Rorug05G0395600

Pleckstrin homology domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001422
Physical Location & Seq
Forward (+)
54249631 .. 54254988
5358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0395600.1

Sequence Viewer

Length: 1710 bp
ATGGTCCCTGGCATTGTAACCGGTGGCCTAGAGCTCTGGGAGGGCAGGCCTTGTGCTGAAGGTCTTTTTAGGAAGCGACTTTGGGGTGGTAGTTTCTCAGAGATCTTCAAAAGGCCTATGTGTTTGTTGGAGCACCTATCACTGGACAATGAAACAGGGCTGGACCCTCCAGGAATTCGAGTCAGAGCAGTTCGGGGACTCGCTGCAGCTGACTATTTTGCTCCTGGCTACTTTGTTTGGGCTGTCCTCATTAAGAATTTGGCAAAAATTGGTTATGAAGGGAAGAACATGCATACGGCTGCCTATGATTGGAGGCTTTCTTTCCAGAACACTGAGATTCGGGACCAAGCTCTTAGTAGGTTGAAGAGTAAAATTGAGCTTATGTATGCAATCAATGGCCATAAGAAAGTGGTTGTGGTGCCTCATTCTATGGGGGCTATTTATTTTCTCCACTTCATGAAATGGGTCGAGTCACCTCCTCCTATGGGAGGTGGTGGTGGTCCAAGTTGGTGTGCCACGCACATCAAAGCAATCATGAATATCGGTCCAGCATTTCTTGGTGTTCCAAGAGCAGTTAGTAATATATTCTCTGCTGAGGGTAAAGATGTTGCTTACATCAGAGCTATGGCTCCTGTTGTTTTAGATTCTGAAATTCTTGGGCTTCAAACCTTTGAACATGTCATGCGGGTATCTCGGAGCTGGGATTCCTTGATTTCCTTGTTGCCAAAAGGTGGAGAAACCATTTGGGGCAACTTGGAGTGGTCTGCTGAAGACGGGCATAGCTGTGACCTGACAAAGAAGAAATATCTGCATTCCACTGCTGGTGATACCAATTACAACAACAGCAATAAGAATATAATTTTCCAAGTAAAAGAACCTATGAAGTATGGAAGAATAATCTCATTTGGCAAGGAATCATCAGAATTACCCTCTTCAGAGCTTCCTGGTCATGATATAAAGGAACTTCTTTACAGGGATTCTCCTACCAATTTCAACTCATCATGTGGAGAGGTGTGGACTGAATATGATGAGGTAAGCAGGGAAAGCATAAGAAAAATTGCAGAAAATAAGGCTTACACAGTTCAAACCCTTTTTGATCTACTACGCTTTGTGGCACCAAAAATGATGCAACGGGCTGAGGCTCACTTCTCTCATGGGATAGCTGATAATCTTGATAATCCTAAATATGACCATTACAAGTACTGGTCCAATCCACTCGAGACCAAGTTACCCATTGCTCCAGATTTGGAAATATACTGTTTATATGGTGTTGGACTCCCAACTGAAAGATCATACATATACAAGACATCTCCTTCTGACAAGTGCAAGAACATCCCATTCCGCATTGATAGCTCAGCAGATGGAGAGACAGGCAGCTGCCTTAAAAATGGAGCACATTTTGTTGATGGTGATGATACCGTGCCTGTATTGAGTGCTGGATACATGTGCACCAAAGGGTGGAGAGGAAGAACCAGGTTCAACCCATCCGGTATTGCTACCTACATAAGGGAGTACCAGCACAAGCGACCTGGTAGTTTTATTGAGGGAAGGGGTACGGAGAGTGGTTCACATGTTGACATCATGGGAAATGTGGCATTAATCGAAGATATCCTATGCATTGCTGCCGGAGCCACTGGTGCACAGATTGGAGGTGATAGGATCTACTCAGATATTATGAGAATGTCTGAGAGAATAAATCTTCAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PH

Protein Analysis

569

Amino Acids

63.44

Weight (kDa)

6.97

Isoelectric Point (pI)

43.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 23 - 529 8.1e-66 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 418, 1114
AccB7I CCANNNNNTGG 2 cut(s) 731, 1458
AciI CCGC 2 cut(s) 685, 1342
AclWI GGATC 1 cut(s) 1667
AcoI YGGCCR 1 cut(s) 397
AcsI RAATTY 3 cut(s) 174, 256, 651
AcuI CTGAAG 4 cut(s) 78, 789, 918, 1685
AfaI GTAC 3 cut(s) 1202, 1514, 1555
AfiI CCNNNNNNNGG 7 cut(s) 142, 309, 485, 488, 731, 1458, 1506
AflIII ACRYGT 3 cut(s) 676, 1443, 1570
AgeI ACCGGT 1 cut(s) 20
AgsI TTSAA 7 cut(s) 109, 364, 665, 674, 994, 1085, 1480
AjnI CCWGG 6 cut(s) 7, 169, 223, 943, 1472, 1528
Alw21I GWGCWC 5 cut(s) 36, 135, 1396, 1451, 1642
Alw26I GTCTC 2 cut(s) 1214, 1361
Alw44I GTGCAC 2 cut(s) 1447, 1638
AlwI GGATC 1 cut(s) 1667
Ama87I CYCGRG 1 cut(s) 1217
AoxI GGCC 4 cut(s) 25, 47, 113, 397
ApaLI GTGCAC 2 cut(s) 1447, 1638
ApeKI GCWGC 6 cut(s) 203, 206, 299, 1374, 1377, 1622
ApoI RAATTY 3 cut(s) 174, 256, 651
AseI ATTAAT 1 cut(s) 1598
AsiGI ACCGGT 1 cut(s) 20
AspS9I GGNCC 6 cut(s) 4, 163, 343, 500, 545, 1206
AsuHPI GGTGA 4 cut(s) 465, 836, 1421, 1664
AvaI CYCGRG 1 cut(s) 1217
AvaII GGWCC 6 cut(s) 4, 163, 343, 500, 545, 1206
BaeGI GKGCMC 2 cut(s) 1451, 1642
BalI TGGCCA 1 cut(s) 399
BanI GGYRCC 2 cut(s) 418, 1114
BanII GRGCYC 1 cut(s) 36
BbsI GAAGAC 1 cut(s) 777
Bbv12I GWGCWC 5 cut(s) 36, 135, 1396, 1451, 1642
BbvCI CCTCAGC 2 cut(s) 594, 1137
BbvI GCAGC 6 cut(s) 190, 218, 286, 1364, 1386, 1609
BccI CCATC 3 cut(s) 1355, 1400, 1492
BceAI ACGGC 1 cut(s) 312
BciT130I CCWGG 6 cut(s) 9, 171, 225, 945, 1474, 1530
BciVI GTATCC 1 cut(s) 1433
BcoDI GTCTC 2 cut(s) 1214, 1361
BfaI CTAG 1 cut(s) 29
BfmI CTRYAG 1 cut(s) 204
BfuI GTATCC 1 cut(s) 1433
BglII AGATCT 1 cut(s) 102
BisI GCNGC 6 cut(s) 204, 207, 300, 1375, 1378, 1623
BlpI GCTNAGC 1 cut(s) 1354
BlsI GCNGC 6 cut(s) 205, 208, 301, 1376, 1379, 1624
BmcAI AGTACT 1 cut(s) 1202
Bme1390I CCNGG 6 cut(s) 9, 171, 225, 945, 1474, 1530
Bme18I GGWCC 6 cut(s) 4, 163, 343, 500, 545, 1206
BmeT110I CYCGRG 1 cut(s) 1217
BmgT120I GGNCC 6 cut(s) 4, 163, 343, 500, 545, 1206
BmiI GGNNCC 7 cut(s) 6, 165, 344, 420, 630, 1116, 1630
BmrFI CCNGG 6 cut(s) 9, 171, 225, 945, 1474, 1530
BmsI GCATC 1 cut(s) 1116
BpiI GAAGAC 1 cut(s) 777
BpmI CTGGAG 2 cut(s) 153, 1224
Bpu10I CCTNAGC 2 cut(s) 594, 1137
Bpu1102I GCTNAGC 1 cut(s) 1354
BsaI GGTCTC 1 cut(s) 1214
BsaJI CCNNGG 1 cut(s) 7
BsaWI WCCGGW 2 cut(s) 20, 1487
Bsc4I CCNNNNNNNGG 7 cut(s) 142, 309, 485, 488, 731, 1458, 1506
Bse118I RCCGGY 1 cut(s) 20
Bse1I ACTGG 3 cut(s) 147, 1208, 1639
Bse3DI GCAATG 2 cut(s) 1233, 1617
BseBI CCWGG 6 cut(s) 9, 171, 225, 945, 1474, 1530
BseDI CCNNGG 1 cut(s) 7
BseGI GGATG 2 cut(s) 1332, 1484
BseLI CCNNNNNNNGG 7 cut(s) 142, 309, 485, 488, 731, 1458, 1506
BseMI GCAATG 2 cut(s) 1233, 1617
BseMII CTCAG 7 cut(s) 111, 324, 585, 1128, 1368, 1677, 1680
BseNI ACTGG 3 cut(s) 147, 1208, 1639
BseRI GAGGAG 1 cut(s) 468
BseSI GKGCMC 2 cut(s) 1451, 1642
BseXI GCAGC 6 cut(s) 190, 218, 286, 1364, 1386, 1609
BseYI CCCAGC 1 cut(s) 699
BshFI GGCC 4 cut(s) 27, 49, 115, 399
BshNI GGYRCC 2 cut(s) 418, 1114
BshTI ACCGGT 1 cut(s) 20
BsiHKAI GWGCWC 5 cut(s) 36, 135, 1396, 1451, 1642
BsiHKCI CYCGRG 1 cut(s) 1217
BsiSI CCGG 3 cut(s) 21, 1488, 1626
BslFI GGGAC 2 cut(s) 210, 356
BslI CCNNNNNNNGG 7 cut(s) 142, 309, 485, 488, 731, 1458, 1506
BsmAI GTCTC 2 cut(s) 1214, 1361
BsmFI GGGAC 2 cut(s) 210, 356
BsmI GAATGC 1 cut(s) 811
BsnI GGCC 4 cut(s) 27, 49, 115, 399
Bso31I GGTCTC 1 cut(s) 1214
BsoBI CYCGRG 1 cut(s) 1217
Bsp1286I GDGCHC 5 cut(s) 36, 135, 1396, 1451, 1642
Bsp143I GATC 4 cut(s) 102, 1096, 1289, 1659
Bsp1720I GCTNAGC 1 cut(s) 1354
BspACI CCGC 2 cut(s) 685, 1342
BspANI GGCC 4 cut(s) 27, 49, 115, 399
BspCNI CTCAG 7 cut(s) 110, 325, 586, 1129, 1367, 1678, 1679
BspHI TCATGA 3 cut(s) 456, 534, 949
BspLI GGNNCC 7 cut(s) 6, 165, 344, 420, 630, 1116, 1630
BspMAI CTGCAG 1 cut(s) 208
BspPI GGATC 1 cut(s) 1667
BspT107I GGYRCC 2 cut(s) 418, 1114
BspTNI GGTCTC 1 cut(s) 1214
BsrDI GCAATG 2 cut(s) 1233, 1617
BsrFI RCCGGY 1 cut(s) 20
BsrI ACTGG 3 cut(s) 147, 1208, 1639
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 1 cut(s) 7
BssMI GATC 4 cut(s) 102, 1096, 1289, 1659
Bst2UI CCWGG 6 cut(s) 9, 171, 225, 945, 1474, 1530
Bst4CI ACNGT 3 cut(s) 1081, 1259, 1420
Bst6I CTCTTC 2 cut(s) 359, 937
BstC8I GCNNGC 1 cut(s) 47
BstDEI CTNAG 8 cut(s) 97, 333, 353, 594, 1137, 1354, 1666, 1686
BstENI CCTNNNNNAGG 2 cut(s) 486, 1504
BstF5I GGATG 2 cut(s) 1332, 1484
BstKTI GATC 4 cut(s) 105, 1099, 1292, 1662
BstMAI GTCTC 2 cut(s) 1214, 1361
BstMBI GATC 4 cut(s) 102, 1096, 1289, 1659
BstMWI GCNNNNNNNGC 4 cut(s) 1044, 1350, 1628, 1637
BstNI CCWGG 6 cut(s) 9, 171, 225, 945, 1474, 1530
BstNSI RCATGY 4 cut(s) 292, 680, 1447, 1574
BstSCI CCNGG 6 cut(s) 7, 169, 223, 943, 1472, 1528
BstSFI CTRYAG 1 cut(s) 204
BstSLI GKGCMC 2 cut(s) 1451, 1642
BstV1I GCAGC 6 cut(s) 190, 218, 286, 1364, 1386, 1609
BstV2I GAAGAC 1 cut(s) 777
BstX2I RGATCY 2 cut(s) 102, 1659
BstYI RGATCY 2 cut(s) 102, 1659
BsuI GTATCC 1 cut(s) 1433
BsuRI GGCC 4 cut(s) 27, 49, 115, 399
BtsCI GGATG 2 cut(s) 1332, 1484
BtsI GCAGTG 1 cut(s) 816
BtsIMutI CAGTG 4 cut(s) 140, 330, 816, 1632
Cac8I GCNNGC 1 cut(s) 47
CciI TCATGA 3 cut(s) 456, 534, 949
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 6 cut(s) 4, 163, 343, 500, 545, 1206
CsiI ACCWGGT 2 cut(s) 1472, 1528
Csp6I GTAC 3 cut(s) 1201, 1513, 1554
CspAI ACCGGT 1 cut(s) 20
CviQI GTAC 3 cut(s) 1201, 1513, 1554
DdeI CTNAG 8 cut(s) 97, 333, 353, 594, 1137, 1354, 1666, 1686
DpnI GATC 4 cut(s) 104, 1098, 1291, 1661
DpnII GATC 4 cut(s) 102, 1096, 1289, 1659
EaeI YGGCCR 1 cut(s) 397
Eam1104I CTCTTC 2 cut(s) 359, 937
EarI CTCTTC 2 cut(s) 359, 937
Ecl136II GAGCTC 1 cut(s) 34
Eco147I AGGCCT 2 cut(s) 49, 115
Eco24I GRGCYC 1 cut(s) 36
Eco31I GGTCTC 1 cut(s) 1214
Eco32I GATATC 1 cut(s) 1609
Eco47I GGWCC 6 cut(s) 4, 163, 343, 500, 545, 1206
Eco53kI GAGCTC 1 cut(s) 34
Eco57I CTGAAG 4 cut(s) 78, 789, 918, 1685
Eco88I CYCGRG 1 cut(s) 1217
EcoICRI GAGCTC 1 cut(s) 34
EcoNI CCTNNNNNAGG 2 cut(s) 486, 1504
EcoRI GAATTC 1 cut(s) 174
EcoRII CCWGG 6 cut(s) 7, 169, 223, 943, 1472, 1528
EcoRV GATATC 1 cut(s) 1609
EcoT22I ATGCAT 2 cut(s) 294, 1619
EcoT38I GRGCYC 1 cut(s) 36
FaqI GGGAC 2 cut(s) 210, 356
FauI CCCGC 1 cut(s) 678
Fnu4HI GCNGC 6 cut(s) 204, 207, 300, 1375, 1378, 1623
FokI GGATG 2 cut(s) 1319, 1471
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 6 cut(s) 204, 207, 300, 1375, 1378, 1623
FspBI CTAG 1 cut(s) 29
GluI GCNGC 6 cut(s) 204, 207, 300, 1375, 1378, 1623
GsaI CCCAGC 1 cut(s) 703
GsuI CTGGAG 2 cut(s) 153, 1224
HaeIII GGCC 4 cut(s) 27, 49, 115, 399
HapII CCGG 3 cut(s) 21, 1488, 1626
HincII GTYRAC 1 cut(s) 1576
HindII GTYRAC 1 cut(s) 1576
HinfI GANTC 9 cut(s) 180, 198, 337, 470, 644, 704, 914, 977, 1275
HpaII CCGG 3 cut(s) 21, 1488, 1626
HphI GGTGA 4 cut(s) 465, 836, 1421, 1664
Hpy166II GTNNAC 5 cut(s) 1017, 1449, 1568, 1576, 1640
Hpy188III TCNNGA 8 cut(s) 325, 341, 457, 535, 950, 1172, 1219, 1241
Hpy8I GTNNAC 5 cut(s) 1017, 1449, 1568, 1576, 1640
HpyAV CCTTC 4 cut(s) 53, 272, 1323, 1542
HpyCH4III ACNGT 3 cut(s) 1081, 1259, 1420
HpyF10VI GCNNNNNNNGC 4 cut(s) 1044, 1350, 1628, 1637
HpyF3I CTNAG 8 cut(s) 97, 333, 353, 594, 1137, 1354, 1666, 1686
Kzo9I GATC 4 cut(s) 102, 1096, 1289, 1659
LmnI GCTCC 7 cut(s) 130, 226, 634, 696, 1243, 1391, 1628
Lsp1109I GCAGC 6 cut(s) 190, 218, 286, 1364, 1386, 1609
LweI GCATC 1 cut(s) 1116
MabI ACCWGGT 2 cut(s) 1472, 1528
MaeI CTAG 1 cut(s) 29
MaeIII GTNAC 4 cut(s) 16, 471, 785, 1227
MalI GATC 4 cut(s) 104, 1098, 1291, 1661
MboI GATC 4 cut(s) 102, 1096, 1289, 1659
MflI RGATCY 2 cut(s) 102, 1659
MhlI GDGCHC 5 cut(s) 36, 135, 1396, 1451, 1642
MlsI TGGCCA 1 cut(s) 399
MluNI TGGCCA 1 cut(s) 399
MlyI GAGTC 4 cut(s) 189, 192, 479, 1269
MmeI TCCRAC 2 cut(s) 108, 1252
Mox20I TGGCCA 1 cut(s) 399
Mph1103I ATGCAT 2 cut(s) 294, 1619
MscI TGGCCA 1 cut(s) 399
MseI TTAA 3 cut(s) 252, 1383, 1598
MslI CAYNNNNRTG 1 cut(s) 783
Msp20I TGGCCA 1 cut(s) 399
MspA1I CMGCKG 3 cut(s) 209, 1377, 1705
MspI CCGG 3 cut(s) 21, 1488, 1626
MspR9I CCNGG 6 cut(s) 9, 171, 225, 945, 1474, 1530
Mva1269I GAATGC 1 cut(s) 811
MvaI CCWGG 6 cut(s) 9, 171, 225, 945, 1474, 1530
MwoI GCNNNNNNNGC 4 cut(s) 1044, 1350, 1628, 1637
NdeII GATC 4 cut(s) 102, 1096, 1289, 1659
NlaIV GGNNCC 7 cut(s) 6, 165, 344, 420, 630, 1116, 1630
NmuCI GTSAC 2 cut(s) 471, 785
NsiI ATGCAT 2 cut(s) 294, 1619
NspI RCATGY 4 cut(s) 292, 680, 1447, 1574
PaeR7I CTCGAG 1 cut(s) 1217
PagI TCATGA 3 cut(s) 456, 534, 949
PceI AGGCCT 2 cut(s) 49, 115
PciI ACATGT 3 cut(s) 676, 1443, 1570
PctI GAATGC 1 cut(s) 811
PfeI GAWTC 5 cut(s) 337, 644, 704, 914, 977
PflMI CCANNNNNTGG 2 cut(s) 731, 1458
PfoI TCCNGGA 1 cut(s) 169
PinAI ACCGGT 1 cut(s) 20
PkrI GCNGC 6 cut(s) 205, 208, 301, 1376, 1379, 1624
PleI GAGTC 4 cut(s) 188, 192, 478, 1269
PpsI GAGTC 4 cut(s) 188, 192, 478, 1269
PscI ACATGT 3 cut(s) 676, 1443, 1570
PshBI ATTAAT 1 cut(s) 1598
Psp124BI GAGCTC 1 cut(s) 36
Psp6I CCWGG 6 cut(s) 7, 169, 223, 943, 1472, 1528
PspFI CCCAGC 1 cut(s) 699
PspGI CCWGG 6 cut(s) 7, 169, 223, 943, 1472, 1528
PspN4I GGNNCC 7 cut(s) 6, 165, 344, 420, 630, 1116, 1630
PspPI GGNCC 6 cut(s) 4, 163, 343, 500, 545, 1206
PstI CTGCAG 1 cut(s) 208
PsuI RGATCY 2 cut(s) 102, 1659
PvuII CAGCTG 3 cut(s) 209, 1377, 1705
RsaI GTAC 3 cut(s) 1202, 1514, 1555
RsaNI GTAC 3 cut(s) 1201, 1513, 1554
RseI CAYNNNNRTG 1 cut(s) 783
SacI GAGCTC 1 cut(s) 36
SaqAI TTAA 3 cut(s) 252, 1383, 1598
SatI GCNGC 6 cut(s) 204, 207, 300, 1375, 1378, 1623
Sau3AI GATC 4 cut(s) 102, 1096, 1289, 1659
Sau96I GGNCC 6 cut(s) 4, 163, 343, 500, 545, 1206
ScaI AGTACT 1 cut(s) 1202
SchI GAGTC 4 cut(s) 189, 192, 479, 1269
ScrFI CCNGG 6 cut(s) 9, 171, 225, 945, 1474, 1530
SduI GDGCHC 5 cut(s) 36, 135, 1396, 1451, 1642
SexAI ACCWGGT 2 cut(s) 1472, 1528
SfaNI GCATC 1 cut(s) 1116
SfcI CTRYAG 1 cut(s) 204
Sfr274I CTCGAG 1 cut(s) 1217
SinI GGWCC 6 cut(s) 4, 163, 343, 500, 545, 1206
SlaI CTCGAG 1 cut(s) 1217
SmiMI CAYNNNNRTG 1 cut(s) 783
SmlI CTYRAG 1 cut(s) 1217
SmoI CTYRAG 1 cut(s) 1217
SseBI AGGCCT 2 cut(s) 49, 115
SsiI CCGC 2 cut(s) 685, 1342
SspMI CTAG 1 cut(s) 29
SstI GAGCTC 1 cut(s) 36
StuI AGGCCT 2 cut(s) 49, 115
StyD4I CCNGG 6 cut(s) 7, 169, 223, 943, 1472, 1528
TaaI ACNGT 3 cut(s) 1081, 1259, 1420
TaqI TCGA 4 cut(s) 178, 468, 1218, 1602
TaqII GACCGA 1 cut(s) 533
TatI WGTACW 1 cut(s) 1200
TfiI GAWTC 5 cut(s) 337, 644, 704, 914, 977
Tru1I TTAA 3 cut(s) 252, 1383, 1598
Tru9I TTAA 3 cut(s) 252, 1383, 1598
TscAI CASTG 4 cut(s) 147, 337, 823, 1639
TseFI GTSAC 2 cut(s) 471, 785
TseI GCWGC 6 cut(s) 203, 206, 299, 1374, 1377, 1622
Tsp45I GTSAC 2 cut(s) 471, 785
TspDTI ATGAA 6 cut(s) 165, 291, 445, 473, 551, 896
TspGWI ACGGA 1 cut(s) 1571
TspRI CASTG 4 cut(s) 147, 337, 823, 1639
Van91I CCANNNNNTGG 2 cut(s) 731, 1458
VneI GTGCAC 2 cut(s) 1447, 1638
VpaK11BI GGWCC 6 cut(s) 4, 163, 343, 500, 545, 1206
VspI ATTAAT 1 cut(s) 1598
XagI CCTNNNNNAGG 2 cut(s) 486, 1504
XapI RAATTY 3 cut(s) 174, 256, 651
XceI RCATGY 4 cut(s) 292, 680, 1447, 1574
XhoI CTCGAG 1 cut(s) 1217
XspI CTAG 1 cut(s) 29
ZrmI AGTACT 1 cut(s) 1202
Zsp2I ATGCAT 2 cut(s) 294, 1619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.