RchiOBHm_Chr5g0070221

Asparagine synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010661
Physical Location & Seq
Reverse (-)
76021705 .. 76025878
4174 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34548

Sequence Viewer

Length: 1761 bp
ATGTGTGGAATACTTGCCGTTCTGGGTTGCTCCGATGACTCTCAGGCCAAAAGGGTCAGGGTTCTCGAGCTTTCTCGCAGGTTGAAGCACCGGGGTCCAGATTGGAGTGGCTTGTATCAGCACGGTGATTGTTTCTTGGCCCATCAGAGGTTGGCAATAATTGATCCTGCTTCTGGAGATCAACCTCTGTTTAATGAAGACAAATCAATTGTTGTCACGGTCAATGGAGAGATTTACAACCATGAAGAACTGAGGAAGCGTCTGCCGAATCACAAGTTCCGAACCGGCAGTGATTGTGATGTCATTGCCCATCTGTATGAAGAGTATGGGGAGAACTTCGTGGACATGCTTGATGGCATGTTCTCTTTTGTGCTGCTGGATACCCGGGACAACAGCTTCATTGTTGCTCGTGATGCGATTGGAATCACTTCCCTCTACATGGGCTGGAGTCTTGATGGCTCAATTTGGATTTCATCAGAACTGAAAGGATTGAATGATGATTGTGAACACTTCGAGTCCTTTCCACCCGGTCACCTGTACTCAAGCAAAACAGGTGGACTAAAGAGGTGGTACAACCCCCAATGGTTCACAGAGTCCATTCCATCCACGCCATATGATCCTCTTGTTCTGAGGCGTGCCTTCGAAAGCGCTGTGATCAAAAGGCTGATGACTGATGTGCCTTTTGGAGTTCTTCTATCTGGAGGCTTAGATTCATCTTTGGTTGCCTCTATCACTGCTCGTCACCTGGCAGGCACAAAGGCGGCCAAGCAATGGGGAGCACAACTCCATTCTTTCTGTGTTGGATTGGAGGGTTCCCCAGATTTGAAGGCTGGAAAAGAAGTTGCAGACTATTTAGGAACAGTTCACCATGAGTTTCATTTTACTGTTCAGGATGGAATTGATGCCATAGAGGAAGTTATATACCATGTTGAAACATACGATGTTACCACTATTCGAGCCAGCACCCCTATGTTTCTTATGTCACGTAAGATCAAGTCTTTGGGGGTCAAGATGGTGATTTCTGGTGAGGGCTCTGATGAGATTTTCGGTGGATATCTCTACTTCCACAAAGCACCCAACAAGGAAGAGTTTCACCGCGAAACTTGCAGAAAGATCAAGGCACTGCACATGTACGATTGCCTTAGGGCAAACAAATCAACATCTGCTTGGGGCTTAGAAGCTCGAGTCCCTTTCTTGGACAAAGAGTTTATTAATGTTGCAATGAGCATTGATCCAGAGTTCAAGCTGATCAAAAAGGATGAAGGCAGGATTGAGAAATGGGTCCTCAGGAAGGCATTTGATGATGAGGAGAAGCCTTATCTGCCAAAGCACATTCTTTACAGACAGAAAGAACAATTCAGTGACGGTGTTGGCTACAGTTGGATTGATGGCCTGAAAGCCCATGCTGCTCTACATGTCAATGATAAGATGATGCAAAATGCTGCACATATCTTCCCACACAACACTCCCACTACTAAGGAAGGCTACTACTATAGGATGATTTTCGAGAGGTTCTTCCCACAGAACTCGGCTAGGCAGACTGTCCCAGGAGGACCAAGTGTGGCCTGCAGCACAGCCACGGCTATTGAGTGGGATGCTGAGTGGTCTAAGAATCTTGACCCATCTGGCAGGGCTGCCCTTGGAGTCCACAACTCGGCTTATGAGAATCAAGCAGCTGTTCCGAGTGGACTTGGGCCTGAAATCATTGAAAATGTTCCTCACATGAAAACAAGTGCCCAAGGAGTTGCAATTGCGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00250 ko01100 ko01110 map00250 map01100 map01110
Pfam Domains
Protein Families
Asn_synthase GATase_7

Protein Analysis

586

Amino Acids

65.76

Weight (kDa)

6.06

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase_6 PF13522 33 - 160 1.7e-33 Glutamine amidotransferase domain
GATase_7 PF13537 48 - 165 7.3e-40 Glutamine amidotransferase domain
DUF3700 PF12481 112 - 202 7.7e-12 Aluminium induced protein
Asn_synthase PF00733 210 - 361 2e-49 Asparagine synthase
Asn_synthase PF00733 359 - 511 1.2e-33 Asparagine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 69
AccB7I CCANNNNNTGG 1 cut(s) 771
AccII CGCG 1 cut(s) 1098
AciI CCGC 2 cut(s) 761, 1096
AclWI GGATC 3 cut(s) 158, 611, 1226
AcoI YGGCCR 1 cut(s) 762
AfaI GTAC 3 cut(s) 539, 572, 1133
AfeI AGCGCT 1 cut(s) 649
AfiI CCNNNNNNNGG 7 cut(s) 147, 173, 439, 771, 1195, 1291, 1654
AflIII ACRYGT 2 cut(s) 1128, 1414
AgsI TTSAA 6 cut(s) 85, 493, 826, 932, 1243, 1709
AjnI CCWGG 2 cut(s) 744, 1546
AluBI AGCT 6 cut(s) 70, 396, 1181, 1246, 1676, 1758
AluI AGCT 6 cut(s) 70, 396, 1181, 1246, 1676, 1758
Alw21I GWGCWC 1 cut(s) 781
AlwI GGATC 3 cut(s) 158, 611, 1226
Ama87I CYCGRG 3 cut(s) 65, 384, 1182
Aor51HI AGCGCT 1 cut(s) 649
AoxI GGCC 6 cut(s) 45, 138, 762, 1390, 1563, 1694
ApeKI GCWGC 6 cut(s) 373, 1406, 1442, 1569, 1634, 1673
AseI ATTAAT 1 cut(s) 1212
Asp700I GAANNNNTTC 3 cut(s) 427, 1089, 1713
AspLEI GCGC 1 cut(s) 650
AspS9I GGNCC 5 cut(s) 95, 139, 1282, 1553, 1694
AsuC2I CCSGG 4 cut(s) 92, 385, 386, 528
AsuHPI GGTGA 7 cut(s) 137, 524, 734, 857, 1027, 1037, 1085
AsuII TTCGAA 1 cut(s) 642
AvaI CYCGRG 3 cut(s) 65, 384, 1182
AvaII GGWCC 3 cut(s) 95, 1282, 1553
AxyI CCTNAGG 2 cut(s) 1142, 1286
BaeGI GKGCMC 1 cut(s) 1738
BanII GRGCYC 1 cut(s) 1034
BauI CACGAG 1 cut(s) 408
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 781
BbvI GCAGC 6 cut(s) 360, 1393, 1429, 1581, 1621, 1685
BccI CCATC 9 cut(s) 150, 318, 347, 449, 610, 887, 1006, 1382, 1630
BceAI ACGGC 2 cut(s) 2, 1596
BciT130I CCWGG 2 cut(s) 746, 1548
BciVI GTATCC 1 cut(s) 373
BclI TGATCA 2 cut(s) 654, 1248
BcnI CCSGG 4 cut(s) 92, 385, 386, 528
BfaI CTAG 2 cut(s) 1533, 1759
BfmI CTRYAG 3 cut(s) 1375, 1492, 1567
BfoI RGCGCY 1 cut(s) 651
BfuAI ACCTGC 1 cut(s) 69
BfuI GTATCC 1 cut(s) 373
BisI GCNGC 7 cut(s) 374, 762, 1407, 1443, 1570, 1635, 1674
BlsI GCNGC 7 cut(s) 375, 763, 1408, 1444, 1571, 1636, 1675
Bme1390I CCNGG 6 cut(s) 92, 385, 386, 528, 746, 1548
Bme18I GGWCC 3 cut(s) 95, 1282, 1553
BmeT110I CYCGRG 3 cut(s) 65, 384, 1182
BmgT120I GGNCC 5 cut(s) 95, 139, 1282, 1553, 1694
BmiI GGNNCC 3 cut(s) 96, 814, 1283
BmrFI CCNGG 6 cut(s) 92, 385, 386, 528, 746, 1548
BmsI GCATC 4 cut(s) 403, 892, 1422, 1585
BpiI GAAGAC 1 cut(s) 204
BplI GAGNNNNNCTC 2 cut(s) 768, 800
BpmI CTGGAG 3 cut(s) 195, 466, 720
Bpu14I TTCGAA 1 cut(s) 642
BpuEI CTTGAG 1 cut(s) 526
BpuMI CCSGG 4 cut(s) 92, 385, 386, 528
BsaAI YACGTR 1 cut(s) 986
BsaBI GATNNNNATC 1 cut(s) 422
BsaJI CCNNGG 6 cut(s) 91, 384, 1546, 1578, 1639, 1738
Bsc4I CCNNNNNNNGG 7 cut(s) 147, 173, 439, 771, 1195, 1291, 1654
Bse118I RCCGGY 1 cut(s) 284
Bse21I CCTNAGG 2 cut(s) 1142, 1286
Bse3DI GCAATG 3 cut(s) 303, 776, 1227
Bse8I GATNNNNATC 1 cut(s) 422
BseBI CCWGG 2 cut(s) 746, 1548
BseDI CCNNGG 6 cut(s) 91, 384, 1546, 1578, 1639, 1738
BseGI GGATG 5 cut(s) 602, 898, 1264, 1503, 1600
BseJI GATNNNNATC 1 cut(s) 422
BseLI CCNNNNNNNGG 7 cut(s) 147, 173, 439, 771, 1195, 1291, 1654
BseMI GCAATG 3 cut(s) 303, 776, 1227
BseMII CTCAG 5 cut(s) 56, 242, 620, 1300, 1590
BseRI GAGGAG 1 cut(s) 1322
BseSI GKGCMC 1 cut(s) 1738
BseXI GCAGC 6 cut(s) 360, 1393, 1429, 1581, 1621, 1685
BsgI GTGCAG 2 cut(s) 1109, 1428
Bsh1236I CGCG 1 cut(s) 1098
BshFI GGCC 6 cut(s) 47, 140, 764, 1392, 1565, 1696
BsiHKAI GWGCWC 1 cut(s) 781
BsiHKCI CYCGRG 3 cut(s) 65, 384, 1182
BsiSI CCGG 4 cut(s) 91, 285, 385, 528
BslFI GGGAC 3 cut(s) 401, 1172, 1529
BslI CCNNNNNNNGG 7 cut(s) 147, 173, 439, 771, 1195, 1291, 1654
BsmFI GGGAC 3 cut(s) 401, 1172, 1529
BsnI GGCC 6 cut(s) 47, 140, 764, 1392, 1565, 1696
BsoBI CYCGRG 3 cut(s) 65, 384, 1182
Bsp119I TTCGAA 1 cut(s) 642
Bsp1286I GDGCHC 3 cut(s) 781, 1034, 1738
Bsp143I GATC 8 cut(s) 163, 178, 616, 654, 990, 1113, 1231, 1248
BspACI CCGC 2 cut(s) 761, 1096
BspANI GGCC 6 cut(s) 47, 140, 764, 1392, 1565, 1696
BspCNI CTCAG 5 cut(s) 55, 243, 621, 1299, 1591
BspFNI CGCG 1 cut(s) 1098
BspLI GGNNCC 3 cut(s) 96, 814, 1283
BspMAI CTGCAG 1 cut(s) 1571
BspMI ACCTGC 1 cut(s) 69
BspPI GGATC 3 cut(s) 158, 611, 1226
BspT104I TTCGAA 1 cut(s) 642
BsrDI GCAATG 3 cut(s) 303, 776, 1227
BsrFI RCCGGY 1 cut(s) 284
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 6 cut(s) 91, 384, 1546, 1578, 1639, 1738
BssMI GATC 8 cut(s) 163, 178, 616, 654, 990, 1113, 1231, 1248
BssSI CACGAG 1 cut(s) 408
BssT1I CCWWGG 2 cut(s) 1639, 1738
Bst2BI CACGAG 1 cut(s) 408
Bst2UI CCWGG 2 cut(s) 746, 1548
Bst4CI ACNGT 7 cut(s) 125, 220, 862, 886, 1367, 1379, 1543
Bst6I CTCTTC 2 cut(s) 315, 1080
BstBAI YACGTR 1 cut(s) 986
BstBI TTCGAA 1 cut(s) 642
BstC8I GCNNGC 5 cut(s) 636, 751, 961, 1567, 1756
BstDSI CCRYGG 1 cut(s) 1578
BstEII GGTNACC 1 cut(s) 530
BstENI CCTNNNNNAGG 1 cut(s) 1289
BstF5I GGATG 5 cut(s) 602, 898, 1264, 1503, 1600
BstFNI CGCG 1 cut(s) 1098
BstH2I RGCGCY 1 cut(s) 651
BstHHI GCGC 1 cut(s) 650
BstKTI GATC 8 cut(s) 166, 181, 619, 657, 993, 1116, 1234, 1251
BstMBI GATC 8 cut(s) 163, 178, 616, 654, 990, 1113, 1231, 1248
BstMWI GCNNNNNNNGC 4 cut(s) 413, 1104, 1321, 1406
BstNI CCWGG 2 cut(s) 746, 1548
BstNSI RCATGY 4 cut(s) 349, 361, 1132, 1418
BstPI GGTNACC 1 cut(s) 530
BstSCI CCNGG 6 cut(s) 90, 383, 384, 526, 744, 1546
BstSFI CTRYAG 3 cut(s) 1375, 1492, 1567
BstSLI GKGCMC 1 cut(s) 1738
BstUI CGCG 1 cut(s) 1098
BstV1I GCAGC 6 cut(s) 360, 1393, 1429, 1581, 1621, 1685
BstV2I GAAGAC 1 cut(s) 204
Bsu36I CCTNAGG 2 cut(s) 1142, 1286
BsuI GTATCC 1 cut(s) 373
BsuRI GGCC 6 cut(s) 47, 140, 764, 1392, 1565, 1696
BtgI CCRYGG 1 cut(s) 1578
BtsCI GGATG 5 cut(s) 602, 898, 1264, 1503, 1600
BtsI GCAGTG 3 cut(s) 295, 732, 1121
BtsIMutI CAGTG 4 cut(s) 295, 732, 1121, 1366
BveI ACCTGC 1 cut(s) 69
Cac8I GCNNGC 5 cut(s) 636, 751, 961, 1567, 1756
CfoI GCGC 1 cut(s) 650
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 5 cut(s) 95, 139, 1282, 1553, 1694
Cfr9I CCCGGG 1 cut(s) 384
CseI GACGC 1 cut(s) 248
Csp6I GTAC 3 cut(s) 538, 571, 1132
CspCI CAANNNNNGTGG 4 cut(s) 535, 570, 1567, 1602
CviQI GTAC 3 cut(s) 538, 571, 1132
DpnI GATC 8 cut(s) 165, 180, 618, 656, 992, 1115, 1233, 1250
DpnII GATC 8 cut(s) 163, 178, 616, 654, 990, 1113, 1231, 1248
EaeI YGGCCR 1 cut(s) 762
Eam1104I CTCTTC 2 cut(s) 315, 1080
EarI CTCTTC 2 cut(s) 315, 1080
Eco130I CCWWGG 2 cut(s) 1639, 1738
Eco24I GRGCYC 1 cut(s) 1034
Eco32I GATATC 1 cut(s) 1055
Eco47I GGWCC 3 cut(s) 95, 1282, 1553
Eco47III AGCGCT 1 cut(s) 649
Eco81I CCTNAGG 2 cut(s) 1142, 1286
Eco88I CYCGRG 3 cut(s) 65, 384, 1182
Eco91I GGTNACC 1 cut(s) 530
EcoNI CCTNNNNNAGG 1 cut(s) 1289
EcoO109I RGGNCCY 1 cut(s) 1282
EcoO65I GGTNACC 1 cut(s) 530
EcoRII CCWGG 2 cut(s) 744, 1546
EcoRV GATATC 1 cut(s) 1055
EcoT14I CCWWGG 2 cut(s) 1639, 1738
EcoT38I GRGCYC 1 cut(s) 1034
ErhI CCWWGG 2 cut(s) 1639, 1738
FaqI GGGAC 3 cut(s) 401, 1172, 1529
FauNDI CATATG 1 cut(s) 613
FbaI TGATCA 2 cut(s) 654, 1248
Fnu4HI GCNGC 7 cut(s) 374, 762, 1407, 1443, 1570, 1635, 1674
FokI GGATG 5 cut(s) 589, 905, 1271, 1510, 1607
FriOI GRGCYC 1 cut(s) 1034
Fsp4HI GCNGC 7 cut(s) 374, 762, 1407, 1443, 1570, 1635, 1674
FspBI CTAG 2 cut(s) 1533, 1759
GlaI GCGC 1 cut(s) 649
GluI GCNGC 7 cut(s) 374, 762, 1407, 1443, 1570, 1635, 1674
GsuI CTGGAG 3 cut(s) 195, 466, 720
HaeII RGCGCY 1 cut(s) 651
HaeIII GGCC 6 cut(s) 47, 140, 764, 1392, 1565, 1696
HapII CCGG 4 cut(s) 91, 285, 385, 528
HgaI GACGC 1 cut(s) 248
HhaI GCGC 1 cut(s) 650
Hin6I GCGC 1 cut(s) 648
HinP1I GCGC 1 cut(s) 648
HpaII CCGG 4 cut(s) 91, 285, 385, 528
HphI GGTGA 7 cut(s) 137, 524, 734, 857, 1027, 1037, 1085
Hpy166II GTNNAC 7 cut(s) 343, 506, 557, 588, 865, 1648, 1688
Hpy188I TCNGA 7 cut(s) 34, 147, 281, 478, 630, 1036, 1683
Hpy8I GTNNAC 7 cut(s) 343, 506, 557, 588, 865, 1648, 1688
HpyAV CCTTC 5 cut(s) 649, 820, 1256, 1285, 1475
HpyCH4III ACNGT 7 cut(s) 125, 220, 862, 886, 1367, 1379, 1543
HpyCH4IV ACGT 1 cut(s) 985
HpyCH4V TGCA 8 cut(s) 845, 1107, 1126, 1220, 1435, 1445, 1569, 1748
HpyF10VI GCNNNNNNNGC 4 cut(s) 413, 1104, 1321, 1406
HpySE526I ACGT 1 cut(s) 985
HspAI GCGC 1 cut(s) 648
Ksp22I TGATCA 2 cut(s) 654, 1248
Kzo9I GATC 8 cut(s) 163, 178, 616, 654, 990, 1113, 1231, 1248
LmnI GCTCC 2 cut(s) 35, 776
Lsp1109I GCAGC 6 cut(s) 360, 1393, 1429, 1581, 1621, 1685
LweI GCATC 4 cut(s) 403, 892, 1422, 1585
MaeI CTAG 2 cut(s) 1533, 1759
MaeII ACGT 1 cut(s) 985
MaeIII GTNAC 6 cut(s) 214, 530, 740, 943, 981, 1361
MalI GATC 8 cut(s) 165, 180, 618, 656, 992, 1115, 1233, 1250
MboI GATC 8 cut(s) 163, 178, 616, 654, 990, 1113, 1231, 1248
MboII GAAGA 7 cut(s) 209, 257, 332, 683, 1097, 1444, 1507
MfeI CAATTG 2 cut(s) 207, 1749
MhlI GDGCHC 3 cut(s) 781, 1034, 1738
MluCI AATT 6 cut(s) 159, 207, 462, 897, 1355, 1749
MlyI GAGTC 6 cut(s) 32, 457, 524, 602, 1194, 1653
MmeI TCCRAC 2 cut(s) 781, 1361
MroXI GAANNNNTTC 3 cut(s) 427, 1089, 1713
MseI TTAA 2 cut(s) 192, 1212
MslI CAYNNNNRTG 3 cut(s) 315, 968, 1419
MspA1I CMGCKG 1 cut(s) 1676
MspI CCGG 4 cut(s) 91, 285, 385, 528
MspR9I CCNGG 6 cut(s) 92, 385, 386, 528, 746, 1548
MunI CAATTG 2 cut(s) 207, 1749
MvaI CCWGG 2 cut(s) 746, 1548
MvnI CGCG 1 cut(s) 1098
MwoI GCNNNNNNNGC 4 cut(s) 413, 1104, 1321, 1406
NciI CCSGG 4 cut(s) 92, 385, 386, 528
NdeI CATATG 1 cut(s) 613
NdeII GATC 8 cut(s) 163, 178, 616, 654, 990, 1113, 1231, 1248
NlaIV GGNNCC 3 cut(s) 96, 814, 1283
NmeAIII GCCGAG 2 cut(s) 1508, 1634
NmuCI GTSAC 5 cut(s) 214, 530, 740, 981, 1361
NspI RCATGY 4 cut(s) 349, 361, 1132, 1418
NspV TTCGAA 1 cut(s) 642
PaeR7I CTCGAG 2 cut(s) 65, 1182
PciI ACATGT 2 cut(s) 1128, 1414
PdmI GAANNNNTTC 3 cut(s) 427, 1089, 1713
PfeI GAWTC 5 cut(s) 268, 423, 710, 1612, 1666
PflMI CCANNNNNTGG 1 cut(s) 771
PkrI GCNGC 7 cut(s) 375, 763, 1408, 1444, 1571, 1636, 1675
PleI GAGTC 6 cut(s) 32, 456, 523, 601, 1193, 1652
PpsI GAGTC 6 cut(s) 32, 456, 523, 601, 1193, 1652
Ppu21I YACGTR 1 cut(s) 986
PpuMI RGGWCCY 1 cut(s) 1282
PscI ACATGT 2 cut(s) 1128, 1414
PshBI ATTAAT 1 cut(s) 1212
Psp5II RGGWCCY 1 cut(s) 1282
Psp6I CCWGG 2 cut(s) 744, 1546
PspEI GGTNACC 1 cut(s) 530
PspGI CCWGG 2 cut(s) 744, 1546
PspN4I GGNNCC 3 cut(s) 96, 814, 1283
PspPI GGNCC 5 cut(s) 95, 139, 1282, 1553, 1694
PspPPI RGGWCCY 1 cut(s) 1282
PspXI VCTCGAGB 1 cut(s) 1182
PstI CTGCAG 1 cut(s) 1571
PvuII CAGCTG 1 cut(s) 1676
RsaI GTAC 3 cut(s) 539, 572, 1133
RsaNI GTAC 3 cut(s) 538, 571, 1132
RseI CAYNNNNRTG 3 cut(s) 315, 968, 1419
SaqAI TTAA 2 cut(s) 192, 1212
SatI GCNGC 7 cut(s) 374, 762, 1407, 1443, 1570, 1635, 1674
Sau3AI GATC 8 cut(s) 163, 178, 616, 654, 990, 1113, 1231, 1248
Sau96I GGNCC 5 cut(s) 95, 139, 1282, 1553, 1694
SchI GAGTC 6 cut(s) 32, 457, 524, 602, 1194, 1653
ScrFI CCNGG 6 cut(s) 92, 385, 386, 528, 746, 1548
SduI GDGCHC 3 cut(s) 781, 1034, 1738
SfaNI GCATC 4 cut(s) 403, 892, 1422, 1585
SfcI CTRYAG 3 cut(s) 1375, 1492, 1567
Sfr274I CTCGAG 2 cut(s) 65, 1182
SfuI TTCGAA 1 cut(s) 642
SinI GGWCC 3 cut(s) 95, 1282, 1553
SlaI CTCGAG 2 cut(s) 65, 1182
SmaI CCCGGG 1 cut(s) 386
SmiMI CAYNNNNRTG 3 cut(s) 315, 968, 1419
SmlI CTYRAG 3 cut(s) 65, 541, 1182
SmoI CTYRAG 3 cut(s) 65, 541, 1182
Sse9I AATT 6 cut(s) 159, 207, 462, 897, 1355, 1749
SsiI CCGC 2 cut(s) 761, 1096
SspMI CTAG 2 cut(s) 1533, 1759
StyD4I CCNGG 6 cut(s) 90, 383, 384, 526, 744, 1546
StyI CCWWGG 2 cut(s) 1639, 1738
TaaI ACNGT 7 cut(s) 125, 220, 862, 886, 1367, 1379, 1543
TaiI ACGT 1 cut(s) 988
TaqI TCGA 6 cut(s) 66, 513, 642, 955, 1183, 1506
TasI AATT 6 cut(s) 159, 207, 462, 897, 1355, 1749
TatI WGTACW 1 cut(s) 537
TauI GCSGC 1 cut(s) 764
TfiI GAWTC 5 cut(s) 268, 423, 710, 1612, 1666
Tru1I TTAA 2 cut(s) 192, 1212
Tru9I TTAA 2 cut(s) 192, 1212
TscAI CASTG 4 cut(s) 295, 739, 1128, 1366
TseFI GTSAC 5 cut(s) 214, 530, 740, 981, 1361
TseI GCWGC 6 cut(s) 373, 1406, 1442, 1569, 1634, 1673
Tsp45I GTSAC 5 cut(s) 214, 530, 740, 981, 1361
TspDTI ATGAA 9 cut(s) 210, 258, 333, 388, 462, 702, 866, 1275, 1739
TspMI CCCGGG 1 cut(s) 384
TspRI CASTG 4 cut(s) 295, 739, 1128, 1366
Van91I CCANNNNNTGG 1 cut(s) 771
VpaK11BI GGWCC 3 cut(s) 95, 1282, 1553
VspI ATTAAT 1 cut(s) 1212
XagI CCTNNNNNAGG 1 cut(s) 1289
XceI RCATGY 4 cut(s) 349, 361, 1132, 1418
XhoI CTCGAG 2 cut(s) 65, 1182
XmaI CCCGGG 1 cut(s) 384
XmnI GAANNNNTTC 3 cut(s) 427, 1089, 1713
XspI CTAG 2 cut(s) 1533, 1759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.