Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0001422

Physical Location & Seq

Genomic Coordinates Forward (+)

3334968 .. 3337819

Gene Structure 2852 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g03730.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 990 bp

ATGGCTAACAATGGCGTACGGCAGACCGGAAAGGGTATGATCGTGAGTTTCGGCGAGATGCTGATCGACTTCGTCCCCACCGAATCGGGCGTTTCTCTTGCCGAAGCTCCTGGCTTCCTCAAAGCCCCCGGCGGGGCCCCCGCCAACGTGGCGATCGCCGTGGCTCGCCTCGGCGGAAAGGCCGCGTTCGTCGGCAAACTCGGCGACGACGAGTTCGGCCACATGCTCGCCGGCATCCTGAAGCAATACGGCGTCACTGGAGATGGCATCTCGTTCGATCAGGGCGCCCGCACCGCCTTGGCCTTCGTGACCCTACGCGCCGACGGTGAACGTGAGTTCATGTTCTACCGGAACCCTAGCGCCGATATGCTGCTGAAGCCCGACGAGCTCAACATCGAGCTCATCAAATCCGCCAAAGTGTTCCACTATGGATCCATTAGCTTGATCGTGGAGCCGTGCAGATCGGCACATCTGAAGGCAATGGAGGTGGCTAAAGAAGCAGGTGCATTGCTCTCCTACGATCCGAACCTCCGGGAGCTGCTGTGGCCGTCGCGGGAAGAGGCAAAGAAGCAGATAATGAGCATATGGGATAAGGCCGATTTGATCAAGGTGAGTGATGTGGAGCTGGAGTTCCTTACAGGGAACCCCAACATTGATGATGCCAATGCCATGACTCTGTGGAGAGATAGCTTGAAGCTCCTCCTTGTCACTCTTGGTGAGAAGGGTTGCAGATACTACACCAAGAATTTCCGTGGAGTGGTGGAATCCTTCGAGGTCAAAGCCGTAGACACCACCGGTGCCGGTGATTCGTTTGTCGGTGCTCTCCTTGCGAAGATTGTCGATGATCACTCCATTCTTGAAGACGAGAAGAGGTTGAGGGAGGTACTTAAATTTGCAAATGCATGCGGAGCGATTACAACAACCAAGAAGGGAGCTATCCCCGCACTTCCCACCGAGTCTGAAGCCCTCACCCTCTTCAAAGAAAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004396 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0005975 GO:0005996 GO:0006000 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008865 GO:0009506 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019200 GO:0019318 GO:0030054 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046835 GO:0055044 GO:0071704 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00051 ko00500 ko00520 ko01100 map00051 map00500 map00520 map01100

Pfam Domains

Protein Families

PfkB

Protein Analysis

330

Amino Acids

35.62

Weight (kDa)

5.46

Isoelectric Point (pI)

24.7

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001422)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59480
fragaria_vesca	FvH4_1g23010 FvH4_1g23020 FvH4_4g10860
malus_domestica	MD04G1042400.v1.1
prunus_persica	Prupe.1G196700_v2.0.a1
pyrus_communis	pycom04g03730
rosa_chinensis	RchiOBHm_Chr2g0157021 RchiOBHm_Chr4g0406591 RchiOBHm_Chr5g0068991 RchiOBHm_Chr7g0194531 RchiOBHm_Chr7g0194641 RchiOBHm_Chr7g0194651
rosa_laevigata	RLG00000004186 RLG00000004193 RLG00000008765 RLG00000018591 RLG00000018724 RLG00000036028
rosa_multiflora	Rmu_sc0000853.1_g000003 Rmu_sc0002774.1_g000008 Rmu_sc0010033.1_g000004 Rmu_sc0025697.1_g000002 Rmu_ssc0000261.1_g000007 Rmu_ssc0000261.1_g000008 Rmu_ssc0000261.1_g000019
rosa_roxburghii	Rroxscaffold_1G00011730 Rroxscaffold_3G00261230 Rroxscaffold_3G00261270 Rroxscaffold_5G00351440
rosa_rugosa	Rorug02G0231600 Rorug04G0072300 Rorug05G0395600 Rorug07G0016200 Rorug07G0016500 Rorug07G0214800
rosa_samantha	Rh2AG288300 Rh2DG313400 Rh4BG136500 Rh4CG146600 Rh4DG133800 Rh5BG471200 Rh5CG493700 Rh5DG483900 Rh7AG143700 Rh7BG144300 Rh7BG348300 Rh7DG145600
rosa_wichuraiana	Rw0G006130 Rw2G023990 Rw5G042240 Rw7G012310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	491
Acc36I	ACCTGC	1 cut(s)	491
AccB1I	GGYRCC	2 cut(s)	284, 797
AccI	GTMKAC	1 cut(s)	786
AccII	CGCG	3 cut(s)	185, 318, 553
AclWI	GGATC	3 cut(s)	426, 439, 515
AcoI	YGGCCR	2 cut(s)	217, 545
AcsI	RAATTY	2 cut(s)	745, 890
AcuI	CTGAAG	4 cut(s)	260, 395, 494, 981
AcyI	GRCGYC	2 cut(s)	252, 285
AfaI	GTAC	2 cut(s)	18, 885
AfiI	CCNNNNNNNGG	3 cut(s)	132, 133, 757
AgeI	ACCGGT	1 cut(s)	794
AgsI	TTSAA	3 cut(s)	694, 860, 979
AjnI	CCWGG	1 cut(s)	109
AloI	GAACNNNNNNTCC	2 cut(s)	614, 646
AluBI	AGCT	9 cut(s)	107, 388, 400, 441, 538, 625, 690, 697, 935
AluI	AGCT	9 cut(s)	107, 388, 400, 441, 538, 625, 690, 697, 935
Alw21I	GWGCWC	3 cut(s)	390, 402, 823
AlwI	GGATC	3 cut(s)	426, 439, 515
AoxI	GGCC	6 cut(s)	135, 180, 217, 300, 545, 594
ApaI	GGGCCC	1 cut(s)	139
ApeKI	GCWGC	2 cut(s)	370, 538
ApoI	RAATTY	2 cut(s)	745, 890
ArsI	GACNNNNNNTTYG	2 cut(s)	197, 229
AsiGI	ACCGGT	1 cut(s)	794
AsiSI	GCGATCGC	1 cut(s)	156
AspLEI	GCGC	3 cut(s)	287, 320, 362
AspS9I	GGNCC	2 cut(s)	135, 136
AsuC2I	CCSGG	2 cut(s)	129, 533
AsuHPI	GGTGA	5 cut(s)	338, 622, 728, 815, 961
BaeGI	GKGCMC	1 cut(s)	139
BamHI	GGATCC	1 cut(s)	431
BanI	GGYRCC	2 cut(s)	284, 797
BanII	GRGCYC	3 cut(s)	139, 390, 402
BbsI	GAAGAC	1 cut(s)	867
Bbv12I	GWGCWC	3 cut(s)	390, 402, 823
BbvI	GCAGC	2 cut(s)	357, 525
BccI	CCATC	1 cut(s)	257
BceAI	ACGGC	6 cut(s)	35, 143, 265, 439, 532, 767
BcgI	CGANNNNNNTGC	2 cut(s)	256, 290
BciT130I	CCWGG	1 cut(s)	111
BclI	TGATCA	2 cut(s)	603, 844
BcnI	CCSGG	2 cut(s)	129, 533
BfaI	CTAG	1 cut(s)	357
BfoI	RGCGCY	2 cut(s)	288, 363
BfuAI	ACCTGC	1 cut(s)	491
BglI	GCCNNNNNGGC	1 cut(s)	149
BisI	GCNGC	3 cut(s)	183, 371, 539
BlsI	GCNGC	3 cut(s)	184, 372, 540
Bme1390I	CCNGG	3 cut(s)	111, 129, 533
BmgT120I	GGNCC	2 cut(s)	135, 136
BmiI	GGNNCC	9 cut(s)	136, 137, 138, 286, 353, 433, 453, 644, 799
BmrFI	CCNGG	3 cut(s)	111, 129, 533
BmsI	GCATC	4 cut(s)	48, 243, 276, 649
BpiI	GAAGAC	1 cut(s)	867
BpmI	CTGGAG	2 cut(s)	279, 647
BpuMI	CCSGG	2 cut(s)	129, 533
BsaBI	GATNNNNATC	1 cut(s)	62
BsaHI	GRCGYC	2 cut(s)	252, 285
BsaJI	CCNNGG	5 cut(s)	127, 159, 169, 297, 751
BsaWI	WCCGGW	3 cut(s)	26, 348, 794
BsaXI	ACNNNNNCTCC	4 cut(s)	527, 557, 614, 644
Bsc4I	CCNNNNNNNGG	3 cut(s)	132, 133, 757
Bse118I	RCCGGY	3 cut(s)	230, 794, 800
Bse1I	ACTGG	1 cut(s)	262
Bse3DI	GCAATG	2 cut(s)	486, 506
Bse8I	GATNNNNATC	1 cut(s)	62
BseBI	CCWGG	1 cut(s)	111
BseDI	CCNNGG	5 cut(s)	127, 159, 169, 297, 751
BseGI	GGATG	1 cut(s)	234
BseJI	GATNNNNATC	1 cut(s)	62
BseLI	CCNNNNNNNGG	3 cut(s)	132, 133, 757
BseMI	GCAATG	2 cut(s)	486, 506
BseNI	ACTGG	1 cut(s)	262
BseRI	GAGGAG	1 cut(s)	689
BseSI	GKGCMC	1 cut(s)	139
BseXI	GCAGC	2 cut(s)	357, 525
BsgI	GTGCAG	1 cut(s)	478
Bsh1236I	CGCG	3 cut(s)	185, 318, 553
Bsh1285I	CGRYCG	1 cut(s)	156
BshFI	GGCC	6 cut(s)	137, 182, 219, 302, 547, 596
BshNI	GGYRCC	2 cut(s)	284, 797
BshTI	ACCGGT	1 cut(s)	794
BsiEI	CGRYCG	1 cut(s)	156
BsiHKAI	GWGCWC	3 cut(s)	390, 402, 823
BsiSI	CCGG	7 cut(s)	27, 129, 231, 349, 532, 795, 801
BsiWI	CGTACG	1 cut(s)	16
BslFI	GGGAC	1 cut(s)	59
BslI	CCNNNNNNNGG	3 cut(s)	132, 133, 757
BsmFI	GGGAC	1 cut(s)	59
BsnI	GGCC	6 cut(s)	137, 182, 219, 302, 547, 596
Bsp120I	GGGCCC	1 cut(s)	135
Bsp1286I	GDGCHC	4 cut(s)	139, 390, 402, 823
BspANI	GGCC	6 cut(s)	137, 182, 219, 302, 547, 596
BspFNI	CGCG	3 cut(s)	185, 318, 553
BspLI	GGNNCC	9 cut(s)	136, 137, 138, 286, 353, 433, 453, 644, 799
BspMI	ACCTGC	1 cut(s)	491
BspPI	GGATC	3 cut(s)	426, 439, 515
BspT107I	GGYRCC	2 cut(s)	284, 797
BsrDI	GCAATG	2 cut(s)	486, 506
BsrFI	RCCGGY	3 cut(s)	230, 794, 800
BsrI	ACTGG	1 cut(s)	262
BssAI	RCCGGY	3 cut(s)	230, 794, 800
BssECI	CCNNGG	5 cut(s)	127, 159, 169, 297, 751
BssNI	GRCGYC	2 cut(s)	252, 285
BssT1I	CCWWGG	1 cut(s)	297
Bst2UI	CCWGG	1 cut(s)	111
Bst4CI	ACNGT	1 cut(s)	326
Bst6I	CTCTTC	3 cut(s)	552, 863, 980
BstACI	GRCGYC	2 cut(s)	252, 285
BstC8I	GCNNGC	5 cut(s)	166, 228, 232, 289, 904
BstDSI	CCRYGG	2 cut(s)	159, 751
BstF5I	GGATG	1 cut(s)	234
BstFNI	CGCG	3 cut(s)	185, 318, 553
BstH2I	RGCGCY	2 cut(s)	288, 363
BstHHI	GCGC	3 cut(s)	287, 320, 362
BstMCI	CGRYCG	1 cut(s)	156
BstNI	CCWGG	1 cut(s)	111
BstNSI	RCATGY	2 cut(s)	226, 906
BstSCI	CCNGG	3 cut(s)	109, 127, 531
BstSLI	GKGCMC	1 cut(s)	139
BstUI	CGCG	3 cut(s)	185, 318, 553
BstV1I	GCAGC	2 cut(s)	357, 525
BstV2I	GAAGAC	1 cut(s)	867
BstX2I	RGATCY	1 cut(s)	431
BstYI	RGATCY	1 cut(s)	431
BsuRI	GGCC	6 cut(s)	137, 182, 219, 302, 547, 596
BtgI	CCRYGG	2 cut(s)	159, 751
BtsCI	GGATG	1 cut(s)	234
BtsIMutI	CAGTG	1 cut(s)	255
BveI	ACCTGC	1 cut(s)	491
Cac8I	GCNNGC	5 cut(s)	166, 228, 232, 289, 904
CfoI	GCGC	3 cut(s)	287, 320, 362
Cfr10I	RCCGGY	3 cut(s)	230, 794, 800
Cfr13I	GGNCC	2 cut(s)	135, 136
CseI	GACGC	1 cut(s)	241
Csp6I	GTAC	2 cut(s)	17, 884
CspAI	ACCGGT	1 cut(s)	794
CspCI	CAANNNNNGTGG	2 cut(s)	468, 503
CviAII	CATG	4 cut(s)	223, 340, 670, 903
CviQI	GTAC	2 cut(s)	17, 884
DinI	GGCGCC	1 cut(s)	286
EaeI	YGGCCR	2 cut(s)	217, 545
Eam1104I	CTCTTC	3 cut(s)	552, 863, 980
EarI	CTCTTC	3 cut(s)	552, 863, 980
EciI	GGCGGA	2 cut(s)	189, 400
Ecl136II	GAGCTC	2 cut(s)	388, 400
Eco130I	CCWWGG	1 cut(s)	297
Eco24I	GRGCYC	3 cut(s)	139, 390, 402
Eco53kI	GAGCTC	2 cut(s)	388, 400
Eco57I	CTGAAG	4 cut(s)	260, 395, 494, 981
EcoICRI	GAGCTC	2 cut(s)	388, 400
EcoO109I	RGGNCCY	2 cut(s)	135, 136
EcoRII	CCWGG	1 cut(s)	109
EcoT14I	CCWWGG	1 cut(s)	297
EcoT22I	ATGCAT	1 cut(s)	904
EcoT38I	GRGCYC	3 cut(s)	139, 390, 402
EgeI	GGCGCC	1 cut(s)	286
EheI	GGCGCC	1 cut(s)	286
ErhI	CCWWGG	1 cut(s)	297
FaeI	CATG	4 cut(s)	226, 343, 673, 906
FaiI	YATR	9 cut(s)	38, 224, 341, 368, 429, 584, 586, 671, 904
FaqI	GGGAC	1 cut(s)	59
FatI	CATG	4 cut(s)	222, 339, 669, 902
FauI	CCCGC	5 cut(s)	125, 148, 296, 546, 949
FauNDI	CATATG	1 cut(s)	584
FbaI	TGATCA	2 cut(s)	603, 844
FblI	GTMKAC	1 cut(s)	786
Fnu4HI	GCNGC	3 cut(s)	183, 371, 539
FokI	GGATG	1 cut(s)	221
FriOI	GRGCYC	3 cut(s)	139, 390, 402
Fsp4HI	GCNGC	3 cut(s)	183, 371, 539
FspBI	CTAG	1 cut(s)	357
GlaI	GCGC	3 cut(s)	286, 319, 361
GluI	GCNGC	3 cut(s)	183, 371, 539
GsuI	CTGGAG	2 cut(s)	279, 647
HaeII	RGCGCY	2 cut(s)	288, 363
HaeIII	GGCC	6 cut(s)	137, 182, 219, 302, 547, 596
HapII	CCGG	7 cut(s)	27, 129, 231, 349, 532, 795, 801
HgaI	GACGC	1 cut(s)	241
HhaI	GCGC	3 cut(s)	287, 320, 362
Hin1I	GRCGYC	2 cut(s)	252, 285
Hin1II	CATG	4 cut(s)	226, 343, 673, 906
Hin6I	GCGC	3 cut(s)	285, 318, 360
HinP1I	GCGC	3 cut(s)	285, 318, 360
HinfI	GANTC	5 cut(s)	83, 673, 764, 806, 956
HpaII	CCGG	7 cut(s)	27, 129, 231, 349, 532, 795, 801
HphI	GGTGA	5 cut(s)	338, 622, 728, 815, 961
Hpy166II	GTNNAC	2 cut(s)	329, 787
Hpy188I	TCNGA	3 cut(s)	474, 525, 961
Hpy188III	TCNNGA	4 cut(s)	43, 238, 307, 857
Hpy8I	GTNNAC	2 cut(s)	329, 787
Hpy99I	CGWCG	6 cut(s)	194, 209, 212, 326, 386, 553
HpyAV	CCTTC	5 cut(s)	313, 469, 715, 778, 922
HpyCH4III	ACNGT	1 cut(s)	326
HpyCH4IV	ACGT	2 cut(s)	147, 331
HpyCH4V	TGCA	5 cut(s)	459, 506, 729, 896, 902
HpySE526I	ACGT	2 cut(s)	147, 331
Hsp92I	GRCGYC	2 cut(s)	252, 285
Hsp92II	CATG	4 cut(s)	226, 343, 673, 906
HspAI	GCGC	3 cut(s)	285, 318, 360
KasI	GGCGCC	1 cut(s)	284
KroI	GCCGGC	1 cut(s)	230
KroNI	GCCGGC	1 cut(s)	232
Ksp22I	TGATCA	2 cut(s)	603, 844
LmnI	GCTCC	7 cut(s)	112, 451, 535, 622, 702, 908, 932
Lsp1109I	GCAGC	2 cut(s)	357, 525
LweI	GCATC	4 cut(s)	48, 243, 276, 649
MaeI	CTAG	1 cut(s)	357
MaeII	ACGT	2 cut(s)	147, 331
MaeIII	GTNAC	3 cut(s)	253, 307, 706
MboII	GAAGA	5 cut(s)	569, 844, 872, 880, 967
MflI	RGATCY	1 cut(s)	431
MhlI	GDGCHC	4 cut(s)	139, 390, 402, 823
MluCI	AATT	2 cut(s)	745, 890
Mly113I	GGCGCC	1 cut(s)	285
MlyI	GAGTC	2 cut(s)	667, 965
Mph1103I	ATGCAT	1 cut(s)	904
MroNI	GCCGGC	1 cut(s)	230
MseI	TTAA	1 cut(s)	888
MspI	CCGG	7 cut(s)	27, 129, 231, 349, 532, 795, 801
MspR9I	CCNGG	3 cut(s)	111, 129, 533
MvaI	CCWGG	1 cut(s)	111
MvnI	CGCG	3 cut(s)	185, 318, 553
NaeI	GCCGGC	1 cut(s)	232
NarI	GGCGCC	1 cut(s)	285
NciI	CCSGG	2 cut(s)	129, 533
NdeI	CATATG	1 cut(s)	584
NgoMIV	GCCGGC	1 cut(s)	230
NlaIII	CATG	4 cut(s)	226, 343, 673, 906
NlaIV	GGNNCC	9 cut(s)	136, 137, 138, 286, 353, 433, 453, 644, 799
NmeAIII	GCCGAG	2 cut(s)	150, 180
NmuCI	GTSAC	3 cut(s)	253, 307, 706
NsiI	ATGCAT	1 cut(s)	904
NspI	RCATGY	2 cut(s)	226, 906
PaeI	GCATGC	1 cut(s)	906
PaqCI	CACCTGC	1 cut(s)	491
PcsI	WCGNNNNNNNCGW	2 cut(s)	78, 207
PdiI	GCCGGC	1 cut(s)	232
PfeI	GAWTC	3 cut(s)	83, 764, 806
Pfl23II	CGTACG	1 cut(s)	16
PflFI	GACNNNGTC	1 cut(s)	71
PfoI	TCCNGGA	1 cut(s)	531
PinAI	ACCGGT	1 cut(s)	794
PkrI	GCNGC	3 cut(s)	184, 372, 540
Ple19I	CGATCG	1 cut(s)	156
PleI	GAGTC	2 cut(s)	667, 964
PluTI	GGCGCC	1 cut(s)	288
PpsI	GAGTC	2 cut(s)	667, 964
Psp124BI	GAGCTC	2 cut(s)	390, 402
Psp6I	CCWGG	1 cut(s)	109
PspGI	CCWGG	1 cut(s)	109
PspLI	CGTACG	1 cut(s)	16
PspN4I	GGNNCC	9 cut(s)	136, 137, 138, 286, 353, 433, 453, 644, 799
PspOMI	GGGCCC	1 cut(s)	135
PspPI	GGNCC	2 cut(s)	135, 136
PsuI	RGATCY	1 cut(s)	431
PsyI	GACNNNGTC	1 cut(s)	71
PvuI	CGATCG	1 cut(s)	156
RgaI	GCGATCGC	1 cut(s)	156
RsaI	GTAC	2 cut(s)	18, 885
RsaNI	GTAC	2 cut(s)	17, 884
SacI	GAGCTC	2 cut(s)	390, 402
SaqAI	TTAA	1 cut(s)	888
SatI	GCNGC	3 cut(s)	183, 371, 539
Sau96I	GGNCC	2 cut(s)	135, 136
SchI	GAGTC	2 cut(s)	667, 965
ScrFI	CCNGG	3 cut(s)	111, 129, 533
SduI	GDGCHC	4 cut(s)	139, 390, 402, 823
SfaAI	GCGATCGC	1 cut(s)	156
SfaNI	GCATC	4 cut(s)	48, 243, 276, 649
SfoI	GGCGCC	1 cut(s)	286
SgfI	GCGATCGC	1 cut(s)	156
SgrAI	CRCCGGYG	1 cut(s)	794
SphI	GCATGC	1 cut(s)	906
Sse9I	AATT	2 cut(s)	745, 890
SspDI	GGCGCC	1 cut(s)	284
SspMI	CTAG	1 cut(s)	357
SstI	GAGCTC	2 cut(s)	390, 402
StyD4I	CCNGG	3 cut(s)	109, 127, 531
StyI	CCWWGG	1 cut(s)	297
TaaI	ACNGT	1 cut(s)	326
TaiI	ACGT	2 cut(s)	150, 334
TaqI	TCGA	5 cut(s)	66, 276, 396, 771, 840
TasI	AATT	2 cut(s)	745, 890
TauI	GCSGC	1 cut(s)	185
TfiI	GAWTC	3 cut(s)	83, 764, 806
Tru1I	TTAA	1 cut(s)	888
Tru9I	TTAA	1 cut(s)	888
TscAI	CASTG	1 cut(s)	262
TseFI	GTSAC	3 cut(s)	253, 307, 706
TseI	GCWGC	2 cut(s)	370, 538
Tsp45I	GTSAC	3 cut(s)	253, 307, 706
TspDTI	ATGAA	1 cut(s)	328
TspGWI	ACGGA	1 cut(s)	740
TspRI	CASTG	1 cut(s)	262
Tth111I	GACNNNGTC	1 cut(s)	71
XapI	RAATTY	2 cut(s)	745, 890
XceI	RCATGY	2 cut(s)	226, 906
XmiI	GTMKAC	1 cut(s)	786
XspI	CTAG	1 cut(s)	357
Zsp2I	ATGCAT	1 cut(s)	904

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.