MD11G1073400.v1.1

Phosphoinositide phospholipase C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010550
Physical Location & Seq
Reverse (-)
6226415 .. 6229920
3506 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1073400.v1.1.491

Sequence Viewer

Length: 1620 bp
ATGTCTAATAAGCAGTCTTTCAATGTCTGCTTTTGCTTCCAGAGAAAATTTAGGCTCTGGATGGCAGAACCTCCTGAAGACATTAAGAACCTTTTTGATCTATACGCGGAAAATGGCACCATGACCATTGATGGCCTGCAGAAATTTCTGATTGAGTTTCAAGGAGAATCCAGTGCTACCAAGGAGGATGCTCATGCCATTTTCAACAATCTCAAGCATCTCAACATCTTTCAACGAAAGGATCTCGACCTTGATGCCTTCTTTCGATATCTCCTCGGTGACCTTAATCCTCCTCTCTCTTCAAAGGTGCACCATGACATGAATTCTCCATTGGCTCATTATTTCCTATTTACCGGCCACAACTCTTACTTAAAGGGAAATCAACTCAGCAGTAACAGCAGTGTCCGTCCGATAATTAACGCGCTGCAACAAGGTGTTAGAGTTATCGAATTAGACTTGTGGCCGAGTTCTAAGAAAAATGACGTGAAGGTTCGTCACGGAAGGACGCTTACAAGTTCGGTGGACCTCGTCAAATGTTTGCGCGCGATCAAGGACAACGCTTTTGTTGCTTCTGAGTACCCTGTTATGCTCACTGAGACATTTGGTGACATGCTGTACTCTCCCCATTCAGAATCCTTAACGGAATTCCCTTCACCGGCATCATTGAAGAGGAAAATTTTGATTTCTACCAAGCCACCGAAGTATCCTGAATCGCAGAAACCTAGAGATCAATCTGGATCCACTGATCATAAGGAAAGCGAACAAGATGATGAAGAAGATGAAGATAAAGACGAGGAGAATGCTATTCCGGAATATAAACAGTTGATTGCGATTCACGCAGGGAAGCCGAAACGTGGGTTAGATAAATGGCATATCGATCCAAATAAAGTCAGACGTCTTAGCTTGAGCGAGCAACAACTCGAAAATGAAACAAGAACTCGTGGATCCGACATTGTCAGGTTTACTCAGAGGAATTTGTTGAGGGTTTATCCAAAGGGTACACGTTTGAACTCATCAAATTATAAGCCTATGCTTGCATGGACTCATGGAGCTCAAATGGTGGCATTCAATATGCAAGGACATGGAAAGCACCTTTGGATTATGGAAGGAATGTTCAGAGCAAATGGAGGTTGCGGCTATGTGAAGAAACCTGAATTTTTGCTGGCTAGCCCAAATAATGAGGTTTTTGACCCTAATGCATCTTTACCGGTGAAAAAAACTTTGAAGGTAAAAGTATACATGGGTGACGGATGGCGTTTAGATTTCCACCGCACACACTTTGATCTATGTTCTCCACCGGACTTCTACGTGAGGGTTGGAATTGAGGGAGTCCCGAGGGACAAAACAAAAATGCTGAAAACCGAACCAATAGAGGACCAATGGGTGCCAGTTTGGAACAAGGAGTTCACCTTCCCATTGAGGGTTCCAGAGCTGGCTGTGCTTAGAGTTGAAGTCAATGAGTATGACACTCCCGCCAGACATGACTTTGGTGGCCAAACATGTCTGCCAATACGAGAGCTCAGAACTGGGATCCGAGCTGTTCCTCTGCACAGCGAAAGAGGTCAAAAGTATAGATCTGTGAGGCTTCTGATGAGATTTGAATTGACAAATGAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004620 GO:0004629 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006970 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0008081 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009553 GO:0009555 GO:0009556 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010600 GO:0010601 GO:0010817 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0034293 GO:0042221 GO:0042578 GO:0042742 GO:0043207 GO:0043934 GO:0044464 GO:0044703 GO:0046885 GO:0046886 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071944 GO:0090354 GO:0090355 GO:0090567 GO:0097305 GO:0097306 GO:0098542 GO:0099402 GO:1901700 GO:1901701 GO:1903046
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933
Pfam Domains
Protein Families
C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

540

Amino Acids

62.11

Weight (kDa)

8.63

Isoelectric Point (pI)

51.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 107 - 198 1.9e-32 Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-X PF00388 196 - 231 3.3e-06 Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-Y PF00387 299 - 386 1.8e-29 Phosphatidylinositol-specific phospholipase C, Y domain
C2 PF00168 407 - 509 2.6e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1023
AatII GACGTC 1 cut(s) 898
AccB1I GGYRCC 2 cut(s) 116, 1384
AccI GTMKAC 1 cut(s) 1236
AccII CGCG 4 cut(s) 107, 422, 543, 545
AccIII TCCGGA 1 cut(s) 808
AciI CCGC 4 cut(s) 107, 1134, 1270, 1473
AclWI GGATC 8 cut(s) 249, 732, 745, 872, 939, 952, 1525, 1538
AcoI YGGCCR 3 cut(s) 355, 461, 1492
AcsI RAATTY 7 cut(s) 47, 143, 322, 644, 675, 973, 1154
AcuI CTGAAG 1 cut(s) 96
AcyI GRCGYC 1 cut(s) 895
AfaI GTAC 3 cut(s) 578, 617, 1000
AfiI CCNNNNNNNGG 3 cut(s) 655, 854, 1420
AflIII ACRYGT 2 cut(s) 1001, 1499
AgeI ACCGGT 1 cut(s) 1207
AjiI CACGTC 1 cut(s) 484
AjuI GAANNNNNNNTTGG 4 cut(s) 314, 346, 1078, 1110
AloI GAACNNNNNNTCC 2 cut(s) 1097, 1129
AluBI AGCT 5 cut(s) 903, 1052, 1432, 1519, 1538
AluI AGCT 5 cut(s) 903, 1052, 1432, 1519, 1538
Alw21I GWGCWC 3 cut(s) 312, 1054, 1521
Alw26I GTCTC 1 cut(s) 590
Alw44I GTGCAC 1 cut(s) 308
AlwI GGATC 8 cut(s) 249, 732, 745, 872, 939, 952, 1525, 1538
Ama87I CYCGRG 1 cut(s) 1333
Aor13HI TCCGGA 1 cut(s) 808
AoxI GGCC 4 cut(s) 133, 355, 461, 1492
ApaLI GTGCAC 1 cut(s) 308
ApeKI GCWGC 1 cut(s) 424
ApoI RAATTY 7 cut(s) 47, 143, 322, 644, 675, 973, 1154
ArsI GACNNNNNNTTYG 2 cut(s) 545, 577
AsiGI ACCGGT 1 cut(s) 1207
AspLEI GCGC 3 cut(s) 424, 543, 545
AspS9I GGNCC 2 cut(s) 523, 1375
AsuHPI GGTGA 6 cut(s) 290, 617, 645, 1222, 1256, 1399
AsuNHI GCTAGC 1 cut(s) 1166
AvaI CYCGRG 1 cut(s) 1333
AvaII GGWCC 2 cut(s) 523, 1375
BaeGI GKGCMC 1 cut(s) 312
BaeI ACNNNNGTAYC 2 cut(s) 686, 719
BalI TGGCCA 1 cut(s) 1494
BamHI GGATCC 3 cut(s) 737, 944, 1530
BanI GGYRCC 2 cut(s) 116, 1384
BanII GRGCYC 2 cut(s) 1054, 1521
BarI GAAGNNNNNNTAC 2 cut(s) 493, 525
BauI CACGAG 1 cut(s) 939
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 3 cut(s) 312, 1054, 1521
BbvI GCAGC 1 cut(s) 411
BccI CCATC 3 cut(s) 55, 125, 1245
BcgI CGANNNNNNTGC 4 cut(s) 236, 270, 782, 816
BciVI GTATCC 1 cut(s) 714
BclI TGATCA 1 cut(s) 745
BcoDI GTCTC 1 cut(s) 590
BfaI CTAG 2 cut(s) 723, 1167
BfmI CTRYAG 1 cut(s) 137
BfuI GTATCC 1 cut(s) 714
BglII AGATCT 1 cut(s) 1574
BisI GCNGC 2 cut(s) 425, 1135
BlsI GCNGC 2 cut(s) 426, 1136
Bme18I GGWCC 2 cut(s) 523, 1375
BmeT110I CYCGRG 1 cut(s) 1333
BmgBI CACGTC 1 cut(s) 484
BmgT120I GGNCC 2 cut(s) 523, 1375
BmiI GGNNCC 6 cut(s) 118, 739, 946, 1386, 1425, 1532
BmrI ACTGGG 1 cut(s) 1536
BmsI GCATC 5 cut(s) 178, 226, 244, 668, 1208
BmtI GCTAGC 1 cut(s) 1170
BmuI ACTGGG 1 cut(s) 1536
BpiI GAAGAC 1 cut(s) 84
BpuEI CTTGAG 2 cut(s) 197, 925
Bsa29I ATCGAT 1 cut(s) 876
BsaAI YACGTR 1 cut(s) 1309
BsaHI GRCGYC 1 cut(s) 895
BsaJI CCNNGG 3 cut(s) 180, 274, 1334
BsaWI WCCGGW 3 cut(s) 808, 1207, 1297
Bsc4I CCNNNNNNNGG 3 cut(s) 655, 854, 1420
Bse118I RCCGGY 3 cut(s) 353, 655, 1207
Bse1I ACTGG 3 cut(s) 171, 1388, 1531
BseAI TCCGGA 1 cut(s) 808
BseCI ATCGAT 1 cut(s) 876
BseDI CCNNGG 3 cut(s) 180, 274, 1334
BseGI GGATG 3 cut(s) 66, 193, 1256
BseLI CCNNNNNNNGG 3 cut(s) 655, 854, 1420
BseMII CTCAG 5 cut(s) 400, 564, 585, 980, 1534
BseNI ACTGG 3 cut(s) 171, 1388, 1531
BsePI GCGCGC 1 cut(s) 541
BseRI GAGGAG 3 cut(s) 263, 282, 809
BseSI GKGCMC 1 cut(s) 312
BseXI GCAGC 1 cut(s) 411
BsgI GTGCAG 1 cut(s) 1532
Bsh1236I CGCG 4 cut(s) 107, 422, 543, 545
BshFI GGCC 4 cut(s) 135, 357, 463, 1494
BshNI GGYRCC 2 cut(s) 116, 1384
BshTI ACCGGT 1 cut(s) 1207
BshVI ATCGAT 1 cut(s) 876
BsiHKAI GWGCWC 3 cut(s) 312, 1054, 1521
BsiHKCI CYCGRG 1 cut(s) 1333
BsiSI CCGG 5 cut(s) 354, 656, 809, 1208, 1298
BslFI GGGAC 2 cut(s) 1316, 1352
BslI CCNNNNNNNGG 3 cut(s) 655, 854, 1420
BsmAI GTCTC 1 cut(s) 590
BsmFI GGGAC 2 cut(s) 1316, 1352
BsmI GAATGC 2 cut(s) 805, 1064
BsnI GGCC 4 cut(s) 135, 357, 463, 1494
BsoBI CYCGRG 1 cut(s) 1333
Bsp1286I GDGCHC 3 cut(s) 312, 1054, 1521
Bsp13I TCCGGA 1 cut(s) 808
BspACI CCGC 4 cut(s) 107, 1134, 1270, 1473
BspANI GGCC 4 cut(s) 135, 357, 463, 1494
BspCNI CTCAG 5 cut(s) 399, 565, 586, 979, 1533
BspDI ATCGAT 1 cut(s) 876
BspEI TCCGGA 1 cut(s) 808
BspFNI CGCG 4 cut(s) 107, 422, 543, 545
BspLI GGNNCC 6 cut(s) 118, 739, 946, 1386, 1425, 1532
BspMAI CTGCAG 1 cut(s) 141
BspOI GCTAGC 1 cut(s) 1170
BspPI GGATC 8 cut(s) 249, 732, 745, 872, 939, 952, 1525, 1538
BspT107I GGYRCC 2 cut(s) 116, 1384
BsrFI RCCGGY 3 cut(s) 353, 655, 1207
BsrI ACTGG 3 cut(s) 171, 1388, 1531
BssAI RCCGGY 3 cut(s) 353, 655, 1207
BssECI CCNNGG 3 cut(s) 180, 274, 1334
BssHII GCGCGC 1 cut(s) 541
BssNAI GTATAC 1 cut(s) 1237
BssNI GRCGYC 1 cut(s) 895
BssSI CACGAG 1 cut(s) 939
BssT1I CCWWGG 1 cut(s) 180
Bst1107I GTATAC 1 cut(s) 1237
Bst2BI CACGAG 1 cut(s) 939
Bst4CI ACNGT 1 cut(s) 822
Bst6I CTCTTC 2 cut(s) 304, 662
BstACI GRCGYC 1 cut(s) 895
BstBAI YACGTR 1 cut(s) 1309
BstC8I GCNNGC 7 cut(s) 137, 543, 911, 1035, 1164, 1168, 1434
BstDEI CTNAG 8 cut(s) 386, 471, 573, 594, 899, 966, 1442, 1520
BstEII GGTNACC 1 cut(s) 278
BstF5I GGATG 3 cut(s) 66, 193, 1256
BstFNI CGCG 4 cut(s) 107, 422, 543, 545
BstHHI GCGC 3 cut(s) 424, 543, 545
BstMAI GTCTC 1 cut(s) 590
BstMWI GCNNNNNNNGC 4 cut(s) 396, 566, 836, 1438
BstNSI RCATGY 2 cut(s) 613, 1503
BstPI GGTNACC 1 cut(s) 278
BstSFI CTRYAG 1 cut(s) 137
BstSLI GKGCMC 1 cut(s) 312
BstUI CGCG 4 cut(s) 107, 422, 543, 545
BstV1I GCAGC 1 cut(s) 411
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 5 cut(s) 241, 737, 944, 1530, 1574
BstYI RGATCY 5 cut(s) 241, 737, 944, 1530, 1574
BstZ17I GTATAC 1 cut(s) 1237
Bsu15I ATCGAT 1 cut(s) 876
BsuI GTATCC 1 cut(s) 714
BsuRI GGCC 4 cut(s) 135, 357, 463, 1494
BsuTUI ATCGAT 1 cut(s) 876
BtrI CACGTC 1 cut(s) 484
BtsCI GGATG 3 cut(s) 66, 193, 1256
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 4 cut(s) 178, 406, 591, 741
Cac8I GCNNGC 7 cut(s) 137, 543, 911, 1035, 1164, 1168, 1434
CfoI GCGC 3 cut(s) 424, 543, 545
Cfr10I RCCGGY 3 cut(s) 353, 655, 1207
Cfr13I GGNCC 2 cut(s) 523, 1375
ClaI ATCGAT 1 cut(s) 876
CseI GACGC 1 cut(s) 514
Csp6I GTAC 3 cut(s) 577, 616, 999
CspAI ACCGGT 1 cut(s) 1207
CspCI CAANNNNNGTGG 2 cut(s) 501, 536
CviQI GTAC 3 cut(s) 577, 616, 999
DdeI CTNAG 8 cut(s) 386, 471, 573, 594, 899, 966, 1442, 1520
EaeI YGGCCR 3 cut(s) 355, 461, 1492
Eam1104I CTCTTC 2 cut(s) 304, 662
EarI CTCTTC 2 cut(s) 304, 662
Ecl136II GAGCTC 2 cut(s) 1052, 1519
Eco130I CCWWGG 1 cut(s) 180
Eco24I GRGCYC 2 cut(s) 1054, 1521
Eco32I GATATC 1 cut(s) 269
Eco47I GGWCC 2 cut(s) 523, 1375
Eco53kI GAGCTC 2 cut(s) 1052, 1519
Eco57I CTGAAG 1 cut(s) 96
Eco88I CYCGRG 1 cut(s) 1333
Eco91I GGTNACC 1 cut(s) 278
EcoICRI GAGCTC 2 cut(s) 1052, 1519
EcoO65I GGTNACC 1 cut(s) 278
EcoRI GAATTC 2 cut(s) 322, 644
EcoRV GATATC 1 cut(s) 269
EcoT14I CCWWGG 1 cut(s) 180
EcoT22I ATGCAT 1 cut(s) 1201
EcoT38I GRGCYC 2 cut(s) 1054, 1521
ErhI CCWWGG 1 cut(s) 180
FaqI GGGAC 2 cut(s) 1316, 1352
FauI CCCGC 1 cut(s) 1480
FbaI TGATCA 1 cut(s) 745
FblI GTMKAC 1 cut(s) 1236
Fnu4HI GCNGC 2 cut(s) 425, 1135
FokI GGATG 3 cut(s) 73, 200, 1263
FriOI GRGCYC 2 cut(s) 1054, 1521
Fsp4HI GCNGC 2 cut(s) 425, 1135
FspBI CTAG 2 cut(s) 723, 1167
GlaI GCGC 3 cut(s) 423, 542, 544
GluI GCNGC 2 cut(s) 425, 1135
HaeIII GGCC 4 cut(s) 135, 357, 463, 1494
HapII CCGG 5 cut(s) 354, 656, 809, 1208, 1298
HgaI GACGC 1 cut(s) 514
HhaI GCGC 3 cut(s) 424, 543, 545
Hin1I GRCGYC 1 cut(s) 895
Hin6I GCGC 3 cut(s) 422, 541, 543
HinP1I GCGC 3 cut(s) 422, 541, 543
HinfI GANTC 6 cut(s) 167, 632, 710, 832, 1042, 1329
HpaII CCGG 5 cut(s) 354, 656, 809, 1208, 1298
HphI GGTGA 6 cut(s) 290, 617, 645, 1222, 1256, 1399
Hpy166II GTNNAC 6 cut(s) 310, 523, 963, 1001, 1237, 1407
Hpy188III TCNNGA 9 cut(s) 40, 58, 74, 245, 707, 735, 809, 1333, 1427
Hpy8I GTNNAC 6 cut(s) 310, 523, 963, 1001, 1237, 1407
HpyAV CCTTC 7 cut(s) 268, 481, 495, 660, 1100, 1219, 1420
HpyCH4III ACNGT 1 cut(s) 822
HpyCH4IV ACGT 5 cut(s) 483, 853, 895, 1003, 1308
HpyCH4V TGCA 7 cut(s) 139, 310, 427, 1037, 1075, 1199, 1549
HpyF10VI GCNNNNNNNGC 4 cut(s) 396, 566, 836, 1438
HpyF3I CTNAG 8 cut(s) 386, 471, 573, 594, 899, 966, 1442, 1520
HpySE526I ACGT 5 cut(s) 483, 853, 895, 1003, 1308
Hsp92I GRCGYC 1 cut(s) 895
HspAI GCGC 3 cut(s) 422, 541, 543
Kpn2I TCCGGA 1 cut(s) 808
Ksp22I TGATCA 1 cut(s) 745
LmnI GCTCC 1 cut(s) 1049
Lsp1109I GCAGC 1 cut(s) 411
LweI GCATC 5 cut(s) 178, 226, 244, 668, 1208
MaeI CTAG 2 cut(s) 723, 1167
MaeII ACGT 5 cut(s) 483, 853, 895, 1003, 1308
MaeIII GTNAC 5 cut(s) 278, 392, 494, 605, 1244
MboII GAAGA 7 cut(s) 89, 291, 679, 785, 788, 794, 1156
MflI RGATCY 5 cut(s) 241, 737, 944, 1530, 1574
MhlI GDGCHC 3 cut(s) 312, 1054, 1521
MlsI TGGCCA 1 cut(s) 1494
MluNI TGGCCA 1 cut(s) 1494
MlyI GAGTC 2 cut(s) 1036, 1338
MmeI TCCRAC 2 cut(s) 972, 1297
Mox20I TGGCCA 1 cut(s) 1494
Mph1103I ATGCAT 1 cut(s) 1201
MroI TCCGGA 1 cut(s) 808
MscI TGGCCA 1 cut(s) 1494
MseI TTAA 6 cut(s) 84, 285, 371, 417, 638, 1618
Msp20I TGGCCA 1 cut(s) 1494
MspI CCGG 5 cut(s) 354, 656, 809, 1208, 1298
Mva1269I GAATGC 2 cut(s) 805, 1064
MvnI CGCG 4 cut(s) 107, 422, 543, 545
MwoI GCNNNNNNNGC 4 cut(s) 396, 566, 836, 1438
NheI GCTAGC 1 cut(s) 1166
NlaIV GGNNCC 6 cut(s) 118, 739, 946, 1386, 1425, 1532
NmeAIII GCCGAG 1 cut(s) 489
NmuCI GTSAC 4 cut(s) 278, 494, 605, 1244
NsiI ATGCAT 1 cut(s) 1201
NspI RCATGY 2 cut(s) 613, 1503
PauI GCGCGC 1 cut(s) 541
PciI ACATGT 1 cut(s) 1499
PctI GAATGC 2 cut(s) 805, 1064
PfeI GAWTC 4 cut(s) 167, 632, 710, 832
PflFI GACNNNGTC 2 cut(s) 527, 953
PinAI ACCGGT 1 cut(s) 1207
PkrI GCNGC 2 cut(s) 426, 1136
PleI GAGTC 2 cut(s) 1036, 1337
PpsI GAGTC 2 cut(s) 1036, 1337
Ppu21I YACGTR 1 cut(s) 1309
PscI ACATGT 1 cut(s) 1499
PsiI TTATAA 1 cut(s) 1023
Psp124BI GAGCTC 2 cut(s) 1054, 1521
PspEI GGTNACC 1 cut(s) 278
PspN4I GGNNCC 6 cut(s) 118, 739, 946, 1386, 1425, 1532
PspPI GGNCC 2 cut(s) 523, 1375
PstI CTGCAG 1 cut(s) 141
PsuI RGATCY 5 cut(s) 241, 737, 944, 1530, 1574
PsyI GACNNNGTC 2 cut(s) 527, 953
PteI GCGCGC 1 cut(s) 541
RsaI GTAC 3 cut(s) 578, 617, 1000
RsaNI GTAC 3 cut(s) 577, 616, 999
SacI GAGCTC 2 cut(s) 1054, 1521
SaqAI TTAA 6 cut(s) 84, 285, 371, 417, 638, 1618
SatI GCNGC 2 cut(s) 425, 1135
Sau96I GGNCC 2 cut(s) 523, 1375
SchI GAGTC 2 cut(s) 1036, 1338
SduI GDGCHC 3 cut(s) 312, 1054, 1521
SfaNI GCATC 5 cut(s) 178, 226, 244, 668, 1208
SfcI CTRYAG 1 cut(s) 137
SinI GGWCC 2 cut(s) 523, 1375
SmlI CTYRAG 2 cut(s) 212, 904
SmoI CTYRAG 2 cut(s) 212, 904
SsiI CCGC 4 cut(s) 107, 1134, 1270, 1473
SspMI CTAG 2 cut(s) 723, 1167
SstI GAGCTC 2 cut(s) 1054, 1521
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 1 cut(s) 822
TaiI ACGT 5 cut(s) 486, 856, 898, 1006, 1311
TaqI TCGA 5 cut(s) 246, 265, 447, 876, 921
TatI WGTACW 1 cut(s) 615
TauI GCSGC 1 cut(s) 1137
TfiI GAWTC 4 cut(s) 167, 632, 710, 832
Tru1I TTAA 6 cut(s) 84, 285, 371, 417, 638, 1618
Tru9I TTAA 6 cut(s) 84, 285, 371, 417, 638, 1618
TscAI CASTG 4 cut(s) 178, 406, 598, 748
TseFI GTSAC 4 cut(s) 278, 494, 605, 1244
TseI GCWGC 1 cut(s) 424
Tsp45I GTSAC 4 cut(s) 278, 494, 605, 1244
TspDTI ATGAA 4 cut(s) 335, 786, 795, 942
TspGWI ACGGA 4 cut(s) 395, 513, 656, 1263
TspRI CASTG 4 cut(s) 178, 406, 598, 748
Tth111I GACNNNGTC 2 cut(s) 527, 953
VneI GTGCAC 1 cut(s) 308
VpaK11BI GGWCC 2 cut(s) 523, 1375
XapI RAATTY 7 cut(s) 47, 143, 322, 644, 675, 973, 1154
XceI RCATGY 2 cut(s) 613, 1503
XmiI GTMKAC 1 cut(s) 1236
XspI CTAG 2 cut(s) 723, 1167
ZraI GACGTC 1 cut(s) 896
Zsp2I ATGCAT 1 cut(s) 1201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.