) 222, 582, 597, 826
TscAI CASTG 2 cut(s) 466, 723
TspDTI ATGAA 1 cut(s) 260
TspRI CASTG 2 cut(s) 466, 723
Vha464I CTTAAG 1 cut(s) 221
XapI RAATTY 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.