MD11G1070800.v1.1

Asparagine synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010661
Physical Location & Seq
Forward (+)
6056815 .. 6061464
4650 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1070800.v1.1.491

Sequence Viewer

Length: 1761 bp
ATGTGTGGAATTCTTGCCGTTCTTGGTTGCTCCGATGACTCTCAGGCCAAGAGGGTCCGAGTTCTTGAGCTTTCTCGCAGATTGAAGCACCGCGGACCTGATTGGAGCGGTCTATACCAGCATGGAGATTGTTTCTTGGCTCATCAGAGGCTGGCCATTATTGACCCTGCTTCTGGTGATCAACCTCTCTACAATGAGGACAAATCAATTGTTGTCACGGTGAATGGAGAGATTTACAACCATGAAGAACTGAGGAGCCGTCTGCCGAATCATAAGTTCCGAACCGGCAGTGACTGTGATGTCATTGCCCATCTGTATGAAGAGTATGGGGAGAATTTCGTGGATATGCTGGACGGCATGTTTTCGTTTGTGCTGCTGGATACCCGCGACAACAGCTTCATCGTTGCTCGTGATGCTATCGGAATCACTTCCCTCTATATCGGCTGGAGTCTTGACGGCTCGGTTTGGATATCATCGGAACTGAAAGGGTTGAATGATGACTGCGAACACTTTGAGAGCTTTCCGCCCGGTCATCTGTACTCAAGCAAAGAAGGTGCACTGAAGAGGTGGTACAACCCTACTTGGTTCTCGGAGACCATTCCATCAGGGCCATATGATCCGCTTGTTCTGAGGCGCGCTTTTGAAGCTGCTGTGATCAAAAGGCTAATGACCGATGTGCCTTTCGGTGTTCTTCTCTCTGGGGGTCTGGATTCATCTTTGGTCGCCTCTATCACTGCTCGTCACTTGGCAGGCACGAAGGCGGCCAAGCAATGGGGAGCGCAACTCCATTCCTTCTGTGTTGGACTCGAGGGCTCGCCCGATCTGAAGGCTGGGAAAGAAGTTGCAGACTATTTGGGGACCGTTCACCACGAGTTCCACTTTACTGTTCAGGATGGGATCGATGCTATCGAGGAAGTTATCTACCACATTGAAACATATGATGTTACCACTATCCGAGCAAGTACCCCTATGTTTCTCATGTCACGTAAGATCAAGTCGTTGGGGGTCAAGATGGTGATTTCTGGTGAGGGATCCGATGAGATTTTCGGTGGATACTTGTACTTCCACAAGGCACCCAACAAGGAAGAGTTTCACCGCGAAACTTGCAGAAAGATTAAGGCACTGCACATGTATGATTGCCTCAGGGCAAACAAATCAACATCTGCTTGGGGATTAGAAGCTCGAGTCCCATTCCTGGACAAAGATTTCATTAATGTTGCAATGGATATTGATCCCGAGTTTAAAATGATCAAAAAGGACCAAGGAAGAATTGAGAAATGGGTCCTCCGGAAGGCGTTTGATGATGAGGAGAAACCTTATCTGCCAAAGCACATTCTGTATAGGCAGAAAGAGCAGTTCAGTGACGGGGTCGGCTACAGTTGGATTGATGGCCTGAAAGCTCATGCTGAACTCCATGTCACTGATAAGATGATGCTGAATGCTGAACGCATCTTCCCTCACAACACTCCTTATACAAAGGAAGCCTACTACTATCGAATGATTTTCGAGAGGTTCTTCCCACAGAACTCGGCCAGGAACACGGTGCCTGGAGGACCGAGTGTGGCCTGCAGCACAGCCACAGCTGTGGCATGGGATGCTGAATGGTCCAAAAATCTTGACCCGTCTGGTAGGGCTATACTTGAAGTGCACCAACAAGCTTATGACAAGCAGGGTGCACCGGTGACTAGCGGCATTCCAGAGATCATCGACAATGTACCTCAGATGAAAGCGAGCACCGTGGGGCTCGCAATCCACAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00250 ko01100 ko01110 map00250 map01100 map01110
Pfam Domains
Protein Families
Asn_synthase GATase_7

Protein Analysis

587

Amino Acids

66.03

Weight (kDa)

5.92

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase_6 PF13522 33 - 160 3e-33 Glutamine amidotransferase domain
GATase_7 PF13537 48 - 165 2.2e-39 Glutamine amidotransferase domain
DUF3700 PF12481 112 - 195 7.6e-11 Aluminium induced protein
Asn_synthase PF00733 210 - 361 1.2e-49 Asparagine synthase
Asn_synthase PF00733 358 - 511 2.7e-32 Asparagine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 299
AccB1I GGYRCC 2 cut(s) 1072, 1543
AccB7I CCANNNNNTGG 1 cut(s) 771
AccBSI CCGCTC 1 cut(s) 108
AccII CGCG 4 cut(s) 93, 387, 636, 1098
AccIII TCCGGA 1 cut(s) 1287
AciI CCGC 9 cut(s) 91, 93, 108, 385, 524, 620, 761, 1096, 1689
AclWI GGATC 5 cut(s) 611, 905, 1026, 1039, 1226
AcoI YGGCCR 3 cut(s) 153, 762, 1530
AcsI RAATTY 2 cut(s) 9, 334
AcuI CTGAAG 2 cut(s) 581, 845
AfaI GTAC 5 cut(s) 539, 572, 964, 1061, 1716
AfiI CCNNNNNNNGG 4 cut(s) 173, 771, 1195, 1291
AflIII ACRYGT 1 cut(s) 1128
AgeI ACCGGT 1 cut(s) 1678
AgsI TTSAA 5 cut(s) 85, 493, 644, 932, 1643
AjnI CCWGG 3 cut(s) 1194, 1532, 1546
AleI CACNNNNGTG 1 cut(s) 1583
AloI GAACNNNNNNTCC 2 cut(s) 1437, 1469
AluBI AGCT 9 cut(s) 70, 396, 519, 647, 1181, 1400, 1583, 1658, 1758
AluI AGCT 9 cut(s) 70, 396, 519, 647, 1181, 1400, 1583, 1658, 1758
Alw21I GWGCWC 4 cut(s) 559, 1650, 1678, 1736
Alw26I GTCTC 1 cut(s) 587
Alw44I GTGCAC 3 cut(s) 555, 1646, 1674
AlwI GGATC 5 cut(s) 611, 905, 1026, 1039, 1226
AlwNI CAGNNNCTG 2 cut(s) 151, 294
Ama87I CYCGRG 3 cut(s) 806, 1182, 1235
Aor13HI TCCGGA 1 cut(s) 1287
AoxI GGCC 7 cut(s) 45, 153, 608, 762, 1390, 1530, 1563
ApaLI GTGCAC 3 cut(s) 555, 1646, 1674
ApeKI GCWGC 3 cut(s) 373, 647, 1569
ApoI RAATTY 2 cut(s) 9, 334
AseI ATTAAT 1 cut(s) 1212
AsiGI ACCGGT 1 cut(s) 1678
Asp700I GAANNNNTTC 2 cut(s) 427, 1089
AspLEI GCGC 3 cut(s) 636, 638, 781
AspS9I GGNCC 8 cut(s) 55, 95, 608, 858, 1258, 1282, 1553, 1605
AsuC2I CCSGG 1 cut(s) 528
AsuHPI GGTGA 7 cut(s) 188, 232, 857, 1027, 1037, 1085, 1693
AvaI CYCGRG 3 cut(s) 806, 1182, 1235
AvaII GGWCC 7 cut(s) 55, 95, 858, 1258, 1282, 1553, 1605
AxyI CCTNAGG 1 cut(s) 1142
BaeGI GKGCMC 3 cut(s) 559, 1650, 1678
BalI TGGCCA 1 cut(s) 155
BamHI GGATCC 1 cut(s) 1031
BanI GGYRCC 2 cut(s) 1072, 1543
BanII GRGCYC 2 cut(s) 815, 1746
BarI GAAGNNNNNNTAC 2 cut(s) 554, 586
BauI CACGAG 2 cut(s) 408, 869
Bbv12I GWGCWC 4 cut(s) 559, 1650, 1678, 1736
BbvI GCAGC 3 cut(s) 360, 634, 1581
BccI CCATC 5 cut(s) 318, 610, 887, 1006, 1382
BceAI ACGGC 4 cut(s) 2, 243, 370, 472
BciT130I CCWGG 3 cut(s) 1196, 1534, 1548
BciVI GTATCC 2 cut(s) 373, 1046
BclI TGATCA 3 cut(s) 178, 654, 1248
BcnI CCSGG 1 cut(s) 528
BcoDI GTCTC 1 cut(s) 587
BfaI CTAG 2 cut(s) 1686, 1759
BfmI CTRYAG 2 cut(s) 1375, 1567
BfuI GTATCC 2 cut(s) 373, 1046
BisI GCNGC 5 cut(s) 374, 648, 762, 1570, 1690
BlsI GCNGC 5 cut(s) 375, 649, 763, 1571, 1691
Bme1390I CCNGG 4 cut(s) 528, 1196, 1534, 1548
Bme18I GGWCC 7 cut(s) 55, 95, 858, 1258, 1282, 1553, 1605
BmeT110I CYCGRG 3 cut(s) 806, 1182, 1235
BmgT120I GGNCC 8 cut(s) 55, 95, 608, 858, 1258, 1282, 1553, 1605
BmiI GGNNCC 7 cut(s) 56, 257, 859, 1033, 1074, 1283, 1545
BmrFI CCNGG 4 cut(s) 528, 1196, 1534, 1548
BmsI GCATC 5 cut(s) 403, 892, 1422, 1458, 1585
BplI GAGNNNNNCTC 2 cut(s) 768, 800
BpmI CTGGAG 2 cut(s) 466, 1569
BpuEI CTTGAG 2 cut(s) 86, 526
BpuMI CCSGG 1 cut(s) 528
Bsa29I ATCGAT 1 cut(s) 900
BsaAI YACGTR 1 cut(s) 986
BsaBI GATNNNNATC 1 cut(s) 1230
BsaI GGTCTC 1 cut(s) 587
BsaJI CCNNGG 3 cut(s) 91, 1261, 1737
BsaWI WCCGGW 2 cut(s) 1287, 1678
Bsc4I CCNNNNNNNGG 4 cut(s) 173, 771, 1195, 1291
Bse118I RCCGGY 2 cut(s) 284, 1678
Bse21I CCTNAGG 1 cut(s) 1142
Bse3DI GCAATG 3 cut(s) 303, 776, 1227
Bse8I GATNNNNATC 1 cut(s) 1230
BseAI TCCGGA 1 cut(s) 1287
BseBI CCWGG 3 cut(s) 1196, 1534, 1548
BseCI ATCGAT 1 cut(s) 900
BseDI CCNNGG 3 cut(s) 91, 1261, 1737
BseGI GGATG 2 cut(s) 898, 1600
BseJI GATNNNNATC 1 cut(s) 1230
BseLI CCNNNNNNNGG 4 cut(s) 173, 771, 1195, 1291
BseMI GCAATG 3 cut(s) 303, 776, 1227
BseMII CTCAG 5 cut(s) 56, 242, 620, 1156, 1733
BsePI GCGCGC 1 cut(s) 634
BseRI GAGGAG 2 cut(s) 268, 1322
BseSI GKGCMC 3 cut(s) 559, 1650, 1678
BseXI GCAGC 3 cut(s) 360, 634, 1581
BseYI CCCAGC 1 cut(s) 830
BsgI GTGCAG 1 cut(s) 1109
Bsh1236I CGCG 4 cut(s) 93, 387, 636, 1098
BshFI GGCC 7 cut(s) 47, 155, 610, 764, 1392, 1532, 1565
BshNI GGYRCC 2 cut(s) 1072, 1543
BshTI ACCGGT 1 cut(s) 1678
BshVI ATCGAT 1 cut(s) 900
BsiHKAI GWGCWC 4 cut(s) 559, 1650, 1678, 1736
BsiHKCI CYCGRG 3 cut(s) 806, 1182, 1235
BsiSI CCGG 4 cut(s) 285, 528, 1288, 1679
BslFI GGGAC 2 cut(s) 871, 1172
BslI CCNNNNNNNGG 4 cut(s) 173, 771, 1195, 1291
BsmAI GTCTC 1 cut(s) 587
BsmFI GGGAC 2 cut(s) 871, 1172
BsmI GAATGC 2 cut(s) 1444, 1692
BsnI GGCC 7 cut(s) 47, 155, 610, 764, 1392, 1532, 1565
Bso31I GGTCTC 1 cut(s) 587
BsoBI CYCGRG 3 cut(s) 806, 1182, 1235
Bsp1286I GDGCHC 6 cut(s) 559, 815, 1650, 1678, 1736, 1746
Bsp13I TCCGGA 1 cut(s) 1287
BspACI CCGC 9 cut(s) 91, 93, 108, 385, 524, 620, 761, 1096, 1689
BspANI GGCC 7 cut(s) 47, 155, 610, 764, 1392, 1532, 1565
BspCNI CTCAG 5 cut(s) 55, 243, 621, 1155, 1732
BspDI ATCGAT 1 cut(s) 900
BspEI TCCGGA 1 cut(s) 1287
BspFNI CGCG 4 cut(s) 93, 387, 636, 1098
BspLI GGNNCC 7 cut(s) 56, 257, 859, 1033, 1074, 1283, 1545
BspMAI CTGCAG 1 cut(s) 1571
BspPI GGATC 5 cut(s) 611, 905, 1026, 1039, 1226
BspT107I GGYRCC 2 cut(s) 1072, 1543
BspTNI GGTCTC 1 cut(s) 587
BsrBI CCGCTC 1 cut(s) 108
BsrDI GCAATG 3 cut(s) 303, 776, 1227
BsrFI RCCGGY 2 cut(s) 284, 1678
BssAI RCCGGY 2 cut(s) 284, 1678
BssECI CCNNGG 3 cut(s) 91, 1261, 1737
BssHII GCGCGC 1 cut(s) 634
BssSI CACGAG 2 cut(s) 408, 869
BssT1I CCWWGG 1 cut(s) 1261
Bst2BI CACGAG 2 cut(s) 408, 869
Bst2UI CCWGG 3 cut(s) 1196, 1534, 1548
Bst4CI ACNGT 7 cut(s) 220, 296, 862, 886, 1379, 1543, 1738
Bst6I CTCTTC 3 cut(s) 315, 557, 1080
BstAPI GCANNNNNTGC 1 cut(s) 1595
BstBAI YACGTR 1 cut(s) 986
BstC8I GCNNGC 7 cut(s) 153, 636, 751, 815, 1567, 1732, 1746
BstDEI CTNAG 5 cut(s) 42, 251, 629, 1142, 1719
BstDSI CCRYGG 2 cut(s) 91, 1737
BstENI CCTNNNNNAGG 1 cut(s) 1289
BstF5I GGATG 2 cut(s) 898, 1600
BstFNI CGCG 4 cut(s) 93, 387, 636, 1098
BstHHI GCGC 3 cut(s) 636, 638, 781
BstMAI GTCTC 1 cut(s) 587
BstMWI GCNNNNNNNGC 6 cut(s) 393, 413, 644, 1104, 1351, 1595
BstNI CCWGG 3 cut(s) 1196, 1534, 1548
BstNSI RCATGY 2 cut(s) 361, 1132
BstSCI CCNGG 4 cut(s) 526, 1194, 1532, 1546
BstSFI CTRYAG 2 cut(s) 1375, 1567
BstSLI GKGCMC 3 cut(s) 559, 1650, 1678
BstUI CGCG 4 cut(s) 93, 387, 636, 1098
BstV1I GCAGC 3 cut(s) 360, 634, 1581
BstX2I RGATCY 1 cut(s) 1031
BstXI CCANNNNNNTGG 1 cut(s) 1585
BstYI RGATCY 1 cut(s) 1031
Bsu15I ATCGAT 1 cut(s) 900
Bsu36I CCTNAGG 1 cut(s) 1142
BsuI GTATCC 2 cut(s) 373, 1046
BsuRI GGCC 7 cut(s) 47, 155, 610, 764, 1392, 1532, 1565
BsuTUI ATCGAT 1 cut(s) 900
BtgI CCRYGG 2 cut(s) 91, 1737
BtsCI GGATG 2 cut(s) 898, 1600
BtsI GCAGTG 3 cut(s) 295, 732, 1121
BtsIMutI CAGTG 6 cut(s) 295, 557, 732, 1121, 1366, 1419
Cac8I GCNNGC 7 cut(s) 153, 636, 751, 815, 1567, 1732, 1746
CaiI CAGNNNCTG 2 cut(s) 151, 294
CfoI GCGC 3 cut(s) 636, 638, 781
Cfr10I RCCGGY 2 cut(s) 284, 1678
Cfr13I GGNCC 8 cut(s) 55, 95, 608, 858, 1258, 1282, 1553, 1605
Cfr42I CCGCGG 1 cut(s) 94
ClaI ATCGAT 1 cut(s) 900
Csp6I GTAC 5 cut(s) 538, 571, 963, 1060, 1715
CspAI ACCGGT 1 cut(s) 1678
CviAII CATG 8 cut(s) 122, 242, 358, 979, 1129, 1403, 1415, 1590
CviQI GTAC 5 cut(s) 538, 571, 963, 1060, 1715
DdeI CTNAG 5 cut(s) 42, 251, 629, 1142, 1719
DraI TTTAAA 1 cut(s) 1243
DrdI GACNNNNNNGTC 1 cut(s) 299
DseDI GACNNNNNNGTC 1 cut(s) 299
EaeI YGGCCR 3 cut(s) 153, 762, 1530
Eam1104I CTCTTC 3 cut(s) 315, 557, 1080
EarI CTCTTC 3 cut(s) 315, 557, 1080
EciI GGCGGA 1 cut(s) 513
Eco130I CCWWGG 1 cut(s) 1261
Eco24I GRGCYC 2 cut(s) 815, 1746
Eco31I GGTCTC 1 cut(s) 587
Eco32I GATATC 1 cut(s) 471
Eco47I GGWCC 7 cut(s) 55, 95, 858, 1258, 1282, 1553, 1605
Eco57I CTGAAG 2 cut(s) 581, 845
Eco81I CCTNAGG 1 cut(s) 1142
Eco88I CYCGRG 3 cut(s) 806, 1182, 1235
EcoNI CCTNNNNNAGG 1 cut(s) 1289
EcoO109I RGGNCCY 1 cut(s) 1282
EcoRI GAATTC 1 cut(s) 9
EcoRII CCWGG 3 cut(s) 1194, 1532, 1546
EcoRV GATATC 1 cut(s) 471
EcoT14I CCWWGG 1 cut(s) 1261
EcoT38I GRGCYC 2 cut(s) 815, 1746
ErhI CCWWGG 1 cut(s) 1261
FaeI CATG 8 cut(s) 125, 245, 361, 982, 1132, 1406, 1418, 1593
FaqI GGGAC 2 cut(s) 871, 1172
FatI CATG 8 cut(s) 121, 241, 357, 978, 1128, 1402, 1414, 1589
FauI CCCGC 1 cut(s) 392
FauNDI CATATG 2 cut(s) 613, 937
FbaI TGATCA 3 cut(s) 178, 654, 1248
Fnu4HI GCNGC 5 cut(s) 374, 648, 762, 1570, 1690
FokI GGATG 2 cut(s) 905, 1607
FriOI GRGCYC 2 cut(s) 815, 1746
Fsp4HI GCNGC 5 cut(s) 374, 648, 762, 1570, 1690
FspBI CTAG 2 cut(s) 1686, 1759
GlaI GCGC 3 cut(s) 635, 637, 780
GluI GCNGC 5 cut(s) 374, 648, 762, 1570, 1690
GsaI CCCAGC 1 cut(s) 834
GsuI CTGGAG 2 cut(s) 466, 1569
HaeIII GGCC 7 cut(s) 47, 155, 610, 764, 1392, 1532, 1565
HapII CCGG 4 cut(s) 285, 528, 1288, 1679
HhaI GCGC 3 cut(s) 636, 638, 781
Hin1II CATG 8 cut(s) 125, 245, 361, 982, 1132, 1406, 1418, 1593
Hin6I GCGC 3 cut(s) 634, 636, 779
HinP1I GCGC 3 cut(s) 634, 636, 779
HindIII AAGCTT 1 cut(s) 1656
HinfI GANTC 7 cut(s) 38, 268, 423, 448, 710, 804, 1185
HpaII CCGG 4 cut(s) 285, 528, 1288, 1679
HphI GGTGA 7 cut(s) 188, 232, 857, 1027, 1037, 1085, 1693
Hpy166II GTNNAC 4 cut(s) 557, 865, 1648, 1676
Hpy8I GTNNAC 4 cut(s) 557, 865, 1648, 1676
HpyAV CCTTC 5 cut(s) 545, 751, 802, 820, 1285
HpyCH4III ACNGT 7 cut(s) 220, 296, 862, 886, 1379, 1543, 1738
HpyCH4IV ACGT 1 cut(s) 985
HpyCH4V TGCA 8 cut(s) 557, 845, 1107, 1126, 1220, 1569, 1648, 1676
HpyF10VI GCNNNNNNNGC 6 cut(s) 393, 413, 644, 1104, 1351, 1595
HpyF3I CTNAG 5 cut(s) 42, 251, 629, 1142, 1719
HpySE526I ACGT 1 cut(s) 985
Hsp92II CATG 8 cut(s) 125, 245, 361, 982, 1132, 1406, 1418, 1593
HspAI GCGC 3 cut(s) 634, 636, 779
Kpn2I TCCGGA 1 cut(s) 1287
Ksp22I TGATCA 3 cut(s) 178, 654, 1248
KspI CCGCGG 1 cut(s) 94
LmnI GCTCC 4 cut(s) 35, 105, 255, 776
Lsp1109I GCAGC 3 cut(s) 360, 634, 1581
LweI GCATC 5 cut(s) 403, 892, 1422, 1458, 1585
MaeI CTAG 2 cut(s) 1686, 1759
MaeII ACGT 1 cut(s) 985
MaeIII GTNAC 8 cut(s) 214, 290, 740, 943, 981, 1361, 1417, 1681
MbiI CCGCTC 1 cut(s) 108
MboII GAAGA 8 cut(s) 257, 332, 574, 683, 1097, 1278, 1444, 1507
MfeI CAATTG 1 cut(s) 207
MflI RGATCY 1 cut(s) 1031
MhlI GDGCHC 6 cut(s) 559, 815, 1650, 1678, 1736, 1746
MlsI TGGCCA 1 cut(s) 155
MluCI AATT 4 cut(s) 9, 207, 334, 1269
MluNI TGGCCA 1 cut(s) 155
MlyI GAGTC 4 cut(s) 32, 457, 798, 1194
MmeI TCCRAC 2 cut(s) 781, 1361
Mox20I TGGCCA 1 cut(s) 155
MroI TCCGGA 1 cut(s) 1287
MroXI GAANNNNTTC 2 cut(s) 427, 1089
MscI TGGCCA 1 cut(s) 155
MseI TTAA 3 cut(s) 1116, 1212, 1242
MslI CAYNNNNRTG 3 cut(s) 315, 1131, 1583
Msp20I TGGCCA 1 cut(s) 155
MspA1I CMGCKG 2 cut(s) 93, 1583
MspI CCGG 4 cut(s) 285, 528, 1288, 1679
MspR9I CCNGG 4 cut(s) 528, 1196, 1534, 1548
MunI CAATTG 1 cut(s) 207
Mva1269I GAATGC 2 cut(s) 1444, 1692
MvaI CCWGG 3 cut(s) 1196, 1534, 1548
MvnI CGCG 4 cut(s) 93, 387, 636, 1098
MwoI GCNNNNNNNGC 6 cut(s) 393, 413, 644, 1104, 1351, 1595
NciI CCSGG 1 cut(s) 528
NdeI CATATG 2 cut(s) 613, 937
NlaIII CATG 8 cut(s) 125, 245, 361, 982, 1132, 1406, 1418, 1593
NlaIV GGNNCC 7 cut(s) 56, 257, 859, 1033, 1074, 1283, 1545
NmeAIII GCCGAG 1 cut(s) 1508
NmuCI GTSAC 7 cut(s) 214, 290, 740, 981, 1361, 1417, 1681
NspI RCATGY 2 cut(s) 361, 1132
OliI CACNNNNGTG 1 cut(s) 1583
PaeR7I CTCGAG 2 cut(s) 806, 1182
PauI GCGCGC 1 cut(s) 634
PciI ACATGT 1 cut(s) 1128
PcsI WCGNNNNNNNCGW 1 cut(s) 906
PctI GAATGC 2 cut(s) 1444, 1692
PdmI GAANNNNTTC 2 cut(s) 427, 1089
PfeI GAWTC 3 cut(s) 268, 423, 710
PflFI GACNNNGTC 1 cut(s) 1367
PflMI CCANNNNNTGG 1 cut(s) 771
PfoI TCCNGGA 1 cut(s) 1194
PinAI ACCGGT 1 cut(s) 1678
PkrI GCNGC 5 cut(s) 375, 649, 763, 1571, 1691
PleI GAGTC 4 cut(s) 32, 456, 798, 1193
PpsI GAGTC 4 cut(s) 32, 456, 798, 1193
Ppu21I YACGTR 1 cut(s) 986
PpuMI RGGWCCY 1 cut(s) 1282
PscI ACATGT 1 cut(s) 1128
PshBI ATTAAT 1 cut(s) 1212
Psp5II RGGWCCY 1 cut(s) 1282
Psp6I CCWGG 3 cut(s) 1194, 1532, 1546
PspFI CCCAGC 1 cut(s) 830
PspGI CCWGG 3 cut(s) 1194, 1532, 1546
PspN4I GGNNCC 7 cut(s) 56, 257, 859, 1033, 1074, 1283, 1545
PspPI GGNCC 8 cut(s) 55, 95, 608, 858, 1258, 1282, 1553, 1605
PspPPI RGGWCCY 1 cut(s) 1282
PspXI VCTCGAGB 2 cut(s) 806, 1182
PstI CTGCAG 1 cut(s) 1571
PstNI CAGNNNCTG 2 cut(s) 151, 294
PsuI RGATCY 1 cut(s) 1031
PsyI GACNNNGTC 1 cut(s) 1367
PteI GCGCGC 1 cut(s) 634
PvuII CAGCTG 1 cut(s) 1583
RsaI GTAC 5 cut(s) 539, 572, 964, 1061, 1716
RsaNI GTAC 5 cut(s) 538, 571, 963, 1060, 1715
RseI CAYNNNNRTG 3 cut(s) 315, 1131, 1583
SacII CCGCGG 1 cut(s) 94
SaqAI TTAA 3 cut(s) 1116, 1212, 1242
SatI GCNGC 5 cut(s) 374, 648, 762, 1570, 1690
Sau96I GGNCC 8 cut(s) 55, 95, 608, 858, 1258, 1282, 1553, 1605
SchI GAGTC 4 cut(s) 32, 457, 798, 1194
ScrFI CCNGG 4 cut(s) 528, 1196, 1534, 1548
SduI GDGCHC 6 cut(s) 559, 815, 1650, 1678, 1736, 1746
SfaNI GCATC 5 cut(s) 403, 892, 1422, 1458, 1585
SfcI CTRYAG 2 cut(s) 1375, 1567
Sfr274I CTCGAG 2 cut(s) 806, 1182
Sfr303I CCGCGG 1 cut(s) 94
SgrAI CRCCGGYG 1 cut(s) 1678
SgrBI CCGCGG 1 cut(s) 94
SinI GGWCC 7 cut(s) 55, 95, 858, 1258, 1282, 1553, 1605
SlaI CTCGAG 2 cut(s) 806, 1182
SmiMI CAYNNNNRTG 3 cut(s) 315, 1131, 1583
SmlI CTYRAG 4 cut(s) 65, 541, 806, 1182
SmoI CTYRAG 4 cut(s) 65, 541, 806, 1182
Sse9I AATT 4 cut(s) 9, 207, 334, 1269
SsiI CCGC 9 cut(s) 91, 93, 108, 385, 524, 620, 761, 1096, 1689
SspMI CTAG 2 cut(s) 1686, 1759
StyD4I CCNGG 4 cut(s) 526, 1194, 1532, 1546
StyI CCWWGG 1 cut(s) 1261
TaaI ACNGT 7 cut(s) 220, 296, 862, 886, 1379, 1543, 1738
TaiI ACGT 1 cut(s) 988
TaqI TCGA 7 cut(s) 807, 900, 909, 1183, 1495, 1506, 1707
TaqII GACCGA 2 cut(s) 686, 1570
TasI AATT 4 cut(s) 9, 207, 334, 1269
TatI WGTACW 2 cut(s) 537, 1059
TauI GCSGC 2 cut(s) 764, 1692
TfiI GAWTC 3 cut(s) 268, 423, 710
Tru1I TTAA 3 cut(s) 1116, 1212, 1242
Tru9I TTAA 3 cut(s) 1116, 1212, 1242
TscAI CASTG 6 cut(s) 295, 564, 739, 1128, 1366, 1426
TseFI GTSAC 7 cut(s) 214, 290, 740, 981, 1361, 1417, 1681
TseI GCWGC 3 cut(s) 373, 647, 1569
Tsp45I GTSAC 7 cut(s) 214, 290, 740, 981, 1361, 1417, 1681
TspDTI ATGAA 6 cut(s) 258, 333, 388, 702, 1198, 1739
TspRI CASTG 6 cut(s) 295, 564, 739, 1128, 1366, 1426
Tth111I GACNNNGTC 1 cut(s) 1367
Van91I CCANNNNNTGG 1 cut(s) 771
VneI GTGCAC 3 cut(s) 555, 1646, 1674
VpaK11BI GGWCC 7 cut(s) 55, 95, 858, 1258, 1282, 1553, 1605
VspI ATTAAT 1 cut(s) 1212
XagI CCTNNNNNAGG 1 cut(s) 1289
XapI RAATTY 2 cut(s) 9, 334
XceI RCATGY 2 cut(s) 361, 1132
XhoI CTCGAG 2 cut(s) 806, 1182
XmnI GAANNNNTTC 2 cut(s) 427, 1089
XspI CTAG 2 cut(s) 1686, 1759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.