FvH4_3g38680

Phosphoinositide phospholipase C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010550
Physical Location & Seq
Forward (+)
32981368 .. 32986677
5310 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g38680.t3

Sequence Viewer

Length: 1131 bp
ATGTCCAACAATAAGCAGTCTTTCAAGGTCTGCTTCTGTTTCAGAAGAATATTCAGGCTCAAGGTGGCAGAGCCTCCAGAAGACATCAAGAGAATGTTTAATATGTATGCCGAAAATGGCACCATCTCCCTCGATGACCTTCACAGCTTTCTGGTTGAGTTTCAAGGAGAAGGTGAGGCGACCAAAGAAGATGCACAGGCCATTTTCAACAGTCTCAAGCATCTCAACATTTTCCAAAGAAAGGGCCTCCATCTTGATGCCTTCTTTCGGTATCTTTTTGGTGATCTGAACCCTCCAATCTCTTCAAAGGTGCATCATGACATGAAAGCTCCATTGGCTCATTATTTCCTATTCACCGGCCATAACTCGTACCTGACTGGAAATCAACTCAGCAGTGACAGTAGTGTCTATCCCATTATCCAGGCACTCCAACGAGGAGTTCGAGTTATTGAATTAGATTTGTGGCCTAATTCTAAAAAGAATGGCGTCGAGGTTCGTCATGGAGGGACTTTGACTAGTCCGGTTGAACTCATCAAATGCTTGCGTGCTATTAAGGACAATGCTTTTACTGCTTCTGAATATCCAGTTGTAATAACATTTGAAGATCACTTACCTTCTGATCTTCAAGCTAAAGTGGCCAAGATGGTGACTGAAACATTTGGAGATATGCTCTACGTCCCTAATTCGGAATTTTTACCGGAATTCCCTTCACCTGAAATATTAAAAAGAAGGGTTTTGATTTCAACCAAGCCACCGGAGTATCATGAATCTCAAAGGCGCACTGATAAGGATAGTGACGAAGATGATCCAGATGAAGATGAGGAGAATTCTACTCTACAGTATAAGCAGTTAATTGCAATCCATGCTGGAAAACCAAAAGGGGGTTTTGATATGTGGCATATTGATCCCATGAAAGTCAGACGACTTAGCTTGAGCGAGCAACAACTCGAACGTGCATCGAAAAATCGTGGAACAGATATTGTTAGATTTACACAAAGAAATTTGTTAAGGATATATCCCAAGGGTACACGTTTGGACTCATCAAATTATGACCCTATGCTTGGATGGAGCCATGGAGCTCAAATGGTCGCATTCAATATGCAAGTTAGACTTCTTTCACTTGTAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004620 GO:0004629 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006970 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0008081 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009553 GO:0009555 GO:0009556 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010600 GO:0010601 GO:0010817 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0034293 GO:0042221 GO:0042578 GO:0042742 GO:0043207 GO:0043934 GO:0044464 GO:0044703 GO:0046885 GO:0046886 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071944 GO:0090354 GO:0090355 GO:0090567 GO:0097305 GO:0097306 GO:0098542 GO:0099402 GO:1901700 GO:1901701 GO:1903046
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933
Pfam Domains
Protein Families
C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

377

Amino Acids

43.14

Weight (kDa)

6.68

Isoelectric Point (pI)

51.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 108 - 250 2.8e-50 Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-Y PF00387 309 - 395 1.6e-29 Phosphatidylinositol-specific phospholipase C, Y domain
C2 PF00168 418 - 518 1.8e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 404
AccB1I GGYRCC 1 cut(s) 119
AclWI GGATC 2 cut(s) 800, 899
AcoI YGGCCR 2 cut(s) 358, 636
AcsI RAATTY 4 cut(s) 689, 701, 826, 1000
AcyI GRCGYC 1 cut(s) 486
AfaI GTAC 2 cut(s) 371, 1027
AfiI CCNNNNNNNGG 5 cut(s) 241, 267, 685, 881, 1061
AflIII ACRYGT 1 cut(s) 1028
AhlI ACTAGT 1 cut(s) 515
AjnI CCWGG 1 cut(s) 420
AjuI GAANNNNNNNTTGG 4 cut(s) 317, 349, 423, 455
AluBI AGCT 5 cut(s) 147, 329, 629, 930, 1079
AluI AGCT 5 cut(s) 147, 329, 629, 930, 1079
Alw21I GWGCWC 1 cut(s) 1081
Alw26I GTCTC 1 cut(s) 218
AlwI GGATC 2 cut(s) 800, 899
AoxI GGCC 5 cut(s) 198, 244, 358, 464, 636
ApoI RAATTY 4 cut(s) 689, 701, 826, 1000
AspLEI GCGC 1 cut(s) 780
AspS9I GGNCC 1 cut(s) 244
AsuHPI GGTGA 5 cut(s) 185, 293, 346, 658, 702
BaeI ACNNNNGTAYC 2 cut(s) 743, 776
BalI TGGCCA 1 cut(s) 638
BanI GGYRCC 1 cut(s) 119
BanII GRGCYC 1 cut(s) 1081
BarI GAAGNNNNNNTAC 2 cut(s) 594, 626
BbsI GAAGAC 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 1081
BccI CCATC 4 cut(s) 131, 258, 637, 1059
BciT130I CCWGG 1 cut(s) 422
BcoDI GTCTC 1 cut(s) 218
BcuI ACTAGT 1 cut(s) 515
BfaI CTAG 1 cut(s) 516
BfmI CTRYAG 1 cut(s) 836
Bme1390I CCNGG 1 cut(s) 422
BmgT120I GGNCC 1 cut(s) 244
BmiI GGNNCC 2 cut(s) 121, 1070
BmrFI CCNGG 1 cut(s) 422
BmsI GCATC 5 cut(s) 181, 229, 247, 322, 965
BpiI GAAGAC 1 cut(s) 87
BplI GAGNNNNNCTC 2 cut(s) 654, 686
BpmI CTGGAG 1 cut(s) 60
BpuEI CTTGAG 3 cut(s) 44, 200, 952
BsaHI GRCGYC 1 cut(s) 486
BsaJI CCNNGG 2 cut(s) 1020, 1072
BsaWI WCCGGW 3 cut(s) 520, 697, 754
BsaXI ACNNNNNCTCC 2 cut(s) 429, 459
Bsc4I CCNNNNNNNGG 5 cut(s) 241, 267, 685, 881, 1061
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 2 cut(s) 382, 584
BseBI CCWGG 1 cut(s) 422
BseDI CCNNGG 2 cut(s) 1020, 1072
BseGI GGATG 1 cut(s) 1070
BseLI CCNNNNNNNGG 5 cut(s) 241, 267, 685, 881, 1061
BseMII CTCAG 1 cut(s) 403
BseNI ACTGG 2 cut(s) 382, 584
BseRI GAGGAG 2 cut(s) 450, 836
BshFI GGCC 5 cut(s) 200, 246, 360, 466, 638
BshNI GGYRCC 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 1081
BsiSI CCGG 4 cut(s) 357, 521, 698, 755
BslFI GGGAC 2 cut(s) 520, 662
BslI CCNNNNNNNGG 5 cut(s) 241, 267, 685, 881, 1061
BsmAI GTCTC 1 cut(s) 218
BsmFI GGGAC 2 cut(s) 520, 662
BsmI GAATGC 1 cut(s) 1091
BsnI GGCC 5 cut(s) 200, 246, 360, 466, 638
Bsp1286I GDGCHC 1 cut(s) 1081
Bsp143I GATC 5 cut(s) 283, 604, 619, 805, 904
Bsp19I CCATGG 1 cut(s) 1072
BspANI GGCC 5 cut(s) 200, 246, 360, 466, 638
BspCNI CTCAG 1 cut(s) 402
BspHI TCATGA 2 cut(s) 316, 763
BspLI GGNNCC 2 cut(s) 121, 1070
BspPI GGATC 2 cut(s) 800, 899
BspT107I GGYRCC 1 cut(s) 119
BsrFI RCCGGY 1 cut(s) 356
BsrI ACTGG 2 cut(s) 382, 584
BssAI RCCGGY 1 cut(s) 356
BssECI CCNNGG 2 cut(s) 1020, 1072
BssMI GATC 5 cut(s) 283, 604, 619, 805, 904
BssNI GRCGYC 1 cut(s) 486
BssT1I CCWWGG 2 cut(s) 1020, 1072
Bst2UI CCWGG 1 cut(s) 422
Bst4CI ACNGT 3 cut(s) 212, 401, 840
Bst6I CTCTTC 1 cut(s) 307
BstACI GRCGYC 1 cut(s) 486
BstAPI GCANNNNNTGC 1 cut(s) 863
BstC8I GCNNGC 3 cut(s) 542, 546, 938
BstDEI CTNAG 2 cut(s) 389, 926
BstDSI CCRYGG 1 cut(s) 1072
BstF5I GGATG 1 cut(s) 1070
BstHHI GCGC 1 cut(s) 780
BstKTI GATC 5 cut(s) 286, 607, 622, 808, 907
BstMAI GTCTC 1 cut(s) 218
BstMBI GATC 5 cut(s) 283, 604, 619, 805, 904
BstMWI GCNNNNNNNGC 4 cut(s) 335, 569, 635, 863
BstNI CCWGG 1 cut(s) 422
BstSCI CCNGG 1 cut(s) 420
BstSFI CTRYAG 1 cut(s) 836
BstV2I GAAGAC 1 cut(s) 87
BsuRI GGCC 5 cut(s) 200, 246, 360, 466, 638
BtgI CCRYGG 1 cut(s) 1072
BtsCI GGATG 1 cut(s) 1070
BtsI GCAGTG 1 cut(s) 400
BtsIMutI CAGTG 2 cut(s) 400, 780
Cac8I GCNNGC 3 cut(s) 542, 546, 938
CciI TCATGA 2 cut(s) 316, 763
CfoI GCGC 1 cut(s) 780
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 1 cut(s) 244
CseI GACGC 1 cut(s) 475
Csp6I GTAC 2 cut(s) 370, 1026
CviAII CATG 7 cut(s) 317, 322, 500, 764, 863, 910, 1073
CviQI GTAC 2 cut(s) 370, 1026
DdeI CTNAG 2 cut(s) 389, 926
DpnI GATC 5 cut(s) 285, 606, 621, 807, 906
DpnII GATC 5 cut(s) 283, 604, 619, 805, 904
DrdI GACNNNNNNGTC 1 cut(s) 404
DseDI GACNNNNNNGTC 1 cut(s) 404
EaeI YGGCCR 2 cut(s) 358, 636
Eam1104I CTCTTC 1 cut(s) 307
EarI CTCTTC 1 cut(s) 307
Ecl136II GAGCTC 1 cut(s) 1079
Eco130I CCWWGG 2 cut(s) 1020, 1072
Eco24I GRGCYC 1 cut(s) 1081
Eco53kI GAGCTC 1 cut(s) 1079
EcoICRI GAGCTC 1 cut(s) 1079
EcoO109I RGGNCCY 1 cut(s) 244
EcoRI GAATTC 2 cut(s) 701, 826
EcoRII CCWGG 1 cut(s) 420
EcoT14I CCWWGG 2 cut(s) 1020, 1072
EcoT38I GRGCYC 1 cut(s) 1081
ErhI CCWWGG 2 cut(s) 1020, 1072
FaeI CATG 7 cut(s) 320, 325, 503, 767, 866, 913, 1076
FalI AAGNNNNNCTT 4 cut(s) 17, 49, 1095, 1127
FaqI GGGAC 2 cut(s) 520, 662
FatI CATG 7 cut(s) 316, 321, 499, 763, 862, 909, 1072
FokI GGATG 1 cut(s) 1077
FriOI GRGCYC 1 cut(s) 1081
FspBI CTAG 1 cut(s) 516
GlaI GCGC 1 cut(s) 779
GsuI CTGGAG 1 cut(s) 60
HaeIII GGCC 5 cut(s) 200, 246, 360, 466, 638
HapII CCGG 4 cut(s) 357, 521, 698, 755
HgaI GACGC 1 cut(s) 475
HhaI GCGC 1 cut(s) 780
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 7 cut(s) 320, 325, 503, 767, 866, 913, 1076
Hin6I GCGC 1 cut(s) 778
HinP1I GCGC 1 cut(s) 778
HinfI GANTC 2 cut(s) 767, 1037
HpaII CCGG 4 cut(s) 357, 521, 698, 755
HphI GGTGA 5 cut(s) 185, 293, 346, 658, 702
Hpy166II GTNNAC 1 cut(s) 1028
Hpy188I TCNGA 6 cut(s) 44, 288, 577, 619, 688, 920
Hpy188III TCNNGA 6 cut(s) 77, 88, 254, 317, 764, 809
Hpy8I GTNNAC 1 cut(s) 1028
Hpy99I CGWCG 1 cut(s) 491
HpyAV CCTTC 6 cut(s) 149, 164, 271, 624, 717, 723
HpyCH4III ACNGT 3 cut(s) 212, 401, 840
HpyCH4IV ACGT 3 cut(s) 675, 952, 1030
HpyCH4V TGCA 5 cut(s) 194, 313, 857, 956, 1102
HpyF10VI GCNNNNNNNGC 4 cut(s) 335, 569, 635, 863
HpyF3I CTNAG 2 cut(s) 389, 926
HpySE526I ACGT 3 cut(s) 675, 952, 1030
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 7 cut(s) 320, 325, 503, 767, 866, 913, 1076
HspAI GCGC 1 cut(s) 778
Kzo9I GATC 5 cut(s) 283, 604, 619, 805, 904
LmnI GCTCC 3 cut(s) 334, 1068, 1076
LweI GCATC 5 cut(s) 181, 229, 247, 322, 965
MaeI CTAG 1 cut(s) 516
MaeII ACGT 3 cut(s) 675, 952, 1030
MaeIII GTNAC 3 cut(s) 395, 646, 794
MalI GATC 5 cut(s) 285, 606, 621, 807, 906
MboI GATC 5 cut(s) 283, 604, 619, 805, 904
MboII GAAGA 8 cut(s) 57, 92, 200, 294, 614, 614, 812, 827
MhlI GDGCHC 1 cut(s) 1081
MlsI TGGCCA 1 cut(s) 638
MluCI AATT 9 cut(s) 452, 469, 682, 689, 701, 826, 852, 1000, 1045
MluNI TGGCCA 1 cut(s) 638
MlyI GAGTC 1 cut(s) 1031
MmeI TCCRAC 2 cut(s) 30, 454
MnlI CCTC 9 cut(s) 84, 140, 169, 257, 303, 428, 484, 497, 814
Mox20I TGGCCA 1 cut(s) 638
MscI TGGCCA 1 cut(s) 638
MseI TTAA 5 cut(s) 99, 552, 722, 851, 1007
MslI CAYNNNNRTG 1 cut(s) 255
Msp20I TGGCCA 1 cut(s) 638
MspI CCGG 4 cut(s) 357, 521, 698, 755
MspR9I CCNGG 1 cut(s) 422
Mva1269I GAATGC 1 cut(s) 1091
MvaI CCWGG 1 cut(s) 422
MwoI GCNNNNNNNGC 4 cut(s) 335, 569, 635, 863
NcoI CCATGG 1 cut(s) 1072
NdeII GATC 5 cut(s) 283, 604, 619, 805, 904
NlaIII CATG 7 cut(s) 320, 325, 503, 767, 866, 913, 1076
NlaIV GGNNCC 2 cut(s) 121, 1070
NmuCI GTSAC 3 cut(s) 395, 646, 794
PagI TCATGA 2 cut(s) 316, 763
PcsI WCGNNNNNNNCGW 1 cut(s) 439
PctI GAATGC 1 cut(s) 1091
PfeI GAWTC 1 cut(s) 767
PleI GAGTC 1 cut(s) 1031
PpsI GAGTC 1 cut(s) 1031
Psp124BI GAGCTC 1 cut(s) 1081
Psp6I CCWGG 1 cut(s) 420
PspGI CCWGG 1 cut(s) 420
PspN4I GGNNCC 2 cut(s) 121, 1070
PspPI GGNCC 1 cut(s) 244
RsaI GTAC 2 cut(s) 371, 1027
RsaNI GTAC 2 cut(s) 370, 1026
RseI CAYNNNNRTG 1 cut(s) 255
SacI GAGCTC 1 cut(s) 1081
SaqAI TTAA 5 cut(s) 99, 552, 722, 851, 1007
Sau3AI GATC 5 cut(s) 283, 604, 619, 805, 904
Sau96I GGNCC 1 cut(s) 244
SchI GAGTC 1 cut(s) 1031
ScrFI CCNGG 1 cut(s) 422
SduI GDGCHC 1 cut(s) 1081
SfaNI GCATC 5 cut(s) 181, 229, 247, 322, 965
SfcI CTRYAG 1 cut(s) 836
SmiMI CAYNNNNRTG 1 cut(s) 255
SmlI CTYRAG 3 cut(s) 59, 215, 931
SmoI CTYRAG 3 cut(s) 59, 215, 931
SpeI ACTAGT 1 cut(s) 515
Sse9I AATT 9 cut(s) 452, 469, 682, 689, 701, 826, 852, 1000, 1045
SspI AATATT 2 cut(s) 51, 720
SspMI CTAG 1 cut(s) 516
SstI GAGCTC 1 cut(s) 1081
StyD4I CCNGG 1 cut(s) 420
StyI CCWWGG 2 cut(s) 1020, 1072
TaaI ACNGT 3 cut(s) 212, 401, 840
TaiI ACGT 3 cut(s) 678, 955, 1033
TaqI TCGA 5 cut(s) 132, 442, 489, 948, 959
TasI AATT 9 cut(s) 452, 469, 682, 689, 701, 826, 852, 1000, 1045
TfiI GAWTC 1 cut(s) 767
Tru1I TTAA 5 cut(s) 99, 552, 722, 851, 1007
Tru9I TTAA 5 cut(s) 99, 552, 722, 851, 1007
TscAI CASTG 2 cut(s) 400, 787
TseFI GTSAC 3 cut(s) 395, 646, 794
Tsp45I GTSAC 3 cut(s) 395, 646, 794
TspDTI ATGAA 4 cut(s) 338, 780, 828, 926
TspRI CASTG 2 cut(s) 400, 787
XapI RAATTY 4 cut(s) 689, 701, 826, 1000
XspI CTAG 1 cut(s) 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.