RchiOBHm_Chr5g0065831

nucleotidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007641
Physical Location & Seq
Reverse (-)
71776773 .. 71778524
1752 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34167

Sequence Viewer

Length: 828 bp
ATGGAAAATAAGGATCAATACCAACAATCCCCGGAACCAAAATACGAGTGCCTTCTATTTGATATAGATGACACCCTCTATCCCCTGAGTTCCGGCATTTCAGTACAAATCACCAAGAATATTCAAGAATATATGCTTCATAAGCTTGGGATGGAAGAAAGTACAGTTCCTGAATTGTGTCTTTCGTTGTACAAGGATTATGGGACGACAATGGCCGGTCTTAAGGCTCAAGGCTATAACTTTGAGTATGATGACTTCCACAGTTTTGTTCATGGGAGGTTGCCCTACGATTTGCTTAGGCCTGACCATGTTCTGAGGGGTCTTTTACTTAGTCTGCCTGTTCGGAAAGTAATCTTTACGAATTCAGATAAGAATCATGCCCCTACAGTGCTCAAAAGGCTTGGAATAGAGGACTGTTTTGAGTCAATCATATGCTTTGAGACTCTGAATCCCACCAACAGTGCTGATGATTCTGCTGGTGCAGAAGAAACTGAATTTGTGCAACAACCAAATACCGATGCTGTAATTCCAAGGAGCCCAGTTGTCTGCAAACCATTTGAAAATGCCTTTGAAGAAGCTTTTAAGATAGCAAACATCAACCCTCAAAGAACGTTATTCTTTGACGATAGCATTCGTAATTTGCAAACTGCAAAACAAGTGGGTCTGCAAACAGTATGGGTTGGCGCCTCCCACCGAACCAAAGATGTGGATCATGCACTGGAGAGCATCCACAATATGAGGGAGGCATTGCCAGAACTCTGGGCCGAGCAGTCTGAGAATGTTAGATATTCGAGAGAGGTTGCAATTGAGACACCAGTAGAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_like

Protein Analysis

275

Amino Acids

31.31

Weight (kDa)

4.8

Isoelectric Point (pI)

54.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 16 - 221 7.6e-09 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 683
AclI AACGTT 1 cut(s) 611
AclWI GGATC 2 cut(s) 21, 717
AcoI YGGCCR 1 cut(s) 213
AcsI RAATTY 2 cut(s) 361, 494
AcyI GRCGYC 1 cut(s) 684
AfaI GTAC 3 cut(s) 105, 163, 191
AflII CTTAAG 1 cut(s) 221
AgsI TTSAA 3 cut(s) 125, 560, 572
AluBI AGCT 3 cut(s) 145, 578, 824
AluI AGCT 3 cut(s) 145, 578, 824
Alw21I GWGCWC 1 cut(s) 393
Alw26I GTCTC 2 cut(s) 434, 803
AlwI GGATC 2 cut(s) 21, 717
AlwNI CAGNNNCTG 1 cut(s) 170
AoxI GGCC 3 cut(s) 213, 299, 762
ApoI RAATTY 2 cut(s) 361, 494
AspLEI GCGC 1 cut(s) 686
AspS9I GGNCC 1 cut(s) 762
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 1 cut(s) 103
BanI GGYRCC 1 cut(s) 683
BanII GRGCYC 1 cut(s) 539
BarI GAAGNNNNNNTAC 2 cut(s) 239, 271
Bbv12I GWGCWC 1 cut(s) 393
BccI CCATC 1 cut(s) 145
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 2 cut(s) 434, 803
BfmI CTRYAG 1 cut(s) 384
BfoI RGCGCY 1 cut(s) 687
BfrI CTTAAG 1 cut(s) 221
Bme1390I CCNGG 1 cut(s) 32
BmgT120I GGNCC 1 cut(s) 762
BmiI GGNNCC 3 cut(s) 36, 536, 685
BmrFI CCNGG 1 cut(s) 32
BmrI ACTGGG 1 cut(s) 533
BmsI GCATC 2 cut(s) 508, 735
BmuI ACTGGG 1 cut(s) 533
BpmI CTGGAG 1 cut(s) 740
Bpu10I CCTNAGC 1 cut(s) 296
BpuEI CTTGAG 1 cut(s) 213
BpuMI CCSGG 1 cut(s) 32
BsaBI GATNNNNATC 2 cut(s) 372, 708
BsaHI GRCGYC 1 cut(s) 684
BsaJI CCNNGG 2 cut(s) 30, 530
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 3 cut(s) 539, 723, 815
Bse3DI GCAATG 1 cut(s) 746
Bse8I GATNNNNATC 2 cut(s) 372, 708
BseDI CCNNGG 2 cut(s) 30, 530
BseGI GGATG 2 cut(s) 156, 726
BseJI GATNNNNATC 2 cut(s) 372, 708
BseMI GCAATG 1 cut(s) 746
BseMII CTCAG 3 cut(s) 77, 305, 765
BseNI ACTGG 3 cut(s) 539, 723, 815
BsgI GTGCAG 1 cut(s) 501
BshFI GGCC 3 cut(s) 215, 301, 764
BshNI GGYRCC 1 cut(s) 683
BsiHKAI GWGCWC 1 cut(s) 393
BsiSI CCGG 3 cut(s) 32, 93, 216
BslFI GGGAC 1 cut(s) 217
BsmAI GTCTC 2 cut(s) 434, 803
BsmFI GGGAC 1 cut(s) 217
BsmI GAATGC 1 cut(s) 630
BsnI GGCC 3 cut(s) 215, 301, 764
Bsp1286I GDGCHC 2 cut(s) 393, 539
Bsp1407I TGTACA 1 cut(s) 189
Bsp143I GATC 2 cut(s) 13, 709
BspANI GGCC 3 cut(s) 215, 301, 764
BspCNI CTCAG 3 cut(s) 78, 306, 766
BspLI GGNNCC 3 cut(s) 36, 536, 685
BspPI GGATC 2 cut(s) 21, 717
BspT107I GGYRCC 1 cut(s) 683
BspTI CTTAAG 1 cut(s) 221
BsrDI GCAATG 1 cut(s) 746
BsrFI RCCGGY 1 cut(s) 215
BsrGI TGTACA 1 cut(s) 189
BsrI ACTGG 3 cut(s) 539, 723, 815
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 2 cut(s) 30, 530
BssMI GATC 2 cut(s) 13, 709
BssNI GRCGYC 1 cut(s) 684
BssT1I CCWWGG 1 cut(s) 530
Bst4CI ACNGT 6 cut(s) 166, 263, 388, 416, 461, 673
BstACI GRCGYC 1 cut(s) 684
BstAFI CTTAAG 1 cut(s) 221
BstAUI TGTACA 1 cut(s) 189
BstDEI CTNAG 6 cut(s) 86, 296, 314, 329, 774, 825
BstF5I GGATG 2 cut(s) 156, 726
BstH2I RGCGCY 1 cut(s) 687
BstHHI GCGC 1 cut(s) 686
BstKTI GATC 2 cut(s) 16, 712
BstMAI GTCTC 2 cut(s) 434, 803
BstMBI GATC 2 cut(s) 13, 709
BstMWI GCNNNNNNNGC 2 cut(s) 142, 397
BstSCI CCNGG 1 cut(s) 30
BstSFI CTRYAG 1 cut(s) 384
BstXI CCANNNNNNTGG 2 cut(s) 706, 759
BsuRI GGCC 3 cut(s) 215, 301, 764
BtsCI GGATG 2 cut(s) 156, 726
BtsIMutI CAGTG 3 cut(s) 393, 466, 716
CaiI CAGNNNCTG 1 cut(s) 170
CfoI GCGC 1 cut(s) 686
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 762
Csp6I GTAC 3 cut(s) 104, 162, 190
CspCI CAANNNNNGTGG 2 cut(s) 639, 674
CviAII CATG 4 cut(s) 272, 308, 377, 713
CviQI GTAC 3 cut(s) 104, 162, 190
DdeI CTNAG 6 cut(s) 86, 296, 314, 329, 774, 825
DinI GGCGCC 1 cut(s) 685
DpnI GATC 2 cut(s) 15, 711
DpnII GATC 2 cut(s) 13, 709
EaeI YGGCCR 1 cut(s) 213
Eco130I CCWWGG 1 cut(s) 530
Eco147I AGGCCT 1 cut(s) 301
Eco24I GRGCYC 1 cut(s) 539
EcoRI GAATTC 1 cut(s) 361
EcoT14I CCWWGG 1 cut(s) 530
EcoT38I GRGCYC 1 cut(s) 539
EgeI GGCGCC 1 cut(s) 685
EheI GGCGCC 1 cut(s) 685
ErhI CCWWGG 1 cut(s) 530
FaeI CATG 4 cut(s) 275, 311, 380, 716
FaqI GGGAC 1 cut(s) 217
FatI CATG 4 cut(s) 271, 307, 376, 712
FauNDI CATATG 1 cut(s) 431
FokI GGATG 2 cut(s) 163, 713
FriOI GRGCYC 1 cut(s) 539
GlaI GCGC 1 cut(s) 685
GsuI CTGGAG 1 cut(s) 740
HaeII RGCGCY 1 cut(s) 687
HaeIII GGCC 3 cut(s) 215, 301, 764
HapII CCGG 3 cut(s) 32, 93, 216
HhaI GCGC 1 cut(s) 686
Hin1I GRCGYC 1 cut(s) 684
Hin1II CATG 4 cut(s) 275, 311, 380, 716
Hin6I GCGC 1 cut(s) 684
HinP1I GCGC 1 cut(s) 684
HindIII AAGCTT 3 cut(s) 143, 576, 822
HinfI GANTC 5 cut(s) 373, 422, 442, 448, 470
HpaII CCGG 3 cut(s) 32, 93, 216
HphI GGTGA 1 cut(s) 103
Hpy188I TCNGA 5 cut(s) 315, 345, 367, 447, 775
Hpy188III TCNNGA 3 cut(s) 125, 170, 792
HpyAV CCTTC 1 cut(s) 62
HpyCH4III ACNGT 6 cut(s) 166, 263, 388, 416, 461, 673
HpyCH4IV ACGT 1 cut(s) 611
HpyCH4V TGCA 8 cut(s) 482, 502, 549, 643, 650, 667, 716, 803
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 397
HpyF3I CTNAG 6 cut(s) 86, 296, 314, 329, 774, 825
HpySE526I ACGT 1 cut(s) 611
Hsp92I GRCGYC 1 cut(s) 684
Hsp92II CATG 4 cut(s) 275, 311, 380, 716
HspAI GCGC 1 cut(s) 684
KasI GGCGCC 1 cut(s) 683
Kzo9I GATC 2 cut(s) 13, 709
LmnI GCTCC 1 cut(s) 534
LweI GCATC 2 cut(s) 508, 735
MaeII ACGT 1 cut(s) 611
MalI GATC 2 cut(s) 15, 711
MboI GATC 2 cut(s) 13, 709
MboII GAAGA 3 cut(s) 167, 497, 584
MfeI CAATTG 1 cut(s) 804
MhlI GDGCHC 2 cut(s) 393, 539
MluCI AATT 6 cut(s) 173, 361, 494, 525, 637, 804
Mly113I GGCGCC 1 cut(s) 684
MlyI GAGTC 2 cut(s) 431, 436
MnlI CCTC 9 cut(s) 86, 270, 309, 403, 612, 697, 732, 736, 790
MseI TTAA 2 cut(s) 222, 582
MspCI CTTAAG 1 cut(s) 221
MspI CCGG 3 cut(s) 32, 93, 216
MspR9I CCNGG 1 cut(s) 32
MunI CAATTG 1 cut(s) 804
Mva1269I GAATGC 1 cut(s) 630
MwoI GCNNNNNNNGC 2 cut(s) 142, 397
NarI GGCGCC 1 cut(s) 684
NciI CCSGG 1 cut(s) 32
NdeI CATATG 1 cut(s) 431
NdeII GATC 2 cut(s) 13, 709
NlaIII CATG 4 cut(s) 275, 311, 380, 716
NlaIV GGNNCC 3 cut(s) 36, 536, 685
NmeAIII GCCGAG 1 cut(s) 790
PceI AGGCCT 1 cut(s) 301
PctI GAATGC 1 cut(s) 630
PfeI GAWTC 3 cut(s) 373, 448, 470
PleI GAGTC 2 cut(s) 430, 436
PluTI GGCGCC 1 cut(s) 687
PpsI GAGTC 2 cut(s) 430, 436
Psp1406I AACGTT 1 cut(s) 611
PspN4I GGNNCC 3 cut(s) 36, 536, 685
PspPI GGNCC 1 cut(s) 762
PstNI CAGNNNCTG 1 cut(s) 170
RsaI GTAC 3 cut(s) 105, 163, 191
RsaNI GTAC 3 cut(s) 104, 162, 190
SaqAI TTAA 2 cut(s) 222, 582
Sau3AI GATC 2 cut(s) 13, 709
Sau96I GGNCC 1 cut(s) 762
SchI GAGTC 2 cut(s) 431, 436
ScrFI CCNGG 1 cut(s) 32
SduI GDGCHC 2 cut(s) 393, 539
SetI ASST 6 cut(s) 147, 281, 580, 614, 801, 826
SfaNI GCATC 2 cut(s) 508, 735
SfcI CTRYAG 1 cut(s) 384
SfoI GGCGCC 1 cut(s) 685
SmlI CTYRAG 2 cut(s) 221, 228
SmoI CTYRAG 2 cut(s) 221, 228
Sse9I AATT 6 cut(s) 173, 361, 494, 525, 637, 804
SseBI AGGCCT 1 cut(s) 301
SspDI GGCGCC 1 cut(s) 683
SspI AATATT 1 cut(s) 121
StuI AGGCCT 1 cut(s) 301
StyD4I CCNGG 1 cut(s) 30
StyI CCWWGG 1 cut(s) 530
TaaI ACNGT 6 cut(s) 166, 263, 388, 416, 461, 673
TaiI ACGT 1 cut(s) 614
TaqI TCGA 1 cut(s) 791
TasI AATT 6 cut(s) 173, 361, 494, 525, 637, 804
TatI WGTACW 3 cut(s) 103, 161, 189
TfiI GAWTC 3 cut(s) 373, 448, 470
Tru1I TTAA 2 cut(s) 222, 582
Tru9I TTAA 2 cut(s) 222, 582
TscAI CASTG 3 cut(s) 393, 466, 723
TspDTI ATGAA 2 cut(s) 128, 260
TspRI CASTG 3 cut(s) 393, 466, 723
Vha464I CTTAAG 1 cut(s) 221
XapI RAATTY 2 cut(s) 361, 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.