Rw5G042690

Phosphoinositide phospholipase C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010550
Physical Location & Seq
Forward (+)
74667241 .. 74672937
5697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G042690.1

Sequence Viewer

Length: 1665 bp
ATGGCCAACATTAATAAACAGTCTTTCAAGGTCTGCTGCTGTTTCGAAAGAATATTCAGGTTCAAGGCGGCGGAGCCGGCTGAAGATATCAAGAAAATTTTTGATAAGTACGCCGAAAATAGCATCATGTCCCTCGATGGTCTTCACAGATTTCTGGTTGAGTTTCAAGGAGAAAGTGAGGCCACCAGAGAAGATGCTCAGGCCATTTTCAACAGTCTCAAGCATCTCAACATGTTCCAAAGAAAGGGCCTCCATTTTGATGCCTTCTTTCGATATCTCTTTGGTGATCTTAACCCTCCAATTTTTTCAGAGGTGAATCATAATATGAAAGCTCCATTGGCTCATTATTTCCTATACACTGGCCATAACTCATACCTGACTGGAAATCAACTCAGCAGTAACAGTAGTGTCAAACCAATTATCCAGGCACTGCAACGAGGTGTTCGAGTTATTGAATTAGATTTGTGGCCTAATTCTAAAAAGAATGGCGTCGCGGTTCTTCATGGAGGGACTTTGACTCGACCTGTTGAACTCATCAAATGCTTGCGTGCTATTAAGGACAATGCTTTTACTGCTTCTGAATATCCTGTTGTTATAACATTTGAAGACCACTTAACTTCACCTCTTCAAGCTAAAGTGGCCAAGATGGTGACTGAAACATTTGGAGATATGCTTTACGTCCCTGACTCGGAATTTTTAATGGAATTCTCTTCACCTGAAATGTTAAAGAGAAAGATTTTGATCTCGACCAAGCCACCAGAGCATAATGAACGTCAGAGGCGCAAGGACTCTGGAGCCACTGATAAGGACAATGACAAAGATGATCGGGATGAAGATGAGGAGAATGCTGATCTACAATATAAGCAGTTAATTGCAATCCATGCTGGAAAACCGAAAGGGGGGTTGGATATGTTGCATATTGATCCCAAGAAAGTCAGACGTCTTAGCTTGAGCGAGCAACAACTCGAACGTGCATCAAAAACTCGTGGAACAGATATTGTTAGGTTTACACAACGAAACTTGTTAAGGATATATCCCAAAGGTACACGTGTGGACTCATCAAATTATGACCCTATGCTTGGATGGAGTCATGGAGCTCAAATGGTCGCATTCAATATGCAAGGACATGATAAGCACCTTCATATTATGGAAGGAATGTTCAGAGCTAATGGAGGTTGTGGTTATGTGAAAAAACCTAAATTTTTACTTACCGGCAGTCCAGATAATGAGGTTTTTAATCCTGAAAAATCGTACCGAGTTAGGCAAATCTTGAAGGTTAAAGTTTACATGGGTGAAGGATGGCATTCAGACTTCCACCGCACGCACTTTGATCTCTTCTCTCCACCGGACTTTTTTGTGAGGGTTGGAATTGCTGGAGTACCAATGGACACACTAATGGAGGAGACTAAGACAATCGAAGACGAGTGGATTCCAGTGTGGAATCAGGAGTTCAACTTCCCATTGAGTGTTCCAGAACTAGCTGTGCTTAGAATTGAAGTTAAAGAGTACGACAGTTCTGGAAATCATGACTTTGGTGGCCAAATATGTCTGCCAATATCAGAGCTTAGAACTGGGTTCAGAGCAGTTCCTCTGCATGATAAAAAAGGGGTAAGGTATAAATCTGTGAAGCTTCTTATGAAATTTGAGTTTGAAATTCCTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004620 GO:0004629 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006970 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0008081 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009553 GO:0009555 GO:0009556 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010600 GO:0010601 GO:0010817 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0034293 GO:0042221 GO:0042578 GO:0042742 GO:0043207 GO:0043934 GO:0044464 GO:0044703 GO:0046885 GO:0046886 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071944 GO:0090354 GO:0090355 GO:0090567 GO:0097305 GO:0097306 GO:0098542 GO:0099402 GO:1901700 GO:1901701 GO:1903046
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933
Pfam Domains
Protein Families
C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

554

Amino Acids

63.62

Weight (kDa)

7.33

Isoelectric Point (pI)

41.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 109 - 251 4.9e-51 Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-Y PF00387 315 - 401 3.5e-30 Phosphatidylinositol-specific phospholipase C, Y domain
C2 PF00168 423 - 524 9.5e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 596
AatII GACGTC 1 cut(s) 943
AccII CGCG 1 cut(s) 494
AciI CCGC 4 cut(s) 68, 71, 494, 1318
AclWI GGATC 1 cut(s) 917
AcoI YGGCCR 4 cut(s) 3, 361, 639, 1537
AcsI RAATTY 6 cut(s) 96, 692, 704, 1199, 1640, 1653
AcuI CTGAAG 1 cut(s) 102
AcvI CACGTG 1 cut(s) 1049
AcyI GRCGYC 2 cut(s) 489, 940
AfaI GTAC 5 cut(s) 110, 1045, 1253, 1380, 1508
AfiI CCNNNNNNNGG 4 cut(s) 244, 688, 899, 1079
AflIII ACRYGT 3 cut(s) 231, 1046, 1048
AjnI CCWGG 1 cut(s) 423
AjuI GAANNNNNNNTTGG 4 cut(s) 320, 352, 887, 919
AloI GAACNNNNNNTCC 2 cut(s) 1142, 1174
AluBI AGCT 8 cut(s) 332, 632, 948, 1097, 1166, 1481, 1564, 1630
AluI AGCT 8 cut(s) 332, 632, 948, 1097, 1166, 1481, 1564, 1630
Alw21I GWGCWC 1 cut(s) 1099
Alw26I GTCTC 2 cut(s) 221, 1397
AlwI GGATC 1 cut(s) 917
AlwNI CAGNNNCTG 1 cut(s) 430
AoxI GGCC 8 cut(s) 3, 180, 201, 247, 361, 467, 639, 1537
ApeKI GCWGC 1 cut(s) 36
ApoI RAATTY 6 cut(s) 96, 692, 704, 1199, 1640, 1653
AseI ATTAAT 1 cut(s) 12
AspLEI GCGC 1 cut(s) 783
AspS9I GGNCC 1 cut(s) 247
AsuHPI GGTGA 6 cut(s) 296, 325, 612, 661, 705, 1304
AsuII TTCGAA 1 cut(s) 45
BaeI ACNNNNGTAYC 2 cut(s) 1035, 1068
BalI TGGCCA 4 cut(s) 5, 363, 641, 1539
BanII GRGCYC 1 cut(s) 1099
BauI CACGAG 1 cut(s) 984
BbrPI CACGTG 1 cut(s) 1049
BbsI GAAGAC 3 cut(s) 134, 612, 1425
Bbv12I GWGCWC 1 cut(s) 1099
BbvI GCAGC 1 cut(s) 23
BccI CCATC 4 cut(s) 131, 640, 1077, 1293
BciT130I CCWGG 1 cut(s) 425
BcoDI GTCTC 2 cut(s) 221, 1397
BfaI CTAG 1 cut(s) 1478
BisI GCNGC 2 cut(s) 37, 69
BlsI GCNGC 2 cut(s) 38, 70
Bme1390I CCNGG 1 cut(s) 425
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 2 cut(s) 75, 796
BmrFI CCNGG 1 cut(s) 425
BmrI ACTGGG 1 cut(s) 1581
BmsI GCATC 5 cut(s) 132, 184, 232, 250, 983
BmuI ACTGGG 1 cut(s) 1581
BpiI GAAGAC 3 cut(s) 134, 612, 1425
BpmI CTGGAG 2 cut(s) 813, 1395
Bpu10I CCTNAGC 1 cut(s) 198
Bpu14I TTCGAA 1 cut(s) 45
BpuEI CTTGAG 2 cut(s) 203, 970
BsaAI YACGTR 1 cut(s) 1049
BsaBI GATNNNNATC 1 cut(s) 740
BsaHI GRCGYC 2 cut(s) 489, 940
BsaWI WCCGGW 1 cut(s) 1345
Bsc4I CCNNNNNNNGG 4 cut(s) 244, 688, 899, 1079
Bse118I RCCGGY 2 cut(s) 76, 1211
Bse1I ACTGG 4 cut(s) 364, 385, 1433, 1576
Bse8I GATNNNNATC 1 cut(s) 740
BseBI CCWGG 1 cut(s) 425
BseGI GGATG 3 cut(s) 835, 1088, 1304
BseJI GATNNNNATC 1 cut(s) 740
BseLI CCNNNNNNNGG 4 cut(s) 244, 688, 899, 1079
BseMII CTCAG 3 cut(s) 212, 406, 1650
BseNI ACTGG 4 cut(s) 364, 385, 1433, 1576
BseRI GAGGAG 2 cut(s) 854, 1415
BseXI GCAGC 1 cut(s) 23
Bsh1236I CGCG 1 cut(s) 494
BshFI GGCC 8 cut(s) 5, 182, 203, 249, 363, 469, 641, 1539
BsiHKAI GWGCWC 1 cut(s) 1099
BsiSI CCGG 3 cut(s) 77, 1212, 1346
BslFI GGGAC 3 cut(s) 115, 523, 665
BslI CCNNNNNNNGG 4 cut(s) 244, 688, 899, 1079
BsmAI GTCTC 2 cut(s) 221, 1397
BsmFI GGGAC 3 cut(s) 115, 523, 665
BsmI GAATGC 3 cut(s) 850, 1109, 1303
BsnI GGCC 8 cut(s) 5, 182, 203, 249, 363, 469, 641, 1539
Bsp119I TTCGAA 1 cut(s) 45
Bsp1286I GDGCHC 1 cut(s) 1099
Bsp143I GATC 6 cut(s) 286, 741, 823, 850, 922, 1330
BspACI CCGC 4 cut(s) 68, 71, 494, 1318
BspANI GGCC 8 cut(s) 5, 182, 203, 249, 363, 469, 641, 1539
BspCNI CTCAG 3 cut(s) 211, 405, 1651
BspFNI CGCG 1 cut(s) 494
BspHI TCATGA 1 cut(s) 1525
BspLI GGNNCC 2 cut(s) 75, 796
BspPI GGATC 1 cut(s) 917
BspT104I TTCGAA 1 cut(s) 45
BsrFI RCCGGY 2 cut(s) 76, 1211
BsrI ACTGG 4 cut(s) 364, 385, 1433, 1576
BssAI RCCGGY 2 cut(s) 76, 1211
BssMI GATC 6 cut(s) 286, 741, 823, 850, 922, 1330
BssNI GRCGYC 2 cut(s) 489, 940
BssSI CACGAG 1 cut(s) 984
Bst2BI CACGAG 1 cut(s) 984
Bst2UI CCWGG 1 cut(s) 425
Bst4CI ACNGT 4 cut(s) 21, 215, 404, 1514
Bst6I CTCTTC 3 cut(s) 630, 715, 1340
BstACI GRCGYC 2 cut(s) 489, 940
BstAPI GCANNNNNTGC 1 cut(s) 881
BstBAI YACGTR 1 cut(s) 1049
BstBI TTCGAA 1 cut(s) 45
BstC8I GCNNGC 5 cut(s) 78, 545, 549, 956, 1322
BstDEI CTNAG 7 cut(s) 198, 392, 944, 1407, 1487, 1565, 1659
BstF5I GGATG 3 cut(s) 835, 1088, 1304
BstFNI CGCG 1 cut(s) 494
BstHHI GCGC 1 cut(s) 783
BstKTI GATC 6 cut(s) 289, 744, 826, 853, 925, 1333
BstMAI GTCTC 2 cut(s) 221, 1397
BstMBI GATC 6 cut(s) 286, 741, 823, 850, 922, 1330
BstMWI GCNNNNNNNGC 6 cut(s) 77, 338, 572, 638, 760, 881
BstNI CCWGG 1 cut(s) 425
BstNSI RCATGY 1 cut(s) 235
BstSCI CCNGG 1 cut(s) 423
BstUI CGCG 1 cut(s) 494
BstV1I GCAGC 1 cut(s) 23
BstV2I GAAGAC 3 cut(s) 134, 612, 1425
BsuRI GGCC 8 cut(s) 5, 182, 203, 249, 363, 469, 641, 1539
BtsCI GGATG 3 cut(s) 835, 1088, 1304
BtsI GCAGTG 1 cut(s) 428
BtsIMutI CAGTG 4 cut(s) 357, 428, 798, 1440
Cac8I GCNNGC 5 cut(s) 78, 545, 549, 956, 1322
CaiI CAGNNNCTG 1 cut(s) 430
CciI TCATGA 1 cut(s) 1525
CfoI GCGC 1 cut(s) 783
Cfr10I RCCGGY 2 cut(s) 76, 1211
Cfr13I GGNCC 1 cut(s) 247
CseI GACGC 1 cut(s) 478
Csp6I GTAC 5 cut(s) 109, 1044, 1252, 1379, 1507
CviAII CATG 9 cut(s) 127, 232, 503, 881, 1091, 1127, 1288, 1526, 1595
CviQI GTAC 5 cut(s) 109, 1044, 1252, 1379, 1507
DdeI CTNAG 7 cut(s) 198, 392, 944, 1407, 1487, 1565, 1659
DpnI GATC 6 cut(s) 288, 743, 825, 852, 924, 1332
DpnII GATC 6 cut(s) 286, 741, 823, 850, 922, 1330
EaeI YGGCCR 4 cut(s) 3, 361, 639, 1537
Eam1104I CTCTTC 3 cut(s) 630, 715, 1340
EarI CTCTTC 3 cut(s) 630, 715, 1340
EciI GGCGGA 1 cut(s) 86
Ecl136II GAGCTC 1 cut(s) 1097
Eco24I GRGCYC 1 cut(s) 1099
Eco32I GATATC 2 cut(s) 88, 275
Eco53kI GAGCTC 1 cut(s) 1097
Eco57I CTGAAG 1 cut(s) 102
Eco72I CACGTG 1 cut(s) 1049
EcoICRI GAGCTC 1 cut(s) 1097
EcoO109I RGGNCCY 1 cut(s) 247
EcoRI GAATTC 1 cut(s) 704
EcoRII CCWGG 1 cut(s) 423
EcoRV GATATC 2 cut(s) 88, 275
EcoT38I GRGCYC 1 cut(s) 1099
FaeI CATG 9 cut(s) 130, 235, 506, 884, 1094, 1130, 1291, 1529, 1598
FaqI GGGAC 3 cut(s) 115, 523, 665
FatI CATG 9 cut(s) 126, 231, 502, 880, 1090, 1126, 1287, 1525, 1594
Fnu4HI GCNGC 2 cut(s) 37, 69
FokI GGATG 3 cut(s) 842, 1095, 1311
FriOI GRGCYC 1 cut(s) 1099
Fsp4HI GCNGC 2 cut(s) 37, 69
FspBI CTAG 1 cut(s) 1478
GlaI GCGC 1 cut(s) 782
GluI GCNGC 2 cut(s) 37, 69
GsuI CTGGAG 2 cut(s) 813, 1395
HaeIII GGCC 8 cut(s) 5, 182, 203, 249, 363, 469, 641, 1539
HapII CCGG 3 cut(s) 77, 1212, 1346
HgaI GACGC 1 cut(s) 478
HhaI GCGC 1 cut(s) 783
Hin1I GRCGYC 2 cut(s) 489, 940
Hin1II CATG 9 cut(s) 130, 235, 506, 884, 1094, 1130, 1291, 1529, 1598
Hin6I GCGC 1 cut(s) 781
HinP1I GCGC 1 cut(s) 781
HindIII AAGCTT 1 cut(s) 1628
HinfI GANTC 8 cut(s) 316, 517, 686, 788, 1055, 1087, 1429, 1441
HpaII CCGG 3 cut(s) 77, 1212, 1346
HphI GGTGA 6 cut(s) 296, 325, 612, 661, 705, 1304
Hpy166II GTNNAC 4 cut(s) 1008, 1046, 1054, 1285
Hpy188I TCNGA 9 cut(s) 310, 580, 691, 777, 938, 1163, 1309, 1561, 1580
Hpy8I GTNNAC 4 cut(s) 1008, 1046, 1054, 1285
Hpy99I CGWCG 1 cut(s) 494
HpyAV CCTTC 5 cut(s) 274, 1145, 1148, 1267, 1289
HpyCH4III ACNGT 4 cut(s) 21, 215, 404, 1514
HpyCH4IV ACGT 5 cut(s) 678, 772, 940, 970, 1048
HpyCH4V TGCA 6 cut(s) 433, 875, 916, 974, 1120, 1594
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 338, 572, 638, 760, 881
HpyF3I CTNAG 7 cut(s) 198, 392, 944, 1407, 1487, 1565, 1659
HpySE526I ACGT 5 cut(s) 678, 772, 940, 970, 1048
Hsp92I GRCGYC 2 cut(s) 489, 940
Hsp92II CATG 9 cut(s) 130, 235, 506, 884, 1094, 1130, 1291, 1529, 1598
HspAI GCGC 1 cut(s) 781
KroI GCCGGC 1 cut(s) 76
KroNI GCCGGC 1 cut(s) 78
Kzo9I GATC 6 cut(s) 286, 741, 823, 850, 922, 1330
LmnI GCTCC 4 cut(s) 73, 337, 794, 1094
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 5 cut(s) 132, 184, 232, 250, 983
MaeI CTAG 1 cut(s) 1478
MaeII ACGT 5 cut(s) 678, 772, 940, 970, 1048
MaeIII GTNAC 2 cut(s) 398, 649
MalI GATC 6 cut(s) 288, 743, 825, 852, 924, 1332
MboI GATC 6 cut(s) 286, 741, 823, 850, 922, 1330
MhlI GDGCHC 1 cut(s) 1099
MlsI TGGCCA 4 cut(s) 5, 363, 641, 1539
MluNI TGGCCA 4 cut(s) 5, 363, 641, 1539
MlyI GAGTC 5 cut(s) 511, 680, 782, 1049, 1096
MmeI TCCRAC 2 cut(s) 885, 1345
Mox20I TGGCCA 4 cut(s) 5, 363, 641, 1539
MroNI GCCGGC 1 cut(s) 76
MscI TGGCCA 4 cut(s) 5, 363, 641, 1539
MslI CAYNNNNRTG 2 cut(s) 258, 1394
Msp20I TGGCCA 4 cut(s) 5, 363, 641, 1539
MspI CCGG 3 cut(s) 77, 1212, 1346
MspR9I CCNGG 1 cut(s) 425
Mva1269I GAATGC 3 cut(s) 850, 1109, 1303
MvaI CCWGG 1 cut(s) 425
MvnI CGCG 1 cut(s) 494
MwoI GCNNNNNNNGC 6 cut(s) 77, 338, 572, 638, 760, 881
NaeI GCCGGC 1 cut(s) 78
NdeII GATC 6 cut(s) 286, 741, 823, 850, 922, 1330
NgoMIV GCCGGC 1 cut(s) 76
NlaIII CATG 9 cut(s) 130, 235, 506, 884, 1094, 1130, 1291, 1529, 1598
NlaIV GGNNCC 2 cut(s) 75, 796
NmuCI GTSAC 1 cut(s) 649
NspI RCATGY 1 cut(s) 235
NspV TTCGAA 1 cut(s) 45
PagI TCATGA 1 cut(s) 1525
PciI ACATGT 1 cut(s) 231
PcsI WCGNNNNNNNCGW 1 cut(s) 442
PctI GAATGC 3 cut(s) 850, 1109, 1303
PdiI GCCGGC 1 cut(s) 78
PfeI GAWTC 3 cut(s) 316, 1429, 1441
PkrI GCNGC 2 cut(s) 38, 70
PleI GAGTC 5 cut(s) 511, 680, 782, 1049, 1095
PmaCI CACGTG 1 cut(s) 1049
PmlI CACGTG 1 cut(s) 1049
PpsI GAGTC 5 cut(s) 511, 680, 782, 1049, 1095
Ppu21I YACGTR 1 cut(s) 1049
PscI ACATGT 1 cut(s) 231
PshBI ATTAAT 1 cut(s) 12
PsiI TTATAA 1 cut(s) 596
Psp124BI GAGCTC 1 cut(s) 1099
Psp6I CCWGG 1 cut(s) 423
PspCI CACGTG 1 cut(s) 1049
PspGI CCWGG 1 cut(s) 423
PspN4I GGNNCC 2 cut(s) 75, 796
PspPI GGNCC 1 cut(s) 247
PstNI CAGNNNCTG 1 cut(s) 430
RsaI GTAC 5 cut(s) 110, 1045, 1253, 1380, 1508
RsaNI GTAC 5 cut(s) 109, 1044, 1252, 1379, 1507
RseI CAYNNNNRTG 2 cut(s) 258, 1394
SacI GAGCTC 1 cut(s) 1099
SatI GCNGC 2 cut(s) 37, 69
Sau3AI GATC 6 cut(s) 286, 741, 823, 850, 922, 1330
Sau96I GGNCC 1 cut(s) 247
SchI GAGTC 5 cut(s) 511, 680, 782, 1049, 1096
ScrFI CCNGG 1 cut(s) 425
SduI GDGCHC 1 cut(s) 1099
SfaNI GCATC 5 cut(s) 132, 184, 232, 250, 983
SfuI TTCGAA 1 cut(s) 45
SmiMI CAYNNNNRTG 2 cut(s) 258, 1394
SmlI CTYRAG 2 cut(s) 218, 949
SmoI CTYRAG 2 cut(s) 218, 949
SsiI CCGC 4 cut(s) 68, 71, 494, 1318
SspI AATATT 1 cut(s) 54
SspMI CTAG 1 cut(s) 1478
SstI GAGCTC 1 cut(s) 1099
StyD4I CCNGG 1 cut(s) 423
TaaI ACNGT 4 cut(s) 21, 215, 404, 1514
TaiI ACGT 5 cut(s) 681, 775, 943, 973, 1051
TaqI TCGA 8 cut(s) 45, 135, 271, 445, 520, 746, 966, 1416
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 3 cut(s) 316, 1429, 1441
TscAI CASTG 4 cut(s) 364, 435, 805, 1440
TseFI GTSAC 1 cut(s) 649
TseI GCWGC 1 cut(s) 36
Tsp45I GTSAC 1 cut(s) 649
TspDTI ATGAA 6 cut(s) 341, 491, 783, 846, 1130, 1652
TspRI CASTG 4 cut(s) 364, 435, 805, 1440
VspI ATTAAT 1 cut(s) 12
XapI RAATTY 6 cut(s) 96, 692, 704, 1199, 1640, 1653
XceI RCATGY 1 cut(s) 235
XspI CTAG 1 cut(s) 1478
ZraI GACGTC 1 cut(s) 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.