Rw0G006110

Pleckstrin homology domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00235
OG0009844
Physical Location & Seq
Reverse (-)
157620 .. 158048
429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G006110.1

Sequence Viewer

Length: 429 bp
ATGGAGGGTCTGTGGCGGGCCGCGACGGGCCAGGACCCAAGCCCAGAAGACTACACCGGCATCGAATTCTGGTCCAACCCCGAACGCGCCGCCTGGCTCACCAAGCAAGGCGAGTACATCAAGACCTGGCGCCGCCGCTGGTTCGTCCTCAAGCAAGGCAAGCTCTTCTGGTTCAAGGACTCCCACGTCACCCCCTCCTCCACCCCACGCGGCGTCATCCCCGTCGGCACCTGCCTCACCGTCAAGGGCGCCGAGGACGTCCTCCACAAGCCCTGCGCCTTCGAGCTCTCCACCACCACCCACGACACCATGTACTTCATCGCCGATACTGAGAAGGAGAAGGAGGAGTGGATCAACTCCATCGGACGGTCGTTAGTCCAGCCTCGAAGGAGAAGGAGGAGTGGATCAACTCCATCGGACGGTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PH

Protein Analysis

142

Amino Acids

16.38

Weight (kDa)

9.23

Isoelectric Point (pI)

63.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 30 - 123 1.2e-19 PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 239
AasI GACNNNNNNGTC 1 cut(s) 185
AatII GACGTC 1 cut(s) 261
Acc36I ACCTGC 1 cut(s) 239
AccB1I GGYRCC 3 cut(s) 129, 227, 248
AccII CGCG 3 cut(s) 23, 87, 210
AciI CCGC 6 cut(s) 16, 21, 90, 133, 136, 210
AclWI GGATC 2 cut(s) 359, 412
AcsI RAATTY 1 cut(s) 65
AcyI GRCGYC 4 cut(s) 130, 213, 249, 258
AfaI GTAC 2 cut(s) 116, 314
AfiI CCNNNNNNNGG 2 cut(s) 366, 419
AgsI TTSAA 1 cut(s) 175
AjiI CACGTC 1 cut(s) 187
AjnI CCWGG 3 cut(s) 30, 92, 125
AluBI AGCT 2 cut(s) 163, 286
AluI AGCT 2 cut(s) 163, 286
Alw21I GWGCWC 1 cut(s) 288
AlwI GGATC 2 cut(s) 359, 412
AoxI GGCC 2 cut(s) 18, 28
ApoI RAATTY 1 cut(s) 65
AspLEI GCGC 4 cut(s) 89, 132, 251, 278
AspS9I GGNCC 4 cut(s) 18, 28, 34, 72
AsuHPI GGTGA 3 cut(s) 91, 181, 229
AvaII GGWCC 2 cut(s) 34, 72
BanI GGYRCC 3 cut(s) 129, 227, 248
BanII GRGCYC 1 cut(s) 288
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 288
BccI CCATC 2 cut(s) 368, 421
BciT130I CCWGG 3 cut(s) 32, 94, 127
BfoI RGCGCY 2 cut(s) 133, 252
BfuAI ACCTGC 1 cut(s) 239
BisI GCNGC 5 cut(s) 21, 90, 133, 136, 211
BlsI GCNGC 5 cut(s) 22, 91, 134, 137, 212
Bme1390I CCNGG 3 cut(s) 32, 94, 127
Bme18I GGWCC 2 cut(s) 34, 72
BmgBI CACGTC 1 cut(s) 187
BmgT120I GGNCC 4 cut(s) 18, 28, 34, 72
BmiI GGNNCC 4 cut(s) 36, 131, 229, 250
BmrFI CCNGG 3 cut(s) 32, 94, 127
BmsI GCATC 1 cut(s) 69
BpiI GAAGAC 1 cut(s) 54
BpuEI CTTGAG 1 cut(s) 134
BsaHI GRCGYC 4 cut(s) 130, 213, 249, 258
BsaJI CCNNGG 1 cut(s) 252
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 366, 419
Bse118I RCCGGY 1 cut(s) 56
BseBI CCWGG 3 cut(s) 32, 94, 127
BseDI CCNNGG 1 cut(s) 252
BseGI GGATG 1 cut(s) 216
BseLI CCNNNNNNNGG 2 cut(s) 366, 419
BseMII CTCAG 1 cut(s) 321
BseRI GAGGAG 3 cut(s) 187, 359, 412
Bsh1236I CGCG 3 cut(s) 23, 87, 210
Bsh1285I CGRYCG 2 cut(s) 371, 424
BshFI GGCC 2 cut(s) 20, 30
BshNI GGYRCC 3 cut(s) 129, 227, 248
BsiEI CGRYCG 2 cut(s) 371, 424
BsiHKAI GWGCWC 1 cut(s) 288
BsiSI CCGG 1 cut(s) 57
BslI CCNNNNNNNGG 2 cut(s) 366, 419
BsnI GGCC 2 cut(s) 20, 30
Bsp1286I GDGCHC 1 cut(s) 288
Bsp143I GATC 2 cut(s) 351, 404
BspACI CCGC 6 cut(s) 16, 21, 90, 133, 136, 210
BspANI GGCC 2 cut(s) 20, 30
BspCNI CTCAG 1 cut(s) 322
BspFNI CGCG 3 cut(s) 23, 87, 210
BspLI GGNNCC 4 cut(s) 36, 131, 229, 250
BspMI ACCTGC 1 cut(s) 239
BspPI GGATC 2 cut(s) 359, 412
BspQI GCTCTTC 1 cut(s) 170
BspT107I GGYRCC 3 cut(s) 129, 227, 248
BsrFI RCCGGY 1 cut(s) 56
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 2 cut(s) 351, 404
BssNI GRCGYC 4 cut(s) 130, 213, 249, 258
Bst2UI CCWGG 3 cut(s) 32, 94, 127
Bst4CI ACNGT 3 cut(s) 241, 369, 422
Bst6I CTCTTC 1 cut(s) 170
BstACI GRCGYC 4 cut(s) 130, 213, 249, 258
BstC8I GCNNGC 2 cut(s) 18, 161
BstDEI CTNAG 1 cut(s) 330
BstF5I GGATG 1 cut(s) 216
BstFNI CGCG 3 cut(s) 23, 87, 210
BstH2I RGCGCY 2 cut(s) 133, 252
BstHHI GCGC 4 cut(s) 89, 132, 251, 278
BstKTI GATC 2 cut(s) 354, 407
BstMBI GATC 2 cut(s) 351, 404
BstMCI CGRYCG 2 cut(s) 371, 424
BstMWI GCNNNNNNNGC 2 cut(s) 103, 160
BstNI CCWGG 3 cut(s) 32, 94, 127
BstSCI CCNGG 3 cut(s) 30, 92, 125
BstUI CGCG 3 cut(s) 23, 87, 210
BstV2I GAAGAC 1 cut(s) 54
BsuRI GGCC 2 cut(s) 20, 30
BtgZI GCGATG 1 cut(s) 304
BtrI CACGTC 1 cut(s) 187
BtsCI GGATG 1 cut(s) 216
BveI ACCTGC 1 cut(s) 239
Cac8I GCNNGC 2 cut(s) 18, 161
CfoI GCGC 4 cut(s) 89, 132, 251, 278
Cfr10I RCCGGY 1 cut(s) 56
Cfr13I GGNCC 4 cut(s) 18, 28, 34, 72
CseI GACGC 1 cut(s) 202
Csp6I GTAC 2 cut(s) 115, 313
CviAII CATG 1 cut(s) 310
CviJI RGCY 8 cut(s) 20, 30, 42, 97, 163, 271, 286, 382
CviKI_1 RGCY 8 cut(s) 20, 30, 42, 97, 163, 271, 286, 382
CviQI GTAC 2 cut(s) 115, 313
DdeI CTNAG 1 cut(s) 330
DinI GGCGCC 2 cut(s) 131, 250
DpnI GATC 2 cut(s) 353, 406
DpnII GATC 2 cut(s) 351, 404
DrdI GACNNNNNNGTC 1 cut(s) 185
DseDI GACNNNNNNGTC 1 cut(s) 185
Eam1104I CTCTTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 170
Ecl136II GAGCTC 1 cut(s) 286
Eco24I GRGCYC 1 cut(s) 288
Eco47I GGWCC 2 cut(s) 34, 72
Eco53kI GAGCTC 1 cut(s) 286
EcoICRI GAGCTC 1 cut(s) 286
EcoO109I RGGNCCY 1 cut(s) 34
EcoRI GAATTC 1 cut(s) 65
EcoRII CCWGG 3 cut(s) 30, 92, 125
EcoT38I GRGCYC 1 cut(s) 288
EgeI GGCGCC 2 cut(s) 131, 250
EheI GGCGCC 2 cut(s) 131, 250
FaeI CATG 1 cut(s) 313
FaiI YATR 1 cut(s) 311
FatI CATG 1 cut(s) 309
FauI CCCGC 1 cut(s) 9
Fnu4HI GCNGC 5 cut(s) 21, 90, 133, 136, 211
FokI GGATG 1 cut(s) 203
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 5 cut(s) 21, 90, 133, 136, 211
GlaI GCGC 4 cut(s) 88, 131, 250, 277
GluI GCNGC 5 cut(s) 21, 90, 133, 136, 211
HaeII RGCGCY 2 cut(s) 133, 252
HaeIII GGCC 2 cut(s) 20, 30
HapII CCGG 1 cut(s) 57
HgaI GACGC 1 cut(s) 202
HhaI GCGC 4 cut(s) 89, 132, 251, 278
Hin1I GRCGYC 4 cut(s) 130, 213, 249, 258
Hin1II CATG 1 cut(s) 313
Hin6I GCGC 4 cut(s) 87, 130, 249, 276
HinP1I GCGC 4 cut(s) 87, 130, 249, 276
HinfI GANTC 1 cut(s) 179
HpaII CCGG 1 cut(s) 57
HphI GGTGA 3 cut(s) 91, 181, 229
Hpy188I TCNGA 2 cut(s) 365, 418
Hpy188III TCNNGA 1 cut(s) 121
Hpy99I CGWCG 2 cut(s) 28, 227
HpyAV CCTTC 5 cut(s) 289, 328, 334, 381, 387
HpyCH4III ACNGT 3 cut(s) 241, 369, 422
HpyCH4IV ACGT 2 cut(s) 186, 258
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 160
HpyF3I CTNAG 1 cut(s) 330
HpySE526I ACGT 2 cut(s) 186, 258
Hsp92I GRCGYC 4 cut(s) 130, 213, 249, 258
Hsp92II CATG 1 cut(s) 313
HspAI GCGC 4 cut(s) 87, 130, 249, 276
KasI GGCGCC 2 cut(s) 129, 248
Kzo9I GATC 2 cut(s) 351, 404
LguI GCTCTTC 1 cut(s) 170
LweI GCATC 1 cut(s) 69
MaeII ACGT 2 cut(s) 186, 258
MaeIII GTNAC 1 cut(s) 187
MalI GATC 2 cut(s) 353, 406
MboI GATC 2 cut(s) 351, 404
MboII GAAGA 2 cut(s) 59, 157
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 1 cut(s) 65
Mly113I GGCGCC 2 cut(s) 130, 249
MlyI GAGTC 1 cut(s) 173
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 9 cut(s) 158, 205, 208, 245, 247, 272, 337, 390, 393
MspA1I CMGCKG 1 cut(s) 138
MspI CCGG 1 cut(s) 57
MspR9I CCNGG 3 cut(s) 32, 94, 127
MvaI CCWGG 3 cut(s) 32, 94, 127
MvnI CGCG 3 cut(s) 23, 87, 210
MwoI GCNNNNNNNGC 2 cut(s) 103, 160
NarI GGCGCC 2 cut(s) 130, 249
NdeII GATC 2 cut(s) 351, 404
NlaIII CATG 1 cut(s) 313
NlaIV GGNNCC 4 cut(s) 36, 131, 229, 250
NmeAIII GCCGAG 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 187
PaqCI CACCTGC 1 cut(s) 239
PciSI GCTCTTC 1 cut(s) 170
PkrI GCNGC 5 cut(s) 22, 91, 134, 137, 212
PleI GAGTC 1 cut(s) 173
PluTI GGCGCC 2 cut(s) 133, 252
PpsI GAGTC 1 cut(s) 173
PpuMI RGGWCCY 1 cut(s) 34
Psp124BI GAGCTC 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 34
Psp6I CCWGG 3 cut(s) 30, 92, 125
PspGI CCWGG 3 cut(s) 30, 92, 125
PspN4I GGNNCC 4 cut(s) 36, 131, 229, 250
PspPI GGNCC 4 cut(s) 18, 28, 34, 72
PspPPI RGGWCCY 1 cut(s) 34
RsaI GTAC 2 cut(s) 116, 314
RsaNI GTAC 2 cut(s) 115, 313
SacI GAGCTC 1 cut(s) 288
SapI GCTCTTC 1 cut(s) 170
SatI GCNGC 5 cut(s) 21, 90, 133, 136, 211
Sau3AI GATC 2 cut(s) 351, 404
Sau96I GGNCC 4 cut(s) 18, 28, 34, 72
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 3 cut(s) 32, 94, 127
SduI GDGCHC 1 cut(s) 288
SetI ASST 6 cut(s) 128, 165, 189, 233, 261, 288
SfaNI GCATC 1 cut(s) 69
SfoI GGCGCC 2 cut(s) 131, 250
SinI GGWCC 2 cut(s) 34, 72
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 1 cut(s) 65
SsiI CCGC 6 cut(s) 16, 21, 90, 133, 136, 210
SspDI GGCGCC 2 cut(s) 129, 248
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 3 cut(s) 30, 92, 125
TaaI ACNGT 3 cut(s) 241, 369, 422
TaiI ACGT 2 cut(s) 189, 261
TaqI TCGA 3 cut(s) 63, 282, 385
TasI AATT 1 cut(s) 65
TatI WGTACW 2 cut(s) 114, 312
TauI GCSGC 5 cut(s) 23, 92, 135, 138, 213
TseFI GTSAC 1 cut(s) 187
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 1 cut(s) 307
VpaK11BI GGWCC 2 cut(s) 34, 72
XapI RAATTY 1 cut(s) 65
ZraI GACGTC 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.