Rh5DG490200

Asparagine synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010661
Physical Location & Seq
Reverse (-)
77023602 .. 77027903
4302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG490200.1

Sequence Viewer

Length: 738 bp
ATGTGTGGAATACTTGCCGTTCTGGGTTGCTCCGATGACTCTCAGGCCAAAAGGGTCAGGGTTCTCGAGCTTTCTCGCAGGTTGAAGCACCGGGGTCCAGATTGGAGTGGTCTGTATCAGCATGGTGATTGTTTCTTGGCCCATCAGAGGCTGGCAATTATTGATCCTGCTTCTGGAGATCAACCTCTGTTTAATGAAGACAAATCAATTGTTGTCACGGTCAATGGAGAGATTTACAACCATGAAGAACTGAGGAAGCGTCTGCCGAATCACAAGTTCCGAACCGGCAGTGATTGTGATGTCATTGCCCATCTGTATGAAGAGTATGGGGAGAACTTTGTGGACATGCTTGATGGCATGTTCTCTTTTGTGCTGCTGGATACCCGGGACAACAGCTTCATTGTTGCTCGTGATGCTATTGGAATCACTTCCCTCTACATGGGCTGGAGTCTTGATGGCTCAATTTGGATTTCATCAGAACTGAAAGGATTGAATGATGATTGTGAACACTTCGAGTCCTTTCCACCTGGTCACCTGTACTCAAGCAAAACAGGTGGACTAAAGAGGTGGTACAACCCCCAATGGTTCACAGAGTCCATTCCATCTACGCCATATGATCCTCTTGTTCTGAGGCGTGCCTTTGAAAGCGTAAATTTCATCACTTGCAACTCATATTTAGAATTTATTTTGATAGGTTGCTGTGTTACTTGTACATGTCAAAGAGAAATTTGTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00250 ko01100 ko01110 map00250 map01100 map01110
Pfam Domains
Protein Families
Asn_synthase GATase_7

Protein Analysis

245

Amino Acids

27.89

Weight (kDa)

5.23

Isoelectric Point (pI)

37.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase_6 PF13522 33 - 160 2.7e-34 Glutamine amidotransferase domain
GATase_7 PF13537 48 - 165 1.3e-40 Glutamine amidotransferase domain
DUF3700 PF12481 112 - 202 1.5e-12 Aluminium induced protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 69
AclWI GGATC 2 cut(s) 158, 611
AcsI RAATTY 3 cut(s) 652, 680, 726
AfaI GTAC 3 cut(s) 539, 572, 712
AfiI CCNNNNNNNGG 3 cut(s) 147, 173, 439
AflIII ACRYGT 1 cut(s) 713
AgsI TTSAA 3 cut(s) 85, 493, 644
AjnI CCWGG 1 cut(s) 526
AluBI AGCT 2 cut(s) 70, 396
AluI AGCT 2 cut(s) 70, 396
AlwI GGATC 2 cut(s) 158, 611
AlwNI CAGNNNCTG 1 cut(s) 151
Ama87I CYCGRG 2 cut(s) 65, 384
AoxI GGCC 2 cut(s) 45, 138
ApeKI GCWGC 1 cut(s) 373
ApoI RAATTY 3 cut(s) 652, 680, 726
Asp700I GAANNNNTTC 1 cut(s) 427
AspS9I GGNCC 2 cut(s) 95, 139
AsuC2I CCSGG 3 cut(s) 92, 385, 386
AsuHPI GGTGA 2 cut(s) 137, 524
AvaI CYCGRG 2 cut(s) 65, 384
AvaII GGWCC 1 cut(s) 95
BauI CACGAG 1 cut(s) 408
BbsI GAAGAC 1 cut(s) 204
BbvI GCAGC 1 cut(s) 360
BccI CCATC 5 cut(s) 150, 318, 347, 449, 610
BceAI ACGGC 1 cut(s) 2
BciT130I CCWGG 1 cut(s) 528
BciVI GTATCC 1 cut(s) 373
BcnI CCSGG 3 cut(s) 92, 385, 386
BfuAI ACCTGC 1 cut(s) 69
BfuI GTATCC 1 cut(s) 373
BisI GCNGC 1 cut(s) 374
BlsI GCNGC 1 cut(s) 375
Bme1390I CCNGG 4 cut(s) 92, 385, 386, 528
Bme18I GGWCC 1 cut(s) 95
BmeT110I CYCGRG 2 cut(s) 65, 384
BmgT120I GGNCC 2 cut(s) 95, 139
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 4 cut(s) 92, 385, 386, 528
BmsI GCATC 1 cut(s) 403
BpiI GAAGAC 1 cut(s) 204
BpmI CTGGAG 2 cut(s) 195, 466
BpuEI CTTGAG 1 cut(s) 526
BpuMI CCSGG 3 cut(s) 92, 385, 386
BsaJI CCNNGG 2 cut(s) 91, 384
Bsc4I CCNNNNNNNGG 3 cut(s) 147, 173, 439
Bse118I RCCGGY 1 cut(s) 284
Bse3DI GCAATG 1 cut(s) 303
BseBI CCWGG 1 cut(s) 528
BseDI CCNNGG 2 cut(s) 91, 384
BseLI CCNNNNNNNGG 3 cut(s) 147, 173, 439
BseMI GCAATG 1 cut(s) 303
BseMII CTCAG 3 cut(s) 56, 242, 620
BseXI GCAGC 1 cut(s) 360
BshFI GGCC 2 cut(s) 47, 140
BsiHKCI CYCGRG 2 cut(s) 65, 384
BsiSI CCGG 3 cut(s) 91, 285, 385
BslFI GGGAC 1 cut(s) 401
BslI CCNNNNNNNGG 3 cut(s) 147, 173, 439
BsmFI GGGAC 1 cut(s) 401
BsnI GGCC 2 cut(s) 47, 140
BsoBI CYCGRG 2 cut(s) 65, 384
Bsp1407I TGTACA 1 cut(s) 710
Bsp143I GATC 3 cut(s) 163, 178, 616
BspANI GGCC 2 cut(s) 47, 140
BspCNI CTCAG 3 cut(s) 55, 243, 621
BspLI GGNNCC 1 cut(s) 96
BspMI ACCTGC 1 cut(s) 69
BspPI GGATC 2 cut(s) 158, 611
BsrDI GCAATG 1 cut(s) 303
BsrFI RCCGGY 1 cut(s) 284
BsrGI TGTACA 1 cut(s) 710
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 2 cut(s) 91, 384
BssMI GATC 3 cut(s) 163, 178, 616
BssSI CACGAG 1 cut(s) 408
Bst2BI CACGAG 1 cut(s) 408
Bst2UI CCWGG 1 cut(s) 528
Bst4CI ACNGT 1 cut(s) 220
Bst6I CTCTTC 1 cut(s) 315
BstAUI TGTACA 1 cut(s) 710
BstC8I GCNNGC 2 cut(s) 153, 636
BstDEI CTNAG 3 cut(s) 42, 251, 629
BstEII GGTNACC 1 cut(s) 530
BstKTI GATC 3 cut(s) 166, 181, 619
BstMBI GATC 3 cut(s) 163, 178, 616
BstMWI GCNNNNNNNGC 1 cut(s) 413
BstNI CCWGG 1 cut(s) 528
BstNSI RCATGY 3 cut(s) 349, 361, 717
BstPI GGTNACC 1 cut(s) 530
BstSCI CCNGG 4 cut(s) 90, 383, 384, 526
BstV1I GCAGC 1 cut(s) 360
BstV2I GAAGAC 1 cut(s) 204
BsuI GTATCC 1 cut(s) 373
BsuRI GGCC 2 cut(s) 47, 140
BtsI GCAGTG 1 cut(s) 295
BtsIMutI CAGTG 1 cut(s) 295
BveI ACCTGC 1 cut(s) 69
Cac8I GCNNGC 2 cut(s) 153, 636
CaiI CAGNNNCTG 1 cut(s) 151
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 2 cut(s) 95, 139
Cfr9I CCCGGG 1 cut(s) 384
CseI GACGC 1 cut(s) 248
CsiI ACCWGGT 1 cut(s) 526
Csp6I GTAC 3 cut(s) 538, 571, 711
CspCI CAANNNNNGTGG 2 cut(s) 535, 570
CviAII CATG 6 cut(s) 122, 242, 346, 358, 439, 714
CviJI RGCY 7 cut(s) 47, 70, 140, 151, 396, 444, 459
CviKI_1 RGCY 7 cut(s) 47, 70, 140, 151, 396, 444, 459
CviQI GTAC 3 cut(s) 538, 571, 711
DdeI CTNAG 3 cut(s) 42, 251, 629
DpnI GATC 3 cut(s) 165, 180, 618
DpnII GATC 3 cut(s) 163, 178, 616
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
Eco47I GGWCC 1 cut(s) 95
Eco88I CYCGRG 2 cut(s) 65, 384
Eco91I GGTNACC 1 cut(s) 530
EcoO65I GGTNACC 1 cut(s) 530
EcoRII CCWGG 1 cut(s) 526
FaeI CATG 6 cut(s) 125, 245, 349, 361, 442, 717
FaqI GGGAC 1 cut(s) 401
FatI CATG 6 cut(s) 121, 241, 345, 357, 438, 713
FauNDI CATATG 1 cut(s) 613
Fnu4HI GCNGC 1 cut(s) 374
Fsp4HI GCNGC 1 cut(s) 374
GluI GCNGC 1 cut(s) 374
GsuI CTGGAG 2 cut(s) 195, 466
HaeIII GGCC 2 cut(s) 47, 140
HapII CCGG 3 cut(s) 91, 285, 385
HgaI GACGC 1 cut(s) 248
Hin1II CATG 6 cut(s) 125, 245, 349, 361, 442, 717
HinfI GANTC 6 cut(s) 38, 268, 423, 448, 515, 593
HpaII CCGG 3 cut(s) 91, 285, 385
HphI GGTGA 2 cut(s) 137, 524
Hpy166II GTNNAC 4 cut(s) 343, 506, 557, 588
Hpy188I TCNGA 5 cut(s) 34, 147, 281, 478, 630
Hpy188III TCNNGA 5 cut(s) 65, 98, 174, 410, 452
Hpy8I GTNNAC 4 cut(s) 343, 506, 557, 588
HpyCH4III ACNGT 1 cut(s) 220
HpyCH4V TGCA 1 cut(s) 666
HpyF10VI GCNNNNNNNGC 1 cut(s) 413
HpyF3I CTNAG 3 cut(s) 42, 251, 629
Hsp92II CATG 6 cut(s) 125, 245, 349, 361, 442, 717
Kzo9I GATC 3 cut(s) 163, 178, 616
LmnI GCTCC 1 cut(s) 35
Lsp1109I GCAGC 1 cut(s) 360
LweI GCATC 1 cut(s) 403
MabI ACCWGGT 1 cut(s) 526
MaeIII GTNAC 3 cut(s) 214, 530, 703
MalI GATC 3 cut(s) 165, 180, 618
MboI GATC 3 cut(s) 163, 178, 616
MboII GAAGA 3 cut(s) 209, 257, 332
MfeI CAATTG 1 cut(s) 207
MluCI AATT 7 cut(s) 156, 207, 462, 652, 680, 726, 733
MlyI GAGTC 4 cut(s) 32, 457, 524, 602
MnlI CCTC 7 cut(s) 141, 195, 246, 443, 558, 624, 630
MroXI GAANNNNTTC 1 cut(s) 427
MseI TTAA 2 cut(s) 192, 736
MslI CAYNNNNRTG 1 cut(s) 315
MspI CCGG 3 cut(s) 91, 285, 385
MspR9I CCNGG 4 cut(s) 92, 385, 386, 528
MunI CAATTG 1 cut(s) 207
MvaI CCWGG 1 cut(s) 528
MwoI GCNNNNNNNGC 1 cut(s) 413
NciI CCSGG 3 cut(s) 92, 385, 386
NdeI CATATG 1 cut(s) 613
NdeII GATC 3 cut(s) 163, 178, 616
NlaIII CATG 6 cut(s) 125, 245, 349, 361, 442, 717
NlaIV GGNNCC 1 cut(s) 96
NmuCI GTSAC 2 cut(s) 214, 530
NspI RCATGY 3 cut(s) 349, 361, 717
PaeR7I CTCGAG 1 cut(s) 65
PciI ACATGT 1 cut(s) 713
PdmI GAANNNNTTC 1 cut(s) 427
PfeI GAWTC 2 cut(s) 268, 423
PkrI GCNGC 1 cut(s) 375
PleI GAGTC 4 cut(s) 32, 456, 523, 601
PpsI GAGTC 4 cut(s) 32, 456, 523, 601
PscI ACATGT 1 cut(s) 713
Psp6I CCWGG 1 cut(s) 526
PspEI GGTNACC 1 cut(s) 530
PspGI CCWGG 1 cut(s) 526
PspN4I GGNNCC 1 cut(s) 96
PspPI GGNCC 2 cut(s) 95, 139
PstNI CAGNNNCTG 1 cut(s) 151
RsaI GTAC 3 cut(s) 539, 572, 712
RsaNI GTAC 3 cut(s) 538, 571, 711
RseI CAYNNNNRTG 1 cut(s) 315
SaqAI TTAA 2 cut(s) 192, 736
SatI GCNGC 1 cut(s) 374
Sau3AI GATC 3 cut(s) 163, 178, 616
Sau96I GGNCC 2 cut(s) 95, 139
SchI GAGTC 4 cut(s) 32, 457, 524, 602
ScrFI CCNGG 4 cut(s) 92, 385, 386, 528
SetI ASST 9 cut(s) 72, 83, 187, 398, 529, 537, 556, 569, 697
SexAI ACCWGGT 1 cut(s) 526
SfaNI GCATC 1 cut(s) 403
Sfr274I CTCGAG 1 cut(s) 65
SinI GGWCC 1 cut(s) 95
SlaI CTCGAG 1 cut(s) 65
SmaI CCCGGG 1 cut(s) 386
SmiMI CAYNNNNRTG 1 cut(s) 315
SmlI CTYRAG 2 cut(s) 65, 541
SmoI CTYRAG 2 cut(s) 65, 541
Sse9I AATT 7 cut(s) 156, 207, 462, 652, 680, 726, 733
StyD4I CCNGG 4 cut(s) 90, 383, 384, 526
TaaI ACNGT 1 cut(s) 220
TaqI TCGA 2 cut(s) 66, 513
TasI AATT 7 cut(s) 156, 207, 462, 652, 680, 726, 733
TatI WGTACW 2 cut(s) 537, 710
TfiI GAWTC 2 cut(s) 268, 423
Tru1I TTAA 2 cut(s) 192, 736
Tru9I TTAA 2 cut(s) 192, 736
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 2 cut(s) 214, 530
TseI GCWGC 1 cut(s) 373
Tsp45I GTSAC 2 cut(s) 214, 530
TspDTI ATGAA 6 cut(s) 210, 258, 333, 388, 462, 646
TspMI CCCGGG 1 cut(s) 384
TspRI CASTG 1 cut(s) 295
VpaK11BI GGWCC 1 cut(s) 95
XapI RAATTY 3 cut(s) 652, 680, 726
XceI RCATGY 3 cut(s) 349, 361, 717
XhoI CTCGAG 1 cut(s) 65
XmaI CCCGGG 1 cut(s) 384
XmnI GAANNNNTTC 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.