MD11G1097600.v1.1

nucleotidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007641
Physical Location & Seq
Forward (+)
8095356 .. 8097882
2527 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1097600.v1.1.491

Sequence Viewer

Length: 822 bp
ATGGAAAATCAGGTTCAATATCACCAATATGAGTGCCTTCTATTTGATCTAGACGACACCCTTTACCCTCTGAGTTCTGGAATTTCAGTACAAATCACTAGGAATATTAAAGAATATATGATTCAAAGGCTTGGCATGGACGAAAGTGAAGTTCCCGAATTGTGTATTTCTTTGTACAAGGATTATGGGACAACAATGGCCGGTCTTAAGGCTCAGGGCTATAACTTCGATTATGATGACTTTCATAGTTTTGTTCATGGGAGGTTGCCCTACGAGTTGCTTACTCCTGATCCTGTCCTGAGGGGCCTTTTACTTAGTCTGCCTATCCGCAAAGTAATTTTCACGAATTCGGATAAGAAACATGCGGATACTGTGCTCAAAAGGCTTGGAATAGAGGACTGCTTTGAGTCAGTTATATGTTTTGAGACACTGAATCCCACCAACAATGCCGATGATTCTGTTGGTGCAGAAGAAACTGAACACATTGAACAACCAAATACTGGTGCGGTGGTTCCAAAGACCCCAGTTGTCTGCAAACCATTTGAGCTTGCATACGAAGAAGCCTTTAAATTTGCAAACATCGACCCTCAAAGAACATTATTCTTCGATGATAGCATTCGCAATATACAAACTGCAAAGCAGTGCGGCCTCCATACAGTATTGGTTGGCACCTCCCACCGCACCAAAGGAGCGGATTATGCACTTGAGAGCATCCATAATATGAAGGAAGCACTGCCAGAACTCTGGGAAATCACCACTATGCAGTCTGAAAATGTCCAAACTACAAGAGAGATTGCAATTGAGCAACTGGTGGAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_like

Protein Analysis

274

Amino Acids

31.02

Weight (kDa)

4.7

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 11 - 216 6.6e-10 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 668
AccB7I CCANNNNNTGG 1 cut(s) 500
AccBSI CCGCTC 1 cut(s) 692
AciI CCGC 6 cut(s) 328, 365, 506, 645, 679, 692
AclWI GGATC 1 cut(s) 284
AcoI YGGCCR 1 cut(s) 198
AcsI RAATTY 3 cut(s) 81, 346, 569
AfaI GTAC 2 cut(s) 90, 176
AfiI CCNNNNNNNGG 1 cut(s) 500
AflII CTTAAG 1 cut(s) 206
AgsI TTSAA 3 cut(s) 17, 125, 488
AluBI AGCT 2 cut(s) 547, 818
AluI AGCT 2 cut(s) 547, 818
Alw21I GWGCWC 1 cut(s) 378
Alw26I GTCTC 1 cut(s) 419
AlwI GGATC 1 cut(s) 284
AoxI GGCC 3 cut(s) 198, 304, 646
ApoI RAATTY 3 cut(s) 81, 346, 569
AspS9I GGNCC 1 cut(s) 304
AsuHPI GGTGA 2 cut(s) 14, 745
AxyI CCTNAGG 1 cut(s) 299
BanI GGYRCC 1 cut(s) 668
Bbv12I GWGCWC 1 cut(s) 378
BciVI GTATCC 1 cut(s) 361
BcoDI GTCTC 1 cut(s) 419
BfaI CTAG 2 cut(s) 50, 99
BfrI CTTAAG 1 cut(s) 206
BfuI GTATCC 1 cut(s) 361
BisI GCNGC 1 cut(s) 646
BlsI GCNGC 1 cut(s) 647
BmgT120I GGNCC 1 cut(s) 304
BmiI GGNNCC 3 cut(s) 305, 513, 670
BmrI ACTGGG 1 cut(s) 518
BmsI GCATC 1 cut(s) 720
BmuI ACTGGG 1 cut(s) 518
Bpu10I CCTNAGC 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 725
Bsc4I CCNNNNNNNGG 1 cut(s) 500
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 3 cut(s) 505, 524, 813
Bse21I CCTNAGG 1 cut(s) 299
BseGI GGATG 1 cut(s) 711
BseLI CCNNNNNNNGG 1 cut(s) 500
BseMII CTCAG 3 cut(s) 62, 227, 290
BseNI ACTGG 3 cut(s) 505, 524, 813
BsgI GTGCAG 1 cut(s) 486
BshFI GGCC 3 cut(s) 200, 306, 648
BshNI GGYRCC 1 cut(s) 668
BsiHKAI GWGCWC 1 cut(s) 378
BsiSI CCGG 1 cut(s) 201
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 1 cut(s) 500
BsmAI GTCTC 1 cut(s) 419
BsmFI GGGAC 1 cut(s) 202
BsmI GAATGC 1 cut(s) 615
BsnI GGCC 3 cut(s) 200, 306, 648
Bsp1286I GDGCHC 1 cut(s) 378
Bsp1407I TGTACA 1 cut(s) 174
Bsp143I GATC 2 cut(s) 46, 289
BspACI CCGC 6 cut(s) 328, 365, 506, 645, 679, 692
BspANI GGCC 3 cut(s) 200, 306, 648
BspCNI CTCAG 3 cut(s) 63, 226, 291
BspLI GGNNCC 3 cut(s) 305, 513, 670
BspPI GGATC 1 cut(s) 284
BspT107I GGYRCC 1 cut(s) 668
BspTI CTTAAG 1 cut(s) 206
BsrBI CCGCTC 1 cut(s) 692
BsrFI RCCGGY 1 cut(s) 200
BsrGI TGTACA 1 cut(s) 174
BsrI ACTGG 3 cut(s) 505, 524, 813
BssAI RCCGGY 1 cut(s) 200
BssMI GATC 2 cut(s) 46, 289
Bst4CI ACNGT 2 cut(s) 373, 658
BstAFI CTTAAG 1 cut(s) 206
BstAUI TGTACA 1 cut(s) 174
BstC8I GCNNGC 1 cut(s) 549
BstDEI CTNAG 5 cut(s) 71, 213, 299, 314, 819
BstF5I GGATG 1 cut(s) 711
BstKTI GATC 2 cut(s) 49, 292
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 2 cut(s) 46, 289
BstMWI GCNNNNNNNGC 2 cut(s) 382, 698
BstNSI RCATGY 1 cut(s) 365
BstXI CCANNNNNNTGG 1 cut(s) 744
Bsu36I CCTNAGG 1 cut(s) 299
BsuI GTATCC 1 cut(s) 361
BsuRI GGCC 3 cut(s) 200, 306, 648
BtsCI GGATG 1 cut(s) 711
BtsI GCAGTG 2 cut(s) 647, 731
BtsIMutI CAGTG 3 cut(s) 428, 647, 731
Cac8I GCNNGC 1 cut(s) 549
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 1 cut(s) 304
Csp6I GTAC 2 cut(s) 89, 175
CviAII CATG 3 cut(s) 136, 257, 362
CviQI GTAC 2 cut(s) 89, 175
DdeI CTNAG 5 cut(s) 71, 213, 299, 314, 819
DpnI GATC 2 cut(s) 48, 291
DpnII GATC 2 cut(s) 46, 289
DraI TTTAAA 1 cut(s) 568
EaeI YGGCCR 1 cut(s) 198
Eco81I CCTNAGG 1 cut(s) 299
EcoO109I RGGNCCY 1 cut(s) 304
EcoRI GAATTC 1 cut(s) 346
FaeI CATG 3 cut(s) 139, 260, 365
FaqI GGGAC 1 cut(s) 202
FatI CATG 3 cut(s) 135, 256, 361
Fnu4HI GCNGC 1 cut(s) 646
FokI GGATG 1 cut(s) 698
Fsp4HI GCNGC 1 cut(s) 646
FspBI CTAG 2 cut(s) 50, 99
GluI GCNGC 1 cut(s) 646
HaeIII GGCC 3 cut(s) 200, 306, 648
HapII CCGG 1 cut(s) 201
Hin1II CATG 3 cut(s) 139, 260, 365
HindIII AAGCTT 1 cut(s) 816
HinfI GANTC 4 cut(s) 121, 407, 433, 455
HpaII CCGG 1 cut(s) 201
HphI GGTGA 2 cut(s) 14, 745
Hpy188I TCNGA 3 cut(s) 72, 352, 769
Hpy188III TCNNGA 6 cut(s) 50, 78, 155, 287, 298, 343
HpyAV CCTTC 2 cut(s) 47, 718
HpyCH4III ACNGT 2 cut(s) 373, 658
HpyCH4V TGCA 8 cut(s) 467, 534, 551, 575, 635, 701, 763, 797
HpyF10VI GCNNNNNNNGC 2 cut(s) 382, 698
HpyF3I CTNAG 5 cut(s) 71, 213, 299, 314, 819
Hsp92II CATG 3 cut(s) 139, 260, 365
Kzo9I GATC 2 cut(s) 46, 289
LmnI GCTCC 1 cut(s) 689
LweI GCATC 1 cut(s) 720
MaeI CTAG 2 cut(s) 50, 99
MalI GATC 2 cut(s) 48, 291
MbiI CCGCTC 1 cut(s) 692
MboI GATC 2 cut(s) 46, 289
MboII GAAGA 3 cut(s) 482, 569, 595
MfeI CAATTG 1 cut(s) 798
MhlI GDGCHC 1 cut(s) 378
MluCI AATT 6 cut(s) 81, 158, 336, 346, 569, 798
MlyI GAGTC 1 cut(s) 416
MnlI CCTC 7 cut(s) 78, 255, 294, 388, 597, 659, 682
MseI TTAA 3 cut(s) 108, 207, 567
MslI CAYNNNNRTG 2 cut(s) 27, 758
MspCI CTTAAG 1 cut(s) 206
MspI CCGG 1 cut(s) 201
MunI CAATTG 1 cut(s) 798
Mva1269I GAATGC 1 cut(s) 615
MwoI GCNNNNNNNGC 2 cut(s) 382, 698
NdeII GATC 2 cut(s) 46, 289
NlaIII CATG 3 cut(s) 139, 260, 365
NlaIV GGNNCC 3 cut(s) 305, 513, 670
NspI RCATGY 1 cut(s) 365
PctI GAATGC 1 cut(s) 615
PfeI GAWTC 3 cut(s) 121, 433, 455
PflMI CCANNNNNTGG 1 cut(s) 500
PkrI GCNGC 1 cut(s) 647
PleI GAGTC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 415
PspN4I GGNNCC 3 cut(s) 305, 513, 670
PspPI GGNCC 1 cut(s) 304
RsaI GTAC 2 cut(s) 90, 176
RsaNI GTAC 2 cut(s) 89, 175
RseI CAYNNNNRTG 2 cut(s) 27, 758
SaqAI TTAA 3 cut(s) 108, 207, 567
SatI GCNGC 1 cut(s) 646
Sau3AI GATC 2 cut(s) 46, 289
Sau96I GGNCC 1 cut(s) 304
SchI GAGTC 1 cut(s) 416
SduI GDGCHC 1 cut(s) 378
SetI ASST 5 cut(s) 15, 266, 549, 674, 820
SfaNI GCATC 1 cut(s) 720
SmiMI CAYNNNNRTG 2 cut(s) 27, 758
SmlI CTYRAG 2 cut(s) 206, 704
SmoI CTYRAG 2 cut(s) 206, 704
Sse9I AATT 6 cut(s) 81, 158, 336, 346, 569, 798
SsiI CCGC 6 cut(s) 328, 365, 506, 645, 679, 692
SspI AATATT 1 cut(s) 106
SspMI CTAG 2 cut(s) 50, 99
TaaI ACNGT 2 cut(s) 373, 658
TaqI TCGA 3 cut(s) 228, 582, 606
TasI AATT 6 cut(s) 81, 158, 336, 346, 569, 798
TatI WGTACW 2 cut(s) 88, 174
TauI GCSGC 1 cut(s) 648
TfiI GAWTC 3 cut(s) 121, 433, 455
Tru1I TTAA 3 cut(s) 108, 207, 567
Tru9I TTAA 3 cut(s) 108, 207, 567
TscAI CASTG 3 cut(s) 435, 647, 738
TspDTI ATGAA 3 cut(s) 233, 245, 737
TspRI CASTG 3 cut(s) 435, 647, 738
Van91I CCANNNNNTGG 1 cut(s) 500
Vha464I CTTAAG 1 cut(s) 206
XapI RAATTY 3 cut(s) 81, 346, 569
XbaI TCTAGA 1 cut(s) 49
XceI RCATGY 1 cut(s) 365
XspI CTAG 2 cut(s) 50, 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.