Rh5CG471300

nucleotidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0007641
Physical Location & Seq
Reverse (-)
65672193 .. 65674847
2655 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG471300.1

Sequence Viewer

Length: 618 bp
ATGGCCGGTCTTAAGGCTCAAGGCTATAACTTTGAGTATGATGACTTCCACAGTTTTGTTCATGGGAGGTTGCCCTACGATTTGCTTAAGCCTGACCATGTTCTGAGGGGTCTTTTACTTAGTCTGCCTGTTCGGAAAGTAATCTTTACGAATTCAGATAAGAATCATGCCCCTACAGTGCTCAAAAGGCTTGGAATAGAGGACTGTTTTGAGTCAATCATATGCTTTGAGACTCTGAATCCCACCAACAGTGCTGATGATTCTGCTGGTGCAGAAGAAACTGAATTTGTGCAACAACCAAATACCGATGCTGTAATTCCAAGGAGCCCAGTTGTCTGCAAACCATTTGAAAATGCCTTTGAAGAAGCTTTTAAGATAGCAAACATCAACCCTCAAAGAACGTTATTCTTTGACGATAGCATTCGTAATTTGCAAACTGCAAAACAAGTGGGTCTGCAAACAGTATGGGTTGGCGCCTCCCACCGAACCAAAGATGTGGATCATGCACTGGAGAGCATCCACAATATGAGGGAGGCATTGCCAGAACTCTGGGCCGAGCAGTCTGAGAATGTTAGATATTCGAGAGAGGTTGCAATTGAGACACCAGTAGAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_like

Protein Analysis

205

Amino Acids

23.17

Weight (kDa)

5.06

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 45 - 151 1.7e-07 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 473
AclI AACGTT 1 cut(s) 401
AclWI GGATC 1 cut(s) 507
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 151, 284
AcyI GRCGYC 1 cut(s) 474
AflII CTTAAG 2 cut(s) 11, 86
AgsI TTSAA 2 cut(s) 350, 362
AluBI AGCT 2 cut(s) 368, 614
AluI AGCT 2 cut(s) 368, 614
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 2 cut(s) 224, 593
AlwI GGATC 1 cut(s) 507
AoxI GGCC 2 cut(s) 3, 552
ApoI RAATTY 2 cut(s) 151, 284
AspLEI GCGC 1 cut(s) 476
AspS9I GGNCC 1 cut(s) 552
BanI GGYRCC 1 cut(s) 473
BanII GRGCYC 1 cut(s) 329
BarI GAAGNNNNNNTAC 2 cut(s) 29, 61
Bbv12I GWGCWC 1 cut(s) 183
BcoDI GTCTC 2 cut(s) 224, 593
BfmI CTRYAG 1 cut(s) 174
BfoI RGCGCY 1 cut(s) 477
BfrI CTTAAG 2 cut(s) 11, 86
BmgT120I GGNCC 1 cut(s) 552
BmiI GGNNCC 2 cut(s) 326, 475
BmrI ACTGGG 1 cut(s) 323
BmsI GCATC 2 cut(s) 298, 525
BmuI ACTGGG 1 cut(s) 323
BpmI CTGGAG 1 cut(s) 530
BsaBI GATNNNNATC 2 cut(s) 162, 498
BsaHI GRCGYC 1 cut(s) 474
BsaJI CCNNGG 1 cut(s) 320
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 3 cut(s) 329, 513, 605
Bse3DI GCAATG 1 cut(s) 536
Bse8I GATNNNNATC 2 cut(s) 162, 498
BseDI CCNNGG 1 cut(s) 320
BseGI GGATG 1 cut(s) 516
BseJI GATNNNNATC 2 cut(s) 162, 498
BseMI GCAATG 1 cut(s) 536
BseMII CTCAG 2 cut(s) 95, 555
BseNI ACTGG 3 cut(s) 329, 513, 605
BsgI GTGCAG 1 cut(s) 291
BshFI GGCC 2 cut(s) 5, 554
BshNI GGYRCC 1 cut(s) 473
BsiHKAI GWGCWC 1 cut(s) 183
BsiSI CCGG 1 cut(s) 6
BsmAI GTCTC 2 cut(s) 224, 593
BsmI GAATGC 1 cut(s) 420
BsnI GGCC 2 cut(s) 5, 554
Bsp1286I GDGCHC 2 cut(s) 183, 329
Bsp143I GATC 1 cut(s) 499
BspANI GGCC 2 cut(s) 5, 554
BspCNI CTCAG 2 cut(s) 96, 556
BspLI GGNNCC 2 cut(s) 326, 475
BspPI GGATC 1 cut(s) 507
BspT107I GGYRCC 1 cut(s) 473
BspTI CTTAAG 2 cut(s) 11, 86
BsrDI GCAATG 1 cut(s) 536
BsrFI RCCGGY 1 cut(s) 5
BsrI ACTGG 3 cut(s) 329, 513, 605
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 1 cut(s) 320
BssMI GATC 1 cut(s) 499
BssNI GRCGYC 1 cut(s) 474
BssT1I CCWWGG 1 cut(s) 320
Bst4CI ACNGT 5 cut(s) 53, 178, 206, 251, 463
BstACI GRCGYC 1 cut(s) 474
BstAFI CTTAAG 2 cut(s) 11, 86
BstDEI CTNAG 4 cut(s) 104, 119, 564, 615
BstF5I GGATG 1 cut(s) 516
BstH2I RGCGCY 1 cut(s) 477
BstHHI GCGC 1 cut(s) 476
BstKTI GATC 1 cut(s) 502
BstMAI GTCTC 2 cut(s) 224, 593
BstMBI GATC 1 cut(s) 499
BstMWI GCNNNNNNNGC 1 cut(s) 187
BstSFI CTRYAG 1 cut(s) 174
BstXI CCANNNNNNTGG 2 cut(s) 496, 549
BsuRI GGCC 2 cut(s) 5, 554
BtsCI GGATG 1 cut(s) 516
BtsIMutI CAGTG 3 cut(s) 183, 256, 506
CfoI GCGC 1 cut(s) 476
Cfr10I RCCGGY 1 cut(s) 5
Cfr13I GGNCC 1 cut(s) 552
CspCI CAANNNNNGTGG 2 cut(s) 429, 464
CviAII CATG 4 cut(s) 62, 98, 167, 503
CviJI RGCY 9 cut(s) 5, 17, 24, 91, 190, 327, 368, 554, 614
CviKI_1 RGCY 9 cut(s) 5, 17, 24, 91, 190, 327, 368, 554, 614
DdeI CTNAG 4 cut(s) 104, 119, 564, 615
DinI GGCGCC 1 cut(s) 475
DpnI GATC 1 cut(s) 501
DpnII GATC 1 cut(s) 499
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 320
Eco24I GRGCYC 1 cut(s) 329
EcoRI GAATTC 1 cut(s) 151
EcoT14I CCWWGG 1 cut(s) 320
EcoT38I GRGCYC 1 cut(s) 329
EgeI GGCGCC 1 cut(s) 475
EheI GGCGCC 1 cut(s) 475
ErhI CCWWGG 1 cut(s) 320
FaeI CATG 4 cut(s) 65, 101, 170, 506
FatI CATG 4 cut(s) 61, 97, 166, 502
FauNDI CATATG 1 cut(s) 221
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 1 cut(s) 329
GlaI GCGC 1 cut(s) 475
GsuI CTGGAG 1 cut(s) 530
HaeII RGCGCY 1 cut(s) 477
HaeIII GGCC 2 cut(s) 5, 554
HapII CCGG 1 cut(s) 6
HhaI GCGC 1 cut(s) 476
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 4 cut(s) 65, 101, 170, 506
Hin6I GCGC 1 cut(s) 474
HinP1I GCGC 1 cut(s) 474
HindIII AAGCTT 2 cut(s) 366, 612
HinfI GANTC 5 cut(s) 163, 212, 232, 238, 260
HpaII CCGG 1 cut(s) 6
Hpy188I TCNGA 5 cut(s) 105, 135, 157, 237, 565
Hpy188III TCNNGA 1 cut(s) 582
HpyCH4III ACNGT 5 cut(s) 53, 178, 206, 251, 463
HpyCH4IV ACGT 1 cut(s) 401
HpyCH4V TGCA 8 cut(s) 272, 292, 339, 433, 440, 457, 506, 593
HpyF10VI GCNNNNNNNGC 1 cut(s) 187
HpyF3I CTNAG 4 cut(s) 104, 119, 564, 615
HpySE526I ACGT 1 cut(s) 401
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 4 cut(s) 65, 101, 170, 506
HspAI GCGC 1 cut(s) 474
KasI GGCGCC 1 cut(s) 473
Kzo9I GATC 1 cut(s) 499
LmnI GCTCC 1 cut(s) 324
LpnPI CCDG 8 cut(s) 19, 105, 141, 252, 342, 494, 535, 555
LweI GCATC 2 cut(s) 298, 525
MaeII ACGT 1 cut(s) 401
MalI GATC 1 cut(s) 501
MboI GATC 1 cut(s) 499
MboII GAAGA 2 cut(s) 287, 374
MfeI CAATTG 1 cut(s) 594
MhlI GDGCHC 2 cut(s) 183, 329
MluCI AATT 5 cut(s) 151, 284, 315, 427, 594
Mly113I GGCGCC 1 cut(s) 474
MlyI GAGTC 2 cut(s) 221, 226
MnlI CCTC 8 cut(s) 60, 99, 193, 402, 487, 522, 526, 580
MseI TTAA 3 cut(s) 12, 87, 372
MspCI CTTAAG 2 cut(s) 11, 86
MspI CCGG 1 cut(s) 6
MunI CAATTG 1 cut(s) 594
Mva1269I GAATGC 1 cut(s) 420
MwoI GCNNNNNNNGC 1 cut(s) 187
NarI GGCGCC 1 cut(s) 474
NdeI CATATG 1 cut(s) 221
NdeII GATC 1 cut(s) 499
NlaIII CATG 4 cut(s) 65, 101, 170, 506
NlaIV GGNNCC 2 cut(s) 326, 475
NmeAIII GCCGAG 1 cut(s) 580
PctI GAATGC 1 cut(s) 420
PfeI GAWTC 3 cut(s) 163, 238, 260
PleI GAGTC 2 cut(s) 220, 226
PluTI GGCGCC 1 cut(s) 477
PpsI GAGTC 2 cut(s) 220, 226
Psp1406I AACGTT 1 cut(s) 401
PspN4I GGNNCC 2 cut(s) 326, 475
PspPI GGNCC 1 cut(s) 552
SaqAI TTAA 3 cut(s) 12, 87, 372
Sau3AI GATC 1 cut(s) 499
Sau96I GGNCC 1 cut(s) 552
SchI GAGTC 2 cut(s) 221, 226
SduI GDGCHC 2 cut(s) 183, 329
SetI ASST 5 cut(s) 71, 370, 404, 591, 616
SfaNI GCATC 2 cut(s) 298, 525
SfcI CTRYAG 1 cut(s) 174
SfoI GGCGCC 1 cut(s) 475
SmlI CTYRAG 3 cut(s) 11, 18, 86
SmoI CTYRAG 3 cut(s) 11, 18, 86
Sse9I AATT 5 cut(s) 151, 284, 315, 427, 594
SspDI GGCGCC 1 cut(s) 473
StyI CCWWGG 1 cut(s) 320
TaaI ACNGT 5 cut(s) 53, 178, 206, 251, 463
TaiI ACGT 1 cut(s) 404
TaqI TCGA 1 cut(s) 581
TasI AATT 5 cut(s) 151, 284, 315, 427, 594
TfiI GAWTC 3 cut(s) 163, 238, 260
Tru1I TTAA 3 cut(s) 12, 87, 372
Tru9I TTAA 3 cut(s) 12, 87, 372
TscAI CASTG 3 cut(s) 183, 256, 513
TspDTI ATGAA 1 cut(s) 50
TspRI CASTG 3 cut(s) 183, 256, 513
Vha464I CTTAAG 2 cut(s) 11, 86
XapI RAATTY 2 cut(s) 151, 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.