Rroxscaffold_1G00011210

Phosphoinositide phospholipase C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010550
Physical Location & Seq
Reverse (-)
14218219 .. 14222645
4427 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00011210.1

Sequence Viewer

Length: 1413 bp
ATGGCCAACATTAATAAACAGTCTTTCAAGGTCTGCTGCTGTTTCGAAAGAATATTCAGGTTCAAGGCGGCGGAGCCGCCTGAAGATATCAAGAAAATTTTTGATAAGTACGCCGAAAATAGCATCATGTCCCTCGATGGCCTTCACAGATTTCTGGTTGAGTTTCAAGGAGAAAGTGAGGCCACCAAAGAAGATGCTCAGGCCATTTTCAACAGTCTCAAGCATCTCAACATGTTCCAAAGAAAGGGCCTCCATTTTGATGCCTTCTTTCGATATCTCTTTGGTGATCTTAACCCTCCAATTTTTTCAGAGGTGAATCATAATATGACAGCTCCATTGGCTCATTATTTCCTATACACTGGCCATAACTCATACCTGACTGGAAATCAACTCAGCAGTAACAGTAGTGTCAAACCAATTATCCAGGCACTGCAACGAGGTGTTCGAGTTATTGAATTAGATTTGTGGCCTAATTCTAAAAAGAATGGCGTCGCGGTTCTTCATGGAGGGACTTTGACTCGTCCTGTTGAACTCATCAAATGCTTGCGTGCTATTAAGGACAATGCTTTTACTGCTTCTGAATATCCTGTTGTTATAACATTTGAAGACCACTTAACTTCACCTCTTCAAGCTAAAGTGGCCAAGATGGTGACTGAAACATTTGGAGATATGCTTTACGTCCCTGACTCGGAATTTTTAATGGAATTCTCTTCACCTGAAATGTTAAAGAGAAAGATTTTGATCTCGACCAAGCCACCAGAGCATAATGAACGTCAGAGGCGCAAGGACTCTGGAGCCACTGATAAGGACAATGACAAATATGATCGGGATGAAGATGAGGAGAATGCTGATCTACAATATAAGCTGTTAATTGCAATCCATGCTGGAAAACCGAAAGGGGGAATGTTCAGAGCTAATGGAGGTTGTGGTTATGTGAAAAAACCTAAATTTTTACTTACCGGCAGTCCAGATAATGAAGTTTTTAATCCTGAAAAATCGTACCGAGTTAGGCAAATCTTGAAGGTTAAAGTTTACATGGGTGAAGGATGGCATTCAGATTTCCACCGCACACACTTTGATCTCTTCTCTCCACCGGACTTTTTTGTGAGGGTTGGAATTGCTGGAGTCCCAACGGACACACTAATGGAGGAGACTAAGACAATCGAAGACGAGTGGATTCCAGTGTGGAATCAGGAGTTCAACTTCCCATTGAGTGTTCCAGAACTAGCTGTGCTTAGAATTGAAGTTAAAGAGTACGACAGTTCTGGAAATCATGACTTTGGTGGCCAAATATGTCTGCCCATATCAGAGCTTAGAACTGGGTTCAGAGCAGTTCCTCTGCATGATAAAAAAGGGGTAAGGTATAAATCTGTGAAGCTTCTTATGAAATTTGAGTTTGAAAGTCCTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004620 GO:0004629 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006970 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0008081 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009553 GO:0009555 GO:0009556 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010600 GO:0010601 GO:0010817 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0034293 GO:0042221 GO:0042578 GO:0042742 GO:0043207 GO:0043934 GO:0044464 GO:0044703 GO:0046885 GO:0046886 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071944 GO:0090354 GO:0090355 GO:0090567 GO:0097305 GO:0097306 GO:0098542 GO:0099402 GO:1901700 GO:1901701 GO:1903046
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933
Pfam Domains
Protein Families
C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

470

Amino Acids

53.83

Weight (kDa)

6.46

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 109 - 251 3.1e-51 Phosphatidylinositol-specific phospholipase C, X domain
C2 PF00168 339 - 440 2.9e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 596
AccII CGCG 1 cut(s) 494
AciI CCGC 5 cut(s) 68, 71, 77, 494, 1066
AcoI YGGCCR 4 cut(s) 3, 361, 639, 1285
AcsI RAATTY 5 cut(s) 96, 692, 704, 947, 1388
AcuI CTGAAG 1 cut(s) 102
AcyI GRCGYC 1 cut(s) 489
AfaI GTAC 3 cut(s) 110, 1001, 1256
AfiI CCNNNNNNNGG 3 cut(s) 244, 688, 899
AflIII ACRYGT 1 cut(s) 231
AjnI CCWGG 1 cut(s) 423
AluBI AGCT 7 cut(s) 332, 632, 865, 914, 1229, 1312, 1378
AluI AGCT 7 cut(s) 332, 632, 865, 914, 1229, 1312, 1378
Alw26I GTCTC 2 cut(s) 221, 1145
AlwNI CAGNNNCTG 1 cut(s) 430
AoxI GGCC 9 cut(s) 3, 139, 180, 201, 247, 361, 467, 639, 1285
ApeKI GCWGC 1 cut(s) 36
ApoI RAATTY 5 cut(s) 96, 692, 704, 947, 1388
AseI ATTAAT 1 cut(s) 12
AspLEI GCGC 1 cut(s) 783
AspS9I GGNCC 1 cut(s) 247
AsuHPI GGTGA 6 cut(s) 296, 325, 612, 661, 705, 1052
AsuII TTCGAA 1 cut(s) 45
BalI TGGCCA 4 cut(s) 5, 363, 641, 1287
BbsI GAAGAC 2 cut(s) 612, 1173
BbvI GCAGC 1 cut(s) 23
BccI CCATC 3 cut(s) 131, 640, 1041
BciT130I CCWGG 1 cut(s) 425
BcoDI GTCTC 2 cut(s) 221, 1145
BfaI CTAG 1 cut(s) 1226
BisI GCNGC 3 cut(s) 37, 69, 77
BlsI GCNGC 3 cut(s) 38, 70, 78
Bme1390I CCNGG 1 cut(s) 425
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 2 cut(s) 75, 796
BmrFI CCNGG 1 cut(s) 425
BmrI ACTGGG 1 cut(s) 1329
BmsI GCATC 4 cut(s) 132, 184, 232, 250
BmuI ACTGGG 1 cut(s) 1329
BpiI GAAGAC 2 cut(s) 612, 1173
BpmI CTGGAG 2 cut(s) 813, 1143
Bpu10I CCTNAGC 1 cut(s) 198
Bpu14I TTCGAA 1 cut(s) 45
BpuEI CTTGAG 1 cut(s) 203
BsaBI GATNNNNATC 1 cut(s) 740
BsaHI GRCGYC 1 cut(s) 489
BsaWI WCCGGW 1 cut(s) 1093
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 688, 899
Bse118I RCCGGY 1 cut(s) 959
Bse1I ACTGG 4 cut(s) 364, 385, 1181, 1324
Bse8I GATNNNNATC 1 cut(s) 740
BseBI CCWGG 1 cut(s) 425
BseGI GGATG 2 cut(s) 835, 1052
BseJI GATNNNNATC 1 cut(s) 740
BseLI CCNNNNNNNGG 3 cut(s) 244, 688, 899
BseMII CTCAG 3 cut(s) 212, 406, 1398
BseNI ACTGG 4 cut(s) 364, 385, 1181, 1324
BseRI GAGGAG 2 cut(s) 854, 1163
BseXI GCAGC 1 cut(s) 23
Bsh1236I CGCG 1 cut(s) 494
BshFI GGCC 9 cut(s) 5, 141, 182, 203, 249, 363, 469, 641, 1287
BsiSI CCGG 2 cut(s) 960, 1094
BslFI GGGAC 4 cut(s) 115, 523, 665, 1112
BslI CCNNNNNNNGG 3 cut(s) 244, 688, 899
BsmAI GTCTC 2 cut(s) 221, 1145
BsmFI GGGAC 4 cut(s) 115, 523, 665, 1112
BsmI GAATGC 2 cut(s) 850, 1051
BsnI GGCC 9 cut(s) 5, 141, 182, 203, 249, 363, 469, 641, 1287
Bsp119I TTCGAA 1 cut(s) 45
Bsp143I GATC 5 cut(s) 286, 741, 823, 850, 1078
BspACI CCGC 5 cut(s) 68, 71, 77, 494, 1066
BspANI GGCC 9 cut(s) 5, 141, 182, 203, 249, 363, 469, 641, 1287
BspCNI CTCAG 3 cut(s) 211, 405, 1399
BspFNI CGCG 1 cut(s) 494
BspHI TCATGA 1 cut(s) 1273
BspLI GGNNCC 2 cut(s) 75, 796
BspT104I TTCGAA 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 959
BsrI ACTGG 4 cut(s) 364, 385, 1181, 1324
BssAI RCCGGY 1 cut(s) 959
BssMI GATC 5 cut(s) 286, 741, 823, 850, 1078
BssNI GRCGYC 1 cut(s) 489
Bst2UI CCWGG 1 cut(s) 425
Bst4CI ACNGT 4 cut(s) 21, 215, 404, 1262
Bst6I CTCTTC 3 cut(s) 630, 715, 1088
BstACI GRCGYC 1 cut(s) 489
BstAPI GCANNNNNTGC 1 cut(s) 881
BstBI TTCGAA 1 cut(s) 45
BstC8I GCNNGC 2 cut(s) 545, 549
BstDEI CTNAG 6 cut(s) 198, 392, 1155, 1235, 1313, 1407
BstF5I GGATG 2 cut(s) 835, 1052
BstFNI CGCG 1 cut(s) 494
BstHHI GCGC 1 cut(s) 783
BstKTI GATC 5 cut(s) 289, 744, 826, 853, 1081
BstMAI GTCTC 2 cut(s) 221, 1145
BstMBI GATC 5 cut(s) 286, 741, 823, 850, 1078
BstMWI GCNNNNNNNGC 5 cut(s) 338, 572, 638, 760, 881
BstNI CCWGG 1 cut(s) 425
BstNSI RCATGY 1 cut(s) 235
BstSCI CCNGG 1 cut(s) 423
BstUI CGCG 1 cut(s) 494
BstV1I GCAGC 1 cut(s) 23
BstV2I GAAGAC 2 cut(s) 612, 1173
BsuRI GGCC 9 cut(s) 5, 141, 182, 203, 249, 363, 469, 641, 1287
BtsCI GGATG 2 cut(s) 835, 1052
BtsI GCAGTG 1 cut(s) 428
BtsIMutI CAGTG 4 cut(s) 357, 428, 798, 1188
Cac8I GCNNGC 2 cut(s) 545, 549
CaiI CAGNNNCTG 1 cut(s) 430
CciI TCATGA 1 cut(s) 1273
CfoI GCGC 1 cut(s) 783
Cfr10I RCCGGY 1 cut(s) 959
Cfr13I GGNCC 1 cut(s) 247
CseI GACGC 1 cut(s) 478
Csp6I GTAC 3 cut(s) 109, 1000, 1255
CviAII CATG 7 cut(s) 127, 232, 503, 881, 1036, 1274, 1343
CviQI GTAC 3 cut(s) 109, 1000, 1255
DdeI CTNAG 6 cut(s) 198, 392, 1155, 1235, 1313, 1407
DpnI GATC 5 cut(s) 288, 743, 825, 852, 1080
DpnII GATC 5 cut(s) 286, 741, 823, 850, 1078
EaeI YGGCCR 4 cut(s) 3, 361, 639, 1285
Eam1104I CTCTTC 3 cut(s) 630, 715, 1088
EarI CTCTTC 3 cut(s) 630, 715, 1088
EciI GGCGGA 1 cut(s) 86
Eco32I GATATC 2 cut(s) 88, 275
Eco57I CTGAAG 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 247
EcoRI GAATTC 1 cut(s) 704
EcoRII CCWGG 1 cut(s) 423
EcoRV GATATC 2 cut(s) 88, 275
FaeI CATG 7 cut(s) 130, 235, 506, 884, 1039, 1277, 1346
FaqI GGGAC 4 cut(s) 115, 523, 665, 1112
FatI CATG 7 cut(s) 126, 231, 502, 880, 1035, 1273, 1342
Fnu4HI GCNGC 3 cut(s) 37, 69, 77
FokI GGATG 2 cut(s) 842, 1059
Fsp4HI GCNGC 3 cut(s) 37, 69, 77
FspBI CTAG 1 cut(s) 1226
GlaI GCGC 1 cut(s) 782
GluI GCNGC 3 cut(s) 37, 69, 77
GsuI CTGGAG 2 cut(s) 813, 1143
HaeIII GGCC 9 cut(s) 5, 141, 182, 203, 249, 363, 469, 641, 1287
HapII CCGG 2 cut(s) 960, 1094
HgaI GACGC 1 cut(s) 478
HhaI GCGC 1 cut(s) 783
Hin1I GRCGYC 1 cut(s) 489
Hin1II CATG 7 cut(s) 130, 235, 506, 884, 1039, 1277, 1346
Hin6I GCGC 1 cut(s) 781
HinP1I GCGC 1 cut(s) 781
HindIII AAGCTT 1 cut(s) 1376
HinfI GANTC 7 cut(s) 316, 517, 686, 788, 1125, 1177, 1189
HpaII CCGG 2 cut(s) 960, 1094
HphI GGTGA 6 cut(s) 296, 325, 612, 661, 705, 1052
Hpy166II GTNNAC 1 cut(s) 1033
Hpy188I TCNGA 8 cut(s) 310, 580, 691, 777, 911, 1057, 1309, 1328
Hpy8I GTNNAC 1 cut(s) 1033
Hpy99I CGWCG 1 cut(s) 494
HpyAV CCTTC 4 cut(s) 152, 274, 1015, 1037
HpyCH4III ACNGT 4 cut(s) 21, 215, 404, 1262
HpyCH4IV ACGT 2 cut(s) 678, 772
HpyCH4V TGCA 3 cut(s) 433, 875, 1342
HpyF10VI GCNNNNNNNGC 5 cut(s) 338, 572, 638, 760, 881
HpyF3I CTNAG 6 cut(s) 198, 392, 1155, 1235, 1313, 1407
HpySE526I ACGT 2 cut(s) 678, 772
Hsp92I GRCGYC 1 cut(s) 489
Hsp92II CATG 7 cut(s) 130, 235, 506, 884, 1039, 1277, 1346
HspAI GCGC 1 cut(s) 781
Kzo9I GATC 5 cut(s) 286, 741, 823, 850, 1078
LmnI GCTCC 3 cut(s) 73, 337, 794
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 4 cut(s) 132, 184, 232, 250
MaeI CTAG 1 cut(s) 1226
MaeII ACGT 2 cut(s) 678, 772
MaeIII GTNAC 2 cut(s) 398, 649
MalI GATC 5 cut(s) 288, 743, 825, 852, 1080
MboI GATC 5 cut(s) 286, 741, 823, 850, 1078
MboII GAAGA 9 cut(s) 95, 203, 491, 617, 617, 702, 845, 1075, 1178
MlsI TGGCCA 4 cut(s) 5, 363, 641, 1287
MluNI TGGCCA 4 cut(s) 5, 363, 641, 1287
MlyI GAGTC 4 cut(s) 511, 680, 782, 1134
MmeI TCCRAC 1 cut(s) 1093
Mox20I TGGCCA 4 cut(s) 5, 363, 641, 1287
MscI TGGCCA 4 cut(s) 5, 363, 641, 1287
MslI CAYNNNNRTG 2 cut(s) 258, 1142
Msp20I TGGCCA 4 cut(s) 5, 363, 641, 1287
MspI CCGG 2 cut(s) 960, 1094
MspR9I CCNGG 1 cut(s) 425
Mva1269I GAATGC 2 cut(s) 850, 1051
MvaI CCWGG 1 cut(s) 425
MvnI CGCG 1 cut(s) 494
MwoI GCNNNNNNNGC 5 cut(s) 338, 572, 638, 760, 881
NdeII GATC 5 cut(s) 286, 741, 823, 850, 1078
NlaIII CATG 7 cut(s) 130, 235, 506, 884, 1039, 1277, 1346
NlaIV GGNNCC 2 cut(s) 75, 796
NmuCI GTSAC 1 cut(s) 649
NspI RCATGY 1 cut(s) 235
NspV TTCGAA 1 cut(s) 45
PagI TCATGA 1 cut(s) 1273
PciI ACATGT 1 cut(s) 231
PcsI WCGNNNNNNNCGW 1 cut(s) 442
PctI GAATGC 2 cut(s) 850, 1051
PfeI GAWTC 3 cut(s) 316, 1177, 1189
PkrI GCNGC 3 cut(s) 38, 70, 78
PleI GAGTC 4 cut(s) 511, 680, 782, 1133
PpsI GAGTC 4 cut(s) 511, 680, 782, 1133
PscI ACATGT 1 cut(s) 231
PshBI ATTAAT 1 cut(s) 12
PsiI TTATAA 1 cut(s) 596
Psp6I CCWGG 1 cut(s) 423
PspGI CCWGG 1 cut(s) 423
PspN4I GGNNCC 2 cut(s) 75, 796
PspPI GGNCC 1 cut(s) 247
PstNI CAGNNNCTG 1 cut(s) 430
RsaI GTAC 3 cut(s) 110, 1001, 1256
RsaNI GTAC 3 cut(s) 109, 1000, 1255
RseI CAYNNNNRTG 2 cut(s) 258, 1142
SatI GCNGC 3 cut(s) 37, 69, 77
Sau3AI GATC 5 cut(s) 286, 741, 823, 850, 1078
Sau96I GGNCC 1 cut(s) 247
SchI GAGTC 4 cut(s) 511, 680, 782, 1134
ScrFI CCNGG 1 cut(s) 425
SfaNI GCATC 4 cut(s) 132, 184, 232, 250
SfuI TTCGAA 1 cut(s) 45
SmiMI CAYNNNNRTG 2 cut(s) 258, 1142
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
SsiI CCGC 5 cut(s) 68, 71, 77, 494, 1066
SspI AATATT 1 cut(s) 54
SspMI CTAG 1 cut(s) 1226
StyD4I CCNGG 1 cut(s) 423
TaaI ACNGT 4 cut(s) 21, 215, 404, 1262
TaiI ACGT 2 cut(s) 681, 775
TaqI TCGA 6 cut(s) 45, 135, 271, 445, 746, 1164
TauI GCSGC 2 cut(s) 71, 79
TfiI GAWTC 3 cut(s) 316, 1177, 1189
TscAI CASTG 4 cut(s) 364, 435, 805, 1188
TseFI GTSAC 1 cut(s) 649
TseI GCWGC 1 cut(s) 36
Tsp45I GTSAC 1 cut(s) 649
TspDTI ATGAA 5 cut(s) 491, 783, 846, 990, 1400
TspGWI ACGGA 1 cut(s) 1148
TspRI CASTG 4 cut(s) 364, 435, 805, 1188
VspI ATTAAT 1 cut(s) 12
XapI RAATTY 5 cut(s) 96, 692, 704, 947, 1388
XceI RCATGY 1 cut(s) 235
XspI CTAG 1 cut(s) 1226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.