Rmu_sc0000853.1_g000005

Pleckstrin homology domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000853.1
OG0009844
Physical Location & Seq
Forward (+)
13276 .. 13881
606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000853.1_g000005.1.cds

Sequence Viewer

Length: 606 bp
atgtgcaagattgtggggtccaacttcttatactccagcacagagaacgacagcgtattatggcacccagctaatcacgaaccatattccgttccgcgatcccaaaaaaggcaactgaaactcccaaaccctctctcttgtctcttcccatctccgtcttctcagctcttgatggagggtctgtggcgggccgcgacgggccaggacccaagcccagaggactacaccggcatcgaattctggtccaaccccgaacgcgccgcttggctcaccaagcaaggcgagtacatcaagacctggcgccgccgctggttcgtcctcaagcaaggcaagctcttctggttcaaggactcccacgtcaccccctcctccaccccacgcggcgtcatccccgtcggcacctgcctcaccgtcaagggcgccgaggacgtcctccacaagccctgcgccttcgagctctccaccaccacccacgacaccatgtacttcatcgccgatactgagaaggagaaggaggagtggatcaactccatcggacggtcattagtccagcactccagatcgctcgccgattccgaggtcgtcgattacgataaccgccggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PH

Protein Analysis

201

Amino Acids

23.08

Weight (kDa)

7.01

Isoelectric Point (pI)

61.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 410
AasI GACNNNNNNGTC 1 cut(s) 356
AatII GACGTC 1 cut(s) 432
Acc36I ACCTGC 1 cut(s) 410
AccB1I GGYRCC 4 cut(s) 63, 300, 398, 419
AccII CGCG 4 cut(s) 97, 194, 258, 381
AciI CCGC 8 cut(s) 95, 187, 192, 261, 304, 307, 381, 598
AclWI GGATC 2 cut(s) 93, 530
AcsI RAATTY 1 cut(s) 236
AcyI GRCGYC 4 cut(s) 301, 384, 420, 429
AfaI GTAC 2 cut(s) 287, 485
AfiI CCNNNNNNNGG 2 cut(s) 108, 537
AgsI TTSAA 1 cut(s) 346
AjiI CACGTC 1 cut(s) 358
AjnI CCWGG 2 cut(s) 201, 296
AluBI AGCT 4 cut(s) 71, 166, 334, 457
AluI AGCT 4 cut(s) 71, 166, 334, 457
Alw21I GWGCWC 1 cut(s) 459
Alw26I GTCTC 1 cut(s) 146
AlwI GGATC 2 cut(s) 93, 530
AoxI GGCC 2 cut(s) 189, 199
ApoI RAATTY 1 cut(s) 236
AspLEI GCGC 4 cut(s) 260, 303, 422, 449
AspS9I GGNCC 5 cut(s) 18, 189, 199, 205, 243
AsuHPI GGTGA 3 cut(s) 262, 352, 400
AvaII GGWCC 3 cut(s) 18, 205, 243
BanI GGYRCC 4 cut(s) 63, 300, 398, 419
BanII GRGCYC 1 cut(s) 459
BbsI GAAGAC 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 459
BccI CCATC 3 cut(s) 157, 166, 539
BciT130I CCWGG 2 cut(s) 203, 298
BcoDI GTCTC 1 cut(s) 146
BfoI RGCGCY 2 cut(s) 304, 423
BfuAI ACCTGC 1 cut(s) 410
BisI GCNGC 5 cut(s) 192, 261, 304, 307, 382
BlsI GCNGC 5 cut(s) 193, 262, 305, 308, 383
Bme1390I CCNGG 2 cut(s) 203, 298
Bme18I GGWCC 3 cut(s) 18, 205, 243
BmgBI CACGTC 1 cut(s) 358
BmgT120I GGNCC 5 cut(s) 18, 189, 199, 205, 243
BmiI GGNNCC 6 cut(s) 19, 65, 207, 302, 400, 421
BmrFI CCNGG 2 cut(s) 203, 298
BmsI GCATC 1 cut(s) 240
BpiI GAAGAC 1 cut(s) 150
BpmI CTGGAG 2 cut(s) 19, 541
BpuEI CTTGAG 1 cut(s) 305
BsaHI GRCGYC 4 cut(s) 301, 384, 420, 429
BsaJI CCNNGG 2 cut(s) 423, 576
BsaXI ACNNNNNCTCC 4 cut(s) 105, 135, 167, 197
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 537
Bse118I RCCGGY 2 cut(s) 227, 600
BseBI CCWGG 2 cut(s) 203, 298
BseDI CCNNGG 2 cut(s) 423, 576
BseGI GGATG 1 cut(s) 387
BseLI CCNNNNNNNGG 2 cut(s) 108, 537
BseMII CTCAG 2 cut(s) 176, 492
BseRI GAGGAG 2 cut(s) 358, 530
BseYI CCCAGC 1 cut(s) 67
Bsh1236I CGCG 4 cut(s) 97, 194, 258, 381
BshFI GGCC 2 cut(s) 191, 201
BshNI GGYRCC 4 cut(s) 63, 300, 398, 419
BsiHKAI GWGCWC 1 cut(s) 459
BsiSI CCGG 2 cut(s) 228, 601
BslI CCNNNNNNNGG 2 cut(s) 108, 537
BsmAI GTCTC 1 cut(s) 146
BsnI GGCC 2 cut(s) 191, 201
Bsp1286I GDGCHC 1 cut(s) 459
Bsp143I GATC 3 cut(s) 98, 522, 560
BspACI CCGC 8 cut(s) 95, 187, 192, 261, 304, 307, 381, 598
BspANI GGCC 2 cut(s) 191, 201
BspCNI CTCAG 2 cut(s) 175, 493
BspFNI CGCG 4 cut(s) 97, 194, 258, 381
BspLI GGNNCC 6 cut(s) 19, 65, 207, 302, 400, 421
BspMI ACCTGC 1 cut(s) 410
BspPI GGATC 2 cut(s) 93, 530
BspQI GCTCTTC 1 cut(s) 341
BspT107I GGYRCC 4 cut(s) 63, 300, 398, 419
BsrFI RCCGGY 2 cut(s) 227, 600
BssAI RCCGGY 2 cut(s) 227, 600
BssECI CCNNGG 2 cut(s) 423, 576
BssMI GATC 3 cut(s) 98, 522, 560
BssNI GRCGYC 4 cut(s) 301, 384, 420, 429
Bst2UI CCWGG 2 cut(s) 203, 298
Bst4CI ACNGT 2 cut(s) 412, 540
Bst6I CTCTTC 2 cut(s) 149, 341
BstACI GRCGYC 4 cut(s) 301, 384, 420, 429
BstC8I GCNNGC 3 cut(s) 189, 332, 567
BstDEI CTNAG 2 cut(s) 162, 501
BstF5I GGATG 1 cut(s) 387
BstFNI CGCG 4 cut(s) 97, 194, 258, 381
BstH2I RGCGCY 2 cut(s) 304, 423
BstHHI GCGC 4 cut(s) 260, 303, 422, 449
BstKTI GATC 3 cut(s) 101, 525, 563
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 3 cut(s) 98, 522, 560
BstMWI GCNNNNNNNGC 2 cut(s) 274, 331
BstNI CCWGG 2 cut(s) 203, 298
BstSCI CCNGG 2 cut(s) 201, 296
BstUI CGCG 4 cut(s) 97, 194, 258, 381
BstV2I GAAGAC 1 cut(s) 150
BsuRI GGCC 2 cut(s) 191, 201
BtgZI GCGATG 1 cut(s) 475
BtrI CACGTC 1 cut(s) 358
BtsCI GGATG 1 cut(s) 387
BveI ACCTGC 1 cut(s) 410
Cac8I GCNNGC 3 cut(s) 189, 332, 567
CfoI GCGC 4 cut(s) 260, 303, 422, 449
Cfr10I RCCGGY 2 cut(s) 227, 600
Cfr13I GGNCC 5 cut(s) 18, 189, 199, 205, 243
CseI GACGC 1 cut(s) 373
Csp6I GTAC 2 cut(s) 286, 484
CviAII CATG 1 cut(s) 481
CviJI RGCY 9 cut(s) 71, 166, 191, 201, 213, 268, 334, 442, 457
CviKI_1 RGCY 9 cut(s) 71, 166, 191, 201, 213, 268, 334, 442, 457
CviQI GTAC 2 cut(s) 286, 484
DdeI CTNAG 2 cut(s) 162, 501
DinI GGCGCC 2 cut(s) 302, 421
DpnI GATC 3 cut(s) 100, 524, 562
DpnII GATC 3 cut(s) 98, 522, 560
DrdI GACNNNNNNGTC 1 cut(s) 356
DseDI GACNNNNNNGTC 1 cut(s) 356
Eam1104I CTCTTC 2 cut(s) 149, 341
EarI CTCTTC 2 cut(s) 149, 341
Ecl136II GAGCTC 1 cut(s) 457
Eco24I GRGCYC 1 cut(s) 459
Eco47I GGWCC 3 cut(s) 18, 205, 243
Eco53kI GAGCTC 1 cut(s) 457
EcoICRI GAGCTC 1 cut(s) 457
EcoO109I RGGNCCY 1 cut(s) 205
EcoRI GAATTC 1 cut(s) 236
EcoRII CCWGG 2 cut(s) 201, 296
EcoT38I GRGCYC 1 cut(s) 459
EgeI GGCGCC 2 cut(s) 302, 421
EheI GGCGCC 2 cut(s) 302, 421
FaeI CATG 1 cut(s) 484
FaiI YATR 4 cut(s) 31, 61, 85, 482
FatI CATG 1 cut(s) 480
FauI CCCGC 1 cut(s) 180
Fnu4HI GCNGC 5 cut(s) 192, 261, 304, 307, 382
FokI GGATG 1 cut(s) 374
FriOI GRGCYC 1 cut(s) 459
Fsp4HI GCNGC 5 cut(s) 192, 261, 304, 307, 382
GlaI GCGC 4 cut(s) 259, 302, 421, 448
GluI GCNGC 5 cut(s) 192, 261, 304, 307, 382
GsaI CCCAGC 1 cut(s) 71
GsuI CTGGAG 2 cut(s) 19, 541
HaeII RGCGCY 2 cut(s) 304, 423
HaeIII GGCC 2 cut(s) 191, 201
HapII CCGG 2 cut(s) 228, 601
HgaI GACGC 1 cut(s) 373
HhaI GCGC 4 cut(s) 260, 303, 422, 449
Hin1I GRCGYC 4 cut(s) 301, 384, 420, 429
Hin1II CATG 1 cut(s) 484
Hin6I GCGC 4 cut(s) 258, 301, 420, 447
HinP1I GCGC 4 cut(s) 258, 301, 420, 447
HinfI GANTC 2 cut(s) 350, 572
HpaII CCGG 2 cut(s) 228, 601
HphI GGTGA 3 cut(s) 262, 352, 400
Hpy188I TCNGA 2 cut(s) 536, 577
Hpy188III TCNNGA 4 cut(s) 77, 169, 292, 558
Hpy99I CGWCG 3 cut(s) 199, 398, 587
HpyAV CCTTC 3 cut(s) 460, 499, 505
HpyCH4III ACNGT 2 cut(s) 412, 540
HpyCH4IV ACGT 2 cut(s) 357, 429
HpyCH4V TGCA 1 cut(s) 6
HpyF10VI GCNNNNNNNGC 2 cut(s) 274, 331
HpyF3I CTNAG 2 cut(s) 162, 501
HpySE526I ACGT 2 cut(s) 357, 429
Hsp92I GRCGYC 4 cut(s) 301, 384, 420, 429
Hsp92II CATG 1 cut(s) 484
HspAI GCGC 4 cut(s) 258, 301, 420, 447
KasI GGCGCC 2 cut(s) 300, 419
Kzo9I GATC 3 cut(s) 98, 522, 560
LguI GCTCTTC 1 cut(s) 341
LweI GCATC 1 cut(s) 240
MaeII ACGT 2 cut(s) 357, 429
MaeIII GTNAC 1 cut(s) 358
MalI GATC 3 cut(s) 100, 524, 562
MboI GATC 3 cut(s) 98, 522, 560
MboII GAAGA 3 cut(s) 136, 150, 328
MhlI GDGCHC 1 cut(s) 459
MluCI AATT 1 cut(s) 236
Mly113I GGCGCC 2 cut(s) 301, 420
MlyI GAGTC 1 cut(s) 344
MmeI TCCRAC 2 cut(s) 45, 270
MspA1I CMGCKG 1 cut(s) 309
MspI CCGG 2 cut(s) 228, 601
MspR9I CCNGG 2 cut(s) 203, 298
MvaI CCWGG 2 cut(s) 203, 298
MvnI CGCG 4 cut(s) 97, 194, 258, 381
MwoI GCNNNNNNNGC 2 cut(s) 274, 331
NarI GGCGCC 2 cut(s) 301, 420
NdeII GATC 3 cut(s) 98, 522, 560
NlaIII CATG 1 cut(s) 484
NlaIV GGNNCC 6 cut(s) 19, 65, 207, 302, 400, 421
NmeAIII GCCGAG 1 cut(s) 448
NmuCI GTSAC 1 cut(s) 358
PaqCI CACCTGC 1 cut(s) 410
PciSI GCTCTTC 1 cut(s) 341
PcsI WCGNNNNNNNCGW 2 cut(s) 573, 588
PfeI GAWTC 1 cut(s) 572
PkrI GCNGC 5 cut(s) 193, 262, 305, 308, 383
PleI GAGTC 1 cut(s) 344
PluTI GGCGCC 2 cut(s) 304, 423
PpsI GAGTC 1 cut(s) 344
PpuMI RGGWCCY 1 cut(s) 205
Psp124BI GAGCTC 1 cut(s) 459
Psp5II RGGWCCY 1 cut(s) 205
Psp6I CCWGG 2 cut(s) 201, 296
PspFI CCCAGC 1 cut(s) 67
PspGI CCWGG 2 cut(s) 201, 296
PspN4I GGNNCC 6 cut(s) 19, 65, 207, 302, 400, 421
PspPI GGNCC 5 cut(s) 18, 189, 199, 205, 243
PspPPI RGGWCCY 1 cut(s) 205
RsaI GTAC 2 cut(s) 287, 485
RsaNI GTAC 2 cut(s) 286, 484
SacI GAGCTC 1 cut(s) 459
SapI GCTCTTC 1 cut(s) 341
SatI GCNGC 5 cut(s) 192, 261, 304, 307, 382
Sau3AI GATC 3 cut(s) 98, 522, 560
Sau96I GGNCC 5 cut(s) 18, 189, 199, 205, 243
SchI GAGTC 1 cut(s) 344
ScrFI CCNGG 2 cut(s) 203, 298
SduI GDGCHC 1 cut(s) 459
SetI ASST 9 cut(s) 73, 168, 299, 336, 360, 404, 432, 459, 582
SfaNI GCATC 1 cut(s) 240
SfoI GGCGCC 2 cut(s) 302, 421
SgrAI CRCCGGYG 1 cut(s) 600
SinI GGWCC 3 cut(s) 18, 205, 243
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 1 cut(s) 236
SsiI CCGC 8 cut(s) 95, 187, 192, 261, 304, 307, 381, 598
SspDI GGCGCC 2 cut(s) 300, 419
SstI GAGCTC 1 cut(s) 459
StyD4I CCNGG 2 cut(s) 201, 296
TaaI ACNGT 2 cut(s) 412, 540
TaiI ACGT 2 cut(s) 360, 432
TaqI TCGA 3 cut(s) 234, 453, 585
TasI AATT 1 cut(s) 236
TatI WGTACW 2 cut(s) 285, 483
TauI GCSGC 5 cut(s) 194, 263, 306, 309, 384
TfiI GAWTC 1 cut(s) 572
TseFI GTSAC 1 cut(s) 358
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 1 cut(s) 478
TspGWI ACGGA 2 cut(s) 79, 144
VpaK11BI GGWCC 3 cut(s) 18, 205, 243
XapI RAATTY 1 cut(s) 236
ZraI GACGTC 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.