FvH4_3g38970

Asparagine synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010661
Physical Location & Seq
Reverse (-)
33214185 .. 33218551
4367 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g38970.t1

Sequence Viewer

Length: 1761 bp
ATGTGTGGAATACTTGCCGTTCTGGGTTGCTCCGATGACTCTCAGGCCAAGAGAGTCAGGGTTCTCGAGCTTTCTCGCAGGTTGAAGCACCGTGGCCCAGATTGGAGTGGTCTGTACCAGCATGGTGACTGTTTCTTGTCCCATCAGAGGCTGGCTATTATTGATCCTGCTTCTGGAGATCAACCTCTGTTTAATGAAGACAAGTCCATTGTTGTCACGGTCAATGGAGAGATTTACAACCATGAAGAACTCAGGAAGCGTCTGCCGAATCACAAGTTCCGAACCGAAAGTGATTGTGATGTCATTGCTCATCTGTACGAAGAGTTTGGAGAGAACTTTGTGGACATGCTTGATGGCATGTTCTCTTTCGTGCTGCTGGATACCCGGGACAACAGCTTTATTGTTGCTCGTGATGCTATTGGAATCACTTCCCTCTACATGGGCTGGAGTCTTGATGGCTCAATTTGGATTTCATCAGAACTGAAAGGATTGAATGATGATTGTGAACACTTCAAGTCCTTTCCACCTGGTCACCTCTACTCTAGCAAAACAGGTGAACTAAAGAGGTGGTACAACCCCCCATGGTTCACAGAGTCCATTCCATCTATGCCATATGATCCTCTTGTTCTGAGGCGTGCCTTTGAAAACGCTGTGATCAAAAGGCTGATGACTGATGTGCCTTTTGGAGTTCTTCTATCTGGAGGCTTAGATTCATCTTTGGTTGCCTCTATCACTGCTCGTCACCTGGCAGGCACAAAGGCCGCCAAGCAATGGGGAGCACAACTCCATTCTTTCTGTGTTGGATTGGAGGGTTCGCCAGATTTGAAGGCTGGAAAAGAAGTTGCAGACTATTTGGGAACAGTTCACCATGAATTCCATTTTACTGTTCAGGATGGAATTGATGCCATAGAGGATGTTATATACCATGTTGAAACATATGATGTTACCACTATTCGAGCCAGCACCCCTATGTTTCTCATGTCACGTAAGATCAAGTCTTTGGGAGTCAAGATGGTGATTTCTGGTGAGGGTTCTGATGAGATTTTTGGTGGATATCTGTACTTCCACAAAGCACCCAACAAGGAAGAGTTTCACCGTGAAACTTGCAGAAAGATCAAAGCACTGCACATGTACGATTGCCTGAGGGCAAACAAATCAACATCTGCCTGGGGCTTAGAAGCTCGAGTCCCTTTCCTGGACAAAGACTTTATCAATGTTGCAATGAGCATTGATCCAGAGTTCAAGATGATAAAAAAGGACGAAGGCAGAATTGAGAAATGGGTCCTCAGGAAGGCCTTTGATGATGAGGAGAAGCCTTATCTGCCAAAGCACATCCTGTATAGACAGAAAGAACAATTCAGTGATGGTGTTGGGTACAGTTGGATTGATGGCCTGAAAGCTCATGCTGCTCTACATGTCAATGACAAGATGATGCAAAATGCTGCACATATCTTCCCACACAACACTCCCACTACAAAGGAAGGCTACTACTATAGAATGATTTTCGAGAGGTTCTTCCCACAGAACTCGGCTAGGCAAACTGTACCAGGAGGACCAAGTGTGGCCTGCAGTACAGCCACTGCTATTGAGTGGGATGCTGAGTGGTCTAAGAATCTTGACCCATCTGGCAGGGCTGCCCTAGGAGTCCACAACTCGGCTTATGAGAATCAGGCCGCTGTTCCGAGTGGACTAGGGCCTGAAATCATTGAAAATGTTCCCCACATGAAAGTTGGTACCCAAGGAGTTGCAATTGCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00250 ko01100 ko01110 map00250 map01100 map01110
Pfam Domains
Protein Families
Asn_synthase GATase_7

Protein Analysis

587

Amino Acids

65.92

Weight (kDa)

6.06

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase_6 PF13522 33 - 160 5.7e-33 Glutamine amidotransferase domain
GATase_7 PF13537 48 - 165 1.1e-39 Glutamine amidotransferase domain
DUF3700 PF12481 112 - 195 1.1e-10 Aluminium induced protein
Asn_synthase PF00733 210 - 361 9e-50 Asparagine synthase
Asn_synthase PF00733 359 - 511 1.7e-33 Asparagine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 69
Acc65I GGTACC 1 cut(s) 1733
AccB1I GGYRCC 1 cut(s) 1733
AccB7I CCANNNNNTGG 1 cut(s) 771
AciI CCGC 2 cut(s) 762, 1674
AclWI GGATC 3 cut(s) 158, 611, 1226
AcsI RAATTY 1 cut(s) 872
AfaI GTAC 9 cut(s) 116, 317, 572, 1061, 1133, 1376, 1545, 1573, 1735
AfiI CCNNNNNNNGG 7 cut(s) 147, 173, 439, 771, 1195, 1291, 1654
AflIII ACRYGT 2 cut(s) 1128, 1414
AgsI TTSAA 8 cut(s) 85, 493, 514, 644, 826, 932, 1243, 1709
AjnI CCWGG 5 cut(s) 526, 744, 1166, 1194, 1546
AluBI AGCT 5 cut(s) 70, 396, 1181, 1400, 1758
AluI AGCT 5 cut(s) 70, 396, 1181, 1400, 1758
Alw21I GWGCWC 1 cut(s) 781
AlwI GGATC 3 cut(s) 158, 611, 1226
AlwNI CAGNNNCTG 2 cut(s) 151, 1580
Ama87I CYCGRG 3 cut(s) 65, 384, 1182
AoxI GGCC 8 cut(s) 45, 94, 759, 1293, 1390, 1563, 1671, 1694
ApeKI GCWGC 4 cut(s) 373, 1406, 1442, 1634
ApoI RAATTY 1 cut(s) 872
Asp700I GAANNNNTTC 3 cut(s) 427, 1089, 1713
Asp718I GGTACC 1 cut(s) 1733
AspA2I CCTAGG 1 cut(s) 1639
AspS9I GGNCC 4 cut(s) 95, 1282, 1553, 1694
AsuC2I CCSGG 2 cut(s) 385, 386
AsuHPI GGTGA 8 cut(s) 137, 524, 566, 734, 857, 1027, 1037, 1085
AvaI CYCGRG 3 cut(s) 65, 384, 1182
AvaII GGWCC 2 cut(s) 1282, 1553
AvrII CCTAGG 1 cut(s) 1639
AxyI CCTNAGG 2 cut(s) 1142, 1286
BanI GGYRCC 1 cut(s) 1733
BauI CACGAG 1 cut(s) 408
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 781
BbvI GCAGC 4 cut(s) 360, 1393, 1429, 1621
BccI CCATC 9 cut(s) 150, 347, 449, 610, 887, 1006, 1358, 1382, 1630
BceAI ACGGC 1 cut(s) 2
BciT130I CCWGG 5 cut(s) 528, 746, 1168, 1196, 1548
BciVI GTATCC 1 cut(s) 373
BclI TGATCA 1 cut(s) 654
BcnI CCSGG 2 cut(s) 385, 386
BfaI CTAG 5 cut(s) 543, 1533, 1640, 1691, 1759
BfmI CTRYAG 2 cut(s) 1492, 1567
BfuAI ACCTGC 1 cut(s) 69
BfuI GTATCC 1 cut(s) 373
BisI GCNGC 6 cut(s) 374, 762, 1407, 1443, 1635, 1674
BlnI CCTAGG 1 cut(s) 1639
BlsI GCNGC 6 cut(s) 375, 763, 1408, 1444, 1636, 1675
Bme1390I CCNGG 7 cut(s) 385, 386, 528, 746, 1168, 1196, 1548
Bme18I GGWCC 2 cut(s) 1282, 1553
BmeT110I CYCGRG 3 cut(s) 65, 384, 1182
BmgT120I GGNCC 4 cut(s) 95, 1282, 1553, 1694
BmiI GGNNCC 2 cut(s) 1283, 1735
BmrFI CCNGG 7 cut(s) 385, 386, 528, 746, 1168, 1196, 1548
BmsI GCATC 4 cut(s) 403, 892, 1422, 1585
BpiI GAAGAC 1 cut(s) 204
BplI GAGNNNNNCTC 2 cut(s) 768, 800
BpmI CTGGAG 3 cut(s) 195, 466, 720
BpuMI CCSGG 2 cut(s) 385, 386
BsaAI YACGTR 1 cut(s) 986
BsaJI CCNNGG 6 cut(s) 91, 384, 581, 1167, 1639, 1738
Bsc4I CCNNNNNNNGG 7 cut(s) 147, 173, 439, 771, 1195, 1291, 1654
Bse21I CCTNAGG 2 cut(s) 1142, 1286
Bse3DI GCAATG 3 cut(s) 303, 776, 1227
BseBI CCWGG 5 cut(s) 528, 746, 1168, 1196, 1548
BseDI CCNNGG 6 cut(s) 91, 384, 581, 1167, 1639, 1738
BseGI GGATG 4 cut(s) 898, 919, 1332, 1600
BseLI CCNNNNNNNGG 7 cut(s) 147, 173, 439, 771, 1195, 1291, 1654
BseMI GCAATG 3 cut(s) 303, 776, 1227
BseMII CTCAG 6 cut(s) 56, 265, 620, 1133, 1300, 1590
BseRI GAGGAG 1 cut(s) 1322
BseXI GCAGC 4 cut(s) 360, 1393, 1429, 1621
BsgI GTGCAG 2 cut(s) 1109, 1428
BshFI GGCC 8 cut(s) 47, 96, 761, 1295, 1392, 1565, 1673, 1696
BshNI GGYRCC 1 cut(s) 1733
BsiHKAI GWGCWC 1 cut(s) 781
BsiHKCI CYCGRG 3 cut(s) 65, 384, 1182
BsiSI CCGG 1 cut(s) 385
BslFI GGGAC 3 cut(s) 124, 401, 1172
BslI CCNNNNNNNGG 7 cut(s) 147, 173, 439, 771, 1195, 1291, 1654
BsmFI GGGAC 3 cut(s) 124, 401, 1172
BsnI GGCC 8 cut(s) 47, 96, 761, 1295, 1392, 1565, 1673, 1696
BsoBI CYCGRG 3 cut(s) 65, 384, 1182
Bsp1286I GDGCHC 1 cut(s) 781
Bsp143I GATC 7 cut(s) 163, 178, 616, 654, 990, 1113, 1231
Bsp19I CCATGG 1 cut(s) 581
BspACI CCGC 2 cut(s) 762, 1674
BspANI GGCC 8 cut(s) 47, 96, 761, 1295, 1392, 1565, 1673, 1696
BspCNI CTCAG 6 cut(s) 55, 264, 621, 1134, 1299, 1591
BspLI GGNNCC 2 cut(s) 1283, 1735
BspMAI CTGCAG 1 cut(s) 1571
BspMI ACCTGC 1 cut(s) 69
BspPI GGATC 3 cut(s) 158, 611, 1226
BspT107I GGYRCC 1 cut(s) 1733
BsrDI GCAATG 3 cut(s) 303, 776, 1227
BssECI CCNNGG 6 cut(s) 91, 384, 581, 1167, 1639, 1738
BssMI GATC 7 cut(s) 163, 178, 616, 654, 990, 1113, 1231
BssSI CACGAG 1 cut(s) 408
BssT1I CCWWGG 3 cut(s) 581, 1639, 1738
Bst2BI CACGAG 1 cut(s) 408
Bst2UI CCWGG 5 cut(s) 528, 746, 1168, 1196, 1548
Bst4CI ACNGT 8 cut(s) 92, 131, 220, 862, 886, 1097, 1379, 1543
Bst6I CTCTTC 2 cut(s) 315, 1080
BstBAI YACGTR 1 cut(s) 986
BstC8I GCNNGC 6 cut(s) 153, 636, 751, 961, 1567, 1756
BstDEI CTNAG 9 cut(s) 42, 251, 629, 706, 1142, 1174, 1286, 1599, 1608
BstDSI CCRYGG 2 cut(s) 91, 581
BstEII GGTNACC 1 cut(s) 530
BstENI CCTNNNNNAGG 1 cut(s) 1289
BstF5I GGATG 4 cut(s) 898, 919, 1332, 1600
BstKTI GATC 7 cut(s) 166, 181, 619, 657, 993, 1116, 1234
BstMBI GATC 7 cut(s) 163, 178, 616, 654, 990, 1113, 1231
BstMWI GCNNNNNNNGC 3 cut(s) 413, 1321, 1406
BstNI CCWGG 5 cut(s) 528, 746, 1168, 1196, 1548
BstNSI RCATGY 4 cut(s) 349, 361, 1132, 1418
BstPI GGTNACC 1 cut(s) 530
BstSCI CCNGG 7 cut(s) 383, 384, 526, 744, 1166, 1194, 1546
BstSFI CTRYAG 2 cut(s) 1492, 1567
BstV1I GCAGC 4 cut(s) 360, 1393, 1429, 1621
BstV2I GAAGAC 1 cut(s) 204
Bsu36I CCTNAGG 2 cut(s) 1142, 1286
BsuI GTATCC 1 cut(s) 373
BsuRI GGCC 8 cut(s) 47, 96, 761, 1295, 1392, 1565, 1673, 1696
BtgI CCRYGG 2 cut(s) 91, 581
BtsCI GGATG 4 cut(s) 898, 919, 1332, 1600
BtsI GCAGTG 3 cut(s) 732, 1121, 1578
BtsIMutI CAGTG 4 cut(s) 732, 1121, 1366, 1578
BveI ACCTGC 1 cut(s) 69
Cac8I GCNNGC 6 cut(s) 153, 636, 751, 961, 1567, 1756
CaiI CAGNNNCTG 2 cut(s) 151, 1580
Cfr13I GGNCC 4 cut(s) 95, 1282, 1553, 1694
Cfr9I CCCGGG 1 cut(s) 384
CseI GACGC 1 cut(s) 248
CsiI ACCWGGT 1 cut(s) 526
Csp6I GTAC 9 cut(s) 115, 316, 571, 1060, 1132, 1375, 1544, 1572, 1734
CspCI CAANNNNNGTGG 2 cut(s) 1567, 1602
CviQI GTAC 9 cut(s) 115, 316, 571, 1060, 1132, 1375, 1544, 1572, 1734
DdeI CTNAG 9 cut(s) 42, 251, 629, 706, 1142, 1174, 1286, 1599, 1608
DpnI GATC 7 cut(s) 165, 180, 618, 656, 992, 1115, 1233
DpnII GATC 7 cut(s) 163, 178, 616, 654, 990, 1113, 1231
Eam1104I CTCTTC 2 cut(s) 315, 1080
EarI CTCTTC 2 cut(s) 315, 1080
Eco130I CCWWGG 3 cut(s) 581, 1639, 1738
Eco147I AGGCCT 1 cut(s) 1295
Eco32I GATATC 1 cut(s) 1055
Eco47I GGWCC 2 cut(s) 1282, 1553
Eco81I CCTNAGG 2 cut(s) 1142, 1286
Eco88I CYCGRG 3 cut(s) 65, 384, 1182
Eco91I GGTNACC 1 cut(s) 530
EcoNI CCTNNNNNAGG 1 cut(s) 1289
EcoO109I RGGNCCY 2 cut(s) 1282, 1694
EcoO65I GGTNACC 1 cut(s) 530
EcoRI GAATTC 1 cut(s) 872
EcoRII CCWGG 5 cut(s) 526, 744, 1166, 1194, 1546
EcoRV GATATC 1 cut(s) 1055
EcoT14I CCWWGG 3 cut(s) 581, 1639, 1738
ErhI CCWWGG 3 cut(s) 581, 1639, 1738
FaqI GGGAC 3 cut(s) 124, 401, 1172
FauNDI CATATG 2 cut(s) 613, 937
FbaI TGATCA 1 cut(s) 654
Fnu4HI GCNGC 6 cut(s) 374, 762, 1407, 1443, 1635, 1674
FokI GGATG 4 cut(s) 905, 926, 1319, 1607
Fsp4HI GCNGC 6 cut(s) 374, 762, 1407, 1443, 1635, 1674
FspBI CTAG 5 cut(s) 543, 1533, 1640, 1691, 1759
GluI GCNGC 6 cut(s) 374, 762, 1407, 1443, 1635, 1674
GsuI CTGGAG 3 cut(s) 195, 466, 720
HaeIII GGCC 8 cut(s) 47, 96, 761, 1295, 1392, 1565, 1673, 1696
HapII CCGG 1 cut(s) 385
HgaI GACGC 1 cut(s) 248
HpaII CCGG 1 cut(s) 385
HphI GGTGA 8 cut(s) 137, 524, 566, 734, 857, 1027, 1037, 1085
Hpy166II GTNNAC 7 cut(s) 343, 506, 557, 588, 865, 1648, 1688
Hpy188I TCNGA 7 cut(s) 34, 147, 281, 478, 630, 1036, 1683
Hpy8I GTNNAC 7 cut(s) 343, 506, 557, 588, 865, 1648, 1688
HpyAV CCTTC 4 cut(s) 820, 1256, 1285, 1475
HpyCH4III ACNGT 8 cut(s) 92, 131, 220, 862, 886, 1097, 1379, 1543
HpyCH4IV ACGT 1 cut(s) 985
HpyCH4V TGCA 9 cut(s) 845, 1107, 1126, 1220, 1435, 1445, 1569, 1748, 1754
HpyF10VI GCNNNNNNNGC 3 cut(s) 413, 1321, 1406
HpyF3I CTNAG 9 cut(s) 42, 251, 629, 706, 1142, 1174, 1286, 1599, 1608
HpySE526I ACGT 1 cut(s) 985
KpnI GGTACC 1 cut(s) 1737
Ksp22I TGATCA 1 cut(s) 654
Kzo9I GATC 7 cut(s) 163, 178, 616, 654, 990, 1113, 1231
LmnI GCTCC 2 cut(s) 35, 776
Lsp1109I GCAGC 4 cut(s) 360, 1393, 1429, 1621
LweI GCATC 4 cut(s) 403, 892, 1422, 1585
MabI ACCWGGT 1 cut(s) 526
MaeI CTAG 5 cut(s) 543, 1533, 1640, 1691, 1759
MaeII ACGT 1 cut(s) 985
MaeIII GTNAC 6 cut(s) 125, 214, 530, 740, 943, 981
MalI GATC 7 cut(s) 165, 180, 618, 656, 992, 1115, 1233
MboI GATC 7 cut(s) 163, 178, 616, 654, 990, 1113, 1231
MboII GAAGA 7 cut(s) 209, 257, 332, 683, 1097, 1444, 1507
MfeI CAATTG 1 cut(s) 1749
MhlI GDGCHC 1 cut(s) 781
MluCI AATT 6 cut(s) 462, 872, 897, 1269, 1355, 1749
MlyI GAGTC 7 cut(s) 32, 63, 457, 602, 1014, 1194, 1653
MmeI TCCRAC 2 cut(s) 781, 1361
MroXI GAANNNNTTC 3 cut(s) 427, 1089, 1713
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 2 cut(s) 968, 1419
MspA1I CMGCKG 1 cut(s) 1676
MspI CCGG 1 cut(s) 385
MspR9I CCNGG 7 cut(s) 385, 386, 528, 746, 1168, 1196, 1548
MunI CAATTG 1 cut(s) 1749
MvaI CCWGG 5 cut(s) 528, 746, 1168, 1196, 1548
MwoI GCNNNNNNNGC 3 cut(s) 413, 1321, 1406
NciI CCSGG 2 cut(s) 385, 386
NcoI CCATGG 1 cut(s) 581
NdeI CATATG 2 cut(s) 613, 937
NdeII GATC 7 cut(s) 163, 178, 616, 654, 990, 1113, 1231
NlaIV GGNNCC 2 cut(s) 1283, 1735
NmeAIII GCCGAG 2 cut(s) 1508, 1634
NmuCI GTSAC 5 cut(s) 125, 214, 530, 740, 981
NspI RCATGY 4 cut(s) 349, 361, 1132, 1418
PaeR7I CTCGAG 2 cut(s) 65, 1182
PceI AGGCCT 1 cut(s) 1295
PciI ACATGT 2 cut(s) 1128, 1414
PdmI GAANNNNTTC 3 cut(s) 427, 1089, 1713
PfeI GAWTC 5 cut(s) 268, 423, 710, 1612, 1666
PflMI CCANNNNNTGG 1 cut(s) 771
PfoI TCCNGGA 1 cut(s) 1194
PkrI GCNGC 6 cut(s) 375, 763, 1408, 1444, 1636, 1675
PleI GAGTC 7 cut(s) 32, 62, 456, 601, 1013, 1193, 1652
PpsI GAGTC 7 cut(s) 32, 62, 456, 601, 1013, 1193, 1652
Ppu21I YACGTR 1 cut(s) 986
PpuMI RGGWCCY 1 cut(s) 1282
PscI ACATGT 2 cut(s) 1128, 1414
Psp5II RGGWCCY 1 cut(s) 1282
Psp6I CCWGG 5 cut(s) 526, 744, 1166, 1194, 1546
PspEI GGTNACC 1 cut(s) 530
PspGI CCWGG 5 cut(s) 526, 744, 1166, 1194, 1546
PspN4I GGNNCC 2 cut(s) 1283, 1735
PspPI GGNCC 4 cut(s) 95, 1282, 1553, 1694
PspPPI RGGWCCY 1 cut(s) 1282
PspXI VCTCGAGB 1 cut(s) 1182
PstI CTGCAG 1 cut(s) 1571
PstNI CAGNNNCTG 2 cut(s) 151, 1580
RsaI GTAC 9 cut(s) 116, 317, 572, 1061, 1133, 1376, 1545, 1573, 1735
RsaNI GTAC 9 cut(s) 115, 316, 571, 1060, 1132, 1375, 1544, 1572, 1734
RseI CAYNNNNRTG 2 cut(s) 968, 1419
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 6 cut(s) 374, 762, 1407, 1443, 1635, 1674
Sau3AI GATC 7 cut(s) 163, 178, 616, 654, 990, 1113, 1231
Sau96I GGNCC 4 cut(s) 95, 1282, 1553, 1694
SchI GAGTC 7 cut(s) 32, 63, 457, 602, 1014, 1194, 1653
ScrFI CCNGG 7 cut(s) 385, 386, 528, 746, 1168, 1196, 1548
SduI GDGCHC 1 cut(s) 781
SexAI ACCWGGT 1 cut(s) 526
SfaNI GCATC 4 cut(s) 403, 892, 1422, 1585
SfcI CTRYAG 2 cut(s) 1492, 1567
Sfr274I CTCGAG 2 cut(s) 65, 1182
SinI GGWCC 2 cut(s) 1282, 1553
SlaI CTCGAG 2 cut(s) 65, 1182
SmaI CCCGGG 1 cut(s) 386
SmiMI CAYNNNNRTG 2 cut(s) 968, 1419
SmlI CTYRAG 2 cut(s) 65, 1182
SmoI CTYRAG 2 cut(s) 65, 1182
Sse9I AATT 6 cut(s) 462, 872, 897, 1269, 1355, 1749
SseBI AGGCCT 1 cut(s) 1295
SsiI CCGC 2 cut(s) 762, 1674
SspMI CTAG 5 cut(s) 543, 1533, 1640, 1691, 1759
StuI AGGCCT 1 cut(s) 1295
StyD4I CCNGG 7 cut(s) 383, 384, 526, 744, 1166, 1194, 1546
StyI CCWWGG 3 cut(s) 581, 1639, 1738
TaaI ACNGT 8 cut(s) 92, 131, 220, 862, 886, 1097, 1379, 1543
TaiI ACGT 1 cut(s) 988
TaqI TCGA 4 cut(s) 66, 955, 1183, 1506
TasI AATT 6 cut(s) 462, 872, 897, 1269, 1355, 1749
TatI WGTACW 2 cut(s) 1059, 1571
TauI GCSGC 2 cut(s) 764, 1676
TfiI GAWTC 5 cut(s) 268, 423, 710, 1612, 1666
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 4 cut(s) 739, 1128, 1366, 1585
TseFI GTSAC 5 cut(s) 125, 214, 530, 740, 981
TseI GCWGC 4 cut(s) 373, 1406, 1442, 1634
Tsp45I GTSAC 5 cut(s) 125, 214, 530, 740, 981
TspDTI ATGAA 6 cut(s) 210, 258, 462, 702, 885, 1739
TspMI CCCGGG 1 cut(s) 384
TspRI CASTG 4 cut(s) 739, 1128, 1366, 1585
Van91I CCANNNNNTGG 1 cut(s) 771
VpaK11BI GGWCC 2 cut(s) 1282, 1553
XagI CCTNNNNNAGG 1 cut(s) 1289
XapI RAATTY 1 cut(s) 872
XceI RCATGY 4 cut(s) 349, 361, 1132, 1418
XcmI CCANNNNNNNNNTGG 1 cut(s) 1727
XhoI CTCGAG 2 cut(s) 65, 1182
XmaI CCCGGG 1 cut(s) 384
XmaJI CCTAGG 1 cut(s) 1639
XmnI GAANNNNTTC 3 cut(s) 427, 1089, 1713
XspI CTAG 5 cut(s) 543, 1533, 1640, 1691, 1759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.