Rmu_sc0024476.1_g000001

Asparagine synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0024476.1
OG0010661
Physical Location & Seq
Reverse (-)
2 .. 519
518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0024476.1_g000001.1.cds

Sequence Viewer

Length: 305 bp
ctgtactcaagcaaaacaggtggactaaagaggtggtacaacccccaatggtttacagagtccattccatctacgccatatgatcctcttgttctgaggcgtgcctttgaaagcgctgtgatcaaaaggctgatgactgatgtgccttttggagttcttctatctggaggcttagattcatctttggttgcctctatcactgctcgtcacctggcaggcacaaaggcggccaagcaatggggagcacaactccattctttctgtgttggattggagggttccccagatttgaaggctggaaaaga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00250 ko01100 ko01110 map00250 map01100 map01110
Pfam Domains
Protein Families
Asn_synthase GATase_7

Protein Analysis

101

Amino Acids

10.95

Weight (kDa)

9.67

Isoelectric Point (pI)

51.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 237
AciI CCGC 1 cut(s) 227
AclWI GGATC 1 cut(s) 77
AcoI YGGCCR 1 cut(s) 228
AfaI GTAC 2 cut(s) 5, 38
AfeI AGCGCT 1 cut(s) 115
AfiI CCNNNNNNNGG 1 cut(s) 237
AgsI TTSAA 2 cut(s) 110, 292
AjnI CCWGG 1 cut(s) 210
Alw21I GWGCWC 1 cut(s) 247
AlwI GGATC 1 cut(s) 77
Aor51HI AGCGCT 1 cut(s) 115
AoxI GGCC 1 cut(s) 228
AspLEI GCGC 1 cut(s) 116
AsuHPI GGTGA 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 247
BccI CCATC 1 cut(s) 76
BciT130I CCWGG 1 cut(s) 212
BclI TGATCA 1 cut(s) 120
BfoI RGCGCY 1 cut(s) 117
BisI GCNGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 229
Bme1390I CCNGG 1 cut(s) 212
BmiI GGNNCC 1 cut(s) 280
BmrFI CCNGG 1 cut(s) 212
BplI GAGNNNNNCTC 2 cut(s) 234, 266
BpmI CTGGAG 1 cut(s) 186
Bsc4I CCNNNNNNNGG 1 cut(s) 237
Bse3DI GCAATG 1 cut(s) 242
BseBI CCWGG 1 cut(s) 212
BseLI CCNNNNNNNGG 1 cut(s) 237
BseMI GCAATG 1 cut(s) 242
BseMII CTCAG 1 cut(s) 86
BshFI GGCC 1 cut(s) 230
BsiHKAI GWGCWC 1 cut(s) 247
BslI CCNNNNNNNGG 1 cut(s) 237
BsnI GGCC 1 cut(s) 230
Bsp1286I GDGCHC 1 cut(s) 247
Bsp143I GATC 2 cut(s) 82, 120
BspACI CCGC 1 cut(s) 227
BspANI GGCC 1 cut(s) 230
BspCNI CTCAG 1 cut(s) 87
BspLI GGNNCC 1 cut(s) 280
BspPI GGATC 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 242
BssMI GATC 2 cut(s) 82, 120
Bst2UI CCWGG 1 cut(s) 212
BstC8I GCNNGC 2 cut(s) 102, 217
BstDEI CTNAG 2 cut(s) 95, 172
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 2 cut(s) 85, 123
BstMBI GATC 2 cut(s) 82, 120
BstNI CCWGG 1 cut(s) 212
BstSCI CCNGG 1 cut(s) 210
BsuRI GGCC 1 cut(s) 230
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 198
Cac8I GCNNGC 2 cut(s) 102, 217
CfoI GCGC 1 cut(s) 116
Csp6I GTAC 2 cut(s) 4, 37
CspCI CAANNNNNGTGG 1 cut(s) 36
CviJI RGCY 4 cut(s) 130, 171, 230, 296
CviKI_1 RGCY 4 cut(s) 130, 171, 230, 296
CviQI GTAC 2 cut(s) 4, 37
DdeI CTNAG 2 cut(s) 95, 172
DpnI GATC 2 cut(s) 84, 122
DpnII GATC 2 cut(s) 82, 120
EaeI YGGCCR 1 cut(s) 228
Eco47III AGCGCT 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 210
FaiI YATR 2 cut(s) 79, 81
FauNDI CATATG 1 cut(s) 79
FbaI TGATCA 1 cut(s) 120
Fnu4HI GCNGC 1 cut(s) 228
Fsp4HI GCNGC 1 cut(s) 228
GlaI GCGC 1 cut(s) 115
GluI GCNGC 1 cut(s) 228
GsuI CTGGAG 1 cut(s) 186
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 1 cut(s) 230
HhaI GCGC 1 cut(s) 116
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HinfI GANTC 2 cut(s) 59, 176
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 2 cut(s) 23, 54
Hpy188I TCNGA 1 cut(s) 96
Hpy188III TCNNGA 1 cut(s) 165
Hpy8I GTNNAC 2 cut(s) 23, 54
HpyAV CCTTC 1 cut(s) 286
HpyF3I CTNAG 2 cut(s) 95, 172
HspAI GCGC 1 cut(s) 114
Ksp22I TGATCA 1 cut(s) 120
Kzo9I GATC 2 cut(s) 82, 120
LmnI GCTCC 1 cut(s) 242
LpnPI CCDG 7 cut(s) 3, 150, 197, 201, 224, 282, 297
MaeIII GTNAC 1 cut(s) 206
MalI GATC 2 cut(s) 84, 122
MboI GATC 2 cut(s) 82, 120
MboII GAAGA 1 cut(s) 149
MhlI GDGCHC 1 cut(s) 247
MlyI GAGTC 1 cut(s) 68
MmeI TCCRAC 1 cut(s) 247
MnlI CCTC 6 cut(s) 24, 90, 96, 161, 202, 268
MspR9I CCNGG 1 cut(s) 212
MvaI CCWGG 1 cut(s) 212
NdeI CATATG 1 cut(s) 79
NdeII GATC 2 cut(s) 82, 120
NlaIV GGNNCC 1 cut(s) 280
NmuCI GTSAC 1 cut(s) 206
PfeI GAWTC 1 cut(s) 176
PflMI CCANNNNNTGG 1 cut(s) 237
PkrI GCNGC 1 cut(s) 229
PleI GAGTC 1 cut(s) 67
PpsI GAGTC 1 cut(s) 67
Psp6I CCWGG 1 cut(s) 210
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 1 cut(s) 280
RsaI GTAC 2 cut(s) 5, 38
RsaNI GTAC 2 cut(s) 4, 37
SatI GCNGC 1 cut(s) 228
Sau3AI GATC 2 cut(s) 82, 120
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 1 cut(s) 212
SduI GDGCHC 1 cut(s) 247
SetI ASST 3 cut(s) 22, 35, 213
SmlI CTYRAG 1 cut(s) 7
SmoI CTYRAG 1 cut(s) 7
SsiI CCGC 1 cut(s) 227
StyD4I CCNGG 1 cut(s) 210
TatI WGTACW 1 cut(s) 3
TauI GCSGC 1 cut(s) 230
TfiI GAWTC 1 cut(s) 176
TscAI CASTG 1 cut(s) 205
TseFI GTSAC 1 cut(s) 206
Tsp45I GTSAC 1 cut(s) 206
TspDTI ATGAA 1 cut(s) 168
TspRI CASTG 1 cut(s) 205
Van91I CCANNNNNTGG 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.