Prupe.6G059100_v2.0.a1

Pleckstrin homology domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0009844
Physical Location & Seq
Reverse (-)
4107297 .. 4107788
492 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G059100.1

Sequence Viewer

Length: 438 bp
ATGGAGGGGCTTTGGCGGGCCGCGACGGGCCAAGACCCGAGCCCAGAAGACTACACAGGGATAGAGTTCTGGTCCAACCCGGAACGCGCCACGTGGCTGACCAAGCAAGGCGAGTACATCAAGACCTGGCGGCGCCGCTGGTTCGTCTTGAAACAAGGGAAGCTGTTCTGGTTCAAGGATGGTCACGTTACCCGCACGTCGACACCGCGCGGCGTGATCCCGGTGGGCACGTGCCTGACGGTGAAGGGCGCCGAGGACGTGGTCCACAAGCCTTGCGCCTTCGAGCTCTCCACCAACACCCACGACACCATGTACTTCATCGCCGACTCGGAGAAGGAGAAGGAGGAGTGGATCAACTCCATCGGACGGTCCATAGTCCAGCACTCGAGATCGCTCGCGGATTCCGAGGTTGTCGACTACGATAGCCACCGAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PH

Protein Analysis

146

Amino Acids

16.72

Weight (kDa)

6.2

Isoelectric Point (pI)

44.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 132, 248
AccI GTMKAC 2 cut(s) 200, 414
AccII CGCG 5 cut(s) 23, 87, 208, 210, 398
AciI CCGC 8 cut(s) 16, 21, 130, 136, 193, 206, 210, 398
AclWI GGATC 2 cut(s) 211, 359
AcvI CACGTG 2 cut(s) 93, 231
AcyI GRCGYC 2 cut(s) 133, 249
AfaI GTAC 2 cut(s) 116, 314
AfiI CCNNNNNNNGG 1 cut(s) 366
AgsI TTSAA 2 cut(s) 151, 175
AjiI CACGTC 2 cut(s) 198, 259
AjnI CCWGG 1 cut(s) 125
AluBI AGCT 2 cut(s) 163, 286
AluI AGCT 2 cut(s) 163, 286
Alw21I GWGCWC 1 cut(s) 288
AlwI GGATC 2 cut(s) 211, 359
Ama87I CYCGRG 2 cut(s) 37, 385
AoxI GGCC 2 cut(s) 18, 28
Asp700I GAANNNNTTC 1 cut(s) 164
AspLEI GCGC 5 cut(s) 89, 135, 210, 251, 278
AspS9I GGNCC 5 cut(s) 18, 28, 72, 262, 369
AsuC2I CCSGG 2 cut(s) 80, 221
AsuHPI GGTGA 1 cut(s) 253
AvaI CYCGRG 2 cut(s) 37, 385
AvaII GGWCC 3 cut(s) 72, 262, 369
BaeGI GKGCMC 1 cut(s) 230
BanI GGYRCC 2 cut(s) 132, 248
BanII GRGCYC 2 cut(s) 44, 288
BbrPI CACGTG 2 cut(s) 93, 231
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 288
BccI CCATC 2 cut(s) 173, 368
BciT130I CCWGG 1 cut(s) 127
BcnI CCSGG 2 cut(s) 80, 221
BfoI RGCGCY 2 cut(s) 136, 252
BisI GCNGC 4 cut(s) 21, 131, 136, 211
BlsI GCNGC 4 cut(s) 22, 132, 137, 212
Bme1390I CCNGG 3 cut(s) 80, 127, 221
Bme18I GGWCC 3 cut(s) 72, 262, 369
BmeT110I CYCGRG 2 cut(s) 37, 385
BmgBI CACGTC 2 cut(s) 198, 259
BmgT120I GGNCC 5 cut(s) 18, 28, 72, 262, 369
BmiI GGNNCC 2 cut(s) 134, 250
BmrFI CCNGG 3 cut(s) 80, 127, 221
BpiI GAAGAC 1 cut(s) 54
BpuMI CCSGG 2 cut(s) 80, 221
BsaAI YACGTR 2 cut(s) 93, 231
BsaHI GRCGYC 2 cut(s) 133, 249
BsaJI CCNNGG 3 cut(s) 252, 405, 430
Bsc4I CCNNNNNNNGG 1 cut(s) 366
BseBI CCWGG 1 cut(s) 127
BseDI CCNNGG 3 cut(s) 252, 405, 430
BseGI GGATG 1 cut(s) 184
BseLI CCNNNNNNNGG 1 cut(s) 366
BseRI GAGGAG 1 cut(s) 359
BseSI GKGCMC 1 cut(s) 230
Bsh1236I CGCG 5 cut(s) 23, 87, 208, 210, 398
BshFI GGCC 2 cut(s) 20, 30
BshNI GGYRCC 2 cut(s) 132, 248
BsiHKAI GWGCWC 1 cut(s) 288
BsiHKCI CYCGRG 2 cut(s) 37, 385
BsiSI CCGG 2 cut(s) 80, 221
BslI CCNNNNNNNGG 1 cut(s) 366
BsnI GGCC 2 cut(s) 20, 30
BsoBI CYCGRG 2 cut(s) 37, 385
Bsp1286I GDGCHC 3 cut(s) 44, 230, 288
Bsp143I GATC 3 cut(s) 216, 351, 389
BspACI CCGC 8 cut(s) 16, 21, 130, 136, 193, 206, 210, 398
BspANI GGCC 2 cut(s) 20, 30
BspFNI CGCG 5 cut(s) 23, 87, 208, 210, 398
BspLI GGNNCC 2 cut(s) 134, 250
BspPI GGATC 2 cut(s) 211, 359
BspT107I GGYRCC 2 cut(s) 132, 248
BssECI CCNNGG 3 cut(s) 252, 405, 430
BssMI GATC 3 cut(s) 216, 351, 389
BssNI GRCGYC 2 cut(s) 133, 249
Bst2UI CCWGG 1 cut(s) 127
Bst4CI ACNGT 2 cut(s) 241, 369
BstACI GRCGYC 2 cut(s) 133, 249
BstBAI YACGTR 2 cut(s) 93, 231
BstC8I GCNNGC 2 cut(s) 18, 396
BstF5I GGATG 1 cut(s) 184
BstFNI CGCG 5 cut(s) 23, 87, 208, 210, 398
BstH2I RGCGCY 2 cut(s) 136, 252
BstHHI GCGC 5 cut(s) 89, 135, 210, 251, 278
BstKTI GATC 3 cut(s) 219, 354, 392
BstMBI GATC 3 cut(s) 216, 351, 389
BstMWI GCNNNNNNNGC 1 cut(s) 103
BstNI CCWGG 1 cut(s) 127
BstSCI CCNGG 3 cut(s) 78, 125, 219
BstSLI GKGCMC 1 cut(s) 230
BstUI CGCG 5 cut(s) 23, 87, 208, 210, 398
BstV2I GAAGAC 1 cut(s) 54
BsuRI GGCC 2 cut(s) 20, 30
BtgZI GCGATG 1 cut(s) 304
BtrI CACGTC 2 cut(s) 198, 259
BtsCI GGATG 1 cut(s) 184
Cac8I GCNNGC 2 cut(s) 18, 396
CfoI GCGC 5 cut(s) 89, 135, 210, 251, 278
Cfr13I GGNCC 5 cut(s) 18, 28, 72, 262, 369
Csp6I GTAC 2 cut(s) 115, 313
CspCI CAANNNNNGTGG 2 cut(s) 254, 289
CviAII CATG 1 cut(s) 310
CviJI RGCY 9 cut(s) 10, 20, 30, 42, 97, 163, 271, 286, 426
CviKI_1 RGCY 9 cut(s) 10, 20, 30, 42, 97, 163, 271, 286, 426
CviQI GTAC 2 cut(s) 115, 313
DinI GGCGCC 2 cut(s) 134, 250
DpnI GATC 3 cut(s) 218, 353, 391
DpnII GATC 3 cut(s) 216, 351, 389
Ecl136II GAGCTC 1 cut(s) 286
Eco24I GRGCYC 2 cut(s) 44, 288
Eco47I GGWCC 3 cut(s) 72, 262, 369
Eco53kI GAGCTC 1 cut(s) 286
Eco72I CACGTG 2 cut(s) 93, 231
Eco88I CYCGRG 2 cut(s) 37, 385
EcoICRI GAGCTC 1 cut(s) 286
EcoRII CCWGG 1 cut(s) 125
EcoT38I GRGCYC 2 cut(s) 44, 288
EgeI GGCGCC 2 cut(s) 134, 250
EheI GGCGCC 2 cut(s) 134, 250
FaeI CATG 1 cut(s) 313
FaiI YATR 2 cut(s) 311, 374
FatI CATG 1 cut(s) 309
FauI CCCGC 2 cut(s) 9, 200
FblI GTMKAC 2 cut(s) 200, 414
Fnu4HI GCNGC 4 cut(s) 21, 131, 136, 211
FokI GGATG 1 cut(s) 191
FriOI GRGCYC 2 cut(s) 44, 288
Fsp4HI GCNGC 4 cut(s) 21, 131, 136, 211
GlaI GCGC 5 cut(s) 88, 134, 209, 250, 277
GluI GCNGC 4 cut(s) 21, 131, 136, 211
HaeII RGCGCY 2 cut(s) 136, 252
HaeIII GGCC 2 cut(s) 20, 30
HapII CCGG 2 cut(s) 80, 221
HhaI GCGC 5 cut(s) 89, 135, 210, 251, 278
Hin1I GRCGYC 2 cut(s) 133, 249
Hin1II CATG 1 cut(s) 313
Hin6I GCGC 5 cut(s) 87, 133, 208, 249, 276
HinP1I GCGC 5 cut(s) 87, 133, 208, 249, 276
HincII GTYRAC 2 cut(s) 201, 415
HindII GTYRAC 2 cut(s) 201, 415
HinfI GANTC 2 cut(s) 326, 401
HpaII CCGG 2 cut(s) 80, 221
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 3 cut(s) 201, 265, 415
Hpy188I TCNGA 3 cut(s) 331, 365, 406
Hpy188III TCNNGA 3 cut(s) 121, 148, 387
Hpy8I GTNNAC 3 cut(s) 201, 265, 415
Hpy99I CGWCG 2 cut(s) 28, 202
HpyAV CCTTC 4 cut(s) 238, 289, 328, 334
HpyCH4III ACNGT 2 cut(s) 241, 369
HpyCH4IV ACGT 5 cut(s) 92, 186, 197, 230, 258
HpyF10VI GCNNNNNNNGC 1 cut(s) 103
HpySE526I ACGT 5 cut(s) 92, 186, 197, 230, 258
Hsp92I GRCGYC 2 cut(s) 133, 249
Hsp92II CATG 1 cut(s) 313
HspAI GCGC 5 cut(s) 87, 133, 208, 249, 276
KasI GGCGCC 2 cut(s) 132, 248
Kzo9I GATC 3 cut(s) 216, 351, 389
MaeII ACGT 5 cut(s) 92, 186, 197, 230, 258
MaeIII GTNAC 2 cut(s) 182, 187
MalI GATC 3 cut(s) 218, 353, 391
MboI GATC 3 cut(s) 216, 351, 389
MboII GAAGA 1 cut(s) 59
MhlI GDGCHC 3 cut(s) 44, 230, 288
Mly113I GGCGCC 2 cut(s) 133, 249
MlyI GAGTC 1 cut(s) 320
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 4 cut(s) 247, 337, 400, 425
MroXI GAANNNNTTC 1 cut(s) 164
MspA1I CMGCKG 1 cut(s) 138
MspI CCGG 2 cut(s) 80, 221
MspR9I CCNGG 3 cut(s) 80, 127, 221
MvaI CCWGG 1 cut(s) 127
MvnI CGCG 5 cut(s) 23, 87, 208, 210, 398
MwoI GCNNNNNNNGC 1 cut(s) 103
NarI GGCGCC 2 cut(s) 133, 249
NciI CCSGG 2 cut(s) 80, 221
NdeII GATC 3 cut(s) 216, 351, 389
NlaIII CATG 1 cut(s) 313
NlaIV GGNNCC 2 cut(s) 134, 250
NmeAIII GCCGAG 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 182
PaeR7I CTCGAG 1 cut(s) 385
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PdmI GAANNNNTTC 1 cut(s) 164
PfeI GAWTC 1 cut(s) 401
PflFI GACNNNGTC 1 cut(s) 260
PkrI GCNGC 4 cut(s) 22, 132, 137, 212
PleI GAGTC 1 cut(s) 320
PluTI GGCGCC 2 cut(s) 136, 252
PmaCI CACGTG 2 cut(s) 93, 231
PmlI CACGTG 2 cut(s) 93, 231
PpsI GAGTC 1 cut(s) 320
Ppu21I YACGTR 2 cut(s) 93, 231
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 125
PspCI CACGTG 2 cut(s) 93, 231
PspGI CCWGG 1 cut(s) 125
PspN4I GGNNCC 2 cut(s) 134, 250
PspPI GGNCC 5 cut(s) 18, 28, 72, 262, 369
PsyI GACNNNGTC 1 cut(s) 260
RsaI GTAC 2 cut(s) 116, 314
RsaNI GTAC 2 cut(s) 115, 313
SacI GAGCTC 1 cut(s) 288
SalI GTCGAC 2 cut(s) 199, 413
SatI GCNGC 4 cut(s) 21, 131, 136, 211
Sau3AI GATC 3 cut(s) 216, 351, 389
Sau96I GGNCC 5 cut(s) 18, 28, 72, 262, 369
SchI GAGTC 1 cut(s) 320
ScrFI CCNGG 3 cut(s) 80, 127, 221
SduI GDGCHC 3 cut(s) 44, 230, 288
SetI ASST 9 cut(s) 95, 128, 165, 189, 200, 233, 261, 288, 411
SfoI GGCGCC 2 cut(s) 134, 250
Sfr274I CTCGAG 1 cut(s) 385
SinI GGWCC 3 cut(s) 72, 262, 369
SlaI CTCGAG 1 cut(s) 385
SmlI CTYRAG 1 cut(s) 385
SmoI CTYRAG 1 cut(s) 385
SsiI CCGC 8 cut(s) 16, 21, 130, 136, 193, 206, 210, 398
SspDI GGCGCC 2 cut(s) 132, 248
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 3 cut(s) 78, 125, 219
TaaI ACNGT 2 cut(s) 241, 369
TaiI ACGT 5 cut(s) 95, 189, 200, 233, 261
TaqI TCGA 4 cut(s) 200, 282, 386, 414
TatI WGTACW 2 cut(s) 114, 312
TauI GCSGC 4 cut(s) 23, 133, 138, 213
TfiI GAWTC 1 cut(s) 401
TseFI GTSAC 1 cut(s) 182
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 1 cut(s) 307
Tth111I GACNNNGTC 1 cut(s) 260
VpaK11BI GGWCC 3 cut(s) 72, 262, 369
XhoI CTCGAG 1 cut(s) 385
XmiI GTMKAC 2 cut(s) 200, 414
XmnI GAANNNNTTC 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.