Rw5G042890

Asparagine synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010661
Physical Location & Seq
Reverse (-)
74975622 .. 74979771
4150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G042890.1

Sequence Viewer

Length: 1350 bp
ATGTGTGGAATACTTGCCGTTCTGGGTTGCTCCGATGACTCTCAGGCCAAAAGGGTCAGGGTTCTCGAGCTTTCTCGCAGGTTGAAGCACCGGGGTCCAGATTGGAGTGGTCTGTATCAGCACGGTGATTGTTTCTTGGCCCATCAGAGGTTGGCAATTATTGATCCTGCTTCTGGAGATCAACCTCTGTTTAATGAAGACAAATCTATTGTTGTCACGGTCAATGGAGAGATTTACAACCATGAAGAACTGAGGAAGCGTCTGCCGAATCACAAGTTCCGAACCGGCAGTGATTGTGATGTCATTGCCCATCTGTATGAAGAGTATGGGGAGAACTTTGTGGACATGCTTGATGGCATGTTCTCTTTTGTGCTGCTGGATACCCGGGACAACAGCTTCATTGTTGCTCGTGATGCTATTGGAATCACTTCCCTCTACATGGGCTGGAGTCTTGATGGCTCAATTTGGATTTCATCAGAACTGAAAGGATTGAATGATGATTGTGAACACTTCGAGTCCTTTCCACCTGGTCACCTGTACTCAAGCAAAACAGGTGGACTAAAGAGGTGGTACAACCCCCAATGGTTCACAGAGTCCATTCCATCTACGCCATATGATCCTCTTGTTCTGAGGCGTGCCTTTGAAAGCGCTGTGATCAAAAGGCTGATGACTGATGTGCCTTTTGGAGTTCTTCTATCTGGAGGCTTAGATTCATCTTTGGTTGCCTCTATCACTGCTCGTCACCTGGCAGGCACAAAGGCGGCCAAGCAATGGGGAGCACAACTCCATTCTTTCTGCGTTGGATTGGAGGGTTCCCCAGATTTGAAGGCTGGAAAAGAAGTTGCAGACTATTTGGGAACAGTTCACCATGAGTTTCATTTTACTGTACAGGATGGAATTGATGCCATAGAGGAAGTTATATACCATGTTGAAACATACGATGTTACCACTATTCGAGCCAGCACCCCTATGTTTCTTATGTCACGTAAGATCAAGTCTTTGGGGGTCAAGATGGTGATTTCTGGTGAGGGCTCTGATGAGATTTTTGGTGGATATCTCTACTTCCACAAAGCACCCAACAAGGAAGAGTTTCACCGTGAAACTTGCAGAAAGATCAAGGCACTGCACATGTACGATTGCCTTAGGGCAAACAAATCAACATCTGCTTGGGGCTTAGAAGCTCGAGTCCCTTTCTTGGACAAAGAGTTTATTAATGTTGCAATGAGCATTGATCCAGAGTTCAAGCTGATCAAAAAGGATGAAGGCAGGATTGAGAAATGGGTCCTCAGGAAGGCATTTGATGATGAGGAGAAGCCTTATCTGCCAAAGGTATACCTTCTTCTATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00250 ko01100 ko01110 map00250 map01100 map01110
Pfam Domains
Protein Families
Asn_synthase GATase_7

Protein Analysis

449

Amino Acids

50.86

Weight (kDa)

5.95

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase_6 PF13522 33 - 160 1e-33 Glutamine amidotransferase domain
GATase_7 PF13537 48 - 165 4.6e-40 Glutamine amidotransferase domain
DUF3700 PF12481 112 - 202 5.1e-12 Aluminium induced protein
Asn_synthase PF00733 210 - 361 1.4e-49 Asparagine synthase
Asn_synthase PF00733 359 - 443 1.7e-21 Asparagine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 69
AccB7I CCANNNNNTGG 1 cut(s) 771
AccI GTMKAC 1 cut(s) 1332
AciI CCGC 1 cut(s) 761
AclWI GGATC 3 cut(s) 158, 611, 1226
AcoI YGGCCR 1 cut(s) 762
AfaI GTAC 4 cut(s) 539, 572, 888, 1133
AfeI AGCGCT 1 cut(s) 649
AfiI CCNNNNNNNGG 6 cut(s) 147, 173, 439, 771, 1195, 1291
AflIII ACRYGT 1 cut(s) 1128
AgsI TTSAA 6 cut(s) 85, 493, 644, 826, 932, 1243
AjnI CCWGG 2 cut(s) 526, 744
AluBI AGCT 4 cut(s) 70, 396, 1181, 1246
AluI AGCT 4 cut(s) 70, 396, 1181, 1246
Alw21I GWGCWC 1 cut(s) 781
AlwI GGATC 3 cut(s) 158, 611, 1226
Ama87I CYCGRG 3 cut(s) 65, 384, 1182
Aor51HI AGCGCT 1 cut(s) 649
AoxI GGCC 3 cut(s) 45, 138, 762
ApeKI GCWGC 1 cut(s) 373
AseI ATTAAT 1 cut(s) 1212
Asp700I GAANNNNTTC 2 cut(s) 427, 1089
AspLEI GCGC 1 cut(s) 650
AspS9I GGNCC 3 cut(s) 95, 139, 1282
AsuC2I CCSGG 3 cut(s) 92, 385, 386
AsuHPI GGTGA 7 cut(s) 137, 524, 734, 857, 1027, 1037, 1085
AvaI CYCGRG 3 cut(s) 65, 384, 1182
AvaII GGWCC 2 cut(s) 95, 1282
AxyI CCTNAGG 2 cut(s) 1142, 1286
BanII GRGCYC 1 cut(s) 1034
BarI GAAGNNNNNNTAC 1 cut(s) 1323
BauI CACGAG 1 cut(s) 408
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 781
BbvI GCAGC 1 cut(s) 360
BccI CCATC 7 cut(s) 150, 318, 347, 449, 610, 887, 1006
BceAI ACGGC 1 cut(s) 2
BciT130I CCWGG 2 cut(s) 528, 746
BciVI GTATCC 1 cut(s) 373
BclI TGATCA 2 cut(s) 654, 1248
BcnI CCSGG 3 cut(s) 92, 385, 386
BfoI RGCGCY 1 cut(s) 651
BfuAI ACCTGC 1 cut(s) 69
BfuI GTATCC 1 cut(s) 373
BisI GCNGC 2 cut(s) 374, 762
BlsI GCNGC 2 cut(s) 375, 763
Bme1390I CCNGG 5 cut(s) 92, 385, 386, 528, 746
Bme18I GGWCC 2 cut(s) 95, 1282
BmeT110I CYCGRG 3 cut(s) 65, 384, 1182
BmgT120I GGNCC 3 cut(s) 95, 139, 1282
BmiI GGNNCC 3 cut(s) 96, 814, 1283
BmrFI CCNGG 5 cut(s) 92, 385, 386, 528, 746
BmsI GCATC 2 cut(s) 403, 892
BpiI GAAGAC 1 cut(s) 204
BplI GAGNNNNNCTC 2 cut(s) 768, 800
BpmI CTGGAG 3 cut(s) 195, 466, 720
BpuEI CTTGAG 1 cut(s) 526
BpuMI CCSGG 3 cut(s) 92, 385, 386
BsaAI YACGTR 1 cut(s) 986
BsaJI CCNNGG 2 cut(s) 91, 384
Bsc4I CCNNNNNNNGG 6 cut(s) 147, 173, 439, 771, 1195, 1291
Bse118I RCCGGY 1 cut(s) 284
Bse21I CCTNAGG 2 cut(s) 1142, 1286
Bse3DI GCAATG 3 cut(s) 303, 776, 1227
BseBI CCWGG 2 cut(s) 528, 746
BseDI CCNNGG 2 cut(s) 91, 384
BseGI GGATG 2 cut(s) 898, 1264
BseLI CCNNNNNNNGG 6 cut(s) 147, 173, 439, 771, 1195, 1291
BseMI GCAATG 3 cut(s) 303, 776, 1227
BseMII CTCAG 4 cut(s) 56, 242, 620, 1300
BseRI GAGGAG 1 cut(s) 1322
BseXI GCAGC 1 cut(s) 360
BsgI GTGCAG 1 cut(s) 1109
BshFI GGCC 3 cut(s) 47, 140, 764
BsiHKAI GWGCWC 1 cut(s) 781
BsiHKCI CYCGRG 3 cut(s) 65, 384, 1182
BsiSI CCGG 3 cut(s) 91, 285, 385
BslFI GGGAC 2 cut(s) 401, 1172
BslI CCNNNNNNNGG 6 cut(s) 147, 173, 439, 771, 1195, 1291
BsmFI GGGAC 2 cut(s) 401, 1172
BsnI GGCC 3 cut(s) 47, 140, 764
BsoBI CYCGRG 3 cut(s) 65, 384, 1182
Bsp1286I GDGCHC 2 cut(s) 781, 1034
Bsp1407I TGTACA 1 cut(s) 886
Bsp143I GATC 8 cut(s) 163, 178, 616, 654, 990, 1113, 1231, 1248
BspACI CCGC 1 cut(s) 761
BspANI GGCC 3 cut(s) 47, 140, 764
BspCNI CTCAG 4 cut(s) 55, 243, 621, 1299
BspLI GGNNCC 3 cut(s) 96, 814, 1283
BspMI ACCTGC 1 cut(s) 69
BspPI GGATC 3 cut(s) 158, 611, 1226
BsrDI GCAATG 3 cut(s) 303, 776, 1227
BsrFI RCCGGY 1 cut(s) 284
BsrGI TGTACA 1 cut(s) 886
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 2 cut(s) 91, 384
BssMI GATC 8 cut(s) 163, 178, 616, 654, 990, 1113, 1231, 1248
BssNAI GTATAC 1 cut(s) 1333
BssSI CACGAG 1 cut(s) 408
Bst1107I GTATAC 1 cut(s) 1333
Bst2BI CACGAG 1 cut(s) 408
Bst2UI CCWGG 2 cut(s) 528, 746
Bst4CI ACNGT 5 cut(s) 125, 220, 862, 886, 1097
Bst6I CTCTTC 2 cut(s) 315, 1080
BstAUI TGTACA 1 cut(s) 886
BstBAI YACGTR 1 cut(s) 986
BstC8I GCNNGC 3 cut(s) 636, 751, 961
BstDEI CTNAG 7 cut(s) 42, 251, 629, 706, 1142, 1174, 1286
BstEII GGTNACC 1 cut(s) 530
BstENI CCTNNNNNAGG 1 cut(s) 1289
BstF5I GGATG 2 cut(s) 898, 1264
BstH2I RGCGCY 1 cut(s) 651
BstHHI GCGC 1 cut(s) 650
BstKTI GATC 8 cut(s) 166, 181, 619, 657, 993, 1116, 1234, 1251
BstMBI GATC 8 cut(s) 163, 178, 616, 654, 990, 1113, 1231, 1248
BstMWI GCNNNNNNNGC 2 cut(s) 413, 1321
BstNI CCWGG 2 cut(s) 528, 746
BstNSI RCATGY 3 cut(s) 349, 361, 1132
BstPI GGTNACC 1 cut(s) 530
BstSCI CCNGG 5 cut(s) 90, 383, 384, 526, 744
BstV1I GCAGC 1 cut(s) 360
BstV2I GAAGAC 1 cut(s) 204
BstZ17I GTATAC 1 cut(s) 1333
Bsu36I CCTNAGG 2 cut(s) 1142, 1286
BsuI GTATCC 1 cut(s) 373
BsuRI GGCC 3 cut(s) 47, 140, 764
BtsCI GGATG 2 cut(s) 898, 1264
BtsI GCAGTG 3 cut(s) 295, 732, 1121
BtsIMutI CAGTG 3 cut(s) 295, 732, 1121
BveI ACCTGC 1 cut(s) 69
Cac8I GCNNGC 3 cut(s) 636, 751, 961
CfoI GCGC 1 cut(s) 650
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 3 cut(s) 95, 139, 1282
Cfr9I CCCGGG 1 cut(s) 384
CseI GACGC 1 cut(s) 248
CsiI ACCWGGT 1 cut(s) 526
Csp6I GTAC 4 cut(s) 538, 571, 887, 1132
CspCI CAANNNNNGTGG 2 cut(s) 535, 570
CviAII CATG 7 cut(s) 242, 346, 358, 439, 869, 926, 1129
CviQI GTAC 4 cut(s) 538, 571, 887, 1132
DdeI CTNAG 7 cut(s) 42, 251, 629, 706, 1142, 1174, 1286
DpnI GATC 8 cut(s) 165, 180, 618, 656, 992, 1115, 1233, 1250
DpnII GATC 8 cut(s) 163, 178, 616, 654, 990, 1113, 1231, 1248
EaeI YGGCCR 1 cut(s) 762
Eam1104I CTCTTC 2 cut(s) 315, 1080
EarI CTCTTC 2 cut(s) 315, 1080
Eco24I GRGCYC 1 cut(s) 1034
Eco32I GATATC 1 cut(s) 1055
Eco47I GGWCC 2 cut(s) 95, 1282
Eco47III AGCGCT 1 cut(s) 649
Eco81I CCTNAGG 2 cut(s) 1142, 1286
Eco88I CYCGRG 3 cut(s) 65, 384, 1182
Eco91I GGTNACC 1 cut(s) 530
EcoNI CCTNNNNNAGG 1 cut(s) 1289
EcoO109I RGGNCCY 1 cut(s) 1282
EcoO65I GGTNACC 1 cut(s) 530
EcoRII CCWGG 2 cut(s) 526, 744
EcoRV GATATC 1 cut(s) 1055
EcoT38I GRGCYC 1 cut(s) 1034
FaeI CATG 7 cut(s) 245, 349, 361, 442, 872, 929, 1132
FaqI GGGAC 2 cut(s) 401, 1172
FatI CATG 7 cut(s) 241, 345, 357, 438, 868, 925, 1128
FauNDI CATATG 1 cut(s) 613
FbaI TGATCA 2 cut(s) 654, 1248
FblI GTMKAC 1 cut(s) 1332
Fnu4HI GCNGC 2 cut(s) 374, 762
FokI GGATG 2 cut(s) 905, 1271
FriOI GRGCYC 1 cut(s) 1034
Fsp4HI GCNGC 2 cut(s) 374, 762
GlaI GCGC 1 cut(s) 649
GluI GCNGC 2 cut(s) 374, 762
GsuI CTGGAG 3 cut(s) 195, 466, 720
HaeII RGCGCY 1 cut(s) 651
HaeIII GGCC 3 cut(s) 47, 140, 764
HapII CCGG 3 cut(s) 91, 285, 385
HgaI GACGC 1 cut(s) 248
HhaI GCGC 1 cut(s) 650
Hin1II CATG 7 cut(s) 245, 349, 361, 442, 872, 929, 1132
Hin6I GCGC 1 cut(s) 648
HinP1I GCGC 1 cut(s) 648
HinfI GANTC 8 cut(s) 38, 268, 423, 448, 515, 593, 710, 1185
HpaII CCGG 3 cut(s) 91, 285, 385
HphI GGTGA 7 cut(s) 137, 524, 734, 857, 1027, 1037, 1085
Hpy166II GTNNAC 6 cut(s) 343, 506, 557, 588, 865, 1333
Hpy188I TCNGA 6 cut(s) 34, 147, 281, 478, 630, 1036
Hpy188III TCNNGA 9 cut(s) 65, 98, 174, 410, 452, 699, 1009, 1235, 1288
Hpy8I GTNNAC 6 cut(s) 343, 506, 557, 588, 865, 1333
HpyAV CCTTC 4 cut(s) 820, 1256, 1285, 1346
HpyCH4III ACNGT 5 cut(s) 125, 220, 862, 886, 1097
HpyCH4IV ACGT 1 cut(s) 985
HpyCH4V TGCA 4 cut(s) 845, 1107, 1126, 1220
HpyF10VI GCNNNNNNNGC 2 cut(s) 413, 1321
HpyF3I CTNAG 7 cut(s) 42, 251, 629, 706, 1142, 1174, 1286
HpySE526I ACGT 1 cut(s) 985
Hsp92II CATG 7 cut(s) 245, 349, 361, 442, 872, 929, 1132
HspAI GCGC 1 cut(s) 648
Ksp22I TGATCA 2 cut(s) 654, 1248
Kzo9I GATC 8 cut(s) 163, 178, 616, 654, 990, 1113, 1231, 1248
LmnI GCTCC 2 cut(s) 35, 776
Lsp1109I GCAGC 1 cut(s) 360
LweI GCATC 2 cut(s) 403, 892
MabI ACCWGGT 1 cut(s) 526
MaeII ACGT 1 cut(s) 985
MaeIII GTNAC 5 cut(s) 214, 530, 740, 943, 981
MalI GATC 8 cut(s) 165, 180, 618, 656, 992, 1115, 1233, 1250
MboI GATC 8 cut(s) 163, 178, 616, 654, 990, 1113, 1231, 1248
MboII GAAGA 6 cut(s) 209, 257, 332, 683, 1097, 1331
MhlI GDGCHC 2 cut(s) 781, 1034
MluCI AATT 3 cut(s) 156, 462, 897
MlyI GAGTC 5 cut(s) 32, 457, 524, 602, 1194
MmeI TCCRAC 1 cut(s) 781
MroXI GAANNNNTTC 2 cut(s) 427, 1089
MseI TTAA 2 cut(s) 192, 1212
MslI CAYNNNNRTG 2 cut(s) 315, 968
MspI CCGG 3 cut(s) 91, 285, 385
MspR9I CCNGG 5 cut(s) 92, 385, 386, 528, 746
MvaI CCWGG 2 cut(s) 528, 746
MwoI GCNNNNNNNGC 2 cut(s) 413, 1321
NciI CCSGG 3 cut(s) 92, 385, 386
NdeI CATATG 1 cut(s) 613
NdeII GATC 8 cut(s) 163, 178, 616, 654, 990, 1113, 1231, 1248
NlaIII CATG 7 cut(s) 245, 349, 361, 442, 872, 929, 1132
NlaIV GGNNCC 3 cut(s) 96, 814, 1283
NmuCI GTSAC 4 cut(s) 214, 530, 740, 981
NspI RCATGY 3 cut(s) 349, 361, 1132
PaeR7I CTCGAG 2 cut(s) 65, 1182
PciI ACATGT 1 cut(s) 1128
PdmI GAANNNNTTC 2 cut(s) 427, 1089
PfeI GAWTC 3 cut(s) 268, 423, 710
PflMI CCANNNNNTGG 1 cut(s) 771
PkrI GCNGC 2 cut(s) 375, 763
PleI GAGTC 5 cut(s) 32, 456, 523, 601, 1193
PpsI GAGTC 5 cut(s) 32, 456, 523, 601, 1193
Ppu21I YACGTR 1 cut(s) 986
PpuMI RGGWCCY 1 cut(s) 1282
PscI ACATGT 1 cut(s) 1128
PshBI ATTAAT 1 cut(s) 1212
Psp5II RGGWCCY 1 cut(s) 1282
Psp6I CCWGG 2 cut(s) 526, 744
PspEI GGTNACC 1 cut(s) 530
PspGI CCWGG 2 cut(s) 526, 744
PspN4I GGNNCC 3 cut(s) 96, 814, 1283
PspPI GGNCC 3 cut(s) 95, 139, 1282
PspPPI RGGWCCY 1 cut(s) 1282
PspXI VCTCGAGB 1 cut(s) 1182
RsaI GTAC 4 cut(s) 539, 572, 888, 1133
RsaNI GTAC 4 cut(s) 538, 571, 887, 1132
RseI CAYNNNNRTG 2 cut(s) 315, 968
SaqAI TTAA 2 cut(s) 192, 1212
SatI GCNGC 2 cut(s) 374, 762
Sau3AI GATC 8 cut(s) 163, 178, 616, 654, 990, 1113, 1231, 1248
Sau96I GGNCC 3 cut(s) 95, 139, 1282
SchI GAGTC 5 cut(s) 32, 457, 524, 602, 1194
ScrFI CCNGG 5 cut(s) 92, 385, 386, 528, 746
SduI GDGCHC 2 cut(s) 781, 1034
SexAI ACCWGGT 1 cut(s) 526
SfaNI GCATC 2 cut(s) 403, 892
Sfr274I CTCGAG 2 cut(s) 65, 1182
SinI GGWCC 2 cut(s) 95, 1282
SlaI CTCGAG 2 cut(s) 65, 1182
SmaI CCCGGG 1 cut(s) 386
SmiMI CAYNNNNRTG 2 cut(s) 315, 968
SmlI CTYRAG 3 cut(s) 65, 541, 1182
SmoI CTYRAG 3 cut(s) 65, 541, 1182
Sse9I AATT 3 cut(s) 156, 462, 897
SsiI CCGC 1 cut(s) 761
StyD4I CCNGG 5 cut(s) 90, 383, 384, 526, 744
TaaI ACNGT 5 cut(s) 125, 220, 862, 886, 1097
TaiI ACGT 1 cut(s) 988
TaqI TCGA 4 cut(s) 66, 513, 955, 1183
TasI AATT 3 cut(s) 156, 462, 897
TatI WGTACW 2 cut(s) 537, 886
TauI GCSGC 1 cut(s) 764
TfiI GAWTC 3 cut(s) 268, 423, 710
Tru1I TTAA 2 cut(s) 192, 1212
Tru9I TTAA 2 cut(s) 192, 1212
TscAI CASTG 3 cut(s) 295, 739, 1128
TseFI GTSAC 4 cut(s) 214, 530, 740, 981
TseI GCWGC 1 cut(s) 373
Tsp45I GTSAC 4 cut(s) 214, 530, 740, 981
TspDTI ATGAA 8 cut(s) 210, 258, 333, 388, 462, 702, 866, 1275
TspMI CCCGGG 1 cut(s) 384
TspRI CASTG 3 cut(s) 295, 739, 1128
Van91I CCANNNNNTGG 1 cut(s) 771
VpaK11BI GGWCC 2 cut(s) 95, 1282
VspI ATTAAT 1 cut(s) 1212
XagI CCTNNNNNAGG 1 cut(s) 1289
XceI RCATGY 3 cut(s) 349, 361, 1132
XhoI CTCGAG 2 cut(s) 65, 1182
XmaI CCCGGG 1 cut(s) 384
XmiI GTMKAC 1 cut(s) 1332
XmnI GAANNNNTTC 2 cut(s) 427, 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.