MD11G1077100.v1.1

Pleckstrin homology domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0009844
Physical Location & Seq
Reverse (-)
6533487 .. 6533924
438 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1077100.v1.1.491

Sequence Viewer

Length: 438 bp
ATGGAGGGCCTGTGGCGGGCGGCTACGGGCCAAGACCCGAGTCCGGAAGACTACACCGGAATCGAGTTCTGGTCCAACCCGGAACGCGCCACCTGGCTGACCAAACAAGGCGATTACATCAAGACGTGGCGGCGCCGCTGGTTCGTCTTGAAACAGGGGAAGCTGTTCTGGTTCAAGGACAGTTACGTTACCCGTTCGTCGACGCCGCGCGGCGTGATTCCGGTCGGCACGTGCCTGACGGTGAAGGGCGCCGAGGACGTGGTGCACAAGCCCTGCGCCTTCGAGCTCTCCACCACTAATCATGATACGATGTACTTCATCGCCGATTCGGAGAAAGAGAAGGAGGAGTGGATCAACTCGATCGGACGGTCCATCGTCCAGCACTCCAGATCGCTCGCGGATTCTGAGGTCGTCGATTACGATAGCAGCCGACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PH

Protein Analysis

146

Amino Acids

16.77

Weight (kDa)

5.93

Isoelectric Point (pI)

55.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 30 - 125 3.8e-20 PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 132, 248
AccI GTMKAC 1 cut(s) 200
AccII CGCG 4 cut(s) 87, 208, 210, 398
AccIII TCCGGA 1 cut(s) 43
AciI CCGC 7 cut(s) 16, 20, 130, 136, 206, 210, 398
AclWI GGATC 1 cut(s) 359
AcvI CACGTG 1 cut(s) 231
AcyI GRCGYC 3 cut(s) 133, 203, 249
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 2 cut(s) 16, 43
AgsI TTSAA 2 cut(s) 151, 175
AjiI CACGTC 2 cut(s) 126, 259
AjnI CCWGG 1 cut(s) 92
AluBI AGCT 2 cut(s) 163, 286
AluI AGCT 2 cut(s) 163, 286
Alw21I GWGCWC 2 cut(s) 267, 288
Alw44I GTGCAC 1 cut(s) 263
AlwI GGATC 1 cut(s) 359
Ama87I CYCGRG 1 cut(s) 37
Aor13HI TCCGGA 1 cut(s) 43
AoxI GGCC 2 cut(s) 7, 28
ApaLI GTGCAC 1 cut(s) 263
ApeKI GCWGC 1 cut(s) 426
Asp700I GAANNNNTTC 1 cut(s) 164
AspLEI GCGC 5 cut(s) 89, 135, 210, 251, 278
AspS9I GGNCC 4 cut(s) 7, 28, 72, 369
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 253
AvaI CYCGRG 1 cut(s) 37
AvaII GGWCC 2 cut(s) 72, 369
BaeGI GKGCMC 1 cut(s) 267
BanI GGYRCC 2 cut(s) 132, 248
BanII GRGCYC 1 cut(s) 288
BbrPI CACGTG 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 2 cut(s) 267, 288
BccI CCATC 1 cut(s) 380
BciT130I CCWGG 1 cut(s) 94
BcnI CCSGG 1 cut(s) 80
BfoI RGCGCY 2 cut(s) 136, 252
BisI GCNGC 6 cut(s) 21, 131, 136, 206, 211, 427
BlsI GCNGC 6 cut(s) 22, 132, 137, 207, 212, 428
Bme1390I CCNGG 2 cut(s) 80, 94
Bme18I GGWCC 2 cut(s) 72, 369
BmeT110I CYCGRG 1 cut(s) 37
BmgBI CACGTC 2 cut(s) 126, 259
BmgT120I GGNCC 4 cut(s) 7, 28, 72, 369
BmiI GGNNCC 2 cut(s) 134, 250
BmrFI CCNGG 2 cut(s) 80, 94
BoxI GACNNNNGTC 1 cut(s) 39
BpiI GAAGAC 1 cut(s) 54
BpmI CTGGAG 1 cut(s) 370
BpuMI CCSGG 1 cut(s) 80
BsaAI YACGTR 1 cut(s) 231
BsaHI GRCGYC 3 cut(s) 133, 203, 249
BsaJI CCNNGG 1 cut(s) 252
BsaWI WCCGGW 3 cut(s) 43, 56, 220
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 43
BseAI TCCGGA 1 cut(s) 43
BseBI CCWGG 1 cut(s) 94
BseDI CCNNGG 1 cut(s) 252
BseLI CCNNNNNNNGG 2 cut(s) 16, 43
BseMII CTCAG 1 cut(s) 396
BseRI GAGGAG 1 cut(s) 359
BseSI GKGCMC 1 cut(s) 267
Bsh1236I CGCG 4 cut(s) 87, 208, 210, 398
Bsh1285I CGRYCG 2 cut(s) 225, 363
BshFI GGCC 2 cut(s) 9, 30
BshNI GGYRCC 2 cut(s) 132, 248
BsiEI CGRYCG 2 cut(s) 225, 363
BsiHKAI GWGCWC 2 cut(s) 267, 288
BsiHKCI CYCGRG 1 cut(s) 37
BsiSI CCGG 4 cut(s) 44, 57, 80, 221
BslI CCNNNNNNNGG 2 cut(s) 16, 43
BsnI GGCC 2 cut(s) 9, 30
BsoBI CYCGRG 1 cut(s) 37
Bsp1286I GDGCHC 2 cut(s) 267, 288
Bsp13I TCCGGA 1 cut(s) 43
Bsp143I GATC 3 cut(s) 351, 360, 389
BspACI CCGC 7 cut(s) 16, 20, 130, 136, 206, 210, 398
BspANI GGCC 2 cut(s) 9, 30
BspCNI CTCAG 1 cut(s) 397
BspEI TCCGGA 1 cut(s) 43
BspFNI CGCG 4 cut(s) 87, 208, 210, 398
BspHI TCATGA 1 cut(s) 301
BspLI GGNNCC 2 cut(s) 134, 250
BspPI GGATC 1 cut(s) 359
BspT107I GGYRCC 2 cut(s) 132, 248
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 3 cut(s) 351, 360, 389
BssNI GRCGYC 3 cut(s) 133, 203, 249
Bst2UI CCWGG 1 cut(s) 94
Bst4CI ACNGT 4 cut(s) 182, 241, 369, 435
BstACI GRCGYC 3 cut(s) 133, 203, 249
BstBAI YACGTR 1 cut(s) 231
BstC8I GCNNGC 2 cut(s) 18, 396
BstDEI CTNAG 1 cut(s) 405
BstFNI CGCG 4 cut(s) 87, 208, 210, 398
BstH2I RGCGCY 2 cut(s) 136, 252
BstHHI GCGC 5 cut(s) 89, 135, 210, 251, 278
BstKTI GATC 3 cut(s) 354, 363, 392
BstMBI GATC 3 cut(s) 351, 360, 389
BstMCI CGRYCG 2 cut(s) 225, 363
BstNI CCWGG 1 cut(s) 94
BstPAI GACNNNNGTC 1 cut(s) 39
BstSCI CCNGG 2 cut(s) 78, 92
BstSLI GKGCMC 1 cut(s) 267
BstUI CGCG 4 cut(s) 87, 208, 210, 398
BstV2I GAAGAC 1 cut(s) 54
BsuRI GGCC 2 cut(s) 9, 30
BtgZI GCGATG 1 cut(s) 304
BtrI CACGTC 2 cut(s) 126, 259
Cac8I GCNNGC 2 cut(s) 18, 396
CciI TCATGA 1 cut(s) 301
CfoI GCGC 5 cut(s) 89, 135, 210, 251, 278
Cfr13I GGNCC 4 cut(s) 7, 28, 72, 369
CseI GACGC 1 cut(s) 211
Csp6I GTAC 1 cut(s) 313
CviAII CATG 1 cut(s) 302
CviJI RGCY 8 cut(s) 9, 23, 30, 97, 163, 271, 286, 429
CviKI_1 RGCY 8 cut(s) 9, 23, 30, 97, 163, 271, 286, 429
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 1 cut(s) 405
DinI GGCGCC 2 cut(s) 134, 250
DpnI GATC 3 cut(s) 353, 362, 391
DpnII GATC 3 cut(s) 351, 360, 389
Ecl136II GAGCTC 1 cut(s) 286
Eco24I GRGCYC 1 cut(s) 288
Eco47I GGWCC 2 cut(s) 72, 369
Eco53kI GAGCTC 1 cut(s) 286
Eco72I CACGTG 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 286
EcoO109I RGGNCCY 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 92
EcoT38I GRGCYC 1 cut(s) 288
EgeI GGCGCC 2 cut(s) 134, 250
EheI GGCGCC 2 cut(s) 134, 250
FaeI CATG 1 cut(s) 305
FaiI YATR 1 cut(s) 303
FatI CATG 1 cut(s) 301
FauI CCCGC 1 cut(s) 9
FblI GTMKAC 1 cut(s) 200
Fnu4HI GCNGC 6 cut(s) 21, 131, 136, 206, 211, 427
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 6 cut(s) 21, 131, 136, 206, 211, 427
GlaI GCGC 5 cut(s) 88, 134, 209, 250, 277
GluI GCNGC 6 cut(s) 21, 131, 136, 206, 211, 427
GsuI CTGGAG 1 cut(s) 370
HaeII RGCGCY 2 cut(s) 136, 252
HaeIII GGCC 2 cut(s) 9, 30
HapII CCGG 4 cut(s) 44, 57, 80, 221
HgaI GACGC 1 cut(s) 211
HhaI GCGC 5 cut(s) 89, 135, 210, 251, 278
Hin1I GRCGYC 3 cut(s) 133, 203, 249
Hin1II CATG 1 cut(s) 305
Hin6I GCGC 5 cut(s) 87, 133, 208, 249, 276
HinP1I GCGC 5 cut(s) 87, 133, 208, 249, 276
HincII GTYRAC 1 cut(s) 201
HindII GTYRAC 1 cut(s) 201
HinfI GANTC 5 cut(s) 40, 60, 217, 326, 401
HpaII CCGG 4 cut(s) 44, 57, 80, 221
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 2 cut(s) 201, 265
Hpy188I TCNGA 3 cut(s) 331, 365, 406
Hpy188III TCNNGA 5 cut(s) 44, 121, 148, 302, 387
Hpy8I GTNNAC 2 cut(s) 201, 265
Hpy99I CGWCG 3 cut(s) 202, 205, 416
HpyAV CCTTC 3 cut(s) 238, 289, 334
HpyCH4III ACNGT 4 cut(s) 182, 241, 369, 435
HpyCH4IV ACGT 4 cut(s) 125, 186, 230, 258
HpyCH4V TGCA 1 cut(s) 265
HpyF3I CTNAG 1 cut(s) 405
HpySE526I ACGT 4 cut(s) 125, 186, 230, 258
Hsp92I GRCGYC 3 cut(s) 133, 203, 249
Hsp92II CATG 1 cut(s) 305
HspAI GCGC 5 cut(s) 87, 133, 208, 249, 276
KasI GGCGCC 2 cut(s) 132, 248
Kpn2I TCCGGA 1 cut(s) 43
Kzo9I GATC 3 cut(s) 351, 360, 389
MaeII ACGT 4 cut(s) 125, 186, 230, 258
MaeIII GTNAC 2 cut(s) 182, 187
MalI GATC 3 cut(s) 353, 362, 391
MboI GATC 3 cut(s) 351, 360, 389
MboII GAAGA 1 cut(s) 59
MhlI GDGCHC 2 cut(s) 267, 288
Mly113I GGCGCC 2 cut(s) 133, 249
MlyI GAGTC 1 cut(s) 49
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 3 cut(s) 247, 337, 400
MroI TCCGGA 1 cut(s) 43
MroXI GAANNNNTTC 1 cut(s) 164
MspA1I CMGCKG 1 cut(s) 138
MspI CCGG 4 cut(s) 44, 57, 80, 221
MspR9I CCNGG 2 cut(s) 80, 94
MvaI CCWGG 1 cut(s) 94
MvnI CGCG 4 cut(s) 87, 208, 210, 398
NarI GGCGCC 2 cut(s) 133, 249
NciI CCSGG 1 cut(s) 80
NdeII GATC 3 cut(s) 351, 360, 389
NlaIII CATG 1 cut(s) 305
NlaIV GGNNCC 2 cut(s) 134, 250
NmeAIII GCCGAG 1 cut(s) 277
PagI TCATGA 1 cut(s) 301
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PdmI GAANNNNTTC 1 cut(s) 164
PfeI GAWTC 4 cut(s) 60, 217, 326, 401
PkrI GCNGC 6 cut(s) 22, 132, 137, 207, 212, 428
Ple19I CGATCG 1 cut(s) 363
PleI GAGTC 1 cut(s) 48
PluTI GGCGCC 2 cut(s) 136, 252
PmaCI CACGTG 1 cut(s) 231
PmlI CACGTG 1 cut(s) 231
PpsI GAGTC 1 cut(s) 48
Ppu21I YACGTR 1 cut(s) 231
PshAI GACNNNNGTC 1 cut(s) 39
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 92
PspCI CACGTG 1 cut(s) 231
PspGI CCWGG 1 cut(s) 92
PspN4I GGNNCC 2 cut(s) 134, 250
PspPI GGNCC 4 cut(s) 7, 28, 72, 369
PvuI CGATCG 1 cut(s) 363
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
SacI GAGCTC 1 cut(s) 288
SalI GTCGAC 1 cut(s) 199
SatI GCNGC 6 cut(s) 21, 131, 136, 206, 211, 427
Sau3AI GATC 3 cut(s) 351, 360, 389
Sau96I GGNCC 4 cut(s) 7, 28, 72, 369
SchI GAGTC 1 cut(s) 49
ScrFI CCNGG 2 cut(s) 80, 94
SduI GDGCHC 2 cut(s) 267, 288
SetI ASST 8 cut(s) 95, 128, 165, 189, 233, 261, 288, 411
SfoI GGCGCC 2 cut(s) 134, 250
SgrDI CGTCGACG 1 cut(s) 199
SinI GGWCC 2 cut(s) 72, 369
SsiI CCGC 7 cut(s) 16, 20, 130, 136, 206, 210, 398
SspDI GGCGCC 2 cut(s) 132, 248
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 2 cut(s) 78, 92
TaaI ACNGT 4 cut(s) 182, 241, 369, 435
TaiI ACGT 4 cut(s) 128, 189, 233, 261
TaqI TCGA 5 cut(s) 63, 200, 282, 359, 414
TatI WGTACW 1 cut(s) 312
TauI GCSGC 5 cut(s) 23, 133, 138, 208, 213
TfiI GAWTC 4 cut(s) 60, 217, 326, 401
TseI GCWGC 1 cut(s) 426
TspDTI ATGAA 1 cut(s) 307
VneI GTGCAC 1 cut(s) 263
VpaK11BI GGWCC 2 cut(s) 72, 369
XmiI GTMKAC 1 cut(s) 200
XmnI GAANNNNTTC 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.