MD03G1066000.v1.1

Asparagine synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010661
Physical Location & Seq
Forward (+)
5277766 .. 5282454
4689 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1066000.v1.1.491

Sequence Viewer

Length: 1761 bp
ATGTGTGGAATTCTTGCTGTTCTTGGTTGCTCTGATGACTCGCAGGCCAAGAGGGTCCGAGTTCTTGAGCTTTCGCGCAGATTGAAGCATCGTGGACCTGACTGGAGTGGGCTGTACCAGCATGGAGATTGTTTCTTGGCTCATCAGAGGTTGGCCATCATTGACCCTGCCTCTGGTGATCAACCTCTCTACAACGAAGACAAGTCAATTGTTGTCACGGTGAATGGAGAGATTTACAACCATGAAGAGCTGAGGAGCCGTCTGCCGAATCACAAGTTCCGAACCGGCAGCGATTGTGATGTCATTGCCCATCTGTATGAAGAGTATGGGGAGAATTTCGTTGATATGCTGGACGGCATGTTTTCGTTTGTGCTGCTGGATACCCGCGACAACAGCTTCATCGTTGCTCGTGATGCTGTCGGAATCACTTCCCTCTATATCGGCTGGAGTCTTGATGGCTCGGTTTGGATATCATCGGAACTGAAAGGGTTGAATGATGATTGCGAACACTTTGAGAGCTTTCCACCCGGTCATCTGTACTCGAGTAAAGAAGGTGAACTTAAGAGGTGGTACAACCCTACTTGGTTCTCGGAGACCATTCCATCAGGGCCATATGATCCGCTTGTTCTGAGGCGCGCCTTTGAAACTGCTGTGATCAAAAGGCTAATGACTGATGTGCCTTTCGGAGTTCTTCTCTCCGGAGGTCTGGATTCATCTTTGGTCGCCTCTATCACTGCTCGTCACTTGGCAGGCACAAAGGCGGCCAAGCAGTGGGGTGCACAGCTCCATTCCTTCTGTGTTGGACTCGAGGGCTCACCCGATCTGAAGGCTGCAAAAGAAGTTGCAGATTATCTGGGAACCGTTCACCACGAGTTTCATTTTACAGTTCAGGATGGAATTGACGCGATCGAAGATGTCATATACCACATAGAAACATATGATGTTACCACTATCCGAGCAAGTACCCCTATGTTTCTCATGTCGCGTAAGATCAAGTCCTTGGGGGTTAAGATGGTGATTTCTGGCGAGGGATCTGATGAAATTTTCGGTGGATACTTGTACTTCCACAAGGCACCCAACAAAGAAGAGTTCCACCGCGAAACCTGCAGAAAGATCAAGGCACTGCACATGTATGATTGCCTCAGGGCAAACAAATCAACATCTGCCTGGGGCTTAGAAGCCCGAGTCCCATTCTTGGACAAGGATTTTATCAAAGTTGCAATGGATATTGATCCCGAGTTTAAAATGATCAAAAAGGACCAAGGAAGAATTGAGAAATGGGTCCTCCGAAAGGCGTTTGACGATGAGGAGCAACCTTACTTGCCAAAGCACATTCTGTATAGGCAGAAGGAGCAGTTCAGCGACGGGGTTGGCTATAGTTGGATTGACGGCCTCAAATCCCATGCTGCTCTCCATGTCACTGATAAGATGATGATGAATGCTGAACGCATCTTCCCCCACAACACTCCGAATACAAAGGAAGCCTACTACTACCGAATGATTTTTGAAAGGTTCTTCCCACAGAACTCGGCCAGGAACACGGTGCCTGGAGGACCGAGTGTAGCCTGCAGCACAGCCACAGCTGTGGCATGGGATGCTGAATGGTCCAAAAATCTTGACCCATCTGGCAGGGCTATATTTGAAGTGCACCAACAAGCTTATGACAAGCAGGGTGCACCGTTGACTAGCGGAATTCCAGAGATCATCGACAATGTACCTCAGATGAAAGCGAGCACCGTGGGGGTTGCGATCCGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00250 ko01100 ko01110 map00250 map01100 map01110
Pfam Domains
Protein Families
Asn_synthase GATase_7

Protein Analysis

587

Amino Acids

66.1

Weight (kDa)

5.95

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase_6 PF13522 33 - 160 3.6e-33 Glutamine amidotransferase domain
GATase_7 PF13537 48 - 165 2.7e-39 Glutamine amidotransferase domain
DUF3700 PF12481 112 - 195 7.4e-11 Aluminium induced protein
Asn_synthase PF00733 210 - 361 7e-51 Asparagine synthase
Asn_synthase PF00733 359 - 511 3.4e-32 Asparagine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1112
AccB1I GGYRCC 2 cut(s) 1072, 1543
AccB7I CCANNNNNTGG 1 cut(s) 771
AccII CGCG 6 cut(s) 76, 387, 636, 905, 985, 1098
AccIII TCCGGA 1 cut(s) 698
AciI CCGC 6 cut(s) 385, 620, 761, 1096, 1689, 1753
AclWI GGATC 4 cut(s) 611, 1039, 1226, 1744
AcoI YGGCCR 3 cut(s) 153, 762, 1530
AcsI RAATTY 4 cut(s) 9, 334, 1041, 1692
AcuI CTGAAG 1 cut(s) 845
AfaI GTAC 6 cut(s) 116, 539, 572, 964, 1061, 1716
AfiI CCNNNNNNNGG 4 cut(s) 173, 771, 1195, 1291
AflII CTTAAG 1 cut(s) 560
AflIII ACRYGT 1 cut(s) 1128
AgsI TTSAA 5 cut(s) 85, 493, 644, 1508, 1643
AjnI CCWGG 3 cut(s) 1166, 1532, 1546
AleI CACNNNNGTG 1 cut(s) 1583
AloI GAACNNNNNNTCC 2 cut(s) 1437, 1469
AluBI AGCT 7 cut(s) 70, 250, 396, 519, 784, 1583, 1658
AluI AGCT 7 cut(s) 70, 250, 396, 519, 784, 1583, 1658
Alw21I GWGCWC 4 cut(s) 781, 1650, 1678, 1736
Alw26I GTCTC 1 cut(s) 587
Alw44I GTGCAC 3 cut(s) 777, 1646, 1674
AlwI GGATC 4 cut(s) 611, 1039, 1226, 1744
Ama87I CYCGRG 4 cut(s) 541, 806, 1182, 1235
Aor13HI TCCGGA 1 cut(s) 698
AoxI GGCC 6 cut(s) 45, 153, 608, 762, 1390, 1530
ApaLI GTGCAC 3 cut(s) 777, 1646, 1674
ApeKI GCWGC 5 cut(s) 288, 373, 830, 1406, 1569
ApoI RAATTY 4 cut(s) 9, 334, 1041, 1692
AscI GGCGCGCC 1 cut(s) 634
Asp700I GAANNNNTTC 1 cut(s) 427
AspLEI GCGC 3 cut(s) 78, 636, 638
AspS9I GGNCC 7 cut(s) 55, 95, 608, 1258, 1282, 1553, 1605
AsuC2I CCSGG 1 cut(s) 528
AsuHPI GGTGA 6 cut(s) 188, 232, 566, 807, 857, 1027
AvaI CYCGRG 4 cut(s) 541, 806, 1182, 1235
AvaII GGWCC 6 cut(s) 55, 95, 1258, 1282, 1553, 1605
AxyI CCTNAGG 1 cut(s) 1142
BaeGI GKGCMC 3 cut(s) 781, 1650, 1678
BalI TGGCCA 1 cut(s) 155
BanI GGYRCC 2 cut(s) 1072, 1543
BanII GRGCYC 1 cut(s) 815
BauI CACGAG 2 cut(s) 408, 869
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 4 cut(s) 781, 1650, 1678, 1736
BbvCI CCTCAGC 1 cut(s) 251
BbvI GCAGC 5 cut(s) 300, 360, 817, 1393, 1581
BccI CCATC 7 cut(s) 164, 318, 449, 610, 887, 1006, 1630
BceAI ACGGC 3 cut(s) 243, 370, 1405
BciT130I CCWGG 3 cut(s) 1168, 1534, 1548
BciVI GTATCC 2 cut(s) 373, 1046
BclI TGATCA 3 cut(s) 178, 654, 1248
BcnI CCSGG 1 cut(s) 528
BcoDI GTCTC 1 cut(s) 587
BfaI CTAG 1 cut(s) 1686
BfmI CTRYAG 3 cut(s) 1105, 1375, 1567
BfrI CTTAAG 1 cut(s) 560
BfuAI ACCTGC 1 cut(s) 1112
BfuI GTATCC 2 cut(s) 373, 1046
BisI GCNGC 6 cut(s) 289, 374, 762, 831, 1407, 1570
BlsI GCNGC 6 cut(s) 290, 375, 763, 832, 1408, 1571
Bme1390I CCNGG 4 cut(s) 528, 1168, 1534, 1548
Bme18I GGWCC 6 cut(s) 55, 95, 1258, 1282, 1553, 1605
BmeT110I CYCGRG 4 cut(s) 541, 806, 1182, 1235
BmgT120I GGNCC 7 cut(s) 55, 95, 608, 1258, 1282, 1553, 1605
BmiI GGNNCC 6 cut(s) 56, 257, 859, 1074, 1283, 1545
BmrFI CCNGG 4 cut(s) 528, 1168, 1534, 1548
BmsI GCATC 4 cut(s) 97, 403, 1458, 1585
BpiI GAAGAC 1 cut(s) 204
BplI GAGNNNNNCTC 2 cut(s) 678, 710
BpmI CTGGAG 3 cut(s) 124, 466, 1569
Bpu10I CCTNAGC 1 cut(s) 251
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 528
BsaBI GATNNNNATC 1 cut(s) 1230
BsaI GGTCTC 1 cut(s) 587
BsaJI CCNNGG 4 cut(s) 999, 1167, 1261, 1737
BsaWI WCCGGW 1 cut(s) 698
Bsc4I CCNNNNNNNGG 4 cut(s) 173, 771, 1195, 1291
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 1 cut(s) 107
Bse21I CCTNAGG 1 cut(s) 1142
Bse3DI GCAATG 2 cut(s) 303, 1227
Bse8I GATNNNNATC 1 cut(s) 1230
BseAI TCCGGA 1 cut(s) 698
BseBI CCWGG 3 cut(s) 1168, 1534, 1548
BseDI CCNNGG 4 cut(s) 999, 1167, 1261, 1737
BseGI GGATG 2 cut(s) 898, 1600
BseJI GATNNNNATC 1 cut(s) 1230
BseLI CCNNNNNNNGG 4 cut(s) 173, 771, 1195, 1291
BseMI GCAATG 2 cut(s) 303, 1227
BseMII CTCAG 4 cut(s) 242, 620, 1156, 1733
BseNI ACTGG 1 cut(s) 107
BsePI GCGCGC 1 cut(s) 634
BseRI GAGGAG 2 cut(s) 268, 1322
BseSI GKGCMC 3 cut(s) 781, 1650, 1678
BseXI GCAGC 5 cut(s) 300, 360, 817, 1393, 1581
BsgI GTGCAG 1 cut(s) 1109
Bsh1236I CGCG 6 cut(s) 76, 387, 636, 905, 985, 1098
Bsh1285I CGRYCG 1 cut(s) 909
BshFI GGCC 6 cut(s) 47, 155, 610, 764, 1392, 1532
BshNI GGYRCC 2 cut(s) 1072, 1543
BsiEI CGRYCG 1 cut(s) 909
BsiHKAI GWGCWC 4 cut(s) 781, 1650, 1678, 1736
BsiHKCI CYCGRG 4 cut(s) 541, 806, 1182, 1235
BsiSI CCGG 3 cut(s) 285, 528, 699
BslFI GGGAC 1 cut(s) 1172
BslI CCNNNNNNNGG 4 cut(s) 173, 771, 1195, 1291
BsmAI GTCTC 1 cut(s) 587
BsmFI GGGAC 1 cut(s) 1172
BsmI GAATGC 1 cut(s) 1444
BsnI GGCC 6 cut(s) 47, 155, 610, 764, 1392, 1532
Bso31I GGTCTC 1 cut(s) 587
BsoBI CYCGRG 4 cut(s) 541, 806, 1182, 1235
Bsp1286I GDGCHC 5 cut(s) 781, 815, 1650, 1678, 1736
Bsp13I TCCGGA 1 cut(s) 698
BspACI CCGC 6 cut(s) 385, 620, 761, 1096, 1689, 1753
BspANI GGCC 6 cut(s) 47, 155, 610, 764, 1392, 1532
BspCNI CTCAG 4 cut(s) 243, 621, 1155, 1732
BspEI TCCGGA 1 cut(s) 698
BspFNI CGCG 6 cut(s) 76, 387, 636, 905, 985, 1098
BspLI GGNNCC 6 cut(s) 56, 257, 859, 1074, 1283, 1545
BspMAI CTGCAG 2 cut(s) 1109, 1571
BspMI ACCTGC 1 cut(s) 1112
BspPI GGATC 4 cut(s) 611, 1039, 1226, 1744
BspQI GCTCTTC 1 cut(s) 240
BspT107I GGYRCC 2 cut(s) 1072, 1543
BspTI CTTAAG 1 cut(s) 560
BspTNI GGTCTC 1 cut(s) 587
BsrDI GCAATG 2 cut(s) 303, 1227
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 1 cut(s) 107
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 4 cut(s) 999, 1167, 1261, 1737
BssHII GCGCGC 1 cut(s) 634
BssSI CACGAG 2 cut(s) 408, 869
BssT1I CCWWGG 2 cut(s) 999, 1261
Bst2BI CACGAG 2 cut(s) 408, 869
Bst2UI CCWGG 3 cut(s) 1168, 1534, 1548
Bst4CI ACNGT 6 cut(s) 220, 862, 886, 1543, 1680, 1738
Bst6I CTCTTC 3 cut(s) 240, 315, 1080
BstAFI CTTAAG 1 cut(s) 560
BstAPI GCANNNNNTGC 1 cut(s) 1595
BstC8I GCNNGC 5 cut(s) 45, 636, 751, 1567, 1732
BstDEI CTNAG 5 cut(s) 251, 629, 1142, 1174, 1719
BstDSI CCRYGG 1 cut(s) 1737
BstENI CCTNNNNNAGG 1 cut(s) 1289
BstF5I GGATG 2 cut(s) 898, 1600
BstFNI CGCG 6 cut(s) 76, 387, 636, 905, 985, 1098
BstHHI GCGC 3 cut(s) 78, 636, 638
BstMAI GTCTC 1 cut(s) 587
BstMCI CGRYCG 1 cut(s) 909
BstMWI GCNNNNNNNGC 6 cut(s) 118, 393, 413, 1104, 1351, 1595
BstNI CCWGG 3 cut(s) 1168, 1534, 1548
BstNSI RCATGY 2 cut(s) 361, 1132
BstSCI CCNGG 4 cut(s) 526, 1166, 1532, 1546
BstSFI CTRYAG 3 cut(s) 1105, 1375, 1567
BstSLI GKGCMC 3 cut(s) 781, 1650, 1678
BstUI CGCG 6 cut(s) 76, 387, 636, 905, 985, 1098
BstV1I GCAGC 5 cut(s) 300, 360, 817, 1393, 1581
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 1 cut(s) 1031
BstXI CCANNNNNNTGG 1 cut(s) 1585
BstYI RGATCY 1 cut(s) 1031
Bsu36I CCTNAGG 1 cut(s) 1142
BsuI GTATCC 2 cut(s) 373, 1046
BsuRI GGCC 6 cut(s) 47, 155, 610, 764, 1392, 1532
BtgI CCRYGG 1 cut(s) 1737
BtsCI GGATG 2 cut(s) 898, 1600
BtsI GCAGTG 3 cut(s) 732, 776, 1121
BtsIMutI CAGTG 4 cut(s) 732, 776, 1121, 1419
BveI ACCTGC 1 cut(s) 1112
Cac8I GCNNGC 5 cut(s) 45, 636, 751, 1567, 1732
CfoI GCGC 3 cut(s) 78, 636, 638
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 7 cut(s) 55, 95, 608, 1258, 1282, 1553, 1605
CseI GACGC 1 cut(s) 911
Csp6I GTAC 6 cut(s) 115, 538, 571, 963, 1060, 1715
CviAII CATG 8 cut(s) 122, 242, 358, 979, 1129, 1403, 1415, 1590
CviQI GTAC 6 cut(s) 115, 538, 571, 963, 1060, 1715
DdeI CTNAG 5 cut(s) 251, 629, 1142, 1174, 1719
DraI TTTAAA 1 cut(s) 1243
EaeI YGGCCR 3 cut(s) 153, 762, 1530
Eam1104I CTCTTC 3 cut(s) 240, 315, 1080
EarI CTCTTC 3 cut(s) 240, 315, 1080
Eco130I CCWWGG 2 cut(s) 999, 1261
Eco24I GRGCYC 1 cut(s) 815
Eco31I GGTCTC 1 cut(s) 587
Eco32I GATATC 1 cut(s) 471
Eco47I GGWCC 6 cut(s) 55, 95, 1258, 1282, 1553, 1605
Eco57I CTGAAG 1 cut(s) 845
Eco81I CCTNAGG 1 cut(s) 1142
Eco88I CYCGRG 4 cut(s) 541, 806, 1182, 1235
EcoNI CCTNNNNNAGG 1 cut(s) 1289
EcoO109I RGGNCCY 1 cut(s) 1282
EcoRI GAATTC 2 cut(s) 9, 1692
EcoRII CCWGG 3 cut(s) 1166, 1532, 1546
EcoRV GATATC 1 cut(s) 471
EcoT14I CCWWGG 2 cut(s) 999, 1261
EcoT38I GRGCYC 1 cut(s) 815
ErhI CCWWGG 2 cut(s) 999, 1261
FaeI CATG 8 cut(s) 125, 245, 361, 982, 1132, 1406, 1418, 1593
FalI AAGNNNNNCTT 2 cut(s) 543, 575
FaqI GGGAC 1 cut(s) 1172
FatI CATG 8 cut(s) 121, 241, 357, 978, 1128, 1402, 1414, 1589
FauI CCCGC 1 cut(s) 392
FauNDI CATATG 2 cut(s) 613, 937
FbaI TGATCA 3 cut(s) 178, 654, 1248
Fnu4HI GCNGC 6 cut(s) 289, 374, 762, 831, 1407, 1570
FokI GGATG 2 cut(s) 905, 1607
FriOI GRGCYC 1 cut(s) 815
Fsp4HI GCNGC 6 cut(s) 289, 374, 762, 831, 1407, 1570
FspBI CTAG 1 cut(s) 1686
GlaI GCGC 3 cut(s) 77, 635, 637
GluI GCNGC 6 cut(s) 289, 374, 762, 831, 1407, 1570
GsuI CTGGAG 3 cut(s) 124, 466, 1569
HaeIII GGCC 6 cut(s) 47, 155, 610, 764, 1392, 1532
HapII CCGG 3 cut(s) 285, 528, 699
HgaI GACGC 1 cut(s) 911
HhaI GCGC 3 cut(s) 78, 636, 638
Hin1II CATG 8 cut(s) 125, 245, 361, 982, 1132, 1406, 1418, 1593
Hin6I GCGC 3 cut(s) 76, 634, 636
HinP1I GCGC 3 cut(s) 76, 634, 636
HincII GTYRAC 1 cut(s) 1683
HindII GTYRAC 1 cut(s) 1683
HindIII AAGCTT 1 cut(s) 1656
HinfI GANTC 7 cut(s) 38, 268, 423, 448, 710, 804, 1185
HpaII CCGG 3 cut(s) 285, 528, 699
HphI GGTGA 6 cut(s) 188, 232, 566, 807, 857, 1027
Hpy166II GTNNAC 7 cut(s) 95, 557, 779, 865, 1648, 1676, 1683
Hpy188III TCNNGA 9 cut(s) 65, 410, 452, 699, 707, 890, 1235, 1616, 1697
Hpy8I GTNNAC 7 cut(s) 95, 557, 779, 865, 1648, 1676, 1683
Hpy99I CGWCG 1 cut(s) 1367
HpyAV CCTTC 4 cut(s) 545, 802, 820, 1342
HpyCH4III ACNGT 6 cut(s) 220, 862, 886, 1543, 1680, 1738
HpyCH4V TGCA 9 cut(s) 779, 833, 845, 1107, 1126, 1220, 1569, 1648, 1676
HpyF10VI GCNNNNNNNGC 6 cut(s) 118, 393, 413, 1104, 1351, 1595
HpyF3I CTNAG 5 cut(s) 251, 629, 1142, 1174, 1719
Hsp92II CATG 8 cut(s) 125, 245, 361, 982, 1132, 1406, 1418, 1593
HspAI GCGC 3 cut(s) 76, 634, 636
Kpn2I TCCGGA 1 cut(s) 698
Ksp22I TGATCA 3 cut(s) 178, 654, 1248
LguI GCTCTTC 1 cut(s) 240
LmnI GCTCC 4 cut(s) 255, 789, 1309, 1351
Lsp1109I GCAGC 5 cut(s) 300, 360, 817, 1393, 1581
LweI GCATC 4 cut(s) 97, 403, 1458, 1585
MaeI CTAG 1 cut(s) 1686
MaeIII GTNAC 4 cut(s) 214, 740, 943, 1417
MboII GAAGA 9 cut(s) 209, 257, 332, 683, 923, 1097, 1278, 1444, 1507
MfeI CAATTG 1 cut(s) 207
MflI RGATCY 1 cut(s) 1031
MhlI GDGCHC 5 cut(s) 781, 815, 1650, 1678, 1736
MlsI TGGCCA 1 cut(s) 155
MluCI AATT 7 cut(s) 9, 207, 334, 897, 1041, 1269, 1692
MluNI TGGCCA 1 cut(s) 155
MlyI GAGTC 4 cut(s) 32, 457, 798, 1194
MmeI TCCRAC 3 cut(s) 400, 781, 1361
Mox20I TGGCCA 1 cut(s) 155
MroI TCCGGA 1 cut(s) 698
MroXI GAANNNNTTC 1 cut(s) 427
MscI TGGCCA 1 cut(s) 155
MseI TTAA 3 cut(s) 561, 1008, 1242
MslI CAYNNNNRTG 3 cut(s) 315, 1131, 1583
Msp20I TGGCCA 1 cut(s) 155
MspA1I CMGCKG 1 cut(s) 1583
MspCI CTTAAG 1 cut(s) 560
MspI CCGG 3 cut(s) 285, 528, 699
MspR9I CCNGG 4 cut(s) 528, 1168, 1534, 1548
MunI CAATTG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 1444
MvaI CCWGG 3 cut(s) 1168, 1534, 1548
MvnI CGCG 6 cut(s) 76, 387, 636, 905, 985, 1098
MwoI GCNNNNNNNGC 6 cut(s) 118, 393, 413, 1104, 1351, 1595
NciI CCSGG 1 cut(s) 528
NdeI CATATG 2 cut(s) 613, 937
NlaIII CATG 8 cut(s) 125, 245, 361, 982, 1132, 1406, 1418, 1593
NlaIV GGNNCC 6 cut(s) 56, 257, 859, 1074, 1283, 1545
NmeAIII GCCGAG 1 cut(s) 1508
NmuCI GTSAC 3 cut(s) 214, 740, 1417
NspI RCATGY 2 cut(s) 361, 1132
OliI CACNNNNGTG 1 cut(s) 1583
PaeR7I CTCGAG 2 cut(s) 541, 806
PalAI GGCGCGCC 1 cut(s) 634
PauI GCGCGC 1 cut(s) 634
PciI ACATGT 1 cut(s) 1128
PciSI GCTCTTC 1 cut(s) 240
PctI GAATGC 1 cut(s) 1444
PdmI GAANNNNTTC 1 cut(s) 427
PfeI GAWTC 3 cut(s) 268, 423, 710
PflMI CCANNNNNTGG 1 cut(s) 771
PkrI GCNGC 6 cut(s) 290, 375, 763, 832, 1408, 1571
Ple19I CGATCG 1 cut(s) 909
PleI GAGTC 4 cut(s) 32, 456, 798, 1193
PpsI GAGTC 4 cut(s) 32, 456, 798, 1193
PpuMI RGGWCCY 1 cut(s) 1282
PscI ACATGT 1 cut(s) 1128
Psp5II RGGWCCY 1 cut(s) 1282
Psp6I CCWGG 3 cut(s) 1166, 1532, 1546
PspGI CCWGG 3 cut(s) 1166, 1532, 1546
PspN4I GGNNCC 6 cut(s) 56, 257, 859, 1074, 1283, 1545
PspPI GGNCC 7 cut(s) 55, 95, 608, 1258, 1282, 1553, 1605
PspPPI RGGWCCY 1 cut(s) 1282
PspXI VCTCGAGB 2 cut(s) 541, 806
PstI CTGCAG 2 cut(s) 1109, 1571
PsuI RGATCY 1 cut(s) 1031
PteI GCGCGC 1 cut(s) 634
PvuI CGATCG 1 cut(s) 909
PvuII CAGCTG 1 cut(s) 1583
RsaI GTAC 6 cut(s) 116, 539, 572, 964, 1061, 1716
RsaNI GTAC 6 cut(s) 115, 538, 571, 963, 1060, 1715
RseI CAYNNNNRTG 3 cut(s) 315, 1131, 1583
SapI GCTCTTC 1 cut(s) 240
SaqAI TTAA 3 cut(s) 561, 1008, 1242
SatI GCNGC 6 cut(s) 289, 374, 762, 831, 1407, 1570
Sau96I GGNCC 7 cut(s) 55, 95, 608, 1258, 1282, 1553, 1605
SchI GAGTC 4 cut(s) 32, 457, 798, 1194
ScrFI CCNGG 4 cut(s) 528, 1168, 1534, 1548
SduI GDGCHC 5 cut(s) 781, 815, 1650, 1678, 1736
SfaNI GCATC 4 cut(s) 97, 403, 1458, 1585
SfcI CTRYAG 3 cut(s) 1105, 1375, 1567
Sfr274I CTCGAG 2 cut(s) 541, 806
SgsI GGCGCGCC 1 cut(s) 634
SinI GGWCC 6 cut(s) 55, 95, 1258, 1282, 1553, 1605
SlaI CTCGAG 2 cut(s) 541, 806
SmiMI CAYNNNNRTG 3 cut(s) 315, 1131, 1583
SmlI CTYRAG 4 cut(s) 65, 541, 560, 806
SmoI CTYRAG 4 cut(s) 65, 541, 560, 806
Sse9I AATT 7 cut(s) 9, 207, 334, 897, 1041, 1269, 1692
SsiI CCGC 6 cut(s) 385, 620, 761, 1096, 1689, 1753
SspMI CTAG 1 cut(s) 1686
StyD4I CCNGG 4 cut(s) 526, 1166, 1532, 1546
StyI CCWWGG 2 cut(s) 999, 1261
TaaI ACNGT 6 cut(s) 220, 862, 886, 1543, 1680, 1738
TaqI TCGA 4 cut(s) 542, 807, 909, 1707
TaqII GACCGA 1 cut(s) 1570
TasI AATT 7 cut(s) 9, 207, 334, 897, 1041, 1269, 1692
TatI WGTACW 2 cut(s) 537, 1059
TauI GCSGC 1 cut(s) 764
TfiI GAWTC 3 cut(s) 268, 423, 710
Tru1I TTAA 3 cut(s) 561, 1008, 1242
Tru9I TTAA 3 cut(s) 561, 1008, 1242
TscAI CASTG 4 cut(s) 739, 776, 1128, 1426
TseFI GTSAC 3 cut(s) 214, 740, 1417
TseI GCWGC 5 cut(s) 288, 373, 830, 1406, 1569
Tsp45I GTSAC 3 cut(s) 214, 740, 1417
TspDTI ATGAA 8 cut(s) 258, 333, 388, 702, 866, 1053, 1451, 1739
TspRI CASTG 4 cut(s) 739, 776, 1128, 1426
Van91I CCANNNNNTGG 1 cut(s) 771
Vha464I CTTAAG 1 cut(s) 560
VneI GTGCAC 3 cut(s) 777, 1646, 1674
VpaK11BI GGWCC 6 cut(s) 55, 95, 1258, 1282, 1553, 1605
XagI CCTNNNNNAGG 1 cut(s) 1289
XapI RAATTY 4 cut(s) 9, 334, 1041, 1692
XceI RCATGY 2 cut(s) 361, 1132
XhoI CTCGAG 2 cut(s) 541, 806
XmnI GAANNNNTTC 1 cut(s) 427
XspI CTAG 1 cut(s) 1686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.