RLG00000036030

Pleckstrin homology domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0009844
Physical Location & Seq
Forward (+)
75109369 .. 75111086
1718 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036030

Sequence Viewer

Length: 660 bp
ATGTGCAAGATTGTGGGGTCCAACTTCTCATACTCCAGCACAGAGAACGACAGCGTATTATGGCACCCAGCTAATCACGAACCATATTCCGTTCCGCGATCCCAAAAAGGGGCAACTGAAACTCCCAAACCCCTCTACTCTCTCTCTCTCTTCCCATCTCCGCCTTCTCAGCTCATGATGGAGGGTCTGTGGCGGGCCGCGACGGGCCAGGACCCAAGCCCAGAAGACTACACCGGCATCGAATTCTGGTCCAACCCCGAACGCGCCGCCTGGCTCACCAAGCAAGGCGAGTACATCAAGACCTGGCGCCGCCGCTGGTTCGTCCTCAAGCAAGGCAAGCTCTTCTGGTTCAAGGACTCCCACGTCACCCCCTCTTCCACCCCACGCGGCGTCATCCCCGTCGGCACCTGCCTCACCGTCAAGGGCGCCGAGGACGTCCTCCACAAGCCCTGCGCCTTCGAGCTCTCCACCACCACCCACGACACCATCACTCCAGATCGCTCGCCGATTCCGAGGTCGTCGATTACGATAACCGCCGGTGATGCTTGTCCCGAAGTCCCTCATCTGAACTCATTTGATAATTTGTATGGCTGTTTGCACTTTCTGAGTTCTGTGGTCGGATCTTGTCAGATTTTCTTTCACAAGGATTTGCGGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PH

Protein Analysis

220

Amino Acids

24.56

Weight (kDa)

7.07

Isoelectric Point (pI)

55.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 90 - 161 1e-11 PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 416
AasI GACNNNNNNGTC 1 cut(s) 362
AatII GACGTC 1 cut(s) 438
Acc36I ACCTGC 1 cut(s) 416
AccB1I GGYRCC 4 cut(s) 63, 306, 404, 425
AccII CGCG 4 cut(s) 97, 200, 264, 387
AclWI GGATC 2 cut(s) 93, 628
AcsI RAATTY 1 cut(s) 242
AcyI GRCGYC 4 cut(s) 307, 390, 426, 435
AfaI GTAC 1 cut(s) 293
AfiI CCNNNNNNNGG 2 cut(s) 108, 109
AgsI TTSAA 1 cut(s) 352
AjiI CACGTC 1 cut(s) 364
AjnI CCWGG 3 cut(s) 207, 269, 302
AluBI AGCT 4 cut(s) 71, 172, 340, 463
AluI AGCT 4 cut(s) 71, 172, 340, 463
Alw21I GWGCWC 1 cut(s) 465
AlwI GGATC 2 cut(s) 93, 628
AoxI GGCC 2 cut(s) 195, 205
ApoI RAATTY 1 cut(s) 242
AspLEI GCGC 4 cut(s) 266, 309, 428, 455
AspS9I GGNCC 5 cut(s) 18, 195, 205, 211, 249
AsuHPI GGTGA 4 cut(s) 268, 358, 406, 551
AvaII GGWCC 3 cut(s) 18, 211, 249
BanI GGYRCC 4 cut(s) 63, 306, 404, 425
BanII GRGCYC 1 cut(s) 465
BbsI GAAGAC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 465
BccI CCATC 3 cut(s) 163, 172, 494
BciT130I CCWGG 3 cut(s) 209, 271, 304
BfoI RGCGCY 2 cut(s) 310, 429
BfuAI ACCTGC 1 cut(s) 416
BisI GCNGC 5 cut(s) 198, 267, 310, 313, 388
BlsI GCNGC 5 cut(s) 199, 268, 311, 314, 389
Bme1390I CCNGG 3 cut(s) 209, 271, 304
Bme18I GGWCC 3 cut(s) 18, 211, 249
BmgBI CACGTC 1 cut(s) 364
BmgT120I GGNCC 5 cut(s) 18, 195, 205, 211, 249
BmiI GGNNCC 6 cut(s) 19, 65, 213, 308, 406, 427
BmrFI CCNGG 3 cut(s) 209, 271, 304
BmsI GCATC 2 cut(s) 246, 532
BpiI GAAGAC 1 cut(s) 231
BpmI CTGGAG 2 cut(s) 19, 477
BpuEI CTTGAG 1 cut(s) 311
BsaHI GRCGYC 4 cut(s) 307, 390, 426, 435
BsaJI CCNNGG 2 cut(s) 429, 512
BsaXI ACNNNNNCTCC 6 cut(s) 106, 136, 173, 203, 475, 505
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 109
Bse118I RCCGGY 2 cut(s) 233, 536
BseBI CCWGG 3 cut(s) 209, 271, 304
BseDI CCNNGG 2 cut(s) 429, 512
BseGI GGATG 1 cut(s) 393
BseLI CCNNNNNNNGG 2 cut(s) 108, 109
BseMII CTCAG 2 cut(s) 182, 596
BseYI CCCAGC 1 cut(s) 67
Bsh1236I CGCG 4 cut(s) 97, 200, 264, 387
BshFI GGCC 2 cut(s) 197, 207
BshNI GGYRCC 4 cut(s) 63, 306, 404, 425
BsiHKAI GWGCWC 1 cut(s) 465
BsiSI CCGG 2 cut(s) 234, 537
BslFI GGGAC 2 cut(s) 534, 542
BslI CCNNNNNNNGG 2 cut(s) 108, 109
BsmFI GGGAC 2 cut(s) 534, 542
BsnI GGCC 2 cut(s) 197, 207
Bsp1286I GDGCHC 1 cut(s) 465
Bsp143I GATC 3 cut(s) 98, 496, 620
BspANI GGCC 2 cut(s) 197, 207
BspCNI CTCAG 2 cut(s) 181, 597
BspFNI CGCG 4 cut(s) 97, 200, 264, 387
BspHI TCATGA 1 cut(s) 174
BspLI GGNNCC 6 cut(s) 19, 65, 213, 308, 406, 427
BspMI ACCTGC 1 cut(s) 416
BspPI GGATC 2 cut(s) 93, 628
BspQI GCTCTTC 1 cut(s) 347
BspT107I GGYRCC 4 cut(s) 63, 306, 404, 425
BsrFI RCCGGY 2 cut(s) 233, 536
BssAI RCCGGY 2 cut(s) 233, 536
BssECI CCNNGG 2 cut(s) 429, 512
BssMI GATC 3 cut(s) 98, 496, 620
BssNI GRCGYC 4 cut(s) 307, 390, 426, 435
Bst2UI CCWGG 3 cut(s) 209, 271, 304
Bst4CI ACNGT 1 cut(s) 418
Bst6I CTCTTC 3 cut(s) 155, 347, 379
BstACI GRCGYC 4 cut(s) 307, 390, 426, 435
BstC8I GCNNGC 3 cut(s) 195, 338, 503
BstDEI CTNAG 2 cut(s) 168, 605
BstF5I GGATG 1 cut(s) 393
BstFNI CGCG 4 cut(s) 97, 200, 264, 387
BstH2I RGCGCY 2 cut(s) 310, 429
BstHHI GCGC 4 cut(s) 266, 309, 428, 455
BstKTI GATC 3 cut(s) 101, 499, 623
BstMBI GATC 3 cut(s) 98, 496, 620
BstMWI GCNNNNNNNGC 4 cut(s) 169, 280, 337, 542
BstNI CCWGG 3 cut(s) 209, 271, 304
BstSCI CCNGG 3 cut(s) 207, 269, 302
BstUI CGCG 4 cut(s) 97, 200, 264, 387
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 620
BstYI RGATCY 1 cut(s) 620
BsuRI GGCC 2 cut(s) 197, 207
BtrI CACGTC 1 cut(s) 364
BtsCI GGATG 1 cut(s) 393
BveI ACCTGC 1 cut(s) 416
Cac8I GCNNGC 3 cut(s) 195, 338, 503
CciI TCATGA 1 cut(s) 174
CfoI GCGC 4 cut(s) 266, 309, 428, 455
Cfr10I RCCGGY 2 cut(s) 233, 536
Cfr13I GGNCC 5 cut(s) 18, 195, 205, 211, 249
CseI GACGC 1 cut(s) 379
Csp6I GTAC 1 cut(s) 292
CviAII CATG 1 cut(s) 175
CviQI GTAC 1 cut(s) 292
DdeI CTNAG 2 cut(s) 168, 605
DinI GGCGCC 2 cut(s) 308, 427
DpnI GATC 3 cut(s) 100, 498, 622
DpnII GATC 3 cut(s) 98, 496, 620
DrdI GACNNNNNNGTC 1 cut(s) 362
DseDI GACNNNNNNGTC 1 cut(s) 362
Eam1104I CTCTTC 3 cut(s) 155, 347, 379
EarI CTCTTC 3 cut(s) 155, 347, 379
EciI GGCGGA 1 cut(s) 150
Ecl136II GAGCTC 1 cut(s) 463
Eco24I GRGCYC 1 cut(s) 465
Eco47I GGWCC 3 cut(s) 18, 211, 249
Eco53kI GAGCTC 1 cut(s) 463
EcoICRI GAGCTC 1 cut(s) 463
EcoO109I RGGNCCY 1 cut(s) 211
EcoRI GAATTC 1 cut(s) 242
EcoRII CCWGG 3 cut(s) 207, 269, 302
EcoT38I GRGCYC 1 cut(s) 465
EgeI GGCGCC 2 cut(s) 308, 427
EheI GGCGCC 2 cut(s) 308, 427
FaeI CATG 1 cut(s) 178
FaiI YATR 5 cut(s) 31, 61, 85, 176, 588
FaqI GGGAC 2 cut(s) 534, 542
FatI CATG 1 cut(s) 174
FauI CCCGC 2 cut(s) 186, 645
Fnu4HI GCNGC 5 cut(s) 198, 267, 310, 313, 388
FokI GGATG 1 cut(s) 380
FriOI GRGCYC 1 cut(s) 465
Fsp4HI GCNGC 5 cut(s) 198, 267, 310, 313, 388
GlaI GCGC 4 cut(s) 265, 308, 427, 454
GluI GCNGC 5 cut(s) 198, 267, 310, 313, 388
GsaI CCCAGC 1 cut(s) 71
GsuI CTGGAG 2 cut(s) 19, 477
HaeII RGCGCY 2 cut(s) 310, 429
HaeIII GGCC 2 cut(s) 197, 207
HapII CCGG 2 cut(s) 234, 537
HgaI GACGC 1 cut(s) 379
HhaI GCGC 4 cut(s) 266, 309, 428, 455
Hin1I GRCGYC 4 cut(s) 307, 390, 426, 435
Hin1II CATG 1 cut(s) 178
Hin6I GCGC 4 cut(s) 264, 307, 426, 453
HinP1I GCGC 4 cut(s) 264, 307, 426, 453
HinfI GANTC 2 cut(s) 356, 508
HpaII CCGG 2 cut(s) 234, 537
HphI GGTGA 4 cut(s) 268, 358, 406, 551
Hpy188I TCNGA 5 cut(s) 513, 567, 606, 620, 630
Hpy188III TCNNGA 5 cut(s) 77, 175, 298, 494, 551
Hpy99I CGWCG 3 cut(s) 205, 404, 523
HpyAV CCTTC 2 cut(s) 174, 466
HpyCH4III ACNGT 1 cut(s) 418
HpyCH4IV ACGT 2 cut(s) 363, 435
HpyCH4V TGCA 2 cut(s) 6, 598
HpyF10VI GCNNNNNNNGC 4 cut(s) 169, 280, 337, 542
HpyF3I CTNAG 2 cut(s) 168, 605
HpySE526I ACGT 2 cut(s) 363, 435
Hsp92I GRCGYC 4 cut(s) 307, 390, 426, 435
Hsp92II CATG 1 cut(s) 178
HspAI GCGC 4 cut(s) 264, 307, 426, 453
KasI GGCGCC 2 cut(s) 306, 425
Kzo9I GATC 3 cut(s) 98, 496, 620
LguI GCTCTTC 1 cut(s) 347
LweI GCATC 2 cut(s) 246, 532
MaeII ACGT 2 cut(s) 363, 435
MaeIII GTNAC 1 cut(s) 364
MalI GATC 3 cut(s) 100, 498, 622
MboI GATC 3 cut(s) 98, 496, 620
MboII GAAGA 4 cut(s) 142, 236, 334, 366
MflI RGATCY 1 cut(s) 620
MhlI GDGCHC 1 cut(s) 465
MluCI AATT 2 cut(s) 242, 580
Mly113I GGCGCC 2 cut(s) 307, 426
MlyI GAGTC 1 cut(s) 350
MmeI TCCRAC 3 cut(s) 45, 276, 598
MnlI CCTC 9 cut(s) 143, 175, 335, 382, 422, 424, 449, 507, 570
MspA1I CMGCKG 1 cut(s) 315
MspI CCGG 2 cut(s) 234, 537
MspR9I CCNGG 3 cut(s) 209, 271, 304
MvaI CCWGG 3 cut(s) 209, 271, 304
MvnI CGCG 4 cut(s) 97, 200, 264, 387
MwoI GCNNNNNNNGC 4 cut(s) 169, 280, 337, 542
NarI GGCGCC 2 cut(s) 307, 426
NdeII GATC 3 cut(s) 98, 496, 620
NlaIII CATG 1 cut(s) 178
NlaIV GGNNCC 6 cut(s) 19, 65, 213, 308, 406, 427
NmeAIII GCCGAG 1 cut(s) 454
NmuCI GTSAC 1 cut(s) 364
PagI TCATGA 1 cut(s) 174
PaqCI CACCTGC 1 cut(s) 416
PciSI GCTCTTC 1 cut(s) 347
PcsI WCGNNNNNNNCGW 2 cut(s) 509, 524
PfeI GAWTC 1 cut(s) 508
PkrI GCNGC 5 cut(s) 199, 268, 311, 314, 389
PleI GAGTC 1 cut(s) 350
PluTI GGCGCC 2 cut(s) 310, 429
PpsI GAGTC 1 cut(s) 350
PpuMI RGGWCCY 1 cut(s) 211
Psp124BI GAGCTC 1 cut(s) 465
Psp5II RGGWCCY 1 cut(s) 211
Psp6I CCWGG 3 cut(s) 207, 269, 302
PspFI CCCAGC 1 cut(s) 67
PspGI CCWGG 3 cut(s) 207, 269, 302
PspN4I GGNNCC 6 cut(s) 19, 65, 213, 308, 406, 427
PspPI GGNCC 5 cut(s) 18, 195, 205, 211, 249
PspPPI RGGWCCY 1 cut(s) 211
PsuI RGATCY 1 cut(s) 620
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
SacI GAGCTC 1 cut(s) 465
SapI GCTCTTC 1 cut(s) 347
SatI GCNGC 5 cut(s) 198, 267, 310, 313, 388
Sau3AI GATC 3 cut(s) 98, 496, 620
Sau96I GGNCC 5 cut(s) 18, 195, 205, 211, 249
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 3 cut(s) 209, 271, 304
SduI GDGCHC 1 cut(s) 465
SetI ASST 9 cut(s) 73, 174, 305, 342, 366, 410, 438, 465, 518
SfaNI GCATC 2 cut(s) 246, 532
SfoI GGCGCC 2 cut(s) 308, 427
SgrAI CRCCGGYG 1 cut(s) 536
SinI GGWCC 3 cut(s) 18, 211, 249
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
Sse9I AATT 2 cut(s) 242, 580
SspDI GGCGCC 2 cut(s) 306, 425
SstI GAGCTC 1 cut(s) 465
StyD4I CCNGG 3 cut(s) 207, 269, 302
TaaI ACNGT 1 cut(s) 418
TaiI ACGT 2 cut(s) 366, 438
TaqI TCGA 3 cut(s) 240, 459, 521
TasI AATT 2 cut(s) 242, 580
TatI WGTACW 1 cut(s) 291
TauI GCSGC 5 cut(s) 200, 269, 312, 315, 390
TfiI GAWTC 1 cut(s) 508
TseFI GTSAC 1 cut(s) 364
Tsp45I GTSAC 1 cut(s) 364
TspGWI ACGGA 1 cut(s) 79
VpaK11BI GGWCC 3 cut(s) 18, 211, 249
XapI RAATTY 1 cut(s) 242
ZraI GACGTC 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.