FvH4_3g38390

Pleckstrin homology domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009844
Physical Location & Seq
Forward (+)
32792866 .. 32795002
2137 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g38390.t1

Sequence Viewer

Length: 444 bp
ATGGAAGGACTGTGGCGGGCAGCGACGGGCCAGGACCCGAGCCCAGAAGACTACACGGGCATCGAGTACTGGTCCAACCCCGAACGCGCCGCCTGGCTGACGAAGCAAGGCGAGTACATCAAGACCTGGCGCCGCCGCTGGTTCGTCCTCAAGCAAGGCAAGCTCTTCTGGTTCAAAGACTCCCACGTGACCCCCTCCACCACCCCACGTGGCGTCATCCCCGTCGGCACGTGCCTCACCGTCAAAGGCGCCGAGGACGTCCTCCACAAGCCCTGCGCCTTCGAGCTCTCCACCACCACACACGACACCATGTACTTCATCGCCGATACCGAGAAGGAGAAGGAGGAGTGGATCAACTCCATCGGACGGTCGTTGGTCCAGCACTCCAGATCGCTCGCCGATTCCGAGGTCGTCGATTACGATAACCGCCGAAATGCAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PH

Protein Analysis

148

Amino Acids

17.0

Weight (kDa)

5.84

Isoelectric Point (pI)

50.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 30 - 123 2.4e-19 PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
AccB1I GGYRCC 2 cut(s) 129, 248
AccII CGCG 1 cut(s) 87
AciI CCGC 5 cut(s) 16, 90, 133, 136, 427
AclWI GGATC 1 cut(s) 359
AcvI CACGTG 3 cut(s) 187, 209, 231
AcyI GRCGYC 4 cut(s) 130, 213, 249, 258
AfaI GTAC 3 cut(s) 68, 116, 314
AfiI CCNNNNNNNGG 1 cut(s) 366
AgsI TTSAA 1 cut(s) 175
AjnI CCWGG 3 cut(s) 30, 92, 125
AluBI AGCT 2 cut(s) 163, 286
AluI AGCT 2 cut(s) 163, 286
Alw21I GWGCWC 1 cut(s) 288
AlwI GGATC 1 cut(s) 359
Ama87I CYCGRG 1 cut(s) 37
AoxI GGCC 1 cut(s) 28
ApeKI GCWGC 1 cut(s) 20
AspLEI GCGC 4 cut(s) 89, 132, 251, 278
AspS9I GGNCC 4 cut(s) 28, 34, 72, 376
AsuHPI GGTGA 1 cut(s) 229
AvaI CYCGRG 1 cut(s) 37
AvaII GGWCC 3 cut(s) 34, 72, 376
BanI GGYRCC 2 cut(s) 129, 248
BanII GRGCYC 2 cut(s) 44, 288
BbrPI CACGTG 3 cut(s) 187, 209, 231
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 288
BbvI GCAGC 1 cut(s) 32
BccI CCATC 1 cut(s) 368
BciT130I CCWGG 3 cut(s) 32, 94, 127
BfoI RGCGCY 2 cut(s) 133, 252
BisI GCNGC 4 cut(s) 21, 90, 133, 136
BlsI GCNGC 4 cut(s) 22, 91, 134, 137
BmcAI AGTACT 1 cut(s) 68
Bme1390I CCNGG 3 cut(s) 32, 94, 127
Bme18I GGWCC 3 cut(s) 34, 72, 376
BmeT110I CYCGRG 1 cut(s) 37
BmgT120I GGNCC 4 cut(s) 28, 34, 72, 376
BmiI GGNNCC 3 cut(s) 36, 131, 250
BmrFI CCNGG 3 cut(s) 32, 94, 127
BmsI GCATC 1 cut(s) 69
BpiI GAAGAC 1 cut(s) 54
BpmI CTGGAG 1 cut(s) 370
BpuEI CTTGAG 1 cut(s) 134
BsaAI YACGTR 3 cut(s) 187, 209, 231
BsaHI GRCGYC 4 cut(s) 130, 213, 249, 258
BsaJI CCNNGG 2 cut(s) 252, 405
Bsc4I CCNNNNNNNGG 1 cut(s) 366
Bse1I ACTGG 1 cut(s) 74
BseBI CCWGG 3 cut(s) 32, 94, 127
BseDI CCNNGG 2 cut(s) 252, 405
BseGI GGATG 1 cut(s) 216
BseLI CCNNNNNNNGG 1 cut(s) 366
BseNI ACTGG 1 cut(s) 74
BseRI GAGGAG 1 cut(s) 359
BseXI GCAGC 1 cut(s) 32
Bsh1236I CGCG 1 cut(s) 87
Bsh1285I CGRYCG 1 cut(s) 371
BshFI GGCC 1 cut(s) 30
BshNI GGYRCC 2 cut(s) 129, 248
BsiEI CGRYCG 1 cut(s) 371
BsiHKAI GWGCWC 1 cut(s) 288
BsiHKCI CYCGRG 1 cut(s) 37
BslI CCNNNNNNNGG 1 cut(s) 366
BsnI GGCC 1 cut(s) 30
BsoBI CYCGRG 1 cut(s) 37
Bsp1286I GDGCHC 2 cut(s) 44, 288
Bsp143I GATC 2 cut(s) 351, 389
BspACI CCGC 5 cut(s) 16, 90, 133, 136, 427
BspANI GGCC 1 cut(s) 30
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 3 cut(s) 36, 131, 250
BspPI GGATC 1 cut(s) 359
BspQI GCTCTTC 1 cut(s) 170
BspT107I GGYRCC 2 cut(s) 129, 248
BsrI ACTGG 1 cut(s) 74
BssECI CCNNGG 2 cut(s) 252, 405
BssMI GATC 2 cut(s) 351, 389
BssNI GRCGYC 4 cut(s) 130, 213, 249, 258
Bst2UI CCWGG 3 cut(s) 32, 94, 127
Bst4CI ACNGT 3 cut(s) 12, 241, 369
Bst6I CTCTTC 1 cut(s) 170
BstACI GRCGYC 4 cut(s) 130, 213, 249, 258
BstBAI YACGTR 3 cut(s) 187, 209, 231
BstC8I GCNNGC 3 cut(s) 18, 161, 396
BstF5I GGATG 1 cut(s) 216
BstFNI CGCG 1 cut(s) 87
BstH2I RGCGCY 2 cut(s) 133, 252
BstHHI GCGC 4 cut(s) 89, 132, 251, 278
BstKTI GATC 2 cut(s) 354, 392
BstMBI GATC 2 cut(s) 351, 389
BstMCI CGRYCG 1 cut(s) 371
BstMWI GCNNNNNNNGC 2 cut(s) 103, 160
BstNI CCWGG 3 cut(s) 32, 94, 127
BstSCI CCNGG 3 cut(s) 30, 92, 125
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 1 cut(s) 32
BstV2I GAAGAC 1 cut(s) 54
BsuRI GGCC 1 cut(s) 30
BtgZI GCGATG 1 cut(s) 304
BtsCI GGATG 1 cut(s) 216
Cac8I GCNNGC 3 cut(s) 18, 161, 396
CfoI GCGC 4 cut(s) 89, 132, 251, 278
Cfr13I GGNCC 4 cut(s) 28, 34, 72, 376
CseI GACGC 1 cut(s) 202
Csp6I GTAC 3 cut(s) 67, 115, 313
CviAII CATG 1 cut(s) 310
CviJI RGCY 6 cut(s) 30, 42, 97, 163, 271, 286
CviKI_1 RGCY 6 cut(s) 30, 42, 97, 163, 271, 286
CviQI GTAC 3 cut(s) 67, 115, 313
DinI GGCGCC 2 cut(s) 131, 250
DpnI GATC 2 cut(s) 353, 391
DpnII GATC 2 cut(s) 351, 389
Eam1104I CTCTTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 170
Ecl136II GAGCTC 1 cut(s) 286
Eco24I GRGCYC 2 cut(s) 44, 288
Eco47I GGWCC 3 cut(s) 34, 72, 376
Eco53kI GAGCTC 1 cut(s) 286
Eco72I CACGTG 3 cut(s) 187, 209, 231
Eco88I CYCGRG 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 286
EcoO109I RGGNCCY 1 cut(s) 34
EcoRII CCWGG 3 cut(s) 30, 92, 125
EcoT38I GRGCYC 2 cut(s) 44, 288
EgeI GGCGCC 2 cut(s) 131, 250
EheI GGCGCC 2 cut(s) 131, 250
FaeI CATG 1 cut(s) 313
FaiI YATR 1 cut(s) 311
FatI CATG 1 cut(s) 309
FauI CCCGC 1 cut(s) 9
Fnu4HI GCNGC 4 cut(s) 21, 90, 133, 136
FokI GGATG 1 cut(s) 203
FriOI GRGCYC 2 cut(s) 44, 288
Fsp4HI GCNGC 4 cut(s) 21, 90, 133, 136
GlaI GCGC 4 cut(s) 88, 131, 250, 277
GluI GCNGC 4 cut(s) 21, 90, 133, 136
GsuI CTGGAG 1 cut(s) 370
HaeII RGCGCY 2 cut(s) 133, 252
HaeIII GGCC 1 cut(s) 30
HgaI GACGC 1 cut(s) 202
HhaI GCGC 4 cut(s) 89, 132, 251, 278
Hin1I GRCGYC 4 cut(s) 130, 213, 249, 258
Hin1II CATG 1 cut(s) 313
Hin6I GCGC 4 cut(s) 87, 130, 249, 276
HinP1I GCGC 4 cut(s) 87, 130, 249, 276
HinfI GANTC 2 cut(s) 179, 401
HphI GGTGA 1 cut(s) 229
Hpy188I TCNGA 2 cut(s) 365, 406
Hpy188III TCNNGA 2 cut(s) 121, 387
Hpy99I CGWCG 3 cut(s) 28, 227, 416
HpyAV CCTTC 3 cut(s) 289, 328, 334
HpyCH4III ACNGT 3 cut(s) 12, 241, 369
HpyCH4IV ACGT 4 cut(s) 186, 208, 230, 258
HpyCH4V TGCA 1 cut(s) 437
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 160
HpySE526I ACGT 4 cut(s) 186, 208, 230, 258
Hsp92I GRCGYC 4 cut(s) 130, 213, 249, 258
Hsp92II CATG 1 cut(s) 313
HspAI GCGC 4 cut(s) 87, 130, 249, 276
KasI GGCGCC 2 cut(s) 129, 248
Kzo9I GATC 2 cut(s) 351, 389
LguI GCTCTTC 1 cut(s) 170
Lsp1109I GCAGC 1 cut(s) 32
LweI GCATC 1 cut(s) 69
MaeII ACGT 4 cut(s) 186, 208, 230, 258
MaeIII GTNAC 1 cut(s) 187
MalI GATC 2 cut(s) 353, 391
MboI GATC 2 cut(s) 351, 389
MboII GAAGA 2 cut(s) 59, 157
MhlI GDGCHC 2 cut(s) 44, 288
Mly113I GGCGCC 2 cut(s) 130, 249
MlyI GAGTC 1 cut(s) 173
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 7 cut(s) 158, 205, 245, 247, 272, 337, 400
MspA1I CMGCKG 1 cut(s) 138
MspR9I CCNGG 3 cut(s) 32, 94, 127
MvaI CCWGG 3 cut(s) 32, 94, 127
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 2 cut(s) 103, 160
NarI GGCGCC 2 cut(s) 130, 249
NdeII GATC 2 cut(s) 351, 389
NlaIII CATG 1 cut(s) 313
NlaIV GGNNCC 3 cut(s) 36, 131, 250
NmeAIII GCCGAG 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 187
PciSI GCTCTTC 1 cut(s) 170
PcsI WCGNNNNNNNCGW 3 cut(s) 327, 402, 417
PfeI GAWTC 1 cut(s) 401
PkrI GCNGC 4 cut(s) 22, 91, 134, 137
PleI GAGTC 1 cut(s) 173
PluTI GGCGCC 2 cut(s) 133, 252
PmaCI CACGTG 3 cut(s) 187, 209, 231
PmlI CACGTG 3 cut(s) 187, 209, 231
PpsI GAGTC 1 cut(s) 173
Ppu21I YACGTR 3 cut(s) 187, 209, 231
PpuMI RGGWCCY 1 cut(s) 34
Psp124BI GAGCTC 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 34
Psp6I CCWGG 3 cut(s) 30, 92, 125
PspCI CACGTG 3 cut(s) 187, 209, 231
PspGI CCWGG 3 cut(s) 30, 92, 125
PspN4I GGNNCC 3 cut(s) 36, 131, 250
PspPI GGNCC 4 cut(s) 28, 34, 72, 376
PspPPI RGGWCCY 1 cut(s) 34
RsaI GTAC 3 cut(s) 68, 116, 314
RsaNI GTAC 3 cut(s) 67, 115, 313
SacI GAGCTC 1 cut(s) 288
SapI GCTCTTC 1 cut(s) 170
SatI GCNGC 4 cut(s) 21, 90, 133, 136
Sau3AI GATC 2 cut(s) 351, 389
Sau96I GGNCC 4 cut(s) 28, 34, 72, 376
ScaI AGTACT 1 cut(s) 68
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 3 cut(s) 32, 94, 127
SduI GDGCHC 2 cut(s) 44, 288
SetI ASST 8 cut(s) 128, 165, 189, 211, 233, 261, 288, 411
SfaNI GCATC 1 cut(s) 69
SfoI GGCGCC 2 cut(s) 131, 250
SinI GGWCC 3 cut(s) 34, 72, 376
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
SsiI CCGC 5 cut(s) 16, 90, 133, 136, 427
SspDI GGCGCC 2 cut(s) 129, 248
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 3 cut(s) 30, 92, 125
TaaI ACNGT 3 cut(s) 12, 241, 369
TaiI ACGT 4 cut(s) 189, 211, 233, 261
TaqI TCGA 3 cut(s) 63, 282, 414
TatI WGTACW 3 cut(s) 66, 114, 312
TauI GCSGC 3 cut(s) 92, 135, 138
TfiI GAWTC 1 cut(s) 401
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 1 cut(s) 20
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 1 cut(s) 307
VpaK11BI GGWCC 3 cut(s) 34, 72, 376
ZraI GACGTC 1 cut(s) 259
ZrmI AGTACT 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.